AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0832760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0832760 MtrunA17_Chr5g0397981 97.203 286 5 1 1 286 19 301 0.0 580
Msa0832760 MtrunA17_Chr4g0057281 76.208 269 61 2 20 286 19 286 5.27e-158 441
Msa0832760 MtrunA17_Chr8g0350401 56.401 289 117 3 1 281 1 288 4.03e-119 343
Msa0832760 MtrunA17_Chr7g0265551 56.314 293 112 5 1 280 73 362 3.38e-112 328
Msa0832760 MtrunA17_Chr1g0203751 57.308 260 109 2 20 278 28 286 7.06e-112 325
Msa0832760 MtrunA17_Chr6g0472801 55.344 262 115 2 16 276 18 278 1.48e-110 321
Msa0832760 MtrunA17_Chr7g0272101 24.453 274 173 8 23 278 4 261 7.69e-28 108
Msa0832760 MtrunA17_Chr4g0010881 25.379 264 161 8 23 277 4 240 3.70e-24 97.8
Msa0832760 MtrunA17_Chr1g0189671 34.722 144 91 3 22 164 6 147 4.05e-22 93.2
Msa0832760 MtrunA17_Chr1g0189611 34.932 146 91 3 22 164 6 150 1.69e-21 91.3
Msa0832760 MtrunA17_Chr1g0189591 33.117 154 96 4 13 161 6 157 4.82e-21 90.9
Msa0832760 MtrunA17_Chr1g0189641 33.562 146 90 4 22 164 6 147 3.90e-19 85.1
Msa0832760 MtrunA17_Chr1g0189751 32.667 150 94 4 18 164 2 147 9.51e-19 84.3
Msa0832760 MtrunA17_Chr1g0189721 25.940 266 184 6 18 273 2 264 1.67e-18 83.2
Msa0832760 MtrunA17_Chr7g0272091 22.414 290 185 9 15 273 2 282 1.95e-18 83.2
Msa0832760 MtrunA17_Chr1g0189621 26.236 263 178 9 22 273 6 263 2.76e-18 82.4
Msa0832760 MtrunA17_Chr1g0189821 25.725 276 169 10 22 275 6 267 1.33e-17 80.5
Msa0832760 MtrunA17_Chr1g0203281 31.250 144 96 3 22 164 6 147 1.58e-17 80.5
Msa0832760 MtrunA17_Chr1g0190071 31.973 147 95 3 22 164 6 151 3.68e-17 79.7
Msa0832760 MtrunA17_Chr1g0192371 28.049 164 112 4 18 176 6 168 4.56e-16 76.6
Msa0832760 MtrunA17_Chr1g0189781 29.870 154 97 4 18 164 6 155 7.86e-16 75.9
Msa0832760 MtrunA17_Chr1g0189771 28.571 161 102 4 12 164 4 159 3.86e-15 72.0
Msa0832760 MtrunA17_Chr3g0097191 31.944 144 91 4 20 161 10 148 1.13e-14 72.4
Msa0832760 MtrunA17_Chr1g0203331 31.008 129 86 3 37 164 6 132 4.10e-14 70.5
Msa0832760 MtrunA17_Chr1g0189661 30.872 149 93 5 22 164 6 150 1.45e-13 69.7
Msa0832760 MtrunA17_Chr1g0189711 28.431 204 120 7 22 216 6 192 6.76e-13 67.4
Msa0832760 MtrunA17_Chr3g0095261 31.852 135 83 5 20 152 10 137 4.96e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0832760 AT1G12710.1 67.181 259 83 2 20 277 28 285 6.19e-128 365
Msa0832760 AT1G12710.2 57.807 301 83 3 20 277 28 327 7.00e-119 344
Msa0832760 AT3G61060.1 58.242 273 111 3 9 279 14 285 6.18e-118 340
Msa0832760 AT3G61060.2 58.029 274 111 4 9 279 14 286 2.18e-116 336
Msa0832760 AT1G63090.1 59.507 284 107 3 1 277 1 283 1.56e-114 332
Msa0832760 AT3G53000.1 53.333 285 120 3 1 273 1 284 1.13e-103 304
Msa0832760 AT5G52120.1 48.772 285 137 3 1 277 1 284 5.14e-98 290
Msa0832760 AT2G02360.1 34.591 159 94 4 9 164 3 154 9.10e-27 105
Msa0832760 AT2G02230.1 31.792 173 108 6 17 184 29 196 1.35e-23 98.6
Msa0832760 AT2G02240.1 34.752 141 86 3 23 162 64 199 1.65e-22 95.5
Msa0832760 AT1G09155.1 24.823 282 184 7 23 277 3 283 3.94e-22 94.0
Msa0832760 AT2G02340.1 32.927 164 100 5 18 176 34 192 4.84e-22 94.0
Msa0832760 AT2G02350.2 36.054 147 83 5 16 158 20 159 9.21e-22 92.8
Msa0832760 AT2G02250.1 30.667 150 95 3 15 161 42 185 1.75e-21 92.4
Msa0832760 AT2G02320.1 31.285 179 110 6 3 176 22 192 4.12e-20 88.6
Msa0832760 AT5G24560.1 26.347 167 112 4 22 187 5 161 1.48e-18 82.0
Msa0832760 AT2G02310.1 31.544 149 92 4 18 163 43 184 1.69e-18 84.0
Msa0832760 AT1G56250.1 24.232 293 164 8 23 273 4 280 9.79e-17 79.0
Msa0832760 AT1G56240.1 24.028 283 185 5 23 277 4 284 3.03e-16 77.4
Msa0832760 AT1G80110.1 24.242 264 181 9 20 273 1 255 5.04e-16 76.3
Msa0832760 AT2G02300.1 26.891 238 141 10 16 242 30 245 6.98e-13 67.8

