AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1125050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125050 MtrunA17_Chr7g0265901 94.130 477 21 3 1 471 1 476 0.0 856
Msa1125050 MtrunA17_Chr1g0179151 51.718 524 179 16 1 471 2 504 1.18e-136 403
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125050 AT5G03670.1 38.590 539 236 19 1 470 1 513 5.97e-74 242
Msa1125050 AT5G03670.2 38.053 339 133 14 1 273 1 328 1.38e-37 142

Find 112 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAAGGCTTTGGGCTATT+TGG 0.129747 7_3:-76385004 Msa1125050:CDS
CTATTTCCTGCTTCCGATTC+TGG 0.143562 7_3:+76384951 None:intergenic
AGTAGCTCATGTAAGTATTT+TGG 0.148577 7_3:+76385352 None:intergenic
TAAACCCACATTATATTATT+TGG 0.168834 7_3:+76382222 None:intergenic
TCTAACATGGAATGTATTAT+TGG 0.229248 7_3:-76382284 Msa1125050:three_prime_UTR
AAGAAGATGATGAATGTTTC+TGG 0.233486 7_3:+76385056 None:intergenic
CAGAGGAATTAGCATGTTAA+TGG 0.248136 7_3:-76382369 Msa1125050:exon
GTCTACTACTACATAATAAA+TGG 0.254280 7_3:+76385654 None:intergenic
GACAGAATAAAATTAAAATT+TGG 0.262186 7_3:+76385710 None:intergenic
GCTTGCAATCTTTAGCATTC+TGG 0.276232 7_3:-76382404 Msa1125050:CDS
GCAAAACGAAAAGGACTTTC+AGG 0.307629 7_3:+76384749 None:intergenic
ATTTGAGAAACTTGCAGAAT+TGG 0.308653 7_3:-76382833 Msa1125050:CDS
AGAAAATGACTTCAAAGAAT+TGG 0.324338 7_3:-76382704 Msa1125050:CDS
AAAATTTGGCAGTAAATTGT+AGG 0.334444 7_3:+76385724 None:intergenic
GGATGAGGATGAAACATATA+TGG 0.353271 7_3:-76382770 Msa1125050:CDS
AGAGGAATTAGCATGTTAAT+GGG 0.362915 7_3:-76382368 Msa1125050:exon
CTAACTAAGTTGTTGTGAAA+TGG 0.368081 7_3:+76385392 None:intergenic
TAATTAAGCAAATGAGACTT+AGG 0.405389 7_3:+76382158 None:intergenic
CTAACATGGAATGTATTATT+GGG 0.405525 7_3:-76382283 Msa1125050:three_prime_UTR
GTGGGATTTGCTGTCTGTCA+TGG 0.410641 7_3:+76382653 None:intergenic
TTAGTTAGTTTATGTGATGA+TGG 0.419692 7_3:-76385377 Msa1125050:exon
AGTATCAATCAATTCAAGTC+AGG 0.420527 7_3:-76384052 Msa1125050:CDS
TCTTCTAATATAAAGAATAA+AGG 0.431130 7_3:+76382313 None:intergenic
AGTTGATTCAAATCTGAAGT+TGG 0.435953 7_3:+76385568 None:intergenic
TGTGAGGGAGTTATTGAAGT+TGG 0.436041 7_3:-76384912 Msa1125050:CDS
GTTGATTCAAATCTGAAGTT+GGG 0.437140 7_3:+76385569 None:intergenic
TAACTCCGGGGTGTGGCTGC+CGG 0.441342 7_3:+76384706 None:intergenic
TATTGAAGAGGTTGGAATCA+TGG 0.442336 7_3:-76382540 Msa1125050:CDS
ATTATTATTGTAAAATAATG+AGG 0.446933 7_3:+76385687 None:intergenic
TCAGTTTCTTAAATTCTTGT+GGG 0.447004 7_3:+76382635 None:intergenic
CTTGTACAGTAGGATTCTAT+AGG 0.450502 7_3:+76385774 None:intergenic
TTCTTCTTGAAGCTGCTCTC+AGG 0.452952 7_3:-76385081 Msa1125050:CDS
TTCAAATCTGAAGTTGGGTT+AGG 0.457886 7_3:+76385574 None:intergenic
ATCAGTTTCTTAAATTCTTG+TGG 0.459352 7_3:+76382634 None:intergenic
ATGTAGTTGTTGAGAAGAAA+TGG 0.459717 7_3:+76385313 None:intergenic
TGAAGCTGTGTATCTGTCAA+TGG 0.460059 7_3:-76382677 Msa1125050:CDS
AGCTAGGATATTGAAGAGGT+TGG 0.467698 7_3:-76382548 Msa1125050:CDS
AGCAGATAAGGAACATGGCT+GGG 0.477023 7_3:-76382435 Msa1125050:CDS
GGGGTGTGGCTGCCGGAGCA+AGG 0.478890 7_3:+76384713 None:intergenic
AGAAGAGGATTTCTCTATAG+AGG 0.482598 7_3:-76382482 Msa1125050:CDS
CGACTCGGCGACGCTAACTC+CGG 0.495649 7_3:+76384692 None:intergenic
ACAGTAGGATTCTATAGGTT+AGG 0.501460 7_3:+76385779 None:intergenic
TAACGCAACGAAACCAGAAT+CGG 0.502950 7_3:-76384964 Msa1125050:CDS
GGAAGAAGAGGAGGAAGATA+AGG 0.504402 7_3:-76384029 Msa1125050:CDS
