AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0998000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0998000 MtrunA17_Chr6g0483521 93.722 223 12 2 1 221 1 223 6.12e-109 312
Msa0998000 MtrunA17_Chr6g0483561 69.362 235 56 6 1 221 1 233 5.98e-73 221
Msa0998000 MtrunA17_Chr6g0483581 67.094 234 63 4 1 221 1 233 5.04e-67 206
Msa0998000 MtrunA17_Chr6g0483511 65.196 204 50 6 1 202 1 185 1.34e-65 201
Msa0998000 MtrunA17_Chr2g0290831 54.667 75 34 0 1 75 1 75 3.28e-19 78.6
Msa0998000 MtrunA17_Chr5g0438551 50.000 92 43 2 3 91 2 93 3.59e-19 80.1
Msa0998000 MtrunA17_Chr3g0120551 48.000 75 39 0 2 76 3 77 9.57e-19 78.6
Msa0998000 MtrunA17_Chr3g0120541 48.148 81 41 1 2 82 3 82 4.71e-17 73.9
Msa0998000 MtrunA17_Chr3g0120531 47.887 71 36 1 2 71 3 73 1.67e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0998000 AT3G20180.3 50.000 82 41 0 1 82 1 82 6.25e-18 77.0
Msa0998000 AT3G20180.2 47.561 82 43 0 1 82 20 101 7.60e-17 74.7
Msa0998000 AT4G05030.2 45.946 74 40 0 1 74 1 74 1.04e-15 69.7
Msa0998000 AT3G20180.4 48.101 79 41 0 4 82 2 80 1.10e-15 70.9
Msa0998000 AT5G48290.1 39.130 92 56 0 1 92 1 92 1.44e-15 72.4
Msa0998000 AT3G20180.1 48.684 76 39 0 7 82 3 78 1.80e-15 70.5
Msa0998000 AT4G05030.1 46.479 71 38 0 4 74 37 107 1.09e-14 68.2
Msa0998000 AT3G07600.1 43.678 87 49 0 1 87 1 87 2.50e-14 68.6
Msa0998000 AT5G48290.3 32.143 112 56 1 1 92 1 112 4.08e-11 60.5
Msa0998000 AT5G48290.2 36.585 82 52 0 11 92 1 82 5.28e-11 59.7

Find 63 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGCAAGGTTCAGTATT+TGG 0.171369 6_4:+78158286 Msa0998000:three_prime_UTR
ATAAATTAGTGATGAAAATT+TGG 0.190467 6_4:+78158452 Msa0998000:three_prime_UTR
TTTGGTGAAGGAGGCTTCTT+AGG 0.245817 6_4:-78158056 None:intergenic
AGTAAGAATAGGGTGTTATT+TGG 0.270687 6_4:+78158604 Msa0998000:three_prime_UTR
TGGAGCAAGGTTCAGGATTT+AGG 0.283843 6_4:-78158187 None:intergenic
TCATAACAAGCCGCATAATT+AGG 0.292030 6_4:+78158310 Msa0998000:three_prime_UTR
TCATAACAAACCACATAATT+AGG 0.295551 6_4:-78158164 None:intergenic
CATAATTAGGTGGTGGATAA+AGG 0.302059 6_4:+78158323 Msa0998000:three_prime_UTR
TTCGTATTTGTCTTCTGCTT+TGG 0.306809 6_4:-78156129 None:intergenic
TTACACTCACAACAACTCTT+TGG 0.313053 6_4:-78157984 None:intergenic
AAATTTAGTGATTAAAGAAT+TGG 0.317850 6_4:+78158516 Msa0998000:three_prime_UTR
TCACAACAACTCTTTGGATT+AGG 0.335774 6_4:-78158008 None:intergenic
TTGTTGTCACAACAACTCTT+TGG 0.338124 6_4:-78158014 None:intergenic
GGTGGATAAGGGTTACAATA+AGG 0.360536 6_4:-78158140 None:intergenic
TGGAGCAAGGTTCAGTATTT+GGG 0.362526 6_4:+78158287 Msa0998000:three_prime_UTR
