AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1225620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225620 MtrunA17_Chr4g0064111 87.963 108 2 1 4 100 934 1041 1.24e-60 199
Msa1225620 MtrunA17_Chr5g0414811 82.407 108 8 1 4 100 134 241 9.59e-60 182
Msa1225620 MtrunA17_Chr3g0090011 85.185 108 5 1 4 100 945 1052 1.87e-58 192
Msa1225620 MtrunA17_Chr5g0414841 85.393 89 2 1 4 81 19 107 2.00e-48 149
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225620 AT5G15920.1 69.725 109 21 2 4 100 944 1052 1.30e-45 156

Find 38 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATCTTCCTTTCATTTAT+TGG 0.074155 8_1:+91098069 None:intergenic
ATCTCATCAACAACTCTAAA+TGG 0.179796 8_1:+91098195 None:intergenic
TGCAGATCTGGTAACAACTT+TGG 0.359641 8_1:+91097653 None:intergenic
GCAGATCTGGTAACAACTTT+GGG 0.363616 8_1:+91097654 None:intergenic
GCCTCGCTATATTGCAGATC+TGG 0.421929 8_1:+91097641 None:intergenic
TACGAGAAATTACCCTTTGA+GGG 0.432664 8_1:+91097575 None:intergenic
GTAGACAAGCTTGGCTGTCT+AGG 0.439784 8_1:-91097229 Msa1225620:three_prime_UTR
TGAATGTAATGAATGGGCCT+TGG 0.469077 8_1:-91097607 Msa1225620:CDS
TTTATACTATTGCTTATACA+AGG 0.479898 8_1:-91097190 Msa1225620:three_prime_UTR
TTAAAGTGAAACAAACACTA+TGG 0.483691 8_1:-91097424 Msa1225620:exon
TTTGAGTAGGCAGCTTATCA+AGG 0.487256 8_1:-91101463 None:intergenic
TCTCATCAACAACTCTAAAT+GGG 0.495363 8_1:+91098196 None:intergenic
TAGTATAAAGAGTAAGAATA+CGG 0.504446 8_1:+91097204 None:intergenic
AATTAATGCGGTAGAGGGTG+TGG 0.506288 8_1:-91097251 Msa1225620:three_prime_UTR
TCTAAGCCGTTGAATTGATC+CGG 0.520956 8_1:-91097281 Msa1225620:three_prime_UTR
TGATAATCAGTCTGGACTGC+TGG 0.523345 8_1:-91097492 Msa1225620:intron
GGTTGTAATTAATGCGGTAG+AGG 0.527796 8_1:-91097257 Msa1225620:three_prime_UTR
GTACGAGAAATTACCCTTTG+AGG 0.530820 8_1:+91097574 None:intergenic
TTATACTATTGCTTATACAA+GGG 0.538609 8_1:-91097189 Msa1225620:three_prime_UTR
TTGGATTGAGCAACCCTCAA+AGG 0.539296 8_1:-91097588 Msa1225620:CDS
CTATTGCTTATACAAGGGTG+AGG 0.547043 8_1:-91097184 Msa1225620:three_prime_UTR
TCTGGACTGCTGGTGATCGA+TGG 0.555627 8_1:-91097482 Msa1225620:CDS
TGGATTGAGCAACCCTCAAA+GGG 0.556056 8_1:-91097587 Msa1225620:intron
GTATATTGAATGTAATGAAT+GGG 0.559079 8_1:-91097613 Msa1225620:CDS
AGTATATTGAATGTAATGAA+TGG 0.565339 8_1:-91097614 Msa1225620:CDS
GATCGATGGAGTATTATCAC+TGG 0.573187 8_1:-91097468 Msa1225620:CDS
TTTGAGGGTTGCTCAATCCA+AGG 0.574514 8_1:+91097590 None:intergenic
ACCGCATTAATTACAACCAC+CGG 0.574778 8_1:+91097262 None:intergenic
GAGGGTGTGGTAGACAAGCT+TGG 0.585047 8_1:-91097238 Msa1225620:three_prime_UTR
GAATGGATCCAATAAATGAA+AGG 0.594716 8_1:-91098077 Msa1225620:CDS
GTTTGTTTCACTTTAACACA+TGG 0.597393 8_1:+91097431 None:intergenic
GTTGTAATTAATGCGGTAGA+GGG 0.600619 8_1:-91097256 Msa1225620:three_prime_UTR
CAACCACCGGATCAATTCAA+CGG 0.612081 8_1:+91097275 None:intergenic
GATACTTGTTAGATACTCAA+CGG 0.618010 8_1:+91097302 None:intergenic
TCCGGTGGTTGTAATTAATG+CGG 0.649174 8_1:-91097263 Msa1225620:three_prime_UTR
GTTGTTGATGAGATCAACCA+AGG 0.650633 8_1:-91098186 Msa1225620:intron
ACCAGATCTGCAATATAGCG+AGG 0.656655 8_1:-91097642 Msa1225620:CDS
