AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1080220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1080220 MtrunA17_Chr7g0265901 92.034 477 25 4 1 465 1 476 0.0 835
Msa1080220 MtrunA17_Chr1g0179151 51.440 521 179 17 1 465 2 504 7.33e-134 395
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1080220 AT5G03670.1 37.873 536 238 19 1 464 1 513 7.60e-71 233
Msa1080220 AT2G36420.1 33.333 507 210 18 5 463 8 434 2.51e-48 172
Msa1080220 AT5G03670.2 43.316 187 81 9 1 168 1 181 1.17e-29 120

Find 111 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAAGGCTTTGGGCTATT+TGG 0.129747 7_2:+79527577 Msa1080220:CDS
CTATTTCCTGCTTCCGATTC+TGG 0.143562 7_2:-79527630 None:intergenic
AGTAGCTCATGTAAGTATTT+TGG 0.148577 7_2:-79527247 None:intergenic
TAAACCCACATTATATTATT+TGG 0.168834 7_2:-79530363 None:intergenic
TCTAACATGGAATGTATTAT+TGG 0.229248 7_2:+79530300 Msa1080220:three_prime_UTR
AAGAAGATGATGAATGTTTC+TGG 0.233486 7_2:-79527534 None:intergenic
CAGAGGAATTAGCATGTTAA+TGG 0.248136 7_2:+79530215 Msa1080220:CDS
GTCTACTACTACATAATAAA+TGG 0.254280 7_2:-79526935 None:intergenic
GACAGAATAAAATTAAAATT+TGG 0.262186 7_2:-79526879 None:intergenic
GCTTGCAATCTTTAGCATTC+TGG 0.276232 7_2:+79530180 Msa1080220:CDS
GCAAAACGAAAAGGACTTTC+AGG 0.307629 7_2:-79527832 None:intergenic
ATTTGAGAAACTTGCAGAAT+TGG 0.308653 7_2:+79529751 Msa1080220:CDS
AGAAAATGACTTCAAAGAAT+TGG 0.324338 7_2:+79529880 Msa1080220:CDS
AAAATTTGGCAGTAAATTGT+AGG 0.334444 7_2:-79526865 None:intergenic
GGATGAGGATGAAACATATA+TGG 0.353271 7_2:+79529814 Msa1080220:CDS
AGAGGAATTAGCATGTTAAT+GGG 0.362915 7_2:+79530216 Msa1080220:exon
CTAACTAAGTTGTTGTGAAA+TGG 0.368081 7_2:-79527207 None:intergenic
TAATTAAGCAAATGAGACTT+AGG 0.405389 7_2:-79530427 None:intergenic
CTAACATGGAATGTATTATT+GGG 0.405525 7_2:+79530301 Msa1080220:three_prime_UTR
GTGGGATTTGCTGTCTGTCA+TGG 0.410641 7_2:-79529931 None:intergenic
TTAGTTAGTTTATGTGATGA+TGG 0.419692 7_2:+79527222 Msa1080220:exon
AGTATCAATCAATTCAAGTC+AGG 0.420527 7_2:+79528532 Msa1080220:CDS
TCTTCTAATATAAAGAATAA+AGG 0.431130 7_2:-79530271 None:intergenic
AGTTGATTCAAATCTGAAGT+TGG 0.435953 7_2:-79527021 None:intergenic
TGTGAGGGAGTTATTGAAGT+TGG 0.436041 7_2:+79527669 Msa1080220:CDS
GTTGATTCAAATCTGAAGTT+GGG 0.437140 7_2:-79527020 None:intergenic
TAACTCCGGGGTGTGGCTGC+CGG 0.441342 7_2:-79527875 None:intergenic
TATTGAAGAGGTTGGAATCA+TGG 0.442336 7_2:+79530044 Msa1080220:CDS
ATTATTATTGTAAAATAATG+AGG 0.446933 7_2:-79526902 None:intergenic
TCAGTTTCTTAAATTCTTGT+GGG 0.447004 7_2:-79529949 None:intergenic
CTTGTACAGTAGGATTCTAT+AGG 0.450502 7_2:-79526815 None:intergenic
TTCTTCTTGAAGCTGCTCTC+AGG 0.452952 7_2:+79527509 Msa1080220:CDS
TTCAAATCTGAAGTTGGGTT+AGG 0.457886 7_2:-79527015 None:intergenic
ATCAGTTTCTTAAATTCTTG+TGG 0.459352 7_2:-79529950 None:intergenic
ATGTAGTTGTTGAGAAGAAA+TGG 0.459717 7_2:-79527286 None:intergenic
TGAAGCTGTGTATCTGTCAA+TGG 0.460059 7_2:+79529907 Msa1080220:CDS
AGCTAGGATATTGAAGAGGT+TGG 0.467698 7_2:+79530036 Msa1080220:CDS
AGCAGATAAGGAACATGGCT+GGG 0.477023 7_2:+79530149 Msa1080220:CDS
GGGGTGTGGCTGCCGGAGCA+AGG 0.478890 7_2:-79527868 None:intergenic
AGAAGAGGATTTCTCTATAG+AGG 0.482598 7_2:+79530102 Msa1080220:CDS
CGACTCGGCGACGCTAACTC+CGG 0.495649 7_2:-79527889 None:intergenic
ACAGTAGGATTCTATAGGTT+AGG 0.501460 7_2:-79526810 None:intergenic
