AlfalfaGEDB | Msa0303150

M. sativa cultivar zhongmu-4 / Msa0303150

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02042.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0303150	XP_003597538.2	94.840	969	44	2	1	968	1	964	0.0	1862

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0303150	sp\|O22864\|NLP8_ARATH	48.293	996	432	22	1	965	1	944	0.0	799

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0303150	TF	RWP-RK

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002230	Msa0303150	0.806124	9.577747e-50	-8.615850e-47
Msa0006110	Msa0303150	0.825389	4.813980e-54	-8.615850e-47
Msa0007440	Msa0303150	0.833904	4.078266e-56	-8.615850e-47
Msa0011170	Msa0303150	0.800953	1.131431e-48	-8.615850e-47
Msa0014710	Msa0303150	0.801439	9.000936e-49	-8.615850e-47
Msa0015090	Msa0303150	0.845608	3.651316e-59	-8.615850e-47
Msa0017320	Msa0303150	0.813797	2.134177e-51	-8.615850e-47
Msa0018070	Msa0303150	0.808315	3.289409e-50	-8.615850e-47
Msa0021210	Msa0303150	0.855033	8.274931e-62	-8.615850e-47
Msa0023330	Msa0303150	0.813126	2.996305e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0303150	MtrunA17_Chr2g0329671	94.840	969	44	2	1	968	1	964	0.0	1862
Msa0303150	MtrunA17_Chr4g0033791	59.792	960	322	21	1	950	1	906	0.0	1060
Msa0303150	MtrunA17_Chr6g0472281	35.201	821	419	17	177	961	112	855	7.01e-144	450
Msa0303150	MtrunA17_Chr1g0203081	39.799	598	306	18	177	745	124	696	9.50e-120	389
Msa0303150	MtrunA17_Chr1g0203081	45.745	94	50	1	869	961	884	977	2.71e-17	87.4
Msa0303150	MtrunA17_Chr3g0143921	39.123	570	286	15	176	701	138	690	3.02e-118	384
Msa0303150	MtrunA17_Chr5g0448621	31.044	364	205	12	173	526	122	449	4.12e-44	173
Msa0303150	MtrunA17_Chr5g0448621	67.778	90	25	1	602	691	607	692	6.18e-31	131

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0303150	AT2G43500.3	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.9	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.6	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.1	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.10	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.11	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.7	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.8	48.932	983	432	20	1	965	1	931	810
Msa0303150	AT2G43500.5	48.293	996	432	21	1	965	1	944	800
Msa0303150	AT2G43500.4	48.293	996	432	21	1	965	1	944	800

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Find 278 sgRNAs with CRISPR-Local

