AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1115640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1115640 MtrunA17_Chr7g0247071 93.662 142 6 1 1 139 1 142 3.60e-93 265
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1115640 AT4G00525.1 42.636 129 68 2 16 139 13 140 4.06e-26 96.3
Msa1115640 AT1G01715.1 33.333 123 75 2 19 137 18 137 1.66e-17 73.9
Msa1115640 AT1G01715.2 33.333 123 75 2 19 137 18 137 1.66e-17 73.9

Find 40 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGGGTTTCCGATATTTC+TGG 0.144913 7_3:+63116678 None:intergenic
TTTGCAAAGAATGTAAATTT+TGG 0.155008 7_3:-63114610 Msa1115640:three_prime_UTR
CTCACGAAGCATTCGTTATT+TGG 0.162234 7_3:-63116809 None:intergenic
CGGAAACCCATGAAGCATTT+AGG 0.280043 7_3:-63116668 Msa1115640:CDS
AACTATATAATCTGAAGTTT+TGG 0.282198 7_3:-63114434 None:intergenic
GTTGAGGAAGAAAGGGTCTT+TGG 0.286323 7_3:+63116520 None:intergenic
TTTCTTCCTCAACTCAATTC+AGG 0.313488 7_3:-63116510 Msa1115640:intron
CGGAGCTCCTAAATGCTTCA+TGG 0.344784 7_3:+63116661 None:intergenic
TTGAGGAAGAAAGGGTCTTT+GGG 0.356301 7_3:+63116521 None:intergenic
TGAATTTCGAACAACTCTTC+CGG 0.369290 7_3:-63116580 Msa1115640:CDS
AGAGTATTTGAATCTGAATA+TGG 0.376331 7_3:-63116703 Msa1115640:exon
GCTCAAATCCACTCTCCTTT+CGG 0.398015 7_3:-63116553 Msa1115640:CDS
TTTACTTTCTCAGCACTCTC+TGG 0.400723 7_3:+63114871 None:intergenic
GAATATGGCTCCAGAAATAT+CGG 0.404108 7_3:-63116688 Msa1115640:exon
ATTTAGGAGCTCCGAAGATT+CGG 0.425015 7_3:-63116652 Msa1115640:CDS
CTCCTCTGAATCCGAATCTT+CGG 0.426583 7_3:+63116641 None:intergenic
GGAGTTGGAGTCTGATTTGA+AGG 0.453695 7_3:-63116622 Msa1115640:CDS
TGAATTGAGTTGAGGAAGAA+AGG 0.454206 7_3:+63116512 None:intergenic
AGAGTGGATTTGAGCTGATC+CGG 0.464922 7_3:+63116561 None:intergenic
ACATACATCAAGGCTTCAAA+AGG 0.498998 7_3:-63114738 Msa1115640:CDS
AGATTCGGATTCAGAGGAGT+TGG 0.509094 7_3:-63116637 Msa1115640:CDS
GGAGCTCCTAAATGCTTCAT+GGG 0.515033 7_3:+63116662 None:intergenic
GGCTTCAAAAGGAAGAAGAC+TGG 0.521999 7_3:-63114727 Msa1115640:CDS
GAACAAAACATTTCTAGAGA+GGG 0.525493 7_3:-63114916 Msa1115640:CDS
AGAAGACTGGATAAAGACTA+AGG 0.530427 7_3:-63114714 Msa1115640:exon
GAATTGAGTTGAGGAAGAAA+GGG 0.533817 7_3:+63116513 None:intergenic
TGAGAAAGTAAAATTACTAA+AGG 0.538575 7_3:-63114860 Msa1115640:CDS
ACGAAGCATTCGTTATTTGG+TGG 0.538639 7_3:-63116806 None:intergenic
AGAACAAAACATTTCTAGAG+AGG 0.555444 7_3:-63114917 Msa1115640:CDS
CTCCGAAGATTCGGATTCAG+AGG 0.569262 7_3:-63116643 Msa1115640:CDS
TTCAAGAACCGAAAGGAGAG+TGG 0.588319 7_3:+63116545 None:intergenic
AATGCTGCAAACATACATCA+AGG 0.591855 7_3:-63114748 Msa1115640:CDS
TGAAATTTAATCAGCTACCA+TGG 0.593317 7_3:+63114502 None:intergenic
CTTGCCAGTGTAGAATTGTA+CGG 0.593575 7_3:-63114689 Msa1115640:three_prime_UTR
AGTATGAACCACATGGTCCA+TGG 0.603428 7_3:-63114519 Msa1115640:three_prime_UTR
ATCAGCTACCATGGACCATG+TGG 0.640288 7_3:+63114511 None:intergenic
TTTGGGATTCAAGAACCGAA+AGG 0.657974 7_3:+63116538 None:intergenic
TTTGCCGTACAATTCTACAC+TGG 0.666689 7_3:+63114685 None:intergenic
AATGTACCTGAATTGAGTTG+AGG 0.675250 7_3:+63116504 None:intergenic
