Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0458780 | XP_003599302.1 | 95.395 | 152 | 6 | 1 | 1 | 152 | 1 | 151 | 1.20e-97 | 288 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0458780 | G7J0N5 | 95.395 | 152 | 6 | 1 | 1 | 152 | 1 | 151 | 5.72e-98 | 288 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006880 | Msa0458780 | 0.829724 | 4.385719e-55 | -8.615850e-47 |
| Msa0007850 | Msa0458780 | 0.810482 | 1.127929e-50 | -8.615850e-47 |
| Msa0015680 | Msa0458780 | 0.812026 | 5.215505e-51 | -8.615850e-47 |
| Msa0026150 | Msa0458780 | 0.801317 | 9.532229e-49 | -8.615850e-47 |
| Msa0029260 | Msa0458780 | 0.819141 | 1.357513e-52 | -8.615850e-47 |
| Msa0031820 | Msa0458780 | 0.803390 | 3.567353e-49 | -8.615850e-47 |
| Msa0033880 | Msa0458780 | 0.842463 | 2.546824e-58 | -8.615850e-47 |
| Msa0047160 | Msa0458780 | 0.809614 | 1.734245e-50 | -8.615850e-47 |
| Msa0048180 | Msa0458780 | 0.807723 | 4.397469e-50 | -8.615850e-47 |
| Msa0055100 | Msa0458780 | 0.820685 | 6.020676e-53 | -8.615850e-47 |
| Msa0056030 | Msa0458780 | 0.814316 | 1.639062e-51 | -8.615850e-47 |
| Msa0057710 | Msa0458780 | 0.802759 | 4.817966e-49 | -8.615850e-47 |
| Msa0070960 | Msa0458780 | 0.803460 | 3.450247e-49 | -8.615850e-47 |
| Msa0081650 | Msa0458780 | 0.809126 | 2.207521e-50 | -8.615850e-47 |
| Msa0097950 | Msa0458780 | 0.835357 | 1.759049e-56 | -8.615850e-47 |
| Msa0098740 | Msa0458780 | 0.808326 | 3.272541e-50 | -8.615850e-47 |
| Msa0101180 | Msa0458780 | 0.800912 | 1.153535e-48 | -8.615850e-47 |
| Msa0104610 | Msa0458780 | 0.803306 | 3.713073e-49 | -8.615850e-47 |
| Msa0105520 | Msa0458780 | 0.800997 | 1.108406e-48 | -8.615850e-47 |
| Msa0108870 | Msa0458780 | 0.829913 | 3.944113e-55 | -8.615850e-47 |
| Msa0126770 | Msa0458780 | 0.815064 | 1.119339e-51 | -8.615850e-47 |
| Msa0127040 | Msa0458780 | 0.803175 | 3.952316e-49 | -8.615850e-47 |
| Msa0127850 | Msa0458780 | 0.835487 | 1.631132e-56 | -8.615850e-47 |
| Msa0128650 | Msa0458780 | 0.824591 | 7.431180e-54 | -8.615850e-47 |
| Msa0146770 | Msa0458780 | 0.801884 | 7.294498e-49 | -8.615850e-47 |
| Msa0165950 | Msa0458780 | 0.805817 | 1.111325e-49 | -8.615850e-47 |
| Msa0166750 | Msa0458780 | 0.810781 | 9.720245e-51 | -8.615850e-47 |
| Msa0169300 | Msa0458780 | 0.818187 | 2.234629e-52 | -8.615850e-47 |
| Msa0171850 | Msa0458780 | 0.811011 | 8.666418e-51 | -8.615850e-47 |
| Msa0174000 | Msa0458780 | 0.840074 | 1.082113e-57 | -8.615850e-47 |
| Msa0174690 | Msa0458780 | 0.818403 | 1.996422e-52 | -8.615850e-47 |
| Msa0177270 | Msa0458780 | 0.820210 | 7.735616e-53 | -8.615850e-47 |
| Msa0186770 | Msa0458780 | 0.810343 | 1.208393e-50 | -8.615850e-47 |
| Msa0198520 | Msa0458780 | 0.814804 | 1.278537e-51 | -8.615850e-47 |
| Msa0198530 | Msa0458780 | 0.825310 | 5.026731e-54 | -8.615850e-47 |
| Msa0221600 | Msa0458780 | 0.813543 | 2.426513e-51 | -8.615850e-47 |
| Msa0226330 | Msa0458780 | 0.802770 | 4.790726e-49 | -8.615850e-47 |
| Msa0231660 | Msa0458780 | 0.811326 | 7.405067e-51 | -8.615850e-47 |
| Msa0378970 | Msa0458780 | 0.805493 | 1.299699e-49 | -8.615850e-47 |
| Msa0384670 | Msa0458780 | 0.814778 | 1.295479e-51 | -8.615850e-47 |
| Msa0410300 | Msa0458780 | 0.812852 | 3.441853e-51 | -8.615850e-47 |
| Msa0413730 | Msa0458780 | 0.850112 | 2.096028e-60 | -8.615850e-47 |
| Msa0419520 | Msa0458780 | 0.841419 | 4.807728e-58 | -8.615850e-47 |
| Msa0434570 | Msa0458780 | 0.811032 | 8.573443e-51 | -8.615850e-47 |
| Msa0434770 | Msa0458780 | 0.814839 | 1.255740e-51 | -8.615850e-47 |
| Msa0439890 | Msa0458780 | 0.813266 | 2.792616e-51 | -8.615850e-47 |
