AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0995520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0995520 MtrunA17_Chr7g0236991 68.488 549 173 0 1 549 4 552 0.0 811
Msa0995520 MtrunA17_Chr6g0465441 68.488 549 173 0 1 549 4 552 0.0 810
Msa0995520 MtrunA17_Chr7g0237001 68.306 549 174 0 1 549 4 552 0.0 808
Msa0995520 MtrunA17_Chr7g0237041 68.306 549 174 0 1 549 4 552 0.0 808
Msa0995520 MtrunA17_Chr7g0237081 68.306 549 174 0 1 549 4 552 0.0 808
Msa0995520 MtrunA17_Chr7g0237051 68.306 549 174 0 1 549 4 552 0.0 808
Msa0995520 MtrunA17_Chr5g0425371 70.370 540 160 0 10 549 13 552 0.0 803
Msa0995520 MtrunA17_Chr6g0465911 68.124 549 175 0 1 549 4 552 0.0 797
Msa0995520 MtrunA17_Chr6g0466051 67.942 549 176 0 1 549 4 552 0.0 795
Msa0995520 MtrunA17_Chr4g0024691 69.118 544 168 0 6 549 17 560 0.0 793
Msa0995520 MtrunA17_Chr6g0451711 67.213 549 179 1 2 549 8 556 0.0 792
Msa0995520 MtrunA17_Chr3g0105071 66.667 549 182 1 1 548 7 555 0.0 776
Msa0995520 MtrunA17_Chr6g0465851 69.052 517 160 0 33 549 1 517 0.0 756
Msa0995520 MtrunA17_Chr3g0082691 65.036 552 190 2 1 549 1 552 0.0 756
Msa0995520 MtrunA17_Chr5g0431571 65.993 544 184 1 6 548 4 547 0.0 744
Msa0995520 MtrunA17_Chr7g0236961 69.618 497 151 0 53 549 1 497 0.0 744
Msa0995520 MtrunA17_Chr5g0431651 64.428 551 194 2 1 549 1 551 0.0 743
Msa0995520 MtrunA17_Chr7g0237011 69.618 497 151 0 53 549 1 497 0.0 743
Msa0995520 MtrunA17_Chr4g0042111 58.029 548 227 2 4 549 15 561 0.0 667
Msa0995520 MtrunA17_Chr7g0236971 69.500 400 122 0 150 549 1 400 0.0 603
Msa0995520 MtrunA17_Chr6g0465981 68.919 370 115 0 1 370 4 373 0.0 545
Msa0995520 MtrunA17_Chr6g0465871 68.919 370 115 0 1 370 4 373 0.0 545
Msa0995520 MtrunA17_Chr6g0466081 68.919 370 115 0 1 370 4 373 0.0 545
Msa0995520 MtrunA17_Chr6g0466111 70.414 338 100 0 33 370 1 338 5.77e-177 502
Msa0995520 MtrunA17_Chr6g0465931 70.118 338 101 0 33 370 1 338 5.71e-176 499
Msa0995520 MtrunA17_Chr2g0319041 45.055 546 292 7 9 548 12 555 2.12e-173 501
Msa0995520 MtrunA17_Chr8g0336181 45.803 548 289 6 7 548 9 554 2.86e-170 494
Msa0995520 MtrunA17_Chr7g0236951 70.794 315 92 0 53 367 1 315 6.54e-169 481
Msa0995520 MtrunA17_Chr7g0237031 70.794 315 92 0 53 367 1 315 1.80e-168 480
Msa0995520 MtrunA17_Chr2g0319051 45.205 511 274 6 42 548 4 512 1.74e-163 475
Msa0995520 MtrunA17_Chr4g0008621 45.500 400 214 4 9 404 19 418 7.18e-124 370
Msa0995520 MtrunA17_Chr6g0466031 65.251 259 90 0 291 549 1 259 2.83e-121 357
Msa0995520 MtrunA17_Chr5g0445511 67.782 239 77 0 145 383 1 239 4.66e-119 351
Msa0995520 MtrunA17_Chr2g0319071 37.718 517 272 10 38 550 19 489 2.88e-115 350
Msa0995520 MtrunA17_Chr4g0023911 34.615 546 346 4 7 548 41 579 2.25e-114 351
Msa0995520 MtrunA17_Chr2g0323131 36.117 515 306 9 43 550 67 565 2.93e-108 335
Msa0995520 MtrunA17_Chr2g0310331 36.464 543 331 9 11 549 53 585 7.89e-108 334
Msa0995520 MtrunA17_Chr7g0237061 61.004 259 78 2 291 549 1 236 1.24e-107 322
Msa0995520 MtrunA17_Chr2g0323151 35.583 489 298 5 65 549 4 479 1.55e-104 322
Msa0995520 MtrunA17_Chr8g0357411 33.575 551 357 5 1 549 45 588 1.07e-97 308
Msa0995520 MtrunA17_Chr7g0237071 69.307 202 62 0 53 254 1 202 9.23e-95 288
Msa0995520 MtrunA17_Chr6g0466021 66.162 198 67 0 1 198 4 201 2.44e-91 278
Msa0995520 MtrunA17_Chr4g0048921 32.039 515 337 6 38 548 64 569 8.71e-86 276
Msa0995520 MtrunA17_Chr4g0048911 30.769 546 366 9 2 542 18 556 7.63e-85 274
Msa0995520 MtrunA17_Chr7g0237021 68.421 171 54 0 379 549 1 171 1.32e-83 257
Msa0995520 MtrunA17_Chr7g0236941 68.421 171 54 0 379 549 1 171 1.32e-83 257
Msa0995520 MtrunA17_Chr6g0465941 66.082 171 58 0 379 549 1 171 6.07e-79 245
Msa0995520 MtrunA17_Chr6g0466121 66.082 171 58 0 379 549 1 171 6.07e-79 245
Msa0995520 MtrunA17_Chr6g0465991 66.082 171 58 0 379 549 1 171 6.07e-79 245
Msa0995520 MtrunA17_Chr6g0466091 66.082 171 58 0 379 549 1 171 6.07e-79 245
Msa0995520 MtrunA17_Chr6g0465881 65.497 171 59 0 379 549 1 171 3.45e-78 243
Msa0995520 MtrunA17_Chr2g0280581 29.658 526 341 10 36 541 2 518 5.54e-76 249
Msa0995520 MtrunA17_Chr2g0319081 40.123 324 175 7 228 548 118 425 2.20e-75 245
Msa0995520 MtrunA17_Chr2g0319081 40.164 122 72 1 9 129 19 140 8.38e-23 101
Msa0995520 MtrunA17_Chr2g0310401 32.944 428 284 2 124 549 5 431 1.15e-73 241
Msa0995520 MtrunA17_Chr5g0445501 60.819 171 67 0 379 549 1 171 3.34e-72 228
Msa0995520 MtrunA17_Chr3g0109001 29.980 497 322 9 48 528 13 499 4.74e-70 234
Msa0995520 MtrunA17_Chr3g0109021 29.724 508 331 9 36 528 2 498 1.24e-69 233
Msa0995520 MtrunA17_Chr3g0108941 29.240 513 335 10 48 544 13 513 1.27e-69 233
Msa0995520 MtrunA17_Chr7g0236921 64.331 157 52 1 397 549 1 157 1.59e-69 220