GGGTTACAATAAGGTTGATA+TGG 0.366283 6_4:-78158131 None:intergenic
ACATAATTAGGTGGTGGATA+AGG 0.372201 6_4:-78158152 None:intergenic
AGGTTACAATAAGGTTGATA+TGG 0.374740 6_4:+78158343 Msa0998000:three_prime_UTR
CACATGATGGAGCAAGGTTC+AGG 0.380976 6_4:-78158194 None:intergenic
CTTCTGCTTTGGAATTGAGC+TGG 0.401133 6_4:-78156118 None:intergenic
CATAATTAGGTGGTGGATAA+GGG 0.408424 6_4:-78158151 None:intergenic
GGTGGATAAAGGTTACAATA+AGG 0.411670 6_4:+78158334 Msa0998000:three_prime_UTR
TCATCTCAACAAGTAAGAAT+AGG 0.416800 6_4:+78158593 Msa0998000:three_prime_UTR
GATAGTTATAAAGTTGCAAA+TGG 0.417360 6_4:+78156374 Msa0998000:CDS
TTCAATAAAATTTGCAAGCA+TGG 0.428519 6_4:+78158232 Msa0998000:three_prime_UTR
TTCAATAAAATTTGCAAGCA+TGG 0.428519 6_4:+78158383 Msa0998000:three_prime_UTR
CACCTCTTCCACACTTAGAA+TGG 0.439561 6_4:-78157751 None:intergenic
CATCTCAACAAGTAAGAATA+GGG 0.448825 6_4:+78158594 Msa0998000:three_prime_UTR
AGGTGTGACTTCAGTATCAT+TGG 0.469236 6_4:+78157635 Msa0998000:intron
TTATCCACCACCTAATTATG+TGG 0.470527 6_4:+78158154 Msa0998000:CDS
TGCACATGCCCAAGGTGTTA+TGG 0.479494 6_4:+78158101 Msa0998000:CDS
TGCACCATTGAGGACATTGT+TGG 0.498194 6_4:-78158082 None:intergenic
TCTTGCAATACTCATTGTAA+TGG 0.500068 6_4:+78157882 Msa0998000:CDS
CACCATTCTAAGTGTGGAAG+AGG 0.501486 6_4:+78157749 Msa0998000:CDS
TTTCAATTAAGCTAGGATGA+AGG 0.501682 6_4:+78156210 Msa0998000:exon
TTGTTTGTTTCAATTAAGCT+AGG 0.504012 6_4:+78156203 Msa0998000:five_prime_UTR
GCAGTTTCATAAAATTTACA+TGG 0.530325 6_4:-78158644 None:intergenic
AAATCCAACAATGTCCTCAA+TGG 0.532773 6_4:+78158078 Msa0998000:CDS
GAGCATAACCATAACACCTT+GGG 0.533352 6_4:-78158109 None:intergenic
ATACTTGTATACAATAATGA+TGG 0.534366 6_4:+78158267 Msa0998000:three_prime_UTR
GAATTGAGCTGGTTGTACAT+AGG 0.539972 6_4:-78156107 None:intergenic
CATAGTTCTTACTCACATGA+TGG 0.542275 6_4:-78158207 None:intergenic
CTTGGGCATGTGCACCATTG+AGG 0.562407 6_4:-78158092 None:intergenic
AACTGTGTCTACATTATCAC+CGG 0.572592 6_4:-78157688 None:intergenic
TTATCCACCACCTAATTATG+CGG 0.579927 6_4:-78158320 None:intergenic
AGCACTCAAAACTGCTGCTG+AGG 0.581320 6_4:+78156419 Msa0998000:CDS
AAAACTGCTGCTGAGGTCAA+AGG 0.586105 6_4:+78156426 Msa0998000:CDS
GGAGCATAACCATAACACCT+TGG 0.600046 6_4:-78158110 None:intergenic
CAAACCACATAATTAGGTGG+TGG 0.606455 6_4:-78158158 None:intergenic
ATAGGTTATGATATATACAT+TGG 0.611263 6_4:-78156089 None:intergenic
CTTACTCACATGATGGAGCA+AGG 0.612606 6_4:-78158200 None:intergenic
CAAGCCGCATAATTAGGTGG+TGG 0.615712 6_4:+78158316 Msa0998000:three_prime_UTR
CTCAATGGTGCACATGCCCA+AGG 0.619335 6_4:+78158093 Msa0998000:CDS