TAACGCAACGAAACCAGAAT+CGG 0.502950 7_2:+79527617 Msa1080220:CDS
GGAAGAAGAGGAGGAAGATA+AGG 0.504402 7_2:+79528555 Msa1080220:CDS
CAGGAAAAGCATGTTGGTGA+TGG 0.505950 7_2:-79527813 None:intergenic
GTTTGAAGAGCAAAACGAAA+AGG 0.508198 7_2:-79527841 None:intergenic
AATTAAGCAAATGAGACTTA+GGG 0.510109 7_2:-79530426 None:intergenic
TGCAATCTTTAGCATTCTGG+TGG 0.515937 7_2:+79530183 Msa1080220:CDS
ACGAAACCAGAATCGGAAGC+AGG 0.517439 7_2:+79527624 Msa1080220:CDS
GAGCAGATAAGGAACATGGC+TGG 0.519541 7_2:+79530148 Msa1080220:CDS
ATGGAATGTATTATTGGGAT+AGG 0.523355 7_2:+79530306 Msa1080220:three_prime_UTR
GAAGTTGGATTCTTCTGTGA+AGG 0.523721 7_2:+79527684 Msa1080220:CDS
AGAACTTGAGAAAAGAATGC+TGG 0.525436 7_2:+79529781 Msa1080220:CDS
TACTTTCAGTCTTGTACAGT+AGG 0.531514 7_2:-79526825 None:intergenic
GGGGAGAGATGAGATGGGAT+GGG 0.539286 7_2:-79526983 None:intergenic
GGTGGGTCTAAAACACAGAC+TGG 0.539386 7_2:-79528589 None:intergenic
AGTGTTGCTGTTCGAGAAGA+GGG 0.539964 7_2:-79527487 None:intergenic
AGTGACCAAATAATATAATG+TGG 0.540524 7_2:+79530358 Msa1080220:three_prime_UTR
GCTGGATCAAGAAGAGGATG+AGG 0.543983 7_2:+79529799 Msa1080220:CDS
ATAGCGACGTGGTGATAGCT+AGG 0.544170 7_2:+79530020 Msa1080220:CDS
GCGACGCTAACTCCGGGGTG+TGG 0.547073 7_2:-79527882 None:intergenic
TTAAATGGGAGAGAATTTGA+AGG 0.549297 7_2:-79527113 None:intergenic
GAGGATTTCTCTATAGAGGA+TGG 0.553770 7_2:+79530106 Msa1080220:CDS
ATTCAAGTCAGGGGAAGAAG+AGG 0.558222 7_2:+79528543 Msa1080220:CDS
TCATCATCATCCTCGAATGG+TGG 0.561023 7_2:-79528607 None:intergenic
GAGGAATTAGCATGTTAATG+GGG 0.562269 7_2:+79530217 Msa1080220:exon
GTTGGAATCATGGAAAGAAG+TGG 0.563148 7_2:+79530054 Msa1080220:CDS
CATATAAACGATGATGAAGA+TGG 0.565217 7_2:+79528634 Msa1080220:CDS
GACTTTCAGGAAAAGCATGT+TGG 0.568731 7_2:-79527819 None:intergenic
CATCATCATCCTCGAATGGT+GGG 0.573296 7_2:-79528606 None:intergenic
TTATTATTGTAAAATAATGA+GGG 0.577521 7_2:-79526901 None:intergenic
GTATCAATCAATTCAAGTCA+GGG 0.578564 7_2:+79528533 Msa1080220:CDS
AAGTGTTGCTGTTCGAGAAG+AGG 0.579304 7_2:-79527488 None:intergenic
GATTTCTCTATAGAGGATGG+AGG 0.589468 7_2:+79530109 Msa1080220:CDS
AATTGATATGATGATAGAAG+AGG 0.596171 7_2:+79530087 Msa1080220:CDS
AAGAATGCTGGATCAAGAAG+AGG 0.597514 7_2:+79529793 Msa1080220:CDS
CAAGTCAGGGGAAGAAGAGG+AGG 0.599409 7_2:+79528546 Msa1080220:CDS
CATATAGAAACTTCAAGTAG+TGG 0.602899 7_2:+79527751 Msa1080220:CDS
GTGACCAAATAATATAATGT+GGG 0.603907 7_2:+79530359 Msa1080220:three_prime_UTR
TACAAGACTGAAAGTAGGAA+AGG 0.604462 7_2:+79526832 Msa1080220:five_prime_UTR
CTTCAAACAAGTCCTTGCTC+CGG 0.604522 7_2:+79527856 Msa1080220:CDS
CTCATGTTTGTAGCTAGATG+TGG 0.606774 7_2:+79530398 Msa1080220:three_prime_UTR
TTGCTCCGGCAGCCACACCC+CGG 0.609299 7_2:+79527870 Msa1080220:CDS
ACCTCAGTTATGTTTCGACT+CGG 0.610662 7_2:-79527904 None:intergenic
GCAGATAAGGAACATGGCTG+GGG 0.618512 7_2:+79530150 Msa1080220:CDS
AGAGAATGATGTTGAAGATG+AGG 0.625938 7_2:+79529838 Msa1080220:CDS
GGATTCTATAGGTTAGGCGG+AGG 0.628968 7_2:-79526804 None:intergenic
ACTTCATCATCATCCTCGAA+TGG 0.634270 7_2:-79528610 None:intergenic
TAGAAGAGTATAGTCTAACA+TGG 0.635242 7_2:+79530287 Msa1080220:three_prime_UTR
ATAATGTGGGTTTATGTACA+TGG 0.637836 7_2:+79530372 Msa1080220:three_prime_UTR
TGCTGAGCAGATAAGGAACA+TGG 0.637996 7_2:+79530144 Msa1080220:CDS
TACTGTACAAGACTGAAAGT+AGG 0.639842 7_2:+79526827 Msa1080220:five_prime_UTR
TCTCTATAGAGGATGGAGGG+TGG 0.640291 7_2:+79530113 Msa1080220:CDS
