AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0689010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689010 MtrunA17_Chr4g0030551 97.985 546 11 0 1 546 1 546 0.0 1092
Msa0689010 MtrunA17_Chr2g0327711 78.479 539 111 3 12 546 7 544 0.0 832
Msa0689010 MtrunA17_Chr7g0263421 60.000 525 205 3 22 546 1 520 0.0 612
Msa0689010 MtrunA17_Chr7g0276681 56.981 530 215 7 17 546 30 546 0.0 563
Msa0689010 MtrunA17_Chr1g0212971 46.570 554 255 9 16 546 58 593 1.84e-154 454
Msa0689010 MtrunA17_Chr1g0161231 43.561 528 272 8 35 546 14 531 9.46e-145 427
Msa0689010 MtrunA17_Chr8g0391711 45.010 491 249 7 66 546 59 538 7.22e-138 410
Msa0689010 MtrunA17_Chr7g0263471 43.613 548 268 9 19 546 56 582 5.05e-129 389
Msa0689010 MtrunA17_Chr2g0331191 25.847 561 321 13 28 514 67 606 6.72e-40 153
Msa0689010 MtrunA17_Chr2g0331191 25.781 256 183 4 32 286 2 251 1.44e-17 86.3
Msa0689010 MtrunA17_Chr2g0331201 25.813 523 290 14 32 478 71 571 8.49e-35 139
Msa0689010 MtrunA17_Chr2g0331201 23.016 252 148 4 72 288 38 278 2.14e-18 89.0
Msa0689010 MtrunA17_Chr5g0439161 26.566 463 284 16 67 491 207 651 1.48e-19 92.4
Msa0689010 MtrunA17_Chr7g0233331 24.297 391 232 11 74 432 9 367 1.34e-17 85.1
Msa0689010 MtrunA17_Chr3g0122941 26.761 284 177 10 35 294 109 385 1.75e-16 82.8
Msa0689010 MtrunA17_Chr3g0122941 33.500 200 121 5 58 248 94 290 8.09e-16 80.9
Msa0689010 MtrunA17_Chr3g0122941 28.571 196 122 4 100 282 100 290 2.17e-13 73.2
Msa0689010 MtrunA17_Chr6g0458951 33.171 205 116 6 108 294 192 393 3.20e-16 82.0
Msa0689010 MtrunA17_Chr8g0369921 23.148 324 212 6 74 384 10 309 7.89e-16 80.1
Msa0689010 MtrunA17_Chr8g0370361 25.000 288 180 6 109 384 47 310 1.22e-15 79.3
Msa0689010 MtrunA17_Chr5g0439361 22.955 379 256 8 123 480 5 368 1.36e-15 79.0
Msa0689010 MtrunA17_Chr1g0211241 30.000 190 130 3 109 298 139 325 1.62e-15 79.7
Msa0689010 MtrunA17_Chr8g0367671 24.315 292 183 5 109 386 48 315 4.01e-15 77.8
Msa0689010 MtrunA17_Chr8g0376971 31.013 158 92 2 108 248 188 345 7.74e-15 77.4
Msa0689010 MtrunA17_Chr8g0376971 30.769 195 119 3 70 248 102 296 1.02e-14 77.4
Msa0689010 MtrunA17_Chr3g0136651 22.902 441 236 14 107 477 3 409 1.97e-14 75.5
Msa0689010 MtrunA17_Chr5g0418551 33.750 160 101 4 143 300 58 214 2.41e-14 73.2
Msa0689010 MtrunA17_Chr8g0364031 25.397 252 172 3 77 315 13 261 2.68e-14 75.1
Msa0689010 MtrunA17_Chr1g0178581 25.688 327 171 9 106 384 9 311 1.10e-13 73.2
Msa0689010 MtrunA17_Chr8g0361991 28.387 155 109 1 109 261 11 165 1.42e-13 73.2
Msa0689010 MtrunA17_Chr7g0262731 27.200 250 164 8 103 341 7 249 3.55e-13 72.4
Msa0689010 MtrunA17_Chr2g0328031 28.095 210 126 4 71 261 12 215 4.70e-13 71.2
Msa0689010 MtrunA17_Chr3g0083331 22.785 316 209 7 104 384 5 320 5.51e-13 71.2
Msa0689010 MtrunA17_Chr8g0353311 23.939 330 174 8 107 384 12 316 8.29e-13 70.5
Msa0689010 MtrunA17_Chr5g0439351 20.089 448 306 9 62 480 261 685 1.01e-12 70.9
Msa0689010 MtrunA17_Chr5g0409271 31.390 223 126 7 101 322 8 204 1.48e-12 68.2
Msa0689010 MtrunA17_Chr5g0439191 33.766 154 93 5 135 280 1 153 3.29e-12 65.5
Msa0689010 MtrunA17_Chr6g0461061 28.472 288 169 13 34 315 246 502 3.45e-12 68.9
Msa0689010 MtrunA17_Chr6g0461061 30.811 185 105 7 29 213 308 469 6.57e-11 65.1
Msa0689010 MtrunA17_Chr8g0354071 22.741 343 215 8 116 432 2 320 4.32e-12 67.8
Msa0689010 MtrunA17_Chr2g0293861 32.680 153 94 5 136 280 27 178 4.44e-12 65.5
Msa0689010 MtrunA17_Chr8g0366431 24.113 282 165 7 173 430 32 288 6.53e-12 67.4
Msa0689010 MtrunA17_Chr1g0196261 29.018 224 131 10 99 298 210 429 8.57e-12 67.4
Msa0689010 MtrunA17_Chr4g0064931 31.606 193 123 6 109 298 40 226 9.17e-12 67.8
Msa0689010 MtrunA17_Chr4g0031491 23.167 341 223 9 68 382 3 330 1.11e-11 67.0
Msa0689010 MtrunA17_Chr4g0063141 30.357 168 112 4 136 303 548 710 1.25e-11 67.8
Msa0689010 MtrunA17_Chr3g0093531 22.741 343 215 8 116 432 2 320 1.55e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689010 AT1G07710.2 69.636 550 156 5 1 546 1 543 0.0 724
Msa0689010 AT1G07710.1 69.636 550 156 5 1 546 1 543 0.0 724
Msa0689010 AT5G60070.1 64.954 545 182 5 7 546 8 548 0.0 690
Msa0689010 AT5G02620.4 61.278 532 190 4 19 546 5 524 0.0 637
Msa0689010 AT5G02620.1 61.278 532 190 4 19 546 5 524 0.0 637
Msa0689010 AT5G02620.3 61.278 532 190 4 19 546 5 524 0.0 637
Msa0689010 AT5G02620.5 59.140 558 183 7 19 546 5 547 0.0 627
Msa0689010 AT5G02620.2 59.140 558 183 7 19 546 5 547 0.0 627
Msa0689010 AT2G31820.1 55.801 543 226 8 8 546 130 662 0.0 566
Msa0689010 AT1G05640.1 56.772 539 217 9 15 546 98 627 0.0 553
Msa0689010 AT3G12360.2 46.069 547 264 9 16 546 47 578 2.31e-147 436
Msa0689010 AT3G12360.1 46.069 547 264 9 16 546 59 590 2.83e-147 436
Msa0689010 AT3G09550.1 44.161 548 270 8 19 546 76 607 8.83e-137 410
Msa0689010 AT3G09550.3 44.161 548 270 8 19 546 76 607 8.83e-137 410
Msa0689010 AT3G09550.2 44.161 548 270 8 19 546 83 614 1.51e-136 409
Msa0689010 AT2G01680.1 43.943 487 250 4 35 507 14 491 2.70e-134 401
Msa0689010 AT2G24600.3 25.749 501 315 17 32 501 72 546 3.32e-38 149
Msa0689010 AT2G24600.4 25.749 501 315 17 32 501 72 546 3.32e-38 149