Msa0995520 MtrunA17_Chr3g0109031 29.084 502 342 8 36 528 2 498 4.68e-69 231
Msa0995520 MtrunA17_Chr6g0477111 30.146 481 312 8 67 532 6 477 1.03e-68 229
Msa0995520 MtrunA17_Chr3g0105691 29.283 502 341 7 36 528 35 531 3.98e-68 229
Msa0995520 MtrunA17_Chr3g0108991 29.331 508 333 9 36 528 2 498 1.78e-67 227
Msa0995520 MtrunA17_Chr3g0108951 29.331 508 333 9 36 528 2 498 1.78e-67 227
Msa0995520 MtrunA17_Chr3g0108981 29.560 477 313 6 67 528 6 474 2.22e-66 223
Msa0995520 MtrunA17_Chr3g0108961 29.560 477 313 6 67 528 6 474 2.22e-66 223
Msa0995520 MtrunA17_Chr3g0108971 29.769 477 311 8 67 528 6 473 2.86e-65 220
Msa0995520 MtrunA17_Chr7g0236931 62.745 153 54 1 397 549 1 150 3.06e-65 209
Msa0995520 MtrunA17_Chr3g0109011 29.560 477 312 8 67 528 6 473 4.04e-65 220
Msa0995520 MtrunA17_Chr3g0099031 29.412 510 337 9 48 544 13 512 4.13e-64 218
Msa0995520 MtrunA17_Chr6g0477121 29.024 410 280 6 127 528 10 416 5.89e-56 194
Msa0995520 MtrunA17_Chr7g0233861 63.704 135 40 1 190 324 33 158 2.20e-52 176
Msa0995520 MtrunA17_Chr7g0236981 60.465 129 51 0 1 129 4 132 7.75e-52 173
Msa0995520 MtrunA17_Chr7g0217361 33.333 300 199 1 249 547 8 307 9.76e-51 176
Msa0995520 MtrunA17_Chr8g0357221 37.143 245 145 4 3 245 47 284 7.60e-43 154
Msa0995520 MtrunA17_Chr5g0445401 72.632 95 22 2 160 251 1 94 1.12e-35 128
Msa0995520 MtrunA17_Chr8g0357231 30.116 259 179 2 292 549 1 258 2.38e-34 130
Msa0995520 MtrunA17_Chr5g0445521 66.265 83 28 0 6 88 12 94 9.20e-33 121
Msa0995520 MtrunA17_Chr3g0101681 25.819 519 337 17 64 548 276 780 9.96e-33 133
Msa0995520 MtrunA17_Chr2g0310121 24.225 516 349 15 64 548 275 779 1.14e-32 133
Msa0995520 MtrunA17_Chr3g0082711 47.826 115 43 1 435 549 1 98 6.42e-32 118
Msa0995520 MtrunA17_Chr7g0259881 28.996 269 161 7 67 307 232 498 3.48e-26 113
Msa0995520 MtrunA17_Chr7g0218001 30.741 270 153 7 67 304 349 616 1.26e-24 108
Msa0995520 MtrunA17_Chr7g0217351 29.493 217 144 5 40 252 62 273 6.99e-22 96.3
Msa0995520 MtrunA17_Chr2g0281651 26.087 276 195 6 227 499 43 312 3.80e-19 90.1
Msa0995520 MtrunA17_Chr7g0218041 31.667 240 137 7 114 328 3 240 2.48e-17 84.3
Msa0995520 MtrunA17_Chr5g0411111 38.889 126 62 5 114 228 3 124 5.38e-14 68.9
Msa0995520 MtrunA17_Chr2g0310411 32.000 125 75 4 2 125 49 164 9.99e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0995520 AT5G23960.2 39.927 546 321 5 7 549 3 544 1.30e-150 443
Msa0995520 AT5G23960.1 39.964 548 320 6 7 549 3 546 1.43e-149 441
Msa0995520 AT1G70080.1 38.278 546 327 7 11 548 66 609 8.87e-127 384
Msa0995520 AT3G14490.1 37.912 546 327 7 11 549 60 600 5.83e-122 372
Msa0995520 AT3G14520.1 36.735 539 333 5 16 549 68 603 6.69e-122 372
Msa0995520 AT1G31950.1 36.111 540 335 5 16 549 70 605 2.62e-120 367
Msa0995520 AT3G14540.1 36.920 539 332 4 16 549 65 600 6.44e-120 366
Msa0995520 AT1G31950.3 36.044 541 335 6 16 549 70 606 9.39e-120 366
Msa0995520 AT5G44630.1 35.963 545 338 6 12 548 14 555 1.78e-114 351
Msa0995520 AT1G33750.1 34.831 534 342 4 18 549 73 602 1.30e-113 350
Msa0995520 AT3G32030.1 35.065 539 342 6 16 549 68 603 3.48e-113 349
Msa0995520 AT3G29410.1 34.991 543 345 6 11 549 64 602 9.60e-112 345
Msa0995520 AT4G13300.1 36.872 537 328 7 19 548 20 552 1.72e-111 343
Msa0995520 AT4G20210.1 34.799 546 343 7 11 548 58 598 4.65e-111 343
Msa0995520 AT4G15870.1 34.838 554 335 9 3 548 61 596 4.94e-108 335
Msa0995520 AT3G29190.1 34.579 535 324 9 32 549 75 600 1.74e-107 334
Msa0995520 AT4G20200.1 35.714 518 315 9 42 548 92 602 2.44e-107 334
Msa0995520 AT3G29410.2 35.200 500 318 4 53 549 1 497 9.82e-106 327
Msa0995520 AT4G13280.2 36.075 535 331 7 21 548 22 552 3.23e-105 327
Msa0995520 AT4G20230.1 33.641 541 337 9 19 546 70 601 1.61e-100 316
Msa0995520 AT4G20230.2 33.641 541 337 9 19 546 82 613 2.40e-100 316
Msa0995520 AT3G14540.2 37.444 446 272 3 16 457 65 507 3.94e-100 312
Msa0995520 AT1G31950.2 36.105 457 281 6 16 465 70 522 1.00e-99 312
Msa0995520 AT4G16730.1 31.793 541 352 7 22 549 2 538 6.43e-99 310
Msa0995520 AT3G29110.1 34.679 545 345 9 11 548 55 595 4.30e-98 310
Msa0995520 AT2G23230.2 33.700 546 347 9 10 545 55 595 7.46e-97 307
Msa0995520 AT4G16730.2 32.323 495 320 5 67 549 1 492 2.71e-95 299
Msa0995520 AT1G66020.1 32.537 544 358 6 11 548 56 596 1.06e-94 301
Msa0995520 AT2G23230.1 33.516 546 343 10 10 545 55 590 3.78e-94 300
Msa0995520 AT4G13280.1 34.953 535 307 9 21 548 22 522 5.19e-94 297
Msa0995520 AT1G48800.1 31.319 546 359 9 11 546 63 602 7.62e-92 293
Msa0995520 AT4G16740.1 31.351 555 358 10 6 549 21 563 8.18e-90 287
Msa0995520 AT2G24210.1 31.362 558 364 9 4 549 39 589 1.12e-89 288
Msa0995520 AT3G25820.1 31.362 558 357 12 9 549 45 593 5.97e-87 281