TATCAATCAATTCAAGTCAG+GGG 0.642576 7_2:+79528534 Msa1080220:CDS
ACTGATATCTGATCTAATCG+TGG 0.648080 7_2:+79529967 Msa1080220:CDS
GAAGTTGGGTTAGGAAACCA+AGG 0.649026 7_2:-79527006 None:intergenic
ATTTCTCTATAGAGGATGGA+GGG 0.649835 7_2:+79530110 Msa1080220:CDS
GAATGAATATGTGTCTGTGA+GGG 0.651219 7_2:+79527654 Msa1080220:CDS
GTAGGATTCTATAGGTTAGG+CGG 0.651239 7_2:-79526807 None:intergenic
TGATAGCTAGGATATTGAAG+AGG 0.656936 7_2:+79530032 Msa1080220:CDS
AGGAATTAGCATGTTAATGG+GGG 0.657803 7_2:+79530218 Msa1080220:exon
CTGTGACATACTCTGAACAA+TGG 0.660476 7_2:-79528686 None:intergenic
TTGGGTTAGGAAACCAAGGG+GGG 0.667246 7_2:-79527002 None:intergenic
AAGTTGGGTTAGGAAACCAA+GGG 0.677084 7_2:-79527005 None:intergenic
GTTGGGTTAGGAAACCAAGG+GGG 0.680940 7_2:-79527003 None:intergenic
GAGATGGGATGGGAGTGAAG+TGG 0.693403 7_2:-79526973 None:intergenic
AGAATGAATATGTGTCTGTG+AGG 0.718238 7_2:+79527653 Msa1080220:CDS
GGGGAAGTTAGTTCAAACAG+TGG 0.724917 7_2:-79527454 None:intergenic
TTCTTTAGAAGATAGCGACG+TGG 0.740114 7_2:+79530009 Msa1080220:CDS
GCCGAGTCGAAACATAACTG+AGG 0.752254 7_2:+79527903 Msa1080220:CDS
AGTTGGGTTAGGAAACCAAG+GGG 0.772580 7_2:-79527004 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTTTTTTTTTATTT+TGG + chr7_2:79527953-79527972 Msa1080220:intron 0.0%
!! TTATTATTGTAAAATAATGA+GGG - chr7_2:79526904-79526923 None:intergenic 10.0%
!! ATTATTATTGTAAAATAATG+AGG - chr7_2:79526905-79526924 None:intergenic 10.0%
!!! TTTTCAATAATAAAAAGTTT+AGG - chr7_2:79528961-79528980 None:intergenic 10.0%
!!! AATTTTATAAAACTTGATAA+GGG - chr7_2:79529262-79529281 None:intergenic 10.0%
!!! AAATTTTATAAAACTTGATA+AGG - chr7_2:79529263-79529282 None:intergenic 10.0%
!! GACAGAATAAAATTAAAATT+TGG - chr7_2:79526882-79526901 None:intergenic 15.0%
!!! TTATTTAATGAGAATTTTTG+TGG + chr7_2:79528470-79528489 Msa1080220:intron 15.0%
!!! ATTTTTGTGGAATTTAATAT+AGG + chr7_2:79528483-79528502 Msa1080220:intron 15.0%
!! TCTTCTAATATAAAGAATAA+AGG - chr7_2:79530274-79530293 None:intergenic 15.0%
!!! TGAAGATGTTTTGTTTAAAT+GGG - chr7_2:79527130-79527149 None:intergenic 20.0%
!!! TTGAAGATGTTTTGTTTAAA+TGG - chr7_2:79527131-79527150 None:intergenic 20.0%
!!! TTTTCGTTTTGAATGTTTAA+TGG + chr7_2:79527999-79528018 Msa1080220:intron 20.0%
!!! TGATTGTTTGTTTGTTTTTT+CGG + chr7_2:79528093-79528112 Msa1080220:intron 20.0%
!!! TTATTGACTCATTTTTTGTA+GGG - chr7_2:79528273-79528292 None:intergenic 20.0%
!!! TTTATTGACTCATTTTTTGT+AGG - chr7_2:79528274-79528293 None:intergenic 20.0%
!! ATACAATCAACAAATTAGAA+TGG - chr7_2:79528732-79528751 None:intergenic 20.0%
!!! ATTCTAATTTGTTGATTGTA+TGG + chr7_2:79528731-79528750 Msa1080220:intron 20.0%
!!! ACTTTTTTGTTTGTTTTGAT+TGG + chr7_2:79529288-79529307 Msa1080220:intron 20.0%
!! TGTAAAATTACTTGTCTTAA+GGG - chr7_2:79529455-79529474 None:intergenic 20.0%
!! ATGTAAAATTACTTGTCTTA+AGG - chr7_2:79529456-79529475 None:intergenic 20.0%
!!! CAAGTAATTTTACATATGTA+CGG + chr7_2:79529461-79529480 Msa1080220:intron 20.0%
!!! ATTTTGAAACTAAGTATGAA+TGG + chr7_2:79530244-79530263 Msa1080220:three_prime_UTR 20.0%
!! TAAACCCACATTATATTATT+TGG - chr7_2:79530366-79530385 None:intergenic 20.0%
! AAAATTTGGCAGTAAATTGT+AGG - chr7_2:79526868-79526887 None:intergenic 25.0%
! GTCTACTACTACATAATAAA+TGG - chr7_2:79526938-79526957 None:intergenic 25.0%
