AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1063700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1063700 MtrunA17_Chr7g0243051 92.857 224 16 0 1 224 1 224 6.38e-153 423
Msa1063700 MtrunA17_Chr4g0007601 59.009 222 71 3 1 220 1 204 8.46e-78 234
Msa1063700 MtrunA17_Chr1g0203771 42.986 221 125 1 1 220 4 224 8.45e-53 173
Msa1063700 MtrunA17_Chr6g0473621 43.836 219 122 1 3 220 18 236 2.48e-52 172
Msa1063700 MtrunA17_Chr4g0057261 42.157 204 115 2 13 216 28 228 8.96e-43 145
Msa1063700 MtrunA17_Chr2g0285591 36.946 203 126 1 20 222 31 231 1.20e-42 144
Msa1063700 MtrunA17_Chr6g0458281 42.373 118 60 2 10 123 23 136 4.52e-22 88.6
Msa1063700 MtrunA17_Chr6g0458271 42.105 114 57 2 10 117 23 133 1.36e-21 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1063700 AT3G51780.1 47.059 221 95 4 17 220 8 223 8.18e-58 184
Msa1063700 AT5G52060.1 43.137 204 114 1 19 220 39 242 6.68e-47 158
Msa1063700 AT5G07220.1 40.724 221 126 3 1 220 4 220 5.92e-44 149
Msa1063700 AT5G62100.2 36.058 208 131 1 16 223 12 217 1.72e-38 135
Msa1063700 AT5G62100.1 34.703 219 130 3 16 223 12 228 2.28e-35 127
Msa1063700 AT5G62100.5 33.679 193 126 1 10 202 6 196 3.77e-33 119
Msa1063700 AT5G62100.4 33.846 195 123 3 10 202 6 196 1.46e-32 117
Msa1063700 AT5G62100.3 34.247 146 90 3 10 153 6 147 3.22e-22 89.0
Msa1063700 AT5G62100.6 34.247 146 90 3 10 153 6 147 3.22e-22 89.0
Msa1063700 AT5G14360.1 46.939 98 48 1 20 117 49 142 9.25e-22 88.2
Msa1063700 AT5G40630.1 40.952 105 58 3 14 117 51 152 1.50e-18 79.7

Find 86 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTACATGAACCCATTTC+TGG 0.160882 7_2:+55804368 None:intergenic
CAGCAAATACTCTTCTTTAA+AGG 0.201663 7_2:-55806565 Msa1063700:CDS
GCCTTACCAATGTTGCTATA+AGG 0.247371 7_2:+55804694 None:intergenic
CAATTGGAGACCTTTGACTT+TGG 0.294069 7_2:-55804655 Msa1063700:three_prime_UTR
AAGAAGAGTATTTGCTGTTC+AGG 0.301501 7_2:+55806571 None:intergenic
GAAATCAAAACGCAAGTAAT+TGG 0.305954 7_2:+55808058 None:intergenic
TGGCTTTGAAGAATACAAAA+AGG 0.310805 7_2:-55804420 Msa1063700:three_prime_UTR
ACCGATTGGGAGATGCTTCC+CGG 0.327791 7_2:-55807971 Msa1063700:CDS
GCTGGAGCTGGATGGTATTA+AGG 0.331947 7_2:-55806170 Msa1063700:CDS
TTATAACTCTTGCCCAGAAA+TGG 0.337476 7_2:-55804381 Msa1063700:three_prime_UTR
GAACCTTGATTCTTCGTTTG+AGG 0.352388 7_2:-55804491 Msa1063700:three_prime_UTR
CCTTACCAATGTTGCTATAA+GGG 0.360694 7_2:+55804695 None:intergenic
AAACTGCTTGCACACAAAAC+TGG 0.371537 7_2:-55806601 Msa1063700:CDS
AACACCGCCGCTTATTCTTC+CGG 0.374973 7_2:-55808019 Msa1063700:CDS
GCCTTGGATGTGGCTGTCAA+TGG 0.409273 7_2:-55806253 Msa1063700:CDS
GTTGCTTATGAGGAAATTGC+TGG 0.415619 7_2:-55806188 Msa1063700:CDS
CAATCGGTATCCGCCTCATT+CGG 0.429717 7_2:+55807986 None:intergenic
CTTGAGTAACGTGTAGTAGA+TGG 0.435924 7_2:+55804586 None:intergenic
AAAGTCAAAGGTCTCCAATT+GGG 0.443694 7_2:+55804657 None:intergenic
TATAACTCTTGCCCAGAAAT+GGG 0.443890 7_2:-55804380 Msa1063700:three_prime_UTR
CAAAGTCAAAGGTCTCCAAT+TGG 0.452077 7_2:+55804656 None:intergenic
TTAAATCTTAACATGCCGTC+AGG 0.458775 7_2:+55804315 None:intergenic
AAATTCTTTGTCAGAAACCT+TGG 0.460069 7_2:+55806225 None:intergenic
AATTACATGAACCCATTTCT+GGG 0.463105 7_2:+55804369 None:intergenic
CGGTGTCTCGCCGCCGAATG+AGG 0.463239 7_2:-55807999 Msa1063700:CDS
TTACTACAGAGTTGCTTATG+AGG 0.464936 7_2:-55806198 Msa1063700:CDS
TACTACACGTTACTCAAGAT+TGG 0.466624 7_2:-55804581 Msa1063700:three_prime_UTR
GCAGGTGTTGGCCTTGGATG+TGG 0.469671 7_2:-55806263 Msa1063700:intron
GTTTGCTTGATGCTACACTA+TGG 0.477514 7_2:+55804265 None:intergenic
