AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1357490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357490 MtrunA17_Chr3g0124301 99.375 160 1 0 1 160 1 160 3.08e-119 333
Msa1357490 MtrunA17_Chr3g0115561 39.860 143 78 3 16 152 13 153 3.66e-31 110
Msa1357490 MtrunA17_Chr2g0283801 34.848 132 78 5 25 152 28 155 1.39e-22 88.2
Msa1357490 MtrunA17_Chr2g0316111 27.160 162 99 4 4 151 8 164 1.36e-13 65.1
Msa1357490 MtrunA17_Chr2g0316151 28.402 169 98 6 1 151 1 164 1.86e-13 64.7
Msa1357490 MtrunA17_Chr2g0316121 28.667 150 89 6 1 137 1 145 7.76e-11 57.4
Msa1357490 MtrunA17_Chr2g0316161 28.667 150 89 6 1 137 1 145 8.09e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357490 AT5G10130.1 46.914 162 81 3 1 159 1 160 3.29e-45 146
Msa1357490 AT4G08685.1 40.994 161 84 4 5 159 4 159 5.29e-36 122
Msa1357490 AT1G78040.1 36.095 169 92 5 1 159 1 163 5.96e-34 117
Msa1357490 AT1G78040.3 36.095 169 92 5 1 159 1 163 5.96e-34 117
Msa1357490 AT1G78040.2 36.095 169 92 5 1 159 1 163 5.96e-34 117
Msa1357490 AT1G29140.1 35.669 157 92 3 4 152 6 161 1.18e-26 99.0
Msa1357490 AT5G45880.1 39.024 123 70 3 25 143 35 156 4.04e-23 90.1
Msa1357490 AT4G18596.1 37.705 122 71 3 25 142 33 153 2.65e-22 87.8
Msa1357490 AT4G18596.2 37.705 122 71 3 25 142 79 199 6.63e-22 88.2

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGATTGTGTTGCTGTTAA+TGG 0.278593 tig0016091:+4355 Msa1357490:CDS
AATAAACACAACCTTTGATA+TGG 0.316996 tig0016091:-4424 None:intergenic
GTGTATTACACGATGATATT+GGG 0.373900 tig0016091:-4874 None:intergenic
GGTGTATTACACGATGATAT+TGG 0.406710 tig0016091:-4875 None:intergenic
CACTGGAACATACAACATAA+TGG 0.429786 tig0016091:+4802 Msa1357490:CDS
CGTCCATAGTCTTCCTGTCT+TGG 0.431661 tig0016091:-4748 None:intergenic
ACAAGTAATATTTGAGGAGT+TGG 0.437901 tig0016091:-5021 None:intergenic
AAATGCTATGGGTTACTTCA+AGG 0.456909 tig0016091:+4973 Msa1357490:CDS
TAGGTGCAAAGGTGGGAATT+CGG 0.460306 tig0016091:+4723 Msa1357490:intron
TGGGAATTCGGTGCCAAGAC+AGG 0.469322 tig0016091:+4735 Msa1357490:CDS
TATTGTGATACTTGCCGTGC+TGG 0.471219 tig0016091:+4443 Msa1357490:CDS
GTGCCAAGACAGGAAGACTA+TGG 0.475430 tig0016091:+4745 Msa1357490:CDS
TCTTCACTATGCAAATGCTA+TGG 0.481660 tig0016091:+4961 Msa1357490:CDS
GGAGGATAGAGCTGAGGGAA+AGG 0.482593 tig0016091:-4379 None:intergenic
TAGCATTTGCATAGTGAAGA+TGG 0.484441 tig0016091:-4958 None:intergenic
