AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0775700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0775700 MtrunA17_Chr5g0430231 90.312 320 22 5 25 338 27 343 0.0 512
Msa0775700 MtrunA17_Chr3g0105161 35.294 391 163 12 26 335 27 408 2.30e-47 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATGAGTGTGTAGATATT+TGG 0.183054 5_2:-52825267 Msa0775700:CDS
TGGTGTAGTTGCAAGGTTAA+TGG 0.255423 5_2:-52826261 Msa0775700:CDS
AAGAGTTTGTTTAAGGAAGT+TGG 0.287665 5_2:-52825984 Msa0775700:CDS
GGTGTAGTTGCAAGGTTAAT+GGG 0.347018 5_2:-52826260 Msa0775700:CDS
TGAAGAAGAGAGAAAAGTTA+AGG 0.378603 5_2:-52825338 Msa0775700:CDS
TCAAAATCAAAGACAGAAAC+AGG 0.397845 5_2:+52825870 None:intergenic
GAGCATGATGTTTCTGGAAC+AGG 0.399353 5_2:-52825849 Msa0775700:intron
AGTTGCAAGGTTAATGGGAT+TGG 0.401363 5_2:-52826255 Msa0775700:CDS
CAAGCTAGTTGATGATGAAT+TGG 0.402114 5_2:-52825233 Msa0775700:CDS
CAAAGGAAGAAGAAAAGAAA+AGG 0.402477 5_2:-52826075 Msa0775700:CDS
GAAATTATAGTTCTAGTAGT+AGG 0.402687 5_2:+52826296 None:intergenic
GTTAATGGGATTGGATTCAA+TGG 0.432909 5_2:-52826246 Msa0775700:CDS
ATTGAGAACAATTTGAATGA+AGG 0.441642 5_2:-52825313 Msa0775700:CDS
CTTCTTCTGGTGTAGTTGCA+AGG 0.448678 5_2:-52826268 Msa0775700:CDS
TTGTTTAAGGAAGTTGGAAA+TGG 0.464241 5_2:-52825978 Msa0775700:CDS
ATTATAGTTCTAGTAGTAGG+AGG 0.474477 5_2:+52826299 None:intergenic
TTTGTGATGTTGATATGAGT+TGG 0.497412 5_2:-52825432 Msa0775700:CDS
GTGAATTGCATGAAGTAAAG+AGG 0.512937 5_2:-52825201 Msa0775700:CDS
TTGCAATGTGTAAGTGAACT+TGG 0.522297 5_2:+52826197 None:intergenic
GGATTGGATTCAATGGAAGA+AGG 0.529487 5_2:-52826239 Msa0775700:CDS
CTAGTTGATGATGAATTGGT+TGG 0.537429 5_2:-52825229 Msa0775700:CDS
CAGAATGTTGAATAAAGAGC+TGG 0.546875 5_2:+52825377 None:intergenic
AGTAGAAAATTCAAGTCCAA+AGG 0.548034 5_2:-52826092 Msa0775700:CDS
AAAGGAAGAAGAAAAGAAAA+GGG 0.548330 5_2:-52826074 Msa0775700:CDS
GATTGGATTCAATGGAAGAA+GGG 0.549935 5_2:-52826238 Msa0775700:CDS
TTGGTGTTTAGCTTTGAAAG+TGG 0.552499 5_2:-52826122 Msa0775700:CDS
TTATAGTTCTAGTAGTAGGA+GGG 0.559285 5_2:+52826300 None:intergenic
TCAAGCAAATGAAAAGCTTG+TGG 0.565993 5_2:+52826158 None:intergenic
AACATGTTGTGTTGAAGAGA+AGG 0.571785 5_2:-52825925 Msa0775700:CDS
CTAAAGTTTGATGAAGATGA+TGG 0.588538 5_2:+52826449 None:intergenic
