AlfalfaGEDB | Msa1125050

M. sativa cultivar zhongmu-4 / Msa1125050

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125050	XP_039684191.1	94.130	477	21	3	1	471	1	476	0.0	856

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125050	A0A396HCI7	94.130	477	21	3	1	471	1	476	0.0	856

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0052200	Msa1125050	0.810542	1.094332e-50	-8.615850e-47
Msa0097310	Msa1125050	0.806851	6.729695e-50	-8.615850e-47
Msa0143360	Msa1125050	0.817063	4.005353e-52	-8.615850e-47
Msa0207900	Msa1125050	0.808411	3.137792e-50	-8.615850e-47
Msa0307760	Msa1125050	0.838199	3.314283e-57	-8.615850e-47
Msa0331590	Msa1125050	0.812203	4.771793e-51	-8.615850e-47
Msa0907900	Msa1125050	0.837335	5.523897e-57	-8.615850e-47
Msa0946120	Msa1125050	0.802046	6.754496e-49	-8.615850e-47
Msa1041770	Msa1125050	0.932497	7.795309e-95	-8.615850e-47
Msa1042380	Msa1125050	0.812677	3.758525e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125050	MtrunA17_Chr7g0265901	94.130	477	21	3	1	471	1	476	0.0	856
Msa1125050	MtrunA17_Chr1g0179151	51.718	524	179	16	1	471	2	504	1.18e-136	403

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125050	AT5G03670.1	38.590	539	236	19	1	470	1	513	5.97e-74	242
Msa1125050	AT5G03670.2	38.053	339	133	14	1	273	1	328	1.38e-37	142

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Find 112 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTGGCAGTAAATTGT+AGG	0.334444	7_3:+76385724	None:intergenic
AACAAAGGCTTTGGGCTATT+TGG	0.129747	7_3:-76385004	Msa1125050:CDS
AAGAAGATGATGAATGTTTC+TGG	0.233486	7_3:+76385056	None:intergenic
AAGAATGCTGGATCAAGAAG+AGG	0.597514	7_3:-76382791	Msa1125050:CDS
AAGTTGGGTTAGGAAACCAA+GGG	0.677084	7_3:+76385584	None:intergenic
AATTAAGCAAATGAGACTTA+GGG	0.510109	7_3:+76382159	None:intergenic
AATTGATATGATGATAGAAG+AGG	0.596171	7_3:-76382497	Msa1125050:CDS
ACAGTAGGATTCTATAGGTT+AGG	0.501460	7_3:+76385779	None:intergenic
ACCTCAGTTATGTTTCGACT+CGG	0.610662	7_3:+76384677	None:intergenic
ACGAAACCAGAATCGGAAGC+AGG	0.517439	7_3:-76384957	Msa1125050:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAAATGGGAGAGAATTTGA+AGG	+	chr7_3:76382439-76382458	None:intergenic	30.0%
CTAACTAAGTTGTTGTGAAA+TGG	+	chr7_3:76382533-76382552	None:intergenic	30.0%
ATGTAGTTGTTGAGAAGAAA+TGG	+	chr7_3:76382612-76382631	None:intergenic	30.0%
AAGAAGATGATGAATGTTTC+TGG	+	chr7_3:76382869-76382888	None:intergenic	30.0%
TTCTCAGAAGAAAAACAACT+TGG	-	chr7_3:76383067-76383086	Msa1125050:intron	30.0%
GAAAAACAACTTGGAACATA+TGG	-	chr7_3:76383076-76383095	Msa1125050:intron	30.0%
TTTGTAGCAAATGAAACATG+TGG	+	chr7_3:76383537-76383556	None:intergenic	30.0%
AGTATCAATCAATTCAAGTC+AGG	-	chr7_3:76383870-76383889	Msa1125050:intron	30.0%
GTATCAATCAATTCAAGTCA+GGG	-	chr7_3:76383871-76383890	Msa1125050:intron	30.0%
TATCAATCAATTCAAGTCAG+GGG	-	chr7_3:76383872-76383891	Msa1125050:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	76382127	76385817	76382127	ID=Msa1125050;Name=Msa1125050
chr7_3	mRNA	76382127	76385817	76382127	ID=Msa1125050-mRNA-1;Parent=Msa1125050;Name=Msa1125050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=434\|1\|1\|1\|1\|1\|3\|245\|471
chr7_3	exon	76382127	76382889	76382127	ID=Msa1125050-mRNA-1:exon:20760;Parent=Msa1125050-mRNA-1
chr7_3	exon	76383909	76384101	76383909	ID=Msa1125050-mRNA-1:exon:20759;Parent=Msa1125050-mRNA-1
chr7_3	exon	76384679	76385817	76384679	ID=Msa1125050-mRNA-1:exon:20758;Parent=Msa1125050-mRNA-1
chr7_3	five_prime_UTR	76385384	76385817	76385384	ID=Msa1125050-mRNA-1:five_prime_utr;Parent=Msa1125050-mRNA-1
chr7_3	CDS	76384679	76385383	76384679	ID=Msa1125050-mRNA-1:cds;Parent=Msa1125050-mRNA-1
chr7_3	CDS	76383909	76384101	76383909	ID=Msa1125050-mRNA-1:cds;Parent=Msa1125050-mRNA-1
chr7_3	CDS	76382372	76382889	76382372	ID=Msa1125050-mRNA-1:cds;Parent=Msa1125050-mRNA-1
chr7_3	three_prime_UTR	76382127	76382371	76382127	ID=Msa1125050-mRNA-1:three_prime_utr;Parent=Msa1125050-mRNA-1