Find 106 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATTTACCAATCAATT+TGG 0.149392 5_4:+5853420 Msa0832760:CDS
AATTTCTTCTGTTAATTAAA+TGG 0.179440 5_4:+5855950 Msa0832760:three_prime_UTR
TGGTAAATCATGAAAGATTT+TGG 0.209100 5_4:-5853410 None:intergenic
AAACTGCTCCTAACCATTTC+AGG 0.215027 5_4:+5855782 Msa0832760:exon
ATCTCAATCTCAATCTTCTA+TGG 0.226201 5_4:+5853174 Msa0832760:exon
ATTGAAGCGACGCAACTTTC+AGG 0.233304 5_4:-5853262 None:intergenic
CTTCTTCTGCTGATTTCGTT+TGG 0.259520 5_4:+5853350 Msa0832760:CDS
GGGTGCAGAAAGATTGAAAA+AGG 0.264780 5_4:-5853123 None:intergenic
GGTTCAACCGGTTCAACTTT+AGG 0.271212 5_4:+5853232 Msa0832760:CDS
TAAACTTCAGATATACTCTT+AGG 0.292898 5_4:-5856072 None:intergenic
TTGAAATTGAATGGTAATAA+TGG 0.311701 5_4:-5853152 None:intergenic
CTGTATGTCCCGGCTGGAAA+TGG 0.317696 5_4:-5855429 None:intergenic
TTCTTCTGCTGATTTCGTTT+GGG 0.325116 5_4:+5853351 Msa0832760:CDS
CACTAAAGGTGGTCTGTGTT+TGG 0.348141 5_4:+5855710 Msa0832760:CDS
CATGATTTACCAATCAATTT+GGG 0.351067 5_4:+5853421 Msa0832760:CDS
ACATCAGACACAAATGTATA+TGG 0.354865 5_4:-5856143 None:intergenic
TTTGTTGTGTGCAGAAAGTT+TGG 0.359355 5_4:+5854163 Msa0832760:intron
AGCCTGTGCGATTCCAACTT+TGG 0.369551 5_4:+5855541 Msa0832760:CDS
GTGGACTCGGTTTGGTATGA+TGG 0.370474 5_4:-5853039 None:intergenic
CGCTTCAATCATCGGTTACA+TGG 0.370713 5_4:+5853276 Msa0832760:CDS
TTCTGAAAGGAACTGGCAAT+TGG 0.389942 5_4:+5855598 Msa0832760:CDS
CGGTACTATCCTATCTGATT+AGG 0.390805 5_4:-5852972 None:intergenic
TCTTTCTACCTGAAATGGTT+AGG 0.406400 5_4:-5855790 None:intergenic
TTATTCTTCAGAATACATCT+AGG 0.422202 5_4:+5855453 Msa0832760:CDS
TTGCGGAAGATTTGAATGTA+TGG 0.422974 5_4:+5856028 Msa0832760:three_prime_UTR
CCGGTTGTCCTTGGTGGACT+CGG 0.423403 5_4:-5853052 None:intergenic
TGTCCTTGGTGGACTCGGTT+TGG 0.428062 5_4:-5853047 None:intergenic
TCCAACCAAACCGGTTCAAC+CGG 0.434266 5_4:+5853220 Msa0832760:CDS
TAAAGTTGAACCGGTTGAAC+CGG 0.438212 5_4:-5853230 None:intergenic
ACGACGTCCAAACCTCTTGT+AGG 0.439552 5_4:-5855481 None:intergenic
GGGCAAGCCTACAAGAGGTT+TGG 0.441598 5_4:+5855474 Msa0832760:CDS
GAATTGATGATAGAAGATAT+TGG 0.446914 5_4:+5854250 Msa0832760:CDS
CGACTTAATACTATTGATGA+TGG 0.447327 5_4:+5853472 Msa0832760:CDS
TTGAACCGGTTGAACCGGTT+TGG 0.448850 5_4:-5853225 None:intergenic
ACCGCTGAGTTGGCATTTGT+CGG 0.450818 5_4:+5852991 Msa0832760:five_prime_UTR
AGTCATAGAGAATTTGACTT+TGG 0.459733 5_4:-5855670 None:intergenic
CCGAGTCCACCAAGGACAAC+CGG 0.461694 5_4:+5853052 Msa0832760:five_prime_UTR
TTTGAGTACAGTAGAATGAA+TGG 0.461971 5_4:+5856165 Msa0832760:three_prime_UTR
ATATCATCACTGAAGAATCT+AGG 0.462039 5_4:+5854277 Msa0832760:CDS
AGCCACGGGCAAGCTTTGCT+TGG 0.464990 5_4:+5854194 Msa0832760:CDS
ACTTTCCGTCCTAATCAGAT+AGG 0.468494 5_4:+5852963 None:intergenic
TTCCAACTTTGGACTTCAGA+TGG 0.477540 5_4:+5855552 Msa0832760:CDS
TAATTCACCTAAAGTTGAAC+CGG 0.483165 5_4:-5853239 None:intergenic
AGTTGCGTCGCTTCAATCAT+CGG 0.485599 5_4:+5853268 Msa0832760:CDS
ACGCGTACACCGGTTGTCCT+TGG 0.487127 5_4:-5853061 None:intergenic
TTGCATACCTTCAGCATACC+TGG 0.490977 5_4:+5855376 Msa0832760:CDS
TGTATGTCCCGGCTGGAAAT+GGG 0.498240 5_4:-5855428 None:intergenic
TCATTTCGATCCAACCAAAC+CGG 0.498725 5_4:+5853211 Msa0832760:CDS
TGCAACACTGAACATGTTCA+TGG 0.501094 5_4:+5855507 Msa0832760:CDS
CCTGGTGGTTTGAAGTGGAC+GGG 0.501663 5_4:+5855394 Msa0832760:CDS
AACAAGGGACTATCAGTACT+TGG 0.501726 5_4:+5854228 Msa0832760:CDS
GGTGATTTCACCGTTGAGGA+TGG 0.503816 5_4:+5855636 Msa0832760:CDS
CTTGGCTATATCAGCTAACA+AGG 0.503821 5_4:+5854212 Msa0832760:CDS
CGTACACCGGTTGTCCTTGG+TGG 0.504972 5_4:-5853058 None:intergenic
ACCGGTTGAACCGGTTTGGT+TGG 0.509700 5_4:-5853221 None:intergenic