CTTCTTTAAAGGAAAAGAAA+AGG 0.482417 7_2:-55806554 Msa1063700:CDS
GAAGAGAGTCTCACAGAAAG+AGG 0.485676 7_2:+55807832 None:intergenic
AGGCAATATCTTTAAACACT+TGG 0.487974 7_2:-55804445 Msa1063700:three_prime_UTR
CTCATTCGGCGGCGAGACAC+CGG 0.488256 7_2:+55808000 None:intergenic
GAATAAGCGGCGGTGTTCAT+CGG 0.491708 7_2:+55808025 None:intergenic
TATGAGGAAATTGCTGGAGC+TGG 0.493825 7_2:-55806182 Msa1063700:CDS
TTGGATGTGGCTGTCAATGG+AGG 0.496098 7_2:-55806250 Msa1063700:CDS
TTTCAAGCTGTTGCTGTAGT+CGG 0.509391 7_2:-55806406 Msa1063700:CDS
CGAATGAGGCGGATACCGAT+TGG 0.510390 7_2:-55807985 Msa1063700:CDS
GAATGAGGCGGATACCGATT+GGG 0.511729 7_2:-55807984 Msa1063700:CDS
AGTCTCGGCAACAACCCAAT+TGG 0.513435 7_2:-55804671 Msa1063700:three_prime_UTR
ATATCTTTAAACACTTGGCC+TGG 0.514068 7_2:-55804440 Msa1063700:three_prime_UTR
AGGTGGACAAGCTTTGTGAC+AGG 0.514120 7_2:-55806378 Msa1063700:CDS
CAACATTGGTAAGGCAGTCT+CGG 0.520026 7_2:-55804686 Msa1063700:three_prime_UTR
GCCGGGAAGCATCTCCCAAT+CGG 0.522441 7_2:+55807970 None:intergenic
TAAATAAATTTGCCATTGTG+TGG 0.525730 7_2:-55804236 Msa1063700:three_prime_UTR
AAGTATGAGATGACGAGAGG+AGG 0.532918 7_2:-55808135 Msa1063700:five_prime_UTR
GGTCCTCAAACGAAGAATCA+AGG 0.533007 7_2:+55804488 None:intergenic
TCACAGAAAGAGGATTGAGC+TGG 0.549543 7_2:+55807842 None:intergenic
CCCTTATAGCAACATTGGTA+AGG 0.551229 7_2:-55804695 Msa1063700:exon
GGAGACCTTTGACTTTGGAA+TGG 0.552665 7_2:-55804650 Msa1063700:three_prime_UTR
GAAGAGAATTTGCACATGGA+AGG 0.561882 7_2:-55806526 Msa1063700:CDS
GATGTTGATCATCGGTCCAT+CGG 0.564771 7_2:+55807904 None:intergenic
ATCAACATCAGTGTTGCGTA+TGG 0.567834 7_2:-55807890 Msa1063700:CDS
GCCTACCCTTATAGCAACAT+TGG 0.576028 7_2:-55804700 Msa1063700:exon
TGCTGTAGTCGGATCAGAGG+TGG 0.577219 7_2:-55806395 Msa1063700:CDS
GCTTACCATTCCAAAGTCAA+AGG 0.581977 7_2:+55804645 None:intergenic
CTCTGTACGAACATACCGCC+GGG 0.592569 7_2:+55807953 None:intergenic
AGAAAGTATGAGATGACGAG+AGG 0.595466 7_2:-55808138 Msa1063700:five_prime_UTR
GGTGGACAAGCTTTGTGACA+GGG 0.600958 7_2:-55806377 Msa1063700:intron
TGGATGTGGCTGTCAATGGA+GGG 0.601202 7_2:-55806249 Msa1063700:CDS
CTGGATGGTATTAAGGCTGA+AGG 0.605506 7_2:-55806163 Msa1063700:CDS
GGTGATGATGACGATGAGGT+TGG 0.605624 7_2:+55804613 None:intergenic
CGAGACACCGGAAGAATAAG+CGG 0.607254 7_2:+55808012 None:intergenic
GGCTGTCAATGGAGGGACCA+AGG 0.612660 7_2:-55806242 Msa1063700:CDS
AGGAAATTGCTGGAGCTGGA+TGG 0.613422 7_2:-55806178 Msa1063700:CDS
GGCAAGATCAATACTCACAA+TGG 0.614241 7_2:+55804336 None:intergenic
ACGTTACTCAAGATTGGCAG+CGG 0.616842 7_2:-55804575 Msa1063700:three_prime_UTR
ACCAATGTTGCTATAAGGGT+AGG 0.621550 7_2:+55804699 None:intergenic
TCCATTGACAGCCACATCCA+AGG 0.628433 7_2:+55806252 None:intergenic
TGTTGCTGTAGTCGGATCAG+AGG 0.628867 7_2:-55806398 Msa1063700:CDS
TTTGCACATGGAAGGTGTGG+TGG 0.632918 7_2:-55806518 Msa1063700:CDS
ACGGGGAAAGACAAGAGAAG+AGG 0.635564 7_2:+55808094 None:intergenic
TGGCATAGAGAGTAATCTTG+AGG 0.638606 7_2:-55806461 Msa1063700:CDS
TGATACTTTAGATTCCCTGA+AGG 0.643562 7_2:-55804735 Msa1063700:CDS
TAAGTTGCAGAGAAAAGCTG+AGG 0.643704 7_2:-55806134 Msa1063700:intron
TCTCTGTACGAACATACCGC+CGG 0.647804 7_2:+55807952 None:intergenic
GCAACACTGATGTTGATCAT+CGG 0.648002 7_2:+55807896 None:intergenic
GAATGAAGAGAATTTGCACA+TGG 0.664413 7_2:-55806530 Msa1063700:CDS
GAATTTGCACATGGAAGGTG+TGG 0.674820 7_2:-55806521 Msa1063700:CDS
TGAGTATTGATCTTGCCTGA+CGG 0.675553 7_2:-55804330 Msa1063700:three_prime_UTR
AGTGACGATGATCATACCGA+TGG 0.677266 7_2:-55807920 Msa1063700:CDS
TAACGTGTAGTAGATGGTGG+TGG 0.679118 7_2:+55804592 None:intergenic
