AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1107320


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1107320 MtrunA17_Chr7g0240471 91.667 96 8 0 1 96 1 96 1.46e-60 181
Msa1107320 MtrunA17_Chr7g0240451 88.542 96 11 0 1 96 1 96 4.41e-58 174
Msa1107320 MtrunA17_Chr7g0240441 65.574 61 5 1 36 96 29 73 3.47e-18 73.2
Msa1107320 MtrunA17_Chr8g0368861 44.595 74 39 2 24 96 27 99 6.60e-12 57.8
Msa1107320 MtrunA17_Chr8g0368831 43.243 74 40 2 24 96 35 107 2.48e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1107320 AT3G53980.2 62.162 74 27 1 23 96 21 93 8.69e-28 98.6
Msa1107320 AT3G53980.1 62.162 74 27 1 23 96 21 93 8.69e-28 98.6
Msa1107320 AT5G05960.1 50.538 93 39 2 4 96 10 95 4.51e-26 94.4
Msa1107320 AT2G37870.1 41.176 85 48 2 11 94 9 92 2.38e-13 61.6

Find 26 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTGAAAACAAACATAAA+TGG 0.229535 7_3:+48876405 Msa1107320:CDS
AGCTTAAATGCTTCCATATC+TGG 0.265044 7_3:-48875797 None:intergenic
GGTATTGCAGTTCTTGTTGC+AGG 0.361788 7_3:+48875728 Msa1107320:CDS
CAACAACCTCAACTCTTTCA+AGG 0.398597 7_3:-48876385 None:intergenic
GATGTTGTTAGCTACTATTA+TGG 0.425084 7_3:+48876438 Msa1107320:exon
TGCTGAAAACAAACATAAAT+GGG 0.434655 7_3:+48876406 Msa1107320:CDS
TCAAACGTTGCTAAAATGTC+TGG 0.462464 7_3:+48875932 Msa1107320:CDS
AAGGGGTACTACACCAGATA+TGG 0.470603 7_3:+48875784 Msa1107320:CDS
GGATAGTAACAGCAATTTGA+GGG 0.472152 7_3:-48875961 None:intergenic
GGGATAGTAACAGCAATTTG+AGG 0.508534 7_3:-48875962 None:intergenic
TTTCTTGTTTGTTTATAGTG+TGG 0.514685 7_3:+48876492 Msa1107320:three_prime_UTR
GTTGAAAGTGCAGGTGAATG+TGG 0.515102 7_3:+48875761 Msa1107320:CDS
CGAGGCACAACAATTTCATA+AGG 0.539901 7_3:-48875703 None:intergenic
TCTTGTGCTGCACTTGCACA+AGG 0.541464 7_3:-48875824 None:intergenic
AAAGTGCAGGTGAATGTGGA+AGG 0.549386 7_3:+48875765 Msa1107320:CDS
ATGAAATTGTTGTGCCTCGT+TGG 0.557778 7_3:+48875707 Msa1107320:CDS
ACTCTTCCTTGAAAGAGTTG+AGG 0.566469 7_3:+48876379 Msa1107320:CDS
TGTGATCAGACTAAGAAACT+TGG 0.574065 7_3:+48875875 Msa1107320:CDS
ATTCATAGAGTTGAAAGTGC+AGG 0.578390 7_3:+48875752 Msa1107320:CDS
ATCTTTATCAAGAGTAGTAA+CGG 0.588821 7_3:-48876640 None:intergenic
AAAACTGCACAAAGACAACT+TGG 0.590774 7_3:-48875905 None:intergenic
AATACAACACAACTAAGTTG+TGG 0.625133 7_3:-48876464 None:intergenic
GTGTACACAAGTGAGCTGCT+AGG 0.629312 7_3:-48876521 None:intergenic
AGTGCAGGTGAATGTGGAAG+GGG 0.630405 7_3:+48875767 Msa1107320:CDS
AAGTGCAGGTGAATGTGGAA+GGG 0.641454 7_3:+48875766 Msa1107320:CDS