!! TTTTGTATTGTTAGTTAGAG+TGG - chr7_2:79527067-79527086 None:intergenic 25.0%
!! TTAGTTAGTTTATGTGATGA+TGG + chr7_2:79527222-79527241 Msa1080220:exon 25.0%
!! GTAAAGTAGTGATTTTCATT+TGG - chr7_2:79527327-79527346 None:intergenic 25.0%
!!! AAGTTTGATTTTGAGTTGAA+TGG - chr7_2:79527364-79527383 None:intergenic 25.0%
! AAGTACAATCATCAAAATTC+TGG - chr7_2:79528034-79528053 None:intergenic 25.0%
! TTGTACAAAAACACATTAAG+AGG + chr7_2:79528784-79528803 Msa1080220:intron 25.0%
! AAAAGTTTAGGTGAATTTGA+TGG - chr7_2:79528949-79528968 None:intergenic 25.0%
! TGAAAAACAAACACATTTAG+TGG + chr7_2:79528975-79528994 Msa1080220:intron 25.0%
! GAAAAACAAACACATTTAGT+GGG + chr7_2:79528976-79528995 Msa1080220:intron 25.0%
! AAAAACAAACACATTTAGTG+GGG + chr7_2:79528977-79528996 Msa1080220:intron 25.0%
!! CTCCTAAAATGAAATTTTGT+GGG - chr7_2:79529181-79529200 None:intergenic 25.0%
!! TCTCCTAAAATGAAATTTTG+TGG - chr7_2:79529182-79529201 None:intergenic 25.0%
! AGATCTTTCAATCAATATGT+TGG + chr7_2:79529215-79529234 Msa1080220:intron 25.0%
! TGACCTAATTAATACTCATT+GGG + chr7_2:79529359-79529378 Msa1080220:intron 25.0%
! TATCCCAATGAGTATTAATT+AGG - chr7_2:79529365-79529384 None:intergenic 25.0%
!! ATTTTGTGGTACCTTAAATT+TGG + chr7_2:79529386-79529405 Msa1080220:intron 25.0%
! ATTCTCAACAACCAAATTTA+AGG - chr7_2:79529400-79529419 None:intergenic 25.0%
!! ATCTAACCAAGATATTTTAC+AGG + chr7_2:79529695-79529714 Msa1080220:intron 25.0%
!!! ATTCTTTTCTCAAGTTCTAA+TGG - chr7_2:79529779-79529798 None:intergenic 25.0%
! AGAAAATGACTTCAAAGAAT+TGG + chr7_2:79529880-79529899 Msa1080220:CDS 25.0%
! TCAGTTTCTTAAATTCTTGT+GGG - chr7_2:79529952-79529971 None:intergenic 25.0%
! ATCAGTTTCTTAAATTCTTG+TGG - chr7_2:79529953-79529972 None:intergenic 25.0%
! AATTGATATGATGATAGAAG+AGG + chr7_2:79530087-79530106 Msa1080220:CDS 25.0%
! TCTAACATGGAATGTATTAT+TGG + chr7_2:79530300-79530319 Msa1080220:three_prime_UTR 25.0%
! CTAACATGGAATGTATTATT+GGG + chr7_2:79530301-79530320 Msa1080220:three_prime_UTR 25.0%
! AGTGACCAAATAATATAATG+TGG + chr7_2:79530358-79530377 Msa1080220:three_prime_UTR 25.0%
! GTGACCAAATAATATAATGT+GGG + chr7_2:79530359-79530378 Msa1080220:three_prime_UTR 25.0%
! AATTAAGCAAATGAGACTTA+GGG - chr7_2:79530429-79530448 None:intergenic 25.0%
! TAATTAAGCAAATGAGACTT+AGG - chr7_2:79530430-79530449 None:intergenic 25.0%
! GTTGATTCAAATCTGAAGTT+GGG - chr7_2:79527023-79527042 None:intergenic 30.0%
! AGTTGATTCAAATCTGAAGT+TGG - chr7_2:79527024-79527043 None:intergenic 30.0%
TTAAATGGGAGAGAATTTGA+AGG - chr7_2:79527116-79527135 None:intergenic 30.0%
CTAACTAAGTTGTTGTGAAA+TGG - chr7_2:79527210-79527229 None:intergenic 30.0%
! AGTAGCTCATGTAAGTATTT+TGG - chr7_2:79527250-79527269 None:intergenic 30.0%
ATGTAGTTGTTGAGAAGAAA+TGG - chr7_2:79527289-79527308 None:intergenic 30.0%
!!! TTTTTTGAAGTCAGGTGTTT+CGG - chr7_2:79527431-79527450 None:intergenic 30.0%
AAGAAGATGATGAATGTTTC+TGG - chr7_2:79527537-79527556 None:intergenic 30.0%
! TCTTCAAGTTCCAAAAACAA+AGG + chr7_2:79527562-79527581 Msa1080220:CDS 30.0%
TTCTCAGAAGAAAAACAACT+TGG + chr7_2:79527726-79527745 Msa1080220:CDS 30.0%
CATATAGAAACTTCAAGTAG+TGG + chr7_2:79527751-79527770 Msa1080220:CDS 30.0%
TTTGTAGCAAATGAAACATG+TGG - chr7_2:79528199-79528218 None:intergenic 30.0%
AGTATCAATCAATTCAAGTC+AGG + chr7_2:79528532-79528551 Msa1080220:CDS 30.0%
GTATCAATCAATTCAAGTCA+GGG + chr7_2:79528533-79528552 Msa1080220:CDS 30.0%