AATGGTAATAATGGAAAAGA+GGG 0.510959 5_4:-5853143 None:intergenic
ATTGATTGCACGCACACTAA+AGG 0.512444 5_4:+5855696 Msa0832760:CDS
AGATTGAGATTGAAATTGAA+TGG 0.515418 5_4:-5853161 None:intergenic
GAATGGTAATAATGGAAAAG+AGG 0.517273 5_4:-5853144 None:intergenic
TCTAGTATGTATAGAACATA+GGG 0.518228 5_4:+5855831 Msa0832760:three_prime_UTR
CACTGAACATGTTCATGGGT+GGG 0.526344 5_4:+5855512 Msa0832760:CDS
CAGTTCCTTTCAGAAAACAC+TGG 0.526894 5_4:-5855590 None:intergenic
TCTCAATCTCAATCTTCTAT+GGG 0.534797 5_4:+5853175 Msa0832760:exon
GCCCATCTGAAGTCCAAAGT+TGG 0.536214 5_4:-5855554 None:intergenic
CCCGTCCACTTCAAACCACC+AGG 0.538040 5_4:-5855394 None:intergenic
AATAAGCTGTATGTCCCGGC+TGG 0.538928 5_4:-5855435 None:intergenic
TTGACTTCCCATTTCCAGCC+GGG 0.542798 5_4:+5855421 Msa0832760:CDS
TCCAACTTTGGACTTCAGAT+GGG 0.543088 5_4:+5855553 Msa0832760:CDS
TTGAGTACAGTAGAATGAAT+GGG 0.545617 5_4:+5856166 Msa0832760:three_prime_UTR
GCATACCTGGTGGTTTGAAG+TGG 0.546087 5_4:+5855389 Msa0832760:CDS
AGGAGAAGATGGTAATTTGT+CGG 0.546308 5_4:-5853385 None:intergenic
GTGATTTCACCGTTGAGGAT+GGG 0.546700 5_4:+5855637 Msa0832760:CDS
TATTCTTCAGAATACATCTA+GGG 0.555396 5_4:+5855454 Msa0832760:CDS
TTGAGAAAGTTCTATGCTTG+CGG 0.556205 5_4:+5856011 Msa0832760:three_prime_UTR
ACCTGGTGGTTTGAAGTGGA+CGG 0.561016 5_4:+5855393 Msa0832760:CDS
GTCTAGTATGTATAGAACAT+AGG 0.562493 5_4:+5855830 Msa0832760:three_prime_UTR
GTTTGGATTGATAGAGCCAC+GGG 0.562866 5_4:+5854180 Msa0832760:CDS
ATAGGATAGTACCGCTGAGT+TGG 0.564846 5_4:+5852981 Msa0832760:five_prime_UTR
ATGAATGGGAATAAGAGTGT+TGG 0.570486 5_4:+5856180 Msa0832760:three_prime_UTR
GAAGAATAAGCTGTATGTCC+CGG 0.575375 5_4:-5855439 None:intergenic
GTCCAAAGTTGGAATCGCAC+AGG 0.578305 5_4:-5855543 None:intergenic
TGCAGGTGATTTCACCGTTG+AGG 0.581687 5_4:+5855632 Msa0832760:CDS
TGCGGAAGATTTGAATGTAT+GGG 0.582252 5_4:+5856029 Msa0832760:three_prime_UTR
ATGTACAGCAACGCGTACAC+CGG 0.585734 5_4:-5853071 None:intergenic
CTGGTGGTTTGAAGTGGACG+GGG 0.591797 5_4:+5855395 Msa0832760:CDS
GTTGACTTCCCATTTCCAGC+CGG 0.592053 5_4:+5855420 Msa0832760:CDS
GAACATAGGGCGGTGTCATG+AGG 0.596499 5_4:+5855844 Msa0832760:three_prime_UTR
AGTTCCTTTCAGAAAACACT+GGG 0.601469 5_4:-5855589 None:intergenic
AGTTTGGATTGATAGAGCCA+CGG 0.603796 5_4:+5854179 Msa0832760:CDS
GATTGCACGCACACTAAAGG+TGG 0.628473 5_4:+5855699 Msa0832760:CDS
GCAACACTGAACATGTTCAT+GGG 0.629375 5_4:+5855508 Msa0832760:CDS
TGATGAATTCCCATCCTCAA+CGG 0.629667 5_4:-5855646 None:intergenic
ATCTAGGGCAAGCCTACAAG+AGG 0.631041 5_4:+5855469 Msa0832760:CDS
CAACGGTGAAATCACCTGCA+TGG 0.636855 5_4:-5855629 None:intergenic
TACTATTGATGATGGCACCA+AGG 0.640156 5_4:+5853480 Msa0832760:CDS
AGCCAAGCAAAGCTTGCCCG+TGG 0.641717 5_4:-5854196 None:intergenic
GCAAGTTGACAGATCTGAGG+AGG 0.643594 5_4:-5853301 None:intergenic
ACACTGAACATGTTCATGGG+TGG 0.656933 5_4:+5855511 Msa0832760:CDS
CATACCTTCAGCATACCTGG+TGG 0.660309 5_4:+5855379 Msa0832760:CDS
CAAACCACCAGGTATGCTGA+AGG 0.668408 5_4:-5855383 None:intergenic
TCCTTTCAGAAAACACTGGG+AGG 0.675908 5_4:-5855586 None:intergenic
GTTGCAAGTTGACAGATCTG+AGG 0.684069 5_4:-5853304 None:intergenic
TCCGACAAATGCCAACTCAG+CGG 0.719381 5_4:-5852992 None:intergenic
AGTATGTATAGAACATAGGG+CGG 0.724118 5_4:+5855834 Msa0832760:three_prime_UTR
TTGGCTATATCAGCTAACAA+GGG 0.728498 5_4:+5854213 Msa0832760:CDS
ATACCAAACCGAGTCCACCA+AGG 0.749371 5_4:+5853044 Msa0832760:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTAATTTTGAATGAT+TGG + chr5_4:5853679-5853698 Msa0832760:intron 10.0%
!! ACATTATAATCTATTTAATA+GGG + chr5_4:5854913-5854932 Msa0832760:intron 10.0%
!!! TTATTATAAATCTATTTTAG+TGG + chr5_4:5855310-5855329 Msa0832760:intron 10.0%