CTGTGTCACCATTCTAAGTG+TGG 0.620030 6_4:+78157743 Msa0998000:CDS
GTGACTTCAGTATCATTGGA+AGG 0.626567 6_4:+78157639 Msa0998000:CDS
AAAGATAAGATATCTGTCAC+CGG 0.627820 6_4:+78157669 Msa0998000:CDS
TATACAATAATGATGGAGCA+AGG 0.631525 6_4:+78158274 Msa0998000:three_prime_UTR
TAACAAACCACATAATTAGG+TGG 0.634252 6_4:-78158161 None:intergenic
TTAGTGATGAAAATTTGGAG+AGG 0.642063 6_4:+78158457 Msa0998000:three_prime_UTR
TAACAAGCCGCATAATTAGG+TGG 0.668059 6_4:+78158313 Msa0998000:three_prime_UTR
TGTGGAAGTGCAAAATGTGA+TGG 0.668591 6_4:+78157924 Msa0998000:CDS
TGTGACAACAAGAAGTGTGA+TGG 0.683170 6_4:+78158026 Msa0998000:CDS
AGAGGTGAAGAAGAAAACCG+AGG 0.721257 6_4:+78157767 Msa0998000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGAAATACTAATAAAA+AGG - chr6_4:78156285-78156304 None:intergenic 10.0%
!! ATTAAAATAAATATAGTGAT+TGG - chr6_4:78156464-78156483 None:intergenic 10.0%
!!! CACTATATTTATTTTAATTA+TGG + chr6_4:78156466-78156485 Msa0998000:intron 10.0%
!! TTAATTATGGATTTAAATTA+TGG + chr6_4:78156479-78156498 Msa0998000:intron 10.0%
!! TAATTATGGATTTAAATTAT+GGG + chr6_4:78156480-78156499 Msa0998000:intron 10.0%
!! AAACAAAAAAAATATTTAAG+GGG + chr6_4:78156675-78156694 Msa0998000:intron 10.0%
!!! TTAGAAATTAATTTTATACT+AGG - chr6_4:78156937-78156956 None:intergenic 10.0%
!!! AATTTTTTCTAATGATTATT+TGG - chr6_4:78157211-78157230 None:intergenic 10.0%
!! CTAACAAACTATTTATAAAA+TGG + chr6_4:78156337-78156356 Msa0998000:intron 15.0%
!!! TTTTTTTGGTTGTATATAAA+AGG + chr6_4:78156802-78156821 Msa0998000:intron 15.0%
!!! TTGTATACAATAATCTTTTA+AGG + chr6_4:78158422-78158441 Msa0998000:three_prime_UTR 15.0%
!! ATAAATTAGTGATGAAAATT+TGG + chr6_4:78158452-78158471 Msa0998000:three_prime_UTR 15.0%
!! AAATTTAGTGATTAAAGAAT+TGG + chr6_4:78158516-78158535 Msa0998000:three_prime_UTR 15.0%
!! ATAGGTTATGATATATACAT+TGG - chr6_4:78156092-78156111 None:intergenic 20.0%
!! ACAAAATAATTAAGGAAAAC+CGG - chr6_4:78156702-78156721 None:intergenic 20.0%
!! AAAATCATGAGATTTATTGA+GGG - chr6_4:78156755-78156774 None:intergenic 20.0%
!! TATGATTTCAATCTTACATA+TGG + chr6_4:78156868-78156887 Msa0998000:intron 20.0%
!! ATGATTTCAATCTTACATAT+GGG + chr6_4:78156869-78156888 Msa0998000:intron 20.0%
!! TGGGTATATATGAAAAAATT+TGG + chr6_4:78156888-78156907 Msa0998000:intron 20.0%
!! ATACTTGTATACAATAATGA+TGG + chr6_4:78158267-78158286 Msa0998000:three_prime_UTR 20.0%
!!! AAATTTTATTGAAGGCATTA+TGG - chr6_4:78158376-78158395 None:intergenic 20.0%
! TCCAAAAAAAAAACTAGTTG+CGG - chr6_4:78155986-78156005 None:intergenic 25.0%