TATCAATCAATTCAAGTCAG+GGG + chr7_2:79528534-79528553 Msa1080220:CDS 30.0%
CATATAAACGATGATGAAGA+TGG + chr7_2:79528634-79528653 Msa1080220:CDS 30.0%
!!! GTTGATTTTGATTTAGAGAG+CGG + chr7_2:79528658-79528677 Msa1080220:CDS 30.0%
ATTCAACTCAAATCCTACAA+GGG + chr7_2:79528830-79528849 Msa1080220:intron 30.0%
TTATATATCACCTATCAACG+AGG - chr7_2:79528867-79528886 None:intergenic 30.0%
TGTAGTTGTAAACAAAGAGT+AGG - chr7_2:79529126-79529145 None:intergenic 30.0%
! GACCCACAAAATTTCATTTT+AGG + chr7_2:79529176-79529195 Msa1080220:intron 30.0%
!!! TGTTTTGATTGGCAAATTGT+AGG + chr7_2:79529299-79529318 Msa1080220:intron 30.0%
! TAGGATGTTGGTAAATTAGT+TGG + chr7_2:79529318-79529337 Msa1080220:intron 30.0%
GTGACCTAATTAATACTCAT+TGG + chr7_2:79529358-79529377 Msa1080220:intron 30.0%
! ACTCATTGGGATAAATTTTG+TGG + chr7_2:79529372-79529391 Msa1080220:intron 30.0%
TATGGTACATGTAGCATTAT+AGG - chr7_2:79529432-79529451 None:intergenic 30.0%
AATTACTTGTCTTAAGGGTA+TGG - chr7_2:79529450-79529469 None:intergenic 30.0%
GAATAGAACAGTAAACTGAT+TGG - chr7_2:79529492-79529511 None:intergenic 30.0%
ATTTGAGAAACTTGCAGAAT+TGG + chr7_2:79529751-79529770 Msa1080220:CDS 30.0%
AGAGGAATTAGCATGTTAAT+GGG + chr7_2:79530216-79530235 Msa1080220:exon 30.0%
! TAGAAGAGTATAGTCTAACA+TGG + chr7_2:79530287-79530306 Msa1080220:three_prime_UTR 30.0%
ATGGAATGTATTATTGGGAT+AGG + chr7_2:79530306-79530325 Msa1080220:three_prime_UTR 30.0%
ATAATGTGGGTTTATGTACA+TGG + chr7_2:79530372-79530391 Msa1080220:three_prime_UTR 30.0%
ACAGTAGGATTCTATAGGTT+AGG - chr7_2:79526813-79526832 None:intergenic 35.0%
CTTGTACAGTAGGATTCTAT+AGG - chr7_2:79526818-79526837 None:intergenic 35.0%
! TACTTTCAGTCTTGTACAGT+AGG - chr7_2:79526828-79526847 None:intergenic 35.0%
TACTGTACAAGACTGAAAGT+AGG + chr7_2:79526827-79526846 Msa1080220:five_prime_UTR 35.0%
TACAAGACTGAAAGTAGGAA+AGG + chr7_2:79526832-79526851 Msa1080220:five_prime_UTR 35.0%
! TTCAAATCTGAAGTTGGGTT+AGG - chr7_2:79527018-79527037 None:intergenic 35.0%
!!! TTTTTGAAGTCAGGTGTTTC+GGG - chr7_2:79527430-79527449 None:intergenic 35.0%
!!! AGTGGTGATTTTTTGAAGTC+AGG - chr7_2:79527439-79527458 None:intergenic 35.0%
AGTTCCAAAAACAAAGGCTT+TGG + chr7_2:79527568-79527587 Msa1080220:CDS 35.0%
GTTCCAAAAACAAAGGCTTT+GGG + chr7_2:79527569-79527588 Msa1080220:CDS 35.0%
AGAATGAATATGTGTCTGTG+AGG + chr7_2:79527653-79527672 Msa1080220:CDS 35.0%
GAATGAATATGTGTCTGTGA+GGG + chr7_2:79527654-79527673 Msa1080220:CDS 35.0%
! GAAGGTACGTAGAGAAAAAA+TGG + chr7_2:79527702-79527721 Msa1080220:CDS 35.0%
GTTTGAAGAGCAAAACGAAA+AGG - chr7_2:79527844-79527863 None:intergenic 35.0%
GATTCAACTCAAATCCTACA+AGG + chr7_2:79528829-79528848 Msa1080220:intron 35.0%
ATTGGCAAATTGTAGGATGT+TGG + chr7_2:79529306-79529325 Msa1080220:intron 35.0%
CATACTTTCACTCGACAATA+TGG + chr7_2:79529516-79529535 Msa1080220:intron 35.0%
CTTGCTCCTGTAAAATATCT+TGG - chr7_2:79529704-79529723 None:intergenic 35.0%
! CAAGATATTTTACAGGAGCA+AGG + chr7_2:79529702-79529721 Msa1080220:intron 35.0%
AGAACTTGAGAAAAGAATGC+TGG + chr7_2:79529781-79529800 Msa1080220:CDS 35.0%
GGATGAGGATGAAACATATA+TGG + chr7_2:79529814-79529833 Msa1080220:CDS 35.0%
AGAGAATGATGTTGAAGATG+AGG + chr7_2:79529838-79529857 Msa1080220:CDS 35.0%
ACTGATATCTGATCTAATCG+TGG + chr7_2:79529967-79529986 Msa1080220:CDS 35.0%
TGATAGCTAGGATATTGAAG+AGG + chr7_2:79530032-79530051 Msa1080220:CDS 35.0%