| Msa0450140 | Msa0458780 | 0.800623 | 1.321552e-48 | -8.615850e-47 |
| Msa0458780 | Msa0461590 | 0.820879 | 5.433121e-53 | -8.615850e-47 |
| Msa0458780 | Msa0465550 | 0.826379 | 2.802367e-54 | -8.615850e-47 |
| Msa0458780 | Msa0474700 | 0.826177 | 3.130861e-54 | -8.615850e-47 |
| Msa0458780 | Msa0478580 | 0.826796 | 2.228231e-54 | -8.615850e-47 |
| Msa0458780 | Msa0479330 | 0.808623 | 2.827301e-50 | -8.615850e-47 |
| Msa0458780 | Msa0505380 | 0.849566 | 2.978291e-60 | -8.615850e-47 |
| Msa0458780 | Msa0511490 | 0.827541 | 1.477604e-54 | -8.615850e-47 |
| Msa0458780 | Msa0511770 | 0.802494 | 5.463033e-49 | -8.615850e-47 |
| Msa0458780 | Msa0546570 | 0.801503 | 8.730151e-49 | -8.615850e-47 |
| Msa0458780 | Msa0549590 | 0.812327 | 4.482690e-51 | -8.615850e-47 |
| Msa0458780 | Msa0550540 | 0.801917 | 7.181290e-49 | -8.615850e-47 |
| Msa0458780 | Msa0550570 | 0.809744 | 1.626759e-50 | -8.615850e-47 |
| Msa0458780 | Msa0565620 | -0.804567 | 2.030044e-49 | -8.615850e-47 |
| Msa0458780 | Msa0578090 | 0.801033 | 1.089578e-48 | -8.615850e-47 |
| Msa0458780 | Msa0586850 | 0.811696 | 6.153843e-51 | -8.615850e-47 |
| Msa0458780 | Msa0593700 | 0.801380 | 9.253335e-49 | -8.615850e-47 |
| Msa0458780 | Msa0609000 | 0.832695 | 8.163175e-56 | -8.615850e-47 |
| Msa0458780 | Msa0610390 | 0.805312 | 1.418516e-49 | -8.615850e-47 |
| Msa0458780 | Msa0614510 | 0.819733 | 9.945008e-53 | -8.615850e-47 |
| Msa0458780 | Msa0618930 | 0.816119 | 6.517815e-52 | -8.615850e-47 |
| Msa0458780 | Msa0618950 | 0.806579 | 7.679427e-50 | -8.615850e-47 |
| Msa0458780 | Msa0619920 | 0.820259 | 7.540851e-53 | -8.615850e-47 |
| Msa0458780 | Msa0620330 | 0.805732 | 1.157875e-49 | -8.615850e-47 |
| Msa0458780 | Msa0646130 | 0.804406 | 2.193733e-49 | -8.615850e-47 |
| Msa0458780 | Msa0652300 | 0.803503 | 3.379762e-49 | -8.615850e-47 |
| Msa0458780 | Msa0666230 | 0.815640 | 8.333789e-52 | -8.615850e-47 |
| Msa0458780 | Msa0679100 | 0.818192 | 2.228091e-52 | -8.615850e-47 |
| Msa0458780 | Msa0691690 | 0.812809 | 3.517558e-51 | -8.615850e-47 |
| Msa0458780 | Msa0694990 | 0.800185 | 1.623032e-48 | -8.615850e-47 |
| Msa0458780 | Msa0697720 | 0.804483 | 2.113545e-49 | -8.615850e-47 |
| Msa0458780 | Msa0706390 | 0.829668 | 4.526090e-55 | -8.615850e-47 |
| Msa0458780 | Msa0744090 | 0.819363 | 1.207960e-52 | -8.615850e-47 |
| Msa0458780 | Msa0744100 | 0.815673 | 8.193613e-52 | -8.615850e-47 |
| Msa0458780 | Msa0750920 | 0.812323 | 4.491297e-51 | -8.615850e-47 |
| Msa0458780 | Msa0756930 | 0.830594 | 2.690009e-55 | -8.615850e-47 |
| Msa0458780 | Msa0774750 | 0.803697 | 3.080104e-49 | -8.615850e-47 |
| Msa0458780 | Msa0780880 | 0.802814 | 4.692487e-49 | -8.615850e-47 |
| Msa0458780 | Msa0781270 | 0.820452 | 6.810169e-53 | -8.615850e-47 |
| Msa0458780 | Msa0795660 | 0.813787 | 2.144881e-51 | -8.615850e-47 |
| Msa0458780 | Msa0812290 | 0.806057 | 9.896600e-50 | -8.615850e-47 |
| Msa0458780 | Msa0819060 | 0.843328 | 1.499642e-58 | -8.615850e-47 |
| Msa0458780 | Msa0822170 | 0.805744 | 1.151483e-49 | -8.615850e-47 |
| Msa0458780 | Msa0822180 | 0.810919 | 9.071366e-51 | -8.615850e-47 |
| Msa0458780 | Msa0825270 | 0.819794 | 9.630682e-53 | -8.615850e-47 |
| Msa0458780 | Msa0825280 | 0.820864 | 5.475383e-53 | -8.615850e-47 |
| Msa0458780 | Msa0836500 | 0.814517 | 1.479660e-51 | -8.615850e-47 |
| Msa0458780 | Msa0839200 | 0.802118 | 6.529365e-49 | -8.615850e-47 |
| Msa0458780 | Msa0850710 | 0.802879 | 4.548963e-49 | -8.615850e-47 |
| Msa0458780 | Msa0851470 | 0.812227 | 4.714885e-51 | -8.615850e-47 |