Msa0689010 AT1G34050.1 26.854 499 318 12 21 507 75 538 6.14e-38 147
Msa0689010 AT5G54710.1 25.775 516 323 13 32 523 103 582 5.06e-35 139
Msa0689010 AT5G54710.2 25.775 516 323 13 32 523 148 627 6.59e-35 139
Msa0689010 AT5G54710.2 27.155 232 156 3 94 323 5 225 8.09e-13 71.6
Msa0689010 AT5G54710.2 23.958 288 177 5 32 290 19 293 1.22e-12 70.9
Msa0689010 AT1G10340.1 23.985 517 297 13 72 501 39 546 4.26e-31 127
Msa0689010 AT1G10340.2 23.699 519 335 13 31 501 37 542 6.36e-31 127
Msa0689010 AT4G03500.1 24.412 553 331 15 4 486 64 599 1.49e-29 123
Msa0689010 AT4G03500.2 24.774 553 329 16 4 486 41 576 1.80e-29 123
Msa0689010 AT4G03500.3 24.774 553 329 16 4 486 41 576 1.80e-29 123
Msa0689010 AT4G14390.2 26.107 429 267 10 33 428 149 560 1.12e-26 115
Msa0689010 AT4G14390.1 26.279 430 266 10 33 428 168 580 1.30e-26 114
Msa0689010 AT4G14390.4 26.107 429 267 10 33 428 168 579 1.32e-26 114
Msa0689010 AT4G03470.1 23.695 498 321 14 27 491 149 620 4.55e-25 110
Msa0689010 AT1G03670.1 22.182 559 331 15 4 472 33 577 1.11e-24 108
Msa0689010 AT4G03460.1 24.508 457 273 14 32 429 81 524 1.93e-24 107
Msa0689010 AT4G03460.1 33.824 136 79 2 134 258 75 210 5.80e-13 72.0
Msa0689010 AT4G03480.1 26.234 385 222 9 67 429 312 656 9.41e-24 105
Msa0689010 AT4G03460.2 23.888 427 258 12 62 429 74 492 9.82e-24 105
Msa0689010 AT4G03480.2 26.234 385 222 9 67 429 309 653 1.02e-23 105
Msa0689010 AT5G50140.1 24.038 520 306 17 27 517 57 516 1.72e-23 104
Msa0689010 AT5G50140.1 24.815 270 179 6 80 331 6 269 1.28e-14 77.0
Msa0689010 AT2G24600.2 26.463 393 241 14 32 399 72 441 8.34e-23 102
Msa0689010 AT2G24600.1 26.463 393 241 14 32 399 72 441 8.34e-23 102
Msa0689010 AT5G54700.2 26.409 337 176 9 165 486 23 302 2.74e-22 98.6
Msa0689010 AT4G05040.3 25.293 427 281 10 30 429 148 563 3.37e-22 100
Msa0689010 AT4G05040.3 28.161 174 110 3 140 298 82 255 7.30e-11 65.1
Msa0689010 AT4G05040.2 25.293 427 281 10 30 429 148 563 3.37e-22 100
Msa0689010 AT4G05040.2 28.161 174 110 3 140 298 82 255 7.30e-11 65.1
Msa0689010 AT4G05040.5 25.293 427 281 10 30 429 148 563 3.37e-22 100
Msa0689010 AT4G05040.5 28.161 174 110 3 140 298 82 255 7.30e-11 65.1
Msa0689010 AT4G05040.4 25.293 427 281 10 30 429 148 563 3.37e-22 100
Msa0689010 AT4G05040.4 28.161 174 110 3 140 298 82 255 7.30e-11 65.1
Msa0689010 AT4G05040.1 25.293 427 281 10 30 429 148 563 3.37e-22 100
Msa0689010 AT4G05040.1 28.161 174 110 3 140 298 82 255 7.30e-11 65.1
Msa0689010 AT4G05040.6 25.293 427 281 10 30 429 148 563 3.37e-22 100
Msa0689010 AT4G05040.6 28.161 174 110 3 140 298 82 255 7.30e-11 65.1
Msa0689010 AT4G05040.7 25.293 427 281 10 30 429 148 563 3.37e-22 100
Msa0689010 AT4G05040.7 28.161 174 110 3 140 298 82 255 7.30e-11 65.1
Msa0689010 AT4G10720.2 26.488 336 203 9 109 427 41 349 4.94e-22 99.0
Msa0689010 AT4G10720.1 27.645 293 174 7 109 384 41 312 8.63e-22 98.6
Msa0689010 AT4G14390.3 25.521 384 242 9 33 383 149 521 4.22e-19 90.9
Msa0689010 AT5G54700.1 24.927 341 197 9 170 486 34 339 5.98e-19 90.1
Msa0689010 AT5G51160.1 25.135 370 219 11 135 461 43 397 8.90e-19 89.4
Msa0689010 AT4G14400.2 23.841 453 255 10 33 429 71 489 1.70e-18 89.4
Msa0689010 AT4G14400.3 23.841 453 255 10 33 429 71 489 1.70e-18 89.4
Msa0689010 AT4G14400.1 23.841 453 255 10 33 429 137 555 1.83e-18 89.4
Msa0689010 AT4G03490.2 21.116 502 286 14 62 500 64 518 2.29e-18 89.4
Msa0689010 AT4G03490.3 21.553 515 306 14 62 500 84 576 2.70e-18 89.0
Msa0689010 AT4G03490.1 21.116 502 286 14 62 500 74 528 3.53e-18 88.6
Msa0689010 AT4G03490.4 21.553 515 306 14 62 500 84 576 3.70e-18 88.6
Msa0689010 AT4G03490.5 21.553 515 306 14 62 500 74 566 3.76e-18 88.6
Msa0689010 AT4G03440.2 25.464 377 238 13 67 429 262 609 2.59e-17 85.9
Msa0689010 AT4G03440.3 25.464 377 238 13 67 429 262 609 2.59e-17 85.9
Msa0689010 AT4G03440.1 25.464 377 238 13 67 429 269 616 2.73e-17 85.9
Msa0689010 AT4G03450.2 24.851 503 268 17 27 468 101 554 7.38e-17 84.3
Msa0689010 AT4G03450.2 24.176 273 184 6 35 290 40 306 2.52e-11 66.6
Msa0689010 AT5G15500.2 25.526 333 176 7 108 388 5 317 8.02e-17 83.6
Msa0689010 AT4G03450.1 24.851 503 268 17 27 468 101 554 1.20e-16 83.6
Msa0689010 AT4G03450.1 24.176 273 184 6 35 290 40 306 3.18e-11 66.6
Msa0689010 AT2G03430.1 32.353 204 131 6 56 257 33 231 1.21e-15 77.0
Msa0689010 AT2G03430.1 34.759 187 109 7 64 245 8 186 3.43e-13 70.1
Msa0689010 AT2G03430.1 31.818 198 119 9 109 298 16 205 2.35e-11 64.3
Msa0689010 AT3G04140.1 26.480 321 193 11 35 316 106 422 3.72e-15 79.0
Msa0689010 AT3G04140.1 30.542 203 120 4 100 281 97 299 9.61e-14 74.7
Msa0689010 AT3G01750.2 27.823 248 138 7 35 248 109 349 6.58e-15 78.2
Msa0689010 AT3G01750.1 27.823 248 138 7 35 248 109 349 6.58e-15 78.2
Msa0689010 AT5G20350.1 30.542 203 132 6 99 298 25 221 9.61e-13 71.2
Msa0689010 AT5G57740.1 28.378 222 120 6 109 294 55 273 1.75e-12 70.1
Msa0689010 AT1G14500.1 24.255 235 159 4 109 326 41 273 2.11e-12 69.7
Msa0689010 AT1G14500.1 27.922 154 107 3 112 262 8 160 1.65e-11 67.0
Msa0689010 AT5G54620.1 28.182 220 142 5 109 313 41 259 2.31e-12 69.7
