AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1447900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1447900 MtrunA17_Chr7g0276531 84.528 265 20 6 3 255 1 256 4.20e-144 404
Msa1447900 MtrunA17_Chr8g0346011 67.010 97 32 0 12 108 25 121 1.07e-43 149
Msa1447900 MtrunA17_Chr5g0426311 74.419 86 22 0 16 101 8 93 1.86e-43 148
Msa1447900 MtrunA17_Chr3g0144311 50.303 165 72 4 16 173 21 182 4.20e-43 147
Msa1447900 MtrunA17_Chr4g0017401 66.667 93 31 0 17 109 9 101 2.44e-41 142
Msa1447900 MtrunA17_Chr3g0127751 50.382 131 55 2 12 132 14 144 1.22e-37 132
Msa1447900 MtrunA17_Chr4g0045921 48.810 84 43 0 20 103 72 155 2.06e-19 86.3
Msa1447900 MtrunA17_Chr4g0045921 32.584 89 59 1 19 107 158 245 8.67e-12 64.3
Msa1447900 MtrunA17_Chr7g0255011 44.048 84 47 0 19 102 122 205 5.03e-18 82.8
Msa1447900 MtrunA17_Chr7g0266751 37.391 115 69 2 10 121 16 130 5.58e-18 78.2
Msa1447900 MtrunA17_Chr2g0290191 47.059 68 35 1 25 92 5 71 1.07e-17 82.0
Msa1447900 MtrunA17_Chr8g0360551 44.578 83 46 0 20 102 69 151 1.25e-17 81.6
Msa1447900 MtrunA17_Chr8g0360551 37.363 91 57 0 9 99 150 240 3.07e-13 68.9
Msa1447900 MtrunA17_Chr2g0278661 48.649 74 37 1 20 92 3 76 1.63e-17 79.3
Msa1447900 MtrunA17_Chr3g0101061 42.105 76 43 1 17 92 33 107 6.38e-17 79.7
Msa1447900 MtrunA17_Chr4g0061451 43.529 85 44 2 24 107 42 123 1.05e-16 77.4
Msa1447900 MtrunA17_Chr7g0219021 43.210 81 45 1 25 104 41 121 2.68e-16 76.3
Msa1447900 MtrunA17_Chr3g0121631 43.478 69 39 0 24 92 36 104 3.66e-16 73.2
Msa1447900 MtrunA17_Chr3g0086861 42.857 91 48 2 18 107 38 125 1.04e-15 76.3
Msa1447900 MtrunA17_Chr4g0035051 49.153 59 30 0 25 83 9 67 1.07e-15 71.2
Msa1447900 MtrunA17_Chr5g0440831 39.744 78 47 0 25 102 166 243 3.06e-15 74.7
Msa1447900 MtrunA17_Chr4g0050781 45.833 72 35 1 20 91 3 70 7.86e-15 71.2
Msa1447900 MtrunA17_Chr7g0219031 49.153 59 30 0 25 83 41 99 1.05e-14 68.6
Msa1447900 MtrunA17_Chr5g0447761 46.667 60 32 0 25 84 7 66 1.08e-14 73.2
Msa1447900 MtrunA17_Chr4g0035031 43.421 76 42 1 25 99 9 84 1.10e-14 70.5
Msa1447900 MtrunA17_Chr1g0186351 37.500 80 49 1 20 99 2 80 1.13e-14 73.2
Msa1447900 MtrunA17_Chr3g0119311 36.957 92 54 2 24 114 36 124 1.17e-14 68.9
Msa1447900 MtrunA17_Chr3g0135111 42.500 80 45 1 20 99 6 84 2.66e-14 71.6
Msa1447900 MtrunA17_Chr4g0035001 47.458 59 31 0 25 83 7 65 3.38e-14 68.6
Msa1447900 MtrunA17_Chr4g0031091 42.500 80 45 1 24 102 33 112 1.94e-13 65.1
Msa1447900 MtrunA17_Chr1g0198431 37.755 98 60 1 3 99 171 268 3.63e-13 67.8
Msa1447900 MtrunA17_Chr1g0210241 36.905 84 49 1 24 107 7 86 3.15e-12 65.1
Msa1447900 MtrunA17_Chr4g0042161 45.000 60 33 0 24 83 51 110 3.40e-12 62.4
Msa1447900 MtrunA17_Chr3g0120721 40.625 64 38 0 25 88 19 82 8.55e-12 64.3
Msa1447900 MtrunA17_Chr3g0088511 44.828 58 32 0 24 81 32 89 8.71e-12 61.2
Msa1447900 MtrunA17_Chr6g0449481 42.188 64 37 0 25 88 46 109 3.38e-11 62.8
Msa1447900 MtrunA17_Chr7g0276201 47.541 61 32 0 24 84 104 164 5.25e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1447900 AT3G54770.1 47.600 250 108 8 6 244 2 239 1.37e-55 179
Msa1447900 AT3G54770.3 47.600 250 108 8 6 244 2 239 2.29e-55 179
Msa1447900 AT1G76460.1 75.824 91 22 0 16 106 16 106 1.18e-45 154
Msa1447900 AT1G20880.2 75.824 91 22 0 16 106 16 106 1.26e-45 154
Msa1447900 AT1G20880.3 75.824 91 22 0 16 106 16 106 1.26e-45 154
Msa1447900 AT1G20880.1 75.824 91 22 0 16 106 16 106 1.26e-45 154
Msa1447900 AT1G22910.2 67.391 92 30 0 18 109 7 98 1.62e-41 142
Msa1447900 AT1G22910.1 67.391 92 30 0 18 109 7 98 2.32e-41 142
Msa1447900 AT3G54770.2 46.445 211 92 8 40 244 1 196 2.43e-41 141
Msa1447900 AT2G46780.1 63.810 105 31 2 10 114 13 110 8.54e-41 143
Msa1447900 AT2G46780.2 63.810 105 31 2 10 114 13 110 8.54e-41 143
Msa1447900 AT1G22910.3 67.391 92 30 0 18 109 7 98 2.07e-40 142
Msa1447900 AT1G33470.1 67.742 93 29 1 20 111 3 95 1.06e-39 138
Msa1447900 AT1G33470.3 67.742 93 29 1 20 111 3 95 1.12e-39 138
Msa1447900 AT1G33470.2 67.742 93 29 1 20 111 3 95 1.19e-39 138
Msa1447900 AT1G22330.1 65.934 91 31 0 16 106 9 99 1.47e-39 139
Msa1447900 AT1G78260.1 66.279 86 29 0 16 101 9 94 1.32e-37 134
Msa1447900 AT1G78260.2 66.279 86 29 0 16 101 9 94 1.67e-37 133
Msa1447900 AT5G11412.1 54.118 85 39 0 20 104 48 132 1.25e-29 110
Msa1447900 AT5G53680.1 50.588 85 42 0 20 104 9 93 2.57e-26 101
Msa1447900 AT3G06970.1 46.154 91 49 0 20 110 8 98 7.46e-24 98.2
Msa1447900 AT5G53720.1 49.383 81 41 0 24 104 9 89 1.60e-23 91.7
Msa1447900 AT2G22090.2 49.398 83 42 0 20 102 100 182 1.81e-19 86.7