CAGGAAAAGCATGTTGGTGA+TGG 0.505950 7_3:+76384768 None:intergenic
GTTTGAAGAGCAAAACGAAA+AGG 0.508198 7_3:+76384740 None:intergenic
AATTAAGCAAATGAGACTTA+GGG 0.510109 7_3:+76382159 None:intergenic
TGCAATCTTTAGCATTCTGG+TGG 0.515937 7_3:-76382401 Msa1125050:CDS
ACGAAACCAGAATCGGAAGC+AGG 0.517439 7_3:-76384957 Msa1125050:CDS
GAGCAGATAAGGAACATGGC+TGG 0.519541 7_3:-76382436 Msa1125050:CDS
ATGGAATGTATTATTGGGAT+AGG 0.523355 7_3:-76382278 Msa1125050:three_prime_UTR
GAAGTTGGATTCTTCTGTGA+AGG 0.523721 7_3:-76384897 Msa1125050:CDS
AGAACTTGAGAAAAGAATGC+TGG 0.525436 7_3:-76382803 Msa1125050:CDS
TACTTTCAGTCTTGTACAGT+AGG 0.531514 7_3:+76385764 None:intergenic
GGGGAGAGATGAGATGGGAT+GGG 0.539286 7_3:+76385606 None:intergenic
GGTGGGTCTAAAACACAGAC+TGG 0.539386 7_3:+76383995 None:intergenic
AGTGACCAAATAATATAATG+TGG 0.540524 7_3:-76382227 Msa1125050:three_prime_UTR
GCTGGATCAAGAAGAGGATG+AGG 0.543983 7_3:-76382785 Msa1125050:CDS
ATAGCGACGTGGTGATAGCT+AGG 0.544170 7_3:-76382564 Msa1125050:CDS
GCGACGCTAACTCCGGGGTG+TGG 0.547073 7_3:+76384699 None:intergenic
TTAAATGGGAGAGAATTTGA+AGG 0.549297 7_3:+76385486 None:intergenic
AGAAGTGTTGCTGTTCGAGA+AGG 0.550922 7_3:+76385100 None:intergenic
GAGGATTTCTCTATAGAGGA+TGG 0.553770 7_3:-76382478 Msa1125050:CDS
ATTCAAGTCAGGGGAAGAAG+AGG 0.558222 7_3:-76384041 Msa1125050:CDS
TCATCATCATCCTCGAATGG+TGG 0.561023 7_3:+76383977 None:intergenic
GAGGAATTAGCATGTTAATG+GGG 0.562269 7_3:-76382367 Msa1125050:exon
GTTGGAATCATGGAAAGAAG+TGG 0.563148 7_3:-76382530 Msa1125050:CDS
GGATGAAACATATATGGAAG+AGG 0.564977 7_3:-76382764 Msa1125050:CDS
CATATAAACGATGATGAAGA+TGG 0.565217 7_3:-76383950 Msa1125050:CDS
GACTTTCAGGAAAAGCATGT+TGG 0.568731 7_3:+76384762 None:intergenic
GAAGTGTTGCTGTTCGAGAA+GGG 0.568864 7_3:+76385101 None:intergenic
CATCATCATCCTCGAATGGT+GGG 0.573296 7_3:+76383978 None:intergenic
TTATTATTGTAAAATAATGA+GGG 0.577521 7_3:+76385688 None:intergenic
GTATCAATCAATTCAAGTCA+GGG 0.578564 7_3:-76384051 Msa1125050:CDS
GATTTCTCTATAGAGGATGG+AGG 0.589468 7_3:-76382475 Msa1125050:CDS
AATTGATATGATGATAGAAG+AGG 0.596171 7_3:-76382497 Msa1125050:CDS
AAGAATGCTGGATCAAGAAG+AGG 0.597514 7_3:-76382791 Msa1125050:CDS
CAAGTCAGGGGAAGAAGAGG+AGG 0.599409 7_3:-76384038 Msa1125050:CDS
GTGACCAAATAATATAATGT+GGG 0.603907 7_3:-76382226 Msa1125050:three_prime_UTR
TACAAGACTGAAAGTAGGAA+AGG 0.604462 7_3:-76385757 Msa1125050:five_prime_UTR
CTTCAAACAAGTCCTTGCTC+CGG 0.604522 7_3:-76384725 Msa1125050:CDS
CTCATGTTTGTAGCTAGATG+TGG 0.606774 7_3:-76382187 Msa1125050:three_prime_UTR
TTGCTCCGGCAGCCACACCC+CGG 0.609299 7_3:-76384711 Msa1125050:CDS
ACCTCAGTTATGTTTCGACT+CGG 0.610662 7_3:+76384677 None:intergenic
AGAGGATGATGTTGAAGATG+AGG 0.613307 7_3:-76382746 Msa1125050:CDS
CATATGGAAACTTCAAGTAG+TGG 0.617164 7_3:-76384830 Msa1125050:CDS
GCAGATAAGGAACATGGCTG+GGG 0.618512 7_3:-76382434 Msa1125050:CDS
GGATTCTATAGGTTAGGCGG+AGG 0.628968 7_3:+76385785 None:intergenic
ACTTCATCATCATCCTCGAA+TGG 0.634270 7_3:+76383974 None:intergenic
TAGAAGAGTATAGTCTAACA+TGG 0.635242 7_3:-76382297 Msa1125050:three_prime_UTR
ATAATGTGGGTTTATGTACA+TGG 0.637836 7_3:-76382213 Msa1125050:three_prime_UTR
TGCTGAGCAGATAAGGAACA+TGG 0.637996 7_3:-76382440 Msa1125050:CDS
TACTGTACAAGACTGAAAGT+AGG 0.639842 7_3:-76385762 Msa1125050:five_prime_UTR
TCTCTATAGAGGATGGAGGG+TGG 0.640291 7_3:-76382471 Msa1125050:CDS
TATCAATCAATTCAAGTCAG+GGG 0.642576 7_3:-76384050 Msa1125050:CDS
ACTGATATCTGATCTAATCG+TGG 0.648080 7_3:-76382617 Msa1125050:CDS