TATTGAAGAGGTTGGAATCA+TGG + chr7_2:79530044-79530063 Msa1080220:CDS 35.0%
AGAAGAGGATTTCTCTATAG+AGG + chr7_2:79530102-79530121 Msa1080220:CDS 35.0%
ATTTCTCTATAGAGGATGGA+GGG + chr7_2:79530110-79530129 Msa1080220:CDS 35.0%
AGAAAAATGCTGAGCAGATA+AGG + chr7_2:79530137-79530156 Msa1080220:CDS 35.0%
CAGAGGAATTAGCATGTTAA+TGG + chr7_2:79530215-79530234 Msa1080220:CDS 35.0%
GAGGAATTAGCATGTTAATG+GGG + chr7_2:79530217-79530236 Msa1080220:exon 35.0%
AGGAATTAGCATGTTAATGG+GGG + chr7_2:79530218-79530237 Msa1080220:exon 35.0%
GTAGGATTCTATAGGTTAGG+CGG - chr7_2:79526810-79526829 None:intergenic 40.0%
! AAGTTGGGTTAGGAAACCAA+GGG - chr7_2:79527008-79527027 None:intergenic 40.0%
! AGAAGAGGGACTTTTAACAG+GGG - chr7_2:79527476-79527495 None:intergenic 40.0%
! GAGAAGAGGGACTTTTAACA+GGG - chr7_2:79527477-79527496 None:intergenic 40.0%
!!! TAGCCCAAAGCCTTTGTTTT+TGG - chr7_2:79527575-79527594 None:intergenic 40.0%
!! AACAAAGGCTTTGGGCTATT+TGG + chr7_2:79527577-79527596 Msa1080220:CDS 40.0%
TAACGCAACGAAACCAGAAT+CGG + chr7_2:79527617-79527636 Msa1080220:CDS 40.0%
TGTGAGGGAGTTATTGAAGT+TGG + chr7_2:79527669-79527688 Msa1080220:CDS 40.0%
GAAGTTGGATTCTTCTGTGA+AGG + chr7_2:79527684-79527703 Msa1080220:CDS 40.0%
! GACTTTCAGGAAAAGCATGT+TGG - chr7_2:79527822-79527841 None:intergenic 40.0%
GCAAAACGAAAAGGACTTTC+AGG - chr7_2:79527835-79527854 None:intergenic 40.0%
ACCTCAGTTATGTTTCGACT+CGG - chr7_2:79527907-79527926 None:intergenic 40.0%
CAGAGAGAGAAAGATTTGCA+TGG - chr7_2:79528073-79528092 None:intergenic 40.0%
ACTTCATCATCATCCTCGAA+TGG - chr7_2:79528613-79528632 None:intergenic 40.0%
CTGTGACATACTCTGAACAA+TGG - chr7_2:79528689-79528708 None:intergenic 40.0%
AACACATTAAGAGGCAACCT+TGG + chr7_2:79528793-79528812 Msa1080220:intron 40.0%
GAGTTGAATCCTTACCAACA+AGG - chr7_2:79528819-79528838 None:intergenic 40.0%
ACAGCTGCTCAACATGTAAA+AGG + chr7_2:79529067-79529086 Msa1080220:intron 40.0%
! AAGGCATTTCCCATTCAATG+TGG + chr7_2:79529086-79529105 Msa1080220:intron 40.0%
TTTCCCATTCAATGTGGACT+TGG + chr7_2:79529092-79529111 Msa1080220:intron 40.0%
TAAACAAAGAGTAGGCCACT+AGG - chr7_2:79529118-79529137 None:intergenic 40.0%
AAGAATGCTGGATCAAGAAG+AGG + chr7_2:79529793-79529812 Msa1080220:CDS 40.0%
TGAAGCTGTGTATCTGTCAA+TGG + chr7_2:79529907-79529926 Msa1080220:CDS 40.0%
! TTCTTTAGAAGATAGCGACG+TGG + chr7_2:79530009-79530028 Msa1080220:CDS 40.0%
AGCTAGGATATTGAAGAGGT+TGG + chr7_2:79530036-79530055 Msa1080220:CDS 40.0%
GTTGGAATCATGGAAAGAAG+TGG + chr7_2:79530054-79530073 Msa1080220:CDS 40.0%
GAGGATTTCTCTATAGAGGA+TGG + chr7_2:79530106-79530125 Msa1080220:CDS 40.0%
GATTTCTCTATAGAGGATGG+AGG + chr7_2:79530109-79530128 Msa1080220:CDS 40.0%
GCTTGCAATCTTTAGCATTC+TGG + chr7_2:79530180-79530199 Msa1080220:CDS 40.0%
TGCAATCTTTAGCATTCTGG+TGG + chr7_2:79530183-79530202 Msa1080220:CDS 40.0%
!! TCTGGTGGATGAATTTTCAG+AGG + chr7_2:79530198-79530217 Msa1080220:CDS 40.0%
CTCATGTTTGTAGCTAGATG+TGG + chr7_2:79530398-79530417 Msa1080220:three_prime_UTR 40.0%
! AGTTGGGTTAGGAAACCAAG+GGG - chr7_2:79527007-79527026 None:intergenic 45.0%
! GAAGTTGGGTTAGGAAACCA+AGG - chr7_2:79527009-79527028 None:intergenic 45.0%
GGGGAAGTTAGTTCAAACAG+TGG - chr7_2:79527457-79527476 None:intergenic 45.0%
! CGAGAAGAGGGACTTTTAAC+AGG - chr7_2:79527478-79527497 None:intergenic 45.0%
! AGTGTTGCTGTTCGAGAAGA+GGG - chr7_2:79527490-79527509 None:intergenic 45.0%
