AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1167250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1167250 MtrunA17_Chr7g0265901 92.243 477 24 4 1 465 1 476 0.0 838
Msa1167250 MtrunA17_Chr1g0179151 51.631 521 178 17 1 465 2 504 7.60e-135 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1167250 AT5G03670.1 38.060 536 237 19 1 464 1 513 7.84e-72 236
Msa1167250 AT2G36420.1 33.531 507 209 18 5 463 8 434 1.83e-49 175
Msa1167250 AT5G03670.2 43.850 187 80 9 1 168 1 181 9.66e-31 123

Find 113 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAAGGCTTTGGGCTATT+TGG 0.129747 7_4:+59614086 Msa1167250:CDS
CTATTTCCTGCTTCCGATTC+TGG 0.143562 7_4:-59614139 None:intergenic
AGTAGCTCATGTAAGTATTT+TGG 0.148577 7_4:-59613756 None:intergenic
TAAACCCACATTATATTATT+TGG 0.168834 7_4:-59616869 None:intergenic
TCTAACATGGCATGTATTAT+TGG 0.204106 7_4:+59616809 Msa1167250:three_prime_UTR
AAGAAGATGATGAATGTTTC+TGG 0.233486 7_4:-59614043 None:intergenic
CAGAGGAATTAGCATGTTAA+TGG 0.248136 7_4:+59616724 Msa1167250:CDS
GTCTACTACTACATAATAAA+TGG 0.254280 7_4:-59613449 None:intergenic
GACAGAATAAAATTAAAATT+TGG 0.262186 7_4:-59613393 None:intergenic
GCTTGCAATCTTTAGCATTC+TGG 0.276232 7_4:+59616689 Msa1167250:CDS
GCAAAACGAAAAGGACTTTC+AGG 0.307629 7_4:-59614341 None:intergenic
ATTTGAGAAACTTGCAGAAT+TGG 0.308653 7_4:+59616260 Msa1167250:CDS
AGAAAATGACTTCAAAGAAT+TGG 0.324338 7_4:+59616389 Msa1167250:CDS
AAAATTTGGCAGTAAATTGT+AGG 0.334444 7_4:-59613379 None:intergenic
GGATGAGGATGAAACATATA+TGG 0.353271 7_4:+59616323 Msa1167250:CDS
AGAGGAATTAGCATGTTAAT+GGG 0.362915 7_4:+59616725 Msa1167250:exon
CTAACTAAGTTGTTGTGAAA+TGG 0.368081 7_4:-59613716 None:intergenic
CTAACATGGCATGTATTATT+GGG 0.379285 7_4:+59616810 Msa1167250:three_prime_UTR
TAATTAAGCAAATGAGACTT+AGG 0.405389 7_4:-59616933 None:intergenic
GTGGGATTTGCTGTCTGTCA+TGG 0.410641 7_4:-59616440 None:intergenic
TTAGTTAGTTTATGTGATGA+TGG 0.419692 7_4:+59613731 Msa1167250:exon
AGTATCAATCAATTCAAGTC+AGG 0.420527 7_4:+59615042 Msa1167250:CDS
TCTTCTAATATAAAGAATAA+AGG 0.431130 7_4:-59616780 None:intergenic
AGTTGATTCAAATCTGAAGT+TGG 0.435953 7_4:-59613535 None:intergenic
TGTGAGGGAGTTATTGAAGT+TGG 0.436041 7_4:+59614178 Msa1167250:CDS
GTTGATTCAAATCTGAAGTT+GGG 0.437140 7_4:-59613534 None:intergenic
TAACTCCGGGGTGTGGCTGC+CGG 0.441342 7_4:-59614384 None:intergenic
TATTGAAGAGGTTGGAATCA+TGG 0.442336 7_4:+59616553 Msa1167250:CDS
ATTATTATTGTAAAATAATG+AGG 0.446933 7_4:-59613416 None:intergenic
TCAGTTTCTTAAATTCTTGT+GGG 0.447004 7_4:-59616458 None:intergenic
CTTGTACAGTAGGATTCTAT+AGG 0.450502 7_4:-59613329 None:intergenic
TTCTTCTTGAAGCTGCTCTC+AGG 0.452952 7_4:+59614018 Msa1167250:CDS
TTCAAATCTGAAGTTGGGTT+AGG 0.457886 7_4:-59613529 None:intergenic
ATCAGTTTCTTAAATTCTTG+TGG 0.459352 7_4:-59616459 None:intergenic
ATGTAGTTGTTGAGAAGAAA+TGG 0.459717 7_4:-59613795 None:intergenic
TGAAGCTGTGTATCTGTCAA+TGG 0.460059 7_4:+59616416 Msa1167250:CDS
AGCTAGGATATTGAAGAGGT+TGG 0.467698 7_4:+59616545 Msa1167250:CDS
AGCAGATAAGGAACATGGCT+GGG 0.477023 7_4:+59616658 Msa1167250:CDS
GGGGTGTGGCTGCCGGAGCA+AGG 0.478890 7_4:-59614377 None:intergenic
AGAAGAGGATTTCTCTATAG+AGG 0.482598 7_4:+59616611 Msa1167250:CDS
CGACTCGGCGACGCTAACTC+CGG 0.495649 7_4:-59614398 None:intergenic
ACAGTAGGATTCTATAGGTT+AGG 0.501460 7_4:-59613324 None:intergenic
TAACGCAACGAAACCAGAAT+CGG 0.502950 7_4:+59614126 Msa1167250:CDS