TATGTCTAGTATGAACCACA+TGG 0.725584 7_3:-63114526 Msa1115640:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAATACTAAATATA+TGG + chr7_3:63116245-63116264 None:intergenic 10.0%
!! GAAAAAAAATACTAAATATA+TGG + chr7_3:63116245-63116264 None:intergenic 10.0%
!!! GAAGAAAAAAAATCAAATTT+TGG + chr7_3:63114801-63114820 None:intergenic 15.0%
!!! ATATTATTTTTATTTTGTGC+CGG - chr7_3:63115772-63115791 Msa1115640:intron 15.0%
!!! TTTTATGTAAAGATTACAAA+TGG - chr7_3:63115857-63115876 Msa1115640:intron 15.0%
!!! GAAGAAAAAAAATCAAATTT+TGG + chr7_3:63114801-63114820 None:intergenic 15.0%
!!! ATATTATTTTTATTTTGTGC+CGG - chr7_3:63115772-63115791 Msa1115640:intron 15.0%
!!! TTTTATGTAAAGATTACAAA+TGG - chr7_3:63115857-63115876 Msa1115640:intron 15.0%
!! AAAACCCTAGAAAATAAAAA+GGG + chr7_3:63114757-63114776 None:intergenic 20.0%
!!! GATTTTTTTTCTTCGTATTT+TGG - chr7_3:63114807-63114826 Msa1115640:CDS 20.0%
!! AATTCCAAACATCAATTATT+GGG + chr7_3:63114879-63114898 None:intergenic 20.0%
!! AAATTCCAAACATCAATTAT+TGG + chr7_3:63114880-63114899 None:intergenic 20.0%
!! AAGTTTATGGAAAATTTAAC+TGG - chr7_3:63115061-63115080 Msa1115640:intron 20.0%
!!! TAAAAGTACAACCAATATAT+AGG + chr7_3:63115256-63115275 None:intergenic 20.0%
!! TAAATACTAGAGTAGATTTA+TGG + chr7_3:63115418-63115437 None:intergenic 20.0%
!!! TCATAGGTATTATTATTTTC+TGG - chr7_3:63115637-63115656 Msa1115640:intron 20.0%
!!! TCTACATATCAAATACATTT+TGG + chr7_3:63116267-63116286 None:intergenic 20.0%
!! TGAGAAAGTAAAATTACTAA+AGG - chr7_3:63116371-63116390 Msa1115640:intron 20.0%
!!! TTTGCAAAGAATGTAAATTT+TGG - chr7_3:63116621-63116640 Msa1115640:CDS 20.0%
!! AAAACCCTAGAAAATAAAAA+GGG + chr7_3:63114757-63114776 None:intergenic 20.0%
!!! GATTTTTTTTCTTCGTATTT+TGG - chr7_3:63114807-63114826 Msa1115640:CDS 20.0%
!! AATTCCAAACATCAATTATT+GGG + chr7_3:63114879-63114898 None:intergenic 20.0%
!! AAATTCCAAACATCAATTAT+TGG + chr7_3:63114880-63114899 None:intergenic 20.0%
!! AAGTTTATGGAAAATTTAAC+TGG - chr7_3:63115061-63115080 Msa1115640:intron 20.0%
!!! TAAAAGTACAACCAATATAT+AGG + chr7_3:63115256-63115275 None:intergenic 20.0%
!! TAAATACTAGAGTAGATTTA+TGG + chr7_3:63115418-63115437 None:intergenic 20.0%
!!! TCATAGGTATTATTATTTTC+TGG - chr7_3:63115637-63115656 Msa1115640:intron 20.0%
!!! TCTACATATCAAATACATTT+TGG + chr7_3:63116267-63116286 None:intergenic 20.0%
!! TGAGAAAGTAAAATTACTAA+AGG - chr7_3:63116371-63116390 Msa1115640:intron 20.0%
!!! TTTGCAAAGAATGTAAATTT+TGG - chr7_3:63116621-63116640 Msa1115640:CDS 20.0%
!! AGAGTATTTGAATCTGAATA+TGG - chr7_3:63114528-63114547 Msa1115640:three_prime_UTR 25.0%
!!! TACTTCCCTTTTTATTTTCT+AGG - chr7_3:63114749-63114768 Msa1115640:CDS 25.0%
!!! ACTTCCCTTTTTATTTTCTA+GGG - chr7_3:63114750-63114769 Msa1115640:CDS 25.0%
! GAAAACCCTAGAAAATAAAA+AGG + chr7_3:63114758-63114777 None:intergenic 25.0%
!!! ACTGATTTTTTGCATTTAAC+TGG - chr7_3:63114849-63114868 Msa1115640:CDS 25.0%
!!! CTGATTTTTTGCATTTAACT+GGG - chr7_3:63114850-63114869 Msa1115640:CDS 25.0%
!!! AGTTTGATGATTTCTGTTTT+TGG - chr7_3:63114912-63114931 Msa1115640:CDS 25.0%
!!! GTTTGATGATTTCTGTTTTT+GGG - chr7_3:63114913-63114932 Msa1115640:CDS 25.0%