TGGTGGTGATGATGACGATG+AGG 0.690358 7_2:+55804609 None:intergenic
GACACCGGAAGAATAAGCGG+CGG 0.727415 7_2:+55808015 None:intergenic
GAGTAACGTGTAGTAGATGG+TGG 0.730515 7_2:+55804589 None:intergenic
GATTGGGAGATGCTTCCCGG+CGG 0.793310 7_2:-55807968 Msa1063700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAATACAAAGAAAGA+AGG - chr7_2:55804243-55804262 Msa1063700:three_prime_UTR 15.0%
!! TTAAAAATACAAAGAAAGAA+GGG - chr7_2:55804244-55804263 Msa1063700:three_prime_UTR 15.0%
!!! TATACATTCTTTTAATACAA+CGG + chr7_2:55804859-55804878 None:intergenic 15.0%
!! GAATGTATAATATGAAATTA+TGG - chr7_2:55804870-55804889 Msa1063700:intron 15.0%
!!! TATAACATATAATTTGCTTA+AGG + chr7_2:55805617-55805636 None:intergenic 15.0%
!!! TTAATTTAGTCTTTAATAAC+AGG - chr7_2:55805796-55805815 Msa1063700:intron 15.0%
!!! TAATTTAGTCTTTAATAACA+GGG - chr7_2:55805797-55805816 Msa1063700:intron 15.0%
!!! AGTTTTTAAAATGAATCTTT+CGG - chr7_2:55806839-55806858 Msa1063700:intron 15.0%
!!! ATTTGTTTTTAAAATTCCAA+TGG - chr7_2:55806987-55807006 Msa1063700:intron 15.0%
!! TAACAATTTACTTAGTTACT+TGG - chr7_2:55804772-55804791 Msa1063700:intron 20.0%
!!! AAATGTTTTTGATAGTTGAA+AGG - chr7_2:55805193-55805212 Msa1063700:intron 20.0%
!!! AATGTTTTTGATAGTTGAAA+GGG - chr7_2:55805194-55805213 Msa1063700:intron 20.0%
!!! CTTTATTTATGTATTAGTGT+AGG - chr7_2:55805495-55805514 Msa1063700:intron 20.0%
!!! AGAAAATATTTTGTTTCCAT+TGG - chr7_2:55805760-55805779 Msa1063700:intron 20.0%
!!! AATTTAGTCTTTAATAACAG+GGG - chr7_2:55805798-55805817 Msa1063700:intron 20.0%
!! AATAATAATATGGGAAACAT+GGG + chr7_2:55806083-55806102 None:intergenic 20.0%
!! TAATAATAATATGGGAAACA+TGG + chr7_2:55806084-55806103 None:intergenic 20.0%
!! CATTATAGAAACATAATAAC+TGG - chr7_2:55806109-55806128 Msa1063700:intron 20.0%
!!! ATTAACAAATTTTACTTGAG+AGG + chr7_2:55806885-55806904 None:intergenic 20.0%
!!! TATATACTTTTTTCTTGTTG+AGG - chr7_2:55806956-55806975 Msa1063700:intron 20.0%
!!! TTATTTTAGGAATGAGTTTT+CGG - chr7_2:55807048-55807067 Msa1063700:intron 20.0%
!!! AAGTTATCTAACCATAATTT+TGG - chr7_2:55807105-55807124 Msa1063700:intron 20.0%
!! AAATATCAACACCAAAATTA+TGG + chr7_2:55807119-55807138 None:intergenic 20.0%
!!! ATAATTTTGGTGTTGATATT+TGG - chr7_2:55807118-55807137 Msa1063700:intron 20.0%
!!! TTTTTTTTTTACCTTCAAAC+AGG - chr7_2:55807650-55807669 Msa1063700:intron 20.0%
!! TTAAGCAAACTACAAAAAAT+TGG + chr7_2:55807966-55807985 None:intergenic 20.0%
!!! AATTTTTTGTAGTTTGCTTA+AGG - chr7_2:55807965-55807984 Msa1063700:CDS 20.0%
!! TTCTCTCTATGTTGATATTT+TGG - chr7_2:55804618-55804637 Msa1063700:three_prime_UTR 25.0%
! TTTACTTAGTTACTTGGATT+AGG - chr7_2:55804778-55804797 Msa1063700:intron 25.0%
! TATGAAATTATGGATGAGTT+TGG - chr7_2:55804880-55804899 Msa1063700:intron 25.0%
! GTCAATATATCTAACCATTT+AGG + chr7_2:55805154-55805173 None:intergenic 25.0%
!!! ATGTTTTTGATAGTTGAAAG+GGG - chr7_2:55805195-55805214 Msa1063700:intron 25.0%
! AACTTGTAATGACAATACTA+GGG - chr7_2:55805218-55805237 Msa1063700:intron 25.0%
! AAGCTAATACATATTAGTGA+AGG - chr7_2:55805293-55805312 Msa1063700:intron 25.0%
! AAGAAAGAAAGATTGATGAA+TGG + chr7_2:55805333-55805352 None:intergenic 25.0%
! TTCTTCAACACTATTTGTTT+GGG + chr7_2:55805356-55805375 None:intergenic 25.0%
! AATTCTCGATGCAAATTAAA+TGG + chr7_2:55805466-55805485 None:intergenic 25.0%
!! TATTACTAGTTTACTCACTA+AGG + chr7_2:55805544-55805563 None:intergenic 25.0%
!! AGTTGATTAACTTGAAAACT+TGG - chr7_2:55805589-55805608 Msa1063700:intron 25.0%
!! GTTGATTAACTTGAAAACTT+GGG - chr7_2:55805590-55805609 Msa1063700:intron 25.0%