AAGCCGTTGAATTGATCCGG+TGG 0.670163 8_1:-91097278 Msa1225620:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAACTAAACTTAAAATA+TGG + chr8_1:91097526-91097545 None:intergenic 10.0%
!!! AAATTTTTTATTATCCATAT+TGG - chr8_1:91097661-91097680 Msa1225620:CDS 10.0%
!!! ATTTTTAAATCTAAAACAAA+CGG + chr8_1:91098639-91098658 None:intergenic 10.0%
!!! AAAAGCATTAATTTTTTTTA+TGG - chr8_1:91099070-91099089 Msa1225620:intron 10.0%
!!! TATATGATATTTTTATGATA+AGG + chr8_1:91099611-91099630 None:intergenic 10.0%
!!! AAAAATATCATATAACTTTT+AGG - chr8_1:91099617-91099636 Msa1225620:intron 10.0%
!!! TAATATTAATTTTTAAACGA+AGG + chr8_1:91100630-91100649 None:intergenic 10.0%
!!! TTTTTTCTTTGTAAAAGATT+TGG - chr8_1:91097315-91097334 Msa1225620:three_prime_UTR 15.0%
!! TACTTTCAATATTCTATATA+AGG - chr8_1:91097474-91097493 Msa1225620:CDS 15.0%
!!! TTTTTAAATCTAAAACAAAC+GGG + chr8_1:91098638-91098657 None:intergenic 15.0%
!! TAGATTTAAAAATATTCAAG+TGG - chr8_1:91098646-91098665 Msa1225620:intron 15.0%
!!! ATATTTTTAGTAAATTCCTT+CGG + chr8_1:91100126-91100145 None:intergenic 15.0%
!! ATTCATTCACTATAAATACA+TGG + chr8_1:91097365-91097384 None:intergenic 20.0%
!! TGTATTTATAGTGAATGAAT+TGG - chr8_1:91097365-91097384 Msa1225620:three_prime_UTR 20.0%
!!! ATAAGGTAGAGTTTTTATTA+TGG - chr8_1:91097491-91097510 Msa1225620:intron 20.0%
!!! TAAGGTAGAGTTTTTATTAT+GGG - chr8_1:91097492-91097511 Msa1225620:intron 20.0%
!! AAAATTTCCAATTCAAGATA+TGG + chr8_1:91097648-91097667 None:intergenic 20.0%
!! AATAATAAACGCTATCTAAA+AGG - chr8_1:91098944-91098963 Msa1225620:intron 20.0%
!! ATAATAAACGCTATCTAAAA+GGG - chr8_1:91098945-91098964 Msa1225620:intron 20.0%
!!! ATGGGAAATATATATAGTTT+TGG - chr8_1:91099168-91099187 Msa1225620:intron 20.0%
!! ATAAAGATCAAAATCTTCTA+TGG - chr8_1:91099326-91099345 Msa1225620:intron 20.0%
!! CAAAATTTGGAAATGAATAA+TGG + chr8_1:91099417-91099436 None:intergenic 20.0%
!! ATTAAGCTTATTAAGTAACA+TGG + chr8_1:91099751-91099770 None:intergenic 20.0%
!! ATCGGAAATCATATATTATT+TGG + chr8_1:91099852-91099871 None:intergenic 20.0%
!! ATAATATATGATTTCCGATA+TGG - chr8_1:91099853-91099872 Msa1225620:intron 20.0%
!! TAATATATGATTTCCGATAT+GGG - chr8_1:91099854-91099873 Msa1225620:intron 20.0%
!! GAGATTAATAATTCAAACTT+AGG + chr8_1:91099968-91099987 None:intergenic 20.0%
!!! TTTTATTCTATCAGAGAAAA+TGG - chr8_1:91100204-91100223 Msa1225620:intron 20.0%
!! AGTATATTGAATGTAATGAA+TGG - chr8_1:91100987-91101006 Msa1225620:intron 20.0%
!! GTATATTGAATGTAATGAAT+GGG - chr8_1:91100988-91101007 Msa1225620:intron 20.0%
!!! AAGATGATAAATGTAGTTTT+CGG + chr8_1:91101052-91101071 None:intergenic 20.0%
!!! ATTTTGTATAGTTAGTTTTG+TGG - chr8_1:91101211-91101230 Msa1225620:intron 20.0%
!! TAGTATAAAGAGTAAGAATA+CGG + chr8_1:91101400-91101419 Msa1225630:CDS 20.0%
!! TTTATACTATTGCTTATACA+AGG - chr8_1:91101411-91101430 Msa1225620:intron 20.0%
!! TTATACTATTGCTTATACAA+GGG - chr8_1:91101412-91101431 Msa1225620:intron 20.0%
! AAGAGAGACATAAGAATATA+GGG - chr8_1:91097210-91097229 Msa1225620:three_prime_UTR 25.0%
! TTGATTAAAACCAACTGATT+TGG - chr8_1:91097283-91097302 Msa1225620:three_prime_UTR 25.0%
!! TTGGAAAAAATGTATTTTCC+TGG - chr8_1:91097334-91097353 Msa1225620:three_prime_UTR 25.0%