! TTTACGAGATTCTAAGTTTA+TGG - chr7_3:63115048-63115067 Msa1115640:intron 25.0%
! AACAATCAGAGAAATAAACA+TGG + chr7_3:63115219-63115238 None:intergenic 25.0%
! ACTAAGAATATTATCATGCA+TGG - chr7_3:63115276-63115295 Msa1115640:intron 25.0%
!! AATACATGGTTTTCTATGAA+AGG + chr7_3:63115461-63115480 None:intergenic 25.0%
!!! ATCATGAGGTATTTTATCAT+AGG - chr7_3:63115621-63115640 Msa1115640:intron 25.0%
!!! TGCACTTTAAAATCAGTAAT+GGG + chr7_3:63115707-63115726 None:intergenic 25.0%
!!! ATGCACTTTAAAATCAGTAA+TGG + chr7_3:63115708-63115727 None:intergenic 25.0%
! GCAGATGATCAAAATATTTA+GGG + chr7_3:63115817-63115836 None:intergenic 25.0%
! TGCAGATGATCAAAATATTT+AGG + chr7_3:63115818-63115837 None:intergenic 25.0%
!!! TTGTTATTTGAATGATCTGT+TGG - chr7_3:63115977-63115996 Msa1115640:intron 25.0%
! ATCTTGAGAGAGTTAAATAA+AGG + chr7_3:63116221-63116240 None:intergenic 25.0%
!! TTCATCCTTTTCAAAACTAT+TGG + chr7_3:63116426-63116445 None:intergenic 25.0%
!! ATTTTGGATAATACAATGTG+TGG - chr7_3:63116637-63116656 Msa1115640:CDS 25.0%
!! AGAGTATTTGAATCTGAATA+TGG - chr7_3:63114528-63114547 Msa1115640:three_prime_UTR 25.0%
!!! TACTTCCCTTTTTATTTTCT+AGG - chr7_3:63114749-63114768 Msa1115640:CDS 25.0%
!!! ACTTCCCTTTTTATTTTCTA+GGG - chr7_3:63114750-63114769 Msa1115640:CDS 25.0%
! GAAAACCCTAGAAAATAAAA+AGG + chr7_3:63114758-63114777 None:intergenic 25.0%
!!! ACTGATTTTTTGCATTTAAC+TGG - chr7_3:63114849-63114868 Msa1115640:CDS 25.0%
!!! CTGATTTTTTGCATTTAACT+GGG - chr7_3:63114850-63114869 Msa1115640:CDS 25.0%
!!! AGTTTGATGATTTCTGTTTT+TGG - chr7_3:63114912-63114931 Msa1115640:CDS 25.0%
!!! GTTTGATGATTTCTGTTTTT+GGG - chr7_3:63114913-63114932 Msa1115640:CDS 25.0%
! TTTACGAGATTCTAAGTTTA+TGG - chr7_3:63115048-63115067 Msa1115640:intron 25.0%
! AACAATCAGAGAAATAAACA+TGG + chr7_3:63115219-63115238 None:intergenic 25.0%
! ACTAAGAATATTATCATGCA+TGG - chr7_3:63115276-63115295 Msa1115640:intron 25.0%
!! AATACATGGTTTTCTATGAA+AGG + chr7_3:63115461-63115480 None:intergenic 25.0%
!!! ATCATGAGGTATTTTATCAT+AGG - chr7_3:63115621-63115640 Msa1115640:intron 25.0%
!!! TGCACTTTAAAATCAGTAAT+GGG + chr7_3:63115707-63115726 None:intergenic 25.0%
!!! ATGCACTTTAAAATCAGTAA+TGG + chr7_3:63115708-63115727 None:intergenic 25.0%
! GCAGATGATCAAAATATTTA+GGG + chr7_3:63115817-63115836 None:intergenic 25.0%
! TGCAGATGATCAAAATATTT+AGG + chr7_3:63115818-63115837 None:intergenic 25.0%
!!! TTGTTATTTGAATGATCTGT+TGG - chr7_3:63115977-63115996 Msa1115640:intron 25.0%
! ATCTTGAGAGAGTTAAATAA+AGG + chr7_3:63116221-63116240 None:intergenic 25.0%
!! TTCATCCTTTTCAAAACTAT+TGG + chr7_3:63116426-63116445 None:intergenic 25.0%
!! ATTTTGGATAATACAATGTG+TGG - chr7_3:63116637-63116656 Msa1115640:CDS 25.0%
!!! ATTTTGGTTGACCTATGAAA+GGG + chr7_3:63114785-63114804 None:intergenic 30.0%
!!! AATTTTGGTTGACCTATGAA+AGG + chr7_3:63114786-63114805 None:intergenic 30.0%
AAACAGAAATCATCAAACTC+AGG + chr7_3:63114911-63114930 None:intergenic 30.0%
CAAAAACAAGTAAACTGTCA+CGG + chr7_3:63114939-63114958 None:intergenic 30.0%
TATGCTTCATCGTATTGAAA+CGG + chr7_3:63114984-63115003 None:intergenic 30.0%