! ATCTTGATATGGAATATGTT+TGG - chr7_2:55805686-55805705 Msa1063700:intron 25.0%
! TCTTGATATGGAATATGTTT+GGG - chr7_2:55805687-55805706 Msa1063700:intron 25.0%
!! CTTCTTTAAAGGAAAAGAAA+AGG - chr7_2:55805876-55805895 Msa1063700:intron 25.0%
!!! GTTACTGATTTTAATTTTGC+AGG - chr7_2:55806149-55806168 Msa1063700:CDS 25.0%
! ATGAATTGTAAATCATCACT+TGG - chr7_2:55806435-55806454 Msa1063700:CDS 25.0%
! TCAAATACATAGATACTATC+AGG + chr7_2:55806601-55806620 None:intergenic 25.0%
!! CAAAAGAGTATATTCTTGTT+AGG - chr7_2:55806689-55806708 Msa1063700:intron 25.0%
! TCTAATCAATATTATGAGTC+AGG + chr7_2:55806761-55806780 None:intergenic 25.0%
!!! TGATATTTGGTGATGTTAAT+GGG - chr7_2:55807025-55807044 Msa1063700:intron 25.0%
!!! TGATGTTAATGGGTTATTTT+AGG - chr7_2:55807035-55807054 Msa1063700:intron 25.0%
! ACAAATAAGCTTGAAGAAAA+AGG + chr7_2:55807158-55807177 None:intergenic 25.0%
! GAACAATTCAAATTTCCTTA+CGG + chr7_2:55807222-55807241 None:intergenic 25.0%
! TAGAAAGCAACAATATAACA+TGG + chr7_2:55807272-55807291 None:intergenic 25.0%
! TTACAGGAAGAAGAAAAAAA+TGG + chr7_2:55807330-55807349 None:intergenic 25.0%
! GGTGATATTTATAATGTACT+GGG + chr7_2:55807360-55807379 None:intergenic 25.0%
! AGGTGATATTTATAATGTAC+TGG + chr7_2:55807361-55807380 None:intergenic 25.0%
! AAAAAAAAAAACCTCTGTTC+AGG + chr7_2:55807640-55807659 None:intergenic 25.0%
! AGAATCAAGGTTCAAAAATA+TGG + chr7_2:55807932-55807951 None:intergenic 25.0%
! TAAATAAATTTGCCATTGTG+TGG - chr7_2:55808194-55808213 Msa1063700:five_prime_UTR 25.0%
!! AATTGGTGCGTTTTGATAAA+CGG + chr7_2:55804358-55804377 None:intergenic 30.0%
GAAATCAAAACGCAAGTAAT+TGG + chr7_2:55804375-55804394 None:intergenic 30.0%
! GTTGAATTTTCGTTTGATCT+TGG - chr7_2:55804648-55804667 Msa1063700:three_prime_UTR 30.0%
! TTGGATTAGGTTAATGATGA+TGG - chr7_2:55804791-55804810 Msa1063700:intron 30.0%
! TGGATTAGGTTAATGATGAT+GGG - chr7_2:55804792-55804811 Msa1063700:intron 30.0%
ATGGGATTGAATATTGCATT+AGG - chr7_2:55804810-55804829 Msa1063700:intron 30.0%
TGTGATGATTGTGTTGAAAA+TGG - chr7_2:55805114-55805133 Msa1063700:intron 30.0%
!! ATATTGACTTCTTCTCTTTC+AGG - chr7_2:55805166-55805185 Msa1063700:intron 30.0%
GAACTTGTAATGACAATACT+AGG - chr7_2:55805217-55805236 Msa1063700:intron 30.0%
CTTCTTCAACACTATTTGTT+TGG + chr7_2:55805357-55805376 None:intergenic 30.0%
GAAATTTGAATTTCGAACTG+AGG - chr7_2:55805401-55805420 Msa1063700:intron 30.0%
TATTACTGTGTTACAAAGAG+TGG - chr7_2:55805435-55805454 Msa1063700:intron 30.0%
GCATCATTTGTATCTTGATA+TGG - chr7_2:55805675-55805694 Msa1063700:intron 30.0%
CTTGATATGGAATATGTTTG+GGG - chr7_2:55805688-55805707 Msa1063700:intron 30.0%
TAAGACAAAACACATACCAA+TGG + chr7_2:55805779-55805798 None:intergenic 30.0%
CAGCAAATACTCTTCTTTAA+AGG - chr7_2:55805865-55805884 Msa1063700:intron 30.0%
!!! GATTTTAATTTTGCAGGTGT+TGG - chr7_2:55806155-55806174 Msa1063700:CDS 30.0%
AAATTCTTTGTCAGAAACCT+TGG + chr7_2:55806208-55806227 None:intergenic 30.0%
AAAAGCTGAGGTAATGTTAA+TGG - chr7_2:55806308-55806327 Msa1063700:intron 30.0%
TGACCTAGCATAAAAAGTAA+TGG + chr7_2:55806371-55806390 None:intergenic 30.0%
!!! TTACTTTTTATGCTAGGTCA+TGG - chr7_2:55806371-55806390 Msa1063700:intron 30.0%
! TTGCTATGATTTTTGTGAAG+TGG - chr7_2:55806463-55806482 Msa1063700:CDS 30.0%
!!! AATATTGTTTTTCAAGCCTG+TGG - chr7_2:55806554-55806573 Msa1063700:CDS 30.0%
!!! ATTTTTTCCTAGTACTGCAA+TGG - chr7_2:55806622-55806641 Msa1063700:intron 30.0%
TTCAAAGAGATTCATGCAAT+AGG + chr7_2:55806807-55806826 None:intergenic 30.0%
AAATGAATCTTTCGGAACTT+CGG - chr7_2:55806847-55806866 Msa1063700:intron 30.0%