Find 553 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGATATGCAGGCATCA+CGG	0.641252	2_3:+60323315	Msa0303150:CDS
AAACGAGCCTTCTTGTAATC+AGG	0.361894	2_3:+60321492	Msa0303150:CDS
AAACGTAAACATAGTACTAT+TGG	0.410618	2_3:+60323765	Msa0303150:CDS
AAAGTTGTCACTACTTGACT+TGG	0.517094	2_3:-60324820	None:intergenic
AAATCAACCATAGTTGATAG+CGG	0.669638	2_3:+60323972	Msa0303150:CDS
AACACACGGCCAGGAAGACC+CGG	0.580655	2_3:-60318882	None:intergenic
AACATAGTACTATTGGCCAG+AGG	0.720267	2_3:+60323772	Msa0303150:CDS
AACTATGGTTGATTTGCCTT+TGG	0.369589	2_3:-60323964	None:intergenic
AAGAACCAGAAGCCGTCCAA+AGG	0.607244	2_3:+60323948	Msa0303150:CDS
AAGAGTTTGAGGACAGTTTC+AGG	0.268953	2_3:+60321304	Msa0303150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGCAGTTAAAGAAATGAAG+TGG	-	chr2_3:60316895-60316914	None:intergenic	30.0%
AATCTGAACATACTGAAAGT+GGG	+	chr2_3:60317282-60317301	Msa0303150:five_prime_UTR	30.0%
ACTAGAGAGAATCAAAATTC+AGG	-	chr2_3:60317542-60317561	None:intergenic	30.0%
AAGATTTAATTGAAGAGTGG+TGG	+	chr2_3:60317572-60317591	Msa0303150:intron	30.0%
TGTGCACAAACAATTTAACA+CGG	+	chr2_3:60317731-60317750	Msa0303150:intron	30.0%
ATGAACTTTGATACTTATGC+TGG	+	chr2_3:60318285-60318304	Msa0303150:CDS	30.0%
CGGTGGAAATTACAATAATT+TGG	+	chr2_3:60318446-60318465	Msa0303150:CDS	30.0%
ATGCAAATGACTTAAGTTCT+AGG	+	chr2_3:60318547-60318566	Msa0303150:CDS	30.0%
TATTTGTATCTATATTGCCC+TGG	+	chr2_3:60319296-60319315	Msa0303150:intron	30.0%
CTTAAATGCTTATCAAACCA+GGG	-	chr2_3:60319316-60319335	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	60316803	60324829	60316803	ID=Msa0303150;Name=Msa0303150
chr2_3	mRNA	60316803	60324829	60316803	ID=Msa0303150-mRNA-1;Parent=Msa0303150;Name=Msa0303150-mRNA-1;_AED=0.08;_eAED=0.08;_QI=379\|1\|1\|1\|0.57\|0.37\|8\|433\|968
chr2_3	exon	60316803	60316993	60316803	ID=Msa0303150-mRNA-1:exon:19660;Parent=Msa0303150-mRNA-1
chr2_3	exon	60317281	60317384	60317281	ID=Msa0303150-mRNA-1:exon:19661;Parent=Msa0303150-mRNA-1
chr2_3	exon	60318060	60319127	60318060	ID=Msa0303150-mRNA-1:exon:19662;Parent=Msa0303150-mRNA-1
chr2_3	exon	60320649	60320693	60320649	ID=Msa0303150-mRNA-1:exon:19663;Parent=Msa0303150-mRNA-1
chr2_3	exon	60320767	60321513	60320767	ID=Msa0303150-mRNA-1:exon:19664;Parent=Msa0303150-mRNA-1
chr2_3	exon	60321952	60322075	60321952	ID=Msa0303150-mRNA-1:exon:19665;Parent=Msa0303150-mRNA-1
chr2_3	exon	60323298	60324664	60323298	ID=Msa0303150-mRNA-1:exon:19666;Parent=Msa0303150-mRNA-1
chr2_3	exon	60324757	60324829	60324757	ID=Msa0303150-mRNA-1:exon:19667;Parent=Msa0303150-mRNA-1
chr2_3	five_prime_UTR	60316803	60316993	60316803	ID=Msa0303150-mRNA-1:five_prime_utr;Parent=Msa0303150-mRNA-1
chr2_3	five_prime_UTR	60317281	60317384	60317281	ID=Msa0303150-mRNA-1:five_prime_utr;Parent=Msa0303150-mRNA-1
chr2_3	five_prime_UTR	60318060	60318143	60318060	ID=Msa0303150-mRNA-1:five_prime_utr;Parent=Msa0303150-mRNA-1
chr2_3	CDS	60318144	60319127	60318144	ID=Msa0303150-mRNA-1:cds;Parent=Msa0303150-mRNA-1
chr2_3	CDS	60320649	60320693	60320649	ID=Msa0303150-mRNA-1:cds;Parent=Msa0303150-mRNA-1
chr2_3	CDS	60320767	60321513	60320767	ID=Msa0303150-mRNA-1:cds;Parent=Msa0303150-mRNA-1
chr2_3	CDS	60321952	60322075	60321952	ID=Msa0303150-mRNA-1:cds;Parent=Msa0303150-mRNA-1
chr2_3	CDS	60323298	60324304	60323298	ID=Msa0303150-mRNA-1:cds;Parent=Msa0303150-mRNA-1
chr2_3	three_prime_UTR	60324305	60324664	60324305	ID=Msa0303150-mRNA-1:three_prime_utr;Parent=Msa0303150-mRNA-1
chr2_3	three_prime_UTR	60324757	60324829	60324757	ID=Msa0303150-mRNA-1:three_prime_utr;Parent=Msa0303150-mRNA-1