GAAGTTGGGTTAGGAAACCA+AGG 0.649026 7_3:+76385583 None:intergenic
ATTTCTCTATAGAGGATGGA+GGG 0.649835 7_3:-76382474 Msa1125050:CDS
GAATGAATATGTGTCTGTGA+GGG 0.651219 7_3:-76384927 Msa1125050:CDS
GTAGGATTCTATAGGTTAGG+CGG 0.651239 7_3:+76385782 None:intergenic
TGATAGCTAGGATATTGAAG+AGG 0.656936 7_3:-76382552 Msa1125050:CDS
AGGAATTAGCATGTTAATGG+GGG 0.657803 7_3:-76382366 Msa1125050:exon
CTGTGACATACTCTGAACAA+TGG 0.660476 7_3:+76383898 None:intergenic
TTGGGTTAGGAAACCAAGGG+GGG 0.667246 7_3:+76385587 None:intergenic
AAGTTGGGTTAGGAAACCAA+GGG 0.677084 7_3:+76385584 None:intergenic
GTTGGGTTAGGAAACCAAGG+GGG 0.680940 7_3:+76385586 None:intergenic
GAGATGGGATGGGAGTGAAG+TGG 0.693403 7_3:+76385616 None:intergenic
AGAATGAATATGTGTCTGTG+AGG 0.718238 7_3:-76384928 Msa1125050:CDS
GGGGAAGTTAGTTCAAACAG+TGG 0.724917 7_3:+76385145 None:intergenic
TTCTTTAGAAGATAGCGACG+TGG 0.740114 7_3:-76382575 Msa1125050:CDS
GCCGAGTCGAAACATAACTG+AGG 0.752254 7_3:-76384678 Msa1125050:intron
AGTTGGGTTAGGAAACCAAG+GGG 0.772580 7_3:+76385585 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTTTTTTTTTATTT+TGG - chr7_3:76383294-76383313 Msa1125050:intron 0.0%
!! TTATTATTGTAAAATAATGA+GGG + chr7_3:76382237-76382256 None:intergenic 10.0%
!! ATTATTATTGTAAAATAATG+AGG + chr7_3:76382238-76382257 None:intergenic 10.0%
!!! TTTTCAATAATAAAAAGTTT+AGG + chr7_3:76384299-76384318 None:intergenic 10.0%
!!! AATTTTATAAAACTTGATAA+GGG + chr7_3:76384600-76384619 None:intergenic 10.0%
!!! AAATTTTATAAAACTTGATA+AGG + chr7_3:76384601-76384620 None:intergenic 10.0%
!! GACAGAATAAAATTAAAATT+TGG + chr7_3:76382215-76382234 None:intergenic 15.0%
!!! TTTGTTTTTTGTTTTGTTTT+TGG + chr7_3:76382904-76382923 None:intergenic 15.0%
!! AAAAACAAAACAAAAAACAA+AGG - chr7_3:76382903-76382922 Msa1125050:intron 15.0%
!! TCTTCTAATATAAAGAATAA+AGG + chr7_3:76385612-76385631 None:intergenic 15.0%
!!! TGAAGATGTTTTGTTTAAAT+GGG + chr7_3:76382453-76382472 None:intergenic 20.0%
!!! TTGAAGATGTTTTGTTTAAA+TGG + chr7_3:76382454-76382473 None:intergenic 20.0%
!!! TTTTCGTTTTGAATGTTTAA+TGG - chr7_3:76383340-76383359 Msa1125050:intron 20.0%
!!! TGATTGTTTGTTTGTTTTTT+CGG - chr7_3:76383434-76383453 Msa1125050:intron 20.0%
!!! TTATTGACTCATTTTTTGTA+GGG + chr7_3:76383611-76383630 None:intergenic 20.0%
!!! TTTATTGACTCATTTTTTGT+AGG + chr7_3:76383612-76383631 None:intergenic 20.0%
!!! TTATTTAATGAGAGTTTTTG+TGG - chr7_3:76383808-76383827 Msa1125050:intron 20.0%
!!! GTTTTTGTGGAATTTAATAT+AGG - chr7_3:76383821-76383840 Msa1125050:intron 20.0%
!! ATACAATCAACAAATTAGAA+TGG + chr7_3:76384070-76384089 None:intergenic 20.0%
!!! ATTCTAATTTGTTGATTGTA+TGG - chr7_3:76384069-76384088 Msa1125050:CDS 20.0%
!!! ACTTTTTTGTTTGTTTTGAT+TGG - chr7_3:76384626-76384645 Msa1125050:intron 20.0%
!! TGTAAAATTACTTGTCTTAA+GGG + chr7_3:76384793-76384812 None:intergenic 20.0%
!! ATGTAAAATTACTTGTCTTA+AGG + chr7_3:76384794-76384813 None:intergenic 20.0%
!!! CAAGTAATTTTACATATGTA+CGG - chr7_3:76384799-76384818 Msa1125050:CDS 20.0%
!!! ATTTTGAAACTAAGTATGAA+TGG - chr7_3:76385582-76385601 Msa1125050:five_prime_UTR 20.0%
!! TAAACCCACATTATATTATT+TGG + chr7_3:76385703-76385722 None:intergenic 20.0%
! AAAATTTGGCAGTAAATTGT+AGG + chr7_3:76382201-76382220 None:intergenic 25.0%
! GTCTACTACTACATAATAAA+TGG + chr7_3:76382271-76382290 None:intergenic 25.0%
!! TTTTGTATTGTTAGTTAGAG+TGG + chr7_3:76382390-76382409 None:intergenic 25.0%
