AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999300 MtrunA17_Chr6g0485481 53.061 49 22 1 3 50 18 66 6.63e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATGAAAACATTGATTAT+TGG 0.176776 6_4:+81211565 Msa0999300:CDS
CATGGGTGGAATTGGAAAAT+AGG 0.301614 6_4:+81210324 Msa0999300:five_prime_UTR
TCAATTGAGATGGTTATGTT+AGG 0.316257 6_4:-81209963 None:intergenic
GCCTTTGGCATGGGTGGAAT+TGG 0.327672 6_4:+81210316 Msa0999300:five_prime_UTR
CAATTGAGATGGTTATGTTA+GGG 0.394866 6_4:-81209962 None:intergenic
CTGAAGTGTGGAGCTGCCTT+TGG 0.398857 6_4:+81210301 Msa0999300:five_prime_UTR
ACCTACACTCAACACATATT+CGG 0.429644 6_4:+81211500 Msa0999300:five_prime_UTR
AGAAAAGGAGTGTTTGTTGT+TGG 0.434642 6_4:-81209933 None:intergenic
AGTATCACCTGACTTTGATA+TGG 0.441071 6_4:-81211664 None:intergenic
GTGTGGAGCTGCCTTTGGCA+TGG 0.496523 6_4:+81210306 Msa0999300:five_prime_UTR
GGAAGAACAACACTCAAAGC+TGG 0.500377 6_4:-81209912 None:intergenic
TGTGGAGCTGCCTTTGGCAT+GGG 0.502829 6_4:+81210307 Msa0999300:five_prime_UTR
TTAATTACCATATCAAAGTC+AGG 0.511911 6_4:+81211657 Msa0999300:CDS
ATGTTAGGGGTGAAAAGAAA+AGG 0.530276 6_4:-81209948 None:intergenic
GGAGCTGCCTTTGGCATGGG+TGG 0.538586 6_4:+81210310 Msa0999300:five_prime_UTR
AATTGAGATGGTTATGTTAG+GGG 0.567185 6_4:-81209961 None:intergenic
CAGCGTGAGGTTCATGCACA+TGG 0.567686 6_4:-81210281 None:intergenic
TCCAATTCCACCCATGCCAA+AGG 0.578726 6_4:-81210317 None:intergenic
GAAGAACAACACTCAAAGCT+GGG 0.590836 6_4:-81209911 None:intergenic
CCAGCATCAATAAGAAAACC+AGG 0.591332 6_4:-81211609 None:intergenic
TGGCAAGACAGAAACGATGA+AGG 0.611685 6_4:+81211585 Msa0999300:CDS
GACAGAAACGATGAAGGACC+TGG 0.614083 6_4:+81211591 Msa0999300:CDS
TCCGAATATGTGTTGAGTGT+AGG 0.619739 6_4:-81211501 None:intergenic
CAGCTCCACACTTCAGCGTG+AGG 0.630034 6_4:-81210294 None:intergenic
ACACTCAACACATATTCGGA+TGG 0.639597 6_4:+81211504 Msa0999300:five_prime_UTR
ATGAACCTCACGCTGAAGTG+TGG 0.657677 6_4:+81210289 Msa0999300:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATAGTTAATAATTTGTA+AGG - chr6_4:81211214-81211233 None:intergenic 10.0%
!! TTACAAATAAAATAGAAAAA+TGG - chr6_4:81211390-81211409 None:intergenic 10.0%
!!! TGTATTTTATCAAGAAATTA+AGG + chr6_4:81210041-81210060 Msa0999300:five_prime_UTR 15.0%
!! AAAATACATCTAAGAATATA+TGG - chr6_4:81210083-81210102 None:intergenic 15.0%
!! ATGATATGCAATAAAATAAA+TGG - chr6_4:81211306-81211325 None:intergenic 15.0%
!! ACATGAAAATTATCAAAAAA+AGG + chr6_4:81211707-81211726 Msa0999300:intron 15.0%
!! CATGAAAATTATCAAAAAAA+GGG + chr6_4:81211708-81211727 Msa0999300:intron 15.0%