!!! CAACTAGTTTTTTTTTTGGA+AGG + chr6_4:78155986-78156005 Msa0998000:five_prime_UTR 25.0%
!!! GCTTCTTTTATTTTTCTGAT+TGG - chr6_4:78156173-78156192 None:intergenic 25.0%
! TTGTTTGTTTCAATTAAGCT+AGG + chr6_4:78156203-78156222 Msa0998000:five_prime_UTR 25.0%
! GATAGTTATAAAGTTGCAAA+TGG + chr6_4:78156374-78156393 Msa0998000:CDS 25.0%
! AAAAAAATATTTAAGGGGAC+CGG + chr6_4:78156680-78156699 Msa0998000:intron 25.0%
!!! TCCTTAATTATTTTGTTCTC+AGG + chr6_4:78156706-78156725 Msa0998000:intron 25.0%
! ACCTGAGAACAAAATAATTA+AGG - chr6_4:78156710-78156729 None:intergenic 25.0%
! GAAAATCATGAGATTTATTG+AGG - chr6_4:78156756-78156775 None:intergenic 25.0%
! TTTCTAAACAACGACATAAT+TGG + chr6_4:78156950-78156969 Msa0998000:intron 25.0%
! GATTATTTGGTTATGTTCTT+TGG - chr6_4:78157198-78157217 None:intergenic 25.0%
! CGAAAAATTGATGTCAAATA+TGG - chr6_4:78157512-78157531 None:intergenic 25.0%
!!! TTCTTTCTTTTTTTCTTCCT+CGG - chr6_4:78157787-78157806 None:intergenic 25.0%
! AGAAGAAAAGAAAAAGATGA+TGG + chr6_4:78157824-78157843 Msa0998000:CDS 25.0%
! TCATAACAAACCACATAATT+AGG - chr6_4:78158167-78158186 None:intergenic 25.0%
!! ATGCTTGCAAATTTTATTGA+AGG - chr6_4:78158233-78158252 None:intergenic 25.0%
! TTCAATAAAATTTGCAAGCA+TGG + chr6_4:78158232-78158251 Msa0998000:three_prime_UTR 25.0%
!! ATGCTTGCAAATTTTATTGA+AGG - chr6_4:78158233-78158252 None:intergenic 25.0%
! TTCAATAAAATTTGCAAGCA+TGG + chr6_4:78158232-78158251 Msa0998000:three_prime_UTR 25.0%
! GCAGTTTCATAAAATTTACA+TGG - chr6_4:78158647-78158666 None:intergenic 25.0%
!!! ACCGCAACTAGTTTTTTTTT+TGG + chr6_4:78155982-78156001 Msa0998000:five_prime_UTR 30.0%
!! CATTGTCATTGTTTTGTGAA+AGG - chr6_4:78156024-78156043 None:intergenic 30.0%
TTTCAATTAAGCTAGGATGA+AGG + chr6_4:78156210-78156229 Msa0998000:exon 30.0%
ATGCGATCAAAATTGCAATT+GGG - chr6_4:78156561-78156580 None:intergenic 30.0%
AATGCGATCAAAATTGCAAT+TGG - chr6_4:78156562-78156581 None:intergenic 30.0%
TCTAACTACAAAGCAAACTA+TGG - chr6_4:78156642-78156661 None:intergenic 30.0%
TTTGCTTTGTAGTTAGAAGT+AGG + chr6_4:78156645-78156664 Msa0998000:intron 30.0%
!!! GTAAACTCTCACTCTTTTTT+TGG + chr6_4:78156788-78156807 Msa0998000:intron 30.0%
! AGAGGTTATATGAACTTTTG+TGG + chr6_4:78156827-78156846 Msa0998000:intron 30.0%
ACAATCAATCATGTTTGTGT+GGG - chr6_4:78157052-78157071 None:intergenic 30.0%
TAGAGGTAAACACATATGTT+CGG - chr6_4:78157439-78157458 None:intergenic 30.0%
TCCAAAATACATGCATACAT+AGG + chr6_4:78157615-78157634 Msa0998000:intron 30.0%
! ACCTATGTATGCATGTATTT+TGG - chr6_4:78157619-78157638 None:intergenic 30.0%
AAAGATAAGATATCTGTCAC+CGG + chr6_4:78157669-78157688 Msa0998000:CDS 30.0%