Msa0995520 AT3G25830.1 31.362 558 357 12 9 549 45 593 5.97e-87 281
Msa0995520 AT1G61680.1 31.687 486 322 3 48 528 77 557 7.96e-87 280
Msa0995520 AT3G25810.1 32.937 504 317 9 62 549 98 596 1.13e-86 280
Msa0995520 AT1G61680.2 31.276 486 317 4 48 528 77 550 1.72e-83 271
Msa0995520 AT5G48110.1 37.647 340 207 5 210 548 2 337 2.15e-83 263
Msa0995520 AT1G48820.1 30.983 539 322 11 11 543 63 557 1.03e-80 263
Msa0995520 AT4G16740.2 34.933 375 225 7 6 370 21 386 1.14e-69 229
Msa0995520 AT1G66020.2 34.688 369 232 6 11 373 56 421 9.06e-66 220
Msa0995520 AT3G25820.2 32.642 386 241 8 9 383 45 422 3.87e-65 219
Msa0995520 AT4G16740.4 35.562 329 194 6 6 324 21 341 1.69e-61 207
Msa0995520 AT4G16740.3 35.562 329 194 6 6 324 21 341 1.69e-61 207
Msa0995520 AT3G25820.4 33.945 327 197 8 9 324 45 363 7.31e-56 193
Msa0995520 AT3G25820.3 33.945 327 197 8 9 324 45 363 7.31e-56 193
Msa0995520 AT1G61120.1 24.319 514 345 13 62 548 286 782 1.14e-29 124
Msa0995520 AT1G79460.1 23.314 519 349 19 64 548 274 777 1.97e-26 114
Msa0995520 AT4G02780.1 30.469 256 148 7 67 294 334 587 4.18e-23 104

Find 92 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACTTACGTTGGCAAAAT+TGG 0.135143 6_4:-71863935 Msa0995520:CDS
ATTCTATTTCTGTGCTATTT+AGG 0.140011 6_4:-71864922 Msa0995520:CDS
TTCTATTTCTGTGCTATTTA+GGG 0.183039 6_4:-71864921 Msa0995520:CDS
AAAAGAAAATTGACAATTCT+TGG 0.220621 6_4:-71862020 Msa0995520:CDS
CTGCTGTTCATTGCAGGCTT+AGG 0.241228 6_4:-71862422 Msa0995520:CDS
AGCCTACATCAAAAGGAATT+TGG 0.257080 6_4:-71863895 Msa0995520:CDS
ACGTAAGTAGAATTTCATTA+TGG 0.259315 6_4:+71863947 None:intergenic
GATTCATGGTGAGAAAATTT+TGG 0.259321 6_4:-71864133 Msa0995520:CDS
TCCGTATGCTTCAGAATCTT+TGG 0.269105 6_4:-71865935 Msa0995520:intron
GTCATCAATGTATGATAAAA+TGG 0.271835 6_4:+71863625 None:intergenic
ATGCTGCTGCTGTTCATTGC+AGG 0.286868 6_4:-71862428 Msa0995520:CDS
CAGATTTCCACCCTAGCGTT+TGG 0.305039 6_4:-71865972 Msa0995520:CDS
AGATTTCCACCCTAGCGTTT+GGG 0.318717 6_4:-71865971 Msa0995520:CDS
AGACGAAATTAATCAAGTTT+TGG 0.321368 6_4:-71865002 Msa0995520:CDS
TTCCTTGCTTGTCCTTGAAT+TGG 0.334271 6_4:+71864223 None:intergenic
TAGTTGTTTGGATGATCTTC+CGG 0.341349 6_4:-71863294 Msa0995520:CDS
GAGTAGTAGAATGTTGCTTT+TGG 0.347068 6_4:-71863712 Msa0995520:CDS
TTATATGAGGGAATATGATA+TGG 0.360767 6_4:-71862070 Msa0995520:CDS
CCAATTGAAGATATCATTTG+TGG 0.372281 6_4:+71862476 None:intergenic
TGCTGTTCATTGCAGGCTTA+GGG 0.376798 6_4:-71862421 Msa0995520:CDS
CCACAAATGATATCTTCAAT+TGG 0.377027 6_4:-71862476 Msa0995520:CDS
TTCATGTATTCCTCTGTTGT+TGG 0.377218 6_4:+71862567 None:intergenic
CGTAAGTAGAATTTCATTAT+GGG 0.378944 6_4:+71863948 None:intergenic
AAACGTAATGGAACTTTAGT+TGG 0.382677 6_4:+71861970 None:intergenic
TAGGTGGGATATTAGTTGTT+TGG 0.387739 6_4:-71863306 Msa0995520:CDS
GGATGAATCTTGCTCATAGA+TGG 0.390050 6_4:+71863969 None:intergenic
GATTGACTCAATATGTCGTT+TGG 0.390339 6_4:-71865044 Msa0995520:CDS
CATACTTGGAATGTTATATG+AGG 0.398446 6_4:-71862083 Msa0995520:CDS
AGGACATGTTGCTTCATACT+TGG 0.399041 6_4:-71862097 Msa0995520:CDS
ATATAAATGAGGAATGTCTT+AGG 0.410838 6_4:-71861993 Msa0995520:CDS
TCGCAAGAGAAGGACTCAAT+TGG 0.412353 6_4:+71864064 None:intergenic
TAGAGAAACTTCATGTAATC+CGG 0.427919 6_4:+71863275 None:intergenic
GCAAATTGATAAACTAAAAC+AGG 0.437343 6_4:-71865122 Msa0995520:CDS
GTTTCTCTATAAACCACTCT+TGG 0.439981 6_4:-71863261 Msa0995520:CDS
GCTCCAAAGCCTACATCAAA+AGG 0.450495 6_4:-71863902 Msa0995520:CDS
TACGACTTCTTACATTGGAA+TGG 0.454510 6_4:-71862508 Msa0995520:CDS
CACAAATGATATCTTCAATT+GGG 0.456797 6_4:-71862475 Msa0995520:CDS
ACTACTCTGTCCCGTGCATA+AGG 0.457542 6_4:+71863728 None:intergenic
ACGACTTCTTACATTGGAAT+GGG 0.460983 6_4:-71862507 Msa0995520:CDS
TTCCTTGCTTGACAATATTG+AGG 0.465049 6_4:+71863668 None:intergenic
GCCTCACATATGATGATTCA+TGG 0.465433 6_4:-71864147 Msa0995520:CDS
ATTGACTCAATATGTCGTTT+GGG 0.473862 6_4:-71865043 Msa0995520:CDS
ACCAAAATCAATGCTCATGT+TGG 0.476318 6_4:+71861826 None:intergenic
AGATGTATTCAACCAATTCA+AGG 0.477088 6_4:-71864235 Msa0995520:CDS
GAGCAAGAAGTGAAGAAAGA+AGG 0.477567 6_4:-71863221 Msa0995520:CDS
CGCAAGAGAAGGACTCAATT+GGG 0.484801 6_4:+71864065 None:intergenic
CCAAAATCAATGCTCATGTT+GGG 0.485248 6_4:+71861827 None:intergenic
AACATTGATCATAGATGTTG+AGG 0.512550 6_4:-71864190 Msa0995520:CDS
ATCAATGCTCATGTTGGGAT+GGG 0.522554 6_4:+71861832 None:intergenic
TAAGAAGTCGTAGCCAACAC+TGG 0.522936 6_4:+71862519 None:intergenic
CATGGTCTTTACGTTTCACC+TGG 0.523602 6_4:-71864887 Msa0995520:intron
ATATAGGTGGTGGAAGGAGT+TGG 0.524102 6_4:-71863768 Msa0995520:intron
AGCCTCAATATTGTCAAGCA+AGG 0.528176 6_4:-71863670 Msa0995520:CDS
CTGTGCTATTTAGGGTGTTG+AGG 0.528533 6_4:-71864913 Msa0995520:CDS
AATCAATGCTCATGTTGGGA+TGG 0.533171 6_4:+71861831 None:intergenic