AGAGAAAAGGTGAATGATGA+TGG 0.597704 5_2:-52826023 Msa0775700:CDS
GTGTTTAGCTTTGAAAGTGG+AGG 0.617135 5_2:-52826119 Msa0775700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTATTTCCAAAAAATAAAA+AGG + chr5_2:52825958-52825977 None:intergenic 15.0%
!! ACTATTTCCAAAAAATAAAA+AGG + chr5_2:52825958-52825977 None:intergenic 15.0%
!!! ATTATTTCAACAAGCTTTTA+AGG - chr5_2:52825878-52825897 Msa0775700:CDS 20.0%
!!! GATCTTACCTTTTTATTTTT+TGG - chr5_2:52825948-52825967 Msa0775700:CDS 20.0%
!! CAAATTTAATACATGGAAAA+CGG + chr5_2:52826092-52826111 None:intergenic 20.0%
!! TAGTTAGCAAATTTAATACA+TGG + chr5_2:52826099-52826118 None:intergenic 20.0%
!!! ATTATTTCAACAAGCTTTTA+AGG - chr5_2:52825878-52825897 Msa0775700:CDS 20.0%
!!! GATCTTACCTTTTTATTTTT+TGG - chr5_2:52825948-52825967 Msa0775700:CDS 20.0%
!! CAAATTTAATACATGGAAAA+CGG + chr5_2:52826092-52826111 None:intergenic 20.0%
!! TAGTTAGCAAATTTAATACA+TGG + chr5_2:52826099-52826118 None:intergenic 20.0%
! TTCTCTAATCCTATGAAAAA+TGG + chr5_2:52825214-52825233 None:intergenic 25.0%
!!! GAAATTATAGTTCTAGTAGT+AGG + chr5_2:52825291-52825310 None:intergenic 25.0%
! ATAATTTCTTCTTCTTCTTC+TGG - chr5_2:52825303-52825322 Msa0775700:CDS 25.0%
! AAAGGAAGAAGAAAAGAAAA+GGG - chr5_2:52825510-52825529 Msa0775700:intron 25.0%
!!! TTTTGAGAAGAGTTTGTTTA+AGG - chr5_2:52825593-52825612 Msa0775700:intron 25.0%
! AATTTAATGCATGTTTGGAT+TGG - chr5_2:52825806-52825825 Msa0775700:intron 25.0%
! ATAAACAATTAGGTCATGTT+TGG + chr5_2:52826019-52826038 None:intergenic 25.0%
! TTGTGTTTGCATAAACAATT+AGG + chr5_2:52826029-52826048 None:intergenic 25.0%
! CACTATGTATCTAAATTCTA+AGG + chr5_2:52826056-52826075 None:intergenic 25.0%
! ATTGAGAACAATTTGAATGA+AGG - chr5_2:52826271-52826290 Msa0775700:CDS 25.0%
! TTCTCTAATCCTATGAAAAA+TGG + chr5_2:52825214-52825233 None:intergenic 25.0%
!!! GAAATTATAGTTCTAGTAGT+AGG + chr5_2:52825291-52825310 None:intergenic 25.0%
! ATAATTTCTTCTTCTTCTTC+TGG - chr5_2:52825303-52825322 Msa0775700:CDS 25.0%
! AAAGGAAGAAGAAAAGAAAA+GGG - chr5_2:52825510-52825529 Msa0775700:intron 25.0%
!!! TTTTGAGAAGAGTTTGTTTA+AGG - chr5_2:52825593-52825612 Msa0775700:intron 25.0%
! AATTTAATGCATGTTTGGAT+TGG - chr5_2:52825806-52825825 Msa0775700:intron 25.0%