GGAAGAAGAGGAGGAAGATA+AGG 0.504402 7_4:+59615065 Msa1167250:CDS
CAGGAAAAGCATGTTGGTGA+TGG 0.505950 7_4:-59614322 None:intergenic
GTTTGAAGAGCAAAACGAAA+AGG 0.508198 7_4:-59614350 None:intergenic
AATTAAGCAAATGAGACTTA+GGG 0.510109 7_4:-59616932 None:intergenic
TGCAATCTTTAGCATTCTGG+TGG 0.515937 7_4:+59616692 Msa1167250:CDS
ACGAAACCAGAATCGGAAGC+AGG 0.517439 7_4:+59614133 Msa1167250:CDS
GAGCAGATAAGGAACATGGC+TGG 0.519541 7_4:+59616657 Msa1167250:CDS
GAAGTTGGATTCTTCTGTGA+AGG 0.523721 7_4:+59614193 Msa1167250:CDS
GAAGTGTTGCTGTTCGAGAA+GGG 0.524549 7_4:-59613998 None:intergenic
AGAACTTGAGAAAAGAATGC+TGG 0.525436 7_4:+59616290 Msa1167250:CDS
TACTTTCAGTCTTGTACAGT+AGG 0.531514 7_4:-59613339 None:intergenic
GGGGAGAGATGAGATGGGAT+GGG 0.539286 7_4:-59613497 None:intergenic
GGTGGGTCTAAAACACAGAC+TGG 0.539386 7_4:-59615099 None:intergenic
AGTGACCAAATAATATAATG+TGG 0.540524 7_4:+59616864 Msa1167250:three_prime_UTR
GCTGGATCAAGAAGAGGATG+AGG 0.543983 7_4:+59616308 Msa1167250:CDS
ATAGCGACGTGGTGATAGCT+AGG 0.544170 7_4:+59616529 Msa1167250:CDS
ATGGCATGTATTATTGGGAT+AGG 0.545548 7_4:+59616815 Msa1167250:three_prime_UTR
GCGACGCTAACTCCGGGGTG+TGG 0.547073 7_4:-59614391 None:intergenic
TTAAATGGGAGAGAATTTGA+AGG 0.549297 7_4:-59613622 None:intergenic
AGAAGTGTTGCTGTTCGAGA+AGG 0.550922 7_4:-59613999 None:intergenic
GAGGATTTCTCTATAGAGGA+TGG 0.553770 7_4:+59616615 Msa1167250:CDS
ATTCAAGTCAGGGGAAGAAG+AGG 0.558222 7_4:+59615053 Msa1167250:CDS
TCATCATCATCCTCGAATGG+TGG 0.561023 7_4:-59615117 None:intergenic
GAGGAATTAGCATGTTAATG+GGG 0.562269 7_4:+59616726 Msa1167250:exon
GTTGGAATCATGGAAAGAAG+TGG 0.563148 7_4:+59616563 Msa1167250:CDS
CATATAAACGATGATGAAGA+TGG 0.565217 7_4:+59615144 Msa1167250:CDS
GACTTTCAGGAAAAGCATGT+TGG 0.568731 7_4:-59614328 None:intergenic
CATCATCATCCTCGAATGGT+GGG 0.573296 7_4:-59615116 None:intergenic
TTATTATTGTAAAATAATGA+GGG 0.577521 7_4:-59613415 None:intergenic
GTATCAATCAATTCAAGTCA+GGG 0.578564 7_4:+59615043 Msa1167250:CDS
GATTTCTCTATAGAGGATGG+AGG 0.589468 7_4:+59616618 Msa1167250:CDS
AATTGATATGATGATAGAAG+AGG 0.596171 7_4:+59616596 Msa1167250:CDS
AAGAATGCTGGATCAAGAAG+AGG 0.597514 7_4:+59616302 Msa1167250:CDS
CAAGTCAGGGGAAGAAGAGG+AGG 0.599409 7_4:+59615056 Msa1167250:CDS
AGTGTTGCTGTTCGAGAAGG+GGG 0.599413 7_4:-59613996 None:intergenic
CATATAGAAACTTCAAGTAG+TGG 0.602899 7_4:+59614260 Msa1167250:CDS
GTGACCAAATAATATAATGT+GGG 0.603907 7_4:+59616865 Msa1167250:three_prime_UTR
TACAAGACTGAAAGTAGGAA+AGG 0.604462 7_4:+59613346 Msa1167250:five_prime_UTR
CTTCAAACAAGTCCTTGCTC+CGG 0.604522 7_4:+59614365 Msa1167250:CDS
CTCATGTTTGTAGCTAGATG+TGG 0.606774 7_4:+59616904 Msa1167250:three_prime_UTR
TTGCTCCGGCAGCCACACCC+CGG 0.609299 7_4:+59614379 Msa1167250:CDS
ACCTCAGTTATGTTTCGACT+CGG 0.610662 7_4:-59614413 None:intergenic
AAGTGTTGCTGTTCGAGAAG+GGG 0.611165 7_4:-59613997 None:intergenic
GCAGATAAGGAACATGGCTG+GGG 0.618512 7_4:+59616659 Msa1167250:CDS
AGAGAATGATGTTGAAGATG+AGG 0.625938 7_4:+59616347 Msa1167250:CDS
GGATTCTATAGGTTAGGCGG+AGG 0.628968 7_4:-59613318 None:intergenic
ACTTCATCATCATCCTCGAA+TGG 0.634270 7_4:-59615120 None:intergenic
ATAATGTGGGTTTATGTACA+TGG 0.637836 7_4:+59616878 Msa1167250:three_prime_UTR
TGCTGAGCAGATAAGGAACA+TGG 0.637996 7_4:+59616653 Msa1167250:CDS
TACTGTACAAGACTGAAAGT+AGG 0.639842 7_4:+59613341 Msa1167250:five_prime_UTR
TCTCTATAGAGGATGGAGGG+TGG 0.640291 7_4:+59616622 Msa1167250:CDS