!!! GAACTTTTTTTTCAGCATGT+TGG - chr6_4:78157721-78157740 None:intergenic 30.0%
TCTTGCAATACTCATTGTAA+TGG + chr6_4:78157882-78157901 Msa0998000:CDS 30.0%
AAGTGTAAAAAATGCACATG+TGG + chr6_4:78157906-78157925 Msa0998000:CDS 30.0%
TAACAAACCACATAATTAGG+TGG - chr6_4:78158164-78158183 None:intergenic 30.0%
! TATACAATAATGATGGAGCA+AGG + chr6_4:78158274-78158293 Msa0998000:three_prime_UTR 30.0%
!! AGGTTACAATAAGGTTGATA+TGG + chr6_4:78158343-78158362 Msa0998000:three_prime_UTR 30.0%
TTAGTGATGAAAATTTGGAG+AGG + chr6_4:78158457-78158476 Msa0998000:three_prime_UTR 30.0%
TCATCTCAACAAGTAAGAAT+AGG + chr6_4:78158593-78158612 Msa0998000:three_prime_UTR 30.0%
CATCTCAACAAGTAAGAATA+GGG + chr6_4:78158594-78158613 Msa0998000:three_prime_UTR 30.0%
!! AGTAAGAATAGGGTGTTATT+TGG + chr6_4:78158604-78158623 Msa0998000:three_prime_UTR 30.0%
TTCGTATTTGTCTTCTGCTT+TGG - chr6_4:78156132-78156151 None:intergenic 35.0%
GGTTGTATATAAAAGGTGAG+AGG + chr6_4:78156809-78156828 Msa0998000:intron 35.0%
CTAAACAACGACATAATTGG+CGG + chr6_4:78156953-78156972 Msa0998000:intron 35.0%
CACAATCAATCATGTTTGTG+TGG - chr6_4:78157053-78157072 None:intergenic 35.0%
GTAAAGTGAGAGAAAGATAG+AGG - chr6_4:78157456-78157475 None:intergenic 35.0%
AACTGTGTCTACATTATCAC+CGG - chr6_4:78157691-78157710 None:intergenic 35.0%
TTACACTCACAACAACTCTT+TGG - chr6_4:78157987-78158006 None:intergenic 35.0%
TCACAACAACTCTTTGGATT+AGG - chr6_4:78157981-78158000 None:intergenic 35.0%
TTGTTGTCACAACAACTCTT+TGG - chr6_4:78158017-78158036 None:intergenic 35.0%
!!! CATTGTTGGATTTTTGGTGA+AGG - chr6_4:78158071-78158090 None:intergenic 35.0%
! TGAGGACATTGTTGGATTTT+TGG - chr6_4:78158077-78158096 None:intergenic 35.0%
AAATCCAACAATGTCCTCAA+TGG + chr6_4:78158078-78158097 Msa0998000:CDS 35.0%
!! GGGTTACAATAAGGTTGATA+TGG - chr6_4:78158134-78158153 None:intergenic 35.0%
!! CATAATTAGGTGGTGGATAA+GGG - chr6_4:78158323-78158342 None:intergenic 35.0%
!! ACATAATTAGGTGGTGGATA+AGG - chr6_4:78158155-78158174 None:intergenic 35.0%
TTATCCACCACCTAATTATG+TGG + chr6_4:78158154-78158173 Msa0998000:CDS 35.0%
CATAGTTCTTACTCACATGA+TGG - chr6_4:78158210-78158229 None:intergenic 35.0%
TCATAACAAGCCGCATAATT+AGG + chr6_4:78158310-78158329 Msa0998000:three_prime_UTR 35.0%
TTATCCACCACCTAATTATG+CGG - chr6_4:78158154-78158173 None:intergenic 35.0%
!! CATAATTAGGTGGTGGATAA+AGG + chr6_4:78158323-78158342 Msa0998000:three_prime_UTR 35.0%
GGTGGATAAAGGTTACAATA+AGG + chr6_4:78158334-78158353 Msa0998000:three_prime_UTR 35.0%
GAATTGAGCTGGTTGTACAT+AGG - chr6_4:78156110-78156129 None:intergenic 40.0%