Msa1447900 AT2G22090.1 49.398 83 42 0 20 102 100 182 1.89e-19 86.7
Msa1447900 AT3G15010.1 37.500 96 60 0 9 104 152 247 1.82e-18 84.3
Msa1447900 AT3G15010.1 46.988 83 44 0 20 102 71 153 1.09e-17 82.0
Msa1447900 AT3G15010.2 37.500 96 60 0 9 104 152 247 1.82e-18 84.3
Msa1447900 AT3G15010.2 46.988 83 44 0 20 102 71 153 1.09e-17 82.0
Msa1447900 AT3G23830.2 41.379 87 50 1 24 109 35 121 3.51e-18 79.0
Msa1447900 AT3G23830.1 41.379 87 50 1 24 109 35 121 3.51e-18 79.0
Msa1447900 AT4G39260.3 40.000 85 47 1 25 109 7 87 5.66e-18 77.0
Msa1447900 AT4G26650.2 44.444 72 39 1 21 92 9 79 2.83e-17 81.3
Msa1447900 AT4G26650.1 44.444 72 39 1 21 92 12 82 2.94e-17 80.9
Msa1447900 AT2G41060.1 36.792 106 61 2 20 121 124 227 6.79e-17 80.1
Msa1447900 AT2G41060.3 36.792 106 61 2 20 121 124 227 6.79e-17 80.1
Msa1447900 AT2G41060.2 36.792 106 61 2 20 121 124 227 6.79e-17 80.1
Msa1447900 AT4G39260.2 41.558 77 41 1 25 101 7 79 7.41e-17 75.1
Msa1447900 AT4G39260.4 50.000 60 30 0 25 84 7 66 7.76e-17 74.3
Msa1447900 AT4G14300.1 44.928 69 38 0 20 88 2 70 1.38e-16 79.0
Msa1447900 AT4G14300.2 44.928 69 38 0 20 88 2 70 1.38e-16 79.0
Msa1447900 AT4G39260.1 41.558 77 41 1 25 101 7 79 2.02e-16 75.1
Msa1447900 AT4G13850.4 45.333 75 40 1 24 97 35 109 2.47e-16 73.6
Msa1447900 AT5G55550.5 41.096 73 42 1 20 92 2 73 2.75e-16 78.2
Msa1447900 AT5G55550.4 41.096 73 42 1 20 92 2 73 2.75e-16 78.2
Msa1447900 AT5G55550.9 41.096 73 42 1 20 92 2 73 2.75e-16 78.2
Msa1447900 AT5G55550.7 41.096 73 42 1 20 92 2 73 2.75e-16 78.2
Msa1447900 AT5G55550.1 41.096 73 42 1 20 92 2 73 2.75e-16 78.2
Msa1447900 AT5G55550.8 41.096 73 42 1 20 92 2 73 2.84e-16 78.2
Msa1447900 AT5G55550.2 41.096 73 42 1 20 92 2 73 3.17e-16 78.2
Msa1447900 AT5G55550.3 41.096 73 42 1 20 92 2 73 3.17e-16 78.2
Msa1447900 AT5G55550.6 41.096 73 42 1 20 92 2 73 3.17e-16 78.2
Msa1447900 AT5G55550.11 41.096 73 42 1 20 92 2 73 3.17e-16 78.2
Msa1447900 AT5G55550.10 41.096 73 42 1 20 92 2 73 3.30e-16 78.2
Msa1447900 AT2G21660.2 54.237 59 27 0 25 83 9 67 3.34e-16 74.3
Msa1447900 AT4G13850.3 45.333 75 40 1 24 97 35 109 3.58e-16 73.6
Msa1447900 AT4G13850.2 45.333 75 40 1 24 97 35 109 4.40e-16 73.6
Msa1447900 AT2G19380.1 30.337 178 105 4 20 180 404 579 4.46e-16 77.8
Msa1447900 AT2G21660.1 54.237 59 27 0 25 83 9 67 4.81e-16 74.3
Msa1447900 AT5G47620.1 45.312 64 35 0 25 88 7 70 4.88e-16 77.4
Msa1447900 AT5G47620.2 45.312 64 35 0 25 88 7 70 4.88e-16 77.4
Msa1447900 AT5G47620.5 45.312 64 35 0 25 88 7 70 4.88e-16 77.4
Msa1447900 AT4G13850.1 45.333 75 40 1 24 97 35 109 4.93e-16 73.6
Msa1447900 AT5G47620.4 45.312 64 35 0 25 88 7 70 5.46e-16 77.4
Msa1447900 AT3G56860.9 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.11 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.8 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.5 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.7 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.2 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.6 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.10 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.3 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.1 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT3G56860.4 42.308 78 45 0 25 102 141 218 5.46e-16 77.4
Msa1447900 AT1G17640.2 29.771 131 80 2 25 147 67 193 1.27e-15 75.9
Msa1447900 AT1G17640.1 29.771 131 80 2 25 147 67 193 1.27e-15 75.9
Msa1447900 AT1G17640.3 29.771 131 80 2 25 147 67 193 1.27e-15 75.9
Msa1447900 AT1G74230.1 44.156 77 43 0 24 100 34 110 2.02e-15 74.7
Msa1447900 AT2G22100.1 35.135 111 69 1 20 127 159 269 2.23e-15 75.1
Msa1447900 AT3G26420.1 47.761 67 31 1 25 91 8 70 3.38e-15 73.2
Msa1447900 AT5G61030.2 44.928 69 34 1 24 92 40 104 3.42e-15 74.3
Msa1447900 AT5G61030.1 44.928 69 34 1 24 92 40 104 3.42e-15 74.3
Msa1447900 AT3G07810.1 34.615 104 56 3 25 128 7 98 2.15e-14 72.8
Msa1447900 AT5G06210.1 45.833 72 35 1 24 95 34 101 2.39e-14 68.9
Msa1447900 AT3G07810.2 34.615 104 56 3 25 128 7 98 2.55e-14 72.4
Msa1447900 AT4G13860.1 39.024 82 45 2 24 102 3 82 1.64e-13 65.1
Msa1447900 AT3G13224.2 39.362 94 52 3 4 96 1 90 3.90e-13 68.6
Msa1447900 AT5G59865.1 50.980 51 25 0 22 72 24 74 1.37e-12 63.2
Msa1447900 AT2G33410.1 33.803 71 47 0 20 90 2 72 1.43e-12 67.0
Msa1447900 AT3G13224.1 39.362 94 52 3 4 96 1 90 1.54e-12 65.5
Msa1447900 AT3G08000.1 33.654 104 61 3 25 125 42 140 2.97e-12 63.2
Msa1447900 AT1G58470.2 41.429 70 41 0 20 89 2 71 3.21e-12 65.9