GATCTGTTACGCCCAGGGTC+CGG 0.486304 tig0016091:-4896 None:intergenic
AACATAGCTGTGGCAAGTAA+AGG 0.486675 tig0016091:-4401 None:intergenic
ACATAGCTGTGGCAAGTAAA+GGG 0.489307 tig0016091:-4400 None:intergenic
GAGGATAGAGCTGAGGGAAA+GGG 0.496587 tig0016091:-4378 None:intergenic
AGGAGTTGGTGGCAATATTG+AGG 0.508425 tig0016091:-5007 None:intergenic
AATCAGACAAGTAATATTTG+AGG 0.510401 tig0016091:-5027 None:intergenic
TGCTGGATCTGTTACGCCCA+GGG 0.512778 tig0016091:-4901 None:intergenic
AGTAATATTTGAGGAGTTGG+TGG 0.513246 tig0016091:-5018 None:intergenic
GTAGTGAGTGAGCACAATGC+TGG 0.521929 tig0016091:-4918 None:intergenic
ATGCTGGATCTGTTACGCCC+AGG 0.535067 tig0016091:-4902 None:intergenic
CTTCACTATGCAAATGCTAT+GGG 0.535954 tig0016091:+4962 Msa1357490:CDS
TCGTGTAATACACCGGACCC+TGG 0.543647 tig0016091:+4884 Msa1357490:CDS
TTGCATAGTGAAGATGGTTG+AGG 0.549905 tig0016091:-4952 None:intergenic
TTCTTGATTAGGTGCAAAGG+TGG 0.550705 tig0016091:+4715 Msa1357490:intron
CGTGTAATACACCGGACCCT+GGG 0.556675 tig0016091:+4885 Msa1357490:CDS
TGGTGGCAATATTGAGGCAA+AGG 0.557338 tig0016091:-5001 None:intergenic
CATTATGTTGTATGTTCCAG+TGG 0.561706 tig0016091:-4801 None:intergenic
CATTGCTAATCATCATGGCA+AGG 0.587013 tig0016091:+4336 None:intergenic
CGATTCTTGATTAGGTGCAA+AGG 0.587702 tig0016091:+4712 Msa1357490:intron
ACAGCTATGTTCCATATCAA+AGG 0.587960 tig0016091:+4413 Msa1357490:CDS
TAAAGGGAGGATAGAGCTGA+GGG 0.597004 tig0016091:-4384 None:intergenic
GTAAAGGGAGGATAGAGCTG+AGG 0.612679 tig0016091:-4385 None:intergenic
GAAGGAGTGACAGATTCCAC+TGG 0.617675 tig0016091:+4785 Msa1357490:CDS
TTGGATGTAAAATGTGGCGT+TGG 0.634933 tig0016091:-4474 None:intergenic
CGTTGGTTTCGAATCCAGCA+CGG 0.637233 tig0016091:-4457 None:intergenic
TCTTGATTAGGTGCAAAGGT+GGG 0.642740 tig0016091:+4716 Msa1357490:intron
AATATCATCGTGTAATACAC+CGG 0.667061 tig0016091:+4877 Msa1357490:CDS
TTTACCTTGGATGTAAAATG+TGG 0.673598 tig0016091:-4480 None:intergenic
TTTGATATGGAACATAGCTG+TGG 0.697489 tig0016091:-4411 None:intergenic
TAGCTGTGGCAAGTAAAGGG+AGG 0.702643 tig0016091:-4397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTAAAAAATTTACGTTA+AGG - tig0016091:4512-4531 None:intergenic 15.0%
! AATAAACACAACCTTTGATA+TGG - tig0016091:4427-4446 None:intergenic 25.0%
! AATCAGACAAGTAATATTTG+AGG - tig0016091:5030-5049 None:intergenic 25.0%
TTTACCTTGGATGTAAAATG+TGG - tig0016091:4483-4502 None:intergenic 30.0%
TGTAAAATGTACACATGATC+AGG - tig0016091:4583-4602 None:intergenic 30.0%