CTTCAAAATCCGCAATATCG+TGG - chr6_4:78156524-78156543 None:intergenic 40.0%
!! GAAGAAGCACTAGCTAAAAC+TGG - chr6_4:78157569-78157588 None:intergenic 40.0%
AGGTGTGACTTCAGTATCAT+TGG + chr6_4:78157635-78157654 Msa0998000:intron 40.0%
GTGACTTCAGTATCATTGGA+AGG + chr6_4:78157639-78157658 Msa0998000:CDS 40.0%
!! GCATGTCGTACTGTTTTGTT+TGG + chr6_4:78157849-78157868 Msa0998000:CDS 40.0%
! TGTGGAAGTGCAAAATGTGA+TGG + chr6_4:78157924-78157943 Msa0998000:CDS 40.0%
! TGTGACAACAAGAAGTGTGA+TGG + chr6_4:78158026-78158045 Msa0998000:CDS 40.0%
!!! TGTTGGATTTTTGGTGAAGG+AGG - chr6_4:78158068-78158087 None:intergenic 40.0%
GAGCATAACCATAACACCTT+GGG - chr6_4:78158112-78158131 None:intergenic 40.0%
GGTGGATAAGGGTTACAATA+AGG - chr6_4:78158143-78158162 None:intergenic 40.0%
CAAACCACATAATTAGGTGG+TGG - chr6_4:78158161-78158180 None:intergenic 40.0%
ATGGAGCAAGGTTCAGTATT+TGG + chr6_4:78158286-78158305 Msa0998000:three_prime_UTR 40.0%
TGGAGCAAGGTTCAGTATTT+GGG + chr6_4:78158287-78158306 Msa0998000:three_prime_UTR 40.0%
TAACAAGCCGCATAATTAGG+TGG + chr6_4:78158313-78158332 Msa0998000:three_prime_UTR 40.0%
!! CTTCTGCTTTGGAATTGAGC+TGG - chr6_4:78156121-78156140 None:intergenic 45.0%
AAAACTGCTGCTGAGGTCAA+AGG + chr6_4:78156426-78156445 Msa0998000:CDS 45.0%
CAATATCGTGGTCCCAATTG+CGG - chr6_4:78156512-78156531 None:intergenic 45.0%
CAATTGGGACCACGATATTG+CGG + chr6_4:78156512-78156531 Msa0998000:intron 45.0%
GTTTGCAGCGTAACGTTAAC+TGG + chr6_4:78156607-78156626 Msa0998000:intron 45.0%
GACATCACTTAAGTCTCGTG+TGG + chr6_4:78157118-78157137 Msa0998000:intron 45.0%
CTGTGTCACCATTCTAAGTG+TGG + chr6_4:78157743-78157762 Msa0998000:CDS 45.0%
CACCATTCTAAGTGTGGAAG+AGG + chr6_4:78157749-78157768 Msa0998000:CDS 45.0%
CACCTCTTCCACACTTAGAA+TGG - chr6_4:78157754-78157773 None:intergenic 45.0%
AGAGGTGAAGAAGAAAACCG+AGG + chr6_4:78157767-78157786 Msa0998000:CDS 45.0%
TTTGGTGAAGGAGGCTTCTT+AGG - chr6_4:78158059-78158078 None:intergenic 45.0%
TGCACCATTGAGGACATTGT+TGG - chr6_4:78158085-78158104 None:intergenic 45.0%
GGAGCATAACCATAACACCT+TGG - chr6_4:78158113-78158132 None:intergenic 45.0%
TGGAGCAAGGTTCAGGATTT+AGG - chr6_4:78158190-78158209 None:intergenic 45.0%
! CTTACTCACATGATGGAGCA+AGG - chr6_4:78158203-78158222 None:intergenic 45.0%
!! TAAAACAAAAAAAATATTTA+AGG + chr6_4:78156673-78156692 Msa0998000:intron 5.0%
!! AAAACAAAAAAAATATTTAA+GGG + chr6_4:78156674-78156693 Msa0998000:intron 5.0%
!!! TTTTTTACTAATTTTTTTAT+AGG + chr6_4:78158568-78158587 Msa0998000:three_prime_UTR 5.0%
AGCACTCAAAACTGCTGCTG+AGG + chr6_4:78156419-78156438 Msa0998000:CDS 50.0%