! TATAAATACATGGTAATACC+AGG + chr8_1:91097355-91097374 None:intergenic 25.0%
! AAAGTGATGACTATAAAAGA+TGG - chr8_1:91098432-91098451 Msa1225620:intron 25.0%
!! GAATACTCTAAAAATGACTA+AGG + chr8_1:91098511-91098530 None:intergenic 25.0%
!!! AGTCATTTTTAGAGTATTCT+TGG - chr8_1:91098512-91098531 Msa1225620:intron 25.0%
! ACTCTTGTGAATAATAAACA+TGG + chr8_1:91098603-91098622 None:intergenic 25.0%
!! AACTAACTTTCTGTGTTTTT+AGG + chr8_1:91098717-91098736 None:intergenic 25.0%
! TAATAAACGCTATCTAAAAG+GGG - chr8_1:91098946-91098965 Msa1225620:intron 25.0%
!! CATTTTTATCCAAAACAAAC+AGG - chr8_1:91099387-91099406 Msa1225620:intron 25.0%
!! CTAAATATATTTGCTAGAAG+TGG + chr8_1:91099574-91099593 None:intergenic 25.0%
! TAATATATGCAGTACATTGA+TGG + chr8_1:91099715-91099734 None:intergenic 25.0%
!! TCAAACATACTCTTCATTTT+TGG - chr8_1:91099797-91099816 Msa1225620:intron 25.0%
!!! CAAACATACTCTTCATTTTT+GGG - chr8_1:91099798-91099817 Msa1225620:intron 25.0%
! ATCAAATAGTCATTGAGTAA+TGG - chr8_1:91099824-91099843 Msa1225620:intron 25.0%
! TAACTTTGACATTTGTGTAT+TGG + chr8_1:91099909-91099928 None:intergenic 25.0%
! TTAAAGGTTAATTAGCATGT+GGG + chr8_1:91100315-91100334 None:intergenic 25.0%
! TTTAAAGGTTAATTAGCATG+TGG + chr8_1:91100316-91100335 None:intergenic 25.0%
!!! ACCTTTAAATTTGATTTTCC+AGG - chr8_1:91100327-91100346 Msa1225620:intron 25.0%
! TCCTGGAAAATCAAATTTAA+AGG + chr8_1:91100331-91100350 None:intergenic 25.0%
! CCATTCCTGATAAAAAAAAA+GGG + chr8_1:91100510-91100529 None:intergenic 25.0%
! TCCATTCCTGATAAAAAAAA+AGG + chr8_1:91100511-91100530 None:intergenic 25.0%
! AACATCTTCCTTTCATTTAT+TGG + chr8_1:91100535-91100554 None:intergenic 25.0%
! ATAATAATTAGTACTCACTG+TGG + chr8_1:91100595-91100614 None:intergenic 25.0%
!!! TTTTAAGACTTTTGATTAGC+TGG - chr8_1:91100708-91100727 Msa1225620:intron 25.0%
! TAATAAGAGCAATATATGAG+AGG - chr8_1:91100778-91100797 Msa1225620:intron 25.0%
! TTAAAGTGAAACAAACACTA+TGG - chr8_1:91101177-91101196 Msa1225620:intron 25.0%
GAAGAGAGACATAAGAATAT+AGG - chr8_1:91097209-91097228 Msa1225620:three_prime_UTR 30.0%
! ATATAGGGAAGATGATAATG+AGG - chr8_1:91097225-91097244 Msa1225620:three_prime_UTR 30.0%
! AAGATGATAATGAGGAGAAA+TGG - chr8_1:91097233-91097252 Msa1225620:three_prime_UTR 30.0%
! AGATGATAATGAGGAGAAAT+GGG - chr8_1:91097234-91097253 Msa1225620:three_prime_UTR 30.0%
AAAAACTACTCCAAATCAGT+TGG + chr8_1:91097296-91097315 None:intergenic 30.0%
TGTGATTTAGATGTAACCAA+AGG - chr8_1:91097605-91097624 Msa1225620:CDS 30.0%
CGTTGAAAATGAATTTCCTT+TGG + chr8_1:91097624-91097643 None:intergenic 30.0%
AGAATTTGAATTGTGAGCAT+CGG - chr8_1:91097737-91097756 Msa1225620:intron 30.0%
GGAAACTAAATTCCTAAACA+TGG - chr8_1:91097761-91097780 Msa1225620:intron 30.0%
GAATATATGAAAAGACCATG+TGG - chr8_1:91097866-91097885 Msa1225620:intron 30.0%
AATGTATCGTAAAGTTCATG+TGG + chr8_1:91097911-91097930 None:intergenic 30.0%
AAGGTGAAAATACAATGTGT+AGG + chr8_1:91098492-91098511 None:intergenic 30.0%
ACAATAGATGAGATATTCAG+TGG + chr8_1:91098556-91098575 None:intergenic 30.0%
!! TAAGCTCATTTTTGTTGTAG+TGG + chr8_1:91098753-91098772 None:intergenic 30.0%
!!! GCTTATATAGCGCTTTTTTT+TGG - chr8_1:91098768-91098787 Msa1225620:intron 30.0%