TGGCCTCACTCATGTAAGCT+TGG 0.536408 6_4:+71862613 None:intergenic
GATTTCCACCCTAGCGTTTG+GGG 0.542721 6_4:-71865970 Msa0995520:CDS
TTGTCAAGCAAGGAAAATGA+TGG 0.545763 6_4:-71863660 Msa0995520:CDS
TAGCTTCTTGCCTCTAGCCT+AGG 0.554871 6_4:+71863997 None:intergenic
ATACTTGGAATGTTATATGA+GGG 0.559157 6_4:-71862082 Msa0995520:CDS
GACAAGCAAATCATTAATGT+AGG 0.560101 6_4:+71861858 None:intergenic
TTGACTTGTGTCGCAAGAGA+AGG 0.560387 6_4:+71864054 None:intergenic
GGAATTTGATATAAATATTG+CGG 0.567112 6_4:-71865146 Msa0995520:CDS
CTTGCGACACAAGTCAAACA+TGG 0.567322 6_4:-71864048 Msa0995520:CDS
TTTAGGGTGTTGAGGCAACA+TGG 0.568035 6_4:-71864905 Msa0995520:CDS
ATCAAGTGGTTACCCAGTGT+TGG 0.570889 6_4:-71862532 Msa0995520:CDS
AATGGAGAAATAACTCTCAA+AGG 0.571243 6_4:-71864953 Msa0995520:CDS
AAGCTTACATGAGTGAGGCC+AGG 0.576657 6_4:-71862611 Msa0995520:CDS
ATAGCACAGAAATAGAATGA+AGG 0.580450 6_4:+71864927 None:intergenic
TTGGCTACGACTTCTTACAT+TGG 0.586299 6_4:-71862513 Msa0995520:CDS
GATGACACGTATGATGCATA+CGG 0.587584 6_4:-71863608 Msa0995520:CDS
ACCAAAGATTCTGAAGCATA+CGG 0.593001 6_4:+71865934 None:intergenic
ATTGGGTTGCAATTGACTCA+AGG 0.594526 6_4:+71864082 None:intergenic
AACACATCAACAATATCAAG+TGG 0.595383 6_4:-71862546 Msa0995520:CDS
TCTTCATAAACATCCAAGAG+TGG 0.597368 6_4:+71863248 None:intergenic
ACATTGATCATAGATGTTGA+GGG 0.599537 6_4:-71864189 Msa0995520:CDS
GACAACTACACACATTCTGA+AGG 0.603730 6_4:-71861892 Msa0995520:CDS
TTTCCACCCTAGCGTTTGGG+GGG 0.604138 6_4:-71865968 Msa0995520:CDS
TAATGAAATTCTACTTACGT+TGG 0.604203 6_4:-71863944 Msa0995520:CDS
AACCAATTCAAGGACAAGCA+AGG 0.604565 6_4:-71864225 Msa0995520:CDS
AAGAAGTCGTAGCCAACACT+GGG 0.613680 6_4:+71862520 None:intergenic
CTTTATATATAGGTGGTGGA+AGG 0.614852 6_4:-71863774 Msa0995520:intron
ATTTCCACCCTAGCGTTTGG+GGG 0.619288 6_4:-71865969 Msa0995520:CDS
AAACCTCAATAAAACGCGAA+AGG 0.623927 6_4:+71861929 None:intergenic
TTCTTGGAAAGATATAAATG+AGG 0.632885 6_4:-71862004 Msa0995520:CDS
ACCATGAATCATCATATGTG+AGG 0.641340 6_4:+71864146 None:intergenic
ATAGTTAACATTCAACCACC+TGG 0.654774 6_4:+71862593 None:intergenic
GGATGAAATTGTGTCTAGCG+AGG 0.663791 6_4:-71862400 Msa0995520:intron
AGTCCAAGCTTACATGAGTG+AGG 0.665674 6_4:-71862616 Msa0995520:CDS
AATTGATAAACTAAAACAGG+AGG 0.674763 6_4:-71865119 Msa0995520:CDS
CTTACATGAGTGAGGCCAGG+TGG 0.678940 6_4:-71862608 Msa0995520:CDS
TAACTATATACCAACAACAG+AGG 0.696944 6_4:-71862577 Msa0995520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATAATTAAAGATATTAA+CGG - chr6_4:71862185-71862204 Msa0995520:intron 10.0%
!! TTGAAAATATATATATTACA+AGG + chr6_4:71862578-71862597 None:intergenic 10.0%
!!! ATTATATTTTTGATATCATT+AGG - chr6_4:71863404-71863423 Msa0995520:intron 10.0%
!!! ATTTAATTTTAAATTAAGGA+GGG + chr6_4:71864013-71864032 None:intergenic 10.0%
!!! AATTTAATTTTAAATTAAGG+AGG + chr6_4:71864014-71864033 None:intergenic 10.0%
!!! ATTAAATAATAACTTTTAGA+TGG - chr6_4:71864406-71864425 Msa0995520:intron 10.0%
!!! TTTTATCATATATTTATACA+CGG - chr6_4:71864470-71864489 Msa0995520:intron 10.0%
!!! TTATAATGAAAATGAATTTT+AGG - chr6_4:71864527-71864546 Msa0995520:intron 10.0%
!! TTTAAAAGAAGATTATATAT+TGG - chr6_4:71865071-71865090 Msa0995520:CDS 10.0%
!! TATATTATGAAAAGTGATTA+AGG - chr6_4:71862276-71862295 Msa0995520:intron 15.0%
!! GAATCATTAATATTAATAAG+TGG + chr6_4:71863002-71863021 None:intergenic 15.0%
!!! TAATTTTTTAACTTTGATCA+TGG - chr6_4:71863133-71863152 Msa0995520:intron 15.0%
!!! AATTTTTTAACTTTGATCAT+GGG - chr6_4:71863134-71863153 Msa0995520:intron 15.0%
!!! TAATATAAACCTCTAAAAAA+TGG - chr6_4:71863164-71863183 Msa0995520:intron 15.0%
!!! ATGATTGATTTACATAAAAT+AGG - chr6_4:71863292-71863311 Msa0995520:CDS 15.0%
!! ATAAAGACCAAAAATATTAT+AGG + chr6_4:71864064-71864083 None:intergenic 15.0%
!!! TATTTTTGGTCTTTATATAT+AGG - chr6_4:71864068-71864087 Msa0995520:CDS 15.0%
!!! TGTTATCAGAATAAAATTTT+AGG - chr6_4:71864779-71864798 Msa0995520:intron 15.0%
!! AATATAGTAGAATTGTTAAT+AGG + chr6_4:71865190-71865209 None:intergenic 15.0%
!! TATTATATTAACTCTTAAGA+CGG + chr6_4:71865616-71865635 None:intergenic 15.0%
!! AATATAATAAGATGACATAA+TGG - chr6_4:71865627-71865646 Msa0995520:intron 15.0%
!! TGTTCACGTAAAAAAAAAAA+GGG + chr6_4:71861956-71861975 None:intergenic 20.0%
!! TTGTTCACGTAAAAAAAAAA+AGG + chr6_4:71861957-71861976 None:intergenic 20.0%
!! AAATAAAACCAAGAGAAAAT+AGG - chr6_4:71862336-71862355 Msa0995520:intron 20.0%
!! ATGTGTAATAATATCTATGT+TGG + chr6_4:71862462-71862481 None:intergenic 20.0%
!!! ACACTTAATTGTTATTTGAA+AGG + chr6_4:71862638-71862657 None:intergenic 20.0%
!! GGAATTTGATATAAATATTG+CGG - chr6_4:71862706-71862725 Msa0995520:intron 20.0%