TATTGTGACGATTCTTGATT+AGG + tig0016091:4704-4723 Msa1357490:intron 30.0%
! CAGTTTTGCAAATATTGTCA+TGG - tig0016091:4841-4860 None:intergenic 30.0%
GTGTATTACACGATGATATT+GGG - tig0016091:4877-4896 None:intergenic 30.0%
AATATCATCGTGTAATACAC+CGG + tig0016091:4877-4896 Msa1357490:CDS 30.0%
ACAAGTAATATTTGAGGAGT+TGG - tig0016091:5024-5043 None:intergenic 30.0%
! AAGGATTGTGTTGCTGTTAA+TGG + tig0016091:4355-4374 Msa1357490:CDS 35.0%
TTTGATATGGAACATAGCTG+TGG - tig0016091:4414-4433 None:intergenic 35.0%
ACAGCTATGTTCCATATCAA+AGG + tig0016091:4413-4432 Msa1357490:CDS 35.0%
GTTAAGGTGTGTATTTACCT+TGG - tig0016091:4496-4515 None:intergenic 35.0%
! TATGGACGAAGTTTTCTACA+CGG + tig0016091:4763-4782 Msa1357490:CDS 35.0%
CATTATGTTGTATGTTCCAG+TGG - tig0016091:4804-4823 None:intergenic 35.0%
CACTGGAACATACAACATAA+TGG + tig0016091:4802-4821 Msa1357490:CDS 35.0%
GGTGTATTACACGATGATAT+TGG - tig0016091:4878-4897 None:intergenic 35.0%
TAGCATTTGCATAGTGAAGA+TGG - tig0016091:4961-4980 None:intergenic 35.0%
TCTTCACTATGCAAATGCTA+TGG + tig0016091:4961-4980 Msa1357490:CDS 35.0%
CTTCACTATGCAAATGCTAT+GGG + tig0016091:4962-4981 Msa1357490:CDS 35.0%
! AAATGCTATGGGTTACTTCA+AGG + tig0016091:4973-4992 Msa1357490:CDS 35.0%
AGTAATATTTGAGGAGTTGG+TGG - tig0016091:5021-5040 None:intergenic 35.0%
ACATAGCTGTGGCAAGTAAA+GGG - tig0016091:4403-4422 None:intergenic 40.0%
AACATAGCTGTGGCAAGTAA+AGG - tig0016091:4404-4423 None:intergenic 40.0%
TTGGATGTAAAATGTGGCGT+TGG - tig0016091:4477-4496 None:intergenic 40.0%
! AACGCCACATTTTACATCCA+AGG + tig0016091:4476-4495 Msa1357490:CDS 40.0%
CGATTCTTGATTAGGTGCAA+AGG + tig0016091:4712-4731 Msa1357490:intron 40.0%
TTCTTGATTAGGTGCAAAGG+TGG + tig0016091:4715-4734 Msa1357490:intron 40.0%
TCTTGATTAGGTGCAAAGGT+GGG + tig0016091:4716-4735 Msa1357490:intron 40.0%
GTGCTCACTCACTACAAAAA+TGG + tig0016091:4926-4945 Msa1357490:CDS 40.0%
TTGCATAGTGAAGATGGTTG+AGG - tig0016091:4955-4974 None:intergenic 40.0%
TAAAGGGAGGATAGAGCTGA+GGG - tig0016091:4387-4406 None:intergenic 45.0%
TATTGTGATACTTGCCGTGC+TGG + tig0016091:4443-4462 Msa1357490:CDS 45.0%
TAGGTGCAAAGGTGGGAATT+CGG + tig0016091:4723-4742 Msa1357490:intron 45.0%
! GACGAAGTTTTCTACACGGA+AGG + tig0016091:4767-4786 Msa1357490:CDS 45.0%
TGGTGGCAATATTGAGGCAA+AGG - tig0016091:5004-5023 None:intergenic 45.0%
!! AGGAGTTGGTGGCAATATTG+AGG - tig0016091:5010-5029 None:intergenic 45.0%