!!! TTTTCTTGTTTATTTTTGTT+TGG + chr6_4:81212466-81212485 Msa0999300:CDS 15.0%
!!! GTTTTTAATCACTATGTATT+TGG - chr6_4:81210007-81210026 None:intergenic 20.0%
!!! AAAATACATGCTTTTTTTCT+TGG - chr6_4:81210029-81210048 None:intergenic 20.0%
!!! TTATTATTTTGTAGTCCTTT+TGG + chr6_4:81210145-81210164 Msa0999300:intron 20.0%
!!! ATGCTTTTAATTTTAAACGT+GGG - chr6_4:81210188-81210207 None:intergenic 20.0%
!!! TATGCTTTTAATTTTAAACG+TGG - chr6_4:81210189-81210208 None:intergenic 20.0%
!! ATATTTGTTTATGTTGTGTT+TGG + chr6_4:81210785-81210804 Msa0999300:intron 20.0%
!!! TAAGTTTTCGAAATTATAAG+TGG + chr6_4:81210808-81210827 Msa0999300:intron 20.0%
!! CTAAAAATGATAATATCATG+AGG - chr6_4:81210946-81210965 None:intergenic 20.0%
!! CCTCAAATATGAATTATATT+AGG - chr6_4:81211088-81211107 None:intergenic 20.0%
!! CCTAATATAATTCATATTTG+AGG + chr6_4:81211085-81211104 Msa0999300:intron 20.0%
!! ATATGCAATAAAATAAATGG+CGG - chr6_4:81211303-81211322 None:intergenic 20.0%
!!! AAGATGAAAACATTGATTAT+TGG + chr6_4:81211565-81211584 Msa0999300:CDS 20.0%
!!! TTAAAAAGTGTTAGACTAAA+TGG + chr6_4:81211883-81211902 Msa0999300:intron 20.0%
!! CATGATTATCATACATTATT+CGG - chr6_4:81212116-81212135 None:intergenic 20.0%
!!! AAAATAGAATATTTTGTCTC+AGG - chr6_4:81212175-81212194 None:intergenic 20.0%
!! TTTTAAACGTGGGAAAAAAA+TGG - chr6_4:81210178-81210197 None:intergenic 25.0%
! ATTAACTAATAAAAAAGGGC+TGG - chr6_4:81210446-81210465 None:intergenic 25.0%
!!! TTTTTAAGTAAAAAAAGGGC+TGG - chr6_4:81210496-81210515 None:intergenic 25.0%
! ATGATAGAAACATAACACAA+TGG - chr6_4:81210710-81210729 None:intergenic 25.0%
!!! GCGGTTTGTTAAAATAATTA+AGG - chr6_4:81210735-81210754 None:intergenic 25.0%
! CCAATAAAAAATGTTACTAC+CGG + chr6_4:81210849-81210868 Msa0999300:intron 25.0%
! CAATAAAAAATGTTACTACC+GGG + chr6_4:81210850-81210869 Msa0999300:intron 25.0%
!!! TTATTGATAGTGAAAATCGT+AGG + chr6_4:81210984-81211003 Msa0999300:intron 25.0%
! TTAATTACCATATCAAAGTC+AGG + chr6_4:81211657-81211676 Msa0999300:CDS 25.0%
!!! ACTTTACTTTTTTACTTCAG+TGG + chr6_4:81211684-81211703 Msa0999300:intron 25.0%
!! ACCAATGAAACTCTTTTATA+CGG + chr6_4:81211749-81211768 Msa0999300:intron 25.0%
! TTCATGATTGATGCTTATTT+CGG + chr6_4:81211926-81211945 Msa0999300:intron 25.0%
! CGAATAATGTATGATAATCA+TGG + chr6_4:81212114-81212133 Msa0999300:intron 25.0%
AATTGAGATGGTTATGTTAG+GGG - chr6_4:81209964-81209983 None:intergenic 30.0%
CAATTGAGATGGTTATGTTA+GGG - chr6_4:81209965-81209984 None:intergenic 30.0%
TCAATTGAGATGGTTATGTT+AGG - chr6_4:81209966-81209985 None:intergenic 30.0%
! GAGAAAGTTTTCAATTGAGA+TGG - chr6_4:81209976-81209995 None:intergenic 30.0%