Msa0689010 AT5G54620.1 28.188 149 105 1 116 262 12 160 1.58e-11 67.0
Msa0689010 AT4G19150.1 35.833 120 75 2 109 228 20 137 2.32e-12 67.4
Msa0689010 AT2G28840.1 29.341 167 103 4 109 261 50 215 5.32e-12 68.6
Msa0689010 AT2G26650.1 34.395 157 97 5 104 260 550 700 7.75e-12 68.6
Msa0689010 AT2G26650.2 34.395 157 97 5 104 260 445 595 8.95e-12 68.6
Msa0689010 AT3G04710.3 28.571 287 185 9 10 294 227 495 2.43e-11 67.0
Msa0689010 AT2G14255.1 30.201 149 101 3 109 256 95 241 3.12e-11 66.2
Msa0689010 AT3G04710.1 28.571 287 185 8 10 294 3 271 3.48e-11 65.9
Msa0689010 AT3G04710.2 28.750 320 165 14 10 321 3 267 4.56e-11 65.5
Msa0689010 AT2G25600.1 29.268 205 135 7 112 314 552 748 5.95e-11 65.9

Find 124 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTGGGGTAACTATTAT+TGG 0.156648 4_4:+55696002 Msa0689010:CDS
AATAAACATGGTTGATAATA+AGG 0.174079 4_4:+55694473 Msa0689010:CDS
CATTGGGTACTATGAGTTAT+TGG 0.186716 4_4:+55696045 Msa0689010:CDS
TTCTTCCTTTGAGCCCTTAC+TGG 0.196107 4_4:-55693258 None:intergenic
CTATGAGTTATTGGGTTATT+AGG 0.231803 4_4:+55696054 Msa0689010:CDS
TCAACCATGTTTATTGTTGA+TGG 0.242519 4_4:-55694465 None:intergenic
TACCTGAGTTCTTCCCTTCC+TGG 0.243726 4_4:-55694520 None:intergenic
TTTCCTGGAGGTATGTGTTT+TGG 0.248926 4_4:-55695732 None:intergenic
GCTGCTAAACAAGGGGATTT+AGG 0.260704 4_4:+55693552 Msa0689010:CDS
ACAAAAGGAGTTCATTAGTT+TGG 0.269564 4_4:+55693183 Msa0689010:five_prime_UTR
ACCCAATGTTGTAGCCATTA+TGG 0.293380 4_4:-55696030 None:intergenic
TGTAACGCGGACTGATAAAA+AGG 0.298877 4_4:+55694371 Msa0689010:CDS
CTTTATGTTGCTGCTGAGTA+TGG 0.312082 4_4:+55693438 Msa0689010:CDS
AGATATTGTAAAGATCTTAA+TGG 0.322743 4_4:+55694128 Msa0689010:intron
CAATTGGGGTAACTATTATT+GGG 0.329062 4_4:+55696003 Msa0689010:CDS
ATGGTTATGTTGATATGGTT+AGG 0.342881 4_4:+55693457 Msa0689010:CDS
TTGCATTCTGCTGCAAGAAA+TGG 0.353298 4_4:+55694303 Msa0689010:CDS
AAACAAAGGTGGCTTGCAAT+TGG 0.357257 4_4:+55695987 Msa0689010:CDS
AACAAAGGTGGCTTGCAATT+GGG 0.360089 4_4:+55695988 Msa0689010:CDS
ATTGGGTACTATGAGTTATT+GGG 0.363533 4_4:+55696046 Msa0689010:CDS
CAGATTATCAAGTTGATACT+TGG 0.370218 4_4:+55695327 Msa0689010:intron
TGAGTATGGTTATGTTGATA+TGG 0.383585 4_4:+55693452 Msa0689010:CDS
TGGGAACATGGCTTCTTTGA+AGG 0.392889 4_4:+55693341 Msa0689010:CDS
CAAACACGAAGAAGCGTTCA+AGG 0.397506 4_4:+55695573 Msa0689010:CDS
GTTTGGAGAATTGTATTCAA+TGG 0.398213 4_4:+55693200 Msa0689010:exon
TGCTGCAAGAAATGGACATT+TGG 0.400973 4_4:+55694311 Msa0689010:CDS
TATTATTGGGACTACCATAA+TGG 0.409865 4_4:+55696016 Msa0689010:CDS
ACCATAATGGCTACAACATT+GGG 0.411626 4_4:+55696029 Msa0689010:CDS
TTTCATCTCCCTCGCGGTTG+TGG 0.414269 4_4:+55695827 Msa0689010:CDS
TACCATAATGGCTACAACAT+TGG 0.414943 4_4:+55696028 Msa0689010:CDS
GGATCGTCCACGAATTGGCC+TGG 0.416257 4_4:-55695711 None:intergenic
TCTTTGAAGGATACAGTTGA+TGG 0.424259 4_4:+55693354 Msa0689010:CDS
GCTGGAATTAAAGCTAGAAA+TGG 0.430155 4_4:+55693510 Msa0689010:CDS
TTACATTCGGCAGCAAGAGC+TGG 0.434837 4_4:+55693321 Msa0689010:CDS
AGCCTCAGGCTGCACCAGTA+AGG 0.434924 4_4:+55693244 Msa0689010:CDS
TATTATGATCTTGCTGATGC+TGG 0.442983 4_4:+55693492 Msa0689010:CDS
ATAAACATGGTTGATAATAA+GGG 0.448264 4_4:+55694474 Msa0689010:CDS
GCAGCCATCTTCACCGTCCC+AGG 0.449913 4_4:+55695693 Msa0689010:CDS
TTTAGTGGAACACACAAGTA+AGG 0.450272 4_4:+55692787 Msa0689010:five_prime_UTR
CGATGATACGCCGTTACATT+CGG 0.459070 4_4:+55693308 Msa0689010:CDS
TGGTTATGTTGATATGGTTA+GGG 0.459988 4_4:+55693458 Msa0689010:CDS
GGCGCTTCACATGGCTGTGA+AGG 0.462145 4_4:+55694401 Msa0689010:CDS
TTAGACACAGCGGAGAAAAC+AGG 0.464410 4_4:+55695399 Msa0689010:CDS
AGCTGAAACTGTGCAGCCTC+AGG 0.466637 4_4:+55693230 Msa0689010:CDS
AGTATTCATCGTGTTTGATT+CGG 0.466809 4_4:+55695797 Msa0689010:CDS
ATTGAAGTCATAAGAACAAA+AGG 0.469472 4_4:+55693168 Msa0689010:five_prime_UTR
GATATACATCATGCACCAAC+AGG 0.476088 4_4:-55692426 None:intergenic
ACAAAGGTGGCTTGCAATTG+GGG 0.480789 4_4:+55695989 Msa0689010:CDS
GCGATTCTTGAGAAGGAGCC+AGG 0.483357 4_4:+55694345 Msa0689010:CDS
GAAAATCTTCAATGGGAAGT+AGG 0.483651 4_4:+55696102 Msa0689010:CDS
GCCTCAGGCTGCACCAGTAA+GGG 0.485466 4_4:+55693245 Msa0689010:CDS
GAGTATAAGAAAATCTTCAA+TGG 0.486162 4_4:+55696094 Msa0689010:CDS
GCAACAATTGCTAGAAGTAA+TGG 0.488412 4_4:+55694270 Msa0689010:CDS
GATCGTCCACGAATTGGCCT+GGG 0.491991 4_4:-55695710 None:intergenic
AGTATAAGAAAATCTTCAAT+GGG 0.497524 4_4:+55696095 Msa0689010:CDS
AGGGAACTAATATATGATGT+TGG 0.505022 4_4:-55692736 None:intergenic
AAGCAAGTGTTGTGAAGTAG+TGG 0.505499 4_4:-55692316 None:intergenic
TTAGGCATAGAATCAATTTG+AGG 0.507230 4_4:+55696072 Msa0689010:CDS
CTTCATATTGCTGCTAAACA+AGG 0.509684 4_4:+55693543 Msa0689010:CDS
TACATTCGGCAGCAAGAGCT+GGG 0.514491 4_4:+55693322 Msa0689010:CDS
GTGAAATATCTATTAGAAGC+AGG 0.514806 4_4:+55694237 Msa0689010:CDS
CATCTCCCTCGCGGTTGTGG+TGG 0.520207 4_4:+55695830 Msa0689010:CDS
CACCGTCCCAGGCCAATTCG+TGG 0.520703 4_4:+55695704 Msa0689010:CDS
AAAATGACGAAGCAATTGAC+GGG 0.522290 4_4:+55693279 Msa0689010:CDS
TTGGCCTGGGACGGTGAAGA+TGG 0.531539 4_4:-55695697 None:intergenic
CGAAAGCAACCGTTGCTATG+AGG 0.535432 4_4:-55695671 None:intergenic