| Msa0458780 | Msa0881580 | 0.815768 | 7.805220e-52 | -8.615850e-47 |
| Msa0458780 | Msa0888940 | 0.802899 | 4.507308e-49 | -8.615850e-47 |
| Msa0458780 | Msa0898890 | 0.821343 | 4.245675e-53 | -8.615850e-47 |
| Msa0458780 | Msa0899350 | 0.813332 | 2.700201e-51 | -8.615850e-47 |
| Msa0458780 | Msa0901450 | 0.811341 | 7.347417e-51 | -8.615850e-47 |
| Msa0458780 | Msa0943170 | 0.802013 | 6.860233e-49 | -8.615850e-47 |
| Msa0458780 | Msa0959910 | 0.810120 | 1.349597e-50 | -8.615850e-47 |
| Msa0458780 | Msa0960830 | 0.824056 | 9.923012e-54 | -8.615850e-47 |
| Msa0458780 | Msa0963020 | 0.812208 | 4.758164e-51 | -8.615850e-47 |
| Msa0458780 | Msa0965020 | 0.822486 | 2.309050e-53 | -8.615850e-47 |
| Msa0458780 | Msa0973320 | 0.811027 | 8.595230e-51 | -8.615850e-47 |
| Msa0458780 | Msa0976330 | 0.802870 | 4.569491e-49 | -8.615850e-47 |
| Msa0458780 | Msa0980940 | 0.824049 | 9.964019e-54 | -8.615850e-47 |
| Msa0458780 | Msa0981430 | 0.825463 | 4.623977e-54 | -8.615850e-47 |
| Msa0458780 | Msa1001350 | 0.824565 | 7.535899e-54 | -8.615850e-47 |
| Msa0458780 | Msa1019240 | 0.803283 | 3.753826e-49 | -8.615850e-47 |
| Msa0458780 | Msa1025940 | 0.838744 | 2.397705e-57 | -8.615850e-47 |
| Msa0458780 | Msa1027250 | 0.807700 | 4.446994e-50 | -8.615850e-47 |
| Msa0458780 | Msa1029970 | 0.826966 | 2.029062e-54 | -8.615850e-47 |
| Msa0458780 | Msa1030930 | 0.807866 | 4.099399e-50 | -8.615850e-47 |
| Msa0458780 | Msa1040800 | 0.808817 | 2.570723e-50 | -8.615850e-47 |
| Msa0458780 | Msa1041520 | 0.804271 | 2.340566e-49 | -8.615850e-47 |
| Msa0458780 | Msa1050420 | 0.817996 | 2.467857e-52 | -8.615850e-47 |
| Msa0458780 | Msa1070050 | 0.800163 | 1.639816e-48 | -8.615850e-47 |
| Msa0458780 | Msa1070390 | 0.813926 | 1.998836e-51 | -8.615850e-47 |
| Msa0458780 | Msa1093330 | 0.823485 | 1.350284e-53 | -8.615850e-47 |
| Msa0458780 | Msa1103660 | -0.802041 | 6.769971e-49 | -8.615850e-47 |
| Msa0458780 | Msa1115640 | 0.803106 | 4.084484e-49 | -8.615850e-47 |
| Msa0458780 | Msa1125550 | 0.804189 | 2.434198e-49 | -8.615850e-47 |
| Msa0458780 | Msa1133400 | 0.832816 | 7.618304e-56 | -8.615850e-47 |
| Msa0458780 | Msa1135800 | -0.804841 | 1.779997e-49 | -8.615850e-47 |
| Msa0458780 | Msa1152520 | 0.808120 | 3.619879e-50 | -8.615850e-47 |
| Msa0458780 | Msa1158230 | 0.806607 | 7.575702e-50 | -8.615850e-47 |
| Msa0458780 | Msa1179950 | 0.816462 | 5.463169e-52 | -8.615850e-47 |
| Msa0458780 | Msa1179980 | 0.824072 | 9.836738e-54 | -8.615850e-47 |
| Msa0458780 | Msa1192850 | 0.823696 | 1.205158e-53 | -8.615850e-47 |
| Msa0458780 | Msa1202150 | 0.806819 | 6.836446e-50 | -8.615850e-47 |
| Msa0458780 | Msa1202580 | 0.807745 | 4.351208e-50 | -8.615850e-47 |
| Msa0458780 | Msa1206310 | 0.812238 | 4.688430e-51 | -8.615850e-47 |
| Msa0458780 | Msa1206360 | 0.804946 | 1.691839e-49 | -8.615850e-47 |
| Msa0458780 | Msa1206650 | 0.803313 | 3.699587e-49 | -8.615850e-47 |
| Msa0458780 | Msa1207730 | 0.805357 | 1.388201e-49 | -8.615850e-47 |
| Msa0458780 | Msa1226610 | 0.855895 | 4.641240e-62 | -8.615850e-47 |
| Msa0458780 | Msa1254710 | 0.806345 | 8.605809e-50 | -8.615850e-47 |
| Msa0458780 | Msa1265630 | 0.809146 | 2.185709e-50 | -8.615850e-47 |
| Msa0458780 | Msa1270620 | 0.821142 | 4.723716e-53 | -8.615850e-47 |
| Msa0458780 | Msa1270690 | 0.823062 | 1.695681e-53 | -8.615850e-47 |
| Msa0458780 | Msa1294960 | 0.809621 | 1.728092e-50 | -8.615850e-47 |
| Msa0458780 | Msa1295110 | 0.805913 | 1.060784e-49 | -8.615850e-47 |