!! TTAGTTAGTTTATGTGATGA+TGG - chr7_3:76382545-76382564 Msa1125050:CDS 25.0%
!! GTAAAGTAGTGATTTTCATT+TGG + chr7_3:76382650-76382669 None:intergenic 25.0%
!!! AAGTTTGATTTTGAGTTGAA+TGG + chr7_3:76382687-76382706 None:intergenic 25.0%
! AAAACAAAAAACAAAGGCTT+TGG - chr7_3:76382909-76382928 Msa1125050:intron 25.0%
! AAACAAAAAACAAAGGCTTT+GGG - chr7_3:76382910-76382929 Msa1125050:intron 25.0%
! AAGTACAATCATCAAAATTC+TGG + chr7_3:76383375-76383394 None:intergenic 25.0%
! TTGTACAAAAACACATTAAG+AGG - chr7_3:76384122-76384141 Msa1125050:intron 25.0%
! AAAAGTTTAGGTGAATTTGA+TGG + chr7_3:76384287-76384306 None:intergenic 25.0%
! TGAAAAACAAACACATTTAG+TGG - chr7_3:76384313-76384332 Msa1125050:intron 25.0%
! GAAAAACAAACACATTTAGT+GGG - chr7_3:76384314-76384333 Msa1125050:intron 25.0%
! AAAAACAAACACATTTAGTG+GGG - chr7_3:76384315-76384334 Msa1125050:intron 25.0%
!! CTCCTAAAATGAAATTTTGT+GGG + chr7_3:76384519-76384538 None:intergenic 25.0%
!! TCTCCTAAAATGAAATTTTG+TGG + chr7_3:76384520-76384539 None:intergenic 25.0%
! AGATCTTTCAATCAATATGT+TGG - chr7_3:76384553-76384572 Msa1125050:intron 25.0%
! TGACCTAATTAATACTCATT+GGG - chr7_3:76384697-76384716 Msa1125050:CDS 25.0%
! TATCCCAATGAGTATTAATT+AGG + chr7_3:76384703-76384722 None:intergenic 25.0%
!! ATTTTGTGGTACCTTAAATT+TGG - chr7_3:76384724-76384743 Msa1125050:CDS 25.0%
! ATTCTCAACAACCAAATTTA+AGG + chr7_3:76384738-76384757 None:intergenic 25.0%
!! ATCTAACCAAGATATTTTAC+AGG - chr7_3:76385033-76385052 Msa1125050:CDS 25.0%
!!! ATTCTTTTCTCAAGTTCTAA+TGG + chr7_3:76385117-76385136 None:intergenic 25.0%
! AGAAAATGACTTCAAAGAAT+TGG - chr7_3:76385218-76385237 Msa1125050:CDS 25.0%
! TCAGTTTCTTAAATTCTTGT+GGG + chr7_3:76385290-76385309 None:intergenic 25.0%
! ATCAGTTTCTTAAATTCTTG+TGG + chr7_3:76385291-76385310 None:intergenic 25.0%
! AATTGATATGATGATAGAAG+AGG - chr7_3:76385425-76385444 Msa1125050:five_prime_UTR 25.0%
! TCTAACATGGAATGTATTAT+TGG - chr7_3:76385638-76385657 Msa1125050:five_prime_UTR 25.0%
! CTAACATGGAATGTATTATT+GGG - chr7_3:76385639-76385658 Msa1125050:five_prime_UTR 25.0%
! AGTGACCAAATAATATAATG+TGG - chr7_3:76385695-76385714 Msa1125050:five_prime_UTR 25.0%
! GTGACCAAATAATATAATGT+GGG - chr7_3:76385696-76385715 Msa1125050:five_prime_UTR 25.0%
! AATTAAGCAAATGAGACTTA+GGG + chr7_3:76385766-76385785 None:intergenic 25.0%
! TAATTAAGCAAATGAGACTT+AGG + chr7_3:76385767-76385786 None:intergenic 25.0%
! GTTGATTCAAATCTGAAGTT+GGG + chr7_3:76382356-76382375 None:intergenic 30.0%
! AGTTGATTCAAATCTGAAGT+TGG + chr7_3:76382357-76382376 None:intergenic 30.0%
TTAAATGGGAGAGAATTTGA+AGG + chr7_3:76382439-76382458 None:intergenic 30.0%
CTAACTAAGTTGTTGTGAAA+TGG + chr7_3:76382533-76382552 None:intergenic 30.0%
! AGTAGCTCATGTAAGTATTT+TGG + chr7_3:76382573-76382592 None:intergenic 30.0%
ATGTAGTTGTTGAGAAGAAA+TGG + chr7_3:76382612-76382631 None:intergenic 30.0%
!!! TTTTTTGAAGTCAGGTGTTT+CGG + chr7_3:76382754-76382773 None:intergenic 30.0%
AAGAAGATGATGAATGTTTC+TGG + chr7_3:76382869-76382888 None:intergenic 30.0%
TTCTCAGAAGAAAAACAACT+TGG - chr7_3:76383067-76383086 Msa1125050:intron 30.0%
GAAAAACAACTTGGAACATA+TGG - chr7_3:76383076-76383095 Msa1125050:intron 30.0%
TTTGTAGCAAATGAAACATG+TGG + chr7_3:76383537-76383556 None:intergenic 30.0%
AGTATCAATCAATTCAAGTC+AGG - chr7_3:76383870-76383889 Msa1125050:intron 30.0%
GTATCAATCAATTCAAGTCA+GGG - chr7_3:76383871-76383890 Msa1125050:intron 30.0%
TATCAATCAATTCAAGTCAG+GGG - chr7_3:76383872-76383891 Msa1125050:intron 30.0%