CAAGAACTGCAATACCAACG+AGG 0.744882 7_3:-48875721 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAACTTTTATATGAAAA+CGG - chr7_3:48876073-48876092 None:intergenic 10.0%
!!! TTTTAACAATAAATTTTCTT+AGG - chr7_3:48876200-48876219 None:intergenic 10.0%
!!! AAATAACTTTTATATGAAAA+CGG - chr7_3:48876073-48876092 None:intergenic 10.0%
!!! TTTTAACAATAAATTTTCTT+AGG - chr7_3:48876200-48876219 None:intergenic 10.0%
!!! GTTTTCATATAAAAGTTATT+TGG + chr7_3:48876072-48876091 Msa1107320:intron 15.0%
!! ATGTAACATTAATAATTGAT+AGG + chr7_3:48876125-48876144 Msa1107320:intron 15.0%
!!! GTTTTCATATAAAAGTTATT+TGG + chr7_3:48876072-48876091 Msa1107320:intron 15.0%
!! ATGTAACATTAATAATTGAT+AGG + chr7_3:48876125-48876144 Msa1107320:intron 15.0%
!! AATGAGATTATAGATAGTTA+AGG + chr7_3:48876151-48876170 Msa1107320:intron 20.0%
!! ATGAGATTATAGATAGTTAA+GGG + chr7_3:48876152-48876171 Msa1107320:intron 20.0%
!!! AATAAAACATACTCTTTTAG+TGG + chr7_3:48876551-48876570 Msa1107320:three_prime_UTR 20.0%
!!! AGTATTTTTAGAATTTGAGA+TGG - chr7_3:48876666-48876685 None:intergenic 20.0%
!! AATGAGATTATAGATAGTTA+AGG + chr7_3:48876151-48876170 Msa1107320:intron 20.0%
!! ATGAGATTATAGATAGTTAA+GGG + chr7_3:48876152-48876171 Msa1107320:intron 20.0%
!!! AATAAAACATACTCTTTTAG+TGG + chr7_3:48876551-48876570 Msa1107320:three_prime_UTR 20.0%
!!! AGTATTTTTAGAATTTGAGA+TGG - chr7_3:48876666-48876685 None:intergenic 20.0%
!!! TAGATAGTTAAGGGTATTAT+AGG + chr7_3:48876161-48876180 Msa1107320:intron 25.0%
! TTGTTAAAATCTTCCCTTTA+TGG + chr7_3:48876211-48876230 Msa1107320:intron 25.0%
! TTGCTGAAAACAAACATAAA+TGG + chr7_3:48876405-48876424 Msa1107320:CDS 25.0%
! TGCTGAAAACAAACATAAAT+GGG + chr7_3:48876406-48876425 Msa1107320:CDS 25.0%
!! TTTCTTGTTTGTTTATAGTG+TGG + chr7_3:48876492-48876511 Msa1107320:three_prime_UTR 25.0%
!!! AGAGTATGTTTTATTCATGA+GGG - chr7_3:48876546-48876565 None:intergenic 25.0%
!!! AAGAGTATGTTTTATTCATG+AGG - chr7_3:48876547-48876566 None:intergenic 25.0%
!! TTCCTACTAAAACATTTTAG+TGG + chr7_3:48876585-48876604 Msa1107320:three_prime_UTR 25.0%
! ATCTTTATCAAGAGTAGTAA+CGG - chr7_3:48876643-48876662 None:intergenic 25.0%
!! TGAATTTTGTTAAAGATGTG+AGG + chr7_3:48876693-48876712 Msa1107320:three_prime_UTR 25.0%
!!! TAGATAGTTAAGGGTATTAT+AGG + chr7_3:48876161-48876180 Msa1107320:intron 25.0%
! TTGTTAAAATCTTCCCTTTA+TGG + chr7_3:48876211-48876230 Msa1107320:intron 25.0%
! TTGCTGAAAACAAACATAAA+TGG + chr7_3:48876405-48876424 Msa1107320:CDS 25.0%
! TGCTGAAAACAAACATAAAT+GGG + chr7_3:48876406-48876425 Msa1107320:CDS 25.0%
!! TTTCTTGTTTGTTTATAGTG+TGG + chr7_3:48876492-48876511 Msa1107320:three_prime_UTR 25.0%