| Msa0458780 | Msa1306390 | 0.804366 | 2.236039e-49 | -8.615850e-47 |
| Msa0458780 | Msa1306990 | 0.823071 | 1.687593e-53 | -8.615850e-47 |
| Msa0458780 | Msa1323990 | 0.803730 | 3.032296e-49 | -8.615850e-47 |
| Msa0458780 | Msa1327490 | 0.821047 | 4.968737e-53 | -8.615850e-47 |
| Msa0458780 | Msa1331250 | 0.802883 | 4.540895e-49 | -8.615850e-47 |
| Msa0458780 | Msa1340830 | 0.804819 | 1.798607e-49 | -8.615850e-47 |
| Msa0458780 | Msa1355700 | 0.827276 | 1.711182e-54 | -8.615850e-47 |
| Msa0458780 | Msa1360980 | 0.803779 | 2.962250e-49 | -8.615850e-47 |
| Msa0458780 | Msa1388860 | 0.804319 | 2.286669e-49 | -8.615850e-47 |
| Msa0458780 | Msa1389850 | 0.813277 | 2.776452e-51 | -8.615850e-47 |
| Msa0458780 | Msa1397940 | 0.801632 | 8.216174e-49 | -8.615850e-47 |
| Msa0458780 | Msa1438050 | 0.808705 | 2.716315e-50 | -8.615850e-47 |
| Msa0244980 | Msa0458780 | 0.801844 | 7.431782e-49 | -8.615850e-47 |
| Msa0259270 | Msa0458780 | 0.813765 | 2.168422e-51 | -8.615850e-47 |
| Msa0278790 | Msa0458780 | 0.803915 | 2.775231e-49 | -8.615850e-47 |
| Msa0282890 | Msa0458780 | 0.809249 | 2.077139e-50 | -8.615850e-47 |
| Msa0291520 | Msa0458780 | 0.826323 | 2.889699e-54 | -8.615850e-47 |
| Msa0320610 | Msa0458780 | 0.820281 | 7.450293e-53 | -8.615850e-47 |
| Msa0321190 | Msa0458780 | 0.809903 | 1.503096e-50 | -8.615850e-47 |
| Msa0343190 | Msa0458780 | 0.819937 | 8.934925e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0458780 | MtrunA17_Chr3g0106271 | 95.395 | 152 | 6 | 1 | 1 | 152 | 1 | 151 | 1.10e-101 | 288 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0458780 | AT1G07020.1 | 52.000 | 150 | 60 | 5 | 9 | 150 | 1 | 146 | 1.41e-32 | 113 |
Find 55 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAAACACTTGAAGATT+TGG | 0.268474 | 3_3:-54848565 | Msa0458780:exon |
| TTGTTAACAAAATTGTGTTT+TGG | 0.342357 | 3_3:-54848464 | Msa0458780:three_prime_UTR |
| TCGTCGTCGACGGCGGATAT+TGG | 0.347800 | 3_3:+54848919 | None:intergenic |
| GGAGAACGGGATCTCGATAC+TGG | 0.356361 | 3_3:-54848824 | Msa0458780:CDS |
| AAGGGTGATGGAAGCGATGC+GGG | 0.371248 | 3_3:-54848674 | Msa0458780:CDS |
| AGAAGCTCCGAGATGGGTTT+CGG | 0.384931 | 3_3:-54848635 | Msa0458780:CDS |
| TCGAATTCTCATTTCAGCTT+TGG | 0.389985 | 3_3:-54848413 | Msa0458780:three_prime_UTR |
| TTAAGTTACAAGTGTCTGAT+TGG | 0.396536 | 3_3:+54848371 | None:intergenic |
| GCCACCATTGATTCAATTGA+AGG | 0.409218 | 3_3:-54849089 | Msa0458780:intron |
| TGAGGGAAGAGGAGAGAAGA+AGG | 0.410392 | 3_3:-54848726 | Msa0458780:CDS |
| GAGATGGGTTTCGGAAGTTC+CGG | 0.421286 | 3_3:-54848626 | Msa0458780:CDS |
| CGCACGGTGGAGCGGAGAAC+GGG | 0.430329 | 3_3:-54848837 | Msa0458780:CDS |
| CGACGACGAGGAGGAGGAAG+AGG | 0.448350 | 3_3:-54848794 | Msa0458780:CDS |
| TTAAAAGTCCTTAGATAAGA+TGG | 0.450145 | 3_3:-54848352 | Msa0458780:three_prime_UTR |
| TGATCAATCCAACGCTCCTC+CGG | 0.452257 | 3_3:+54848607 | None:intergenic |
| AATACCTTCAATTGAATCAA+TGG | 0.453135 | 3_3:+54849085 | None:intergenic |
| CGGAAGTTCCGGAGGAGCGT+TGG | 0.456212 | 3_3:-54848615 | Msa0458780:CDS |
| TGAGATTCATAGAGCATTGA+GGG | 0.462581 | 3_3:-54848743 | Msa0458780:CDS |
| GTCGCATTCTCCGCCGTCAA+CGG | 0.462597 | 3_3:+54848697 | None:intergenic |
| TGTAACCGTTCGGAATTTGA+TGG | 0.479310 | 3_3:+54848863 | None:intergenic |
| AAGAAGGATTGCACCGTTGA+CGG | 0.490658 | 3_3:-54848710 | Msa0458780:CDS |
| TCTGAACGACGACGAGGAGG+AGG | 0.509526 | 3_3:-54848800 | Msa0458780:CDS |
| TTGTTGGAGAAGCTCCGAGA+TGG | 0.515903 | 3_3:-54848642 | Msa0458780:CDS |
| GAGGAAGAGGAGGAGAGAAG+CGG | 0.520639 | 3_3:-54848781 | Msa0458780:CDS |