!!! ATTTTTTAACTTTGATCATG+GGG - chr6_4:71863135-71863154 Msa0995520:intron 20.0%
!!! ATACTTAAACCATTTTTTAG+AGG + chr6_4:71863176-71863195 None:intergenic 20.0%
!! TTGATTTACATAAAATAGGA+AGG - chr6_4:71863296-71863315 Msa0995520:CDS 20.0%
!! AATTAAGATAATGTATAGTG+TGG + chr6_4:71863488-71863507 None:intergenic 20.0%
!!! CTAATTGCCTATAATATTTT+TGG - chr6_4:71864054-71864073 Msa0995520:CDS 20.0%
!!! GTTTTTCTATCTCTAAATTT+TGG + chr6_4:71864341-71864360 None:intergenic 20.0%
!!! TAATGAAAATGAATTTTAGG+TGG - chr6_4:71864530-71864549 Msa0995520:intron 20.0%
!!! AATGAAAATGAATTTTAGGT+GGG - chr6_4:71864531-71864550 Msa0995520:intron 20.0%
!!! TTTTGTTGAACTATTATCTA+CGG + chr6_4:71865109-71865128 None:intergenic 20.0%
!! CATATTTACCAAAAATACAT+AGG - chr6_4:71865149-71865168 Msa0995520:intron 20.0%
!!! ATTGTTCATTTTTGTAAACA+AGG - chr6_4:71865543-71865562 Msa0995520:intron 20.0%
!!! AAGAATTGTCAATTTTCTTT+TGG + chr6_4:71865833-71865852 None:intergenic 20.0%
!! AAAAGAAAATTGACAATTCT+TGG - chr6_4:71865832-71865851 Msa0995520:intron 20.0%
! GCAATGTTAGTAATATTAAG+TGG + chr6_4:71862522-71862541 None:intergenic 25.0%
! TTCAAGAATGCCAATAATAT+AGG - chr6_4:71862593-71862612 Msa0995520:CDS 25.0%
! GTCAAAAGATCCTATATTAT+TGG + chr6_4:71862606-71862625 None:intergenic 25.0%
!!! TTCATGTTTTGATTACATAC+AGG - chr6_4:71862685-71862704 Msa0995520:intron 25.0%
! GCAAATTGATAAACTAAAAC+AGG - chr6_4:71862730-71862749 Msa0995520:intron 25.0%
! AATTGATAAACTAAAACAGG+AGG - chr6_4:71862733-71862752 Msa0995520:intron 25.0%
!!! ATCAAGTTGACTTTTTTCAA+CGG + chr6_4:71862791-71862810 None:intergenic 25.0%
!! AGACGAAATTAATCAAGTTT+TGG - chr6_4:71862850-71862869 Msa0995520:intron 25.0%
!!! ATTCTATTTCTGTGCTATTT+AGG - chr6_4:71862930-71862949 Msa0995520:intron 25.0%
!!! TTCTATTTCTGTGCTATTTA+GGG - chr6_4:71862931-71862950 Msa0995520:intron 25.0%
! CGTTGTTACTAAAAATGTAA+AGG + chr6_4:71863202-71863221 None:intergenic 25.0%
!!! TATTTTTGATATCATTAGGC+CGG - chr6_4:71863408-71863427 Msa0995520:intron 25.0%
! CGTAAGTAGAATTTCATTAT+GGG + chr6_4:71863907-71863926 None:intergenic 25.0%
! ACGTAAGTAGAATTTCATTA+TGG + chr6_4:71863908-71863927 None:intergenic 25.0%
! TAATGAAATTCTACTTACGT+TGG - chr6_4:71863908-71863927 Msa0995520:CDS 25.0%
!!! TTTTGGTCTTTATATATAGG+TGG - chr6_4:71864071-71864090 Msa0995520:CDS 25.0%
! GTCATCAATGTATGATAAAA+TGG + chr6_4:71864230-71864249 None:intergenic 25.0%
!! TGAGTTAGAACTTTTTACTA+AGG - chr6_4:71864276-71864295 Msa0995520:intron 25.0%
!! TTTTTACTAAGGCAATTGAA+AGG - chr6_4:71864287-71864306 Msa0995520:intron 25.0%
!!! TCTTACTTCTTTTAAGAAGT+GGG + chr6_4:71864674-71864693 None:intergenic 25.0%
! GATCTATGTATGTATCTATA+CGG + chr6_4:71864825-71864844 None:intergenic 25.0%
!! TTGTGATCAGAACATTTTTA+GGG - chr6_4:71864855-71864874 Msa0995520:intron 25.0%
! CACAAATGATATCTTCAATT+GGG - chr6_4:71865377-71865396 Msa0995520:intron 25.0%
! ATACTTGGAATGTTATATGA+GGG - chr6_4:71865770-71865789 Msa0995520:intron 25.0%
! TTATATGAGGGAATATGATA+TGG - chr6_4:71865782-71865801 Msa0995520:intron 25.0%
! TTCTTGGAAAGATATAAATG+AGG - chr6_4:71865848-71865867 Msa0995520:intron 25.0%
! ATATAAATGAGGAATGTCTT+AGG - chr6_4:71865859-71865878 Msa0995520:intron 25.0%
!!! GACATGTATTTTGAAACAGA+GGG + chr6_4:71862018-71862037 None:intergenic 30.0%
ATAGTGCACATTGCAAAAAT+TGG + chr6_4:71862040-71862059 None:intergenic 30.0%
!! CTTGGACATCTATTTTGAAA+CGG - chr6_4:71862243-71862262 Msa0995520:intron 30.0%
! GTTGGTTATTTGTTAGCAAT+AGG - chr6_4:71862385-71862404 Msa0995520:intron 30.0%
! TAGGATTTCCAACATTTTTG+AGG - chr6_4:71862425-71862444 Msa0995520:CDS 30.0%
!! TCCAACATTTTTGAGGAAAA+AGG - chr6_4:71862432-71862451 Msa0995520:CDS 30.0%
! TCCTTTTTCCTCAAAAATGT+TGG + chr6_4:71862436-71862455 None:intergenic 30.0%
TTAATTATACGATCCAAGTG+TGG + chr6_4:71862495-71862514 None:intergenic 30.0%
! ATTGACTCAATATGTCGTTT+GGG - chr6_4:71862809-71862828 Msa0995520:intron 30.0%
! CATTTTGAACAAAATCCTTG+TGG + chr6_4:71862883-71862902 None:intergenic 30.0%
! CACAAGGATTTTGTTCAAAA+TGG - chr6_4:71862881-71862900 Msa0995520:intron 30.0%
AATGGAGAAATAACTCTCAA+AGG - chr6_4:71862899-71862918 Msa0995520:intron 30.0%
ATAGCACAGAAATAGAATGA+AGG + chr6_4:71862928-71862947 None:intergenic 30.0%
TAAGTGGAGATATAAATACC+AGG + chr6_4:71862986-71863005 None:intergenic 30.0%
TTGAGTAAAACAAACTTGTG+TGG - chr6_4:71863050-71863069 Msa0995520:intron 30.0%
!! GGATAATGGGTTGTTTAATA+TGG + chr6_4:71863340-71863359 None:intergenic 30.0%
CATAACATTACACGGATAAT+GGG + chr6_4:71863353-71863372 None:intergenic 30.0%
AAGGTTCTTCCTTAATAAGA+TGG + chr6_4:71863450-71863469 None:intergenic 30.0%
AGATGTATTCAACCAATTCA+AGG - chr6_4:71863617-71863636 Msa0995520:CDS 30.0%
AACATTGATCATAGATGTTG+AGG - chr6_4:71863662-71863681 Msa0995520:CDS 30.0%