CAACAAGACAAATCATGTTT+AGG - chr6_4:81210248-81210267 None:intergenic 30.0%
! CTAAACATGATTTGTCTTGT+TGG + chr6_4:81210246-81210265 Msa0999300:intron 30.0%
AAACATAAGATGATGAAGAG+AGG + chr6_4:81210573-81210592 Msa0999300:intron 30.0%
!!! CCGGTAGTAACATTTTTTAT+TGG - chr6_4:81210852-81210871 None:intergenic 30.0%
ACTATTAAAGATCTCTCTGT+AGG - chr6_4:81211462-81211481 None:intergenic 30.0%
ACCGTATAAAAGAGTTTCAT+TGG - chr6_4:81211753-81211772 None:intergenic 30.0%
AATTCATCATGCGTTATTTG+AGG - chr6_4:81212081-81212100 None:intergenic 30.0%
ATAGAATCGAATTAAGAGAG+TGG + chr6_4:81212201-81212220 Msa0999300:intron 30.0%
TAGAATCGAATTAAGAGAGT+GGG + chr6_4:81212202-81212221 Msa0999300:intron 30.0%
ATAATATCATGTAGCGCTAA+AGG + chr6_4:81212324-81212343 Msa0999300:intron 30.0%
TGGTGAATTTCATTCATCAA+TGG + chr6_4:81212386-81212405 Msa0999300:intron 30.0%
! TTTGTTTGGTCTGTTTTATG+TGG + chr6_4:81212480-81212499 Msa0999300:CDS 30.0%
!! AGAAAAGGAGTGTTTGTTGT+TGG - chr6_4:81209936-81209955 None:intergenic 35.0%
ATGTTAGGGGTGAAAAGAAA+AGG - chr6_4:81209951-81209970 None:intergenic 35.0%
AAAAATGGAAAGGGACCAAA+AGG - chr6_4:81210163-81210182 None:intergenic 35.0%
ACGTGGGAAAAAAATGGAAA+GGG - chr6_4:81210172-81210191 None:intergenic 35.0%
AACGTGGGAAAAAAATGGAA+AGG - chr6_4:81210173-81210192 None:intergenic 35.0%
ACACATCCGACAAATCTATA+CGG - chr6_4:81210664-81210683 None:intergenic 35.0%
ATACACAACTATTGTGAGAG+TGG - chr6_4:81210757-81210776 None:intergenic 35.0%
ATTCATTACCTAGAAGAACC+CGG - chr6_4:81210871-81210890 None:intergenic 35.0%
ACCTACACTCAACACATATT+CGG + chr6_4:81211500-81211519 Msa0999300:five_prime_UTR 35.0%
AGTATCACCTGACTTTGATA+TGG - chr6_4:81211667-81211686 None:intergenic 35.0%
! GAGTTTCATTGGTTGCAATA+AGG - chr6_4:81211742-81211761 None:intergenic 35.0%
TATTAGCATGAGATGTGTTG+AGG - chr6_4:81211781-81211800 None:intergenic 35.0%
TATTTGTGACTCTCGATGTA+TGG + chr6_4:81211812-81211831 Msa0999300:intron 35.0%
!! AATTTGTTACGTGACTTTGC+AGG + chr6_4:81211851-81211870 Msa0999300:intron 35.0%
ATGATTGATGCTTATTTCGG+AGG + chr6_4:81211929-81211948 Msa0999300:intron 35.0%
TGATTGATGCTTATTTCGGA+GGG + chr6_4:81211930-81211949 Msa0999300:intron 35.0%
ATGTGAACAACACAACTTTG+TGG - chr6_4:81211975-81211994 None:intergenic 35.0%
AATTGTCTCTTCATCCTCTT+TGG - chr6_4:81212001-81212020 None:intergenic 35.0%
AAAGAGGATGAAGAGACAAT+TGG + chr6_4:81212000-81212019 Msa0999300:intron 35.0%
AGAGACAATTGGAAGTTCTT+TGG + chr6_4:81212011-81212030 Msa0999300:intron 35.0%
GAGACAATTGGAAGTTCTTT+GGG + chr6_4:81212012-81212031 Msa0999300:intron 35.0%