Msa1447900 AT1G58470.1 41.429 70 41 0 20 89 2 71 3.21e-12 65.9
Msa1447900 AT5G40490.1 44.615 65 36 0 25 89 43 107 5.34e-12 65.5
Msa1447900 AT3G13224.3 40.845 71 41 1 25 95 11 80 8.96e-12 64.7
Msa1447900 AT3G20930.1 40.909 88 50 2 25 110 283 370 1.51e-11 63.9
Msa1447900 AT1G18630.1 41.791 67 37 1 19 83 29 95 1.57e-11 61.2
Msa1447900 AT4G36960.1 40.678 59 35 0 25 83 4 62 4.89e-11 62.4
Msa1447900 AT4G36960.2 40.678 59 35 0 25 83 4 62 4.89e-11 62.4
Msa1447900 AT4G20030.1 36.458 96 60 1 24 118 40 135 6.43e-11 59.7

Find 112 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCAGTAACAAAAGTATT+TGG 0.196601 tig0031948:-3963 None:intergenic
GCGTGATGATGATGGAATAT+TGG 0.316199 tig0031948:-4497 None:intergenic
GGAGGAGGAGGAGAAGTTAT+GGG 0.316894 tig0031948:-4404 None:intergenic
TGGAGGAGGAGGAGAAGTTA+TGG 0.320433 tig0031948:-4405 None:intergenic
CGTGATGATGATGGAATATT+GGG 0.323951 tig0031948:-4496 None:intergenic
ATGGATCACCATCACAGTTT+GGG 0.341928 tig0031948:+4111 Msa1447900:CDS
AATGGATCACCATCACAGTT+TGG 0.360186 tig0031948:+4110 Msa1447900:CDS
TGTTCTCTTAATGTGTCTTT+CGG 0.360765 tig0031948:-4182 None:intergenic
TGGTTGCTGCGGTGGATCTT+TGG 0.373820 tig0031948:-4425 None:intergenic
ATAATTTCTGATAAGGTTAC+CGG 0.382398 tig0031948:+4239 Msa1447900:CDS
TGTTTGTTGGAGGTCTTGCC+TGG 0.388391 tig0031948:+4153 Msa1447900:CDS
AGCTGTCATAATTTCTGATA+AGG 0.401698 tig0031948:+4232 Msa1447900:CDS
TGTTACAAATCCATAGCCTT+TGG 0.401760 tig0031948:-4267 None:intergenic
AAGCATTCACCCGTGCCAAC+TGG 0.407073 tig0031948:-5174 None:intergenic
CATCACGCCCTTCCTTTCTA+CGG 0.413597 tig0031948:+4512 Msa1447900:CDS
CCTGCTGCTGGTTGCTGCGG+TGG 0.413826 tig0031948:-4433 None:intergenic
ACTCAGTAACAAAAGTATTT+GGG 0.422425 tig0031948:-3962 None:intergenic
CTCTTGGTGCTCGCCGTCCA+AGG 0.426692 tig0031948:+4375 Msa1447900:CDS
AATAATGATAATGATGAGTT+GGG 0.437625 tig0031948:-5007 None:intergenic
TTACAACTGCAATTTCAATC+AGG 0.444679 tig0031948:+4735 Msa1447900:CDS
CAGAAATTATGACAGCTTCA+AGG 0.453018 tig0031948:-4226 None:intergenic
CCGCCGAGGCAGGCTATTGT+GGG 0.455950 tig0031948:+4891 Msa1447900:CDS
ACGCTGACAAAAGTGTTTGT+TGG 0.457047 tig0031948:+4140 Msa1447900:CDS
AAGGAAGGGCGTGATGATGA+TGG 0.457080 tig0031948:-4505 None:intergenic
TCCATAGCCTTTGGATCTGC+CGG 0.474152 tig0031948:-4258 None:intergenic
GAATAATGATAATGATGAGT+TGG 0.476228 tig0031948:-5008 None:intergenic
GATCTGTAGACAGGAGGAGC+AGG 0.479037 tig0031948:-4939 None:intergenic
GAATATTGGGGAAGGAGGTG+TGG 0.480515 tig0031948:-4483 None:intergenic
GCACGGGTGAATGCTTCAAA+AGG 0.482053 tig0031948:+5181 Msa1447900:CDS
ACTCCTAGTCCTACTACAAC+AGG 0.483068 tig0031948:+5041 Msa1447900:CDS
GGGTGGAAGATTTGAGGTGG+AGG 0.485695 tig0031948:-4987 None:intergenic
AGATGCAGAGGCTGCAAAGA+AGG 0.488455 tig0031948:+4295 Msa1447900:CDS
TGTGTCTTTCGGAGTTTCCC+AGG 0.489644 tig0031948:-4171 None:intergenic
CCACAATAGCCTGCCTCGGC+GGG 0.493685 tig0031948:-4890 None:intergenic
GCAGGGTGGTGGTACATGAT+TGG 0.499499 tig0031948:-4921 None:intergenic
ATCACGCCCTTCCTTTCTAC+GGG 0.499631 tig0031948:+4513 Msa1447900:CDS
CCACCGCAGCAACCAGCAGC+AGG 0.500038 tig0031948:+4433 Msa1447900:CDS
ATCAACCGAGGCTATGGCCA+TGG 0.501706 tig0031948:+4959 Msa1447900:CDS
ATATATTAACCTGTTGTAGT+AGG 0.505308 tig0031948:-5050 None:intergenic
CTACAGATCAACCGAGGCTA+TGG 0.506662 tig0031948:+4953 Msa1447900:CDS
TTGCTGCGGTGGATCTTTGG+AGG 0.509090 tig0031948:-4422 None:intergenic
CTGAGCTATGGAATGGGTGG+GGG 0.510253 tig0031948:+4858 Msa1447900:CDS
CGGTGGATCTTTGGAGGAGG+AGG 0.513479 tig0031948:-4416 None:intergenic
TAAAATATATACCCGTAGAA+AGG 0.515467 tig0031948:-4524 None:intergenic
CATATGATCTGTTGCAATGT+AGG 0.515946 tig0031948:-4710 None:intergenic
GCCTCGGTTGATCTGTAGAC+AGG 0.521868 tig0031948:-4948 None:intergenic
ATATATACCCGTAGAAAGGA+AGG 0.523229 tig0031948:-4520 None:intergenic
AACTGCAATATCGCTTCTCT+TGG 0.523354 tig0031948:+4359 Msa1447900:CDS
TAGTCAGTCTATAAGAAAGA+AGG 0.524612 tig0031948:-4015 None:intergenic
GCAGGTTCAATGGTATTATC+CGG 0.525480 tig0031948:+4451 Msa1447900:CDS
AACTGAGCTATGGAATGGGT+GGG 0.529212 tig0031948:+4856 Msa1447900:CDS
TAACCTGTTGTAGTAGGACT+AGG 0.534126 tig0031948:-5044 None:intergenic
TAGGACTAGGAGTGATGGAT+GGG 0.535767 tig0031948:-5031 None:intergenic
GGAGGTGTGGGTGTTCCCAC+CGG 0.538024 tig0031948:-4470 None:intergenic
ACCGGCAGATCCAAAGGCTA+TGG 0.539327 tig0031948:+4257 Msa1447900:CDS
AGACAGGAGGAGCAGGGTGG+TGG 0.540978 tig0031948:-4932 None:intergenic
CTGACAAAAGTGTTTGTTGG+AGG 0.542823 tig0031948:+4143 Msa1447900:CDS