Gene Sequence

>Msa1125050

ATGATGGCTCCAAAATACTTACATGAGCTACTAAAAGAAGAGCAAGAACCATTTCTTCTCAACAACTACATTTCACATAAACGTAGCCAAATGAAAATCACTACTTTACAACTCAAGAAAAAACCATTCAACTCAAAATCAAACTTCAACATGAATCTTTGTAAAAACACTTCTTGTTTTTTCTCATTTCCCGAAACACCTGACTTCAAAAAATCACCACTGTTTGAACTAACTTCCCCTGTTAAAAGTCCCTGCAAAATCCCTTCTCGAACAGCAACACTTCTTCTTGAAGCTGCTCTCAGGATCCAGAAACATTCATCATCTTCTTCTTCTTCAAGTTCCAAAAACAAAACAAAAAACAAAGGCTTTGGGCTATTTGGTTCTTTGTTCAAGAAACTAACGCAACGAAACCAGAATCGGAAGCAGGAAATAGAGAATGAATATGTGTCTGTGAGGGAGTTATTGAAGTTGGATTCTTCTGTGAAGGTACGTAGAGAAAAAATGGATTCTCAGAAGAAAAACAACTTGGAACATATGGAAACTTCAAGTAGTGGTTACTCATTTGTTACTGATCATGATGCTTGTGCTTGTCGCCATCACCAACATGCTTTTCCTGAAAGTCCTTTTCGTTTTGCTCTTCAAACAAGTCCTTGCTCCGGCAGCCACACCCCGGAGTTAGCGTCGCCGAGTCGAAACATAACTGAGGACAAAGAAAGTAATGAAGCAGTTGAAAGTATCAATCAATTCAAGTCAGGGGAAGAAGAGGAGGAAGATAAGGAACAGTGTAGTCCAGTCTGTGTTTTAGACCCACCATTCGAGGATGATGATGAAGTACATATAAACGATGATGAAGATGGTGTTGATTTTGATTTAGAGAGCGGCTATGCCATTGTTCAGAGAGCAAGGCAGAAGATATTGTACAAGCTTTGCAGATTTGAGAAACTTGCAGAATTGGATCCATTAGAACTTGAGAAAAGAATGCTGGATCAAGAAGAGGATGAGGATGAAACATATATGGAAGAGGATGATGTTGAAGATGAGGAAAGAGAAGTATCATTTAAAGAAAATGACTTCAAAGAATTGGTTTTTGAAGCTGTGTATCTGTCAATGGTCCATGACAGACAGCAAATCCCACAAGAATTTAAGAAACTGATATCTGATCTAATCGTGGAAGAAGAGAGAGAACTCGATTCTTTAGAAGATAGCGACGTGGTGATAGCTAGGATATTGAAGAGGTTGGAATCATGGAAAGAAGTGGAGTCAAACACAATTGATATGATGATAGAAGAGGATTTCTCTATAGAGGATGGAGGGTGGAAGAAAAATGCTGAGCAGATAAGGAACATGGCTGGGGAGATTGAGCTTGCAATCTTTAGCATTCTGGTGGATGAATTTTCAGAGGAATTAGCATGTTAA

Protein sequence

>Msa1125050

MMAPKYLHELLKEEQEPFLLNNYISHKRSQMKITTLQLKKKPFNSKSNFNMNLCKNTSCFFSFPETPDFKKSPLFELTSPVKSPCKIPSRTATLLLEAALRIQKHSSSSSSSSSKNKTKNKGFGLFGSLFKKLTQRNQNRKQEIENEYVSVRELLKLDSSVKVRREKMDSQKKNNLEHMETSSSGYSFVTDHDACACRHHQHAFPESPFRFALQTSPCSGSHTPELASPSRNITEDKESNEAVESINQFKSGEEEEEDKEQCSPVCVLDPPFEDDDEVHINDDEDGVDFDLESGYAIVQRARQKILYKLCRFEKLAELDPLELEKRMLDQEEDEDETYMEEDDVEDEEREVSFKENDFKELVFEAVYLSMVHDRQQIPQEFKKLISDLIVEEERELDSLEDSDVVIARILKRLESWKEVESNTIDMMIEEDFSIEDGGWKKNAEQIRNMAGEIELAIFSILVDEFSEELAC*