AlfalfaGEDB | Msa0329320

M. sativa cultivar zhongmu-4 / Msa0329320

KEGG: K02726
GO: GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005839 GO:0005840 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0010035 GO:0010038 GO:0010043 GO:0019773 GO:0022626 GO:0032991 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0098542 GO:1902494 GO:1905368 GO:1905369 GO:1990904
PFAM: PF10584.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0329320	XP_013442471.1	100.000	235	0	0	1	235	1	235	8.13e-171	479

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0329320	sp\|O23708\|PSA2A_ARATH	94.043	235	14	0	1	235	1	235	5.64e-163	452

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003720	Msa0329320	0.811100	8.288887e-51	-8.615850e-47
Msa0018620	Msa0329320	0.819425	1.169451e-52	-8.615850e-47
Msa0026430	Msa0329320	0.807163	5.779718e-50	-8.615850e-47
Msa0026910	Msa0329320	0.840623	7.776678e-58	-8.615850e-47
Msa0027320	Msa0329320	0.800343	1.506631e-48	-8.615850e-47
Msa0027390	Msa0329320	0.817648	2.956719e-52	-8.615850e-47
Msa0034270	Msa0329320	0.802536	5.355625e-49	-8.615850e-47
Msa0040450	Msa0329320	0.826663	2.397740e-54	-8.615850e-47
Msa0045790	Msa0329320	0.843553	1.305676e-58	-8.615850e-47
Msa0046160	Msa0329320	0.841506	4.559320e-58	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0329320	MtrunA17_Chr0c02g0489661	100.000	235	0	0	1	235	1	235	7.48e-175	479
Msa0329320	MtrunA17_Chr8g0371571	38.767	227	134	3	4	228	2	225	8.69e-52	168
Msa0329320	MtrunA17_Chr2g0308541	38.961	231	136	4	6	231	9	239	9.79e-52	168
Msa0329320	MtrunA17_Chr7g0243311	36.752	234	141	4	5	231	4	237	4.81e-49	161
Msa0329320	MtrunA17_Chr8g0360781	38.961	231	136	4	6	231	9	239	4.06e-48	159
Msa0329320	MtrunA17_Chr1g0210811	35.526	228	142	2	4	228	6	231	1.45e-47	157
Msa0329320	MtrunA17_Chr5g0401801	41.270	189	109	2	5	191	4	192	1.04e-46	154
Msa0329320	MtrunA17_Chr2g0283841	37.445	227	137	3	4	228	2	225	1.85e-46	154
Msa0329320	MtrunA17_Chr1g0155371	35.111	225	141	3	1	222	1	223	1.41e-41	143
Msa0329320	MtrunA17_Chr6g0459741	36.612	183	116	0	6	188	8	190	3.43e-39	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0329320	AT1G16470.1	94.043	235	14	0	1	235	1	235	5.73e-164	452
Msa0329320	AT1G16470.2	94.043	235	14	0	1	235	1	235	5.73e-164	452
Msa0329320	AT1G79210.1	93.191	235	16	0	1	235	1	235	1.07e-162	449
Msa0329320	AT1G79210.2	93.191	235	16	0	1	235	1	235	1.07e-162	449
Msa0329320	AT1G79210.3	93.191	235	16	0	1	235	1	235	1.07e-162	449
Msa0329320	AT5G66140.1	39.035	228	133	4	4	228	2	226	3.97e-50	164
Msa0329320	AT3G51260.1	38.596	228	134	4	4	228	2	226	9.19e-49	160
Msa0329320	AT3G51260.2	39.623	212	123	3	4	213	2	210	1.66e-48	160
Msa0329320	AT1G53850.1	35.526	228	142	2	4	228	6	231	1.68e-47	157
Msa0329320	AT1G53850.2	35.526	228	142	2	4	228	6	231	1.68e-47	157

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Find 70 sgRNAs with CRISPR-Local