! AAGTGTTGCTGTTCGAGAAG+AGG - chr7_2:79527491-79527510 None:intergenic 45.0%
TTCTTCTTGAAGCTGCTCTC+AGG + chr7_2:79527509-79527528 Msa1080220:CDS 45.0%
CTATTTCCTGCTTCCGATTC+TGG - chr7_2:79527633-79527652 None:intergenic 45.0%
! CAGGAAAAGCATGTTGGTGA+TGG - chr7_2:79527816-79527835 None:intergenic 45.0%
CTTCAAACAAGTCCTTGCTC+CGG + chr7_2:79527856-79527875 Msa1080220:CDS 45.0%
ATTCAAGTCAGGGGAAGAAG+AGG + chr7_2:79528543-79528562 Msa1080220:CDS 45.0%
GGAAGAAGAGGAGGAAGATA+AGG + chr7_2:79528555-79528574 Msa1080220:CDS 45.0%
!! TGTTTTAGACCCACCATTCG+AGG + chr7_2:79528597-79528616 Msa1080220:CDS 45.0%
CATCATCATCCTCGAATGGT+GGG - chr7_2:79528609-79528628 None:intergenic 45.0%
TCATCATCATCCTCGAATGG+TGG - chr7_2:79528610-79528629 None:intergenic 45.0%
AATCCTTACCAACAAGGCCA+AGG - chr7_2:79528813-79528832 None:intergenic 45.0%
AGGCCAAGTCCACATTGAAT+GGG - chr7_2:79529098-79529117 None:intergenic 45.0%
TAGGCCAAGTCCACATTGAA+TGG - chr7_2:79529099-79529118 None:intergenic 45.0%
TGCTGAGCAGATAAGGAACA+TGG + chr7_2:79530144-79530163 Msa1080220:CDS 45.0%
AGCAGATAAGGAACATGGCT+GGG + chr7_2:79530149-79530168 Msa1080220:CDS 45.0%
GGATTCTATAGGTTAGGCGG+AGG - chr7_2:79526807-79526826 None:intergenic 50.0%
! TTGGGTTAGGAAACCAAGGG+GGG - chr7_2:79527005-79527024 None:intergenic 50.0%
! GTTGGGTTAGGAAACCAAGG+GGG - chr7_2:79527006-79527025 None:intergenic 50.0%
ACGAAACCAGAATCGGAAGC+AGG + chr7_2:79527624-79527643 Msa1080220:CDS 50.0%
GCCGAGTCGAAACATAACTG+AGG + chr7_2:79527903-79527922 Msa1080220:CDS 50.0%
! GGTGGGTCTAAAACACAGAC+TGG - chr7_2:79528592-79528611 None:intergenic 50.0%
!! CAACCTTGGCCTTGTTGGTA+AGG + chr7_2:79528807-79528826 Msa1080220:intron 50.0%
CGATGTGAGACCTCGTTGAT+AGG + chr7_2:79528854-79528873 Msa1080220:intron 50.0%
TTCTAACCGTGATGAGCGCA+CGG + chr7_2:79528923-79528942 Msa1080220:intron 50.0%
TCAATGTGGACTTGGCCTAG+TGG + chr7_2:79529100-79529119 Msa1080220:intron 50.0%
GCTGGATCAAGAAGAGGATG+AGG + chr7_2:79529799-79529818 Msa1080220:CDS 50.0%
!! GTGGGATTTGCTGTCTGTCA+TGG - chr7_2:79529934-79529953 None:intergenic 50.0%
ATAGCGACGTGGTGATAGCT+AGG + chr7_2:79530020-79530039 Msa1080220:CDS 50.0%
TCTCTATAGAGGATGGAGGG+TGG + chr7_2:79530113-79530132 Msa1080220:CDS 50.0%
GAGCAGATAAGGAACATGGC+TGG + chr7_2:79530148-79530167 Msa1080220:CDS 50.0%
GCAGATAAGGAACATGGCTG+GGG + chr7_2:79530150-79530169 Msa1080220:CDS 50.0%
GAGATGGGATGGGAGTGAAG+TGG - chr7_2:79526976-79526995 None:intergenic 55.0%
GGGGAGAGATGAGATGGGAT+GGG - chr7_2:79526986-79527005 None:intergenic 55.0%
CAAGGGGGGAGAGATGAGAT+GGG - chr7_2:79526991-79527010 None:intergenic 55.0%
CAAGTCAGGGGAAGAAGAGG+AGG + chr7_2:79528546-79528565 Msa1080220:CDS 55.0%
!! AGAGGCAACCTTGGCCTTGT+TGG + chr7_2:79528802-79528821 Msa1080220:intron 55.0%
GGTCTCACATCGACCCTTGT+AGG - chr7_2:79528846-79528865 None:intergenic 55.0%
GGGGGAGAGATGAGATGGGA+TGG - chr7_2:79526987-79527006 None:intergenic 60.0%
CCAAGGGGGGAGAGATGAGA+TGG - chr7_2:79526992-79527011 None:intergenic 60.0%
CCATCTCATCTCTCCCCCCT+TGG + chr7_2:79526989-79527008 Msa1080220:five_prime_UTR 60.0%
TGATGGCCGTGCGCTCATCA+CGG - chr7_2:79528932-79528951 None:intergenic 60.0%
TAACTCCGGGGTGTGGCTGC+CGG - chr7_2:79527878-79527897 None:intergenic 65.0%
ACTCGGCGACGCTAACTCCG+GGG - chr7_2:79527890-79527909 None:intergenic 65.0%
GACTCGGCGACGCTAACTCC+GGG - chr7_2:79527891-79527910 None:intergenic 65.0%