AATACGATGAAGCATATACT+TGG - chr7_3:63114988-63115007 Msa1115640:intron 30.0%
! TGTTTGAGATTGCTTAAATC+TGG - chr7_3:63115120-63115139 Msa1115640:intron 30.0%
AAAGCCATTACAATAAAGCA+TGG + chr7_3:63115177-63115196 None:intergenic 30.0%
! ATGCATGGATTTGTTCAATT+TGG - chr7_3:63115291-63115310 Msa1115640:intron 30.0%
! TGGATTTGTTCAATTTGGTT+TGG - chr7_3:63115296-63115315 Msa1115640:intron 30.0%
TCTATGAAAGGTTCAAACAA+TGG + chr7_3:63115449-63115468 None:intergenic 30.0%
TTAGGATCTAATGGAATACA+TGG + chr7_3:63115475-63115494 None:intergenic 30.0%
! CATCTGTGAATTTGATGATT+CGG - chr7_3:63115504-63115523 Msa1115640:intron 30.0%
!!! TATTTTGATCATCTGCATGT+TGG - chr7_3:63115821-63115840 Msa1115640:intron 30.0%
TAGCATGCAATATCCATATT+TGG + chr7_3:63116116-63116135 None:intergenic 30.0%
! GACCTTATTTACATTGTTAC+AGG - chr7_3:63116285-63116304 Msa1115640:intron 30.0%
CACCTGTAACAATGTAAATA+AGG + chr7_3:63116290-63116309 None:intergenic 30.0%
! AGAACAAAACATTTCTAGAG+AGG - chr7_3:63116314-63116333 Msa1115640:intron 30.0%
! GAACAAAACATTTCTAGAGA+GGG - chr7_3:63116315-63116334 Msa1115640:intron 30.0%
!!! CTATGCCAATAGTTTTGAAA+AGG - chr7_3:63116418-63116437 Msa1115640:intron 30.0%
!!! AAAGCGAAATTGTTTTGAAG+AGG - chr7_3:63116582-63116601 Msa1115640:CDS 30.0%
TGAAATTTAATCAGCTACCA+TGG + chr7_3:63116732-63116751 None:intergenic 30.0%
!!! ATTTTGGTTGACCTATGAAA+GGG + chr7_3:63114785-63114804 None:intergenic 30.0%
!!! AATTTTGGTTGACCTATGAA+AGG + chr7_3:63114786-63114805 None:intergenic 30.0%
AAACAGAAATCATCAAACTC+AGG + chr7_3:63114911-63114930 None:intergenic 30.0%
CAAAAACAAGTAAACTGTCA+CGG + chr7_3:63114939-63114958 None:intergenic 30.0%
TATGCTTCATCGTATTGAAA+CGG + chr7_3:63114984-63115003 None:intergenic 30.0%
AATACGATGAAGCATATACT+TGG - chr7_3:63114988-63115007 Msa1115640:intron 30.0%
! TGTTTGAGATTGCTTAAATC+TGG - chr7_3:63115120-63115139 Msa1115640:intron 30.0%
AAAGCCATTACAATAAAGCA+TGG + chr7_3:63115177-63115196 None:intergenic 30.0%
! ATGCATGGATTTGTTCAATT+TGG - chr7_3:63115291-63115310 Msa1115640:intron 30.0%
! TGGATTTGTTCAATTTGGTT+TGG - chr7_3:63115296-63115315 Msa1115640:intron 30.0%
TCTATGAAAGGTTCAAACAA+TGG + chr7_3:63115449-63115468 None:intergenic 30.0%
TTAGGATCTAATGGAATACA+TGG + chr7_3:63115475-63115494 None:intergenic 30.0%
! CATCTGTGAATTTGATGATT+CGG - chr7_3:63115504-63115523 Msa1115640:intron 30.0%
!!! TATTTTGATCATCTGCATGT+TGG - chr7_3:63115821-63115840 Msa1115640:intron 30.0%
TAGCATGCAATATCCATATT+TGG + chr7_3:63116116-63116135 None:intergenic 30.0%
! GACCTTATTTACATTGTTAC+AGG - chr7_3:63116285-63116304 Msa1115640:intron 30.0%
CACCTGTAACAATGTAAATA+AGG + chr7_3:63116290-63116309 None:intergenic 30.0%
! AGAACAAAACATTTCTAGAG+AGG - chr7_3:63116314-63116333 Msa1115640:intron 30.0%
! GAACAAAACATTTCTAGAGA+GGG - chr7_3:63116315-63116334 Msa1115640:intron 30.0%
!!! CTATGCCAATAGTTTTGAAA+AGG - chr7_3:63116418-63116437 Msa1115640:intron 30.0%
!!! AAAGCGAAATTGTTTTGAAG+AGG - chr7_3:63116582-63116601 Msa1115640:CDS 30.0%
TGAAATTTAATCAGCTACCA+TGG + chr7_3:63116732-63116751 None:intergenic 30.0%
GAATATGGCTCCAGAAATAT+CGG - chr7_3:63114543-63114562 Msa1115640:three_prime_UTR 35.0%
