AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0826130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826130 MtrunA17_Chr5g0448181 89.333 75 7 1 1 74 1 75 7.06e-32 105
Msa0826130 MtrunA17_Chr3g0078121 88.000 75 8 1 1 74 1 75 4.87e-31 103
Msa0826130 MtrunA17_Chr3g0078141 74.545 55 13 1 4 57 1 55 1.21e-23 84.3
Msa0826130 MtrunA17_Chr5g0448161 74.545 55 13 1 4 57 1 55 1.21e-23 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826130 AT1G31335.1 80.488 41 8 0 1 41 1 41 2.31e-20 76.6

Find 25 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACACAAACCCTCAAGTTTA+TGG 0.161657 5_3:+66276199 Msa0826130:CDS
ATCTTCTTCTGCTTAATTCT+TGG 0.163666 5_3:+66276130 Msa0826130:CDS
TTCTGCTTAATTCTTGGTTT+TGG 0.239621 5_3:+66276136 Msa0826130:CDS
ATTAAGCAGAAGAAGATTAA+AGG 0.258762 5_3:-66276124 None:intergenic
GGAGCACCTGGTGGTGGAAT+TGG 0.383062 5_3:-66276226 None:intergenic
GTTGGAAGCAAGGTTTGGTT+TGG 0.389775 5_3:-66276293 None:intergenic
CAAACGTTGGAAGCAAGGTT+TGG 0.414160 5_3:-66276298 None:intergenic
GAAGGGAAGGTAGTATTTGC+AGG 0.442054 5_3:-66276247 None:intergenic
AGGTTTGGTTTGGAAAATTG+TGG 0.482075 5_3:-66276283 None:intergenic
GGTTTGGAAAATTGTGGAGG+AGG 0.501357 5_3:-66276277 None:intergenic
TTGGCATACCATAAACTTGA+GGG 0.504350 5_3:-66276207 None:intergenic
TGTGGAGGAGGAGATGATGA+AGG 0.512084 5_3:-66276265 None:intergenic
AGGAGGAGATGATGAAGGGA+AGG 0.519302 5_3:-66276260 None:intergenic
GAACTCAAACGTTGGAAGCA+AGG 0.528191 5_3:-66276303 None:intergenic
AATTAAGATGGGAATGGTAG+TGG 0.531670 5_3:+66276090 None:intergenic
GTAGTATTTGCAGGAGCACC+TGG 0.535931 5_3:-66276238 None:intergenic
TTTGCAGGAGCACCTGGTGG+TGG 0.537498 5_3:-66276232 None:intergenic
GTGGAGGAGGAGATGATGAA+GGG 0.553528 5_3:-66276264 None:intergenic
TACTTCTTTGGAAGAGCTAG+AGG 0.553790 5_3:+66276163 Msa0826130:CDS
TATGAGTTGAACTCAAACGT+TGG 0.555554 5_3:-66276311 None:intergenic
AGAGAAAATTAAGATGGGAA+TGG 0.566014 5_3:+66276084 None:intergenic
TTTGGTTTGGAAAATTGTGG+AGG 0.619984 5_3:-66276280 None:intergenic
GGTATGCCAATTCCACCACC+AGG 0.644332 5_3:+66276220 Msa0826130:CDS
ATTGGCATACCATAAACTTG+AGG 0.668164 5_3:-66276208 None:intergenic
GTATTTGCAGGAGCACCTGG+TGG 0.685211 5_3:-66276235 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTAAGCAGAAGAAGATTAA+AGG - chr5_3:66276127-66276146 None:intergenic 25.0%
ATCTTCTTCTGCTTAATTCT+TGG + chr5_3:66276130-66276149 Msa0826130:CDS 30.0%
! TTCTGCTTAATTCTTGGTTT+TGG + chr5_3:66276136-66276155 Msa0826130:CDS 30.0%
!!! GGTTTTGGTTGTTACTTCTT+TGG + chr5_3:66276151-66276170 Msa0826130:CDS 35.0%
TACACAAACCCTCAAGTTTA+TGG + chr5_3:66276199-66276218 Msa0826130:CDS 35.0%
TTGGCATACCATAAACTTGA+GGG - chr5_3:66276210-66276229 None:intergenic 35.0%
ATTGGCATACCATAAACTTG+AGG - chr5_3:66276211-66276230 None:intergenic 35.0%
!! TTTGGTTTGGAAAATTGTGG+AGG - chr5_3:66276283-66276302 None:intergenic 35.0%
!! AGGTTTGGTTTGGAAAATTG+TGG - chr5_3:66276286-66276305 None:intergenic 35.0%
! TACTTCTTTGGAAGAGCTAG+AGG + chr5_3:66276163-66276182 Msa0826130:CDS 40.0%
GAAGGGAAGGTAGTATTTGC+AGG - chr5_3:66276250-66276269 None:intergenic 45.0%
GGTTTGGAAAATTGTGGAGG+AGG - chr5_3:66276280-66276299 None:intergenic 45.0%
! GTTGGAAGCAAGGTTTGGTT+TGG - chr5_3:66276296-66276315 None:intergenic 45.0%
CAAACGTTGGAAGCAAGGTT+TGG - chr5_3:66276301-66276320 None:intergenic 45.0%
!! GTAGTATTTGCAGGAGCACC+TGG - chr5_3:66276241-66276260 None:intergenic 50.0%
AGGAGGAGATGATGAAGGGA+AGG - chr5_3:66276263-66276282 None:intergenic 50.0%
GTGGAGGAGGAGATGATGAA+GGG - chr5_3:66276267-66276286 None:intergenic 50.0%
TGTGGAGGAGGAGATGATGA+AGG - chr5_3:66276268-66276287 None:intergenic 50.0%
GGTATGCCAATTCCACCACC+AGG + chr5_3:66276220-66276239 Msa0826130:CDS 55.0%
!! GTATTTGCAGGAGCACCTGG+TGG - chr5_3:66276238-66276257 None:intergenic 55.0%
!! GGAGCACCTGGTGGTGGAAT+TGG - chr5_3:66276229-66276248 None:intergenic 60.0%
!! TTTGCAGGAGCACCTGGTGG+TGG - chr5_3:66276235-66276254 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 66276097 66276321 66276097 ID=Msa0826130;Name=Msa0826130
chr5_3 mRNA 66276097 66276321 66276097 ID=Msa0826130-mRNA-1;Parent=Msa0826130;Name=Msa0826130-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|74
chr5_3 exon 66276097 66276321 66276097 ID=Msa0826130-mRNA-1:exon:20795;Parent=Msa0826130-mRNA-1
chr5_3 CDS 66276097 66276321 66276097 ID=Msa0826130-mRNA-1:cds;Parent=Msa0826130-mRNA-1
Gene Sequence

>Msa0826130

ATGGGAATGGTAGTGGTGATATCTCTTCCTTTAATCTTCTTCTGCTTAATTCTTGGTTTTGGTTGTTACTTCTTTGGAAGAGCTAGAGGAAGACGTGATGTTTACACAAACCCTCAAGTTTATGGTATGCCAATTCCACCACCAGGTGCTCCTGCAAATACTACCTTCCCTTCATCATCTCCTCCTCCACAATTTTCCAAACCAAACCTTGCTTCCAACGTTTGA

Protein sequence

>Msa0826130

MGMVVVISLPLIFFCLILGFGCYFFGRARGRRDVYTNPQVYGMPIPPPGAPANTTFPSSSPPPQFSKPNLASNV*