! ATAAACAATTAGGTCATGTT+TGG + chr5_2:52826019-52826038 None:intergenic 25.0%
! TTGTGTTTGCATAAACAATT+AGG + chr5_2:52826029-52826048 None:intergenic 25.0%
! CACTATGTATCTAAATTCTA+AGG + chr5_2:52826056-52826075 None:intergenic 25.0%
! ATTGAGAACAATTTGAATGA+AGG - chr5_2:52826271-52826290 Msa0775700:CDS 25.0%
CTAAAGTTTGATGAAGATGA+TGG + chr5_2:52825138-52825157 None:intergenic 30.0%
!! GTTTCACAACCATTTTTCAT+AGG - chr5_2:52825202-52825221 Msa0775700:CDS 30.0%
!! TTATAGTTCTAGTAGTAGGA+GGG + chr5_2:52825287-52825306 None:intergenic 30.0%
!! ATTATAGTTCTAGTAGTAGG+AGG + chr5_2:52825288-52825307 None:intergenic 30.0%
! GCTTGAAAATGAGAACTTTT+TGG - chr5_2:52825443-52825462 Msa0775700:CDS 30.0%
AGTAGAAAATTCAAGTCCAA+AGG - chr5_2:52825492-52825511 Msa0775700:intron 30.0%
! CTTTTCTTTTCTTCTTCCTT+TGG + chr5_2:52825511-52825530 None:intergenic 30.0%
CAAAGGAAGAAGAAAAGAAA+AGG - chr5_2:52825509-52825528 Msa0775700:intron 30.0%
GAAGAAAAACAAGAGAGAAA+AGG - chr5_2:52825548-52825567 Msa0775700:intron 30.0%
! AAGAGTTTGTTTAAGGAAGT+TGG - chr5_2:52825600-52825619 Msa0775700:intron 30.0%
TTGTTTAAGGAAGTTGGAAA+TGG - chr5_2:52825606-52825625 Msa0775700:intron 30.0%
TCAAAATCAAAGACAGAAAC+AGG + chr5_2:52825717-52825736 None:intergenic 30.0%
CCAAACATGCATTAAATTTG+AGG + chr5_2:52825804-52825823 None:intergenic 30.0%
CCTCAAATTTAATGCATGTT+TGG - chr5_2:52825801-52825820 Msa0775700:intron 30.0%
CTATTGAAATGCTCATAAGT+TGG - chr5_2:52825849-52825868 Msa0775700:intron 30.0%
TTAAGGATAGCTTACGAAAA+CGG - chr5_2:52825895-52825914 Msa0775700:CDS 30.0%
CATATATAAACATGTTGCAG+AGG - chr5_2:52826123-52826142 Msa0775700:CDS 30.0%
TTTGTGATGTTGATATGAGT+TGG - chr5_2:52826152-52826171 Msa0775700:CDS 30.0%
TGAAGAAGAGAGAAAAGTTA+AGG - chr5_2:52826246-52826265 Msa0775700:CDS 30.0%
AAGGATCAAAGAAAAAAGAG+AGG - chr5_2:52826290-52826309 Msa0775700:CDS 30.0%
ACAATGAGTGTGTAGATATT+TGG - chr5_2:52826317-52826336 Msa0775700:CDS 30.0%
CTAAAGTTTGATGAAGATGA+TGG + chr5_2:52825138-52825157 None:intergenic 30.0%
!! GTTTCACAACCATTTTTCAT+AGG - chr5_2:52825202-52825221 Msa0775700:CDS 30.0%
!! TTATAGTTCTAGTAGTAGGA+GGG + chr5_2:52825287-52825306 None:intergenic 30.0%
!! ATTATAGTTCTAGTAGTAGG+AGG + chr5_2:52825288-52825307 None:intergenic 30.0%