GCAACCAGCAGCAGGTTCAA+TGG 0.542889 tig0031948:+4441 Msa1447900:CDS
GACACCCATGGCCATAGCCT+CGG 0.543694 tig0031948:-4964 None:intergenic
ACTGAGCTATGGAATGGGTG+GGG 0.544490 tig0031948:+4857 Msa1447900:CDS
AATATTGGGGAAGGAGGTGT+GGG 0.546556 tig0031948:-4482 None:intergenic
CTGCGGTGGATCTTTGGAGG+AGG 0.548182 tig0031948:-4419 None:intergenic
GATATTGATCATGACGATGA+TGG 0.548742 tig0031948:+4067 Msa1447900:exon
GAACCAACTGTGATCATCAA+TGG 0.551058 tig0031948:+4326 Msa1447900:CDS
ATTGATAGGTCCAGTTGGCA+CGG 0.551120 tig0031948:+5164 Msa1447900:intron
AATACCATTGAACCTGCTGC+TGG 0.553977 tig0031948:-4445 None:intergenic
AAGCGATATTGCAGTTGGCT+CGG 0.556799 tig0031948:-4352 None:intergenic
TTGATAGGTCCAGTTGGCAC+GGG 0.560846 tig0031948:+5165 Msa1447900:intron
GAAGTTATGGGAGAAGACCT+TGG 0.565897 tig0031948:-4392 None:intergenic
CGAATGCAGAAACTGAGCTA+TGG 0.568591 tig0031948:+4846 Msa1447900:intron
CCCACAATAGCCTGCCTCGG+CGG 0.568725 tig0031948:-4891 None:intergenic
AAACTGAGCTATGGAATGGG+TGG 0.571141 tig0031948:+4855 Msa1447900:CDS
GTAGGACTAGGAGTGATGGA+TGG 0.572210 tig0031948:-5032 None:intergenic
GAACCTGCTGCTGGTTGCTG+CGG 0.576807 tig0031948:-4436 None:intergenic
CGACCATTGATGATCACAGT+TGG 0.578434 tig0031948:-4329 None:intergenic
AAGGTTACCGGCAGATCCAA+AGG 0.581260 tig0031948:+4251 Msa1447900:CDS
TCAACCGAGGCTATGGCCAT+GGG 0.585523 tig0031948:+4960 Msa1447900:CDS
GGTGGAAGATTTGAGGTGGA+GGG 0.586269 tig0031948:-4986 None:intergenic
TGAGTTGGGTGGAAGATTTG+AGG 0.586506 tig0031948:-4993 None:intergenic
TGATGGAATATTGGGGAAGG+AGG 0.587373 tig0031948:-4488 None:intergenic
TGAATGCTTCAAAAGGGTAG+TGG 0.590972 tig0031948:+5188 Msa1447900:CDS
GGCTGCAAAGAAGGCTTGCG+AGG 0.592252 tig0031948:+4304 Msa1447900:CDS
TGAATATTGATAGGTCCAGT+TGG 0.592422 tig0031948:+5159 Msa1447900:intron
GCAGAAACTGAGCTATGGAA+TGG 0.594468 tig0031948:+4851 Msa1447900:intron
AAGAGAAGCGATATTGCAGT+TGG 0.596190 tig0031948:-4357 None:intergenic
TGTAGTAGGACTAGGAGTGA+TGG 0.600167 tig0031948:-5036 None:intergenic
GTTGGGTGGAAGATTTGAGG+TGG 0.600463 tig0031948:-4990 None:intergenic
GCACCCACAATAGCCTGCCT+CGG 0.602284 tig0031948:-4894 None:intergenic
ATGATCTGTTGCAATGTAGG+TGG 0.608013 tig0031948:-4707 None:intergenic
GGTTCAATGGTATTATCCGG+TGG 0.611241 tig0031948:+4454 Msa1447900:CDS
CAGAAACTGAGCTATGGAAT+GGG 0.612059 tig0031948:+4852 Msa1447900:intron
TGTAACATTCAAAGATGCAG+AGG 0.612207 tig0031948:+4283 Msa1447900:CDS
CACGGGTGAATGCTTCAAAA+GGG 0.613875 tig0031948:+5182 Msa1447900:CDS
ATATTGATCATGACGATGAT+GGG 0.616366 tig0031948:+4068 Msa1447900:exon
TTGAGGTGGAGGGACACCCA+TGG 0.627473 tig0031948:-4976 None:intergenic
TATATACCCGTAGAAAGGAA+GGG 0.631562 tig0031948:-4519 None:intergenic
ATCTGTAGACAGGAGGAGCA+GGG 0.632459 tig0031948:-4938 None:intergenic
GTTTGTTGGAGGTCTTGCCT+GGG 0.633552 tig0031948:+4154 Msa1447900:CDS
GTTCAATGGTATTATCCGGT+GGG 0.634469 tig0031948:+4455 Msa1447900:CDS
TGATCTGTTGCAATGTAGGT+GGG 0.635848 tig0031948:-4706 None:intergenic
AATGATAATGATGAGTTGGG+TGG 0.637114 tig0031948:-5004 None:intergenic
TGATGATGGAATATTGGGGA+AGG 0.639851 tig0031948:-4491 None:intergenic
TTATGGGAGAAGACCTTGGA+CGG 0.649901 tig0031948:-4388 None:intergenic
GTTGTGTCCCCAAACTGTGA+TGG 0.652121 tig0031948:-4119 None:intergenic
AGCAGCAACAGCAACAACAA+TGG 0.659160 tig0031948:+4092 Msa1447900:CDS
GGGGCTACGTGTACCCGCCG+AGG 0.659428 tig0031948:+4877 Msa1447900:CDS
AGGACTAGGAGTGATGGATG+GGG 0.665517 tig0031948:-5030 None:intergenic
TGGATCACCATCACAGTTTG+GGG 0.666646 tig0031948:+4112 Msa1447900:CDS
TCGGTTGATCTGTAGACAGG+AGG 0.670555 tig0031948:-4945 None:intergenic
TCCTGTCTACAGATCAACCG+AGG 0.712533 tig0031948:+4947 Msa1447900:CDS
GTGATGATGATGGAATATTG+GGG 0.716207 tig0031948:-4495 None:intergenic
TGTAGACAGGAGGAGCAGGG+TGG 0.747299 tig0031948:-4935 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTCTTGTTATTGAATT+AGG - tig0031948:4797-4816 None:intergenic 15.0%
!!! TTTTAATTTGAAAAAATGGT+TGG + tig0031948:4822-4841 Msa1447900:intron 15.0%
!!! ATATTTCTTGTTATTGAATT+AGG - tig0031948:4797-4816 None:intergenic 15.0%
!!! TTTTAATTTGAAAAAATGGT+TGG + tig0031948:4822-4841 Msa1447900:intron 15.0%
!!! CATTTAAATCACATTTGTTA+AGG - tig0031948:4656-4675 None:intergenic 20.0%
!!! AATAATGATAATGATGAGTT+GGG - tig0031948:5010-5029 None:intergenic 20.0%
!!! CATTTAAATCACATTTGTTA+AGG - tig0031948:4656-4675 None:intergenic 20.0%
!!! AATAATGATAATGATGAGTT+GGG - tig0031948:5010-5029 None:intergenic 20.0%
!! AGAGAACACTTTGAAAAATA+CGG + tig0031948:4197-4216 Msa1447900:CDS 25.0%
! ATAATTTCTGATAAGGTTAC+CGG + tig0031948:4239-4258 Msa1447900:CDS 25.0%