Gene Sequence

>Msa0303150

ATGGAATACCCTTTTTATTCTAAGGGAGGAGGAATTGGGTATTGGCAGTCTCCTGGAACTCAGTTCGAAGGGCCAACATCATTAGATGGTGGAATCAACAATTTGGTGTCAGAAGATATGGCTAATAGCTTCTCCGAGCTCATGAACTTTGATACTTATGCTGGTTTGTGTTCTGGCCCATCCATGACCGATCAGATTATGACCAATGAGCTTCCATCTCTTGCCTCGGTATTATATCAATCGCCCGATGGATTCAATCCAGTCGAACAGAACAGTGGACAATTCTATATGACAGGAGGAGTCGGTGGAAATTACAATAATTTGGAAAGCTCCCCTATTTTTGGGGAGAAAATTGTGTGTCAGCAAATGGATACCCTACTTGGTTTCTTAGACAACAGCAATGATGCAAATGACTTAAGTTCTAGGCAAAAATTTGACGACCCTTCGCAACATGTCAATGCCTTTGATATGGGTAATTATGTAATCGCCAGGCCACAAGCTTTAATCTCCAGGCCACCTGCTTTGTCACTTGATGAGAGAATGCTGAAAGCCTTGTCCTTCTTTAAAGAATCAGCTGGTGGGGGAATATTGGCACAAGTTTGGGTACCCATAAAGCATGATGGTCAAGTACTCTTAAGCACCAGCGAGCAACCTTATTTACTAGATCAAATGCTTGCAGGGTATCGTGAAGTTTCAAGGACATTTAGATTTTCTACAGAAGGAAAACCAGGTTGTATACCGGGTCTTCCTGGCCGTGTGTTTATCTCCAAAGTTCCTGAGTGGACTTCCAATGTTGGTTATTACAAACCAAAGGAGTACTTGAGGGCTGAGCATGCAAGAAATCACGAGGTTCGTGGATCAATTGCTTTTCCCATATTTGATCTGCACTCTGGACTGCCATGTTCTGCTGTCTTGGAACTTGTCACTACAAAGGAAAAGCTTGATTTTGACAGAGAGTTGGAAATCATTTGTCGGTCACTTCAGCTTGTAAATTTAAGGACTACTGTGTCTTCTCGGCTGCTTCCTGAGTGTCTTTCGACCAATAAAAGAGCTGCTTTAACCGAGATAGTGGATGTGTTACGATCTGTGTGTCATGCACATAGATTGCCATTGGCACTAACATGGATTCCCTGTTTTTACACCGACGGCACAAGAGATGTAACTACTAGAATACAAATTAAAGAGGGTAATTCAAGTTCTAGAGAAAAAAACATACTGTGTATTGAAGAATCAGCTTGCTATATAACTGATAAAGTGATGGAAGGATTTGTGCGTGCATGCATTGAACATCCTCTTGAGGAAGGGAAAGGTGTAGCTGGGAAAGCACTTCAATCAAACCATCCTTTCTTCTATTCTGACGTGAAAACATATGATATTAGTGAGTATCCGCTTGTTCATCATGCACGCAAGTTCAATTTGGGTGCTGCAGTTGCAATCAGGCTAAGGAGTATTTATACTAATAATGATGATTACATATTGGAATTCTTTCTTCCTATCAATATGAAAGGGAGTCAAGAACAGCAACTCTTATTAGACAACCTCTCAGGTACCATGCAGAAAATTTGTAAGAGTTTGAGGACAGTTTCAGGTGCTGAAATATCAGGAACAGAAAGCTCAGTGGCGGGGTTTGGAAAGAAAAATGTCCCAAGTTTCCCCCCCACATCTACGTGGAACTCTCAGATATCATTAATAAATGAAAAGGATGGTTCTGTTCAGAAGCTATCATTGGATGCTTCTAACCTGAGAAACAATGGAAACGAGCCTTCTTGTAATCAGGAAAATAATGGATCGAGAAGGCGAGTTGAGAAAAAAAGAAACACATCAGAGAAGAATGTTAGCTTGAGTGTTCTCCAGCAATACTTTTCTGGTAGTCTGAAGGATGCTGCAAAAAGCATTGGTGTTTGCCCGACAACTCTGAAAAGGATATGCAGGCATCACGGAATTTCAAGATGGCCATCCCGCAAGATTAATAAAGTGAATCGATCTTTGAAGAAAATTCAGACTGTGCTGGACTCTGTTCAGGGAGTTGAAGGCGGGTTGAAGTTTGATCCTTCCTCGGGAGCATTTGTGGCAGGAGGGACAACCATACAAGAAATCAATGAAAATAGAAGTCTCCTTTTCCCCGAGAGAAGTACTACACAAGACTCTGAGCCTATTTCACAAGATGCTGTCTCGGTGCCTAGTGAAGGTGAGAATTCGGAAAATAAGTTGGAGGGAAAATTGAAGGAAACCAATACTTGTTCGATCAATTGCAGTGAAGATTCAAAATCATTTGGAATGGATGATTGTCCTGAACAAGCTTGTAATGGCTCCGTTCTTGCAAAATGTGATGAATGGATTCTAAACAATGGCGGTTTGAGAGAAGAAAAACGTAAACATAGTACTATTGGCCAGAGGACAAGTTCCTTTGCTGTTGATGAGATGGACACTGGTGCGGATGGGGATGATGATGTTGTTGAACACAACCATCCTACTTCTTCAAGCTTGACAGATTCGTCTAATGGCTCTGGTTCAATGATACATGGCAGTTCATCAGATTATCAGAACTTTAAGAACCAGAAGCCGTCCAAAGGCAAATCAACCATAGTTGATAGCGGGTCTAAAATCGTCGTGAAAGCTACCTATGGAGAAGACACCATTCGTTTCAAGTTTGATCCATCCACAGGTTATTATAAACTATATGAAGAAGTTGCAGCAAGATTTAAACTACAAGACGGATCATTCCAGCTCAAATATCTAGATGATGAAGAAGAATGGGTGATGTTAGTGAACGACTCGGATTTGCAAGTGTGTTTGGAAATATTGAATGACATGGGGACACATAATGCCAGGTTTCTTGTTCGTGATATTCCTTGTACGTTTGGCAGCTCTGGCAGTAGCAGTTGCTACTTGGGAGGCAGCTCATAG