! CGACTCGGCGACGCTAACTC+CGG - chr7_2:79527892-79527911 None:intergenic 65.0%
TTGCTCCGGCAGCCACACCC+CGG + chr7_2:79527870-79527889 Msa1080220:CDS 70.0%
GCGACGCTAACTCCGGGGTG+TGG - chr7_2:79527885-79527904 None:intergenic 70.0%
GGGGTGTGGCTGCCGGAGCA+AGG - chr7_2:79527871-79527890 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 79526794 79530480 79526794 ID=Msa1080220;Name=Msa1080220
chr7_2 mRNA 79526794 79530480 79526794 ID=Msa1080220-mRNA-1;Parent=Msa1080220;Name=Msa1080220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=444|1|1|1|1|1|3|246|465
chr7_2 exon 79526794 79527924 79526794 ID=Msa1080220-mRNA-1:exon:20008;Parent=Msa1080220-mRNA-1
chr7_2 exon 79528505 79528697 79528505 ID=Msa1080220-mRNA-1:exon:20009;Parent=Msa1080220-mRNA-1
chr7_2 exon 79529717 79530480 79529717 ID=Msa1080220-mRNA-1:exon:20010;Parent=Msa1080220-mRNA-1
chr7_2 five_prime_UTR 79526794 79527237 79526794 ID=Msa1080220-mRNA-1:five_prime_utr;Parent=Msa1080220-mRNA-1
chr7_2 CDS 79527238 79527924 79527238 ID=Msa1080220-mRNA-1:cds;Parent=Msa1080220-mRNA-1
chr7_2 CDS 79528505 79528697 79528505 ID=Msa1080220-mRNA-1:cds;Parent=Msa1080220-mRNA-1
chr7_2 CDS 79529717 79530234 79529717 ID=Msa1080220-mRNA-1:cds;Parent=Msa1080220-mRNA-1
chr7_2 three_prime_UTR 79530235 79530480 79530235 ID=Msa1080220-mRNA-1:three_prime_utr;Parent=Msa1080220-mRNA-1
Gene Sequence

>Msa1080220

ATGATGGCTCCAAAATACTTACATGAGCTACTAAAAGAAGATCAAGAACCATTTCTTCTCAACAACTACATTTCACATAAACGTAGCCAAATGAAAATCACTACTTTACAACTCAAGAAAAAACCATTCAACTCAAAATCAAACTTCAACATGAATCTTTGTAAAAACACTTCTTGTTTTTTCTCATTTCCCGAAACACCTGACTTCAAAAAATCACCACTGTTTGAACTAACTTCCCCTGTTAAAAGTCCCTCTTCTCGAACAGCAACACTTCTTCTTGAAGCTGCTCTCAGGATCCAGAAACATTCATCATCTTCTTCTTCTTCTTCAAGTTCCAAAAACAAAGGCTTTGGGCTATTTGGTTCTTTGTTCAAGAAACTAACGCAACGAAACCAGAATCGGAAGCAGGAAATAGAGAATGAATATGTGTCTGTGAGGGAGTTATTGAAGTTGGATTCTTCTGTGAAGGTACGTAGAGAAAAAATGGATTCTCAGAAGAAAAACAACTTGGAACATATAGAAACTTCAAGTAGTGGTTACTCATTTGTTACTGATCATGAAGCTTGTGCTTGTCGCCATCACCAACATGCTTTTCCTGAAAGTCCTTTTCGTTTTGCTCTTCAAACAAGTCCTTGCTCCGGCAGCCACACCCCGGAGTTAGCGTCGCCGAGTCGAAACATAACTGAGGACAAAGAAAGTAATGAAGCAGTTGAAAGTATCAATCAATTCAAGTCAGGGGAAGAAGAGGAGGAAGATAAGGAACAGTGTAGTCCAGTCTGTGTTTTAGACCCACCATTCGAGGATGATGATGAAGTACATATAAACGATGATGAAGATGGTGTTGATTTTGATTTAGAGAGCGGCTATGCCATTGTTCAGAGAGCAAGGCAGAAGATATTGTACAAGCTTTGCAGATTTGAGAAACTTGCAGAATTGGATCCATTAGAACTTGAGAAAAGAATGCTGGATCAAGAAGAGGATGAGGATGAAACATATATGGAAGAGAATGATGTTGAAGATGAGGAAAGAGAAGTATCATTTAAAGAAAATGACTTCAAAGAATTGGTTTTTGAAGCTGTGTATCTGTCAATGGTCCATGACAGACAGCAAATCCCACAAGAATTTAAGAAACTGATATCTGATCTAATCGTGGAAGAAGAGAGAGAACTCGATTCTTTAGAAGATAGCGACGTGGTGATAGCTAGGATATTGAAGAGGTTGGAATCATGGAAAGAAGTGGAGTCAAACACAATTGATATGATGATAGAAGAGGATTTCTCTATAGAGGATGGAGGGTGGAAGAAAAATGCTGAGCAGATAAGGAACATGGCTGGGGAGATTGAGCTTGCAATCTTTAGCATTCTGGTGGATGAATTTTCAGAGGAATTAGCATGTTAA
Protein sequence

>Msa1080220

MMAPKYLHELLKEDQEPFLLNNYISHKRSQMKITTLQLKKKPFNSKSNFNMNLCKNTSCFFSFPETPDFKKSPLFELTSPVKSPSSRTATLLLEAALRIQKHSSSSSSSSSSKNKGFGLFGSLFKKLTQRNQNRKQEIENEYVSVRELLKLDSSVKVRREKMDSQKKNNLEHIETSSSGYSFVTDHEACACRHHQHAFPESPFRFALQTSPCSGSHTPELASPSRNITEDKESNEAVESINQFKSGEEEEEDKEQCSPVCVLDPPFEDDDEVHINDDEDGVDFDLESGYAIVQRARQKILYKLCRFEKLAELDPLELEKRMLDQEEDEDETYMEENDVEDEEREVSFKENDFKELVFEAVYLSMVHDRQQIPQEFKKLISDLIVEEERELDSLEDSDVVIARILKRLESWKEVESNTIDMMIEEDFSIEDGGWKKNAEQIRNMAGEIELAIFSILVDEFSEELAC*