TGAATTTCGAACAACTCTTC+CGG - chr7_3:63114651-63114670 Msa1115640:three_prime_UTR 35.0%
!! GAATTGAGTTGAGGAAGAAA+GGG + chr7_3:63114721-63114740 None:intergenic 35.0%
!! TGAATTGAGTTGAGGAAGAA+AGG + chr7_3:63114722-63114741 None:intergenic 35.0%
TTTCTTCCTCAACTCAATTC+AGG - chr7_3:63114721-63114740 Msa1115640:exon 35.0%
! AATGTACCTGAATTGAGTTG+AGG + chr7_3:63114730-63114749 None:intergenic 35.0%
! GGTTTTCTTTTCCCTTTCAT+AGG - chr7_3:63114771-63114790 Msa1115640:CDS 35.0%
GTGACCCAATAATTGATGTT+TGG - chr7_3:63114872-63114891 Msa1115640:CDS 35.0%
!! CGTGACAGTTTACTTGTTTT+TGG - chr7_3:63114937-63114956 Msa1115640:intron 35.0%
GATTGCTTAAATCTGGTTGT+TGG - chr7_3:63115127-63115146 Msa1115640:intron 35.0%
! GTGACCATGCTTTATTGTAA+TGG - chr7_3:63115170-63115189 Msa1115640:intron 35.0%
! TCTCTGATTGTTGTGAGTTT+TGG - chr7_3:63115227-63115246 Msa1115640:intron 35.0%
!!! AGTTTTGGAGGCCTATATAT+TGG - chr7_3:63115242-63115261 Msa1115640:intron 35.0%
TCCATTAGATCCTAATGAAC+TGG - chr7_3:63115480-63115499 Msa1115640:intron 35.0%
TCCAGTTCATTAGGATCTAA+TGG + chr7_3:63115484-63115503 None:intergenic 35.0%
! AATGAATAGGGTACTTTGGT+TGG + chr7_3:63115555-63115574 None:intergenic 35.0%
! GATGAATGAATAGGGTACTT+TGG + chr7_3:63115559-63115578 None:intergenic 35.0%
GTGTCTCTAAATTGATCATG+AGG - chr7_3:63115607-63115626 Msa1115640:intron 35.0%
ATCGACTAGAGAATTATCAG+TGG - chr7_3:63115669-63115688 Msa1115640:intron 35.0%
!!! TTTTATTTTGTGCCGGTGTT+AGG - chr7_3:63115779-63115798 Msa1115640:intron 35.0%
ATTTGCAGAAAACCTAACAC+CGG + chr7_3:63115794-63115813 None:intergenic 35.0%
TTCTACATCTGCTTATTTGC+AGG - chr7_3:63115897-63115916 Msa1115640:intron 35.0%
TCTACATCTGCTTATTTGCA+GGG - chr7_3:63115898-63115917 Msa1115640:intron 35.0%
!! AAAATGCAGGGTTAGTTTCT+AGG + chr7_3:63116069-63116088 None:intergenic 35.0%
CTACTATGCGTAACCAAATA+TGG - chr7_3:63116100-63116119 Msa1115640:intron 35.0%
! AGAAATGATCTTGTGCCATT+TGG - chr7_3:63116168-63116187 Msa1115640:intron 35.0%
AATGCTGCAAACATACATCA+AGG - chr7_3:63116483-63116502 Msa1115640:intron 35.0%
ACATACATCAAGGCTTCAAA+AGG - chr7_3:63116493-63116512 Msa1115640:intron 35.0%
AGAAGACTGGATAAAGACTA+AGG - chr7_3:63116517-63116536 Msa1115640:CDS 35.0%
TATGTCTAGTATGAACCACA+TGG - chr7_3:63116705-63116724 Msa1115640:exon 35.0%
GAATATGGCTCCAGAAATAT+CGG - chr7_3:63114543-63114562 Msa1115640:three_prime_UTR 35.0%
TGAATTTCGAACAACTCTTC+CGG - chr7_3:63114651-63114670 Msa1115640:three_prime_UTR 35.0%
!! GAATTGAGTTGAGGAAGAAA+GGG + chr7_3:63114721-63114740 None:intergenic 35.0%
!! TGAATTGAGTTGAGGAAGAA+AGG + chr7_3:63114722-63114741 None:intergenic 35.0%
TTTCTTCCTCAACTCAATTC+AGG - chr7_3:63114721-63114740 Msa1115640:exon 35.0%
! AATGTACCTGAATTGAGTTG+AGG + chr7_3:63114730-63114749 None:intergenic 35.0%
! GGTTTTCTTTTCCCTTTCAT+AGG - chr7_3:63114771-63114790 Msa1115640:CDS 35.0%
GTGACCCAATAATTGATGTT+TGG - chr7_3:63114872-63114891 Msa1115640:CDS 35.0%
!! CGTGACAGTTTACTTGTTTT+TGG - chr7_3:63114937-63114956 Msa1115640:intron 35.0%
GATTGCTTAAATCTGGTTGT+TGG - chr7_3:63115127-63115146 Msa1115640:intron 35.0%