Find 418 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAATCATCAATTTCAGC+TGG	0.537679	2_4:-42502320	None:intergenic
AACACAACTTCGGGAACACA+AGG	0.594447	2_4:-42502379	None:intergenic
AACATTGAAATCGGCATCAT+TGG	0.369450	2_4:+42501379	Msa0329320:CDS
ACAAGCAGGGACACTCCAAA+TGG	0.570540	2_4:-42500280	None:intergenic
ACAAGTTGAGTGACAGGGAT+TGG	0.466755	2_4:-42499527	None:intergenic
AGAAAGAGAAAGAGGAAGAA+TGG	0.441637	2_4:-42496078	None:intergenic
AGCGTAGATGTATGTAAGTC+AGG	0.402226	2_4:-42496022	None:intergenic
AGCTGAAATTGATGATTATT+TGG	0.158426	2_4:+42502322	Msa0329320:CDS
AGTTTAATTGAATTTCAAGA+TGG	0.499567	2_4:+42502415	Msa0329320:three_prime_UTR
ATCAAACCCAGCAACAAGCA+GGG	0.606489	2_4:-42500293	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAGAATTAGAAAGAGAAAG+AGG	-	chr2_4:42496089-42496108	None:intergenic	30.0%
TCTTTCTCTTTCTAATTCTC+AGG	+	chr2_4:42496088-42496107	Msa0329320:five_prime_UTR	30.0%
AGTAATTATGGGAAAAACGA+AGG	-	chr2_4:42496123-42496142	None:intergenic	30.0%
AATAAGAACCCTAAAACATG+AGG	-	chr2_4:42496417-42496436	None:intergenic	30.0%
TGATACATTACTAGGGTTTA+GGG	+	chr2_4:42496515-42496534	Msa0329320:intron	30.0%
AGAAATGTAATGGCAGAAAT+CGG	-	chr2_4:42496810-42496829	None:intergenic	30.0%
TGATGTAAATATCTTGTCTG+TGG	+	chr2_4:42496948-42496967	Msa0329320:intron	30.0%
ATGCTTCAGTACTATCATAT+TGG	+	chr2_4:42497608-42497627	Msa0329320:intron	30.0%
AATTGGATACAATCTTGAAG+TGG	-	chr2_4:42497962-42497981	None:intergenic	30.0%
CTTCAAGATTGTATCCAATT+TGG	+	chr2_4:42497962-42497981	Msa0329320:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	42496002	42502561	42496002	ID=Msa0329320;Name=Msa0329320
chr2_4	mRNA	42496002	42502561	42496002	ID=Msa0329320-mRNA-1;Parent=Msa0329320;Name=Msa0329320-mRNA-1;_AED=0.14;_eAED=0.14;_QI=146\|1\|1\|1\|0.81\|0.66\|12\|180\|235
chr2_4	exon	42496002	42496109	42496002	ID=Msa0329320-mRNA-1:exon:13125;Parent=Msa0329320-mRNA-1
chr2_4	exon	42496212	42496367	42496212	ID=Msa0329320-mRNA-1:exon:13126;Parent=Msa0329320-mRNA-1
chr2_4	exon	42497827	42497891	42497827	ID=Msa0329320-mRNA-1:exon:13127;Parent=Msa0329320-mRNA-1
chr2_4	exon	42498304	42498350	42498304	ID=Msa0329320-mRNA-1:exon:13128;Parent=Msa0329320-mRNA-1
chr2_4	exon	42499012	42499087	42499012	ID=Msa0329320-mRNA-1:exon:13129;Parent=Msa0329320-mRNA-1
chr2_4	exon	42499524	42499591	42499524	ID=Msa0329320-mRNA-1:exon:13130;Parent=Msa0329320-mRNA-1
chr2_4	exon	42500270	42500339	42500270	ID=Msa0329320-mRNA-1:exon:13131;Parent=Msa0329320-mRNA-1
chr2_4	exon	42500484	42500569	42500484	ID=Msa0329320-mRNA-1:exon:13132;Parent=Msa0329320-mRNA-1
chr2_4	exon	42500767	42500829	42500767	ID=Msa0329320-mRNA-1:exon:13133;Parent=Msa0329320-mRNA-1
chr2_4	exon	42501354	42501419	42501354	ID=Msa0329320-mRNA-1:exon:13134;Parent=Msa0329320-mRNA-1
chr2_4	exon	42502310	42502480	42502310	ID=Msa0329320-mRNA-1:exon:13135;Parent=Msa0329320-mRNA-1
chr2_4	exon	42502504	42502561	42502504	ID=Msa0329320-mRNA-1:exon:13136;Parent=Msa0329320-mRNA-1
chr2_4	five_prime_UTR	42496002	42496109	42496002	ID=Msa0329320-mRNA-1:five_prime_utr;Parent=Msa0329320-mRNA-1
chr2_4	five_prime_UTR	42496212	42496249	42496212	ID=Msa0329320-mRNA-1:five_prime_utr;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42496250	42496367	42496250	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42497827	42497891	42497827	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42498304	42498350	42498304	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42499012	42499087	42499012	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42499524	42499591	42499524	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42500270	42500339	42500270	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42500484	42500569	42500484	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42500767	42500829	42500767	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42501354	42501419	42501354	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	CDS	42502310	42502358	42502310	ID=Msa0329320-mRNA-1:cds;Parent=Msa0329320-mRNA-1
chr2_4	three_prime_UTR	42502359	42502480	42502359	ID=Msa0329320-mRNA-1:three_prime_utr;Parent=Msa0329320-mRNA-1
chr2_4	three_prime_UTR	42502504	42502561	42502504	ID=Msa0329320-mRNA-1:three_prime_utr;Parent=Msa0329320-mRNA-1