TATCAATCAATTCAAGTCAG+GGG 0.642576 7_4:+59615044 Msa1167250:CDS
ACTGATATCTGATCTAATCG+TGG 0.648080 7_4:+59616476 Msa1167250:CDS
GAAGTTGGGTTAGGAAACCA+AGG 0.649026 7_4:-59613520 None:intergenic
ATTTCTCTATAGAGGATGGA+GGG 0.649835 7_4:+59616619 Msa1167250:CDS
GAATGAATATGTGTCTGTGA+GGG 0.651219 7_4:+59614163 Msa1167250:CDS
GTAGGATTCTATAGGTTAGG+CGG 0.651239 7_4:-59613321 None:intergenic
TGATAGCTAGGATATTGAAG+AGG 0.656936 7_4:+59616541 Msa1167250:CDS
AGGAATTAGCATGTTAATGG+GGG 0.657803 7_4:+59616727 Msa1167250:exon
CTGTGACATACTCTGAACAA+TGG 0.660476 7_4:-59615196 None:intergenic
TAGAAGAGTATAGTCTAACA+TGG 0.665718 7_4:+59616796 Msa1167250:three_prime_UTR
TTGGGTTAGGAAACCAAGGG+GGG 0.667246 7_4:-59613516 None:intergenic
AAGTTGGGTTAGGAAACCAA+GGG 0.677084 7_4:-59613519 None:intergenic
GTTGGGTTAGGAAACCAAGG+GGG 0.680940 7_4:-59613517 None:intergenic
GAGATGGGATGGGAGTGAAG+TGG 0.693403 7_4:-59613487 None:intergenic
AGAATGAATATGTGTCTGTG+AGG 0.718238 7_4:+59614162 Msa1167250:CDS
GGGGAAGTTAGTTCAAACAG+TGG 0.724917 7_4:-59613963 None:intergenic
TTCTTTAGAAGATAGCGACG+TGG 0.740114 7_4:+59616518 Msa1167250:CDS
GCCGAGTCGAAACATAACTG+AGG 0.752254 7_4:+59614412 Msa1167250:CDS
AGTTGGGTTAGGAAACCAAG+GGG 0.772580 7_4:-59613518 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTTTTTTATTT+TGG + chr7_4:59614463-59614482 Msa1167250:intron 0.0%
!! TTATTATTGTAAAATAATGA+GGG - chr7_4:59613418-59613437 None:intergenic 10.0%
!! ATTATTATTGTAAAATAATG+AGG - chr7_4:59613419-59613438 None:intergenic 10.0%
!!! TTTTCAATAATAAAAAGTTT+AGG - chr7_4:59615471-59615490 None:intergenic 10.0%
!!! AATTTTATAAAACTTGATAA+GGG - chr7_4:59615771-59615790 None:intergenic 10.0%
!!! AAATTTTATAAAACTTGATA+AGG - chr7_4:59615772-59615791 None:intergenic 10.0%
!! GACAGAATAAAATTAAAATT+TGG - chr7_4:59613396-59613415 None:intergenic 15.0%
!!! TTATTTAATGAGAATTTTTG+TGG + chr7_4:59614980-59614999 Msa1167250:intron 15.0%
!!! ATTTTTGTGGAATTTAATAT+AGG + chr7_4:59614993-59615012 Msa1167250:intron 15.0%
!! TCTTCTAATATAAAGAATAA+AGG - chr7_4:59616783-59616802 None:intergenic 15.0%
!!! TGAAGATGTTTTGTTTAAAT+GGG - chr7_4:59613639-59613658 None:intergenic 20.0%
!!! TTGAAGATGTTTTGTTTAAA+TGG - chr7_4:59613640-59613659 None:intergenic 20.0%
!!! TTTTCGTTTTGAATGTTTAA+TGG + chr7_4:59614509-59614528 Msa1167250:intron 20.0%
!!! TGATTGTTTGTTTGTTTTTT+CGG + chr7_4:59614603-59614622 Msa1167250:intron 20.0%
!!! TTATTGACTCATTTTTTGTA+GGG - chr7_4:59614783-59614802 None:intergenic 20.0%
!!! TTTATTGACTCATTTTTTGT+AGG - chr7_4:59614784-59614803 None:intergenic 20.0%
!! ATACAATCAACAAATTAGAA+TGG - chr7_4:59615242-59615261 None:intergenic 20.0%
!!! ATTCTAATTTGTTGATTGTA+TGG + chr7_4:59615241-59615260 Msa1167250:intron 20.0%
!!! ACTTTTTTGTTTGTTTTGAT+TGG + chr7_4:59615797-59615816 Msa1167250:intron 20.0%
!! TGTAAAATTACTTGTCTTAA+GGG - chr7_4:59615964-59615983 None:intergenic 20.0%
!! ATGTAAAATTACTTGTCTTA+AGG - chr7_4:59615965-59615984 None:intergenic 20.0%
!!! CAAGTAATTTTACATATGTA+CGG + chr7_4:59615970-59615989 Msa1167250:intron 20.0%
!!! ATTTTGAAACTAAGTATGAA+TGG + chr7_4:59616753-59616772 Msa1167250:three_prime_UTR 20.0%
!! TAAACCCACATTATATTATT+TGG - chr7_4:59616872-59616891 None:intergenic 20.0%
! AAAATTTGGCAGTAAATTGT+AGG - chr7_4:59613382-59613401 None:intergenic 25.0%
! GTCTACTACTACATAATAAA+TGG - chr7_4:59613452-59613471 None:intergenic 25.0%