| TGAACTGCCGTCGTCGTCGA+CGG | 0.528246 | 3_3:+54848909 | None:intergenic |
| CGCGAATTCCGATGATGCTA+CGG | 0.534423 | 3_3:-54848953 | Msa0458780:CDS |
| CCGTGCGCAGTGTAACCGTT+CGG | 0.536665 | 3_3:+54848853 | None:intergenic |
| TGTTGGAGAAGCTCCGAGAT+GGG | 0.537143 | 3_3:-54848641 | Msa0458780:CDS |
| TCATAGAGCATTGAGGGAAG+AGG | 0.542007 | 3_3:-54848737 | Msa0458780:CDS |
| GTAGTATTCCGTAGCATCAT+CGG | 0.547237 | 3_3:+54848945 | None:intergenic |
| GCGCACGGTGGAGCGGAGAA+CGG | 0.567153 | 3_3:-54848838 | Msa0458780:CDS |
| GACGACGACGGCAGTTCAGA+CGG | 0.571091 | 3_3:-54848904 | Msa0458780:CDS |
| CGACGAGGAGGAGGAAGAGG+AGG | 0.573597 | 3_3:-54848791 | Msa0458780:CDS |
| CGTCGACGGCGGATATTGGT+TGG | 0.574955 | 3_3:+54848923 | None:intergenic |
| GGAGAATGCGACAAGGGTGA+TGG | 0.581303 | 3_3:-54848686 | Msa0458780:CDS |
| ATGAGATTCATAGAGCATTG+AGG | 0.585680 | 3_3:-54848744 | Msa0458780:CDS |
| GGGTGATGGAAGCGATGCGG+GGG | 0.586646 | 3_3:-54848672 | Msa0458780:CDS |
| AGGGTGATGGAAGCGATGCG+GGG | 0.587596 | 3_3:-54848673 | Msa0458780:CDS |
| TATTTGGTAGAAAACGGAAA+CGG | 0.589969 | 3_3:-54848988 | Msa0458780:intron |
| CAAGGGTGATGGAAGCGATG+CGG | 0.604617 | 3_3:-54848675 | Msa0458780:CDS |
| GGAACTTCCGAAACCCATCT+CGG | 0.606506 | 3_3:+54848628 | None:intergenic |
| ATGGGTTTCGGAAGTTCCGG+AGG | 0.614348 | 3_3:-54848623 | Msa0458780:CDS |
| GGATCTGAACGACGACGAGG+AGG | 0.620490 | 3_3:-54848803 | Msa0458780:CDS |
| GGATTGATCAGATTCGTAGA+CGG | 0.626249 | 3_3:-54848594 | Msa0458780:CDS |
| GATTTCCATCAAATTCCGAA+CGG | 0.629334 | 3_3:-54848868 | Msa0458780:CDS |
| TTACACTGCGCACGGTGGAG+CGG | 0.638902 | 3_3:-54848845 | Msa0458780:CDS |
| TGACGGCGGAGAATGCGACA+AGG | 0.639789 | 3_3:-54848693 | Msa0458780:CDS |
| CCGAACGGTTACACTGCGCA+CGG | 0.649523 | 3_3:-54848853 | Msa0458780:CDS |
| ATATCCGCCGTCGACGACGA+CGG | 0.654071 | 3_3:-54848916 | Msa0458780:CDS |
| GACGGCGGAGAATGCGACAA+GGG | 0.655697 | 3_3:-54848692 | Msa0458780:CDS |
| ACTGCCGTCGTCGTCGACGG+CGG | 0.659221 | 3_3:+54848912 | None:intergenic |
| ACCTTCAATTGAATCAATGG+TGG | 0.662332 | 3_3:+54849088 | None:intergenic |
| ACTGGATCTGAACGACGACG+AGG | 0.663751 | 3_3:-54848806 | Msa0458780:CDS |
| AAGGATTGCACCGTTGACGG+CGG | 0.674979 | 3_3:-54848707 | Msa0458780:CDS |
| AACGGTTACACTGCGCACGG+TGG | 0.709097 | 3_3:-54848850 | Msa0458780:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATTTTTGTTCTTTATT+TGG | - | chr3_3:54848484-54848503 | Msa0458780:three_prime_UTR | 10.0% |
| !!! | TTTTATTTTTGTTCTTTATT+TGG | - | chr3_3:54848484-54848503 | Msa0458780:three_prime_UTR | 10.0% |
| !!! | TTTGTTCTTCATTTTGATTA+TGG | - | chr3_3:54848967-54848986 | Msa0458780:CDS | 20.0% |
| !!! | TTGTTAACAAAATTGTGTTT+TGG | - | chr3_3:54849024-54849043 | Msa0458780:intron | 20.0% |
| !!! | TGTTAACAAAATTGTGTTTT+GGG | - | chr3_3:54849025-54849044 | Msa0458780:intron | 20.0% |
| !!! | TTTGTTCTTCATTTTGATTA+TGG | - | chr3_3:54848967-54848986 | Msa0458780:CDS | 20.0% |
| !!! | TTGTTAACAAAATTGTGTTT+TGG | - | chr3_3:54849024-54849043 | Msa0458780:intron | 20.0% |
| !!! | TGTTAACAAAATTGTGTTTT+GGG | - | chr3_3:54849025-54849044 | Msa0458780:intron | 20.0% |
| ! | AATACCTTCAATTGAATCAA+TGG | + | chr3_3:54848406-54848425 | None:intergenic | 25.0% |
| !! | GTTCTTTATTTGGTAGAAAA+CGG | - | chr3_3:54848494-54848513 | Msa0458780:three_prime_UTR | 25.0% |
| ! | TTAAAAGTCCTTAGATAAGA+TGG | - | chr3_3:54849136-54849155 | Msa0458780:five_prime_UTR | 25.0% |
| ! | AATACCTTCAATTGAATCAA+TGG | + | chr3_3:54848406-54848425 | None:intergenic | 25.0% |
| !! | GTTCTTTATTTGGTAGAAAA+CGG | - | chr3_3:54848494-54848513 | Msa0458780:three_prime_UTR | 25.0% |
