Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0826130 | GAU36262.1 | 81.333 | 75 | 11 | 2 | 1 | 74 | 1 | 73 | 8.23e-31 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0826130 | A0A2Z6NI28 | 81.333 | 75 | 11 | 2 | 1 | 74 | 1 | 73 | 3.93e-31 | 113 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003700 | Msa0826130 | 0.823170 | 1.599972e-53 | -8.615850e-47 |
| Msa0005890 | Msa0826130 | 0.803364 | 3.611867e-49 | -8.615850e-47 |
| Msa0017510 | Msa0826130 | 0.836023 | 1.193244e-56 | -8.615850e-47 |
| Msa0018000 | Msa0826130 | 0.806954 | 6.399492e-50 | -8.615850e-47 |
| Msa0028420 | Msa0826130 | 0.830545 | 2.765310e-55 | -8.615850e-47 |
| Msa0052680 | Msa0826130 | 0.821182 | 4.626798e-53 | -8.615850e-47 |
| Msa0052730 | Msa0826130 | 0.801817 | 7.527119e-49 | -8.615850e-47 |
| Msa0053780 | Msa0826130 | 0.823510 | 1.332788e-53 | -8.615850e-47 |
| Msa0061340 | Msa0826130 | 0.800089 | 1.697308e-48 | -8.615850e-47 |
| Msa0069330 | Msa0826130 | 0.814463 | 1.521179e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0826130 | MtrunA17_Chr5g0448181 | 89.333 | 75 | 7 | 1 | 1 | 74 | 1 | 75 | 7.06e-32 | 105 |
| Msa0826130 | MtrunA17_Chr3g0078121 | 88.000 | 75 | 8 | 1 | 1 | 74 | 1 | 75 | 4.87e-31 | 103 |
| Msa0826130 | MtrunA17_Chr3g0078141 | 74.545 | 55 | 13 | 1 | 4 | 57 | 1 | 55 | 1.21e-23 | 84.3 |
| Msa0826130 | MtrunA17_Chr5g0448161 | 74.545 | 55 | 13 | 1 | 4 | 57 | 1 | 55 | 1.21e-23 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0826130 | AT1G31335.1 | 80.488 | 41 | 8 | 0 | 1 | 41 | 1 | 41 | 2.31e-20 | 76.6 |
Find 25 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTAAGATGGGAATGGTAG+TGG | 0.531670 | 5_3:+66276090 | None:intergenic |
| AGAGAAAATTAAGATGGGAA+TGG | 0.566014 | 5_3:+66276084 | None:intergenic |
| AGGAGGAGATGATGAAGGGA+AGG | 0.519302 | 5_3:-66276260 | None:intergenic |
| AGGTTTGGTTTGGAAAATTG+TGG | 0.482075 | 5_3:-66276283 | None:intergenic |
| ATCTTCTTCTGCTTAATTCT+TGG | 0.163666 | 5_3:+66276130 | Msa0826130:CDS |
| ATTAAGCAGAAGAAGATTAA+AGG | 0.258762 | 5_3:-66276124 | None:intergenic |
| ATTGGCATACCATAAACTTG+AGG | 0.668164 | 5_3:-66276208 | None:intergenic |
| CAAACGTTGGAAGCAAGGTT+TGG | 0.414160 | 5_3:-66276298 | None:intergenic |
| GAACTCAAACGTTGGAAGCA+AGG | 0.528191 | 5_3:-66276303 | None:intergenic |
| GAAGGGAAGGTAGTATTTGC+AGG | 0.442054 | 5_3:-66276247 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCTTCTTCTGCTTAATTCT+TGG | + | chr5_3:66276130-66276149 | Msa0826130:CDS | 30.0% | |
| TACACAAACCCTCAAGTTTA+TGG | + | chr5_3:66276199-66276218 | Msa0826130:CDS | 35.0% | |
| TTGGCATACCATAAACTTGA+GGG | - | chr5_3:66276210-66276229 | None:intergenic | 35.0% | |
| ATTGGCATACCATAAACTTG+AGG | - | chr5_3:66276211-66276230 | None:intergenic | 35.0% | |
| GAAGGGAAGGTAGTATTTGC+AGG | - | chr5_3:66276250-66276269 | None:intergenic | 45.0% | |
| GGTTTGGAAAATTGTGGAGG+AGG | - | chr5_3:66276280-66276299 | None:intergenic | 45.0% | |
| CAAACGTTGGAAGCAAGGTT+TGG | - | chr5_3:66276301-66276320 | None:intergenic | 45.0% | |
| AGGAGGAGATGATGAAGGGA+AGG | - | chr5_3:66276263-66276282 | None:intergenic | 50.0% | |
| GTGGAGGAGGAGATGATGAA+GGG | - | chr5_3:66276267-66276286 | None:intergenic | 50.0% | |
| TGTGGAGGAGGAGATGATGA+AGG | - | chr5_3:66276268-66276287 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 66276097 | 66276321 | 66276097 | ID=Msa0826130;Name=Msa0826130 |
| chr5_3 | mRNA | 66276097 | 66276321 | 66276097 | ID=Msa0826130-mRNA-1;Parent=Msa0826130;Name=Msa0826130-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|74 |
| chr5_3 | exon | 66276097 | 66276321 | 66276097 | ID=Msa0826130-mRNA-1:exon:20795;Parent=Msa0826130-mRNA-1 |
| chr5_3 | CDS | 66276097 | 66276321 | 66276097 | ID=Msa0826130-mRNA-1:cds;Parent=Msa0826130-mRNA-1 |
| Gene Sequence |
>Msa0826130 ATGGGAATGGTAGTGGTGATATCTCTTCCTTTAATCTTCTTCTGCTTAATTCTTGGTTTTGGTTGTTACTTCTTTGGAAGAGCTAGAGGAAGACGTGATGTTTACACAAACCCTCAAGTTTATGGTATGCCAATTCCACCACCAGGTGCTCCTGCAAATACTACCTTCCCTTCATCATCTCCTCCTCCACAATTTTCCAAACCAAACCTTGCTTCCAACGTTTGA |
| Protein sequence |
>Msa0826130 MGMVVVISLPLIFFCLILGFGCYFFGRARGRRDVYTNPQVYGMPIPPPGAPANTTFPSSSPPPQFSKPNLASNV* |