AACACAATCAACAAACGCAT+TGG - chr6_4:81212049-81212068 None:intergenic 35.0%
CATAAAAGTCAGGTAGTGAT+TGG - chr6_4:81212147-81212166 None:intergenic 35.0%
! AGAATATTTTGTCTCAGGAG+TGG - chr6_4:81212170-81212189 None:intergenic 35.0%
! TATGCATCTAGCTTCTTCAT+TGG - chr6_4:81212246-81212265 None:intergenic 35.0%
AATTCACCAAGAAGAGTAGA+AGG - chr6_4:81212375-81212394 None:intergenic 35.0%
CATGGGTGGAATTGGAAAAT+AGG + chr6_4:81210324-81210343 Msa0999300:five_prime_UTR 40.0%
! TGAGTTGAATCTACTCACCA+CGG + chr6_4:81210618-81210637 Msa0999300:intron 40.0%
TCGATGCCGTATAGATTTGT+CGG + chr6_4:81210655-81210674 Msa0999300:intron 40.0%
TCCGAATATGTGTTGAGTGT+AGG - chr6_4:81211504-81211523 None:intergenic 40.0%
ACACTCAACACATATTCGGA+TGG + chr6_4:81211504-81211523 Msa0999300:five_prime_UTR 40.0%
CCAGCATCAATAAGAAAACC+AGG - chr6_4:81211612-81211631 None:intergenic 40.0%
!! CCTGGTTTTCTTATTGATGC+TGG + chr6_4:81211609-81211628 Msa0999300:CDS 40.0%
GTGTTAGACTAAATGGCTGA+CGG + chr6_4:81211890-81211909 Msa0999300:intron 40.0%
GATTGATGCTTATTTCGGAG+GGG + chr6_4:81211931-81211950 Msa0999300:intron 40.0%
ATTGATGCTTATTTCGGAGG+GGG + chr6_4:81211932-81211951 Msa0999300:intron 40.0%
GAACAACACAACTTTGTGGT+GGG - chr6_4:81211971-81211990 None:intergenic 40.0%
TGAACAACACAACTTTGTGG+TGG - chr6_4:81211972-81211991 None:intergenic 40.0%
GTTGTTCACATCTTCCAAAG+AGG + chr6_4:81211984-81212003 Msa0999300:intron 40.0%
! CTGTTTCTCTTAGAGATGCT+AGG + chr6_4:81212274-81212293 Msa0999300:intron 40.0%
CTTAGAGATGCTAGGTCTTT+AGG + chr6_4:81212282-81212301 Msa0999300:intron 40.0%
GGTCTTTCTTCTTCATCTTC+TGG + chr6_4:81212345-81212364 Msa0999300:intron 40.0%
GGAAGAACAACACTCAAAGC+TGG - chr6_4:81209915-81209934 None:intergenic 45.0%
!! CTATACGGCATCGATTCTGA+CGG - chr6_4:81210649-81210668 None:intergenic 45.0%
CACAACTATTGTGAGAGTGG+CGG - chr6_4:81210754-81210773 None:intergenic 45.0%
TGTTACTACCGGGTTCTTCT+AGG + chr6_4:81210860-81210879 Msa0999300:intron 45.0%
TGGCAAGACAGAAACGATGA+AGG + chr6_4:81211585-81211604 Msa0999300:CDS 45.0%
TCAGGAGTGGCATAAAAGTC+AGG - chr6_4:81212157-81212176 None:intergenic 45.0%
GGTTCACCTTCTACTCTTCT+TGG + chr6_4:81212366-81212385 Msa0999300:intron 45.0%
!! ATTTATTAACTAATAAAAAA+GGG - chr6_4:81210450-81210469 None:intergenic 5.0%
!! TATTTATTAACTAATAAAAA+AGG - chr6_4:81210451-81210470 None:intergenic 5.0%
!!! TTATTTTTTAAGTAAAAAAA+GGG - chr6_4:81210500-81210519 None:intergenic 5.0%
!!! ATTATTTTTTAAGTAAAAAA+AGG - chr6_4:81210501-81210520 None:intergenic 5.0%
!! TAAATTATTGTAAAAATAAA+TGG - chr6_4:81210898-81210917 None:intergenic 5.0%