| ! | TTAAAAGTCCTTAGATAAGA+TGG | - | chr3_3:54849136-54849155 | Msa0458780:five_prime_UTR | 25.0% |
| TATTTGGTAGAAAACGGAAA+CGG | - | chr3_3:54848500-54848519 | Msa0458780:three_prime_UTR | 30.0% | |
| TCTTCAAACACTTGAAGATT+TGG | - | chr3_3:54848923-54848942 | Msa0458780:CDS | 30.0% | |
| TTAAGTTACAAGTGTCTGAT+TGG | + | chr3_3:54849120-54849139 | None:intergenic | 30.0% | |
| TATTTGGTAGAAAACGGAAA+CGG | - | chr3_3:54848500-54848519 | Msa0458780:three_prime_UTR | 30.0% | |
| TCTTCAAACACTTGAAGATT+TGG | - | chr3_3:54848923-54848942 | Msa0458780:CDS | 30.0% | |
| TTAAGTTACAAGTGTCTGAT+TGG | + | chr3_3:54849120-54849139 | None:intergenic | 30.0% | |
| ACCTTCAATTGAATCAATGG+TGG | + | chr3_3:54848403-54848422 | None:intergenic | 35.0% | |
| GATTTCCATCAAATTCCGAA+CGG | - | chr3_3:54848620-54848639 | Msa0458780:CDS | 35.0% | |
| !! | ATGAGATTCATAGAGCATTG+AGG | - | chr3_3:54848744-54848763 | Msa0458780:CDS | 35.0% |
| !! | TGAGATTCATAGAGCATTGA+GGG | - | chr3_3:54848745-54848764 | Msa0458780:CDS | 35.0% |
| TCGAATTCTCATTTCAGCTT+TGG | - | chr3_3:54849075-54849094 | Msa0458780:intron | 35.0% | |
| ACCTTCAATTGAATCAATGG+TGG | + | chr3_3:54848403-54848422 | None:intergenic | 35.0% | |
| GATTTCCATCAAATTCCGAA+CGG | - | chr3_3:54848620-54848639 | Msa0458780:CDS | 35.0% | |
| !! | ATGAGATTCATAGAGCATTG+AGG | - | chr3_3:54848744-54848763 | Msa0458780:CDS | 35.0% |
| !! | TGAGATTCATAGAGCATTGA+GGG | - | chr3_3:54848745-54848764 | Msa0458780:CDS | 35.0% |
| TCGAATTCTCATTTCAGCTT+TGG | - | chr3_3:54849075-54849094 | Msa0458780:intron | 35.0% | |
| !! | GCCACCATTGATTCAATTGA+AGG | - | chr3_3:54848399-54848418 | Msa0458780:three_prime_UTR | 40.0% |
| GTAGTATTCCGTAGCATCAT+CGG | + | chr3_3:54848546-54848565 | None:intergenic | 40.0% | |
| TGTAACCGTTCGGAATTTGA+TGG | + | chr3_3:54848628-54848647 | None:intergenic | 40.0% | |
| GGATTGATCAGATTCGTAGA+CGG | - | chr3_3:54848894-54848913 | Msa0458780:CDS | 40.0% | |
| !! | GCCACCATTGATTCAATTGA+AGG | - | chr3_3:54848399-54848418 | Msa0458780:three_prime_UTR | 40.0% |
| GTAGTATTCCGTAGCATCAT+CGG | + | chr3_3:54848546-54848565 | None:intergenic | 40.0% | |
| TGTAACCGTTCGGAATTTGA+TGG | + | chr3_3:54848628-54848647 | None:intergenic | 40.0% | |
| GGATTGATCAGATTCGTAGA+CGG | - | chr3_3:54848894-54848913 | Msa0458780:CDS | 40.0% | |
| !! | TCATAGAGCATTGAGGGAAG+AGG | - | chr3_3:54848751-54848770 | Msa0458780:CDS | 45.0% |
| AAGAAGGATTGCACCGTTGA+CGG | - | chr3_3:54848778-54848797 | Msa0458780:CDS | 45.0% | |
| !! | TCATAGAGCATTGAGGGAAG+AGG | - | chr3_3:54848751-54848770 | Msa0458780:CDS | 45.0% |
| AAGAAGGATTGCACCGTTGA+CGG | - | chr3_3:54848778-54848797 | Msa0458780:CDS | 45.0% | |
| CGCGAATTCCGATGATGCTA+CGG | - | chr3_3:54848535-54848554 | Msa0458780:three_prime_UTR | 50.0% | |
| TGAGGGAAGAGGAGAGAAGA+AGG | - | chr3_3:54848762-54848781 | Msa0458780:CDS | 50.0% | |
| ATGCGGGGGATTTCGTTTGT+TGG | - | chr3_3:54848830-54848849 | Msa0458780:CDS | 50.0% | |
| TTGTTGGAGAAGCTCCGAGA+TGG | - | chr3_3:54848846-54848865 | Msa0458780:CDS | 50.0% | |
| TGTTGGAGAAGCTCCGAGAT+GGG | - | chr3_3:54848847-54848866 | Msa0458780:CDS | 50.0% | |
| ! | AGAAGCTCCGAGATGGGTTT+CGG | - | chr3_3:54848853-54848872 | Msa0458780:CDS | 50.0% |
| GGAACTTCCGAAACCCATCT+CGG | + | chr3_3:54848863-54848882 | None:intergenic | 50.0% | |
| ! | GAGATGGGTTTCGGAAGTTC+CGG | - | chr3_3:54848862-54848881 | Msa0458780:CDS | 50.0% |
| TGATCAATCCAACGCTCCTC+CGG | + | chr3_3:54848884-54848903 | None:intergenic | 50.0% | |