! GTGACCATGCTTTATTGTAA+TGG - chr7_3:63115170-63115189 Msa1115640:intron 35.0%
! TCTCTGATTGTTGTGAGTTT+TGG - chr7_3:63115227-63115246 Msa1115640:intron 35.0%
!!! AGTTTTGGAGGCCTATATAT+TGG - chr7_3:63115242-63115261 Msa1115640:intron 35.0%
TCCATTAGATCCTAATGAAC+TGG - chr7_3:63115480-63115499 Msa1115640:intron 35.0%
TCCAGTTCATTAGGATCTAA+TGG + chr7_3:63115484-63115503 None:intergenic 35.0%
! AATGAATAGGGTACTTTGGT+TGG + chr7_3:63115555-63115574 None:intergenic 35.0%
! GATGAATGAATAGGGTACTT+TGG + chr7_3:63115559-63115578 None:intergenic 35.0%
GTGTCTCTAAATTGATCATG+AGG - chr7_3:63115607-63115626 Msa1115640:intron 35.0%
ATCGACTAGAGAATTATCAG+TGG - chr7_3:63115669-63115688 Msa1115640:intron 35.0%
!!! TTTTATTTTGTGCCGGTGTT+AGG - chr7_3:63115779-63115798 Msa1115640:intron 35.0%
ATTTGCAGAAAACCTAACAC+CGG + chr7_3:63115794-63115813 None:intergenic 35.0%
TTCTACATCTGCTTATTTGC+AGG - chr7_3:63115897-63115916 Msa1115640:intron 35.0%
TCTACATCTGCTTATTTGCA+GGG - chr7_3:63115898-63115917 Msa1115640:intron 35.0%
!! AAAATGCAGGGTTAGTTTCT+AGG + chr7_3:63116069-63116088 None:intergenic 35.0%
CTACTATGCGTAACCAAATA+TGG - chr7_3:63116100-63116119 Msa1115640:intron 35.0%
! AGAAATGATCTTGTGCCATT+TGG - chr7_3:63116168-63116187 Msa1115640:intron 35.0%
AATGCTGCAAACATACATCA+AGG - chr7_3:63116483-63116502 Msa1115640:intron 35.0%
ACATACATCAAGGCTTCAAA+AGG - chr7_3:63116493-63116512 Msa1115640:intron 35.0%
AGAAGACTGGATAAAGACTA+AGG - chr7_3:63116517-63116536 Msa1115640:CDS 35.0%
TATGTCTAGTATGAACCACA+TGG - chr7_3:63116705-63116724 Msa1115640:exon 35.0%
!! GCTGTTCTTACTTTTCGAAG+TGG + chr7_3:63114460-63114479 None:intergenic 40.0%
TCATGGGTTTCCGATATTTC+TGG + chr7_3:63114556-63114575 None:intergenic 40.0%
ATTTAGGAGCTCCGAAGATT+CGG - chr7_3:63114579-63114598 Msa1115640:three_prime_UTR 40.0%
! TTTGGGATTCAAGAACCGAA+AGG + chr7_3:63114696-63114715 None:intergenic 40.0%
TTGAGGAAGAAAGGGTCTTT+GGG + chr7_3:63114713-63114732 None:intergenic 40.0%
!! CTGATTGTTGTGAGTTTTGG+AGG - chr7_3:63115230-63115249 Msa1115640:intron 40.0%
TGTTTGTGCCAGTAGTTTCT+TGG - chr7_3:63115319-63115338 Msa1115640:intron 40.0%
GTTTGTGCCAGTAGTTTCTT+GGG - chr7_3:63115320-63115339 Msa1115640:intron 40.0%
AATGAATGTGCACAAGCTGT+TGG + chr7_3:63115386-63115405 None:intergenic 40.0%
TCACAGATGTCCAGTTCATT+AGG + chr7_3:63115493-63115512 None:intergenic 40.0%
GCTTGGAGGATGAATGAATA+GGG + chr7_3:63115567-63115586 None:intergenic 40.0%
TGCTTGGAGGATGAATGAAT+AGG + chr7_3:63115568-63115587 None:intergenic 40.0%
!!! TGTTTTCAAGTGATGCTTGG+AGG + chr7_3:63115581-63115600 None:intergenic 40.0%
!!! AGCTGTTTTCAAGTGATGCT+TGG + chr7_3:63115584-63115603 None:intergenic 40.0%
CTACATCTGCTTATTTGCAG+GGG - chr7_3:63115899-63115918 Msa1115640:intron 40.0%
GCTGATGCAATCAAAATGCA+GGG + chr7_3:63116081-63116100 None:intergenic 40.0%
AGCTGATGCAATCAAAATGC+AGG + chr7_3:63116082-63116101 None:intergenic 40.0%
GCACAAGATCATTTCTGCTA+CGG + chr7_3:63116164-63116183 None:intergenic 40.0%
!! AATGATCTTGTGCCATTTGG+TGG - chr7_3:63116171-63116190 Msa1115640:intron 40.0%
AACAAGTCTTAGCCACCAAA+TGG + chr7_3:63116186-63116205 None:intergenic 40.0%