CATATAAACGATGATGAAGA+TGG - chr7_3:76383972-76383991 Msa1125050:CDS 30.0%
!!! GTTGATTTTGATTTAGAGAG+CGG - chr7_3:76383996-76384015 Msa1125050:CDS 30.0%
ATTCAACTCAAATCCTACAA+GGG - chr7_3:76384168-76384187 Msa1125050:intron 30.0%
TTATATATCACCTATCAACG+AGG + chr7_3:76384205-76384224 None:intergenic 30.0%
TGTAGTTGTAAACAAAGAGT+AGG + chr7_3:76384464-76384483 None:intergenic 30.0%
! GACCCACAAAATTTCATTTT+AGG - chr7_3:76384514-76384533 Msa1125050:intron 30.0%
!!! TGTTTTGATTGGCAAATTGT+AGG - chr7_3:76384637-76384656 Msa1125050:intron 30.0%
! TAGGATGTTGGTAAATTAGT+TGG - chr7_3:76384656-76384675 Msa1125050:intron 30.0%
GTGACCTAATTAATACTCAT+TGG - chr7_3:76384696-76384715 Msa1125050:CDS 30.0%
! ACTCATTGGGATAAATTTTG+TGG - chr7_3:76384710-76384729 Msa1125050:CDS 30.0%
TATGGTACATGTAGCATTAT+AGG + chr7_3:76384770-76384789 None:intergenic 30.0%
AATTACTTGTCTTAAGGGTA+TGG + chr7_3:76384788-76384807 None:intergenic 30.0%
GAATAGAACAGTAAACTGAT+TGG + chr7_3:76384830-76384849 None:intergenic 30.0%
ATTTGAGAAACTTGCAGAAT+TGG - chr7_3:76385089-76385108 Msa1125050:CDS 30.0%
AGAGGAATTAGCATGTTAAT+GGG - chr7_3:76385554-76385573 Msa1125050:five_prime_UTR 30.0%
! TAGAAGAGTATAGTCTAACA+TGG - chr7_3:76385625-76385644 Msa1125050:five_prime_UTR 30.0%
ATGGAATGTATTATTGGGAT+AGG - chr7_3:76385644-76385663 Msa1125050:five_prime_UTR 30.0%
ATAATGTGGGTTTATGTACA+TGG - chr7_3:76385709-76385728 Msa1125050:five_prime_UTR 30.0%
ACAGTAGGATTCTATAGGTT+AGG + chr7_3:76382146-76382165 None:intergenic 35.0%
CTTGTACAGTAGGATTCTAT+AGG + chr7_3:76382151-76382170 None:intergenic 35.0%
! TACTTTCAGTCTTGTACAGT+AGG + chr7_3:76382161-76382180 None:intergenic 35.0%
TACTGTACAAGACTGAAAGT+AGG - chr7_3:76382160-76382179 Msa1125050:three_prime_UTR 35.0%
TACAAGACTGAAAGTAGGAA+AGG - chr7_3:76382165-76382184 Msa1125050:three_prime_UTR 35.0%
! TTCAAATCTGAAGTTGGGTT+AGG + chr7_3:76382351-76382370 None:intergenic 35.0%
!!! TTTTTGAAGTCAGGTGTTTC+GGG + chr7_3:76382753-76382772 None:intergenic 35.0%
!!! AGTGGTGATTTTTTGAAGTC+AGG + chr7_3:76382762-76382781 None:intergenic 35.0%
! TTTTGCAGGGACTTTTAACA+GGG + chr7_3:76382800-76382819 None:intergenic 35.0%
! ATTTTGCAGGGACTTTTAAC+AGG + chr7_3:76382801-76382820 None:intergenic 35.0%
AGAATGAATATGTGTCTGTG+AGG - chr7_3:76382994-76383013 Msa1125050:intron 35.0%
GAATGAATATGTGTCTGTGA+GGG - chr7_3:76382995-76383014 Msa1125050:intron 35.0%
! GAAGGTACGTAGAGAAAAAA+TGG - chr7_3:76383043-76383062 Msa1125050:intron 35.0%
CATATGGAAACTTCAAGTAG+TGG - chr7_3:76383092-76383111 Msa1125050:intron 35.0%
GTTTGAAGAGCAAAACGAAA+AGG + chr7_3:76383185-76383204 None:intergenic 35.0%
GATTCAACTCAAATCCTACA+AGG - chr7_3:76384167-76384186 Msa1125050:intron 35.0%
ATTGGCAAATTGTAGGATGT+TGG - chr7_3:76384644-76384663 Msa1125050:intron 35.0%
CATACTTTCACTCGACAATA+TGG - chr7_3:76384854-76384873 Msa1125050:CDS 35.0%
CTTGCTCCTGTAAAATATCT+TGG + chr7_3:76385042-76385061 None:intergenic 35.0%
! CAAGATATTTTACAGGAGCA+AGG - chr7_3:76385040-76385059 Msa1125050:CDS 35.0%
AGAACTTGAGAAAAGAATGC+TGG - chr7_3:76385119-76385138 Msa1125050:CDS 35.0%
GGATGAGGATGAAACATATA+TGG - chr7_3:76385152-76385171 Msa1125050:CDS 35.0%
GGATGAAACATATATGGAAG+AGG - chr7_3:76385158-76385177 Msa1125050:CDS 35.0%
ACTGATATCTGATCTAATCG+TGG - chr7_3:76385305-76385324 Msa1125050:CDS 35.0%
TGATAGCTAGGATATTGAAG+AGG - chr7_3:76385370-76385389 Msa1125050:exon 35.0%