CTATTACTAGAAAACTCTGT+TGG - chr7_2:55806926-55806945 Msa1063700:intron 30.0%
AAAATTCCAATGGTTCTTGA+TGG - chr7_2:55806997-55807016 Msa1063700:intron 30.0%
!! GTGATATTTGGTGATGTTAA+TGG - chr7_2:55807024-55807043 Msa1063700:intron 30.0%
GTTAGATAACTTGACCTAAA+AGG + chr7_2:55807097-55807116 None:intergenic 30.0%
TGCAATTGTTTATTTCCGTA+AGG - chr7_2:55807204-55807223 Msa1063700:intron 30.0%
AGGAAGAAGAAAAAAATGGT+GGG + chr7_2:55807326-55807345 None:intergenic 30.0%
CAATGAAATCTACTTGTGTT+GGG - chr7_2:55807439-55807458 Msa1063700:intron 30.0%
! TATCCTTTTACACTGAGTTA+CGG + chr7_2:55807537-55807556 None:intergenic 30.0%
ATAAATTCTTGTACTGCTTG+CGG - chr7_2:55807554-55807573 Msa1063700:intron 30.0%
AGGCAATATCTTTAAACACT+TGG - chr7_2:55807985-55808004 Msa1063700:CDS 30.0%
! TTTTTGTATTCTTCAAAGCC+AGG + chr7_2:55808011-55808030 None:intergenic 30.0%
!! TGGCTTTGAAGAATACAAAA+AGG - chr7_2:55808010-55808029 Msa1063700:CDS 30.0%
!! GGCTTTGAAGAATACAAAAA+GGG - chr7_2:55808011-55808030 Msa1063700:CDS 30.0%
GAATACAAAAAGGGAAATGA+AGG - chr7_2:55808020-55808039 Msa1063700:CDS 30.0%
AATTACATGAACCCATTTCT+GGG + chr7_2:55808064-55808083 None:intergenic 30.0%
TAATTACATGAACCCATTTC+TGG + chr7_2:55808065-55808084 None:intergenic 30.0%
!! ATTGGTGCGTTTTGATAAAC+GGG + chr7_2:55804357-55804376 None:intergenic 35.0%
GTACATGTGAGATTGATTGT+TGG - chr7_2:55804935-55804954 Msa1063700:intron 35.0%
GATGATTGTGTTGAAAATGG+TGG - chr7_2:55805117-55805136 Msa1063700:intron 35.0%
TCGATGCAAATTAAATGGAG+GGG + chr7_2:55805461-55805480 None:intergenic 35.0%
CTCGATGCAAATTAAATGGA+GGG + chr7_2:55805462-55805481 None:intergenic 35.0%
TCTCGATGCAAATTAAATGG+AGG + chr7_2:55805463-55805482 None:intergenic 35.0%
!! AAGAAGAGTATTTGCTGTTC+AGG + chr7_2:55805862-55805881 None:intergenic 35.0%
GAATGAAGAGAATTTGCACA+TGG - chr7_2:55805900-55805919 Msa1063700:intron 35.0%
! CAAGTCAAAGCTTTTGCTTT+TGG - chr7_2:55805936-55805955 Msa1063700:intron 35.0%
TTACTACAGAGTTGCTTATG+AGG - chr7_2:55806232-55806251 Msa1063700:CDS 35.0%
!!! GGTCCATTACTTTTTATGCT+AGG - chr7_2:55806365-55806384 Msa1063700:intron 35.0%
TTATGCTAGGTCATGGAAAA+TGG - chr7_2:55806378-55806397 Msa1063700:CDS 35.0%
TGTTAAAGAGCAGCATATCA+TGG + chr7_2:55806495-55806514 None:intergenic 35.0%
! AAAGTAAAGCTTTGATCCAC+AGG + chr7_2:55806573-55806592 None:intergenic 35.0%
TAAATTGCCATTGCAGTACT+AGG + chr7_2:55806632-55806651 None:intergenic 35.0%
TGCAATGGCAATTTATATGC+TGG - chr7_2:55806637-55806656 Msa1063700:intron 35.0%
GCAATGGCAATTTATATGCT+GGG - chr7_2:55806638-55806657 Msa1063700:intron 35.0%
! CTTGATGGTCAAGTGATATT+TGG - chr7_2:55807012-55807031 Msa1063700:intron 35.0%
! ATTCCTGCTTTCAACCTTTT+AGG - chr7_2:55807080-55807099 Msa1063700:intron 35.0%
CTTGAAGAAAAAGGTAGAGA+AGG + chr7_2:55807149-55807168 None:intergenic 35.0%
CATGGCAACAATTCACAAAT+GGG + chr7_2:55807254-55807273 None:intergenic 35.0%
ACATGGCAACAATTCACAAA+TGG + chr7_2:55807255-55807274 None:intergenic 35.0%
TACATGTGTATGATGTTCTC+TGG - chr7_2:55807290-55807309 Msa1063700:intron 35.0%
CAGGAAGAAGAAAAAAATGG+TGG + chr7_2:55807327-55807346 None:intergenic 35.0%
CCAACACAAGTAGATTTCAT+TGG + chr7_2:55807441-55807460 None:intergenic 35.0%
CCAATGAAATCTACTTGTGT+TGG - chr7_2:55807438-55807457 Msa1063700:intron 35.0%
TTGATGTGTTTGTCACTATG+AGG - chr7_2:55807471-55807490 Msa1063700:intron 35.0%
TACACTGAGTTACGGAAAAT+GGG + chr7_2:55807529-55807548 None:intergenic 35.0%
TTACACTGAGTTACGGAAAA+TGG + chr7_2:55807530-55807549 None:intergenic 35.0%
TTTCCGTAACTCAGTGTAAA+AGG - chr7_2:55807531-55807550 Msa1063700:intron 35.0%