!!! AGAGTATGTTTTATTCATGA+GGG - chr7_3:48876546-48876565 None:intergenic 25.0%
!!! AAGAGTATGTTTTATTCATG+AGG - chr7_3:48876547-48876566 None:intergenic 25.0%
!! TTCCTACTAAAACATTTTAG+TGG + chr7_3:48876585-48876604 Msa1107320:three_prime_UTR 25.0%
! ATCTTTATCAAGAGTAGTAA+CGG - chr7_3:48876643-48876662 None:intergenic 25.0%
!! TGAATTTTGTTAAAGATGTG+AGG + chr7_3:48876693-48876712 Msa1107320:three_prime_UTR 25.0%
!! CTAGTTTCTAGTTCCATAAA+GGG - chr7_3:48876227-48876246 None:intergenic 30.0%
!! TCTAGTTTCTAGTTCCATAA+AGG - chr7_3:48876228-48876247 None:intergenic 30.0%
TGCCTAAGAAAAATGTGAAA+CGG + chr7_3:48876258-48876277 Msa1107320:intron 30.0%
! TACCGTTTCACATTTTTCTT+AGG - chr7_3:48876263-48876282 None:intergenic 30.0%
! CTACTTGTCAATTTTCTTGA+TGG + chr7_3:48876310-48876329 Msa1107320:intron 30.0%
GATGTTGTTAGCTACTATTA+TGG + chr7_3:48876438-48876457 Msa1107320:exon 30.0%
AATACAACACAACTAAGTTG+TGG - chr7_3:48876467-48876486 None:intergenic 30.0%
!!! GACCACTAAAATGTTTTAGT+AGG - chr7_3:48876590-48876609 None:intergenic 30.0%
!! TAAAACATTTTAGTGGTCAG+TGG + chr7_3:48876592-48876611 Msa1107320:three_prime_UTR 30.0%
!! CTAGTTTCTAGTTCCATAAA+GGG - chr7_3:48876227-48876246 None:intergenic 30.0%
!! TCTAGTTTCTAGTTCCATAA+AGG - chr7_3:48876228-48876247 None:intergenic 30.0%
TGCCTAAGAAAAATGTGAAA+CGG + chr7_3:48876258-48876277 Msa1107320:intron 30.0%
! TACCGTTTCACATTTTTCTT+AGG - chr7_3:48876263-48876282 None:intergenic 30.0%
! CTACTTGTCAATTTTCTTGA+TGG + chr7_3:48876310-48876329 Msa1107320:intron 30.0%
GATGTTGTTAGCTACTATTA+TGG + chr7_3:48876438-48876457 Msa1107320:exon 30.0%
AATACAACACAACTAAGTTG+TGG - chr7_3:48876467-48876486 None:intergenic 30.0%
!!! GACCACTAAAATGTTTTAGT+AGG - chr7_3:48876590-48876609 None:intergenic 30.0%
!! TAAAACATTTTAGTGGTCAG+TGG + chr7_3:48876592-48876611 Msa1107320:three_prime_UTR 30.0%
! ATTCATAGAGTTGAAAGTGC+AGG + chr7_3:48875752-48875771 Msa1107320:CDS 35.0%
AGCTTAAATGCTTCCATATC+TGG - chr7_3:48875800-48875819 None:intergenic 35.0%
TGTGATCAGACTAAGAAACT+TGG + chr7_3:48875875-48875894 Msa1107320:CDS 35.0%
AAAACTGCACAAAGACAACT+TGG - chr7_3:48875908-48875927 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG + chr7_3:48875932-48875951 Msa1107320:CDS 35.0%
GGATAGTAACAGCAATTTGA+GGG - chr7_3:48875964-48875983 None:intergenic 35.0%
GTCCACACTTATAACCAATA+GGG - chr7_3:48876012-48876031 None:intergenic 35.0%
TAAGTAACTAACAAGTCCCT+CGG + chr7_3:48876033-48876052 Msa1107320:intron 35.0%
! ATTCATAGAGTTGAAAGTGC+AGG + chr7_3:48875752-48875771 Msa1107320:CDS 35.0%