| CGCGAATTCCGATGATGCTA+CGG | - | chr3_3:54848535-54848554 | Msa0458780:three_prime_UTR | 50.0% | |
| TGAGGGAAGAGGAGAGAAGA+AGG | - | chr3_3:54848762-54848781 | Msa0458780:CDS | 50.0% | |
| ATGCGGGGGATTTCGTTTGT+TGG | - | chr3_3:54848830-54848849 | Msa0458780:CDS | 50.0% | |
| TTGTTGGAGAAGCTCCGAGA+TGG | - | chr3_3:54848846-54848865 | Msa0458780:CDS | 50.0% | |
| TGTTGGAGAAGCTCCGAGAT+GGG | - | chr3_3:54848847-54848866 | Msa0458780:CDS | 50.0% | |
| ! | AGAAGCTCCGAGATGGGTTT+CGG | - | chr3_3:54848853-54848872 | Msa0458780:CDS | 50.0% |
| GGAACTTCCGAAACCCATCT+CGG | + | chr3_3:54848863-54848882 | None:intergenic | 50.0% | |
| ! | GAGATGGGTTTCGGAAGTTC+CGG | - | chr3_3:54848862-54848881 | Msa0458780:CDS | 50.0% |
| TGATCAATCCAACGCTCCTC+CGG | + | chr3_3:54848884-54848903 | None:intergenic | 50.0% | |
| GGAGAACGGGATCTCGATAC+TGG | - | chr3_3:54848664-54848683 | Msa0458780:CDS | 55.0% | |
| ACTGGATCTGAACGACGACG+AGG | - | chr3_3:54848682-54848701 | Msa0458780:CDS | 55.0% | |
| GAGGAAGAGGAGGAGAGAAG+CGG | - | chr3_3:54848707-54848726 | Msa0458780:CDS | 55.0% | |
| AAGGATTGCACCGTTGACGG+CGG | - | chr3_3:54848781-54848800 | Msa0458780:CDS | 55.0% | |
| GGAGAATGCGACAAGGGTGA+TGG | - | chr3_3:54848802-54848821 | Msa0458780:CDS | 55.0% | |
| !! | CAAGGGTGATGGAAGCGATG+CGG | - | chr3_3:54848813-54848832 | Msa0458780:CDS | 55.0% |
| !! | AAGGGTGATGGAAGCGATGC+GGG | - | chr3_3:54848814-54848833 | Msa0458780:CDS | 55.0% |
| ATGGGTTTCGGAAGTTCCGG+AGG | - | chr3_3:54848865-54848884 | Msa0458780:CDS | 55.0% | |
| GGAGAACGGGATCTCGATAC+TGG | - | chr3_3:54848664-54848683 | Msa0458780:CDS | 55.0% | |
| ACTGGATCTGAACGACGACG+AGG | - | chr3_3:54848682-54848701 | Msa0458780:CDS | 55.0% | |
| GAGGAAGAGGAGGAGAGAAG+CGG | - | chr3_3:54848707-54848726 | Msa0458780:CDS | 55.0% | |
| AAGGATTGCACCGTTGACGG+CGG | - | chr3_3:54848781-54848800 | Msa0458780:CDS | 55.0% | |
| GGAGAATGCGACAAGGGTGA+TGG | - | chr3_3:54848802-54848821 | Msa0458780:CDS | 55.0% | |
| !! | CAAGGGTGATGGAAGCGATG+CGG | - | chr3_3:54848813-54848832 | Msa0458780:CDS | 55.0% |
| !! | AAGGGTGATGGAAGCGATGC+GGG | - | chr3_3:54848814-54848833 | Msa0458780:CDS | 55.0% |
| ATGGGTTTCGGAAGTTCCGG+AGG | - | chr3_3:54848865-54848884 | Msa0458780:CDS | 55.0% | |
| CGTCGACGGCGGATATTGGT+TGG | + | chr3_3:54848568-54848587 | None:intergenic | 60.0% | |
| TCGTCGTCGACGGCGGATAT+TGG | + | chr3_3:54848572-54848591 | None:intergenic | 60.0% | |
| ATATCCGCCGTCGACGACGA+CGG | - | chr3_3:54848572-54848591 | Msa0458780:exon | 60.0% | |
| TGAACTGCCGTCGTCGTCGA+CGG | + | chr3_3:54848582-54848601 | None:intergenic | 60.0% | |
| GACGACGACGGCAGTTCAGA+CGG | - | chr3_3:54848584-54848603 | Msa0458780:CDS | 60.0% | |
| CCGTGCGCAGTGTAACCGTT+CGG | + | chr3_3:54848638-54848657 | None:intergenic | 60.0% | |
| CCGAACGGTTACACTGCGCA+CGG | - | chr3_3:54848635-54848654 | Msa0458780:CDS | 60.0% | |
| AACGGTTACACTGCGCACGG+TGG | - | chr3_3:54848638-54848657 | Msa0458780:CDS | 60.0% | |
| TTACACTGCGCACGGTGGAG+CGG | - | chr3_3:54848643-54848662 | Msa0458780:CDS | 60.0% | |
| GGATCTGAACGACGACGAGG+AGG | - | chr3_3:54848685-54848704 | Msa0458780:CDS | 60.0% | |
| TCTGAACGACGACGAGGAGG+AGG | - | chr3_3:54848688-54848707 | Msa0458780:CDS | 60.0% | |
| GTCGCATTCTCCGCCGTCAA+CGG | + | chr3_3:54848794-54848813 | None:intergenic | 60.0% | |
| TGACGGCGGAGAATGCGACA+AGG | - | chr3_3:54848795-54848814 | Msa0458780:CDS | 60.0% | |
| GACGGCGGAGAATGCGACAA+GGG | - | chr3_3:54848796-54848815 | Msa0458780:CDS | 60.0% | |
| !! | AGGGTGATGGAAGCGATGCG+GGG | - | chr3_3:54848815-54848834 | Msa0458780:CDS | 60.0% |