ACATTGATCATAGATGTTGA+GGG - chr6_4:71863663-71863682 Msa0995520:CDS 30.0%
! GATTCATGGTGAGAAAATTT+TGG - chr6_4:71863719-71863738 Msa0995520:CDS 30.0%
! TTCCAAATTCCTTTTGATGT+AGG + chr6_4:71863962-71863981 None:intergenic 30.0%
! ATTTTAAATTAAGGAGGGTG+TGG + chr6_4:71864008-71864027 None:intergenic 30.0%
CTAAAAAACTACCTTATGCA+CGG - chr6_4:71864113-71864132 Msa0995520:CDS 30.0%
TAAAAAACTACCTTATGCAC+GGG - chr6_4:71864114-71864133 Msa0995520:CDS 30.0%
!!! AGTAGTAGAATGTTGCTTTT+GGG - chr6_4:71864141-71864160 Msa0995520:CDS 30.0%
TAGAGAAACTTCATGTAATC+CGG + chr6_4:71864580-71864599 None:intergenic 30.0%
!! TTCTTTTAAGAAGTGGGTAA+TGG + chr6_4:71864668-71864687 None:intergenic 30.0%
!! CTCTTACTTCTTTTAAGAAG+TGG + chr6_4:71864675-71864694 None:intergenic 30.0%
! CTTGTGATCAGAACATTTTT+AGG - chr6_4:71864854-71864873 Msa0995520:intron 30.0%
!! TGTGATCAGAACATTTTTAG+GGG - chr6_4:71864856-71864875 Msa0995520:intron 30.0%
TAACTATATACCAACAACAG+AGG - chr6_4:71865275-71865294 Msa0995520:intron 30.0%
AACACATCAACAATATCAAG+TGG - chr6_4:71865306-71865325 Msa0995520:intron 30.0%
CCAATTGAAGATATCATTTG+TGG + chr6_4:71865379-71865398 None:intergenic 30.0%
CCACAAATGATATCTTCAAT+TGG - chr6_4:71865376-71865395 Msa0995520:intron 30.0%
ATTTCACGCAATATTCTTTG+TGG - chr6_4:71865500-71865519 Msa0995520:intron 30.0%
CATACTTGGAATGTTATATG+AGG - chr6_4:71865769-71865788 Msa0995520:intron 30.0%
! TTTCTTTTGGCATTCTTGAA+TGG + chr6_4:71865820-71865839 None:intergenic 30.0%
! AAACGTAATGGAACTTTAGT+TGG + chr6_4:71865885-71865904 None:intergenic 30.0%
GACAAGCAAATCATTAATGT+AGG + chr6_4:71865997-71866016 None:intergenic 30.0%
!! ACCAAAGATTCTGAAGCATA+CGG + chr6_4:71861921-71861940 None:intergenic 35.0%
!!! GGACATGTATTTTGAAACAG+AGG + chr6_4:71862019-71862038 None:intergenic 35.0%
TAACGGTCAAAATTGTGCAT+TGG - chr6_4:71862202-71862221 Msa0995520:intron 35.0%
!!! GACATCTATTTTGAAACGGA+GGG - chr6_4:71862247-71862266 Msa0995520:intron 35.0%
!! TAGCTCTACCTATTTTCTCT+TGG + chr6_4:71862347-71862366 None:intergenic 35.0%
CTAACAAATAACCAACCCTT+TGG + chr6_4:71862381-71862400 None:intergenic 35.0%
TTAGCAATAGGAATGAAACC+TGG - chr6_4:71862397-71862416 Msa0995520:intron 35.0%
! GATTGACTCAATATGTCGTT+TGG - chr6_4:71862808-71862827 Msa0995520:intron 35.0%
!!! AGTTTTGGAAGATATCCACA+AGG - chr6_4:71862865-71862884 Msa0995520:intron 35.0%
AAAATAGGAAGGATCATCGT+AGG - chr6_4:71863307-71863326 Msa0995520:intron 35.0%
GCATAACATTACACGGATAA+TGG + chr6_4:71863354-71863373 None:intergenic 35.0%
GAACAGAGCATAACATTACA+CGG + chr6_4:71863361-71863380 None:intergenic 35.0%
TAATAAGATGGCTACCCTAT+AGG + chr6_4:71863438-71863457 None:intergenic 35.0%
ATAGGGTAGCCATCTTATTA+AGG - chr6_4:71863438-71863457 Msa0995520:intron 35.0%
!! GTGGTTCTAGAGTATGAAAA+AGG + chr6_4:71863469-71863488 None:intergenic 35.0%
ATGATTGTTCAACATTGTCG+TGG - chr6_4:71863576-71863595 Msa0995520:CDS 35.0%
ACCATGAATCATCATATGTG+AGG + chr6_4:71863709-71863728 None:intergenic 35.0%
!! TGGTGAGAAAATTTTGGAAG+AGG - chr6_4:71863725-71863744 Msa0995520:CDS 35.0%
!! GAAAATTTTGGAAGAGGCAT+TGG - chr6_4:71863731-71863750 Msa0995520:CDS 35.0%
TCTACTTACGTTGGCAAAAT+TGG - chr6_4:71863917-71863936 Msa0995520:CDS 35.0%
! ATTCCTTTTGATGTAGGCTT+TGG + chr6_4:71863956-71863975 None:intergenic 35.0%
AGCCTACATCAAAAGGAATT+TGG - chr6_4:71863957-71863976 Msa0995520:CDS 35.0%
TGGTCTTTATATATAGGTGG+TGG - chr6_4:71864074-71864093 Msa0995520:CDS 35.0%
!! CTTTATATATAGGTGGTGGA+AGG - chr6_4:71864078-71864097 Msa0995520:CDS 35.0%
!!! GAGTAGTAGAATGTTGCTTT+TGG - chr6_4:71864140-71864159 Msa0995520:CDS 35.0%
!!! ATGTTGCTTTTGGGTTATGA+CGG - chr6_4:71864150-71864169 Msa0995520:CDS 35.0%
TTCCTTGCTTGACAATATTG+AGG + chr6_4:71864187-71864206 None:intergenic 35.0%
TTGTCAAGCAAGGAAAATGA+TGG - chr6_4:71864192-71864211 Msa0995520:CDS 35.0%
TAGGTGGGATATTAGTTGTT+TGG - chr6_4:71864546-71864565 Msa0995520:intron 35.0%
TAGTTGTTTGGATGATCTTC+CGG - chr6_4:71864558-71864577 Msa0995520:intron 35.0%
GTTTCTCTATAAACCACTCT+TGG - chr6_4:71864591-71864610 Msa0995520:intron 35.0%
TCTTCATAAACATCCAAGAG+TGG + chr6_4:71864607-71864626 None:intergenic 35.0%
! TTCTGATCACAAGATTTGCT+AGG + chr6_4:71864847-71864866 None:intergenic 35.0%
! CCTGAACTCCTATGTATTTT+TGG + chr6_4:71865160-71865179 None:intergenic 35.0%
CCAAAAATACATAGGAGTTC+AGG - chr6_4:71865157-71865176 Msa0995520:intron 35.0%
ATAGTTAACATTCAACCACC+TGG + chr6_4:71865262-71865281 None:intergenic 35.0%
TTCATGTATTCCTCTGTTGT+TGG + chr6_4:71865288-71865307 None:intergenic 35.0%
TACGACTTCTTACATTGGAA+TGG - chr6_4:71865344-71865363 Msa0995520:intron 35.0%
ACGACTTCTTACATTGGAAT+GGG - chr6_4:71865345-71865364 Msa0995520:intron 35.0%