! TAAAATATATACCCGTAGAA+AGG - tig0031948:4527-4546 None:intergenic 25.0%
!! TAAATCACATTTGTTAAGGT+CGG - tig0031948:4652-4671 None:intergenic 25.0%
!! GAATAATGATAATGATGAGT+TGG - tig0031948:5011-5030 None:intergenic 25.0%
! ATATATTAACCTGTTGTAGT+AGG - tig0031948:5053-5072 None:intergenic 25.0%
!! AGAGAACACTTTGAAAAATA+CGG + tig0031948:4197-4216 Msa1447900:CDS 25.0%
! ATAATTTCTGATAAGGTTAC+CGG + tig0031948:4239-4258 Msa1447900:CDS 25.0%
! TAAAATATATACCCGTAGAA+AGG - tig0031948:4527-4546 None:intergenic 25.0%
!! TAAATCACATTTGTTAAGGT+CGG - tig0031948:4652-4671 None:intergenic 25.0%
!! GAATAATGATAATGATGAGT+TGG - tig0031948:5011-5030 None:intergenic 25.0%
! ATATATTAACCTGTTGTAGT+AGG - tig0031948:5053-5072 None:intergenic 25.0%
!! CACTCAGTAACAAAAGTATT+TGG - tig0031948:3966-3985 None:intergenic 30.0%
TAGTCAGTCTATAAGAAAGA+AGG - tig0031948:4018-4037 None:intergenic 30.0%
ATATTGATCATGACGATGAT+GGG + tig0031948:4068-4087 Msa1447900:exon 30.0%
TGTTCTCTTAATGTGTCTTT+CGG - tig0031948:4185-4204 None:intergenic 30.0%
AGCTGTCATAATTTCTGATA+AGG + tig0031948:4232-4251 Msa1447900:CDS 30.0%
! AAATCACATTTGTTAAGGTC+GGG - tig0031948:4651-4670 None:intergenic 30.0%
TTACAACTGCAATTTCAATC+AGG + tig0031948:4735-4754 Msa1447900:CDS 30.0%
GGCATGCAATGTAATAATAT+TGG - tig0031948:4776-4795 None:intergenic 30.0%
!! CACTCAGTAACAAAAGTATT+TGG - tig0031948:3966-3985 None:intergenic 30.0%
TAGTCAGTCTATAAGAAAGA+AGG - tig0031948:4018-4037 None:intergenic 30.0%
ATATTGATCATGACGATGAT+GGG + tig0031948:4068-4087 Msa1447900:exon 30.0%
TGTTCTCTTAATGTGTCTTT+CGG - tig0031948:4185-4204 None:intergenic 30.0%
AGCTGTCATAATTTCTGATA+AGG + tig0031948:4232-4251 Msa1447900:CDS 30.0%
! AAATCACATTTGTTAAGGTC+GGG - tig0031948:4651-4670 None:intergenic 30.0%
TTACAACTGCAATTTCAATC+AGG + tig0031948:4735-4754 Msa1447900:CDS 30.0%
GGCATGCAATGTAATAATAT+TGG - tig0031948:4776-4795 None:intergenic 30.0%
GATATTGATCATGACGATGA+TGG + tig0031948:4067-4086 Msa1447900:exon 35.0%
CAGAAATTATGACAGCTTCA+AGG - tig0031948:4229-4248 None:intergenic 35.0%
TGTTACAAATCCATAGCCTT+TGG - tig0031948:4270-4289 None:intergenic 35.0%
TGTAACATTCAAAGATGCAG+AGG + tig0031948:4283-4302 Msa1447900:CDS 35.0%
!! GTGATGATGATGGAATATTG+GGG - tig0031948:4498-4517 None:intergenic 35.0%
!! CGTGATGATGATGGAATATT+GGG - tig0031948:4499-4518 None:intergenic 35.0%
TATATACCCGTAGAAAGGAA+GGG - tig0031948:4522-4541 None:intergenic 35.0%
ATATATACCCGTAGAAAGGA+AGG - tig0031948:4523-4542 None:intergenic 35.0%
!!! CTTGTTTGTTTTGGGTGAAA+GGG + tig0031948:4619-4638 Msa1447900:intron 35.0%
ATCTCCTCTTTCTTTCATTC+AGG + tig0031948:4677-4696 Msa1447900:intron 35.0%
TGTACCTGAATGAAAGAAAG+AGG - tig0031948:4684-4703 None:intergenic 35.0%
CATATGATCTGTTGCAATGT+AGG - tig0031948:4713-4732 None:intergenic 35.0%
ACTGCAATTTCAATCAGGTA+TGG + tig0031948:4740-4759 Msa1447900:intron 35.0%
! AATGATAATGATGAGTTGGG+TGG - tig0031948:5007-5026 None:intergenic 35.0%
AAAGTGACAGTTTGTGTTTG+TGG + tig0031948:5132-5151 Msa1447900:intron 35.0%
AAGTGACAGTTTGTGTTTGT+GGG + tig0031948:5133-5152 Msa1447900:intron 35.0%
!! TGTGGGTGTTGAATATTGAT+AGG + tig0031948:5150-5169 Msa1447900:intron 35.0%
TGAATATTGATAGGTCCAGT+TGG + tig0031948:5159-5178 Msa1447900:intron 35.0%
GATATTGATCATGACGATGA+TGG + tig0031948:4067-4086 Msa1447900:exon 35.0%
CAGAAATTATGACAGCTTCA+AGG - tig0031948:4229-4248 None:intergenic 35.0%
TGTTACAAATCCATAGCCTT+TGG - tig0031948:4270-4289 None:intergenic 35.0%
TGTAACATTCAAAGATGCAG+AGG + tig0031948:4283-4302 Msa1447900:CDS 35.0%
!! GTGATGATGATGGAATATTG+GGG - tig0031948:4498-4517 None:intergenic 35.0%
!! CGTGATGATGATGGAATATT+GGG - tig0031948:4499-4518 None:intergenic 35.0%
TATATACCCGTAGAAAGGAA+GGG - tig0031948:4522-4541 None:intergenic 35.0%
ATATATACCCGTAGAAAGGA+AGG - tig0031948:4523-4542 None:intergenic 35.0%
!!! CTTGTTTGTTTTGGGTGAAA+GGG + tig0031948:4619-4638 Msa1447900:intron 35.0%
ATCTCCTCTTTCTTTCATTC+AGG + tig0031948:4677-4696 Msa1447900:intron 35.0%
TGTACCTGAATGAAAGAAAG+AGG - tig0031948:4684-4703 None:intergenic 35.0%
CATATGATCTGTTGCAATGT+AGG - tig0031948:4713-4732 None:intergenic 35.0%
ACTGCAATTTCAATCAGGTA+TGG + tig0031948:4740-4759 Msa1447900:intron 35.0%
! AATGATAATGATGAGTTGGG+TGG - tig0031948:5007-5026 None:intergenic 35.0%
AAAGTGACAGTTTGTGTTTG+TGG + tig0031948:5132-5151 Msa1447900:intron 35.0%
AAGTGACAGTTTGTGTTTGT+GGG + tig0031948:5133-5152 Msa1447900:intron 35.0%
!! TGTGGGTGTTGAATATTGAT+AGG + tig0031948:5150-5169 Msa1447900:intron 35.0%