! GCTTGAAAATGAGAACTTTT+TGG - chr5_2:52825443-52825462 Msa0775700:CDS 30.0%
AGTAGAAAATTCAAGTCCAA+AGG - chr5_2:52825492-52825511 Msa0775700:intron 30.0%
! CTTTTCTTTTCTTCTTCCTT+TGG + chr5_2:52825511-52825530 None:intergenic 30.0%
CAAAGGAAGAAGAAAAGAAA+AGG - chr5_2:52825509-52825528 Msa0775700:intron 30.0%
GAAGAAAAACAAGAGAGAAA+AGG - chr5_2:52825548-52825567 Msa0775700:intron 30.0%
! AAGAGTTTGTTTAAGGAAGT+TGG - chr5_2:52825600-52825619 Msa0775700:intron 30.0%
TTGTTTAAGGAAGTTGGAAA+TGG - chr5_2:52825606-52825625 Msa0775700:intron 30.0%
TCAAAATCAAAGACAGAAAC+AGG + chr5_2:52825717-52825736 None:intergenic 30.0%
CCAAACATGCATTAAATTTG+AGG + chr5_2:52825804-52825823 None:intergenic 30.0%
CCTCAAATTTAATGCATGTT+TGG - chr5_2:52825801-52825820 Msa0775700:intron 30.0%
CTATTGAAATGCTCATAAGT+TGG - chr5_2:52825849-52825868 Msa0775700:intron 30.0%
TTAAGGATAGCTTACGAAAA+CGG - chr5_2:52825895-52825914 Msa0775700:CDS 30.0%
CATATATAAACATGTTGCAG+AGG - chr5_2:52826123-52826142 Msa0775700:CDS 30.0%
TTTGTGATGTTGATATGAGT+TGG - chr5_2:52826152-52826171 Msa0775700:CDS 30.0%
TGAAGAAGAGAGAAAAGTTA+AGG - chr5_2:52826246-52826265 Msa0775700:CDS 30.0%
AAGGATCAAAGAAAAAAGAG+AGG - chr5_2:52826290-52826309 Msa0775700:CDS 30.0%
ACAATGAGTGTGTAGATATT+TGG - chr5_2:52826317-52826336 Msa0775700:CDS 30.0%
GTTAATGGGATTGGATTCAA+TGG - chr5_2:52825338-52825357 Msa0775700:CDS 35.0%
GATTGGATTCAATGGAAGAA+GGG - chr5_2:52825346-52825365 Msa0775700:CDS 35.0%
TTGCAATGTGTAAGTGAACT+TGG + chr5_2:52825390-52825409 None:intergenic 35.0%
TCAAGCAAATGAAAAGCTTG+TGG + chr5_2:52825429-52825448 None:intergenic 35.0%
!! TTGGTGTTTAGCTTTGAAAG+TGG - chr5_2:52825462-52825481 Msa0775700:intron 35.0%
AGAGAAAAGGTGAATGATGA+TGG - chr5_2:52825561-52825580 Msa0775700:intron 35.0%
AACATGTTGTGTTGAAGAGA+AGG - chr5_2:52825659-52825678 Msa0775700:intron 35.0%
! GATTTTGAGCATGATGTTTC+TGG - chr5_2:52825729-52825748 Msa0775700:intron 35.0%
CAGAATGTTGAATAAAGAGC+TGG + chr5_2:52826210-52826229 None:intergenic 35.0%
!! CAAGCTAGTTGATGATGAAT+TGG - chr5_2:52826351-52826370 Msa0775700:CDS 35.0%
!! CTAGTTGATGATGAATTGGT+TGG - chr5_2:52826355-52826374 Msa0775700:CDS 35.0%
GTGAATTGCATGAAGTAAAG+AGG - chr5_2:52826383-52826402 Msa0775700:CDS 35.0%
GTTAATGGGATTGGATTCAA+TGG - chr5_2:52825338-52825357 Msa0775700:CDS 35.0%