TGCTATATAAACCCTACTTT+AGG + chr8_1:91098876-91098895 None:intergenic 30.0%
!! ATTATTGAAAGCGCTATCTA+TGG + chr8_1:91098930-91098949 None:intergenic 30.0%
TTAAAACGCTATGTAACACT+AGG - chr8_1:91099011-91099030 Msa1225620:intron 30.0%
GAAATGTATAGCCACTAATA+TGG - chr8_1:91099273-91099292 Msa1225620:intron 30.0%
TATCCATGTTACCATATTAG+TGG + chr8_1:91099287-91099306 None:intergenic 30.0%
GCATTCTTCATTTATCTTCT+TGG - chr8_1:91099355-91099374 Msa1225620:intron 30.0%
! ATGTTTTCTGCTATATATGC+TGG + chr8_1:91099454-91099473 None:intergenic 30.0%
TAAGAGAAATCCAAGAAGAA+TGG + chr8_1:91099660-91099679 None:intergenic 30.0%
ACATTTGTGTATTGGACATT+GGG + chr8_1:91099901-91099920 None:intergenic 30.0%
AGAAACATAAAGAGTGAAAC+AGG + chr8_1:91100171-91100190 None:intergenic 30.0%
TCTCATCAACAACTCTAAAT+GGG + chr8_1:91100408-91100427 None:intergenic 30.0%
ATCTCATCAACAACTCTAAA+TGG + chr8_1:91100409-91100428 None:intergenic 30.0%
TCTGTAGAGTAAATTCTTTC+TGG - chr8_1:91100446-91100465 Msa1225620:intron 30.0%
AAAAAAAAGGGAGTCAATGA+AGG + chr8_1:91100498-91100517 None:intergenic 30.0%
!!! TGACTCCCTTTTTTTTTATC+AGG - chr8_1:91100502-91100521 Msa1225620:intron 30.0%
!!! CCCTTTTTTTTTATCAGGAA+TGG - chr8_1:91100507-91100526 Msa1225620:intron 30.0%
GAATGGATCCAATAAATGAA+AGG - chr8_1:91100524-91100543 Msa1225620:intron 30.0%
TGCTTGTCATTAGGAAATTA+AGG - chr8_1:91100819-91100838 Msa1225620:intron 30.0%
TTAGAAACAATCAAATGTGG+GGG + chr8_1:91100885-91100904 None:intergenic 30.0%
GTTAGAAACAATCAAATGTG+GGG + chr8_1:91100886-91100905 None:intergenic 30.0%
CGTTAGAAACAATCAAATGT+GGG + chr8_1:91100887-91100906 None:intergenic 30.0%
AGTGACAATTAATCTTATGC+AGG - chr8_1:91100911-91100930 Msa1225620:intron 30.0%
TTTCTTTCTGATAATCAGTC+TGG - chr8_1:91101101-91101120 Msa1225620:intron 30.0%
GTTTGTTTCACTTTAACACA+TGG + chr8_1:91101173-91101192 None:intergenic 30.0%
!! ACTTGCTTTTGTTTCATTGA+TGG - chr8_1:91101264-91101283 Msa1225620:intron 30.0%
!! CTTGCTTTTGTTTCATTGAT+GGG - chr8_1:91101265-91101284 Msa1225620:intron 30.0%
!! TTTTGTTTCATTGATGGGAT+TGG - chr8_1:91101270-91101289 Msa1225620:intron 30.0%
! GATACTTGTTAGATACTCAA+CGG + chr8_1:91101302-91101321 None:intergenic 30.0%
TGTCTCTCTTCAAGTACTTA+TGG + chr8_1:91097200-91097219 None:intergenic 35.0%
GAATGAATTGGCAAAGCAAA+TGG - chr8_1:91097377-91097396 Msa1225620:three_prime_UTR 35.0%
TCAACGACCATATCTTGAAT+TGG - chr8_1:91097638-91097657 Msa1225620:CDS 35.0%
AGGCAAGCATAATTCCAATA+TGG + chr8_1:91097678-91097697 None:intergenic 35.0%
TGAAAATACCAGCCAAATGT+TGG + chr8_1:91097703-91097722 None:intergenic 35.0%
! TGTTGGAGAAAGGATGATTT+TGG - chr8_1:91097791-91097810 Msa1225620:intron 35.0%
ATCGTAAAGTTCATGTGGTA+TGG + chr8_1:91097906-91097925 None:intergenic 35.0%
CATCACTTTCTTGACCTAAT+GGG + chr8_1:91098421-91098440 None:intergenic 35.0%
TCATCACTTTCTTGACCTAA+TGG + chr8_1:91098422-91098441 None:intergenic 35.0%
ATTCACAAGAGTGAGATGTA+TGG - chr8_1:91098611-91098630 Msa1225620:intron 35.0%
TTCACAAGAGTGAGATGTAT+GGG - chr8_1:91098612-91098631 Msa1225620:intron 35.0%
ACACAGAAAGTTAGTTGCAT+AGG - chr8_1:91098721-91098740 Msa1225620:intron 35.0%
CACAGAAAGTTAGTTGCATA+GGG - chr8_1:91098722-91098741 Msa1225620:intron 35.0%