TTTACTTTCTCAGCACTCTC+TGG + chr7_3:63116363-63116382 None:intergenic 40.0%
CTTGCCAGTGTAGAATTGTA+CGG - chr7_3:63116542-63116561 Msa1115640:CDS 40.0%
TTTGCCGTACAATTCTACAC+TGG + chr7_3:63116549-63116568 None:intergenic 40.0%
!! GCTGTTCTTACTTTTCGAAG+TGG + chr7_3:63114460-63114479 None:intergenic 40.0%
TCATGGGTTTCCGATATTTC+TGG + chr7_3:63114556-63114575 None:intergenic 40.0%
ATTTAGGAGCTCCGAAGATT+CGG - chr7_3:63114579-63114598 Msa1115640:three_prime_UTR 40.0%
! TTTGGGATTCAAGAACCGAA+AGG + chr7_3:63114696-63114715 None:intergenic 40.0%
TTGAGGAAGAAAGGGTCTTT+GGG + chr7_3:63114713-63114732 None:intergenic 40.0%
!! CTGATTGTTGTGAGTTTTGG+AGG - chr7_3:63115230-63115249 Msa1115640:intron 40.0%
TGTTTGTGCCAGTAGTTTCT+TGG - chr7_3:63115319-63115338 Msa1115640:intron 40.0%
GTTTGTGCCAGTAGTTTCTT+GGG - chr7_3:63115320-63115339 Msa1115640:intron 40.0%
AATGAATGTGCACAAGCTGT+TGG + chr7_3:63115386-63115405 None:intergenic 40.0%
TCACAGATGTCCAGTTCATT+AGG + chr7_3:63115493-63115512 None:intergenic 40.0%
GCTTGGAGGATGAATGAATA+GGG + chr7_3:63115567-63115586 None:intergenic 40.0%
TGCTTGGAGGATGAATGAAT+AGG + chr7_3:63115568-63115587 None:intergenic 40.0%
!!! TGTTTTCAAGTGATGCTTGG+AGG + chr7_3:63115581-63115600 None:intergenic 40.0%
!!! AGCTGTTTTCAAGTGATGCT+TGG + chr7_3:63115584-63115603 None:intergenic 40.0%
CTACATCTGCTTATTTGCAG+GGG - chr7_3:63115899-63115918 Msa1115640:intron 40.0%
GCTGATGCAATCAAAATGCA+GGG + chr7_3:63116081-63116100 None:intergenic 40.0%
AGCTGATGCAATCAAAATGC+AGG + chr7_3:63116082-63116101 None:intergenic 40.0%
GCACAAGATCATTTCTGCTA+CGG + chr7_3:63116164-63116183 None:intergenic 40.0%
!! AATGATCTTGTGCCATTTGG+TGG - chr7_3:63116171-63116190 Msa1115640:intron 40.0%
AACAAGTCTTAGCCACCAAA+TGG + chr7_3:63116186-63116205 None:intergenic 40.0%
TTTACTTTCTCAGCACTCTC+TGG + chr7_3:63116363-63116382 None:intergenic 40.0%
CTTGCCAGTGTAGAATTGTA+CGG - chr7_3:63116542-63116561 Msa1115640:CDS 40.0%
TTTGCCGTACAATTCTACAC+TGG + chr7_3:63116549-63116568 None:intergenic 40.0%
! CGGAAACCCATGAAGCATTT+AGG - chr7_3:63114563-63114582 Msa1115640:three_prime_UTR 45.0%
GGAGCTCCTAAATGCTTCAT+GGG + chr7_3:63114572-63114591 None:intergenic 45.0%
CTCCTCTGAATCCGAATCTT+CGG + chr7_3:63114593-63114612 None:intergenic 45.0%
AGATTCGGATTCAGAGGAGT+TGG - chr7_3:63114594-63114613 Msa1115640:three_prime_UTR 45.0%
! GGAGTTGGAGTCTGATTTGA+AGG - chr7_3:63114609-63114628 Msa1115640:three_prime_UTR 45.0%
! AGAGTGGATTTGAGCTGATC+CGG + chr7_3:63114673-63114692 None:intergenic 45.0%
GCTCAAATCCACTCTCCTTT+CGG - chr7_3:63114678-63114697 Msa1115640:three_prime_UTR 45.0%
TTCAAGAACCGAAAGGAGAG+TGG + chr7_3:63114689-63114708 None:intergenic 45.0%
GTTGAGGAAGAAAGGGTCTT+TGG + chr7_3:63114714-63114733 None:intergenic 45.0%
TTACCCACCCAAGAAACTAC+TGG + chr7_3:63115330-63115349 None:intergenic 45.0%
GGCTTCAAAAGGAAGAAGAC+TGG - chr7_3:63116504-63116523 Msa1115640:intron 45.0%
AGTATGAACCACATGGTCCA+TGG - chr7_3:63116712-63116731 Msa1115640:five_prime_UTR 45.0%
! CGGAAACCCATGAAGCATTT+AGG - chr7_3:63114563-63114582 Msa1115640:three_prime_UTR 45.0%
GGAGCTCCTAAATGCTTCAT+GGG + chr7_3:63114572-63114591 None:intergenic 45.0%