! GCAGCAGCATTAACAATTTT+CGG + chr6_4:71865414-71865433 None:intergenic 35.0%
AAACAGTTCGAGCAAAAAAG+AGG - chr6_4:71865735-71865754 Msa0995520:intron 35.0%
ACACACGTCAAAAAACGTAA+TGG + chr6_4:71865897-71865916 None:intergenic 35.0%
AAACCTCAATAAAACGCGAA+AGG + chr6_4:71865926-71865945 None:intergenic 35.0%
TCCGTATGCTTCAGAATCTT+TGG - chr6_4:71861917-71861936 Msa0995520:CDS 40.0%
CATATGTGAAGTGGTTGACT+TGG - chr6_4:71862225-71862244 Msa0995520:intron 40.0%
!!! GGACATCTATTTTGAAACGG+AGG - chr6_4:71862246-71862265 Msa0995520:intron 40.0%
AGCTATAGCAAACACTCCAA+AGG - chr6_4:71862362-71862381 Msa0995520:intron 40.0%
GCTATAGCAAACACTCCAAA+GGG - chr6_4:71862363-71862382 Msa0995520:intron 40.0%
! GGAATGAAACCTGGTGTTTT+AGG - chr6_4:71862406-71862425 Msa0995520:CDS 40.0%
GTTGGAAATCCTAAAACACC+AGG + chr6_4:71862418-71862437 None:intergenic 40.0%
TTATACGATCCAAGTGTGGT+TGG + chr6_4:71862491-71862510 None:intergenic 40.0%
ACTAACATTGCACACTCCAA+AGG - chr6_4:71862531-71862550 Msa0995520:CDS 40.0%
CTAACATTGCACACTCCAAA+GGG - chr6_4:71862532-71862551 Msa0995520:CDS 40.0%
GTGAGTAACTTGAATCCCTT+TGG + chr6_4:71862550-71862569 None:intergenic 40.0%
! ATTAGGCCGGTTTACCTATA+GGG - chr6_4:71863421-71863440 Msa0995520:intron 40.0%
AACCAATTCAAGGACAAGCA+AGG - chr6_4:71863627-71863646 Msa0995520:CDS 40.0%
TTCCTTGCTTGTCCTTGAAT+TGG + chr6_4:71863632-71863651 None:intergenic 40.0%
GCCTCACATATGATGATTCA+TGG - chr6_4:71863705-71863724 Msa0995520:CDS 40.0%
! ATTGGGTTGCAATTGACTCA+AGG + chr6_4:71863773-71863792 None:intergenic 40.0%
GGATGAATCTTGCTCATAGA+TGG + chr6_4:71863886-71863905 None:intergenic 40.0%
AGCCTCAATATTGTCAAGCA+AGG - chr6_4:71864182-71864201 Msa0995520:CDS 40.0%
GATGACACGTATGATGCATA+CGG - chr6_4:71864244-71864263 Msa0995520:intron 40.0%
GAGCAAGAAGTGAAGAAAGA+AGG - chr6_4:71864631-71864650 Msa0995520:intron 40.0%
GTATGTATCTATACGGAGAG+TGG + chr6_4:71864818-71864837 None:intergenic 40.0%
TTGGCTACGACTTCTTACAT+TGG - chr6_4:71865339-71865358 Msa0995520:intron 40.0%
! AGGACATGTTGCTTCATACT+TGG - chr6_4:71865755-71865774 Msa0995520:intron 40.0%
!! CAACCTTTCGCGTTTTATTG+AGG - chr6_4:71865920-71865939 Msa0995520:intron 40.0%
GACAACTACACACATTCTGA+AGG - chr6_4:71865960-71865979 Msa0995520:CDS 40.0%
AGATTTCCACCCTAGCGTTT+GGG - chr6_4:71861881-71861900 Msa0995520:CDS 45.0%
! GTGCATTGGCATATGTGAAG+TGG - chr6_4:71862216-71862235 Msa0995520:intron 45.0%
TAGCAAACACTCCAAAGGGT+TGG - chr6_4:71862367-71862386 Msa0995520:intron 45.0%
!! CTGTGCTATTTAGGGTGTTG+AGG - chr6_4:71862939-71862958 Msa0995520:intron 45.0%
!! TTTAGGGTGTTGAGGCAACA+TGG - chr6_4:71862947-71862966 Msa0995520:intron 45.0%
CATGGTCTTTACGTTTCACC+TGG - chr6_4:71862965-71862984 Msa0995520:intron 45.0%
AGGAAGGATCATCGTAGGAT+CGG - chr6_4:71863312-71863331 Msa0995520:intron 45.0%
! CATTAGGCCGGTTTACCTAT+AGG - chr6_4:71863420-71863439 Msa0995520:intron 45.0%
TGGCTACCCTATAGGTAAAC+CGG + chr6_4:71863430-71863449 None:intergenic 45.0%
!!! GAGGCATTGGCTTTTACTTC+TGG - chr6_4:71863744-71863763 Msa0995520:CDS 45.0%
CGCAAGAGAAGGACTCAATT+GGG + chr6_4:71863790-71863809 None:intergenic 45.0%
TCGCAAGAGAAGGACTCAAT+TGG + chr6_4:71863791-71863810 None:intergenic 45.0%
!! TTGACTTGTGTCGCAAGAGA+AGG + chr6_4:71863801-71863820 None:intergenic 45.0%
CTTGCGACACAAGTCAAACA+TGG - chr6_4:71863804-71863823 Msa0995520:intron 45.0%
CAAAAACTTGCCTAGGCTAG+AGG - chr6_4:71863845-71863864 Msa0995520:intron 45.0%
GCTCCAAAGCCTACATCAAA+AGG - chr6_4:71863950-71863969 Msa0995520:CDS 45.0%
!! ATATAGGTGGTGGAAGGAGT+TGG - chr6_4:71864084-71864103 Msa0995520:CDS 45.0%
AGTCCAAGCTTACATGAGTG+AGG - chr6_4:71865236-71865255 Msa0995520:intron 45.0%
ATCAAGTGGTTACCCAGTGT+TGG - chr6_4:71865320-71865339 Msa0995520:intron 45.0%
AAGAAGTCGTAGCCAACACT+GGG + chr6_4:71865335-71865354 None:intergenic 45.0%
TAAGAAGTCGTAGCCAACAC+TGG + chr6_4:71865336-71865355 None:intergenic 45.0%
TGCTGTTCATTGCAGGCTTA+GGG - chr6_4:71865431-71865450 Msa0995520:intron 45.0%
GGATGAAATTGTGTCTAGCG+AGG - chr6_4:71865452-71865471 Msa0995520:intron 45.0%
!!! TCATTTTTTATATTTTAATT+TGG - chr6_4:71863020-71863039 Msa0995520:intron 5.0%
!!! TTCAATTTAATTTTAAATTA+AGG + chr6_4:71864017-71864036 None:intergenic 5.0%
CAGATTTCCACCCTAGCGTT+TGG - chr6_4:71861880-71861899 Msa0995520:CDS 50.0%
GATTTCCACCCTAGCGTTTG+GGG - chr6_4:71861882-71861901 Msa0995520:CDS 50.0%
ATTTCCACCCTAGCGTTTGG+GGG - chr6_4:71861883-71861902 Msa0995520:CDS 50.0%
CATTGCACACCAACCACACT+TGG - chr6_4:71862479-71862498 Msa0995520:CDS 50.0%
! CTAGGCAAGTTTTTGCGCAG+AGG + chr6_4:71863840-71863859 None:intergenic 50.0%
CTCTGCGCAAAAACTTGCCT+AGG - chr6_4:71863838-71863857 Msa0995520:intron 50.0%
TAGCTTCTTGCCTCTAGCCT+AGG + chr6_4:71863858-71863877 None:intergenic 50.0%