!!! TTTTAGAGCGCTACTGTAAA+TGG - chr8_1:91098804-91098823 Msa1225620:intron 35.0%
! TATAAAGCGCTACCTAAAGT+AGG - chr8_1:91098861-91098880 Msa1225620:intron 35.0%
! ATAAAGCGCTACCTAAAGTA+GGG - chr8_1:91098862-91098881 Msa1225620:intron 35.0%
GTTTAGATAGCGCTTTATAC+AGG - chr8_1:91099033-91099052 Msa1225620:intron 35.0%
!! TTATACAGGAAAAAAGCGCT+AGG - chr8_1:91099047-91099066 Msa1225620:intron 35.0%
TAGCCACTAATATGGTAACA+TGG - chr8_1:91099281-91099300 Msa1225620:intron 35.0%
!! AATGGTTTGCCTGTTTGTTT+TGG + chr8_1:91099399-91099418 None:intergenic 35.0%
! TTTTCTGCTATATATGCTGG+TGG + chr8_1:91099451-91099470 None:intergenic 35.0%
ATATGATTTCCGATATGGGA+TGG - chr8_1:91099858-91099877 Msa1225620:intron 35.0%
ATCATTGATCCATCCCATAT+CGG + chr8_1:91099870-91099889 None:intergenic 35.0%
GACATTTGTGTATTGGACAT+TGG + chr8_1:91099902-91099921 None:intergenic 35.0%
CTAATGACAAGCAAAGCTAT+GGG + chr8_1:91100812-91100831 None:intergenic 35.0%
AAATTAAGGCTGATCTTTGC+CGG - chr8_1:91100833-91100852 Msa1225620:intron 35.0%
GCGTTAGAAACAATCAAATG+TGG + chr8_1:91100888-91100907 None:intergenic 35.0%
TACGAGAAATTACCCTTTGA+GGG + chr8_1:91101029-91101048 None:intergenic 35.0%
GTTGTAATTAATGCGGTAGA+GGG - chr8_1:91101345-91101364 Msa1225620:intron 35.0%
ATCAAGGTTTGGACCCAATA+TGG - chr8_1:91097154-91097173 Msa1225620:three_prime_UTR 40.0%
ACCAAGTACTCCTCCATATT+GGG + chr8_1:91097170-91097189 None:intergenic 40.0%
AACCAAGTACTCCTCCATAT+TGG + chr8_1:91097171-91097190 None:intergenic 40.0%
!! AATATGGAGGAGTACTTGGT+TGG - chr8_1:91097170-91097189 Msa1225620:three_prime_UTR 40.0%
!! ATATGGAGGAGTACTTGGTT+GGG - chr8_1:91097171-91097190 Msa1225620:three_prime_UTR 40.0%
! AATGAGGAGAAATGGGTTTG+AGG - chr8_1:91097241-91097260 Msa1225620:three_prime_UTR 40.0%
TGAATTGGCAAAGCAAATGG+TGG - chr8_1:91097380-91097399 Msa1225620:three_prime_UTR 40.0%
!! ATTTGAATTGTGAGCATCGG+AGG - chr8_1:91097740-91097759 Msa1225620:intron 40.0%
CAACAGTTGCAACCATGTTT+AGG + chr8_1:91097776-91097795 None:intergenic 40.0%
CTAAACATGGTTGCAACTGT+TGG - chr8_1:91097774-91097793 Msa1225620:intron 40.0%
!! CCTAAACACTCAAGTTGAGT+TGG + chr8_1:91097949-91097968 None:intergenic 40.0%
CCAACTCAACTTGAGTGTTT+AGG - chr8_1:91097946-91097965 Msa1225620:intron 40.0%
TACTGTCCAAGTGAAAGTTG+TGG - chr8_1:91098242-91098261 Msa1225620:CDS 40.0%
!! TCCTCATAAGAAGTGTTTCC+TGG - chr8_1:91098265-91098284 Msa1225620:intron 40.0%
ACCAGGAAACACTTCTTATG+AGG + chr8_1:91098269-91098288 None:intergenic 40.0%
GAGTAGCCAATTCTGCTAAT+GGG - chr8_1:91098375-91098394 Msa1225620:intron 40.0%
ATGGCACTTAGAAAGCAAGA+GGG - chr8_1:91098451-91098470 Msa1225620:intron 40.0%
GTTTCAACAAGTAGCTGCTA+TGG - chr8_1:91098676-91098695 Msa1225620:intron 40.0%
!! TTGTCAAAAAGCGCTAGCAA+AGG - chr8_1:91098900-91098919 Msa1225620:intron 40.0%
GGTGGAGAGTGAACAAAATT+TGG + chr8_1:91099430-91099449 None:intergenic 40.0%
TGAAGTGACACCATTCTTCT+TGG - chr8_1:91099647-91099666 Msa1225620:intron 40.0%
! TGAAACAGGAGTGTAAGGTA+TGG + chr8_1:91100157-91100176 None:intergenic 40.0%
! AAGAGTGAAACAGGAGTGTA+AGG + chr8_1:91100162-91100181 None:intergenic 40.0%
TTAATTAGCATGTGGGACAG+GGG + chr8_1:91100308-91100327 None:intergenic 40.0%