! TTATGGGCTGCAACCGCAAT+TGG + chr6_4:78156496-78156515 Msa0998000:intron 50.0%
! TATGGGCTGCAACCGCAATT+GGG + chr6_4:78156497-78156516 Msa0998000:intron 50.0%
! TGCACATGCCCAAGGTGTTA+TGG + chr6_4:78158101-78158120 Msa0998000:CDS 50.0%
! CACATGATGGAGCAAGGTTC+AGG - chr6_4:78158197-78158216 None:intergenic 50.0%
CAAGCCGCATAATTAGGTGG+TGG + chr6_4:78158316-78158335 Msa0998000:three_prime_UTR 50.0%
CTTGGGCATGTGCACCATTG+AGG - chr6_4:78158095-78158114 None:intergenic 55.0%
CTCAATGGTGCACATGCCCA+AGG + chr6_4:78158093-78158112 Msa0998000:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 78155939 78158710 78155939 ID=Msa0998000;Name=Msa0998000
chr6_4 mRNA 78155939 78158710 78155939 ID=Msa0998000-mRNA-1;Parent=Msa0998000;Name=Msa0998000-mRNA-1;_AED=0.16;_eAED=0.16;_QI=287|1|1|1|0.5|0.33|3|493|221
chr6_4 exon 78155939 78156231 78155939 ID=Msa0998000-mRNA-1:exon:14793;Parent=Msa0998000-mRNA-1
chr6_4 exon 78156369 78156447 78156369 ID=Msa0998000-mRNA-1:exon:14794;Parent=Msa0998000-mRNA-1
chr6_4 exon 78157637 78158710 78157637 ID=Msa0998000-mRNA-1:exon:14795;Parent=Msa0998000-mRNA-1
chr6_4 five_prime_UTR 78155939 78156225 78155939 ID=Msa0998000-mRNA-1:five_prime_utr;Parent=Msa0998000-mRNA-1
chr6_4 CDS 78156226 78156231 78156226 ID=Msa0998000-mRNA-1:cds;Parent=Msa0998000-mRNA-1
chr6_4 CDS 78156369 78156447 78156369 ID=Msa0998000-mRNA-1:cds;Parent=Msa0998000-mRNA-1
chr6_4 CDS 78157637 78158217 78157637 ID=Msa0998000-mRNA-1:cds;Parent=Msa0998000-mRNA-1
chr6_4 three_prime_UTR 78158218 78158710 78158218 ID=Msa0998000-mRNA-1:three_prime_utr;Parent=Msa0998000-mRNA-1
Gene Sequence

>Msa0998000

ATGAAGCAAAAGATAGTTATAAAGTTGCAAATGGATTGTGAGAAATGCAGAAACAAAGCACTCAAAACTGCTGCTGAGGTCAAAGGTGTGACTTCAGTATCATTGGAAGGAGATGATAAAGATAAGATATCTGTCACCGGTGATAATGTAGACACAGTTTGCTTAGCCAACATGCTGAAAAAAAAGTTCAACTGTGTCACCATTCTAAGTGTGGAAGAGGTGAAGAAGAAAACCGAGGAAGAAAAAAAGAAAGAAGAAGAAAAGAAAAAAGAAGAAGAAAAGAAAAAGATGATGGAAGCATGTCGTACTGTTTTGTTTGGTTCTTGCAAGTCTTGCAATACTCATTGTAATGGTAAGTGTAAAAAATGCACATGTGGAAGTGCAAAATGTGATGGCATACAATGTGTCACAATTTGCTTCAAGTGTGAAAATCCAAAGAGTTGTTGTGAGTGTAAACCTAATCCAAAGAGTTGTTGTGACAACAAGAAGTGTGATGGTTGCACTCCTAAGAAGCCTCCTTCACCAAAAATCCAACAATGTCCTCAATGGTGCACATGCCCAAGGTGTTATGGTTATGCTCCATATCAACCTTATTGTAACCCTTATCCACCACCTAATTATGTGGTTTGTTATGACCTAAATCCTGAACCTTGCTCCATCATGTGA
Protein sequence

>Msa0998000

MKQKIVIKLQMDCEKCRNKALKTAAEVKGVTSVSLEGDDKDKISVTGDNVDTVCLANMLKKKFNCVTILSVEEVKKKTEEEKKKEEEKKKEEEKKKMMEACRTVLFGSCKSCNTHCNGKCKKCTCGSAKCDGIQCVTICFKCENPKSCCECKPNPKSCCDNKKCDGCTPKKPPSPKIQQCPQWCTCPRCYGYAPYQPYCNPYPPPNYVVCYDLNPEPCSIM*