AGCTTAAATGCTTCCATATC+TGG - chr7_3:48875800-48875819 None:intergenic 35.0%
TGTGATCAGACTAAGAAACT+TGG + chr7_3:48875875-48875894 Msa1107320:CDS 35.0%
AAAACTGCACAAAGACAACT+TGG - chr7_3:48875908-48875927 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG + chr7_3:48875932-48875951 Msa1107320:CDS 35.0%
GGATAGTAACAGCAATTTGA+GGG - chr7_3:48875964-48875983 None:intergenic 35.0%
GTCCACACTTATAACCAATA+GGG - chr7_3:48876012-48876031 None:intergenic 35.0%
TAAGTAACTAACAAGTCCCT+CGG + chr7_3:48876033-48876052 Msa1107320:intron 35.0%
CGAGGCACAACAATTTCATA+AGG - chr7_3:48875706-48875725 None:intergenic 40.0%
ATGAAATTGTTGTGCCTCGT+TGG + chr7_3:48875707-48875726 Msa1107320:CDS 40.0%
GGGATAGTAACAGCAATTTG+AGG - chr7_3:48875965-48875984 None:intergenic 40.0%
TAGCAAAGTTGCAGCGTTTA+GGG - chr7_3:48875985-48876004 None:intergenic 40.0%
TTAGCAAAGTTGCAGCGTTT+AGG - chr7_3:48875986-48876005 None:intergenic 40.0%
AACTTTGCTAATCGCCCTAT+TGG + chr7_3:48875995-48876014 Msa1107320:intron 40.0%
CGTCCACACTTATAACCAAT+AGG - chr7_3:48876013-48876032 None:intergenic 40.0%
GGCAACTTAAAAGAAACCGA+GGG - chr7_3:48876052-48876071 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG + chr7_3:48876379-48876398 Msa1107320:CDS 40.0%
CAACAACCTCAACTCTTTCA+AGG - chr7_3:48876388-48876407 None:intergenic 40.0%
CGAGGCACAACAATTTCATA+AGG - chr7_3:48875706-48875725 None:intergenic 40.0%
ATGAAATTGTTGTGCCTCGT+TGG + chr7_3:48875707-48875726 Msa1107320:CDS 40.0%
GGGATAGTAACAGCAATTTG+AGG - chr7_3:48875965-48875984 None:intergenic 40.0%
TAGCAAAGTTGCAGCGTTTA+GGG - chr7_3:48875985-48876004 None:intergenic 40.0%
TTAGCAAAGTTGCAGCGTTT+AGG - chr7_3:48875986-48876005 None:intergenic 40.0%
AACTTTGCTAATCGCCCTAT+TGG + chr7_3:48875995-48876014 Msa1107320:intron 40.0%
CGTCCACACTTATAACCAAT+AGG - chr7_3:48876013-48876032 None:intergenic 40.0%
GGCAACTTAAAAGAAACCGA+GGG - chr7_3:48876052-48876071 None:intergenic 40.0%
ACTCTTCCTTGAAAGAGTTG+AGG + chr7_3:48876379-48876398 Msa1107320:CDS 40.0%
CAACAACCTCAACTCTTTCA+AGG - chr7_3:48876388-48876407 None:intergenic 40.0%
CAAGAACTGCAATACCAACG+AGG - chr7_3:48875724-48875743 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG + chr7_3:48875728-48875747 Msa1107320:CDS 45.0%
! GTTGAAAGTGCAGGTGAATG+TGG + chr7_3:48875761-48875780 Msa1107320:CDS 45.0%
AAAGTGCAGGTGAATGTGGA+AGG + chr7_3:48875765-48875784 Msa1107320:CDS 45.0%
AAGTGCAGGTGAATGTGGAA+GGG + chr7_3:48875766-48875785 Msa1107320:CDS 45.0%
!! AAGGGGTACTACACCAGATA+TGG + chr7_3:48875784-48875803 Msa1107320:CDS 45.0%