TGAATATTGATAGGTCCAGT+TGG + tig0031948:5159-5178 Msa1447900:intron 35.0%
AATGGATCACCATCACAGTT+TGG + tig0031948:4110-4129 Msa1447900:CDS 40.0%
ATGGATCACCATCACAGTTT+GGG + tig0031948:4111-4130 Msa1447900:CDS 40.0%
!! ACGCTGACAAAAGTGTTTGT+TGG + tig0031948:4140-4159 Msa1447900:CDS 40.0%
!! CTGACAAAAGTGTTTGTTGG+AGG + tig0031948:4143-4162 Msa1447900:CDS 40.0%
GAACCAACTGTGATCATCAA+TGG + tig0031948:4326-4345 Msa1447900:CDS 40.0%
AAGAGAAGCGATATTGCAGT+TGG - tig0031948:4360-4379 None:intergenic 40.0%
AACTGCAATATCGCTTCTCT+TGG + tig0031948:4359-4378 Msa1447900:CDS 40.0%
GCAGGTTCAATGGTATTATC+CGG + tig0031948:4451-4470 Msa1447900:CDS 40.0%
GTTCAATGGTATTATCCGGT+GGG + tig0031948:4455-4474 Msa1447900:CDS 40.0%
!! TGATGATGGAATATTGGGGA+AGG - tig0031948:4494-4513 None:intergenic 40.0%
!! GCGTGATGATGATGGAATAT+TGG - tig0031948:4500-4519 None:intergenic 40.0%
AGAGAGAGAGAGAGAGATAT+GGG + tig0031948:4591-4610 Msa1447900:intron 40.0%
!! TGGGACATCCTTGTTTGTTT+TGG + tig0031948:4610-4629 Msa1447900:intron 40.0%
!! GGGACATCCTTGTTTGTTTT+GGG + tig0031948:4611-4630 Msa1447900:intron 40.0%
CCTTTCACCCAAAACAAACA+AGG - tig0031948:4621-4640 None:intergenic 40.0%
!!! CCTTGTTTGTTTTGGGTGAA+AGG + tig0031948:4618-4637 Msa1447900:intron 40.0%
TGATCTGTTGCAATGTAGGT+GGG - tig0031948:4709-4728 None:intergenic 40.0%
ATGATCTGTTGCAATGTAGG+TGG - tig0031948:4710-4729 None:intergenic 40.0%
CAGAAACTGAGCTATGGAAT+GGG + tig0031948:4852-4871 Msa1447900:intron 40.0%
TAACCTGTTGTAGTAGGACT+AGG - tig0031948:5047-5066 None:intergenic 40.0%
TGAATGCTTCAAAAGGGTAG+TGG + tig0031948:5188-5207 Msa1447900:CDS 40.0%
AATGGATCACCATCACAGTT+TGG + tig0031948:4110-4129 Msa1447900:CDS 40.0%
ATGGATCACCATCACAGTTT+GGG + tig0031948:4111-4130 Msa1447900:CDS 40.0%
!! ACGCTGACAAAAGTGTTTGT+TGG + tig0031948:4140-4159 Msa1447900:CDS 40.0%
!! CTGACAAAAGTGTTTGTTGG+AGG + tig0031948:4143-4162 Msa1447900:CDS 40.0%
GAACCAACTGTGATCATCAA+TGG + tig0031948:4326-4345 Msa1447900:CDS 40.0%
AAGAGAAGCGATATTGCAGT+TGG - tig0031948:4360-4379 None:intergenic 40.0%
AACTGCAATATCGCTTCTCT+TGG + tig0031948:4359-4378 Msa1447900:CDS 40.0%
GCAGGTTCAATGGTATTATC+CGG + tig0031948:4451-4470 Msa1447900:CDS 40.0%
GTTCAATGGTATTATCCGGT+GGG + tig0031948:4455-4474 Msa1447900:CDS 40.0%
!! TGATGATGGAATATTGGGGA+AGG - tig0031948:4494-4513 None:intergenic 40.0%
!! GCGTGATGATGATGGAATAT+TGG - tig0031948:4500-4519 None:intergenic 40.0%
AGAGAGAGAGAGAGAGATAT+GGG + tig0031948:4591-4610 Msa1447900:intron 40.0%
!! TGGGACATCCTTGTTTGTTT+TGG + tig0031948:4610-4629 Msa1447900:intron 40.0%
!! GGGACATCCTTGTTTGTTTT+GGG + tig0031948:4611-4630 Msa1447900:intron 40.0%
CCTTTCACCCAAAACAAACA+AGG - tig0031948:4621-4640 None:intergenic 40.0%
!!! CCTTGTTTGTTTTGGGTGAA+AGG + tig0031948:4618-4637 Msa1447900:intron 40.0%
TGATCTGTTGCAATGTAGGT+GGG - tig0031948:4709-4728 None:intergenic 40.0%
ATGATCTGTTGCAATGTAGG+TGG - tig0031948:4710-4729 None:intergenic 40.0%
CAGAAACTGAGCTATGGAAT+GGG + tig0031948:4852-4871 Msa1447900:intron 40.0%
TAACCTGTTGTAGTAGGACT+AGG - tig0031948:5047-5066 None:intergenic 40.0%
TGAATGCTTCAAAAGGGTAG+TGG + tig0031948:5188-5207 Msa1447900:CDS 40.0%
AGCAGCAACAGCAACAACAA+TGG + tig0031948:4092-4111 Msa1447900:CDS 45.0%
TGGATCACCATCACAGTTTG+GGG + tig0031948:4112-4131 Msa1447900:CDS 45.0%
! CGACCATTGATGATCACAGT+TGG - tig0031948:4332-4351 None:intergenic 45.0%
AAGCGATATTGCAGTTGGCT+CGG - tig0031948:4355-4374 None:intergenic 45.0%
TTATGGGAGAAGACCTTGGA+CGG - tig0031948:4391-4410 None:intergenic 45.0%
GAAGTTATGGGAGAAGACCT+TGG - tig0031948:4395-4414 None:intergenic 45.0%
AATACCATTGAACCTGCTGC+TGG - tig0031948:4448-4467 None:intergenic 45.0%
GGTTCAATGGTATTATCCGG+TGG + tig0031948:4454-4473 Msa1447900:CDS 45.0%
! AATATTGGGGAAGGAGGTGT+GGG - tig0031948:4485-4504 None:intergenic 45.0%
TGATGGAATATTGGGGAAGG+AGG - tig0031948:4491-4510 None:intergenic 45.0%
CATATCTCTCTCTCTCTCTC+TGG - tig0031948:4592-4611 None:intergenic 45.0%
CAGAGAGAGAGAGAGAGATA+TGG + tig0031948:4590-4609 Msa1447900:intron 45.0%
CGAATGCAGAAACTGAGCTA+TGG + tig0031948:4846-4865 Msa1447900:intron 45.0%
GCAGAAACTGAGCTATGGAA+TGG + tig0031948:4851-4870 Msa1447900:intron 45.0%
AAACTGAGCTATGGAATGGG+TGG + tig0031948:4855-4874 Msa1447900:CDS 45.0%
AACTGAGCTATGGAATGGGT+GGG + tig0031948:4856-4875 Msa1447900:CDS 45.0%
! TGAGTTGGGTGGAAGATTTG+AGG - tig0031948:4996-5015 None:intergenic 45.0%
!! TAGGACTAGGAGTGATGGAT+GGG - tig0031948:5034-5053 None:intergenic 45.0%
TGTAGTAGGACTAGGAGTGA+TGG - tig0031948:5039-5058 None:intergenic 45.0%