! ATCTTTAAAAGCCTGAACAG+AGG - chr7_2:55807626-55807645 Msa1063700:intron 35.0%
TGATACTTTAGATTCCCTGA+AGG - chr7_2:55807695-55807714 Msa1063700:intron 35.0%
CCTTACCAATGTTGCTATAA+GGG + chr7_2:55807738-55807757 None:intergenic 35.0%
AAAGTCAAAGGTCTCCAATT+GGG + chr7_2:55807776-55807795 None:intergenic 35.0%
TACTACACGTTACTCAAGAT+TGG - chr7_2:55807849-55807868 Msa1063700:CDS 35.0%
ATATCTTTAAACACTTGGCC+TGG - chr7_2:55807990-55808009 Msa1063700:CDS 35.0%
TTATAACTCTTGCCCAGAAA+TGG - chr7_2:55808049-55808068 Msa1063700:five_prime_UTR 35.0%
TATAACTCTTGCCCAGAAAT+GGG - chr7_2:55808050-55808069 Msa1063700:five_prime_UTR 35.0%
TTAAATCTTAACATGCCGTC+AGG + chr7_2:55808118-55808137 None:intergenic 35.0%
! AGAAAGTATGAGATGACGAG+AGG - chr7_2:55804292-55804311 Msa1063700:three_prime_UTR 40.0%
!! TTGGTGCGTTTTGATAAACG+GGG + chr7_2:55804356-55804375 None:intergenic 40.0%
GCAACACTGATGTTGATCAT+CGG + chr7_2:55804537-55804556 None:intergenic 40.0%
ATCAACATCAGTGTTGCGTA+TGG - chr7_2:55804540-55804559 Msa1063700:three_prime_UTR 40.0%
! TGATTGTTGGAGAGAGCTTT+TGG - chr7_2:55804948-55804967 Msa1063700:intron 40.0%
AACTGAAAAACAGCAGCACA+TGG + chr7_2:55804992-55805011 None:intergenic 40.0%
TGGACTTTGCTGATCCTAAA+TGG - chr7_2:55805137-55805156 Msa1063700:intron 40.0%
TTAAAGAACTTAGCTCGCTC+AGG + chr7_2:55805265-55805284 None:intergenic 40.0%
AAACTGCTTGCACACAAAAC+TGG - chr7_2:55805829-55805848 Msa1063700:intron 40.0%
GAAGAGAATTTGCACATGGA+AGG - chr7_2:55805904-55805923 Msa1063700:intron 40.0%
!! GTCAAAGCTTTTGCTTTTGG+AGG - chr7_2:55805939-55805958 Msa1063700:intron 40.0%
TGGCATAGAGAGTAATCTTG+AGG - chr7_2:55805969-55805988 Msa1063700:intron 40.0%
TTTCAAGCTGTTGCTGTAGT+CGG - chr7_2:55806024-55806043 Msa1063700:intron 40.0%
GTTGCTTATGAGGAAATTGC+TGG - chr7_2:55806242-55806261 Msa1063700:CDS 40.0%
TAAGTTGCAGAGAAAAGCTG+AGG - chr7_2:55806296-55806315 Msa1063700:intron 40.0%
TGACCTGAAAGCTTAACTGT+TGG - chr7_2:55806344-55806363 Msa1063700:intron 40.0%
!! CAAGGAAAACTGCTCTAAAG+AGG + chr7_2:55806520-55806539 None:intergenic 40.0%
! CAGTTTTCCTTGATGCACTA+TGG - chr7_2:55806528-55806547 Msa1063700:CDS 40.0%
TATTGAGCCATAGTGCATCA+AGG + chr7_2:55806538-55806557 None:intergenic 40.0%
TGGGTCAGAGAACAATAAGT+GGG - chr7_2:55806657-55806676 Msa1063700:intron 40.0%
ACTTGACCATCAAGAACCAT+TGG + chr7_2:55807006-55807025 None:intergenic 40.0%
TGACCTAAAAGGTTGAAAGC+AGG + chr7_2:55807086-55807105 None:intergenic 40.0%
! TGTACTGGGATCTAGTTTAC+AGG + chr7_2:55807346-55807365 None:intergenic 40.0%
CTTGAGATGTGTCTGATTAG+CGG - chr7_2:55807379-55807398 Msa1063700:intron 40.0%
AATCTACTTGTGTTGGGAGT+TGG - chr7_2:55807445-55807464 Msa1063700:intron 40.0%
ACCAATGTTGCTATAAGGGT+AGG + chr7_2:55807734-55807753 None:intergenic 40.0%
CCCTTATAGCAACATTGGTA+AGG - chr7_2:55807735-55807754 Msa1063700:intron 40.0%
GCCTTACCAATGTTGCTATA+AGG + chr7_2:55807739-55807758 None:intergenic 40.0%
CAAAGTCAAAGGTCTCCAAT+TGG + chr7_2:55807777-55807796 None:intergenic 40.0%
! CAATTGGAGACCTTTGACTT+TGG - chr7_2:55807775-55807794 Msa1063700:intron 40.0%
GCTTACCATTCCAAAGTCAA+AGG + chr7_2:55807788-55807807 None:intergenic 40.0%
CTTGAGTAACGTGTAGTAGA+TGG + chr7_2:55807847-55807866 None:intergenic 40.0%
GAACCTTGATTCTTCGTTTG+AGG - chr7_2:55807939-55807958 Msa1063700:CDS 40.0%
GGCAAGATCAATACTCACAA+TGG + chr7_2:55808097-55808116 None:intergenic 40.0%
!! TGAGTATTGATCTTGCCTGA+CGG - chr7_2:55808100-55808119 Msa1063700:five_prime_UTR 40.0%
GTTTGCTTGATGCTACACTA+TGG + chr7_2:55808168-55808187 None:intergenic 40.0%
AAGTATGAGATGACGAGAGG+AGG - chr7_2:55804295-55804314 Msa1063700:three_prime_UTR 45.0%