CTTGGACAAAGTGAAACAAA+TGG 0.535956 4_4:+55695345 Msa0689010:CDS
GCGCTTCACATGGCTGTGAA+GGG 0.537881 4_4:+55694402 Msa0689010:CDS
TGCAAGAAATGGACATTTGG+AGG 0.543958 4_4:+55694314 Msa0689010:CDS
CAAAGCAAAGAAGCAAATGA+TGG 0.544079 4_4:+55695881 Msa0689010:CDS
TTTGACGAGTGTGTTCTAGT+TGG 0.546084 4_4:-55695554 None:intergenic
ATGCACCAACAGGAAACAAA+AGG 0.546435 4_4:-55692416 None:intergenic
TCAGTCCGCGTTACAACACC+TGG 0.546749 4_4:-55694363 None:intergenic
GCCCTTACTGGTGCAGCCTG+AGG 0.548134 4_4:-55693246 None:intergenic
TACAGTTGATGGTGCAGAAG+AGG 0.548363 4_4:+55693365 Msa0689010:CDS
TTGTGTTGCAGCAGTGTGCA+AGG 0.551289 4_4:-55694199 None:intergenic
GGCAGCAAGAGCTGGGAACA+TGG 0.556799 4_4:+55693329 Msa0689010:CDS
CGCTTCACATGGCTGTGAAG+GGG 0.560548 4_4:+55694403 Msa0689010:CDS
TGTGAAAGCGATTCTTGAGA+AGG 0.565310 4_4:+55694338 Msa0689010:CDS
GCAGAGTCTTGTGGTAGTAG+AGG 0.567525 4_4:+55694425 Msa0689010:CDS
GAAAATGACGAAGCAATTGA+CGG 0.567757 4_4:+55693278 Msa0689010:CDS
ATTAGAAGCAGGTAGTAGCT+TGG 0.570550 4_4:+55694248 Msa0689010:CDS
CTAATATATGATGTTGGCGT+AGG 0.572037 4_4:-55692730 None:intergenic
CTGCACCAGTAAGGGCTCAA+AGG 0.574302 4_4:+55693253 Msa0689010:CDS
GAGGACCGCGACGACTGTTG+TGG 0.574532 4_4:-55695652 None:intergenic
TTCATATTGCTGCTAAACAA+GGG 0.576566 4_4:+55693544 Msa0689010:CDS
ACAGTTGATGGTGCAGAAGA+GGG 0.580772 4_4:+55693366 Msa0689010:CDS
TGCAAGGCTGTTGTGTTGGA+CGG 0.580949 4_4:-55694183 None:intergenic
ATATTTGCAAAGCAGAATCA+AGG 0.582207 4_4:+55693402 Msa0689010:CDS
AACCAGGAAGGGAAGAACTC+AGG 0.589165 4_4:+55694518 Msa0689010:CDS
CTCAACAAAATGCACACAGA+AGG 0.589714 4_4:+55695609 Msa0689010:CDS
TTGCACACTGCTGCAACACA+AGG 0.589896 4_4:+55694201 Msa0689010:CDS
GGCATGGCAGTAAACAAAAG+TGG 0.596158 4_4:+55695366 Msa0689010:CDS
GCTCTTGCTGCCGAATGTAA+CGG 0.602344 4_4:-55693318 None:intergenic
GGGACAGACGGCGCTTCACA+TGG 0.602708 4_4:+55694392 Msa0689010:CDS
CTTCAATGGGAAGTAGGTCA+AGG 0.604224 4_4:+55696108 Msa0689010:CDS
ATACCTCCAGGAAAATCCCT+TGG 0.605699 4_4:+55695741 Msa0689010:CDS
GAGTGTGTTCTAGTTGGTGA+TGG 0.607313 4_4:-55695548 None:intergenic
GATCCAAAACACATACCTCC+AGG 0.610901 4_4:+55695729 Msa0689010:CDS
AATTGCACAATGGAAAAGAG+TGG 0.611330 4_4:-55696240 None:intergenic
TCATATTGCTGCTAAACAAG+GGG 0.612141 4_4:+55693545 Msa0689010:CDS
TTGCATATAGCAACCAGGAA+GGG 0.612446 4_4:+55694507 Msa0689010:CDS
CAGCGTTGCATATAGCAACC+AGG 0.616173 4_4:+55694502 Msa0689010:CDS
TGTGAAGGGGCAGAGTCTTG+TGG 0.617503 4_4:+55694416 Msa0689010:CDS
TTCTGAATTGTCAATGACTG+TGG 0.617789 4_4:+55694158 Msa0689010:CDS
CACGATGAATACTAGAAACG+CGG 0.624298 4_4:-55695787 None:intergenic
GTTGCATATAGCAACCAGGA+AGG 0.628632 4_4:+55694506 Msa0689010:CDS
ACAAAGTGAAACAAATGGCA+TGG 0.631554 4_4:+55695350 Msa0689010:CDS
TATGTGAATAAATTGCACAA+TGG 0.635969 4_4:-55696250 None:intergenic
GTCCACGAATTGGCCTGGGA+CGG 0.639953 4_4:-55695706 None:intergenic
AGTGTGCAAGGCTGTTGTGT+TGG 0.641164 4_4:-55694187 None:intergenic
CTATTATTAACAAACTAATG+TGG 0.644964 4_4:+55695904 Msa0689010:CDS
AAAAGCATTCTTACAGAACA+CGG 0.650500 4_4:+55695435 Msa0689010:CDS
CGTCGCGGTCCTCATAGCAA+CGG 0.655824 4_4:+55695662 Msa0689010:CDS
AGATCCATCAACAATAAACA+TGG 0.658313 4_4:+55694461 Msa0689010:CDS
AGGAGCCAGGTGTTGTAACG+CGG 0.665895 4_4:+55694358 Msa0689010:CDS
TTGAACCACCACAACCGCGA+GGG 0.673202 4_4:-55695835 None:intergenic
ACGATGAATACTAGAAACGC+GGG 0.673783 4_4:-55695786 None:intergenic
TTTGCAAAGCAGAATCAAGG+TGG 0.681219 4_4:+55693405 Msa0689010:CDS
AAACTCCACAACAGTCGTCG+CGG 0.698686 4_4:+55695647 Msa0689010:CDS
TATAGTACAATTATACAACA+TGG 0.703510 4_4:-55692460 None:intergenic
TTTGAACCACCACAACCGCG+AGG 0.726688 4_4:-55695836 None:intergenic
AAATGACGAAGCAATTGACG+GGG 0.731232 4_4:+55693280 Msa0689010:CDS
CGAAACAGCATTAGACACAG+CGG 0.776505 4_4:+55695389 Msa0689010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAATAAAATAAAAAA+TGG - chr4_4:55692937-55692956 None:intergenic 0.0%
!!! TCTAAATATTTTTACTATAT+TGG + chr4_4:55692615-55692634 Msa0689010:intron 10.0%
!! AGTAAAAATATTTAGAAGAA+AGG - chr4_4:55692611-55692630 None:intergenic 15.0%
!! AAGAAAAAAAAAATCAAAGA+TGG - chr4_4:55692885-55692904 None:intergenic 15.0%
!!! AATCTTGATATTTTAAATTG+CGG + chr4_4:55693647-55693666 Msa0689010:intron 15.0%
!! TTAATTCAATCCTAAAAAAT+TGG + chr4_4:55694869-55694888 Msa0689010:intron 15.0%
!! TATAGTACAATTATACAACA+TGG - chr4_4:55692463-55692482 None:intergenic 20.0%
!!! TAAAAAAGCATTGAGATTTA+AGG - chr4_4:55692771-55692790 None:intergenic 20.0%
!! TCAGTGAAAAAATAAATAAG+AGG - chr4_4:55692830-55692849 None:intergenic 20.0%
!! TTATTATGATGAATGCTTAA+AGG + chr4_4:55692851-55692870 Msa0689010:intron 20.0%
!!! TTTTGTGTTTTTGTTTTTGT+TGG + chr4_4:55693109-55693128 Msa0689010:intron 20.0%
!!! CATTTCTATTATTCAAGTTT+AGG - chr4_4:55693989-55694008 None:intergenic 20.0%
!!! TATCTATTTTATTTTGAACC+AGG + chr4_4:55694078-55694097 Msa0689010:intron 20.0%
!!! ATCTATTTTATTTTGAACCA+GGG + chr4_4:55694079-55694098 Msa0689010:intron 20.0%
!! AGATATTGTAAAGATCTTAA+TGG + chr4_4:55694128-55694147 Msa0689010:intron 20.0%
!!! AATAAACATGGTTGATAATA+AGG + chr4_4:55694473-55694492 Msa0689010:CDS 20.0%