TATTGAAGAGGTTGGAATCA+TGG - chr7_3:76385382-76385401 Msa1125050:exon 35.0%
AGAAGAGGATTTCTCTATAG+AGG - chr7_3:76385440-76385459 Msa1125050:five_prime_UTR 35.0%
ATTTCTCTATAGAGGATGGA+GGG - chr7_3:76385448-76385467 Msa1125050:five_prime_UTR 35.0%
AGAAAAATGCTGAGCAGATA+AGG - chr7_3:76385475-76385494 Msa1125050:five_prime_UTR 35.0%
CAGAGGAATTAGCATGTTAA+TGG - chr7_3:76385553-76385572 Msa1125050:five_prime_UTR 35.0%
GAGGAATTAGCATGTTAATG+GGG - chr7_3:76385555-76385574 Msa1125050:five_prime_UTR 35.0%
AGGAATTAGCATGTTAATGG+GGG - chr7_3:76385556-76385575 Msa1125050:five_prime_UTR 35.0%
GTAGGATTCTATAGGTTAGG+CGG + chr7_3:76382143-76382162 None:intergenic 40.0%
! AAGTTGGGTTAGGAAACCAA+GGG + chr7_3:76382341-76382360 None:intergenic 40.0%
! TTTGCAGGGACTTTTAACAG+GGG + chr7_3:76382799-76382818 None:intergenic 40.0%
!! AACAAAGGCTTTGGGCTATT+TGG - chr7_3:76382918-76382937 Msa1125050:intron 40.0%
TAACGCAACGAAACCAGAAT+CGG - chr7_3:76382958-76382977 Msa1125050:intron 40.0%
TGTGAGGGAGTTATTGAAGT+TGG - chr7_3:76383010-76383029 Msa1125050:intron 40.0%
GAAGTTGGATTCTTCTGTGA+AGG - chr7_3:76383025-76383044 Msa1125050:intron 40.0%
! GACTTTCAGGAAAAGCATGT+TGG + chr7_3:76383163-76383182 None:intergenic 40.0%
GCAAAACGAAAAGGACTTTC+AGG + chr7_3:76383176-76383195 None:intergenic 40.0%
ACCTCAGTTATGTTTCGACT+CGG + chr7_3:76383248-76383267 None:intergenic 40.0%
CAGAGAGAGAAAGATTTGCA+TGG + chr7_3:76383414-76383433 None:intergenic 40.0%
ACTTCATCATCATCCTCGAA+TGG + chr7_3:76383951-76383970 None:intergenic 40.0%
CTGTGACATACTCTGAACAA+TGG + chr7_3:76384027-76384046 None:intergenic 40.0%
AACACATTAAGAGGCAACCT+TGG - chr7_3:76384131-76384150 Msa1125050:intron 40.0%
GAGTTGAATCCTTACCAACA+AGG + chr7_3:76384157-76384176 None:intergenic 40.0%
ACAGCTGCTCAACATGTAAA+AGG - chr7_3:76384405-76384424 Msa1125050:intron 40.0%
! AAGGCATTTCCCATTCAATG+TGG - chr7_3:76384424-76384443 Msa1125050:intron 40.0%
TTTCCCATTCAATGTGGACT+TGG - chr7_3:76384430-76384449 Msa1125050:intron 40.0%
TAAACAAAGAGTAGGCCACT+AGG + chr7_3:76384456-76384475 None:intergenic 40.0%
AAGAATGCTGGATCAAGAAG+AGG - chr7_3:76385131-76385150 Msa1125050:CDS 40.0%
AGAGGATGATGTTGAAGATG+AGG - chr7_3:76385176-76385195 Msa1125050:CDS 40.0%
TGAAGCTGTGTATCTGTCAA+TGG - chr7_3:76385245-76385264 Msa1125050:CDS 40.0%
! TTCTTTAGAAGATAGCGACG+TGG - chr7_3:76385347-76385366 Msa1125050:CDS 40.0%
AGCTAGGATATTGAAGAGGT+TGG - chr7_3:76385374-76385393 Msa1125050:exon 40.0%
GTTGGAATCATGGAAAGAAG+TGG - chr7_3:76385392-76385411 Msa1125050:five_prime_UTR 40.0%
GAGGATTTCTCTATAGAGGA+TGG - chr7_3:76385444-76385463 Msa1125050:five_prime_UTR 40.0%
GATTTCTCTATAGAGGATGG+AGG - chr7_3:76385447-76385466 Msa1125050:five_prime_UTR 40.0%
GCTTGCAATCTTTAGCATTC+TGG - chr7_3:76385518-76385537 Msa1125050:five_prime_UTR 40.0%
TGCAATCTTTAGCATTCTGG+TGG - chr7_3:76385521-76385540 Msa1125050:five_prime_UTR 40.0%
!! TCTGGTGGATGAATTTTCAG+AGG - chr7_3:76385536-76385555 Msa1125050:five_prime_UTR 40.0%
CTCATGTTTGTAGCTAGATG+TGG - chr7_3:76385735-76385754 Msa1125050:five_prime_UTR 40.0%
! AGTTGGGTTAGGAAACCAAG+GGG + chr7_3:76382340-76382359 None:intergenic 45.0%
! GAAGTTGGGTTAGGAAACCA+AGG + chr7_3:76382342-76382361 None:intergenic 45.0%
GGGGAAGTTAGTTCAAACAG+TGG + chr7_3:76382780-76382799 None:intergenic 45.0%
! GTTCGAGAAGGGATTTTGCA+GGG + chr7_3:76382813-76382832 None:intergenic 45.0%
! TGTTCGAGAAGGGATTTTGC+AGG + chr7_3:76382814-76382833 None:intergenic 45.0%