!!! ATATTTTTTTTTTATCTATA+TGG - chr6_4:81211135-81211154 None:intergenic 5.0%
!! TAAAAAAAAAATATATTACA+TGG + chr6_4:81211141-81211160 Msa0999300:intron 5.0%
ATGAACCTCACGCTGAAGTG+TGG + chr6_4:81210289-81210308 Msa0999300:five_prime_UTR 50.0%
TCCAATTCCACCCATGCCAA+AGG - chr6_4:81210320-81210339 None:intergenic 50.0%
GTTGAATCTACTCACCACGG+CGG + chr6_4:81210621-81210640 Msa0999300:intron 50.0%
GACAGAAACGATGAAGGACC+TGG + chr6_4:81211591-81211610 Msa0999300:CDS 50.0%
CAGCGTGAGGTTCATGCACA+TGG - chr6_4:81210284-81210303 None:intergenic 55.0%
CTGAAGTGTGGAGCTGCCTT+TGG + chr6_4:81210301-81210320 Msa0999300:five_prime_UTR 55.0%
TGTGGAGCTGCCTTTGGCAT+GGG + chr6_4:81210307-81210326 Msa0999300:five_prime_UTR 55.0%
GCCTTTGGCATGGGTGGAAT+TGG + chr6_4:81210316-81210335 Msa0999300:five_prime_UTR 55.0%
CAGCTCCACACTTCAGCGTG+AGG - chr6_4:81210297-81210316 None:intergenic 60.0%
GTGTGGAGCTGCCTTTGGCA+TGG + chr6_4:81210306-81210325 Msa0999300:five_prime_UTR 60.0%
GGAGCTGCCTTTGGCATGGG+TGG + chr6_4:81210310-81210329 Msa0999300:five_prime_UTR 65.0%
CGATTCTGACGGTACCGCCG+TGG - chr6_4:81210638-81210657 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81209912 81212506 81209912 ID=Msa0999300;Name=Msa0999300
chr6_4 mRNA 81209912 81212506 81209912 ID=Msa0999300-mRNA-1;Parent=Msa0999300;Name=Msa0999300-mRNA-1;_AED=0.22;_eAED=0.32;_QI=306|0.33|0.5|1|0|0|4|0|58
chr6_4 exon 81209912 81210062 81209912 ID=Msa0999300-mRNA-1:exon:15527;Parent=Msa0999300-mRNA-1
chr6_4 exon 81210274 81210345 81210274 ID=Msa0999300-mRNA-1:exon:15528;Parent=Msa0999300-mRNA-1
chr6_4 exon 81211466 81211678 81211466 ID=Msa0999300-mRNA-1:exon:15529;Parent=Msa0999300-mRNA-1
chr6_4 exon 81212463 81212506 81212463 ID=Msa0999300-mRNA-1:exon:15530;Parent=Msa0999300-mRNA-1
chr6_4 five_prime_UTR 81209912 81210062 81209912 ID=Msa0999300-mRNA-1:five_prime_utr;Parent=Msa0999300-mRNA-1
chr6_4 five_prime_UTR 81210274 81210345 81210274 ID=Msa0999300-mRNA-1:five_prime_utr;Parent=Msa0999300-mRNA-1
chr6_4 five_prime_UTR 81211466 81211548 81211466 ID=Msa0999300-mRNA-1:five_prime_utr;Parent=Msa0999300-mRNA-1
chr6_4 CDS 81211549 81211678 81211549 ID=Msa0999300-mRNA-1:cds;Parent=Msa0999300-mRNA-1
chr6_4 CDS 81212463 81212506 81212463 ID=Msa0999300-mRNA-1:cds;Parent=Msa0999300-mRNA-1
Gene Sequence

>Msa0999300

ATGAAATATAAATTTAAAGATGAAAACATTGATTATTGGCAAGACAGAAACGATGAAGGACCTGGTTTTCTTATTGATGCTGGCAAAATACATAATAGATTATGTGAGTTAATTACCATATCAAAGTCAGAGCTTTTCTTGTTTATTTTTGTTTGGTCTGTTTTATGTGGTAGC

Protein sequence

>Msa0999300

MKYKFKDENIDYWQDRNDEGPGFLIDAGKIHNRLCELITISKSELFLFIFVWSVLCGS