!!! TCTATCTTTTTCTATAAAAT+TGG + chr5_4:5854024-5854043 Msa0832760:intron 15.0%
!!! AGTTATGATATGATATTTTT+GGG + chr5_4:5854523-5854542 Msa0832760:intron 15.0%
!!! GTATTACATAATTTTCATTA+AGG + chr5_4:5854707-5854726 Msa0832760:intron 15.0%
!!! TTTTCATTAAGGAATATAAT+TGG + chr5_4:5854718-5854737 Msa0832760:intron 15.0%
!! CACATTATAATCTATTTAAT+AGG + chr5_4:5854912-5854931 Msa0832760:intron 15.0%
!! AATTTCTTCTGTTAATTAAA+TGG + chr5_4:5855950-5855969 Msa0832760:three_prime_UTR 15.0%
!! TTGAAATTGAATGGTAATAA+TGG - chr5_4:5853155-5853174 None:intergenic 20.0%
!!! TATACCTTTAGAATCAATTT+TGG + chr5_4:5853809-5853828 Msa0832760:intron 20.0%
!!! ATTGTATTTTCTAGAATAGA+TGG + chr5_4:5854375-5854394 Msa0832760:intron 20.0%
!!! GAGTTATGATATGATATTTT+TGG + chr5_4:5854522-5854541 Msa0832760:intron 20.0%
!!! TTATTTTAGATGATTTAGAC+TGG + chr5_4:5854602-5854621 Msa0832760:intron 20.0%
!!! GATATTTATTGTTTTTATCG+AGG - chr5_4:5854893-5854912 None:intergenic 20.0%
!! TTACATAGATAAATCTTTGT+CGG + chr5_4:5855157-5855176 Msa0832760:intron 20.0%
!!! GAGTTTTTATATGATTCTTT+AGG + chr5_4:5855878-5855897 Msa0832760:three_prime_UTR 20.0%
!!! TTCTATCAAGTTTTTGTATA+TGG + chr5_4:5855977-5855996 Msa0832760:three_prime_UTR 20.0%
! CATAAGAAAAAGAGAGAAAT+GGG - chr5_4:5853099-5853118 None:intergenic 25.0%
! TCATAAGAAAAAGAGAGAAA+TGG - chr5_4:5853100-5853119 None:intergenic 25.0%
!! AATGGTAATAATGGAAAAGA+GGG - chr5_4:5853146-5853165 None:intergenic 25.0%
! AGATTGAGATTGAAATTGAA+TGG - chr5_4:5853164-5853183 None:intergenic 25.0%
!! TGGTAAATCATGAAAGATTT+TGG - chr5_4:5853413-5853432 None:intergenic 25.0%
! TCATGATTTACCAATCAATT+TGG + chr5_4:5853420-5853439 Msa0832760:CDS 25.0%
! CATGATTTACCAATCAATTT+GGG + chr5_4:5853421-5853440 Msa0832760:CDS 25.0%
! AATCAATAAAGTAATCACCT+TGG - chr5_4:5853500-5853519 None:intergenic 25.0%
!! AAATGTATGTGTGCTTATTT+TGG + chr5_4:5853629-5853648 Msa0832760:intron 25.0%
!! GAGTTTAAAATCAATTCTAG+AGG + chr5_4:5853735-5853754 Msa0832760:intron 25.0%
!!! ATAAGTTTTGTGAAACTGTT+TGG + chr5_4:5853904-5853923 Msa0832760:intron 25.0%
!!! TAAGTTTTGTGAAACTGTTT+GGG + chr5_4:5853905-5853924 Msa0832760:intron 25.0%
!! GAATTGATGATAGAAGATAT+TGG + chr5_4:5854250-5854269 Msa0832760:CDS 25.0%
!!! GGACTGTCTTAATTTTTTTT+TGG + chr5_4:5854396-5854415 Msa0832760:intron 25.0%
!!! TTGATTAGTTTAATGATGAG+AGG + chr5_4:5854452-5854471 Msa0832760:intron 25.0%
!!! TGATTAGTTTAATGATGAGA+GGG + chr5_4:5854453-5854472 Msa0832760:intron 25.0%
! ATCTAGAAAACTATGTTTGT+GGG - chr5_4:5854678-5854697 None:intergenic 25.0%
! AATCTAGAAAACTATGTTTG+TGG - chr5_4:5854679-5854698 None:intergenic 25.0%
!! CAAACATAGTTTTCTAGATT+TGG + chr5_4:5854679-5854698 Msa0832760:intron 25.0%
!!! CGAGGTTTTTTTTTCAATTT+AGG - chr5_4:5854875-5854894 None:intergenic 25.0%
!!! GATTTTCTGCATCTTTTTTA+TGG + chr5_4:5854951-5854970 Msa0832760:intron 25.0%
! TTATTCTTCAGAATACATCT+AGG + chr5_4:5855453-5855472 Msa0832760:CDS 25.0%
! TATTCTTCAGAATACATCTA+GGG + chr5_4:5855454-5855473 Msa0832760:CDS 25.0%
! TCTAGTATGTATAGAACATA+GGG + chr5_4:5855831-5855850 Msa0832760:three_prime_UTR 25.0%
!! ATTAGATTTTTGTTAGAGCT+AGG + chr5_4:5855901-5855920 Msa0832760:three_prime_UTR 25.0%
! TAAACTTCAGATATACTCTT+AGG - chr5_4:5856075-5856094 None:intergenic 25.0%
! GAATGGTAATAATGGAAAAG+AGG - chr5_4:5853147-5853166 None:intergenic 30.0%
ATCTCAATCTCAATCTTCTA+TGG + chr5_4:5853174-5853193 Msa0832760:exon 30.0%
TCTCAATCTCAATCTTCTAT+GGG + chr5_4:5853175-5853194 Msa0832760:exon 30.0%
TAATTCACCTAAAGTTGAAC+CGG - chr5_4:5853242-5853261 None:intergenic 30.0%
! TCTCTTTTACCCAAATTGAT+TGG - chr5_4:5853433-5853452 None:intergenic 30.0%
CGACTTAATACTATTGATGA+TGG + chr5_4:5853472-5853491 Msa0832760:CDS 30.0%