!! GAAGTGTTGCTGTTCGAGAA+GGG + chr7_3:76382824-76382843 None:intergenic 45.0%
!! AGAAGTGTTGCTGTTCGAGA+AGG + chr7_3:76382825-76382844 None:intergenic 45.0%
TTCTTCTTGAAGCTGCTCTC+AGG - chr7_3:76382841-76382860 Msa1125050:CDS 45.0%
CTATTTCCTGCTTCCGATTC+TGG + chr7_3:76382974-76382993 None:intergenic 45.0%
! CAGGAAAAGCATGTTGGTGA+TGG + chr7_3:76383157-76383176 None:intergenic 45.0%
CTTCAAACAAGTCCTTGCTC+CGG - chr7_3:76383197-76383216 Msa1125050:intron 45.0%
ATTCAAGTCAGGGGAAGAAG+AGG - chr7_3:76383881-76383900 Msa1125050:intron 45.0%
GGAAGAAGAGGAGGAAGATA+AGG - chr7_3:76383893-76383912 Msa1125050:intron 45.0%
!! TGTTTTAGACCCACCATTCG+AGG - chr7_3:76383935-76383954 Msa1125050:CDS 45.0%
CATCATCATCCTCGAATGGT+GGG + chr7_3:76383947-76383966 None:intergenic 45.0%
TCATCATCATCCTCGAATGG+TGG + chr7_3:76383948-76383967 None:intergenic 45.0%
AATCCTTACCAACAAGGCCA+AGG + chr7_3:76384151-76384170 None:intergenic 45.0%
AGGCCAAGTCCACATTGAAT+GGG + chr7_3:76384436-76384455 None:intergenic 45.0%
TAGGCCAAGTCCACATTGAA+TGG + chr7_3:76384437-76384456 None:intergenic 45.0%
TGCTGAGCAGATAAGGAACA+TGG - chr7_3:76385482-76385501 Msa1125050:five_prime_UTR 45.0%
AGCAGATAAGGAACATGGCT+GGG - chr7_3:76385487-76385506 Msa1125050:five_prime_UTR 45.0%
GGATTCTATAGGTTAGGCGG+AGG + chr7_3:76382140-76382159 None:intergenic 50.0%
! TTGGGTTAGGAAACCAAGGG+GGG + chr7_3:76382338-76382357 None:intergenic 50.0%
! GTTGGGTTAGGAAACCAAGG+GGG + chr7_3:76382339-76382358 None:intergenic 50.0%
ACGAAACCAGAATCGGAAGC+AGG - chr7_3:76382965-76382984 Msa1125050:intron 50.0%
GCCGAGTCGAAACATAACTG+AGG - chr7_3:76383244-76383263 Msa1125050:intron 50.0%
! GGTGGGTCTAAAACACAGAC+TGG + chr7_3:76383930-76383949 None:intergenic 50.0%
!! CAACCTTGGCCTTGTTGGTA+AGG - chr7_3:76384145-76384164 Msa1125050:intron 50.0%
CGATGTGAGACCTCGTTGAT+AGG - chr7_3:76384192-76384211 Msa1125050:intron 50.0%
TTCTAACCGTGATGAGCGCA+CGG - chr7_3:76384261-76384280 Msa1125050:intron 50.0%
TCAATGTGGACTTGGCCTAG+TGG - chr7_3:76384438-76384457 Msa1125050:intron 50.0%
GCTGGATCAAGAAGAGGATG+AGG - chr7_3:76385137-76385156 Msa1125050:CDS 50.0%
!! GTGGGATTTGCTGTCTGTCA+TGG + chr7_3:76385272-76385291 None:intergenic 50.0%
ATAGCGACGTGGTGATAGCT+AGG - chr7_3:76385358-76385377 Msa1125050:CDS 50.0%
TCTCTATAGAGGATGGAGGG+TGG - chr7_3:76385451-76385470 Msa1125050:five_prime_UTR 50.0%
GAGCAGATAAGGAACATGGC+TGG - chr7_3:76385486-76385505 Msa1125050:five_prime_UTR 50.0%
GCAGATAAGGAACATGGCTG+GGG - chr7_3:76385488-76385507 Msa1125050:five_prime_UTR 50.0%
GAGATGGGATGGGAGTGAAG+TGG + chr7_3:76382309-76382328 None:intergenic 55.0%
GGGGAGAGATGAGATGGGAT+GGG + chr7_3:76382319-76382338 None:intergenic 55.0%
CAAGGGGGGAGAGATGAGAT+GGG + chr7_3:76382324-76382343 None:intergenic 55.0%
CAAGTCAGGGGAAGAAGAGG+AGG - chr7_3:76383884-76383903 Msa1125050:intron 55.0%
!! AGAGGCAACCTTGGCCTTGT+TGG - chr7_3:76384140-76384159 Msa1125050:intron 55.0%
GGTCTCACATCGACCCTTGT+AGG + chr7_3:76384184-76384203 None:intergenic 55.0%
GGGGGAGAGATGAGATGGGA+TGG + chr7_3:76382320-76382339 None:intergenic 60.0%
CCAAGGGGGGAGAGATGAGA+TGG + chr7_3:76382325-76382344 None:intergenic 60.0%
CCATCTCATCTCTCCCCCCT+TGG - chr7_3:76382322-76382341 Msa1125050:three_prime_UTR 60.0%
TGATGGCCGTGCGCTCATCA+CGG + chr7_3:76384270-76384289 None:intergenic 60.0%
TAACTCCGGGGTGTGGCTGC+CGG + chr7_3:76383219-76383238 None:intergenic 65.0%
ACTCGGCGACGCTAACTCCG+GGG + chr7_3:76383231-76383250 None:intergenic 65.0%