GTTAATTAGCATGTGGGACA+GGG + chr8_1:91100309-91100328 None:intergenic 40.0%
! GTTGTTGATGAGATCAACCA+AGG - chr8_1:91100415-91100434 Msa1225620:intron 40.0%
TTGATGAGATCAACCAAGGT+TGG - chr8_1:91100419-91100438 Msa1225620:intron 40.0%
AGTACTCACTGTGGTGTATT+TGG + chr8_1:91100586-91100605 None:intergenic 40.0%
CCTAATGACAAGCAAAGCTA+TGG + chr8_1:91100813-91100832 None:intergenic 40.0%
! CCATAGCTTTGCTTGTCATT+AGG - chr8_1:91100810-91100829 Msa1225620:intron 40.0%
GCAGATCTGGTAACAACTTT+GGG + chr8_1:91100950-91100969 None:intergenic 40.0%
TGCAGATCTGGTAACAACTT+TGG + chr8_1:91100951-91100970 None:intergenic 40.0%
TGAATGTAATGAATGGGCCT+TGG - chr8_1:91100994-91101013 Msa1225620:intron 40.0%
GTACGAGAAATTACCCTTTG+AGG + chr8_1:91101030-91101049 None:intergenic 40.0%
! GATCGATGGAGTATTATCAC+TGG - chr8_1:91101133-91101152 Msa1225620:intron 40.0%
TCTAAGCCGTTGAATTGATC+CGG - chr8_1:91101320-91101339 Msa1225620:intron 40.0%
TCCGGTGGTTGTAATTAATG+CGG - chr8_1:91101338-91101357 Msa1225620:intron 40.0%
ACCGCATTAATTACAACCAC+CGG + chr8_1:91101342-91101361 None:intergenic 40.0%
GGTTGTAATTAATGCGGTAG+AGG - chr8_1:91101344-91101363 Msa1225620:intron 40.0%
CTATTGCTTATACAAGGGTG+AGG - chr8_1:91101417-91101436 Msa1225620:intron 40.0%
AAGGTTTGGACCCAATATGG+AGG - chr8_1:91097157-91097176 Msa1225620:three_prime_UTR 45.0%
!! ACCCAATATGGAGGAGTACT+TGG - chr8_1:91097166-91097185 Msa1225620:three_prime_UTR 45.0%
ATGCTTGCCTTGCCAACATT+TGG - chr8_1:91097688-91097707 Msa1225620:intron 45.0%
ATACCAGCCAAATGTTGGCA+AGG + chr8_1:91097698-91097717 None:intergenic 45.0%
TGGTTGCAACTGTTGGAGAA+AGG - chr8_1:91097781-91097800 Msa1225620:intron 45.0%
GAGTTGGACATGTTGAAGAG+TGG + chr8_1:91097983-91098002 None:intergenic 45.0%
CTGAAGAGTAGAGTTGAGCT+AGG + chr8_1:91098192-91098211 None:intergenic 45.0%
CTAGCTCAACTCTACTCTTC+AGG - chr8_1:91098190-91098209 Msa1225620:CDS 45.0%
ACAGTAACGTGAGGAAGAAG+TGG + chr8_1:91098228-91098247 None:intergenic 45.0%
TTCACTTGGACAGTAACGTG+AGG + chr8_1:91098237-91098256 None:intergenic 45.0%
TGAGGACCACAACTTTCACT+TGG + chr8_1:91098251-91098270 None:intergenic 45.0%
TCTCATCCGTACATGACACA+TGG + chr8_1:91098343-91098362 None:intergenic 45.0%
GGAGTAGCCAATTCTGCTAA+TGG - chr8_1:91098374-91098393 Msa1225620:intron 45.0%
GATGGCACTTAGAAAGCAAG+AGG - chr8_1:91098450-91098469 Msa1225620:intron 45.0%
GTATTCTTGGAGCTCCTCAT+AGG - chr8_1:91098525-91098544 Msa1225620:intron 45.0%
ATTCAGTGGAGCTACCTATG+AGG + chr8_1:91098542-91098561 None:intergenic 45.0%
!! ACTGTAAATGGCGCTGGTAA+AGG - chr8_1:91098816-91098835 Msa1225620:intron 45.0%
TCTGCTATATATGCTGGTGG+TGG + chr8_1:91099448-91099467 None:intergenic 45.0%
! AACAGGAGTGTAAGGTATGG+AGG + chr8_1:91100154-91100173 None:intergenic 45.0%
! GTACCCTCCAGATTGATGAT+GGG + chr8_1:91100250-91100269 None:intergenic 45.0%
AGTACCCTCCAGATTGATGA+TGG + chr8_1:91100251-91100270 None:intergenic 45.0%
GGTTAATTAGCATGTGGGAC+AGG + chr8_1:91100310-91100329 None:intergenic 45.0%
TAGTTGAAACTGATCGCTCC+TGG + chr8_1:91100348-91100367 None:intergenic 45.0%
CTCTACAGACTAACCAACCT+TGG + chr8_1:91100435-91100454 None:intergenic 45.0%
! GGTCTCTTAAGTGCTGTCTA+CGG + chr8_1:91100477-91100496 None:intergenic 45.0%