AGTGACGATGATCATACCGA+TGG - chr7_2:55804510-55804529 Msa1063700:three_prime_UTR 45.0%
GATGTTGATCATCGGTCCAT+CGG + chr7_2:55804529-55804548 None:intergenic 45.0%
TCACAGAAAGAGGATTGAGC+TGG + chr7_2:55804591-55804610 None:intergenic 45.0%
GAAGAGAGTCTCACAGAAAG+AGG + chr7_2:55804601-55804620 None:intergenic 45.0%
TTCGAACTGAGGTGAACTGT+AGG - chr7_2:55805412-55805431 Msa1063700:intron 45.0%
GAATTTGCACATGGAAGGTG+TGG - chr7_2:55805909-55805928 Msa1063700:intron 45.0%
!!! AATTTTGCAGGTGTTGGCCT+TGG - chr7_2:55806161-55806180 Msa1063700:CDS 45.0%
TATGAGGAAATTGCTGGAGC+TGG - chr7_2:55806248-55806267 Msa1063700:CDS 45.0%
CTGGATGGTATTAAGGCTGA+AGG - chr7_2:55806267-55806286 Msa1063700:intron 45.0%
GGACCAACAGTTAAGCTTTC+AGG + chr7_2:55806350-55806369 None:intergenic 45.0%
CTGGGTCAGAGAACAATAAG+TGG - chr7_2:55806656-55806675 Msa1063700:intron 45.0%
CGCTAATCAGACACATCTCA+AGG + chr7_2:55807381-55807400 None:intergenic 45.0%
AATTCTTGTACTGCTTGCGG+TGG - chr7_2:55807557-55807576 Msa1063700:intron 45.0%
!! TTGCGGTGGACTTCACATTT+TGG - chr7_2:55807571-55807590 Msa1063700:intron 45.0%
!!! GCGAGAGTTTTTTGCCTTCA+GGG + chr7_2:55807712-55807731 None:intergenic 45.0%
GCCTACCCTTATAGCAACAT+TGG - chr7_2:55807730-55807749 Msa1063700:intron 45.0%
CAACATTGGTAAGGCAGTCT+CGG - chr7_2:55807744-55807763 Msa1063700:intron 45.0%
! GGAGACCTTTGACTTTGGAA+TGG - chr7_2:55807780-55807799 Msa1063700:intron 45.0%
! TAACGTGTAGTAGATGGTGG+TGG + chr7_2:55807841-55807860 None:intergenic 45.0%
GAGTAACGTGTAGTAGATGG+TGG + chr7_2:55807844-55807863 None:intergenic 45.0%
ACGTTACTCAAGATTGGCAG+CGG - chr7_2:55807855-55807874 Msa1063700:CDS 45.0%
GGTCCTCAAACGAAGAATCA+AGG + chr7_2:55807945-55807964 None:intergenic 45.0%
!! ATGAATAATAATAATAATAT+GGG + chr7_2:55806092-55806111 None:intergenic 5.0%
!! AATGAATAATAATAATAATA+TGG + chr7_2:55806093-55806112 None:intergenic 5.0%
ACGGGGAAAGACAAGAGAAG+AGG + chr7_2:55804339-55804358 None:intergenic 50.0%
GAATAAGCGGCGGTGTTCAT+CGG + chr7_2:55804408-55804427 None:intergenic 50.0%
AACACCGCCGCTTATTCTTC+CGG - chr7_2:55804411-55804430 Msa1063700:three_prime_UTR 50.0%
CGAGACACCGGAAGAATAAG+CGG + chr7_2:55804421-55804440 None:intergenic 50.0%
CAATCGGTATCCGCCTCATT+CGG + chr7_2:55804447-55804466 None:intergenic 50.0%
! GAATGAGGCGGATACCGATT+GGG - chr7_2:55804446-55804465 Msa1063700:three_prime_UTR 50.0%
TCTCTGTACGAACATACCGC+CGG + chr7_2:55804481-55804500 None:intergenic 50.0%
! TTTGCACATGGAAGGTGTGG+TGG - chr7_2:55805912-55805931 Msa1063700:intron 50.0%
!! TTGCTTTTGGAGGATGCTGC+TGG - chr7_2:55805949-55805968 Msa1063700:intron 50.0%
TGTTGCTGTAGTCGGATCAG+AGG - chr7_2:55806032-55806051 Msa1063700:intron 50.0%
! AGGTGGACAAGCTTTGTGAC+AGG - chr7_2:55806052-55806071 Msa1063700:intron 50.0%
! GGTGGACAAGCTTTGTGACA+GGG - chr7_2:55806053-55806072 Msa1063700:intron 50.0%
! GACAAGCTTTGTGACAGGGT+GGG - chr7_2:55806057-55806076 Msa1063700:intron 50.0%
TCCATTGACAGCCACATCCA+AGG + chr7_2:55806181-55806200 None:intergenic 50.0%
TTGGATGTGGCTGTCAATGG+AGG - chr7_2:55806180-55806199 Msa1063700:CDS 50.0%
TGGATGTGGCTGTCAATGGA+GGG - chr7_2:55806181-55806200 Msa1063700:CDS 50.0%
AGGAAATTGCTGGAGCTGGA+TGG - chr7_2:55806252-55806271 Msa1063700:CDS 50.0%
GCTGGAGCTGGATGGTATTA+AGG - chr7_2:55806260-55806279 Msa1063700:CDS 50.0%
TACACGACGCACCTGTTTGA+AGG + chr7_2:55807664-55807683 None:intergenic 50.0%
!!! GGCGAGAGTTTTTTGCCTTC+AGG + chr7_2:55807713-55807732 None:intergenic 50.0%
AGTCTCGGCAACAACCCAAT+TGG - chr7_2:55807759-55807778 Msa1063700:intron 50.0%