Gene Sequence

>Msa0329320

ATGGGAGATAGTCAATACTCGTTTTCTCTTACAACTTTTAGTCCTTCTGGTAAGCTTGTTCAGATTGAACATGCTTTAACTGCTGTTGGTTCTGGACAAACTTCTCTCGGTATCAAAGCTGCAAATGGAGTTGTCATTGCTACTGAGAAGAAACTGCCATCTATATTGGTTGATGAAGCATCAGTCCAAAAAATTCAGTTATTAACACCAAATATAGGTGTTGTATACAGTGGCATGGGTCCTGATTTCCGAGTTTTGGTTCGAAAAAGCAGGAAACAGGCTGAGCAGTATCACCGATTGTATAAGGAACCAATCCCTGTCACTCAACTTGTGAGGGAAGTTGCTGCTGTCATGCAGGAATTTACTCAGTCTGGTGGTGTTAGGCCATTTGGAGTGTCCCTGCTTGTTGCTGGGTTTGATGATAATGGACCACAACTATATCAGGTGGATCCATCAGGTTCATATTTTTCTTGGAAAGCTTCTGCTATGGGGAAAAATGTTTCTAATGCTAAGACATTTCTTGAGAAGAGATACACTGATGATATGGAGCTTGATGATGCAGTCCACACAGCCATATTGACACTGAAGGAAGGGTTTGAGGGACAGATCTCAGCAAAAAACATTGAAATCGGCATCATTGGTGCAGACAAAAAGTTCAGGGTATTGACACCAGCTGAAATTGATGATTATTTGGGTGAAGTAGAATAA

Protein sequence

>Msa0329320

MGDSQYSFSLTTFSPSGKLVQIEHALTAVGSGQTSLGIKAANGVVIATEKKLPSILVDEASVQKIQLLTPNIGVVYSGMGPDFRVLVRKSRKQAEQYHRLYKEPIPVTQLVREVAAVMQEFTQSGGVRPFGVSLLVAGFDDNGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTDDMELDDAVHTAILTLKEGFEGQISAKNIEIGIIGADKKFRVLTPAEIDDYLGEVE*