!!! ATAAACATGGTTGATAATAA+GGG + chr4_4:55694474-55694493 Msa0689010:CDS 20.0%
!!! ACGTTATAAACCAATTTTTT+AGG - chr4_4:55694882-55694901 None:intergenic 20.0%
!! ATACAATTCAATTATGCATA+AGG - chr4_4:55695232-55695251 None:intergenic 20.0%
!! CTATTATTAACAAACTAATG+TGG + chr4_4:55695904-55695923 Msa0689010:CDS 20.0%
!! AGTATAAGAAAATCTTCAAT+GGG + chr4_4:55696095-55696114 Msa0689010:CDS 20.0%
! AAGAAGAAAAAGAACAAAAG+AGG - chr4_4:55692580-55692599 None:intergenic 25.0%
!!! AAGGTCTTTTTTTTTGTTAC+AGG + chr4_4:55692703-55692722 Msa0689010:intron 25.0%
!!! AGGTCTTTTTTTTTGTTACA+GGG + chr4_4:55692704-55692723 Msa0689010:intron 25.0%
!! AGATTTAAGGATAGCTTTTA+GGG - chr4_4:55692758-55692777 None:intergenic 25.0%
!!! AAATCTCAATGCTTTTTTAG+TGG + chr4_4:55692772-55692791 Msa0689010:five_prime_UTR 25.0%
!! TTTTATCAGAGCAATGATTT+TGG + chr4_4:55692951-55692970 Msa0689010:intron 25.0%
!!! ATCTTCTTTGATTTGTTTCA+AGG + chr4_4:55693048-55693067 Msa0689010:intron 25.0%
! ATTGAAGTCATAAGAACAAA+AGG + chr4_4:55693168-55693187 Msa0689010:five_prime_UTR 25.0%
! AAAATTCTATGACTGCAATT+TGG - chr4_4:55693779-55693798 None:intergenic 25.0%
!!! TTTTAAAAGAAAACCTTGAG+TGG + chr4_4:55693797-55693816 Msa0689010:intron 25.0%
! CAACTTGTGTTTATTTACTA+CGG - chr4_4:55693955-55693974 None:intergenic 25.0%
! AATAATAGAAATGTGTTGCA+AGG + chr4_4:55693996-55694015 Msa0689010:intron 25.0%
! AACAAGATTTGATCAATGTT+CGG - chr4_4:55694031-55694050 None:intergenic 25.0%
! AACATTGATCAAATCTTGTT+TGG + chr4_4:55694031-55694050 Msa0689010:intron 25.0%
! AAATGTTAGCAGAATTATGT+TGG - chr4_4:55694559-55694578 None:intergenic 25.0%
! CTTTGAACAAAGATAAAGAA+GGG - chr4_4:55694733-55694752 None:intergenic 25.0%
! ACTTTGAACAAAGATAAAGA+AGG - chr4_4:55694734-55694753 None:intergenic 25.0%
! TATGTGAATAATGTGTTAGA+TGG + chr4_4:55694803-55694822 Msa0689010:intron 25.0%
! AATCTGAACAGAGTTTATAA+AGG - chr4_4:55694914-55694933 None:intergenic 25.0%
! ACTGTTAATGTAAAGAACAT+AGG + chr4_4:55695036-55695055 Msa0689010:intron 25.0%
! TTGAATTGTATCTACCTATT+TGG + chr4_4:55695241-55695260 Msa0689010:intron 25.0%
!! AATTAACCTCTATCAACTTT+TGG + chr4_4:55695299-55695318 Msa0689010:intron 25.0%
! GAGTATAAGAAAATCTTCAA+TGG + chr4_4:55696094-55696113 Msa0689010:CDS 25.0%
AGGGAACTAATATATGATGT+TGG - chr4_4:55692739-55692758 None:intergenic 30.0%
! GAGATTTAAGGATAGCTTTT+AGG - chr4_4:55692759-55692778 None:intergenic 30.0%
ACAAAAGGAGTTCATTAGTT+TGG + chr4_4:55693183-55693202 Msa0689010:five_prime_UTR 30.0%
GTTTGGAGAATTGTATTCAA+TGG + chr4_4:55693200-55693219 Msa0689010:exon 30.0%
ATATTTGCAAAGCAGAATCA+AGG + chr4_4:55693402-55693421 Msa0689010:CDS 30.0%
TGAGTATGGTTATGTTGATA+TGG + chr4_4:55693452-55693471 Msa0689010:CDS 30.0%
ATGGTTATGTTGATATGGTT+AGG + chr4_4:55693457-55693476 Msa0689010:CDS 30.0%
TGGTTATGTTGATATGGTTA+GGG + chr4_4:55693458-55693477 Msa0689010:CDS 30.0%
TTCATATTGCTGCTAAACAA+GGG + chr4_4:55693544-55693563 Msa0689010:CDS 30.0%
! GTTCTTTTGTGTTTTGTGAA+AGG + chr4_4:55693612-55693631 Msa0689010:intron 30.0%
AATCTCAGTTCTGTTGTTAT+TGG + chr4_4:55693880-55693899 Msa0689010:intron 30.0%
AACCTATTCACTTATCAGTA+AGG - chr4_4:55693911-55693930 None:intergenic 30.0%
! TTACTACGGTTTAGTTTACA+AGG - chr4_4:55693941-55693960 None:intergenic 30.0%
GTGAAATATCTATTAGAAGC+AGG + chr4_4:55694237-55694256 Msa0689010:CDS 30.0%
AGATCCATCAACAATAAACA+TGG + chr4_4:55694461-55694480 Msa0689010:CDS 30.0%
TCAACCATGTTTATTGTTGA+TGG - chr4_4:55694468-55694487 None:intergenic 30.0%
AACTCAGGTAAATTTGACTA+TGG + chr4_4:55694533-55694552 Msa0689010:intron 30.0%
GACATGTTTATCAACAAAGA+TGG - chr4_4:55694644-55694663 None:intergenic 30.0%
CATGTCGTCACTTAAAATTT+CGG + chr4_4:55694658-55694677 Msa0689010:intron 30.0%
CTATGAAGTACAAACACTTA+CGG - chr4_4:55695130-55695149 None:intergenic 30.0%
!! CAGATTATCAAGTTGATACT+TGG + chr4_4:55695327-55695346 Msa0689010:intron 30.0%
!! AAAAGCATTCTTACAGAACA+CGG + chr4_4:55695435-55695454 Msa0689010:CDS 30.0%
! TGTTTTCGGTTGAGATTTTA+TGG - chr4_4:55695478-55695497 None:intergenic 30.0%
AGTATTCATCGTGTTTGATT+CGG + chr4_4:55695797-55695816 Msa0689010:CDS 30.0%
!!! GCTTATATCTGTGTCATTTT+TGG + chr4_4:55695938-55695957 Msa0689010:CDS 30.0%
!!! GCTTTGTCTTTTTTAGTTGT+GGG + chr4_4:55695960-55695979 Msa0689010:CDS 30.0%
!!! CTTTGTCTTTTTTAGTTGTG+GGG + chr4_4:55695961-55695980 Msa0689010:CDS 30.0%
CAATTGGGGTAACTATTATT+GGG + chr4_4:55696003-55696022 Msa0689010:CDS 30.0%
TATTATTGGGACTACCATAA+TGG + chr4_4:55696016-55696035 Msa0689010:CDS 30.0%
ATTGGGTACTATGAGTTATT+GGG + chr4_4:55696046-55696065 Msa0689010:CDS 30.0%
CTATGAGTTATTGGGTTATT+AGG + chr4_4:55696054-55696073 Msa0689010:CDS 30.0%
TTAGGCATAGAATCAATTTG+AGG + chr4_4:55696072-55696091 Msa0689010:CDS 30.0%
CTAATATATGATGTTGGCGT+AGG - chr4_4:55692733-55692752 None:intergenic 35.0%
TTTAGTGGAACACACAAGTA+AGG + chr4_4:55692787-55692806 Msa0689010:five_prime_UTR 35.0%
!! AGAGCAATGATTTTGGAGAA+AGG + chr4_4:55692958-55692977 Msa0689010:intron 35.0%
GAAAATGACGAAGCAATTGA+CGG + chr4_4:55693278-55693297 Msa0689010:CDS 35.0%
AAAATGACGAAGCAATTGAC+GGG + chr4_4:55693279-55693298 Msa0689010:CDS 35.0%