TCCATTTCGCGAAAAATTTA+GGG - chr5_4:5853553-5853572 None:intergenic 30.0%
ATCCATTTCGCGAAAAATTT+AGG - chr5_4:5853554-5853573 None:intergenic 30.0%
!!! ATTTTGGTTTAGCGTATGTT+TGG + chr5_4:5853645-5853664 Msa0832760:intron 30.0%
! TGTATGTATGATTCCACTTT+TGG - chr5_4:5853840-5853859 None:intergenic 30.0%
TGGAATCATACATACACTTA+AGG + chr5_4:5853844-5853863 Msa0832760:intron 30.0%
ATATCATCACTGAAGAATCT+AGG + chr5_4:5854277-5854296 Msa0832760:CDS 30.0%
!! GATTAGTTTAATGATGAGAG+GGG + chr5_4:5854454-5854473 Msa0832760:intron 30.0%
TCTAGAAAACTATGTTTGTG+GGG - chr5_4:5854677-5854696 None:intergenic 30.0%
GACTAACACATGTTGATATT+TGG - chr5_4:5854759-5854778 None:intergenic 30.0%
GTGAAAATTGGTAAAGTGAT+AGG + chr5_4:5855031-5855050 Msa0832760:intron 30.0%
ATCATCAACTGCGAAAATTA+AGG - chr5_4:5855251-5855270 None:intergenic 30.0%
!!! AATCTATTTTAGTGGTGCTT+TGG + chr5_4:5855318-5855337 Msa0832760:intron 30.0%
! AGTCATAGAGAATTTGACTT+TGG - chr5_4:5855673-5855692 None:intergenic 30.0%
! CATTTTCTTTCTACCTGAAA+TGG - chr5_4:5855798-5855817 None:intergenic 30.0%
GTCTAGTATGTATAGAACAT+AGG + chr5_4:5855830-5855849 Msa0832760:three_prime_UTR 30.0%
ACATCAGACACAAATGTATA+TGG - chr5_4:5856146-5856165 None:intergenic 30.0%
TTTGAGTACAGTAGAATGAA+TGG + chr5_4:5856165-5856184 Msa0832760:three_prime_UTR 30.0%
TTGAGTACAGTAGAATGAAT+GGG + chr5_4:5856166-5856185 Msa0832760:three_prime_UTR 30.0%
GGTGCAGAAAGATTGAAAAA+GGG - chr5_4:5853125-5853144 None:intergenic 35.0%
!! TTCTTCTGCTGATTTCGTTT+GGG + chr5_4:5853351-5853370 Msa0832760:CDS 35.0%
! AGGAGAAGATGGTAATTTGT+CGG - chr5_4:5853388-5853407 None:intergenic 35.0%
!! AATCATGAAAGATTTTGGCG+AGG - chr5_4:5853408-5853427 None:intergenic 35.0%
! ACCCTAAATTTTTCGCGAAA+TGG + chr5_4:5853549-5853568 Msa0832760:intron 35.0%
! GGAGCCAAAATTGATTCTAA+AGG - chr5_4:5853816-5853835 None:intergenic 35.0%
!! ATGTATGATTCCACTTTTGG+AGG - chr5_4:5853837-5853856 None:intergenic 35.0%
ATCATACATACACTTAAGGC+TGG + chr5_4:5853848-5853867 Msa0832760:intron 35.0%
CTTATCTCAGCTTATAACAC+AGG - chr5_4:5854090-5854109 None:intergenic 35.0%
! TTTGTTGTGTGCAGAAAGTT+TGG + chr5_4:5854163-5854182 Msa0832760:intron 35.0%
TTGGCTATATCAGCTAACAA+GGG + chr5_4:5854213-5854232 Msa0832760:CDS 35.0%
AACAGAATTGATCACAATGC+AGG - chr5_4:5854344-5854363 None:intergenic 35.0%
!! AAGGTAATGGAGAGAACTAA+GGG + chr5_4:5854477-5854496 Msa0832760:intron 35.0%
GACTGGAATACATTCAAGTA+AGG + chr5_4:5854619-5854638 Msa0832760:intron 35.0%
AACATGTGTTAGTCCAAGTA+TGG + chr5_4:5854765-5854784 Msa0832760:intron 35.0%
ACATGTGTTAGTCCAAGTAT+GGG + chr5_4:5854766-5854785 Msa0832760:intron 35.0%
ACCTGTAACCACATGTTTAA+GGG - chr5_4:5854800-5854819 None:intergenic 35.0%
AACCTGTAACCACATGTTTA+AGG - chr5_4:5854801-5854820 None:intergenic 35.0%
!! CACTTGATGATGGTGAAAAT+TGG + chr5_4:5855019-5855038 Msa0832760:intron 35.0%
GATAGGACATCGTTTGAAAA+TGG + chr5_4:5855048-5855067 Msa0832760:intron 35.0%
! TTGTTTGAGTTCAACCACAT+TGG + chr5_4:5855208-5855227 Msa0832760:intron 35.0%
AGCAAGCATATTTACCAATG+TGG - chr5_4:5855225-5855244 None:intergenic 35.0%
AGTTCCTTTCAGAAAACACT+GGG - chr5_4:5855592-5855611 None:intergenic 35.0%
! AATTGGACTTTTTACCATGC+AGG + chr5_4:5855615-5855634 Msa0832760:CDS 35.0%
! TGCTCTTTTTGAATTCGCTT+GGG - chr5_4:5855752-5855771 None:intergenic 35.0%
! TTGCTCTTTTTGAATTCGCT+TGG - chr5_4:5855753-5855772 None:intergenic 35.0%
TCTTTCTACCTGAAATGGTT+AGG - chr5_4:5855793-5855812 None:intergenic 35.0%
AGTATGTATAGAACATAGGG+CGG + chr5_4:5855834-5855853 Msa0832760:three_prime_UTR 35.0%
! AGATTTTTGTTAGAGCTAGG+AGG + chr5_4:5855904-5855923 Msa0832760:three_prime_UTR 35.0%
! GATTTTTGTTAGAGCTAGGA+GGG + chr5_4:5855905-5855924 Msa0832760:three_prime_UTR 35.0%