!! TTTTGTATTGTTAGTTAGAG+TGG - chr7_4:59613576-59613595 None:intergenic 25.0%
!! TTAGTTAGTTTATGTGATGA+TGG + chr7_4:59613731-59613750 Msa1167250:exon 25.0%
!! GTAAAGTAGTGATTTTCATT+TGG - chr7_4:59613836-59613855 None:intergenic 25.0%
!!! AAGTTTGATTTTGAGTTGAA+TGG - chr7_4:59613873-59613892 None:intergenic 25.0%
! AAGTACAATCATCAAAATTC+TGG - chr7_4:59614544-59614563 None:intergenic 25.0%
! TTGTACAAAAACACATTAAG+AGG + chr7_4:59615294-59615313 Msa1167250:intron 25.0%
! AAAAGTTTAGGTGAATTTGA+TGG - chr7_4:59615459-59615478 None:intergenic 25.0%
! TGAAAAACAAACACATTTAG+TGG + chr7_4:59615485-59615504 Msa1167250:intron 25.0%
! GAAAAACAAACACATTTAGT+GGG + chr7_4:59615486-59615505 Msa1167250:intron 25.0%
! AAAAACAAACACATTTAGTG+GGG + chr7_4:59615487-59615506 Msa1167250:intron 25.0%
!! CTCCTAAAATGAAATTTTGT+GGG - chr7_4:59615690-59615709 None:intergenic 25.0%
!! TCTCCTAAAATGAAATTTTG+TGG - chr7_4:59615691-59615710 None:intergenic 25.0%
! AGATCTTTCAATCAATATGT+TGG + chr7_4:59615724-59615743 Msa1167250:intron 25.0%
! TGACCTAATTAATACTCATT+GGG + chr7_4:59615868-59615887 Msa1167250:intron 25.0%
! TATCCCAATGAGTATTAATT+AGG - chr7_4:59615874-59615893 None:intergenic 25.0%
!! ATTTTGTGGTACCTTAAATT+TGG + chr7_4:59615895-59615914 Msa1167250:intron 25.0%
! ATTCTCAACAACCAAATTTA+AGG - chr7_4:59615909-59615928 None:intergenic 25.0%
!! ATCTAACCAAGATATTTTAC+AGG + chr7_4:59616204-59616223 Msa1167250:intron 25.0%
!!! ATTCTTTTCTCAAGTTCTAA+TGG - chr7_4:59616288-59616307 None:intergenic 25.0%
! AGAAAATGACTTCAAAGAAT+TGG + chr7_4:59616389-59616408 Msa1167250:CDS 25.0%
! TCAGTTTCTTAAATTCTTGT+GGG - chr7_4:59616461-59616480 None:intergenic 25.0%
! ATCAGTTTCTTAAATTCTTG+TGG - chr7_4:59616462-59616481 None:intergenic 25.0%
! AATTGATATGATGATAGAAG+AGG + chr7_4:59616596-59616615 Msa1167250:CDS 25.0%
! AGTGACCAAATAATATAATG+TGG + chr7_4:59616864-59616883 Msa1167250:three_prime_UTR 25.0%
! GTGACCAAATAATATAATGT+GGG + chr7_4:59616865-59616884 Msa1167250:three_prime_UTR 25.0%
! AATTAAGCAAATGAGACTTA+GGG - chr7_4:59616935-59616954 None:intergenic 25.0%
! TAATTAAGCAAATGAGACTT+AGG - chr7_4:59616936-59616955 None:intergenic 25.0%
! GTTGATTCAAATCTGAAGTT+GGG - chr7_4:59613537-59613556 None:intergenic 30.0%
! AGTTGATTCAAATCTGAAGT+TGG - chr7_4:59613538-59613557 None:intergenic 30.0%
TTAAATGGGAGAGAATTTGA+AGG - chr7_4:59613625-59613644 None:intergenic 30.0%
CTAACTAAGTTGTTGTGAAA+TGG - chr7_4:59613719-59613738 None:intergenic 30.0%
! AGTAGCTCATGTAAGTATTT+TGG - chr7_4:59613759-59613778 None:intergenic 30.0%
ATGTAGTTGTTGAGAAGAAA+TGG - chr7_4:59613798-59613817 None:intergenic 30.0%
!!! TTTTTTGAAGTCAGGTGTTT+CGG - chr7_4:59613940-59613959 None:intergenic 30.0%
AAGAAGATGATGAATGTTTC+TGG - chr7_4:59614046-59614065 None:intergenic 30.0%
! TCTTCAAGTTCCAAAAACAA+AGG + chr7_4:59614071-59614090 Msa1167250:CDS 30.0%
TTCTCAGAAGAAAAACAACT+TGG + chr7_4:59614235-59614254 Msa1167250:CDS 30.0%
CATATAGAAACTTCAAGTAG+TGG + chr7_4:59614260-59614279 Msa1167250:CDS 30.0%
TTTGTAGCAAATGAAACATG+TGG - chr7_4:59614709-59614728 None:intergenic 30.0%
AGTATCAATCAATTCAAGTC+AGG + chr7_4:59615042-59615061 Msa1167250:CDS 30.0%
GTATCAATCAATTCAAGTCA+GGG + chr7_4:59615043-59615062 Msa1167250:CDS 30.0%
TATCAATCAATTCAAGTCAG+GGG + chr7_4:59615044-59615063 Msa1167250:CDS 30.0%
CATATAAACGATGATGAAGA+TGG + chr7_4:59615144-59615163 Msa1167250:CDS 30.0%