! GGTGATGATGACGATGAGGT+TGG + chr7_2:55807820-55807839 None:intergenic 50.0%
! TGGTGGTGATGATGACGATG+AGG + chr7_2:55807824-55807843 None:intergenic 50.0%
GACACCGGAAGAATAAGCGG+CGG + chr7_2:55804418-55804437 None:intergenic 55.0%
CGAATGAGGCGGATACCGAT+TGG - chr7_2:55804445-55804464 Msa1063700:three_prime_UTR 55.0%
ACCGATTGGGAGATGCTTCC+CGG - chr7_2:55804459-55804478 Msa1063700:three_prime_UTR 55.0%
CTCTGTACGAACATACCGCC+GGG + chr7_2:55804480-55804499 None:intergenic 55.0%
TGCTGTAGTCGGATCAGAGG+TGG - chr7_2:55806035-55806054 Msa1063700:intron 55.0%
! GGACAAGCTTTGTGACAGGG+TGG - chr7_2:55806056-55806075 Msa1063700:intron 55.0%
GCCTTGGATGTGGCTGTCAA+TGG - chr7_2:55806177-55806196 Msa1063700:CDS 55.0%
TCGGTATCCGCCTCATTCGG+CGG + chr7_2:55804444-55804463 None:intergenic 60.0%
!! GCCGGGAAGCATCTCCCAAT+CGG + chr7_2:55804463-55804482 None:intergenic 60.0%
GATTGGGAGATGCTTCCCGG+CGG - chr7_2:55804462-55804481 Msa1063700:three_prime_UTR 60.0%
!! GCAGGTGTTGGCCTTGGATG+TGG - chr7_2:55806167-55806186 Msa1063700:CDS 60.0%
GGCTGTCAATGGAGGGACCA+AGG - chr7_2:55806188-55806207 Msa1063700:CDS 60.0%
CTCATTCGGCGGCGAGACAC+CGG + chr7_2:55804433-55804452 None:intergenic 65.0%
TGTCTCGCCGCCGAATGAGG+CGG - chr7_2:55804434-55804453 Msa1063700:three_prime_UTR 65.0%
CGGTGTCTCGCCGCCGAATG+AGG - chr7_2:55804431-55804450 Msa1063700:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 55804232 55808220 55804232 ID=Msa1063700;Name=Msa1063700
chr7_2 mRNA 55804232 55808220 55804232 ID=Msa1063700-mRNA-1;Parent=Msa1063700;Name=Msa1063700-mRNA-1;_AED=0.19;_eAED=0.19;_QI=176|1|1|1|1|1|4|477|224
chr7_2 exon 55804232 55804780 55804232 ID=Msa1063700-mRNA-1:exon:10490;Parent=Msa1063700-mRNA-1
chr7_2 exon 55806135 55806281 55806135 ID=Msa1063700-mRNA-1:exon:10489;Parent=Msa1063700-mRNA-1
chr7_2 exon 55806378 55806634 55806378 ID=Msa1063700-mRNA-1:exon:10488;Parent=Msa1063700-mRNA-1
chr7_2 exon 55807846 55808220 55807846 ID=Msa1063700-mRNA-1:exon:10487;Parent=Msa1063700-mRNA-1
chr7_2 five_prime_UTR 55808045 55808220 55808045 ID=Msa1063700-mRNA-1:five_prime_utr;Parent=Msa1063700-mRNA-1
chr7_2 CDS 55807846 55808044 55807846 ID=Msa1063700-mRNA-1:cds;Parent=Msa1063700-mRNA-1
chr7_2 CDS 55806378 55806634 55806378 ID=Msa1063700-mRNA-1:cds;Parent=Msa1063700-mRNA-1
chr7_2 CDS 55806135 55806281 55806135 ID=Msa1063700-mRNA-1:cds;Parent=Msa1063700-mRNA-1
chr7_2 CDS 55804709 55804780 55804709 ID=Msa1063700-mRNA-1:cds;Parent=Msa1063700-mRNA-1
chr7_2 three_prime_UTR 55804232 55804708 55804232 ID=Msa1063700-mRNA-1:three_prime_utr;Parent=Msa1063700-mRNA-1
Gene Sequence

>Msa1063700

ATGAACACCGCCGCTTATTCTTCCGGTGTCTCGCCGCCGAATGAGGCGGATACCGATTGGGAGATGCTTCCCGGCGGTATGTTCGTACAGAGACGCGAATCAAGTGACGATGATCATACCGATGGACCGATGATCAACATCAGTGTTGCGTATGGTTCTTCTAATCACGAAGTTTATCTTCCAGCTCAATCCTCTTTCTGGGATGTTAAGAAACTGCTTGCACACAAAACTGGTTTAAAGCCTGAACAGCAAATACTCTTCTTTAAAGGAAAAGAAAAGGAGAATGAAGAGAATTTGCACATGGAAGGTGTGGTGGACAAGTCAAAGCTTTTGCTTTTGGAGGATGCTGCTGGCATAGAGAGTAATCTTGAGGAAGTTAGAAAACAAAATGAAATGTTAAAAGCTTTTCAAGCTGTTGCTGTAGTCGGATCAGAGGTGGACAAGCTTTGTGACAGGGTGTTGGCCTTGGATGTGGCTGTCAATGGAGGGACCAAGGTTTCTGACAAAGAATTTGTTGTTACTACAGAGTTGCTTATGAGGAAATTGCTGGAGCTGGATGGTATTAAGGCTGAAGGTGAAGCTAAGTTGCAGAGAAAAGCTGAGGTGCGTCGTGTACAGAACGCTGTTGATACTTTAGATTCCCTGAAGGCAAAAAACTCTCGCCTACCCTTATAG
Protein sequence

>Msa1063700

MNTAAYSSGVSPPNEADTDWEMLPGGMFVQRRESSDDDHTDGPMINISVAYGSSNHEVYLPAQSSFWDVKKLLAHKTGLKPEQQILFFKGKEKENEENLHMEGVVDKSKLLLLEDAAGIESNLEEVRKQNEMLKAFQAVAVVGSEVDKLCDRVLALDVAVNGGTKVSDKEFVVTTELLMRKLLELDGIKAEGEAKLQRKAEVRRVQNAVDTLDSLKAKNSRLPL*