GATTGGATTCAATGGAAGAA+GGG - chr5_2:52825346-52825365 Msa0775700:CDS 35.0%
TTGCAATGTGTAAGTGAACT+TGG + chr5_2:52825390-52825409 None:intergenic 35.0%
TCAAGCAAATGAAAAGCTTG+TGG + chr5_2:52825429-52825448 None:intergenic 35.0%
!! TTGGTGTTTAGCTTTGAAAG+TGG - chr5_2:52825462-52825481 Msa0775700:intron 35.0%
AGAGAAAAGGTGAATGATGA+TGG - chr5_2:52825561-52825580 Msa0775700:intron 35.0%
AACATGTTGTGTTGAAGAGA+AGG - chr5_2:52825659-52825678 Msa0775700:intron 35.0%
! GATTTTGAGCATGATGTTTC+TGG - chr5_2:52825729-52825748 Msa0775700:intron 35.0%
CAGAATGTTGAATAAAGAGC+TGG + chr5_2:52826210-52826229 None:intergenic 35.0%
!! CAAGCTAGTTGATGATGAAT+TGG - chr5_2:52826351-52826370 Msa0775700:CDS 35.0%
!! CTAGTTGATGATGAATTGGT+TGG - chr5_2:52826355-52826374 Msa0775700:CDS 35.0%
GTGAATTGCATGAAGTAAAG+AGG - chr5_2:52826383-52826402 Msa0775700:CDS 35.0%
TGGTGTAGTTGCAAGGTTAA+TGG - chr5_2:52825323-52825342 Msa0775700:CDS 40.0%
GGTGTAGTTGCAAGGTTAAT+GGG - chr5_2:52825324-52825343 Msa0775700:CDS 40.0%
AGTTGCAAGGTTAATGGGAT+TGG - chr5_2:52825329-52825348 Msa0775700:CDS 40.0%
GGATTGGATTCAATGGAAGA+AGG - chr5_2:52825345-52825364 Msa0775700:CDS 40.0%
!! GTGTTTAGCTTTGAAAGTGG+AGG - chr5_2:52825465-52825484 Msa0775700:intron 40.0%
TGGTGTAGTTGCAAGGTTAA+TGG - chr5_2:52825323-52825342 Msa0775700:CDS 40.0%
GGTGTAGTTGCAAGGTTAAT+GGG - chr5_2:52825324-52825343 Msa0775700:CDS 40.0%
AGTTGCAAGGTTAATGGGAT+TGG - chr5_2:52825329-52825348 Msa0775700:CDS 40.0%
GGATTGGATTCAATGGAAGA+AGG - chr5_2:52825345-52825364 Msa0775700:CDS 40.0%
!! GTGTTTAGCTTTGAAAGTGG+AGG - chr5_2:52825465-52825484 Msa0775700:intron 40.0%
AGCTCAAAAACTGTTGCTGC+AGG - chr5_2:52825177-52825196 Msa0775700:CDS 45.0%
GCTCAAAAACTGTTGCTGCA+GGG - chr5_2:52825178-52825197 Msa0775700:CDS 45.0%
CTTCTTCTGGTGTAGTTGCA+AGG - chr5_2:52825316-52825335 Msa0775700:CDS 45.0%
!! GAGCATGATGTTTCTGGAAC+AGG - chr5_2:52825735-52825754 Msa0775700:intron 45.0%
!! AGCTGGTCATTTTCAAGCAC+TGG + chr5_2:52826193-52826212 None:intergenic 45.0%
AGCTCAAAAACTGTTGCTGC+AGG - chr5_2:52825177-52825196 Msa0775700:CDS 45.0%
GCTCAAAAACTGTTGCTGCA+GGG - chr5_2:52825178-52825197 Msa0775700:CDS 45.0%
CTTCTTCTGGTGTAGTTGCA+AGG - chr5_2:52825316-52825335 Msa0775700:CDS 45.0%