TTAGCTGGAAGAGACACACT+CGG - chr8_1:91100723-91100742 Msa1225620:intron 45.0%
GAACAAAGCACATTAGCGAC+TGG + chr8_1:91100863-91100882 None:intergenic 45.0%
ACCAGATCTGCAATATAGCG+AGG - chr8_1:91100959-91100978 Msa1225620:intron 45.0%
TTTGAGGGTTGCTCAATCCA+AGG + chr8_1:91101014-91101033 None:intergenic 45.0%
TTGGATTGAGCAACCCTCAA+AGG - chr8_1:91101013-91101032 Msa1225620:intron 45.0%
TGGATTGAGCAACCCTCAAA+GGG - chr8_1:91101014-91101033 Msa1225620:intron 45.0%
TGATAATCAGTCTGGACTGC+TGG - chr8_1:91101109-91101128 Msa1225620:intron 45.0%
CAACCACCGGATCAATTCAA+CGG + chr8_1:91101329-91101348 None:intergenic 45.0%
AATTAATGCGGTAGAGGGTG+TGG - chr8_1:91101350-91101369 Msa1225620:intron 45.0%
!!! ATAATATATAACTTTTTTTT+TGG - chr8_1:91097438-91097457 Msa1225620:exon 5.0%
!!! ATATATATATAATTTTTCTA+TGG - chr8_1:91099149-91099168 Msa1225620:intron 5.0%
!!! TATATATATAATTTTTCTAT+GGG - chr8_1:91099150-91099169 Msa1225620:intron 5.0%
TTGCCTTGCCAACATTTGGC+TGG - chr8_1:91097692-91097711 Msa1225620:intron 50.0%
GACCATGTGGACTCGTATAG+AGG - chr8_1:91097879-91097898 Msa1225620:intron 50.0%
GTCCTCTATACGAGTCCACA+TGG + chr8_1:91097884-91097903 None:intergenic 50.0%
! GCTGAAGGGTATAGCTGAGT+TGG + chr8_1:91097999-91098018 None:intergenic 50.0%
GTTGAGCTGGACATGCTGAA+GGG + chr8_1:91098013-91098032 None:intergenic 50.0%
AGTTGAGCTGGACATGCTGA+AGG + chr8_1:91098014-91098033 None:intergenic 50.0%
GCTGAGCTCAACATGCTGAA+GGG + chr8_1:91098067-91098086 None:intergenic 50.0%
AGCTGAGCTCAACATGCTGA+AGG + chr8_1:91098068-91098087 None:intergenic 50.0%
GTTGAGCTAGGCATGCTGAA+GGG + chr8_1:91098180-91098199 None:intergenic 50.0%
AGTTGAGCTAGGCATGCTGA+AGG + chr8_1:91098181-91098200 None:intergenic 50.0%
CCAGCTCCATGTGTCATGTA+CGG - chr8_1:91098334-91098353 Msa1225620:intron 50.0%
ACGGATGAGATGCACAGAGA+AGG - chr8_1:91098353-91098372 Msa1225620:intron 50.0%
TCCAGGCCCATTAGCAGAAT+TGG + chr8_1:91098384-91098403 None:intergenic 50.0%
CATCCATAGAGAGGCCCATT+AGG - chr8_1:91098404-91098423 Msa1225620:intron 50.0%
! TGACCTAATGGGCCTCTCTA+TGG + chr8_1:91098410-91098429 None:intergenic 50.0%
CTCAGTGCCCATCATCAATC+TGG - chr8_1:91100240-91100259 Msa1225620:intron 50.0%
AGTGCCCATCATCAATCTGG+AGG - chr8_1:91100243-91100262 Msa1225620:intron 50.0%
GTGCCCATCATCAATCTGGA+GGG - chr8_1:91100244-91100263 Msa1225620:intron 50.0%
!! CAATCTGGAGGGTACTACAG+AGG - chr8_1:91100255-91100274 Msa1225620:intron 50.0%
CACATTAGCGACTGGCAAAC+CGG + chr8_1:91100855-91100874 None:intergenic 50.0%
GCCTCGCTATATTGCAGATC+TGG + chr8_1:91100963-91100982 None:intergenic 50.0%
AAGCCGTTGAATTGATCCGG+TGG - chr8_1:91101323-91101342 Msa1225620:intron 50.0%
GTAGACAAGCTTGGCTGTCT+AGG - chr8_1:91101372-91101391 Msa1225620:intron 50.0%
GCTGAGCTGAGCATGCTGAA+GGG + chr8_1:91098040-91098059 None:intergenic 55.0%
AGCTGAGCTGAGCATGCTGA+AGG + chr8_1:91098041-91098060 None:intergenic 55.0%
CTTACGTCACTTGTCCGTGC+AGG + chr8_1:91098303-91098322 None:intergenic 55.0%
CCGTACATGACACATGGAGC+TGG + chr8_1:91098337-91098356 None:intergenic 55.0%
! GCCAATTCTGCTAATGGGCC+TGG - chr8_1:91098380-91098399 Msa1225620:intron 55.0%
AGCGCTACTGTAAATGGCGC+TGG - chr8_1:91098810-91098829 Msa1225620:intron 55.0%