! CGCCCTATTGGTTATAAGTG+TGG + chr7_3:48876007-48876026 Msa1107320:intron 45.0%
CGGCAACTTAAAAGAAACCG+AGG - chr7_3:48876053-48876072 None:intergenic 45.0%
CAAGAACTGCAATACCAACG+AGG - chr7_3:48875724-48875743 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG + chr7_3:48875728-48875747 Msa1107320:CDS 45.0%
! GTTGAAAGTGCAGGTGAATG+TGG + chr7_3:48875761-48875780 Msa1107320:CDS 45.0%
AAAGTGCAGGTGAATGTGGA+AGG + chr7_3:48875765-48875784 Msa1107320:CDS 45.0%
AAGTGCAGGTGAATGTGGAA+GGG + chr7_3:48875766-48875785 Msa1107320:CDS 45.0%
!! AAGGGGTACTACACCAGATA+TGG + chr7_3:48875784-48875803 Msa1107320:CDS 45.0%
! CGCCCTATTGGTTATAAGTG+TGG + chr7_3:48876007-48876026 Msa1107320:intron 45.0%
CGGCAACTTAAAAGAAACCG+AGG - chr7_3:48876053-48876072 None:intergenic 45.0%
AGTGCAGGTGAATGTGGAAG+GGG + chr7_3:48875767-48875786 Msa1107320:CDS 50.0%
TCTTGTGCTGCACTTGCACA+AGG - chr7_3:48875827-48875846 None:intergenic 50.0%
GTGTACACAAGTGAGCTGCT+AGG - chr7_3:48876524-48876543 None:intergenic 50.0%
AGTGCAGGTGAATGTGGAAG+GGG + chr7_3:48875767-48875786 Msa1107320:CDS 50.0%
TCTTGTGCTGCACTTGCACA+AGG - chr7_3:48875827-48875846 None:intergenic 50.0%
GTGTACACAAGTGAGCTGCT+AGG - chr7_3:48876524-48876543 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 48875695 48876723 48875695 ID=Msa1107320;Name=Msa1107320
chr7_3 mRNA 48875695 48876723 48875695 ID=Msa1107320-mRNA-1;Parent=Msa1107320;Name=Msa1107320-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0|0.5|1|1|1|2|284|114
chr7_3 exon 48875695 48875973 48875695 ID=Msa1107320-mRNA-1:exon:9952;Parent=Msa1107320-mRNA-1
chr7_3 exon 48876374 48876723 48876374 ID=Msa1107320-mRNA-1:exon:9953;Parent=Msa1107320-mRNA-1
chr7_3 CDS 48875695 48875973 48875695 ID=Msa1107320-mRNA-1:cds;Parent=Msa1107320-mRNA-1
chr7_3 CDS 48876374 48876439 48876374 ID=Msa1107320-mRNA-1:cds;Parent=Msa1107320-mRNA-1
chr7_3 three_prime_UTR 48876440 48876723 48876440 ID=Msa1107320-mRNA-1:three_prime_utr;Parent=Msa1107320-mRNA-1
Gene Sequence

>Msa1107320

ATGGAGGGCCTTATGAAATTGTTGTGCCTCGTTGGTATTGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCAGGTGAATGTGGAAGGGGTACTACACCAGATATGGAAGCATTTAAGCTTGCTCCTTGTGCAAGTGCAGCACAAGATGAAAATGCTAGTGTCTCACAAAGTTGTTGTGATCAGACTAAGAAACTTGGTCAGAATCCAAGTTGTCTTTGTGCAGTTTTGCTCTCAAACGTTGCTAAAATGTCTGGTGTTAACCCTCAAATTGCTCTTACACTCTTCCTTGAAAGAGTTGAGGTTGTTGCTGAAAACAAACATAAATGGGATATGCATTGA

Protein sequence

>Msa1107320

MEGLMKLLCLVGIAVLVAGIHRVESAGECGRGTTPDMEAFKLAPCASAAQDENASVSQSCCDQTKKLGQNPSCLCAVLLSNVAKMSGVNPQIALTLFLERVEVVAENKHKWDMH*