!! GAGCATGATGTTTCTGGAAC+AGG - chr5_2:52825735-52825754 Msa0775700:intron 45.0%
!! AGCTGGTCATTTTCAAGCAC+TGG + chr5_2:52826193-52826212 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 52825102 52826504 52825102 ID=Msa0775700;Name=Msa0775700
chr5_2 mRNA 52825102 52826504 52825102 ID=Msa0775700-mRNA-1;Parent=Msa0775700;Name=Msa0775700-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|1|0|1|1|1|2|0|338
chr5_2 exon 52825850 52826504 52825850 ID=Msa0775700-mRNA-1:exon:15796;Parent=Msa0775700-mRNA-1
chr5_2 exon 52825102 52825463 52825102 ID=Msa0775700-mRNA-1:exon:15795;Parent=Msa0775700-mRNA-1
chr5_2 CDS 52825850 52826504 52825850 ID=Msa0775700-mRNA-1:cds;Parent=Msa0775700-mRNA-1
chr5_2 CDS 52825102 52825463 52825102 ID=Msa0775700-mRNA-1:cds;Parent=Msa0775700-mRNA-1
Gene Sequence

>Msa0775700

ATGAAGCTCTTGATATCATCTTATTCATCATCACCATCATCTTCATCAAACTTTAGCTTTGACTCAACATTTTGCAGCTCAAAAACTGTTGCTGCAGGGTGTTTCACAACCATTTTTCATAGGATTAGAGAATTTCATTCAAAGTTCATTGTAAGTGAAAAAGTTCAAAAATTGAAGACTAACCCTCCTACTACTAGAACTATAATTTCTTCTTCTTCTTCTGGTGTAGTTGCAAGGTTAATGGGATTGGATTCAATGGAAGAAGGGATAGTTTCTGAATCAAAACCAAGTTCACTTACACATTGCAAGTCATTGAACTATGTGCCACAAGCTTTTCATTTGCTTGAAAATGAGAACTTTTTGGTGTTTAGCTTTGAAAGTGGAGGTGAGAGTAGAAAATTCAAGTCCAAAGGAAGAAGAAAAGAAAAGGGTTGTGTTGAATTGAAGAAGAAAAACAAGAGAGAAAAGGTGAATGATGATGGTGAATTTCATTTTGAGAAGAGTTTGTTTAAGGAAGTTGGAAATGGTGAGAAAGTGAAGAAGAGAAAGAAAGAAAAAACATGTTGTGTTGAAGAGAAGGTTGAAAGTGAATGTAGTTCAGAAGATTATAGTCCTGTTTCTGTCTTTGATTTTGAGCATGATGTTTCTGGAACAGAGGAAGATCTTTGTGATGTTGATATGAGTTGGAGAAGAAAATTGTCACCAGTGCTTGAAAATGACCAGCTCTTTATTCAACATTCTGATAGTAACTTAATAAATGAAGAAGAGAGAAAAGTTAAGGAAATTGAGAACAATTTGAATGAAGGATCAAAGAAAAAAGAGAGGCATAACAATGAGTGTGTAGATATTTGGATTAAAATTTGCAAGCTAGTTGATGATGAATTGGTTGGATCAAGTGAATTGCATGAAGTAAAGAGGAAACAAAATGATTTAGAAAATATAAGTGCTGATTTTGAGTTAGAGATTTTAGATGAATTGTTAGATGAAGTTATAGATCAATTTGTTAGTTGTTTGTAA

Protein sequence

>Msa0775700

MKLLISSYSSSPSSSSNFSFDSTFCSSKTVAAGCFTTIFHRIREFHSKFIVSEKVQKLKTNPPTTRTIISSSSSGVVARLMGLDSMEEGIVSESKPSSLTHCKSLNYVPQAFHLLENENFLVFSFESGGESRKFKSKGRRKEKGCVELKKKNKREKVNDDGEFHFEKSLFKEVGNGEKVKKRKKEKTCCVEEKVESECSSEDYSPVSVFDFEHDVSGTEEDLCDVDMSWRRKLSPVLENDQLFIQHSDSNLINEEERKVKEIENNLNEGSKKKERHNNECVDIWIKICKLVDDELVGSSELHEVKRKQNDLENISADFELEILDELLDEVIDQFVSCL*