TTGAGAAAGTTCTATGCTTG+CGG + chr5_4:5856011-5856030 Msa0832760:three_prime_UTR 35.0%
TTGCGGAAGATTTGAATGTA+TGG + chr5_4:5856028-5856047 Msa0832760:three_prime_UTR 35.0%
TGCGGAAGATTTGAATGTAT+GGG + chr5_4:5856029-5856048 Msa0832760:three_prime_UTR 35.0%
! ATGAATGGGAATAAGAGTGT+TGG + chr5_4:5856180-5856199 Msa0832760:three_prime_UTR 35.0%
GGGTGCAGAAAGATTGAAAA+AGG - chr5_4:5853126-5853145 None:intergenic 40.0%
TCATTTCGATCCAACCAAAC+CGG + chr5_4:5853211-5853230 Msa0832760:CDS 40.0%
TAAAGTTGAACCGGTTGAAC+CGG - chr5_4:5853233-5853252 None:intergenic 40.0%
!! CTTCTTCTGCTGATTTCGTT+TGG + chr5_4:5853350-5853369 Msa0832760:CDS 40.0%
!! TACTATTGATGATGGCACCA+AGG + chr5_4:5853480-5853499 Msa0832760:CDS 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + chr5_4:5853824-5853843 Msa0832760:intron 40.0%
! ACATACACTTAAGGCTGGTT+CGG + chr5_4:5853853-5853872 Msa0832760:intron 40.0%
GATAAGCTGTTTGTCCAAAC+AGG + chr5_4:5854104-5854123 Msa0832760:intron 40.0%
GTTTGTCCAAACAGGCTTAT+TGG + chr5_4:5854112-5854131 Msa0832760:intron 40.0%
! AAACAGGCTTATTGGCTGAT+GGG + chr5_4:5854120-5854139 Msa0832760:intron 40.0%
AGTTTGGATTGATAGAGCCA+CGG + chr5_4:5854179-5854198 Msa0832760:CDS 40.0%
CTTGGCTATATCAGCTAACA+AGG + chr5_4:5854212-5854231 Msa0832760:CDS 40.0%
AACAAGGGACTATCAGTACT+TGG + chr5_4:5854228-5854247 Msa0832760:CDS 40.0%
AGTTTAATGATGAGAGGGGA+AGG + chr5_4:5854458-5854477 Msa0832760:intron 40.0%
!! GAAGGTAATGGAGAGAACTA+AGG + chr5_4:5854476-5854495 Msa0832760:intron 40.0%
TAATGGAGAGAACTAAGGGA+GGG + chr5_4:5854481-5854500 Msa0832760:intron 40.0%
CATGTGTTAGTCCAAGTATG+GGG + chr5_4:5854767-5854786 Msa0832760:intron 40.0%
ATGTGTTAGTCCAAGTATGG+GGG + chr5_4:5854768-5854787 Msa0832760:intron 40.0%
AACCACATGTTTAAGGGACA+CGG - chr5_4:5854794-5854813 None:intergenic 40.0%
TCCCTTAAACATGTGGTTAC+AGG + chr5_4:5854796-5854815 Msa0832760:intron 40.0%
TACAGGTTCAATTCATACCC+AGG + chr5_4:5854813-5854832 Msa0832760:intron 40.0%
ACAGGTTCAATTCATACCCA+GGG + chr5_4:5854814-5854833 Msa0832760:intron 40.0%
! TTCTTCCATCGACTAGTCAA+GGG - chr5_4:5854848-5854867 None:intergenic 40.0%
! ATTCTTCCATCGACTAGTCA+AGG - chr5_4:5854849-5854868 None:intergenic 40.0%
CTTTGTCGGTACTATTGTGA+AGG + chr5_4:5855171-5855190 Msa0832760:intron 40.0%
GAAGAATAAGCTGTATGTCC+CGG - chr5_4:5855442-5855461 None:intergenic 40.0%
TGCAACACTGAACATGTTCA+TGG + chr5_4:5855507-5855526 Msa0832760:CDS 40.0%
GCAACACTGAACATGTTCAT+GGG + chr5_4:5855508-5855527 Msa0832760:CDS 40.0%
TTCCAACTTTGGACTTCAGA+TGG + chr5_4:5855552-5855571 Msa0832760:CDS 40.0%
TCCAACTTTGGACTTCAGAT+GGG + chr5_4:5855553-5855572 Msa0832760:CDS 40.0%
CAGTTCCTTTCAGAAAACAC+TGG - chr5_4:5855593-5855612 None:intergenic 40.0%
!! CAGTGTTTTCTGAAAGGAAC+TGG + chr5_4:5855591-5855610 Msa0832760:CDS 40.0%
! TTCTGAAAGGAACTGGCAAT+TGG + chr5_4:5855598-5855617 Msa0832760:CDS 40.0%
TGATGAATTCCCATCCTCAA+CGG - chr5_4:5855649-5855668 None:intergenic 40.0%
ATTGATTGCACGCACACTAA+AGG + chr5_4:5855696-5855715 Msa0832760:CDS 40.0%
AAACTGCTCCTAACCATTTC+AGG + chr5_4:5855782-5855801 Msa0832760:exon 40.0%
ATAGGATAGTACCGCTGAGT+TGG + chr5_4:5852981-5853000 Msa0832760:five_prime_UTR 45.0%
! GGTTCAACCGGTTCAACTTT+AGG + chr5_4:5853232-5853251 Msa0832760:CDS 45.0%
ATTGAAGCGACGCAACTTTC+AGG - chr5_4:5853265-5853284 None:intergenic 45.0%
AGTTGCGTCGCTTCAATCAT+CGG + chr5_4:5853268-5853287 Msa0832760:CDS 45.0%
! CGCTTCAATCATCGGTTACA+TGG + chr5_4:5853276-5853295 Msa0832760:CDS 45.0%
! GTTGCAAGTTGACAGATCTG+AGG - chr5_4:5853307-5853326 None:intergenic 45.0%
!! AGATTTTGGCGAGGAGAAGA+TGG - chr5_4:5853399-5853418 None:intergenic 45.0%
CATCAGCCAATAAGCCTGTT+TGG - chr5_4:5854121-5854140 None:intergenic 45.0%