CTCCTCTGAATCCGAATCTT+CGG + chr7_3:63114593-63114612 None:intergenic 45.0%
AGATTCGGATTCAGAGGAGT+TGG - chr7_3:63114594-63114613 Msa1115640:three_prime_UTR 45.0%
! GGAGTTGGAGTCTGATTTGA+AGG - chr7_3:63114609-63114628 Msa1115640:three_prime_UTR 45.0%
! AGAGTGGATTTGAGCTGATC+CGG + chr7_3:63114673-63114692 None:intergenic 45.0%
GCTCAAATCCACTCTCCTTT+CGG - chr7_3:63114678-63114697 Msa1115640:three_prime_UTR 45.0%
TTCAAGAACCGAAAGGAGAG+TGG + chr7_3:63114689-63114708 None:intergenic 45.0%
GTTGAGGAAGAAAGGGTCTT+TGG + chr7_3:63114714-63114733 None:intergenic 45.0%
TTACCCACCCAAGAAACTAC+TGG + chr7_3:63115330-63115349 None:intergenic 45.0%
GGCTTCAAAAGGAAGAAGAC+TGG - chr7_3:63116504-63116523 Msa1115640:intron 45.0%
AGTATGAACCACATGGTCCA+TGG - chr7_3:63116712-63116731 Msa1115640:five_prime_UTR 45.0%
CGGAGCTCCTAAATGCTTCA+TGG + chr7_3:63114573-63114592 None:intergenic 50.0%
CTCCGAAGATTCGGATTCAG+AGG - chr7_3:63114588-63114607 Msa1115640:three_prime_UTR 50.0%
TGTGCCAGTAGTTTCTTGGG+TGG - chr7_3:63115323-63115342 Msa1115640:intron 50.0%
GTGCCAGTAGTTTCTTGGGT+GGG - chr7_3:63115324-63115343 Msa1115640:intron 50.0%
ATCAGCTACCATGGACCATG+TGG + chr7_3:63116723-63116742 None:intergenic 50.0%
CGGAGCTCCTAAATGCTTCA+TGG + chr7_3:63114573-63114592 None:intergenic 50.0%
CTCCGAAGATTCGGATTCAG+AGG - chr7_3:63114588-63114607 Msa1115640:three_prime_UTR 50.0%
TGTGCCAGTAGTTTCTTGGG+TGG - chr7_3:63115323-63115342 Msa1115640:intron 50.0%
GTGCCAGTAGTTTCTTGGGT+GGG - chr7_3:63115324-63115343 Msa1115640:intron 50.0%
ATCAGCTACCATGGACCATG+TGG + chr7_3:63116723-63116742 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 63114435 63116818 63114435 ID=Msa1115640;Name=Msa1115640
chr7_3 mRNA 63114435 63116818 63114435 ID=Msa1115640-mRNA-1;Parent=Msa1115640;Name=Msa1115640-mRNA-1;_AED=0.01;_eAED=0.01;_QI=112|1|1|1|1|1|2|288|139
chr7_3 exon 63114435 63114946 63114435 ID=Msa1115640-mRNA-1:exon:14719;Parent=Msa1115640-mRNA-1
chr7_3 exon 63116511 63116818 63116511 ID=Msa1115640-mRNA-1:exon:14718;Parent=Msa1115640-mRNA-1
chr7_3 five_prime_UTR 63116707 63116818 63116707 ID=Msa1115640-mRNA-1:five_prime_utr;Parent=Msa1115640-mRNA-1
chr7_3 CDS 63116511 63116706 63116511 ID=Msa1115640-mRNA-1:cds;Parent=Msa1115640-mRNA-1
chr7_3 CDS 63114723 63114946 63114723 ID=Msa1115640-mRNA-1:cds;Parent=Msa1115640-mRNA-1
chr7_3 three_prime_UTR 63114435 63114722 63114435 ID=Msa1115640-mRNA-1:three_prime_utr;Parent=Msa1115640-mRNA-1
Gene Sequence

>Msa1115640

ATGGCTCCAGAAATATCGGAAACCCATGAAGCATTTAGGAGCTCCGAAGATTCGGATTCAGAGGAGTTGGAGTCTGATTTGAAGGAAATGTCGCAAAAGATTCTTGAATTTCGAACAACTCTTCCGGATCAGCTCAAATCCACTCTCCTTTCGGTTCTTGAATCCCAAAGACCCTTTCTTCCTCAACTCAATTCAGGTGCTTCAGAACAAAACATTTCTAGAGAGGGAAGTTCATCTACATCAGAAGATCCAGAGAGTGCTGAGAAAGTAAAATTACTAAAGGAAAAGATCTCAAGCAATTGCGCTGCTATGCCAATAGTTTTGAAAAGGATGAAAGATTGCATTGCGAAAATTGAAAAGTTAGATTCATATAATGCTGCAAACATACATCAAGGCTTCAAAAGGAAGAAGACTGGATAA
Protein sequence

>Msa1115640

MAPEISETHEAFRSSEDSDSEELESDLKEMSQKILEFRTTLPDQLKSTLLSVLESQRPFLPQLNSGASEQNISREGSSSTSEDPESAEKVKLLKEKISSNCAAMPIVLKRMKDCIAKIEKLDSYNAANIHQGFKRKKTG*