ACTCCTAGTCCTACTACAAC+AGG + tig0031948:5041-5060 Msa1447900:CDS 45.0%
ATTGATAGGTCCAGTTGGCA+CGG + tig0031948:5164-5183 Msa1447900:intron 45.0%
CACGGGTGAATGCTTCAAAA+GGG + tig0031948:5182-5201 Msa1447900:CDS 45.0%
AGCAGCAACAGCAACAACAA+TGG + tig0031948:4092-4111 Msa1447900:CDS 45.0%
TGGATCACCATCACAGTTTG+GGG + tig0031948:4112-4131 Msa1447900:CDS 45.0%
! CGACCATTGATGATCACAGT+TGG - tig0031948:4332-4351 None:intergenic 45.0%
AAGCGATATTGCAGTTGGCT+CGG - tig0031948:4355-4374 None:intergenic 45.0%
TTATGGGAGAAGACCTTGGA+CGG - tig0031948:4391-4410 None:intergenic 45.0%
GAAGTTATGGGAGAAGACCT+TGG - tig0031948:4395-4414 None:intergenic 45.0%
AATACCATTGAACCTGCTGC+TGG - tig0031948:4448-4467 None:intergenic 45.0%
GGTTCAATGGTATTATCCGG+TGG + tig0031948:4454-4473 Msa1447900:CDS 45.0%
! AATATTGGGGAAGGAGGTGT+GGG - tig0031948:4485-4504 None:intergenic 45.0%
TGATGGAATATTGGGGAAGG+AGG - tig0031948:4491-4510 None:intergenic 45.0%
CATATCTCTCTCTCTCTCTC+TGG - tig0031948:4592-4611 None:intergenic 45.0%
CAGAGAGAGAGAGAGAGATA+TGG + tig0031948:4590-4609 Msa1447900:intron 45.0%
CGAATGCAGAAACTGAGCTA+TGG + tig0031948:4846-4865 Msa1447900:intron 45.0%
GCAGAAACTGAGCTATGGAA+TGG + tig0031948:4851-4870 Msa1447900:intron 45.0%
AAACTGAGCTATGGAATGGG+TGG + tig0031948:4855-4874 Msa1447900:CDS 45.0%
AACTGAGCTATGGAATGGGT+GGG + tig0031948:4856-4875 Msa1447900:CDS 45.0%
! TGAGTTGGGTGGAAGATTTG+AGG - tig0031948:4996-5015 None:intergenic 45.0%
!! TAGGACTAGGAGTGATGGAT+GGG - tig0031948:5034-5053 None:intergenic 45.0%
TGTAGTAGGACTAGGAGTGA+TGG - tig0031948:5039-5058 None:intergenic 45.0%
ACTCCTAGTCCTACTACAAC+AGG + tig0031948:5041-5060 Msa1447900:CDS 45.0%
ATTGATAGGTCCAGTTGGCA+CGG + tig0031948:5164-5183 Msa1447900:intron 45.0%
CACGGGTGAATGCTTCAAAA+GGG + tig0031948:5182-5201 Msa1447900:CDS 45.0%
!!! TTAATTTTAATTTGAAAAAA+TGG + tig0031948:4818-4837 Msa1447900:intron 5.0%
!!! AGTTTAATTTATAAATAAAA+GGG - tig0031948:5080-5099 None:intergenic 5.0%
!!! AAGTTTAATTTATAAATAAA+AGG - tig0031948:5081-5100 None:intergenic 5.0%
!!! TTAATTTTAATTTGAAAAAA+TGG + tig0031948:4818-4837 Msa1447900:intron 5.0%
!!! AGTTTAATTTATAAATAAAA+GGG - tig0031948:5080-5099 None:intergenic 5.0%
!!! AAGTTTAATTTATAAATAAA+AGG - tig0031948:5081-5100 None:intergenic 5.0%
GTTGTGTCCCCAAACTGTGA+TGG - tig0031948:4122-4141 None:intergenic 50.0%
! TGTTTGTTGGAGGTCTTGCC+TGG + tig0031948:4153-4172 Msa1447900:CDS 50.0%
! GTTTGTTGGAGGTCTTGCCT+GGG + tig0031948:4154-4173 Msa1447900:CDS 50.0%
TGTGTCTTTCGGAGTTTCCC+AGG - tig0031948:4174-4193 None:intergenic 50.0%
AAGGTTACCGGCAGATCCAA+AGG + tig0031948:4251-4270 Msa1447900:CDS 50.0%
TCCATAGCCTTTGGATCTGC+CGG - tig0031948:4261-4280 None:intergenic 50.0%
AGATGCAGAGGCTGCAAAGA+AGG + tig0031948:4295-4314 Msa1447900:CDS 50.0%
GGAGGAGGAGGAGAAGTTAT+GGG - tig0031948:4407-4426 None:intergenic 50.0%
TGGAGGAGGAGGAGAAGTTA+TGG - tig0031948:4408-4427 None:intergenic 50.0%
GAATATTGGGGAAGGAGGTG+TGG - tig0031948:4486-4505 None:intergenic 50.0%
! AAGGAAGGGCGTGATGATGA+TGG - tig0031948:4508-4527 None:intergenic 50.0%
CATCACGCCCTTCCTTTCTA+CGG + tig0031948:4512-4531 Msa1447900:CDS 50.0%
ATCACGCCCTTCCTTTCTAC+GGG + tig0031948:4513-4532 Msa1447900:CDS 50.0%
ACTGAGCTATGGAATGGGTG+GGG + tig0031948:4857-4876 Msa1447900:CDS 50.0%
ATCTGTAGACAGGAGGAGCA+GGG - tig0031948:4941-4960 None:intergenic 50.0%
TCGGTTGATCTGTAGACAGG+AGG - tig0031948:4948-4967 None:intergenic 50.0%
TCCTGTCTACAGATCAACCG+AGG + tig0031948:4947-4966 Msa1447900:CDS 50.0%
CTACAGATCAACCGAGGCTA+TGG + tig0031948:4953-4972 Msa1447900:CDS 50.0%
! GGTGGAAGATTTGAGGTGGA+GGG - tig0031948:4989-5008 None:intergenic 50.0%
GTTGGGTGGAAGATTTGAGG+TGG - tig0031948:4993-5012 None:intergenic 50.0%
!! AGGACTAGGAGTGATGGATG+GGG - tig0031948:5033-5052 None:intergenic 50.0%
! GTAGGACTAGGAGTGATGGA+TGG - tig0031948:5035-5054 None:intergenic 50.0%
TTGATAGGTCCAGTTGGCAC+GGG + tig0031948:5165-5184 Msa1447900:intron 50.0%
GCACGGGTGAATGCTTCAAA+AGG + tig0031948:5181-5200 Msa1447900:CDS 50.0%
GTTGTGTCCCCAAACTGTGA+TGG - tig0031948:4122-4141 None:intergenic 50.0%
! TGTTTGTTGGAGGTCTTGCC+TGG + tig0031948:4153-4172 Msa1447900:CDS 50.0%
! GTTTGTTGGAGGTCTTGCCT+GGG + tig0031948:4154-4173 Msa1447900:CDS 50.0%
TGTGTCTTTCGGAGTTTCCC+AGG - tig0031948:4174-4193 None:intergenic 50.0%
AAGGTTACCGGCAGATCCAA+AGG + tig0031948:4251-4270 Msa1447900:CDS 50.0%
TCCATAGCCTTTGGATCTGC+CGG - tig0031948:4261-4280 None:intergenic 50.0%
AGATGCAGAGGCTGCAAAGA+AGG + tig0031948:4295-4314 Msa1447900:CDS 50.0%