! CAAACAGGCTTATTGGCTGA+TGG + chr5_4:5854119-5854138 Msa0832760:intron 45.0%
!! GGCTGATGGGTTTGGATATA+TGG + chr5_4:5854133-5854152 Msa0832760:intron 45.0%
GTTTGGATTGATAGAGCCAC+GGG + chr5_4:5854180-5854199 Msa0832760:CDS 45.0%
ATGATGAGAGGGGAAGGTAA+TGG + chr5_4:5854464-5854483 Msa0832760:intron 45.0%
! GTAATGGAGAGAACTAAGGG+AGG + chr5_4:5854480-5854499 Msa0832760:intron 45.0%
AATGGAGAGAACTAAGGGAG+GGG + chr5_4:5854482-5854501 Msa0832760:intron 45.0%
GTCAAGGGATCAATTACCCT+GGG - chr5_4:5854833-5854852 None:intergenic 45.0%
AGTCAAGGGATCAATTACCC+TGG - chr5_4:5854834-5854853 None:intergenic 45.0%
! TTGATCCCTTGACTAGTCGA+TGG + chr5_4:5854840-5854859 Msa0832760:intron 45.0%
ATCATCAAGTGCGCGAGAAT+AGG - chr5_4:5855010-5855029 None:intergenic 45.0%
TATTCTCGCGCACTTGATGA+TGG + chr5_4:5855009-5855028 Msa0832760:intron 45.0%
ATTACGCTTCTCTGCTACTG+AGG + chr5_4:5855113-5855132 Msa0832760:intron 45.0%
TTGCATACCTTCAGCATACC+TGG + chr5_4:5855376-5855395 Msa0832760:CDS 45.0%
ACACTGAACATGTTCATGGG+TGG + chr5_4:5855511-5855530 Msa0832760:CDS 45.0%
CACTGAACATGTTCATGGGT+GGG + chr5_4:5855512-5855531 Msa0832760:CDS 45.0%
TCCTTTCAGAAAACACTGGG+AGG - chr5_4:5855589-5855608 None:intergenic 45.0%
GTGATTTCACCGTTGAGGAT+GGG + chr5_4:5855637-5855656 Msa0832760:CDS 45.0%
CACTAAAGGTGGTCTGTGTT+TGG + chr5_4:5855710-5855729 Msa0832760:CDS 45.0%
! ACCGCTGAGTTGGCATTTGT+CGG + chr5_4:5852991-5853010 Msa0832760:five_prime_UTR 50.0%
TCCGACAAATGCCAACTCAG+CGG - chr5_4:5852995-5853014 None:intergenic 50.0%
!! GTGGACTCGGTTTGGTATGA+TGG - chr5_4:5853042-5853061 None:intergenic 50.0%
ATACCAAACCGAGTCCACCA+AGG + chr5_4:5853044-5853063 Msa0832760:five_prime_UTR 50.0%
ATGTACAGCAACGCGTACAC+CGG - chr5_4:5853074-5853093 None:intergenic 50.0%
! TCCAACCAAACCGGTTCAAC+CGG + chr5_4:5853220-5853239 Msa0832760:CDS 50.0%
TTGAACCGGTTGAACCGGTT+TGG - chr5_4:5853228-5853247 None:intergenic 50.0%
! GCAAGTTGACAGATCTGAGG+AGG - chr5_4:5853304-5853323 None:intergenic 50.0%
!! CACTTAAGGCTGGTTCGGAT+TGG + chr5_4:5853858-5853877 Msa0832760:intron 50.0%
!! GGCTTATTGGCTGATGGGTT+TGG + chr5_4:5854125-5854144 Msa0832760:intron 50.0%
ATGGAGAGAACTAAGGGAGG+GGG + chr5_4:5854483-5854502 Msa0832760:intron 50.0%
CATACCTTCAGCATACCTGG+TGG + chr5_4:5855379-5855398 Msa0832760:CDS 50.0%
CAAACCACCAGGTATGCTGA+AGG - chr5_4:5855386-5855405 None:intergenic 50.0%
GCATACCTGGTGGTTTGAAG+TGG + chr5_4:5855389-5855408 Msa0832760:CDS 50.0%
! ACCTGGTGGTTTGAAGTGGA+CGG + chr5_4:5855393-5855412 Msa0832760:CDS 50.0%
! GTTGACTTCCCATTTCCAGC+CGG + chr5_4:5855420-5855439 Msa0832760:CDS 50.0%
TTGACTTCCCATTTCCAGCC+GGG + chr5_4:5855421-5855440 Msa0832760:CDS 50.0%
TGTATGTCCCGGCTGGAAAT+GGG - chr5_4:5855431-5855450 None:intergenic 50.0%
AATAAGCTGTATGTCCCGGC+TGG - chr5_4:5855438-5855457 None:intergenic 50.0%
! ATCTAGGGCAAGCCTACAAG+AGG + chr5_4:5855469-5855488 Msa0832760:CDS 50.0%
ACGACGTCCAAACCTCTTGT+AGG - chr5_4:5855484-5855503 None:intergenic 50.0%
AGCCTGTGCGATTCCAACTT+TGG + chr5_4:5855541-5855560 Msa0832760:CDS 50.0%
GTCCAAAGTTGGAATCGCAC+AGG - chr5_4:5855546-5855565 None:intergenic 50.0%
! GCCCATCTGAAGTCCAAAGT+TGG - chr5_4:5855557-5855576 None:intergenic 50.0%
! GCCTCCCAGTGTTTTCTGAA+AGG + chr5_4:5855585-5855604 Msa0832760:CDS 50.0%
CAACGGTGAAATCACCTGCA+TGG - chr5_4:5855632-5855651 None:intergenic 50.0%
TGCAGGTGATTTCACCGTTG+AGG + chr5_4:5855632-5855651 Msa0832760:CDS 50.0%
GGTGATTTCACCGTTGAGGA+TGG + chr5_4:5855636-5855655 Msa0832760:CDS 50.0%
GATTGCACGCACACTAAAGG+TGG + chr5_4:5855699-5855718 Msa0832760:CDS 50.0%
!! TGTCCTTGGTGGACTCGGTT+TGG - chr5_4:5853050-5853069 None:intergenic 55.0%
!! ACCGGTTGAACCGGTTTGGT+TGG - chr5_4:5853224-5853243 None:intergenic 55.0%