!!! GTTGATTTTGATTTAGAGAG+CGG + chr7_4:59615168-59615187 Msa1167250:CDS 30.0%
ATTCAACTCAAATCCTACAA+GGG + chr7_4:59615340-59615359 Msa1167250:intron 30.0%
TTATATATCACCTATCAACG+AGG - chr7_4:59615377-59615396 None:intergenic 30.0%
TGTAGTTGTAAACAAAGAGT+AGG - chr7_4:59615636-59615655 None:intergenic 30.0%
! GACCCACAAAATTTCATTTT+AGG + chr7_4:59615685-59615704 Msa1167250:intron 30.0%
!!! TGTTTTGATTGGCAAATTGT+AGG + chr7_4:59615808-59615827 Msa1167250:intron 30.0%
! TAGGATGTTGGTAAATTAGT+TGG + chr7_4:59615827-59615846 Msa1167250:intron 30.0%
GTGACCTAATTAATACTCAT+TGG + chr7_4:59615867-59615886 Msa1167250:intron 30.0%
! ACTCATTGGGATAAATTTTG+TGG + chr7_4:59615881-59615900 Msa1167250:intron 30.0%
TATGGTACATGTAGCATTAT+AGG - chr7_4:59615941-59615960 None:intergenic 30.0%
AATTACTTGTCTTAAGGGTA+TGG - chr7_4:59615959-59615978 None:intergenic 30.0%
GAATAGAACAGTAAACTGAT+TGG - chr7_4:59616001-59616020 None:intergenic 30.0%
ATTTGAGAAACTTGCAGAAT+TGG + chr7_4:59616260-59616279 Msa1167250:CDS 30.0%
AGAGGAATTAGCATGTTAAT+GGG + chr7_4:59616725-59616744 Msa1167250:exon 30.0%
! TAGAAGAGTATAGTCTAACA+TGG + chr7_4:59616796-59616815 Msa1167250:three_prime_UTR 30.0%
TCTAACATGGCATGTATTAT+TGG + chr7_4:59616809-59616828 Msa1167250:three_prime_UTR 30.0%
CTAACATGGCATGTATTATT+GGG + chr7_4:59616810-59616829 Msa1167250:three_prime_UTR 30.0%
ATAATGTGGGTTTATGTACA+TGG + chr7_4:59616878-59616897 Msa1167250:three_prime_UTR 30.0%
ACAGTAGGATTCTATAGGTT+AGG - chr7_4:59613327-59613346 None:intergenic 35.0%
CTTGTACAGTAGGATTCTAT+AGG - chr7_4:59613332-59613351 None:intergenic 35.0%
! TACTTTCAGTCTTGTACAGT+AGG - chr7_4:59613342-59613361 None:intergenic 35.0%
TACTGTACAAGACTGAAAGT+AGG + chr7_4:59613341-59613360 Msa1167250:five_prime_UTR 35.0%
TACAAGACTGAAAGTAGGAA+AGG + chr7_4:59613346-59613365 Msa1167250:five_prime_UTR 35.0%
! TTCAAATCTGAAGTTGGGTT+AGG - chr7_4:59613532-59613551 None:intergenic 35.0%
!!! TTTTTGAAGTCAGGTGTTTC+GGG - chr7_4:59613939-59613958 None:intergenic 35.0%
!!! AGTGGTGATTTTTTGAAGTC+AGG - chr7_4:59613948-59613967 None:intergenic 35.0%
AGTTCCAAAAACAAAGGCTT+TGG + chr7_4:59614077-59614096 Msa1167250:CDS 35.0%
GTTCCAAAAACAAAGGCTTT+GGG + chr7_4:59614078-59614097 Msa1167250:CDS 35.0%
AGAATGAATATGTGTCTGTG+AGG + chr7_4:59614162-59614181 Msa1167250:CDS 35.0%
GAATGAATATGTGTCTGTGA+GGG + chr7_4:59614163-59614182 Msa1167250:CDS 35.0%
! GAAGGTACGTAGAGAAAAAA+TGG + chr7_4:59614211-59614230 Msa1167250:CDS 35.0%
GTTTGAAGAGCAAAACGAAA+AGG - chr7_4:59614353-59614372 None:intergenic 35.0%
GATTCAACTCAAATCCTACA+AGG + chr7_4:59615339-59615358 Msa1167250:intron 35.0%
ATTGGCAAATTGTAGGATGT+TGG + chr7_4:59615815-59615834 Msa1167250:intron 35.0%
CATACTTTCACTCGACAATA+TGG + chr7_4:59616025-59616044 Msa1167250:intron 35.0%
CTTGCTCCTGTAAAATATCT+TGG - chr7_4:59616213-59616232 None:intergenic 35.0%
! CAAGATATTTTACAGGAGCA+AGG + chr7_4:59616211-59616230 Msa1167250:intron 35.0%
AGAACTTGAGAAAAGAATGC+TGG + chr7_4:59616290-59616309 Msa1167250:CDS 35.0%
GGATGAGGATGAAACATATA+TGG + chr7_4:59616323-59616342 Msa1167250:CDS 35.0%
AGAGAATGATGTTGAAGATG+AGG + chr7_4:59616347-59616366 Msa1167250:CDS 35.0%
ACTGATATCTGATCTAATCG+TGG + chr7_4:59616476-59616495 Msa1167250:CDS 35.0%
TGATAGCTAGGATATTGAAG+AGG + chr7_4:59616541-59616560 Msa1167250:CDS 35.0%
TATTGAAGAGGTTGGAATCA+TGG + chr7_4:59616553-59616572 Msa1167250:CDS 35.0%
AGAAGAGGATTTCTCTATAG+AGG + chr7_4:59616611-59616630 Msa1167250:CDS 35.0%