GACTCGGCGACGCTAACTCC+GGG + chr7_3:76383232-76383251 None:intergenic 65.0%
! CGACTCGGCGACGCTAACTC+CGG + chr7_3:76383233-76383252 None:intergenic 65.0%
TTGCTCCGGCAGCCACACCC+CGG - chr7_3:76383211-76383230 Msa1125050:intron 70.0%
GCGACGCTAACTCCGGGGTG+TGG + chr7_3:76383226-76383245 None:intergenic 70.0%
GGGGTGTGGCTGCCGGAGCA+AGG + chr7_3:76383212-76383231 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 76382127 76385817 76382127 ID=Msa1125050;Name=Msa1125050
chr7_3 mRNA 76382127 76385817 76382127 ID=Msa1125050-mRNA-1;Parent=Msa1125050;Name=Msa1125050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=434|1|1|1|1|1|3|245|471
chr7_3 exon 76382127 76382889 76382127 ID=Msa1125050-mRNA-1:exon:20760;Parent=Msa1125050-mRNA-1
chr7_3 exon 76383909 76384101 76383909 ID=Msa1125050-mRNA-1:exon:20759;Parent=Msa1125050-mRNA-1
chr7_3 exon 76384679 76385817 76384679 ID=Msa1125050-mRNA-1:exon:20758;Parent=Msa1125050-mRNA-1
chr7_3 five_prime_UTR 76385384 76385817 76385384 ID=Msa1125050-mRNA-1:five_prime_utr;Parent=Msa1125050-mRNA-1
chr7_3 CDS 76384679 76385383 76384679 ID=Msa1125050-mRNA-1:cds;Parent=Msa1125050-mRNA-1
chr7_3 CDS 76383909 76384101 76383909 ID=Msa1125050-mRNA-1:cds;Parent=Msa1125050-mRNA-1
chr7_3 CDS 76382372 76382889 76382372 ID=Msa1125050-mRNA-1:cds;Parent=Msa1125050-mRNA-1
chr7_3 three_prime_UTR 76382127 76382371 76382127 ID=Msa1125050-mRNA-1:three_prime_utr;Parent=Msa1125050-mRNA-1
Gene Sequence

>Msa1125050

ATGATGGCTCCAAAATACTTACATGAGCTACTAAAAGAAGAGCAAGAACCATTTCTTCTCAACAACTACATTTCACATAAACGTAGCCAAATGAAAATCACTACTTTACAACTCAAGAAAAAACCATTCAACTCAAAATCAAACTTCAACATGAATCTTTGTAAAAACACTTCTTGTTTTTTCTCATTTCCCGAAACACCTGACTTCAAAAAATCACCACTGTTTGAACTAACTTCCCCTGTTAAAAGTCCCTGCAAAATCCCTTCTCGAACAGCAACACTTCTTCTTGAAGCTGCTCTCAGGATCCAGAAACATTCATCATCTTCTTCTTCTTCAAGTTCCAAAAACAAAACAAAAAACAAAGGCTTTGGGCTATTTGGTTCTTTGTTCAAGAAACTAACGCAACGAAACCAGAATCGGAAGCAGGAAATAGAGAATGAATATGTGTCTGTGAGGGAGTTATTGAAGTTGGATTCTTCTGTGAAGGTACGTAGAGAAAAAATGGATTCTCAGAAGAAAAACAACTTGGAACATATGGAAACTTCAAGTAGTGGTTACTCATTTGTTACTGATCATGATGCTTGTGCTTGTCGCCATCACCAACATGCTTTTCCTGAAAGTCCTTTTCGTTTTGCTCTTCAAACAAGTCCTTGCTCCGGCAGCCACACCCCGGAGTTAGCGTCGCCGAGTCGAAACATAACTGAGGACAAAGAAAGTAATGAAGCAGTTGAAAGTATCAATCAATTCAAGTCAGGGGAAGAAGAGGAGGAAGATAAGGAACAGTGTAGTCCAGTCTGTGTTTTAGACCCACCATTCGAGGATGATGATGAAGTACATATAAACGATGATGAAGATGGTGTTGATTTTGATTTAGAGAGCGGCTATGCCATTGTTCAGAGAGCAAGGCAGAAGATATTGTACAAGCTTTGCAGATTTGAGAAACTTGCAGAATTGGATCCATTAGAACTTGAGAAAAGAATGCTGGATCAAGAAGAGGATGAGGATGAAACATATATGGAAGAGGATGATGTTGAAGATGAGGAAAGAGAAGTATCATTTAAAGAAAATGACTTCAAAGAATTGGTTTTTGAAGCTGTGTATCTGTCAATGGTCCATGACAGACAGCAAATCCCACAAGAATTTAAGAAACTGATATCTGATCTAATCGTGGAAGAAGAGAGAGAACTCGATTCTTTAGAAGATAGCGACGTGGTGATAGCTAGGATATTGAAGAGGTTGGAATCATGGAAAGAAGTGGAGTCAAACACAATTGATATGATGATAGAAGAGGATTTCTCTATAGAGGATGGAGGGTGGAAGAAAAATGCTGAGCAGATAAGGAACATGGCTGGGGAGATTGAGCTTGCAATCTTTAGCATTCTGGTGGATGAATTTTCAGAGGAATTAGCATGTTAA
Protein sequence

>Msa1125050

MMAPKYLHELLKEEQEPFLLNNYISHKRSQMKITTLQLKKKPFNSKSNFNMNLCKNTSCFFSFPETPDFKKSPLFELTSPVKSPCKIPSRTATLLLEAALRIQKHSSSSSSSSSKNKTKNKGFGLFGSLFKKLTQRNQNRKQEIENEYVSVRELLKLDSSVKVRREKMDSQKKNNLEHMETSSSGYSFVTDHDACACRHHQHAFPESPFRFALQTSPCSGSHTPELASPSRNITEDKESNEAVESINQFKSGEEEEEDKEQCSPVCVLDPPFEDDDEVHINDDEDGVDFDLESGYAIVQRARQKILYKLCRFEKLAELDPLELEKRMLDQEEDEDETYMEEDDVEDEEREVSFKENDFKELVFEAVYLSMVHDRQQIPQEFKKLISDLIVEEERELDSLEDSDVVIARILKRLESWKEVESNTIDMMIEEDFSIEDGGWKKNAEQIRNMAGEIELAIFSILVDEFSEELAC*