TCTGGACTGCTGGTGATCGA+TGG - chr8_1:91101119-91101138 Msa1225620:intron 55.0%
! GAGGGTGTGGTAGACAAGCT+TGG - chr8_1:91101363-91101382 Msa1225620:intron 55.0%
! GCTGAAGGGACGAGTTGAGC+TGG + chr8_1:91098026-91098045 None:intergenic 60.0%
CTCAGCTCGTCCCTTCAGCA+TGG - chr8_1:91098054-91098073 Msa1225620:intron 60.0%
GCTGAGCTGGCCATGCTGAA+GGG + chr8_1:91098099-91098118 None:intergenic 60.0%
AGCTGAGCTGGCCATGCTGA+AGG + chr8_1:91098100-91098119 None:intergenic 60.0%
TTCAGCATGGCCAGCTCAGC+TGG - chr8_1:91098099-91098118 Msa1225620:intron 60.0%
GAGCTGAGCTGAGCATGCTG+AGG + chr8_1:91098042-91098061 None:intergenic 60.0%
GCTGAGCTGGGCATGCTGAA+GGG + chr8_1:91098153-91098172 None:intergenic 60.0%
AGCTGAGCTGGGCATGCTGA+AGG + chr8_1:91098154-91098173 None:intergenic 60.0%
CTGAAGGGTCGAGCTGAGCT+GGG + chr8_1:91098138-91098157 None:intergenic 60.0%
ACTTGTCCGTGCAGGATGCG+TGG + chr8_1:91098295-91098314 None:intergenic 60.0%
GGGCCTGGACATCCATAGAG+AGG - chr8_1:91098395-91098414 Msa1225620:intron 60.0%
GGGCCTCTCTATGGATGTCC+AGG + chr8_1:91098401-91098420 None:intergenic 60.0%
GCTGAGGAGTCCAGCTGAGC+TGG + chr8_1:91098112-91098131 None:intergenic 65.0%
GCTGAAGGGTCGAGCTGAGC+TGG + chr8_1:91098139-91098158 None:intergenic 65.0%
TGCAGGATGCGTGGCGCACC+AGG + chr8_1:91098286-91098305 None:intergenic 70.0%
GGTGCGCCACGCATCCTGCA+CGG - chr8_1:91098286-91098305 Msa1225620:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 91097151 91101472 91097151 ID=Msa1225620;Name=Msa1225620
chr8_1 mRNA 91097151 91101472 91097151 ID=Msa1225620-mRNA-1;Parent=Msa1225620;Name=Msa1225620-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.5|0.4|0.8|0.75|0.8|5|292|100
chr8_1 exon 91097151 91097504 91097151 ID=Msa1225620-mRNA-1:exon:33279;Parent=Msa1225620-mRNA-1
chr8_1 exon 91097588 91097690 91097588 ID=Msa1225620-mRNA-1:exon:33278;Parent=Msa1225620-mRNA-1
chr8_1 exon 91098059 91098099 91098059 ID=Msa1225620-mRNA-1:exon:33277;Parent=Msa1225620-mRNA-1
chr8_1 exon 91098187 91098274 91098187 ID=Msa1225620-mRNA-1:exon:33276;Parent=Msa1225620-mRNA-1
chr8_1 exon 91101464 91101472 91101464 ID=Msa1225620-mRNA-1:exon:33275;Parent=Msa1225620-mRNA-1
chr8_1 CDS 91101464 91101472 91101464 ID=Msa1225620-mRNA-1:cds;Parent=Msa1225620-mRNA-1
chr8_1 CDS 91098187 91098274 91098187 ID=Msa1225620-mRNA-1:cds;Parent=Msa1225620-mRNA-1
chr8_1 CDS 91098059 91098099 91098059 ID=Msa1225620-mRNA-1:cds;Parent=Msa1225620-mRNA-1
chr8_1 CDS 91097588 91097690 91097588 ID=Msa1225620-mRNA-1:cds;Parent=Msa1225620-mRNA-1
chr8_1 CDS 91097443 91097504 91097443 ID=Msa1225620-mRNA-1:cds;Parent=Msa1225620-mRNA-1
chr8_1 three_prime_UTR 91097151 91097442 91097151 ID=Msa1225620-mRNA-1:three_prime_utr;Parent=Msa1225620-mRNA-1
Gene Sequence

>Msa1225620

CTTATCAAGGAGCGATCAGTTTCAACTATTGTTTATCTTGTTTCTCTTCAAGATCTTACAAACTGCCCATTTAGAGTTGTTGATGAGATCAACCAAGGAATGGATCCAATAAATGAAAGGAAGATGTTTCAGCAACTAGTGTTTCTTACTTACCCAAAGTTGTTACCAGATCTGCAATATAGCGAGGCATGCAGTATATTGAATGTAATGAATGGGCCTTGGATTGAGCAACCCTCAAAGGTCTGGACTGCTGGTGATCGATGGAGTATTATCACTGGACATGTTGAAGAGACCATGTGTTAA
Protein sequence

>Msa1225620

LIKERSVSTIVYLVSLQDLTNCPFRVVDEINQGMDPINERKMFQQLVFLTYPKLLPDLQYSEACSILNVMNGPWIEQPSKVWTAGDRWSIITGHVEETMC*