! TCTTTGAAGGATACAGTTGA+TGG + chr4_4:55693354-55693373 Msa0689010:CDS 35.0%
TATTATGATCTTGCTGATGC+TGG + chr4_4:55693492-55693511 Msa0689010:CDS 35.0%
GCTGGAATTAAAGCTAGAAA+TGG + chr4_4:55693510-55693529 Msa0689010:CDS 35.0%
CTTCATATTGCTGCTAAACA+AGG + chr4_4:55693543-55693562 Msa0689010:CDS 35.0%
TCATATTGCTGCTAAACAAG+GGG + chr4_4:55693545-55693564 Msa0689010:CDS 35.0%
ACAAGGGGATTTAGGTATTA+TGG + chr4_4:55693560-55693579 Msa0689010:intron 35.0%
TTTGTGAAAGGAGTAATGAG+TGG + chr4_4:55693624-55693643 Msa0689010:intron 35.0%
TGTCTTCGCAATCATAATTG+AGG - chr4_4:55693734-55693753 None:intergenic 35.0%
GTGTCATAAAAATCCACTCA+AGG - chr4_4:55693813-55693832 None:intergenic 35.0%
AAAGCACAAAATGAGCAACT+TGG - chr4_4:55693835-55693854 None:intergenic 35.0%
TGCCTTACTGATAAGTGAAT+AGG + chr4_4:55693906-55693925 Msa0689010:intron 35.0%
AAACTGTTAGAACAAAACCC+TGG - chr4_4:55694099-55694118 None:intergenic 35.0%
TTCTGAATTGTCAATGACTG+TGG + chr4_4:55694158-55694177 Msa0689010:CDS 35.0%
GCAACAATTGCTAGAAGTAA+TGG + chr4_4:55694270-55694289 Msa0689010:CDS 35.0%
AAGATAGTGTTCACAAGAAG+TGG + chr4_4:55694602-55694621 Msa0689010:intron 35.0%
ATCTCACAAACTGTGTTTGT+AGG - chr4_4:55694700-55694719 None:intergenic 35.0%
TCTTTGTTCAAAGTGTGACT+TGG + chr4_4:55694740-55694759 Msa0689010:intron 35.0%
CTTTGTTCAAAGTGTGACTT+GGG + chr4_4:55694741-55694760 Msa0689010:intron 35.0%
! GTATGTGTAAGGGGTATATA+GGG - chr4_4:55694768-55694787 None:intergenic 35.0%
!! CCAATTTTGGTATGTGTAAG+GGG - chr4_4:55694777-55694796 None:intergenic 35.0%
!! CCCAATTTTGGTATGTGTAA+GGG - chr4_4:55694778-55694797 None:intergenic 35.0%
CCCTTACACATACCAAAATT+GGG + chr4_4:55694775-55694794 Msa0689010:intron 35.0%
TTGGTACTATCCTAGAACTT+GGG + chr4_4:55694840-55694859 Msa0689010:intron 35.0%
AGTGCTTTGTAGGATAATAG+CGG - chr4_4:55694970-55694989 None:intergenic 35.0%
TGTCAATGTCGTATATGTGT+CGG - chr4_4:55694997-55695016 None:intergenic 35.0%
ATGTAAAGAACATAGGACAC+CGG + chr4_4:55695043-55695062 Msa0689010:intron 35.0%
TGAACTCGTATGATACCTAT+TGG - chr4_4:55695071-55695090 None:intergenic 35.0%
GTACAAACACTTACGGAATT+AGG - chr4_4:55695123-55695142 None:intergenic 35.0%
ACTTCATAGCTGCTAATTCT+TGG + chr4_4:55695141-55695160 Msa0689010:intron 35.0%
CTTCATAGCTGCTAATTCTT+GGG + chr4_4:55695142-55695161 Msa0689010:intron 35.0%
TTCATAGCTGCTAATTCTTG+GGG + chr4_4:55695143-55695162 Msa0689010:intron 35.0%
TACCTATTTGGATTAAGTGC+TGG + chr4_4:55695253-55695272 Msa0689010:intron 35.0%
ATCCAGCACTTAATCCAAAT+AGG - chr4_4:55695258-55695277 None:intergenic 35.0%
!! GATTAAGTGCTGGATTATTC+AGG + chr4_4:55695263-55695282 Msa0689010:intron 35.0%
!! GCATTTCCAAAAGTTGATAG+AGG - chr4_4:55695308-55695327 None:intergenic 35.0%
CTTGGACAAAGTGAAACAAA+TGG + chr4_4:55695345-55695364 Msa0689010:CDS 35.0%
ACAAAGTGAAACAAATGGCA+TGG + chr4_4:55695350-55695369 Msa0689010:CDS 35.0%
CAAAGCAAAGAAGCAAATGA+TGG + chr4_4:55695881-55695900 Msa0689010:CDS 35.0%
!!! GGCTTTGTCTTTTTTAGTTG+TGG + chr4_4:55695959-55695978 Msa0689010:CDS 35.0%
TAGTTGTGGGGAAAAAACAA+AGG + chr4_4:55695973-55695992 Msa0689010:CDS 35.0%
GCAATTGGGGTAACTATTAT+TGG + chr4_4:55696002-55696021 Msa0689010:CDS 35.0%
TACCATAATGGCTACAACAT+TGG + chr4_4:55696028-55696047 Msa0689010:CDS 35.0%
ACCATAATGGCTACAACATT+GGG + chr4_4:55696029-55696048 Msa0689010:CDS 35.0%
CATTGGGTACTATGAGTTAT+TGG + chr4_4:55696045-55696064 Msa0689010:CDS 35.0%
GAAAATCTTCAATGGGAAGT+AGG + chr4_4:55696102-55696121 Msa0689010:CDS 35.0%
!! CACTACCTTTTGTTTCCTGT+TGG + chr4_4:55692411-55692430 Msa0689010:five_prime_UTR 40.0%
ATGCACCAACAGGAAACAAA+AGG - chr4_4:55692419-55692438 None:intergenic 40.0%
GATATACATCATGCACCAAC+AGG - chr4_4:55692429-55692448 None:intergenic 40.0%
AAATGACGAAGCAATTGACG+GGG + chr4_4:55693280-55693299 Msa0689010:CDS 40.0%
TTTGCAAAGCAGAATCAAGG+TGG + chr4_4:55693405-55693424 Msa0689010:CDS 40.0%
CTTTATGTTGCTGCTGAGTA+TGG + chr4_4:55693438-55693457 Msa0689010:CDS 40.0%
ATTAGAAGCAGGTAGTAGCT+TGG + chr4_4:55694248-55694267 Msa0689010:CDS 40.0%
TTGCATTCTGCTGCAAGAAA+TGG + chr4_4:55694303-55694322 Msa0689010:CDS 40.0%
TGCTGCAAGAAATGGACATT+TGG + chr4_4:55694311-55694330 Msa0689010:CDS 40.0%
TGCAAGAAATGGACATTTGG+AGG + chr4_4:55694314-55694333 Msa0689010:CDS 40.0%
TGTGAAAGCGATTCTTGAGA+AGG + chr4_4:55694338-55694357 Msa0689010:CDS 40.0%
! TGTAACGCGGACTGATAAAA+AGG + chr4_4:55694371-55694390 Msa0689010:CDS 40.0%
! GTAACGCGGACTGATAAAAA+GGG + chr4_4:55694372-55694391 Msa0689010:CDS 40.0%
GACTGATAAAAAGGGACAGA+CGG + chr4_4:55694380-55694399 Msa0689010:CDS 40.0%
TTGCATATAGCAACCAGGAA+GGG + chr4_4:55694507-55694526 Msa0689010:CDS 40.0%
! GGTATGTGTAAGGGGTATAT+AGG - chr4_4:55694769-55694788 None:intergenic 40.0%
CCCCTTACACATACCAAAAT+TGG + chr4_4:55694774-55694793 Msa0689010:intron 40.0%
!! GCCCAATTTTGGTATGTGTA+AGG - chr4_4:55694779-55694798 None:intergenic 40.0%
ACACATACCAAAATTGGGCT+TGG + chr4_4:55694780-55694799 Msa0689010:intron 40.0%
! TCACATACCAAGCCCAATTT+TGG - chr4_4:55694790-55694809 None:intergenic 40.0%
TGTTAGATGGCTTGATGAAC+AGG + chr4_4:55694816-55694835 Msa0689010:intron 40.0%