ACTACTCTGTCCCGTGCATA+AGG + chr6_4:71864127-71864146 None:intergenic 50.0%
TGGCCTCACTCATGTAAGCT+TGG + chr6_4:71865242-71865261 None:intergenic 50.0%
AAGCTTACATGAGTGAGGCC+AGG - chr6_4:71865241-71865260 Msa0995520:intron 50.0%
ATGCTGCTGCTGTTCATTGC+AGG - chr6_4:71865424-71865443 Msa0995520:intron 50.0%
CTGCTGTTCATTGCAGGCTT+AGG - chr6_4:71865430-71865449 Msa0995520:intron 50.0%
TTTCCACCCTAGCGTTTGGG+GGG - chr6_4:71861884-71861903 Msa0995520:CDS 55.0%
AGAAGTCCCCCCAAACGCTA+GGG + chr6_4:71861893-71861912 None:intergenic 55.0%
AAGAAGTCCCCCCAAACGCT+AGG + chr6_4:71861894-71861913 None:intergenic 55.0%
CTTACATGAGTGAGGCCAGG+TGG - chr6_4:71865244-71865263 Msa0995520:intron 55.0%
AGTCCCCCCAAACGCTAGGG+TGG + chr6_4:71861890-71861909 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 71861840 71866034 71861840 ID=Msa0995520;Name=Msa0995520
chr6_4 mRNA 71861840 71866034 71861840 ID=Msa0995520-mRNA-1;Parent=Msa0995520;Name=Msa0995520-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|1|1|7|0|550
chr6_4 exon 71865936 71866034 71865936 ID=Msa0995520-mRNA-1:exon:13418;Parent=Msa0995520-mRNA-1
chr6_4 exon 71864888 71865167 71864888 ID=Msa0995520-mRNA-1:exon:13417;Parent=Msa0995520-mRNA-1
chr6_4 exon 71863883 71864255 71863883 ID=Msa0995520-mRNA-1:exon:13416;Parent=Msa0995520-mRNA-1
chr6_4 exon 71863566 71863784 71863566 ID=Msa0995520-mRNA-1:exon:13415;Parent=Msa0995520-mRNA-1
chr6_4 exon 71863187 71863325 71863187 ID=Msa0995520-mRNA-1:exon:13414;Parent=Msa0995520-mRNA-1
chr6_4 exon 71862401 71862649 71862401 ID=Msa0995520-mRNA-1:exon:13413;Parent=Msa0995520-mRNA-1
chr6_4 exon 71861840 71862133 71861840 ID=Msa0995520-mRNA-1:exon:13412;Parent=Msa0995520-mRNA-1
chr6_4 CDS 71865936 71866034 71865936 ID=Msa0995520-mRNA-1:cds;Parent=Msa0995520-mRNA-1
chr6_4 CDS 71864888 71865167 71864888 ID=Msa0995520-mRNA-1:cds;Parent=Msa0995520-mRNA-1
chr6_4 CDS 71863883 71864255 71863883 ID=Msa0995520-mRNA-1:cds;Parent=Msa0995520-mRNA-1
chr6_4 CDS 71863566 71863784 71863566 ID=Msa0995520-mRNA-1:cds;Parent=Msa0995520-mRNA-1
chr6_4 CDS 71863187 71863325 71863187 ID=Msa0995520-mRNA-1:cds;Parent=Msa0995520-mRNA-1
chr6_4 CDS 71862401 71862649 71862401 ID=Msa0995520-mRNA-1:cds;Parent=Msa0995520-mRNA-1
chr6_4 CDS 71861840 71862133 71861840 ID=Msa0995520-mRNA-1:cds;Parent=Msa0995520-mRNA-1
Gene Sequence

>Msa0995520

ATGTCTCATAACGAGAAATCAGTTTTGCAAAGAAATGTTGCAGATTTCCACCCTAGCGTTTGGGGGGACTTCTTCATTCCGTATGCTTCAGAATCTTTGGAATTTGATATAAATATTGCGGCGCAAATTGATAAACTAAAACAGGAGGTTAGACAAATGCTTGTCTCAAAAATTGAGAATCCGTTGAAAAAAGTCAACTTGATTGACTCAATATGTCGTTTGGGTGTGAGCTATTATTTTGAAGACGAAATTAATCAAGTTTTGGAAGATATCCACAAGGATTTTGTTCAAAATGGAGAAATAACTCTCAAAGGCAACCTTCATTCTATTTCTGTGCTATTTAGGGTGTTGAGGCAACATGGTCTTTACGTTTCACCTGATGTATTCAACCAATTCAAGGACAAGCAAGGAAATTTTAGTGAAACATTGATCATAGATGTTGAGGGCATGTTAAACTTGTATGAAGCCTCACATATGATGATTCATGGTGAGAAAATTTTGGAAGAGGCATTGGCTTTTACTTCTGGTCACCTTGAGTCAATTGCAACCCAATTGAGTCCTTCTCTTGCGACACAAGTCAAACATGGCTTAAGACATCCTCTGCGCAAAAACTTGCCTAGGCTAGAGGCAAGAAGCTACATTTCCATCTATGAGCAAGATTCATCCCATAATGAAATTCTACTTACGTTGGCAAAATTGGATTTTAATATGCTCCAAAGCCTACATCAAAAGGAATTTGGAAACATTTGCAAGTGGTGGAAGGAGTTGGACATTGCTAAAAAACTACCTTATGCACGGGACAGAGTAGTAGAATGTTGCTTTTGGGTTATGACGGTATATTTTGAGCCTCAATATTGTCAAGCAAGGAAAATGATGGCAAAAGTAATCTCCATTTTATCATACATTGATGACACGTATGATGCATACGGAACAATTGATGAGTTAGAACTTTTTACTAAGGCAATTGAAAGGTGGGATATTAGTTGTTTGGATGATCTTCCGGATTACATGAAGTTTCTCTATAAACCACTCTTGGATGTTTATGAAGAAATTGAGCAAGAAGTGAAGAAAGAAGGAAGAGTATATGCCATTACCCACTTCTTAAAAGAATTCAAAAATATAGTCCAAGCTTACATGAGTGAGGCCAGGTGGTTGAATGTTAACTATATACCAACAACAGAGGAATACATGAACACATCAACAATATCAAGTGGTTACCCAGTGTTGGCTACGACTTCTTACATTGGAATGGGAAACATAGCCACAAATGATATCTTCAATTGGGTAACTAGTCATCCGAAAATTGTTAATGCTGCTGCTGTTCATTGCAGGCTTAGGGATGAAATTGTGTCTAGCGAGTTCGAGCAAAAAAGAGGACATGTTGCTTCATACTTGGAATGTTATATGAGGGAATATGATATGGATAGAGAAGCTGCCATTCAAGAATGCCAAAAGAAAATTGACAATTCTTGGAAAGATATAAATGAGGAATGTCTTAGGCCAACTAAAGTTCCATTACGTTTTTTGACGTGTGTTCTCAACCTTTCGCGTTTTATTGAGGTTTTTTACAAAGATGAAGACAACTACACACATTCTGAAGGACTAATGAAGACCTACATTAATGATTTGCTTGTCAATCCCATCCCAACATGA
Protein sequence

>Msa0995520

MSHNEKSVLQRNVADFHPSVWGDFFIPYASESLEFDINIAAQIDKLKQEVRQMLVSKIENPLKKVNLIDSICRLGVSYYFEDEINQVLEDIHKDFVQNGEITLKGNLHSISVLFRVLRQHGLYVSPDVFNQFKDKQGNFSETLIIDVEGMLNLYEASHMMIHGEKILEEALAFTSGHLESIATQLSPSLATQVKHGLRHPLRKNLPRLEARSYISIYEQDSSHNEILLTLAKLDFNMLQSLHQKEFGNICKWWKELDIAKKLPYARDRVVECCFWVMTVYFEPQYCQARKMMAKVISILSYIDDTYDAYGTIDELELFTKAIERWDISCLDDLPDYMKFLYKPLLDVYEEIEQEVKKEGRVYAITHFLKEFKNIVQAYMSEARWLNVNYIPTTEEYMNTSTISSGYPVLATTSYIGMGNIATNDIFNWVTSHPKIVNAAAVHCRLRDEIVSSEFEQKRGHVASYLECYMREYDMDREAAIQECQKKIDNSWKDINEECLRPTKVPLRFLTCVLNLSRFIEVFYKDEDNYTHSEGLMKTYINDLLVNPIPT*