ATTTCTCTATAGAGGATGGA+GGG + chr7_4:59616619-59616638 Msa1167250:CDS 35.0%
AGAAAAATGCTGAGCAGATA+AGG + chr7_4:59616646-59616665 Msa1167250:CDS 35.0%
CAGAGGAATTAGCATGTTAA+TGG + chr7_4:59616724-59616743 Msa1167250:CDS 35.0%
GAGGAATTAGCATGTTAATG+GGG + chr7_4:59616726-59616745 Msa1167250:exon 35.0%
AGGAATTAGCATGTTAATGG+GGG + chr7_4:59616727-59616746 Msa1167250:exon 35.0%
ATGGCATGTATTATTGGGAT+AGG + chr7_4:59616815-59616834 Msa1167250:three_prime_UTR 35.0%
GTAGGATTCTATAGGTTAGG+CGG - chr7_4:59613324-59613343 None:intergenic 40.0%
! AAGTTGGGTTAGGAAACCAA+GGG - chr7_4:59613522-59613541 None:intergenic 40.0%
!!! TAGCCCAAAGCCTTTGTTTT+TGG - chr7_4:59614084-59614103 None:intergenic 40.0%
!! AACAAAGGCTTTGGGCTATT+TGG + chr7_4:59614086-59614105 Msa1167250:CDS 40.0%
TAACGCAACGAAACCAGAAT+CGG + chr7_4:59614126-59614145 Msa1167250:CDS 40.0%
TGTGAGGGAGTTATTGAAGT+TGG + chr7_4:59614178-59614197 Msa1167250:CDS 40.0%
GAAGTTGGATTCTTCTGTGA+AGG + chr7_4:59614193-59614212 Msa1167250:CDS 40.0%
! GACTTTCAGGAAAAGCATGT+TGG - chr7_4:59614331-59614350 None:intergenic 40.0%
GCAAAACGAAAAGGACTTTC+AGG - chr7_4:59614344-59614363 None:intergenic 40.0%
ACCTCAGTTATGTTTCGACT+CGG - chr7_4:59614416-59614435 None:intergenic 40.0%
CAGAGAGAGAAAGATTTGCA+TGG - chr7_4:59614583-59614602 None:intergenic 40.0%
ACTTCATCATCATCCTCGAA+TGG - chr7_4:59615123-59615142 None:intergenic 40.0%
CTGTGACATACTCTGAACAA+TGG - chr7_4:59615199-59615218 None:intergenic 40.0%
AACACATTAAGAGGCAACCT+TGG + chr7_4:59615303-59615322 Msa1167250:intron 40.0%
GAGTTGAATCCTTACCAACA+AGG - chr7_4:59615329-59615348 None:intergenic 40.0%
ACAGCTGCTCAACATGTAAA+AGG + chr7_4:59615577-59615596 Msa1167250:intron 40.0%
! AAGGCATTTCCCATTCAATG+TGG + chr7_4:59615596-59615615 Msa1167250:intron 40.0%
TTTCCCATTCAATGTGGACT+TGG + chr7_4:59615602-59615621 Msa1167250:intron 40.0%
TAAACAAAGAGTAGGCCACT+AGG - chr7_4:59615628-59615647 None:intergenic 40.0%
AAGAATGCTGGATCAAGAAG+AGG + chr7_4:59616302-59616321 Msa1167250:CDS 40.0%
TGAAGCTGTGTATCTGTCAA+TGG + chr7_4:59616416-59616435 Msa1167250:CDS 40.0%
! TTCTTTAGAAGATAGCGACG+TGG + chr7_4:59616518-59616537 Msa1167250:CDS 40.0%
AGCTAGGATATTGAAGAGGT+TGG + chr7_4:59616545-59616564 Msa1167250:CDS 40.0%
GTTGGAATCATGGAAAGAAG+TGG + chr7_4:59616563-59616582 Msa1167250:CDS 40.0%
GAGGATTTCTCTATAGAGGA+TGG + chr7_4:59616615-59616634 Msa1167250:CDS 40.0%
GATTTCTCTATAGAGGATGG+AGG + chr7_4:59616618-59616637 Msa1167250:CDS 40.0%
GCTTGCAATCTTTAGCATTC+TGG + chr7_4:59616689-59616708 Msa1167250:CDS 40.0%
TGCAATCTTTAGCATTCTGG+TGG + chr7_4:59616692-59616711 Msa1167250:CDS 40.0%
!! TCTGGTGGATGAATTTTCAG+AGG + chr7_4:59616707-59616726 Msa1167250:CDS 40.0%
CTCATGTTTGTAGCTAGATG+TGG + chr7_4:59616904-59616923 Msa1167250:three_prime_UTR 40.0%
! AGTTGGGTTAGGAAACCAAG+GGG - chr7_4:59613521-59613540 None:intergenic 45.0%
! GAAGTTGGGTTAGGAAACCA+AGG - chr7_4:59613523-59613542 None:intergenic 45.0%
GGGGAAGTTAGTTCAAACAG+TGG - chr7_4:59613966-59613985 None:intergenic 45.0%
! AGAAGGGGGACTTTTAACAG+GGG - chr7_4:59613985-59614004 None:intergenic 45.0%
! GAGAAGGGGGACTTTTAACA+GGG - chr7_4:59613986-59614005 None:intergenic 45.0%
! AAGTGTTGCTGTTCGAGAAG+GGG - chr7_4:59614000-59614019 None:intergenic 45.0%
!! GAAGTGTTGCTGTTCGAGAA+GGG - chr7_4:59614001-59614020 None:intergenic 45.0%
!! AGAAGTGTTGCTGTTCGAGA+AGG - chr7_4:59614002-59614021 None:intergenic 45.0%