CTTGGTACTATCCTAGAACT+TGG + chr4_4:55694839-55694858 Msa0689010:intron 40.0%
TTAAGCACAACCCAAGTTCT+AGG - chr4_4:55694853-55694872 None:intergenic 40.0%
TGCTTTGTAGGATAATAGCG+GGG - chr4_4:55694968-55694987 None:intergenic 40.0%
GTGCTTTGTAGGATAATAGC+GGG - chr4_4:55694969-55694988 None:intergenic 40.0%
!! TCTCTTGTTGCAGCTGTTTT+CGG - chr4_4:55695492-55695511 None:intergenic 40.0%
TTTGACGAGTGTGTTCTAGT+TGG - chr4_4:55695557-55695576 None:intergenic 40.0%
CTCAACAAAATGCACACAGA+AGG + chr4_4:55695609-55695628 Msa0689010:CDS 40.0%
!! TTTCCTGGAGGTATGTGTTT+TGG - chr4_4:55695735-55695754 None:intergenic 40.0%
!!! AGCTATTTTTGCTTCGCCAA+GGG - chr4_4:55695760-55695779 None:intergenic 40.0%
!!! AAGCTATTTTTGCTTCGCCA+AGG - chr4_4:55695761-55695780 None:intergenic 40.0%
ACGATGAATACTAGAAACGC+GGG - chr4_4:55695789-55695808 None:intergenic 40.0%
CACGATGAATACTAGAAACG+CGG - chr4_4:55695790-55695809 None:intergenic 40.0%
TTGTGGGGAAAAAACAAAGG+TGG + chr4_4:55695976-55695995 Msa0689010:CDS 40.0%
! AAACAAAGGTGGCTTGCAAT+TGG + chr4_4:55695987-55696006 Msa0689010:CDS 40.0%
! AACAAAGGTGGCTTGCAATT+GGG + chr4_4:55695988-55696007 Msa0689010:CDS 40.0%
ACCCAATGTTGTAGCCATTA+TGG - chr4_4:55696033-55696052 None:intergenic 40.0%
TTCTTCCTTTGAGCCCTTAC+TGG - chr4_4:55693261-55693280 None:intergenic 45.0%
CGATGATACGCCGTTACATT+CGG + chr4_4:55693308-55693327 Msa0689010:CDS 45.0%
TGGGAACATGGCTTCTTTGA+AGG + chr4_4:55693341-55693360 Msa0689010:CDS 45.0%
!! TACAGTTGATGGTGCAGAAG+AGG + chr4_4:55693365-55693384 Msa0689010:CDS 45.0%
!! ACAGTTGATGGTGCAGAAGA+GGG + chr4_4:55693366-55693385 Msa0689010:CDS 45.0%
GCTGCTAAACAAGGGGATTT+AGG + chr4_4:55693552-55693571 Msa0689010:CDS 45.0%
TTGCGGTTGAAGTCATGTTG+CGG + chr4_4:55693664-55693683 Msa0689010:intron 45.0%
GTTGCATATAGCAACCAGGA+AGG + chr4_4:55694506-55694525 Msa0689010:CDS 45.0%
CGTATGATACCTATTGGTGC+CGG - chr4_4:55695065-55695084 None:intergenic 45.0%
GGCATGGCAGTAAACAAAAG+TGG + chr4_4:55695366-55695385 Msa0689010:CDS 45.0%
CGAAACAGCATTAGACACAG+CGG + chr4_4:55695389-55695408 Msa0689010:CDS 45.0%
TTAGACACAGCGGAGAAAAC+AGG + chr4_4:55695399-55695418 Msa0689010:CDS 45.0%
!! GAGTGTGTTCTAGTTGGTGA+TGG - chr4_4:55695551-55695570 None:intergenic 45.0%
!! CAAACACGAAGAAGCGTTCA+AGG + chr4_4:55695573-55695592 Msa0689010:CDS 45.0%
!! GTTTTGGATCGTCCACGAAT+TGG - chr4_4:55695719-55695738 None:intergenic 45.0%
GATCCAAAACACATACCTCC+AGG + chr4_4:55695729-55695748 Msa0689010:CDS 45.0%
ATACCTCCAGGAAAATCCCT+TGG + chr4_4:55695741-55695760 Msa0689010:CDS 45.0%
! ACAAAGGTGGCTTGCAATTG+GGG + chr4_4:55695989-55696008 Msa0689010:CDS 45.0%
CTTCAATGGGAAGTAGGTCA+AGG + chr4_4:55696108-55696127 Msa0689010:CDS 45.0%
!! TGTTAATATATTAATATTAA+AGG + chr4_4:55692684-55692703 Msa0689010:intron 5.0%
GCTCTTGCTGCCGAATGTAA+CGG - chr4_4:55693321-55693340 None:intergenic 50.0%
TTACATTCGGCAGCAAGAGC+TGG + chr4_4:55693321-55693340 Msa0689010:CDS 50.0%
TACATTCGGCAGCAAGAGCT+GGG + chr4_4:55693322-55693341 Msa0689010:CDS 50.0%
TGCAAGGCTGTTGTGTTGGA+CGG - chr4_4:55694186-55694205 None:intergenic 50.0%
AGTGTGCAAGGCTGTTGTGT+TGG - chr4_4:55694190-55694209 None:intergenic 50.0%
TTGTGTTGCAGCAGTGTGCA+AGG - chr4_4:55694202-55694221 None:intergenic 50.0%
TTGCACACTGCTGCAACACA+AGG + chr4_4:55694201-55694220 Msa0689010:CDS 50.0%
! GCAGAGTCTTGTGGTAGTAG+AGG + chr4_4:55694425-55694444 Msa0689010:CDS 50.0%
CAGCGTTGCATATAGCAACC+AGG + chr4_4:55694502-55694521 Msa0689010:CDS 50.0%
AACCAGGAAGGGAAGAACTC+AGG + chr4_4:55694518-55694537 Msa0689010:CDS 50.0%
TACCTGAGTTCTTCCCTTCC+TGG - chr4_4:55694523-55694542 None:intergenic 50.0%
GATGGCTTGATGAACAGGCT+TGG + chr4_4:55694821-55694840 Msa0689010:intron 50.0%
TGTCGGAGTCAGTGCTTTGT+AGG - chr4_4:55694980-55694999 None:intergenic 50.0%
AAACTCCACAACAGTCGTCG+CGG + chr4_4:55695647-55695666 Msa0689010:CDS 50.0%
CGAAAGCAACCGTTGCTATG+AGG - chr4_4:55695674-55695693 None:intergenic 50.0%
AGCTGAAACTGTGCAGCCTC+AGG + chr4_4:55693230-55693249 Msa0689010:CDS 55.0%
CTGCACCAGTAAGGGCTCAA+AGG + chr4_4:55693253-55693272 Msa0689010:CDS 55.0%
GCGATTCTTGAGAAGGAGCC+AGG + chr4_4:55694345-55694364 Msa0689010:CDS 55.0%
! AGGAGCCAGGTGTTGTAACG+CGG + chr4_4:55694358-55694377 Msa0689010:CDS 55.0%
TCAGTCCGCGTTACAACACC+TGG - chr4_4:55694366-55694385 None:intergenic 55.0%
GCGCTTCACATGGCTGTGAA+GGG + chr4_4:55694402-55694421 Msa0689010:CDS 55.0%
CGCTTCACATGGCTGTGAAG+GGG + chr4_4:55694403-55694422 Msa0689010:CDS 55.0%
TGTGAAGGGGCAGAGTCTTG+TGG + chr4_4:55694416-55694435 Msa0689010:CDS 55.0%
CATAGGACACCGGCACCAAT+AGG + chr4_4:55695053-55695072 Msa0689010:intron 55.0%
! GATCGTCCACGAATTGGCCT+GGG - chr4_4:55695713-55695732 None:intergenic 55.0%
! TCGCCAAGGGATTTTCCTGG+AGG - chr4_4:55695747-55695766 None:intergenic 55.0%
! GCTTCGCCAAGGGATTTTCC+TGG - chr4_4:55695750-55695769 None:intergenic 55.0%
TTTCATCTCCCTCGCGGTTG+TGG + chr4_4:55695827-55695846 Msa0689010:CDS 55.0%
TTGAACCACCACAACCGCGA+GGG - chr4_4:55695838-55695857 None:intergenic 55.0%
TTTGAACCACCACAACCGCG+AGG - chr4_4:55695839-55695858 None:intergenic 55.0%
AGCCTCAGGCTGCACCAGTA+AGG + chr4_4:55693244-55693263 Msa0689010:CDS 60.0%