| CGTCGACGGCGGATATTGGT+TGG | + | chr3_3:54848568-54848587 | None:intergenic | 60.0% | |
| TCGTCGTCGACGGCGGATAT+TGG | + | chr3_3:54848572-54848591 | None:intergenic | 60.0% | |
| ATATCCGCCGTCGACGACGA+CGG | - | chr3_3:54848572-54848591 | Msa0458780:exon | 60.0% | |
| TGAACTGCCGTCGTCGTCGA+CGG | + | chr3_3:54848582-54848601 | None:intergenic | 60.0% | |
| GACGACGACGGCAGTTCAGA+CGG | - | chr3_3:54848584-54848603 | Msa0458780:CDS | 60.0% | |
| CCGTGCGCAGTGTAACCGTT+CGG | + | chr3_3:54848638-54848657 | None:intergenic | 60.0% | |
| CCGAACGGTTACACTGCGCA+CGG | - | chr3_3:54848635-54848654 | Msa0458780:CDS | 60.0% | |
| AACGGTTACACTGCGCACGG+TGG | - | chr3_3:54848638-54848657 | Msa0458780:CDS | 60.0% | |
| TTACACTGCGCACGGTGGAG+CGG | - | chr3_3:54848643-54848662 | Msa0458780:CDS | 60.0% | |
| GGATCTGAACGACGACGAGG+AGG | - | chr3_3:54848685-54848704 | Msa0458780:CDS | 60.0% | |
| TCTGAACGACGACGAGGAGG+AGG | - | chr3_3:54848688-54848707 | Msa0458780:CDS | 60.0% | |
| GTCGCATTCTCCGCCGTCAA+CGG | + | chr3_3:54848794-54848813 | None:intergenic | 60.0% | |
| TGACGGCGGAGAATGCGACA+AGG | - | chr3_3:54848795-54848814 | Msa0458780:CDS | 60.0% | |
| GACGGCGGAGAATGCGACAA+GGG | - | chr3_3:54848796-54848815 | Msa0458780:CDS | 60.0% | |
| !! | AGGGTGATGGAAGCGATGCG+GGG | - | chr3_3:54848815-54848834 | Msa0458780:CDS | 60.0% |
| CGACGACGAGGAGGAGGAAG+AGG | - | chr3_3:54848694-54848713 | Msa0458780:CDS | 65.0% | |
| CGACGAGGAGGAGGAAGAGG+AGG | - | chr3_3:54848697-54848716 | Msa0458780:CDS | 65.0% | |
| !! | GGGTGATGGAAGCGATGCGG+GGG | - | chr3_3:54848816-54848835 | Msa0458780:CDS | 65.0% |
| ! | CGGAAGTTCCGGAGGAGCGT+TGG | - | chr3_3:54848873-54848892 | Msa0458780:CDS | 65.0% |
| CGACGACGAGGAGGAGGAAG+AGG | - | chr3_3:54848694-54848713 | Msa0458780:CDS | 65.0% | |
| CGACGAGGAGGAGGAAGAGG+AGG | - | chr3_3:54848697-54848716 | Msa0458780:CDS | 65.0% | |
| !! | GGGTGATGGAAGCGATGCGG+GGG | - | chr3_3:54848816-54848835 | Msa0458780:CDS | 65.0% |
| ! | CGGAAGTTCCGGAGGAGCGT+TGG | - | chr3_3:54848873-54848892 | Msa0458780:CDS | 65.0% |
| ACTGCCGTCGTCGTCGACGG+CGG | + | chr3_3:54848579-54848598 | None:intergenic | 70.0% | |
| GCGCACGGTGGAGCGGAGAA+CGG | - | chr3_3:54848650-54848669 | Msa0458780:CDS | 70.0% | |
| CGCACGGTGGAGCGGAGAAC+GGG | - | chr3_3:54848651-54848670 | Msa0458780:CDS | 70.0% | |
| ACTGCCGTCGTCGTCGACGG+CGG | + | chr3_3:54848579-54848598 | None:intergenic | 70.0% | |
| GCGCACGGTGGAGCGGAGAA+CGG | - | chr3_3:54848650-54848669 | Msa0458780:CDS | 70.0% | |
| CGCACGGTGGAGCGGAGAAC+GGG | - | chr3_3:54848651-54848670 | Msa0458780:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 54848350 | 54849160 | 54848350 | ID=Msa0458780;Name=Msa0458780 |
| chr3_3 | mRNA | 54848350 | 54849160 | 54848350 | ID=Msa0458780-mRNA-1;Parent=Msa0458780;Name=Msa0458780-mRNA-1;_AED=0.01;_eAED=0.01;_QI=40|1|1|1|1|1|2|223|152 |
| chr3_3 | exon | 54848350 | 54849000 | 54848350 | ID=Msa0458780-mRNA-1:exon:10858;Parent=Msa0458780-mRNA-1 |
| chr3_3 | exon | 54849090 | 54849160 | 54849090 | ID=Msa0458780-mRNA-1:exon:10857;Parent=Msa0458780-mRNA-1 |
| chr3_3 | five_prime_UTR | 54849121 | 54849160 | 54849121 | ID=Msa0458780-mRNA-1:five_prime_utr;Parent=Msa0458780-mRNA-1 |
| chr3_3 | CDS | 54849090 | 54849120 | 54849090 | ID=Msa0458780-mRNA-1:cds;Parent=Msa0458780-mRNA-1 |
| chr3_3 | CDS | 54848573 | 54849000 | 54848573 | ID=Msa0458780-mRNA-1:cds;Parent=Msa0458780-mRNA-1 |
| chr3_3 | three_prime_UTR | 54848350 | 54848572 | 54848350 | ID=Msa0458780-mRNA-1:three_prime_utr;Parent=Msa0458780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