TTCTTCTTGAAGCTGCTCTC+AGG + chr7_4:59614018-59614037 Msa1167250:CDS 45.0%
CTATTTCCTGCTTCCGATTC+TGG - chr7_4:59614142-59614161 None:intergenic 45.0%
! CAGGAAAAGCATGTTGGTGA+TGG - chr7_4:59614325-59614344 None:intergenic 45.0%
CTTCAAACAAGTCCTTGCTC+CGG + chr7_4:59614365-59614384 Msa1167250:CDS 45.0%
ATTCAAGTCAGGGGAAGAAG+AGG + chr7_4:59615053-59615072 Msa1167250:CDS 45.0%
GGAAGAAGAGGAGGAAGATA+AGG + chr7_4:59615065-59615084 Msa1167250:CDS 45.0%
!! TGTTTTAGACCCACCATTCG+AGG + chr7_4:59615107-59615126 Msa1167250:CDS 45.0%
CATCATCATCCTCGAATGGT+GGG - chr7_4:59615119-59615138 None:intergenic 45.0%
TCATCATCATCCTCGAATGG+TGG - chr7_4:59615120-59615139 None:intergenic 45.0%
AATCCTTACCAACAAGGCCA+AGG - chr7_4:59615323-59615342 None:intergenic 45.0%
AGGCCAAGTCCACATTGAAT+GGG - chr7_4:59615608-59615627 None:intergenic 45.0%
TAGGCCAAGTCCACATTGAA+TGG - chr7_4:59615609-59615628 None:intergenic 45.0%
TGCTGAGCAGATAAGGAACA+TGG + chr7_4:59616653-59616672 Msa1167250:CDS 45.0%
AGCAGATAAGGAACATGGCT+GGG + chr7_4:59616658-59616677 Msa1167250:CDS 45.0%
GGATTCTATAGGTTAGGCGG+AGG - chr7_4:59613321-59613340 None:intergenic 50.0%
! TTGGGTTAGGAAACCAAGGG+GGG - chr7_4:59613519-59613538 None:intergenic 50.0%
! GTTGGGTTAGGAAACCAAGG+GGG - chr7_4:59613520-59613539 None:intergenic 50.0%
! CGAGAAGGGGGACTTTTAAC+AGG - chr7_4:59613987-59614006 None:intergenic 50.0%
! AGTGTTGCTGTTCGAGAAGG+GGG - chr7_4:59613999-59614018 None:intergenic 50.0%
ACGAAACCAGAATCGGAAGC+AGG + chr7_4:59614133-59614152 Msa1167250:CDS 50.0%
GCCGAGTCGAAACATAACTG+AGG + chr7_4:59614412-59614431 Msa1167250:CDS 50.0%
! GGTGGGTCTAAAACACAGAC+TGG - chr7_4:59615102-59615121 None:intergenic 50.0%
!! CAACCTTGGCCTTGTTGGTA+AGG + chr7_4:59615317-59615336 Msa1167250:intron 50.0%
CGATGTGAGACCTCGTTGAT+AGG + chr7_4:59615364-59615383 Msa1167250:intron 50.0%
TTCTAACCGTGATGAGCGCA+CGG + chr7_4:59615433-59615452 Msa1167250:intron 50.0%
TCAATGTGGACTTGGCCTAG+TGG + chr7_4:59615610-59615629 Msa1167250:intron 50.0%
GCTGGATCAAGAAGAGGATG+AGG + chr7_4:59616308-59616327 Msa1167250:CDS 50.0%
!! GTGGGATTTGCTGTCTGTCA+TGG - chr7_4:59616443-59616462 None:intergenic 50.0%
ATAGCGACGTGGTGATAGCT+AGG + chr7_4:59616529-59616548 Msa1167250:CDS 50.0%
TCTCTATAGAGGATGGAGGG+TGG + chr7_4:59616622-59616641 Msa1167250:CDS 50.0%
GAGCAGATAAGGAACATGGC+TGG + chr7_4:59616657-59616676 Msa1167250:CDS 50.0%
GCAGATAAGGAACATGGCTG+GGG + chr7_4:59616659-59616678 Msa1167250:CDS 50.0%
GAGATGGGATGGGAGTGAAG+TGG - chr7_4:59613490-59613509 None:intergenic 55.0%
GGGGAGAGATGAGATGGGAT+GGG - chr7_4:59613500-59613519 None:intergenic 55.0%
CAAGGGGGGAGAGATGAGAT+GGG - chr7_4:59613505-59613524 None:intergenic 55.0%
CAAGTCAGGGGAAGAAGAGG+AGG + chr7_4:59615056-59615075 Msa1167250:CDS 55.0%
!! AGAGGCAACCTTGGCCTTGT+TGG + chr7_4:59615312-59615331 Msa1167250:intron 55.0%
GGTCTCACATCGACCCTTGT+AGG - chr7_4:59615356-59615375 None:intergenic 55.0%
GGGGGAGAGATGAGATGGGA+TGG - chr7_4:59613501-59613520 None:intergenic 60.0%
CCAAGGGGGGAGAGATGAGA+TGG - chr7_4:59613506-59613525 None:intergenic 60.0%
CCATCTCATCTCTCCCCCCT+TGG + chr7_4:59613503-59613522 Msa1167250:five_prime_UTR 60.0%
TGATGGCCGTGCGCTCATCA+CGG - chr7_4:59615442-59615461 None:intergenic 60.0%
TAACTCCGGGGTGTGGCTGC+CGG - chr7_4:59614387-59614406 None:intergenic 65.0%
ACTCGGCGACGCTAACTCCG+GGG - chr7_4:59614399-59614418 None:intergenic 65.0%