AGAGAACTAAGGGAGGGGGA+AGG + chr5_4:5854487-5854506 Msa0832760:intron 55.0%
GGCCGTGTCCCTTAAACATG+TGG + chr5_4:5854789-5854808 Msa0832760:intron 55.0%
GCTTCTCTGCTACTGAGGCA+CGG + chr5_4:5855118-5855137 Msa0832760:intron 55.0%
! CCTGGTGGTTTGAAGTGGAC+GGG + chr5_4:5855394-5855413 Msa0832760:CDS 55.0%
! CTGGTGGTTTGAAGTGGACG+GGG + chr5_4:5855395-5855414 Msa0832760:CDS 55.0%
CTGTATGTCCCGGCTGGAAA+TGG - chr5_4:5855432-5855451 None:intergenic 55.0%
GGGCAAGCCTACAAGAGGTT+TGG + chr5_4:5855474-5855493 Msa0832760:CDS 55.0%
!! GAACATAGGGCGGTGTCATG+AGG + chr5_4:5855844-5855863 Msa0832760:three_prime_UTR 55.0%
!! CCGGTTGTCCTTGGTGGACT+CGG - chr5_4:5853055-5853074 None:intergenic 60.0%
CCGAGTCCACCAAGGACAAC+CGG + chr5_4:5853052-5853071 Msa0832760:five_prime_UTR 60.0%
! CGTACACCGGTTGTCCTTGG+TGG - chr5_4:5853061-5853080 None:intergenic 60.0%
! ACGCGTACACCGGTTGTCCT+TGG - chr5_4:5853064-5853083 None:intergenic 60.0%
AGCCACGGGCAAGCTTTGCT+TGG + chr5_4:5854194-5854213 Msa0832760:CDS 60.0%
AGCCAAGCAAAGCTTGCCCG+TGG - chr5_4:5854199-5854218 None:intergenic 60.0%
AAGGGAGGGGGAAGGAATCG+AGG + chr5_4:5854495-5854514 Msa0832760:intron 60.0%
AGGGAGGGGGAAGGAATCGA+GGG + chr5_4:5854496-5854515 Msa0832760:intron 60.0%
AGGGGGAAGGAATCGAGGGA+TGG + chr5_4:5854500-5854519 Msa0832760:intron 60.0%
CCCGTCCACTTCAAACCACC+AGG - chr5_4:5855397-5855416 None:intergenic 60.0%
AGGGACACGGCCCCCATACT+TGG - chr5_4:5854781-5854800 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 5852973 5856214 5852973 ID=Msa0832760;Name=Msa0832760
chr5_4 mRNA 5852973 5856214 5852973 ID=Msa0832760-mRNA-1;Parent=Msa0832760;Name=Msa0832760-mRNA-1;_AED=0.03;_eAED=0.03;_QI=220|1|1|1|1|1|3|420|286
chr5_4 exon 5852973 5853501 5852973 ID=Msa0832760-mRNA-1:exon:2487;Parent=Msa0832760-mRNA-1
chr5_4 exon 5854177 5854298 5854177 ID=Msa0832760-mRNA-1:exon:2488;Parent=Msa0832760-mRNA-1
chr5_4 exon 5855365 5856214 5855365 ID=Msa0832760-mRNA-1:exon:2489;Parent=Msa0832760-mRNA-1
chr5_4 five_prime_UTR 5852973 5853192 5852973 ID=Msa0832760-mRNA-1:five_prime_utr;Parent=Msa0832760-mRNA-1
chr5_4 CDS 5853193 5853501 5853193 ID=Msa0832760-mRNA-1:cds;Parent=Msa0832760-mRNA-1
chr5_4 CDS 5854177 5854298 5854177 ID=Msa0832760-mRNA-1:cds;Parent=Msa0832760-mRNA-1
chr5_4 CDS 5855365 5855794 5855365 ID=Msa0832760-mRNA-1:cds;Parent=Msa0832760-mRNA-1
chr5_4 three_prime_UTR 5855795 5856214 5855795 ID=Msa0832760-mRNA-1:three_prime_utr;Parent=Msa0832760-mRNA-1
Gene Sequence

>Msa0832760

ATGGGTATTCGCTTTTCTTCATTTCGATCCAACCAAACCGGTTCAACCGGTTCAACTTTAGGTGAATTACCTGAAAGTTGCGTCGCTTCAATCATCGGTTACATGGATCCTCCTCAGATCTGTCAACTTGCAACGTTGAATCGTGCTTTTCGTGCTGCTTCTTCTGCTGATTTCGTTTGGGAATCTAAATTGCCGACAAATTACCATCTTCTCCTCGCCAAAATCTTTCATGATTTACCAATCAATTTGGGTAAAAGAGACATTTATGCTTCTCTTTGTCGACTTAATACTATTGATGATGGCACCAAGAAAGTTTGGATTGATAGAGCCACGGGCAAGCTTTGCTTGGCTATATCAGCTAACAAGGGACTATCAGTACTTGGAATTGATGATAGAAGATATTGGAATTATATCATCACTGAAGAATCTAGATTCAATACTGTTGCATACCTTCAGCATACCTGGTGGTTTGAAGTGGACGGGGAAGTTGACTTCCCATTTCCAGCCGGGACATACAGCTTATTCTTCAGAATACATCTAGGGCAAGCCTACAAGAGGTTTGGACGTCGTGTCTGCAACACTGAACATGTTCATGGGTGGGACAAAAAGCCTGTGCGATTCCAACTTTGGACTTCAGATGGGCAATACGTTGCCTCCCAGTGTTTTCTGAAAGGAACTGGCAATTGGACTTTTTACCATGCAGGTGATTTCACCGTTGAGGATGGGAATTCATCAACCAAAGTCAAATTCTCTATGACTCAAATTGATTGCACGCACACTAAAGGTGGTCTGTGTTTGGATTCTGCTTTTATTTACCCAAGCGAATTCAAAAAGAGCAAGTCATTTTTAAACTGCTCCTAA
Protein sequence

>Msa0832760

MGIRFSSFRSNQTGSTGSTLGELPESCVASIIGYMDPPQICQLATLNRAFRAASSADFVWESKLPTNYHLLLAKIFHDLPINLGKRDIYASLCRLNTIDDGTKKVWIDRATGKLCLAISANKGLSVLGIDDRRYWNYIITEESRFNTVAYLQHTWWFEVDGEVDFPFPAGTYSLFFRIHLGQAYKRFGRRVCNTEHVHGWDKKPVRFQLWTSDGQYVASQCFLKGTGNWTFYHAGDFTVEDGNSSTKVKFSMTQIDCTHTKGGLCLDSAFIYPSEFKKSKSFLNCS*