Protein sequence

>Msa0303150

MEYPFYSKGGGIGYWQSPGTQFEGPTSLDGGINNLVSEDMANSFSELMNFDTYAGLCSGPSMTDQIMTNELPSLASVLYQSPDGFNPVEQNSGQFYMTGGVGGNYNNLESSPIFGEKIVCQQMDTLLGFLDNSNDANDLSSRQKFDDPSQHVNAFDMGNYVIARPQALISRPPALSLDERMLKALSFFKESAGGGILAQVWVPIKHDGQVLLSTSEQPYLLDQMLAGYREVSRTFRFSTEGKPGCIPGLPGRVFISKVPEWTSNVGYYKPKEYLRAEHARNHEVRGSIAFPIFDLHSGLPCSAVLELVTTKEKLDFDRELEIICRSLQLVNLRTTVSSRLLPECLSTNKRAALTEIVDVLRSVCHAHRLPLALTWIPCFYTDGTRDVTTRIQIKEGNSSSREKNILCIEESACYITDKVMEGFVRACIEHPLEEGKGVAGKALQSNHPFFYSDVKTYDISEYPLVHHARKFNLGAAVAIRLRSIYTNNDDYILEFFLPINMKGSQEQQLLLDNLSGTMQKICKSLRTVSGAEISGTESSVAGFGKKNVPSFPPTSTWNSQISLINEKDGSVQKLSLDASNLRNNGNEPSCNQENNGSRRRVEKKRNTSEKNVSLSVLQQYFSGSLKDAAKSIGVCPTTLKRICRHHGISRWPSRKINKVNRSLKKIQTVLDSVQGVEGGLKFDPSSGAFVAGGTTIQEINENRSLLFPERSTTQDSEPISQDAVSVPSEGENSENKLEGKLKETNTCSINCSEDSKSFGMDDCPEQACNGSVLAKCDEWILNNGGLREEKRKHSTIGQRTSSFAVDEMDTGADGDDDVVEHNHPTSSSLTDSSNGSGSMIHGSSSDYQNFKNQKPSKGKSTIVDSGSKIVVKATYGEDTIRFKFDPSTGYYKLYEEVAARFKLQDGSFQLKYLDDEEEWVMLVNDSDLQVCLEILNDMGTHNARFLVRDIPCTFGSSGSSSCYLGGSS*