GACTCGGCGACGCTAACTCC+GGG - chr7_4:59614400-59614419 None:intergenic 65.0%
! CGACTCGGCGACGCTAACTC+CGG - chr7_4:59614401-59614420 None:intergenic 65.0%
TTGCTCCGGCAGCCACACCC+CGG + chr7_4:59614379-59614398 Msa1167250:CDS 70.0%
GCGACGCTAACTCCGGGGTG+TGG - chr7_4:59614394-59614413 None:intergenic 70.0%
GGGGTGTGGCTGCCGGAGCA+AGG - chr7_4:59614380-59614399 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 59613308 59616986 59613308 ID=Msa1167250;Name=Msa1167250
chr7_4 mRNA 59613308 59616986 59613308 ID=Msa1167250-mRNA-1;Parent=Msa1167250;Name=Msa1167250-mRNA-1;_AED=0.01;_eAED=0.01;_QI=439|1|1|1|1|1|3|243|465
chr7_4 exon 59613308 59614433 59613308 ID=Msa1167250-mRNA-1:exon:18527;Parent=Msa1167250-mRNA-1
chr7_4 exon 59615015 59615207 59615015 ID=Msa1167250-mRNA-1:exon:18528;Parent=Msa1167250-mRNA-1
chr7_4 exon 59616226 59616986 59616226 ID=Msa1167250-mRNA-1:exon:18529;Parent=Msa1167250-mRNA-1
chr7_4 five_prime_UTR 59613308 59613746 59613308 ID=Msa1167250-mRNA-1:five_prime_utr;Parent=Msa1167250-mRNA-1
chr7_4 CDS 59613747 59614433 59613747 ID=Msa1167250-mRNA-1:cds;Parent=Msa1167250-mRNA-1
chr7_4 CDS 59615015 59615207 59615015 ID=Msa1167250-mRNA-1:cds;Parent=Msa1167250-mRNA-1
chr7_4 CDS 59616226 59616743 59616226 ID=Msa1167250-mRNA-1:cds;Parent=Msa1167250-mRNA-1
chr7_4 three_prime_UTR 59616744 59616986 59616744 ID=Msa1167250-mRNA-1:three_prime_utr;Parent=Msa1167250-mRNA-1
Gene Sequence

>Msa1167250

ATGATGGCTCCAAAATACTTACATGAGCTACTAAAAGAAGATCAAGAACCATTTCTTCTCAACAACTACATTTCACATAAACGTAGCCAAATGAAAATCACTACTTTACAACTCAAGAAAAAACCATTCAACTCAAAATCAAACTTCAACATGAATCTTTGTAAAAACACTTCTTGTTTTTTCTCATTTCCCGAAACACCTGACTTCAAAAAATCACCACTGTTTGAACTAACTTCCCCTGTTAAAAGTCCCCCTTCTCGAACAGCAACACTTCTTCTTGAAGCTGCTCTCAGGATCCAGAAACATTCATCATCTTCTTCTTCTTCTTCAAGTTCCAAAAACAAAGGCTTTGGGCTATTTGGTTCTTTGTTCAAGAAACTAACGCAACGAAACCAGAATCGGAAGCAGGAAATAGAGAATGAATATGTGTCTGTGAGGGAGTTATTGAAGTTGGATTCTTCTGTGAAGGTACGTAGAGAAAAAATGGATTCTCAGAAGAAAAACAACTTGGAACATATAGAAACTTCAAGTAGTGGTTACTCATTTGTTACTGATCATGAAGCTTGTGCTTGTCGCCATCACCAACATGCTTTTCCTGAAAGTCCTTTTCGTTTTGCTCTTCAAACAAGTCCTTGCTCCGGCAGCCACACCCCGGAGTTAGCGTCGCCGAGTCGAAACATAACTGAGGACAAAGAAAGTAATGAAGCAGTTGAAAGTATCAATCAATTCAAGTCAGGGGAAGAAGAGGAGGAAGATAAGGAACAGTGTAGTCCAGTCTGTGTTTTAGACCCACCATTCGAGGATGATGATGAAGTACATATAAACGATGATGAAGATGGTGTTGATTTTGATTTAGAGAGCGGCTATGCCATTGTTCAGAGAGCAAGGCAGAAGATATTGTACAAGCTTTGCAGATTTGAGAAACTTGCAGAATTGGATCCATTAGAACTTGAGAAAAGAATGCTGGATCAAGAAGAGGATGAGGATGAAACATATATGGAAGAGAATGATGTTGAAGATGAGGAAAGAGAAGTATCATTTAAAGAAAATGACTTCAAAGAATTGGTTTTTGAAGCTGTGTATCTGTCAATGGTCCATGACAGACAGCAAATCCCACAAGAATTTAAGAAACTGATATCTGATCTAATCGTGGAAGAAGAGAGAGAACTCGATTCTTTAGAAGATAGCGACGTGGTGATAGCTAGGATATTGAAGAGGTTGGAATCATGGAAAGAAGTGGAGTCAAACACAATTGATATGATGATAGAAGAGGATTTCTCTATAGAGGATGGAGGGTGGAAGAAAAATGCTGAGCAGATAAGGAACATGGCTGGGGAGATTGAGCTTGCAATCTTTAGCATTCTGGTGGATGAATTTTCAGAGGAATTAGCATGTTAA
Protein sequence

>Msa1167250

MMAPKYLHELLKEDQEPFLLNNYISHKRSQMKITTLQLKKKPFNSKSNFNMNLCKNTSCFFSFPETPDFKKSPLFELTSPVKSPPSRTATLLLEAALRIQKHSSSSSSSSSSKNKGFGLFGSLFKKLTQRNQNRKQEIENEYVSVRELLKLDSSVKVRREKMDSQKKNNLEHIETSSSGYSFVTDHEACACRHHQHAFPESPFRFALQTSPCSGSHTPELASPSRNITEDKESNEAVESINQFKSGEEEEEDKEQCSPVCVLDPPFEDDDEVHINDDEDGVDFDLESGYAIVQRARQKILYKLCRFEKLAELDPLELEKRMLDQEEDEDETYMEENDVEDEEREVSFKENDFKELVFEAVYLSMVHDRQQIPQEFKKLISDLIVEEERELDSLEDSDVVIARILKRLESWKEVESNTIDMMIEEDFSIEDGGWKKNAEQIRNMAGEIELAIFSILVDEFSEELAC*