GAGGATAGAGCTGAGGGAAA+GGG - tig0016091:4381-4400 None:intergenic 50.0%
GTAAAGGGAGGATAGAGCTG+AGG - tig0016091:4388-4407 None:intergenic 50.0%
TAGCTGTGGCAAGTAAAGGG+AGG - tig0016091:4400-4419 None:intergenic 50.0%
!! CGTTGGTTTCGAATCCAGCA+CGG - tig0016091:4460-4479 None:intergenic 50.0%
GTGCCAAGACAGGAAGACTA+TGG + tig0016091:4745-4764 Msa1357490:CDS 50.0%
CGTCCATAGTCTTCCTGTCT+TGG - tig0016091:4751-4770 None:intergenic 50.0%
GAAGGAGTGACAGATTCCAC+TGG + tig0016091:4785-4804 Msa1357490:CDS 50.0%
GTAGTGAGTGAGCACAATGC+TGG - tig0016091:4921-4940 None:intergenic 50.0%
GGAGGATAGAGCTGAGGGAA+AGG - tig0016091:4382-4401 None:intergenic 55.0%
!! TGGGAATTCGGTGCCAAGAC+AGG + tig0016091:4735-4754 Msa1357490:CDS 55.0%
TCGTGTAATACACCGGACCC+TGG + tig0016091:4884-4903 Msa1357490:CDS 55.0%
CGTGTAATACACCGGACCCT+GGG + tig0016091:4885-4904 Msa1357490:CDS 55.0%
TGCTGGATCTGTTACGCCCA+GGG - tig0016091:4904-4923 None:intergenic 55.0%
ATGCTGGATCTGTTACGCCC+AGG - tig0016091:4905-4924 None:intergenic 55.0%
GATCTGTTACGCCCAGGGTC+CGG - tig0016091:4899-4918 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0016091 gene 4350 5060 4350 ID=Msa1357490;Name=Msa1357490
tig0016091 mRNA 4350 5060 4350 ID=Msa1357490-mRNA-1;Parent=Msa1357490;Name=Msa1357490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|160
tig0016091 exon 4350 4497 4350 ID=Msa1357490-mRNA-1:exon:10408;Parent=Msa1357490-mRNA-1
tig0016091 exon 4726 5060 4726 ID=Msa1357490-mRNA-1:exon:10409;Parent=Msa1357490-mRNA-1
tig0016091 CDS 4350 4497 4350 ID=Msa1357490-mRNA-1:cds;Parent=Msa1357490-mRNA-1
tig0016091 CDS 4726 5060 4726 ID=Msa1357490-mRNA-1:cds;Parent=Msa1357490-mRNA-1
Gene Sequence

>Msa1357490

ATGGCAAGGATTGTGTTGCTGTTAATGGCCCTTTCCCTCAGCTCTATCCTCCCTTTACTTGCCACAGCTATGTTCCATATCAAAGGTTGTGTTTATTGTGATACTTGCCGTGCTGGATTCGAAACCAACGCCACATTTTACATCCAAGGTGCAAAGGTGGGAATTCGGTGCCAAGACAGGAAGACTATGGACGAAGTTTTCTACACGGAAGGAGTGACAGATTCCACTGGAACATACAACATAATGGTTGAGAATGATCACCATGACAATATTTGCAAAACTGTGCTTGTTAGCAGCCCAATATCATCGTGTAATACACCGGACCCTGGGCGTAACAGATCCAGCATTGTGCTCACTCACTACAAAAATGGTGTCCTCAACCATCTTCACTATGCAAATGCTATGGGTTACTTCAAGGATGATCCTTTGCCTCAATATTGCCACCAACTCCTCAAATATTACTTGTCTGATTCAGATGTTTGA

Protein sequence

>Msa1357490

MARIVLLLMALSLSSILPLLATAMFHIKGCVYCDTCRAGFETNATFYIQGAKVGIRCQDRKTMDEVFYTEGVTDSTGTYNIMVENDHHDNICKTVLVSSPISSCNTPDPGRNRSSIVLTHYKNGVLNHLHYANAMGYFKDDPLPQYCHQLLKYYLSDSDV*