GCCTCAGGCTGCACCAGTAA+GGG + chr4_4:55693245-55693264 Msa0689010:CDS 60.0%
GGCAGCAAGAGCTGGGAACA+TGG + chr4_4:55693329-55693348 Msa0689010:CDS 60.0%
GGCGCTTCACATGGCTGTGA+AGG + chr4_4:55694401-55694420 Msa0689010:CDS 60.0%
CGTCGCGGTCCTCATAGCAA+CGG + chr4_4:55695662-55695681 Msa0689010:CDS 60.0%
TTGGCCTGGGACGGTGAAGA+TGG - chr4_4:55695700-55695719 None:intergenic 60.0%
! GTCCACGAATTGGCCTGGGA+CGG - chr4_4:55695709-55695728 None:intergenic 60.0%
GGATCGTCCACGAATTGGCC+TGG - chr4_4:55695714-55695733 None:intergenic 60.0%
! CGCGCTTTTCATCTCCCTCG+CGG + chr4_4:55695821-55695840 Msa0689010:CDS 60.0%
GCCCTTACTGGTGCAGCCTG+AGG - chr4_4:55693249-55693268 None:intergenic 65.0%
GGGACAGACGGCGCTTCACA+TGG + chr4_4:55694392-55694411 Msa0689010:CDS 65.0%
GAGGACCGCGACGACTGTTG+TGG - chr4_4:55695655-55695674 None:intergenic 65.0%
GCAGCCATCTTCACCGTCCC+AGG + chr4_4:55695693-55695712 Msa0689010:CDS 65.0%
CACCGTCCCAGGCCAATTCG+TGG + chr4_4:55695704-55695723 Msa0689010:CDS 65.0%
CATCTCCCTCGCGGTTGTGG+TGG + chr4_4:55695830-55695849 Msa0689010:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 55692319 55696258 55692319 ID=Msa0689010;Name=Msa0689010
chr4_4 mRNA 55692319 55696258 55692319 ID=Msa0689010-mRNA-1;Parent=Msa0689010;Name=Msa0689010-mRNA-1;_AED=0.12;_eAED=0.12;_QI=438|1|1|1|0.4|0.33|6|53|546
chr4_4 exon 55692319 55692544 55692319 ID=Msa0689010-mRNA-1:exon:14642;Parent=Msa0689010-mRNA-1
chr4_4 exon 55692725 55692808 55692725 ID=Msa0689010-mRNA-1:exon:14643;Parent=Msa0689010-mRNA-1
chr4_4 exon 55692960 55693007 55692960 ID=Msa0689010-mRNA-1:exon:14644;Parent=Msa0689010-mRNA-1
chr4_4 exon 55693139 55693573 55693139 ID=Msa0689010-mRNA-1:exon:14645;Parent=Msa0689010-mRNA-1
chr4_4 exon 55694130 55694539 55694130 ID=Msa0689010-mRNA-1:exon:14646;Parent=Msa0689010-mRNA-1
chr4_4 exon 55695330 55696258 55695330 ID=Msa0689010-mRNA-1:exon:14647;Parent=Msa0689010-mRNA-1
chr4_4 five_prime_UTR 55692319 55692544 55692319 ID=Msa0689010-mRNA-1:five_prime_utr;Parent=Msa0689010-mRNA-1
chr4_4 five_prime_UTR 55692725 55692808 55692725 ID=Msa0689010-mRNA-1:five_prime_utr;Parent=Msa0689010-mRNA-1
chr4_4 five_prime_UTR 55692960 55693007 55692960 ID=Msa0689010-mRNA-1:five_prime_utr;Parent=Msa0689010-mRNA-1
chr4_4 five_prime_UTR 55693139 55693218 55693139 ID=Msa0689010-mRNA-1:five_prime_utr;Parent=Msa0689010-mRNA-1
chr4_4 CDS 55693219 55693573 55693219 ID=Msa0689010-mRNA-1:cds;Parent=Msa0689010-mRNA-1
chr4_4 CDS 55694130 55694539 55694130 ID=Msa0689010-mRNA-1:cds;Parent=Msa0689010-mRNA-1
chr4_4 CDS 55695330 55696205 55695330 ID=Msa0689010-mRNA-1:cds;Parent=Msa0689010-mRNA-1
chr4_4 three_prime_UTR 55696206 55696258 55696206 ID=Msa0689010-mRNA-1:three_prime_utr;Parent=Msa0689010-mRNA-1
Gene Sequence

>Msa0689010

ATGGACAAAGAAGCTGAAACTGTGCAGCCTCAGGCTGCACCAGTAAGGGCTCAAAGGAAGAAAATGACGAAGCAATTGACGGGGAAGCGCGATGATACGCCGTTACATTCGGCAGCAAGAGCTGGGAACATGGCTTCTTTGAAGGATACAGTTGATGGTGCAGAAGAGGGTAAACTGCGCGAAATATTTGCAAAGCAGAATCAAGGTGGAGAAACAGCACTTTATGTTGCTGCTGAGTATGGTTATGTTGATATGGTTAGGGAGATGATTCAGTATTATGATCTTGCTGATGCTGGAATTAAAGCTAGAAATGGTTTTGATGCACTTCATATTGCTGCTAAACAAGGGGATTTAGATATTGTAAAGATCTTAATGGAAGCTCATTCTGAATTGTCAATGACTGTGGATCCGTCCAACACAACAGCCTTGCACACTGCTGCAACACAAGGACATACTGAGATAGTGAAATATCTATTAGAAGCAGGTAGTAGCTTGGCAACAATTGCTAGAAGTAATGGTAAAACAGCTTTGCATTCTGCTGCAAGAAATGGACATTTGGAGGTTGTGAAAGCGATTCTTGAGAAGGAGCCAGGTGTTGTAACGCGGACTGATAAAAAGGGACAGACGGCGCTTCACATGGCTGTGAAGGGGCAGAGTCTTGTGGTAGTAGAGGAGTTGATAAAAGCAGATCCATCAACAATAAACATGGTTGATAATAAGGGTAATACAGCGTTGCATATAGCAACCAGGAAGGGAAGAACTCAGATTATCAAGTTGATACTTGGACAAAGTGAAACAAATGGCATGGCAGTAAACAAAAGTGGCGAAACAGCATTAGACACAGCGGAGAAAACAGGAAACTCTGAAGTAAAAAGCATTCTTACAGAACACGGTGTTCAAAGCAGCAAATCCATAAAATCTCAACCGAAAACAGCTGCAACAAGAGAATTAAAACAAACAGTCAGTGACATAAAGCATGAAGTCCATCACCAACTAGAACACACTCGTCAAACACGAAGAAGCGTTCAAGGAATAGCAAAACGTCTCAACAAAATGCACACAGAAGGACTCAACAATGCGATAAACTCCACAACAGTCGTCGCGGTCCTCATAGCAACGGTTGCTTTCGCAGCCATCTTCACCGTCCCAGGCCAATTCGTGGACGATCCAAAACACATACCTCCAGGAAAATCCCTTGGCGAAGCAAAAATAGCTTCAGAACCCGCGTTTCTAGTATTCATCGTGTTTGATTCGGTCGCGCTTTTCATCTCCCTCGCGGTTGTGGTGGTTCAAACATCAATAGTTGTTGTTGAAAGCAAAGCAAAGAAGCAAATGATGGCTATTATTAACAAACTAATGTGGTTAGCTTGTGTGCTTATATCTGTGTCATTTTTGGCTTTGTCTTTTTTAGTTGTGGGGAAAAAACAAAGGTGGCTTGCAATTGGGGTAACTATTATTGGGACTACCATAATGGCTACAACATTGGGTACTATGAGTTATTGGGTTATTAGGCATAGAATCAATTTGAGGAGTATAAGAAAATCTTCAATGGGAAGTAGGTCAAGGTCTTTTTCAGTTTCAGTTGTTATGTCTGATTCTGAGATATTAAACAATGAGCGTAATAAAATGTATGCTATTTAG
Protein sequence

>Msa0689010

MDKEAETVQPQAAPVRAQRKKMTKQLTGKRDDTPLHSAARAGNMASLKDTVDGAEEGKLREIFAKQNQGGETALYVAAEYGYVDMVREMIQYYDLADAGIKARNGFDALHIAAKQGDLDIVKILMEAHSELSMTVDPSNTTALHTAATQGHTEIVKYLLEAGSSLATIARSNGKTALHSAARNGHLEVVKAILEKEPGVVTRTDKKGQTALHMAVKGQSLVVVEELIKADPSTINMVDNKGNTALHIATRKGRTQIIKLILGQSETNGMAVNKSGETALDTAEKTGNSEVKSILTEHGVQSSKSIKSQPKTAATRELKQTVSDIKHEVHHQLEHTRQTRRSVQGIAKRLNKMHTEGLNNAINSTTVVAVLIATVAFAAIFTVPGQFVDDPKHIPPGKSLGEAKIASEPAFLVFIVFDSVALFISLAVVVVQTSIVVVESKAKKQMMAIINKLMWLACVLISVSFLALSFLVVGKKQRWLAIGVTIIGTTIMATTLGTMSYWVIRHRINLRSIRKSSMGSRSRSFSVSVVMSDSEILNNERNKMYAI*