GGAGGAGGAGGAGAAGTTAT+GGG - tig0031948:4407-4426 None:intergenic 50.0%
TGGAGGAGGAGGAGAAGTTA+TGG - tig0031948:4408-4427 None:intergenic 50.0%
GAATATTGGGGAAGGAGGTG+TGG - tig0031948:4486-4505 None:intergenic 50.0%
! AAGGAAGGGCGTGATGATGA+TGG - tig0031948:4508-4527 None:intergenic 50.0%
CATCACGCCCTTCCTTTCTA+CGG + tig0031948:4512-4531 Msa1447900:CDS 50.0%
ATCACGCCCTTCCTTTCTAC+GGG + tig0031948:4513-4532 Msa1447900:CDS 50.0%
ACTGAGCTATGGAATGGGTG+GGG + tig0031948:4857-4876 Msa1447900:CDS 50.0%
ATCTGTAGACAGGAGGAGCA+GGG - tig0031948:4941-4960 None:intergenic 50.0%
TCGGTTGATCTGTAGACAGG+AGG - tig0031948:4948-4967 None:intergenic 50.0%
TCCTGTCTACAGATCAACCG+AGG + tig0031948:4947-4966 Msa1447900:CDS 50.0%
CTACAGATCAACCGAGGCTA+TGG + tig0031948:4953-4972 Msa1447900:CDS 50.0%
! GGTGGAAGATTTGAGGTGGA+GGG - tig0031948:4989-5008 None:intergenic 50.0%
GTTGGGTGGAAGATTTGAGG+TGG - tig0031948:4993-5012 None:intergenic 50.0%
!! AGGACTAGGAGTGATGGATG+GGG - tig0031948:5033-5052 None:intergenic 50.0%
! GTAGGACTAGGAGTGATGGA+TGG - tig0031948:5035-5054 None:intergenic 50.0%
TTGATAGGTCCAGTTGGCAC+GGG + tig0031948:5165-5184 Msa1447900:intron 50.0%
GCACGGGTGAATGCTTCAAA+AGG + tig0031948:5181-5200 Msa1447900:CDS 50.0%
ACCGGCAGATCCAAAGGCTA+TGG + tig0031948:4257-4276 Msa1447900:CDS 55.0%
TTGCTGCGGTGGATCTTTGG+AGG - tig0031948:4425-4444 None:intergenic 55.0%
TGGTTGCTGCGGTGGATCTT+TGG - tig0031948:4428-4447 None:intergenic 55.0%
GCAACCAGCAGCAGGTTCAA+TGG + tig0031948:4441-4460 Msa1447900:CDS 55.0%
CTGAGCTATGGAATGGGTGG+GGG + tig0031948:4858-4877 Msa1447900:CDS 55.0%
! GCAGGGTGGTGGTACATGAT+TGG - tig0031948:4924-4943 None:intergenic 55.0%
GATCTGTAGACAGGAGGAGC+AGG - tig0031948:4942-4961 None:intergenic 55.0%
GCCTCGGTTGATCTGTAGAC+AGG - tig0031948:4951-4970 None:intergenic 55.0%
ATCAACCGAGGCTATGGCCA+TGG + tig0031948:4959-4978 Msa1447900:CDS 55.0%
TCAACCGAGGCTATGGCCAT+GGG + tig0031948:4960-4979 Msa1447900:CDS 55.0%
! GGGTGGAAGATTTGAGGTGG+AGG - tig0031948:4990-5009 None:intergenic 55.0%
AAGCATTCACCCGTGCCAAC+TGG - tig0031948:5177-5196 None:intergenic 55.0%
ACCGGCAGATCCAAAGGCTA+TGG + tig0031948:4257-4276 Msa1447900:CDS 55.0%
TTGCTGCGGTGGATCTTTGG+AGG - tig0031948:4425-4444 None:intergenic 55.0%
TGGTTGCTGCGGTGGATCTT+TGG - tig0031948:4428-4447 None:intergenic 55.0%
GCAACCAGCAGCAGGTTCAA+TGG + tig0031948:4441-4460 Msa1447900:CDS 55.0%
CTGAGCTATGGAATGGGTGG+GGG + tig0031948:4858-4877 Msa1447900:CDS 55.0%
! GCAGGGTGGTGGTACATGAT+TGG - tig0031948:4924-4943 None:intergenic 55.0%
GATCTGTAGACAGGAGGAGC+AGG - tig0031948:4942-4961 None:intergenic 55.0%
GCCTCGGTTGATCTGTAGAC+AGG - tig0031948:4951-4970 None:intergenic 55.0%
ATCAACCGAGGCTATGGCCA+TGG + tig0031948:4959-4978 Msa1447900:CDS 55.0%
TCAACCGAGGCTATGGCCAT+GGG + tig0031948:4960-4979 Msa1447900:CDS 55.0%
! GGGTGGAAGATTTGAGGTGG+AGG - tig0031948:4990-5009 None:intergenic 55.0%
AAGCATTCACCCGTGCCAAC+TGG - tig0031948:5177-5196 None:intergenic 55.0%
GGCTGCAAAGAAGGCTTGCG+AGG + tig0031948:4304-4323 Msa1447900:CDS 60.0%
! CGGTGGATCTTTGGAGGAGG+AGG - tig0031948:4419-4438 None:intergenic 60.0%
! CTGCGGTGGATCTTTGGAGG+AGG - tig0031948:4422-4441 None:intergenic 60.0%
! GAACCTGCTGCTGGTTGCTG+CGG - tig0031948:4439-4458 None:intergenic 60.0%
GCACCCACAATAGCCTGCCT+CGG - tig0031948:4897-4916 None:intergenic 60.0%
TGTAGACAGGAGGAGCAGGG+TGG - tig0031948:4938-4957 None:intergenic 60.0%
GACACCCATGGCCATAGCCT+CGG - tig0031948:4967-4986 None:intergenic 60.0%
TTGAGGTGGAGGGACACCCA+TGG - tig0031948:4979-4998 None:intergenic 60.0%
GGCTGCAAAGAAGGCTTGCG+AGG + tig0031948:4304-4323 Msa1447900:CDS 60.0%
! CGGTGGATCTTTGGAGGAGG+AGG - tig0031948:4419-4438 None:intergenic 60.0%
! CTGCGGTGGATCTTTGGAGG+AGG - tig0031948:4422-4441 None:intergenic 60.0%
! GAACCTGCTGCTGGTTGCTG+CGG - tig0031948:4439-4458 None:intergenic 60.0%
GCACCCACAATAGCCTGCCT+CGG - tig0031948:4897-4916 None:intergenic 60.0%
TGTAGACAGGAGGAGCAGGG+TGG - tig0031948:4938-4957 None:intergenic 60.0%
GACACCCATGGCCATAGCCT+CGG - tig0031948:4967-4986 None:intergenic 60.0%
TTGAGGTGGAGGGACACCCA+TGG - tig0031948:4979-4998 None:intergenic 60.0%
!! CTCTTGGTGCTCGCCGTCCA+AGG + tig0031948:4375-4394 Msa1447900:CDS 65.0%
! GGAGGTGTGGGTGTTCCCAC+CGG - tig0031948:4473-4492 None:intergenic 65.0%
CCACAATAGCCTGCCTCGGC+GGG - tig0031948:4893-4912 None:intergenic 65.0%
CCCACAATAGCCTGCCTCGG+CGG - tig0031948:4894-4913 None:intergenic 65.0%
CCGCCGAGGCAGGCTATTGT+GGG + tig0031948:4891-4910 Msa1447900:CDS 65.0%
AGACAGGAGGAGCAGGGTGG+TGG - tig0031948:4935-4954 None:intergenic 65.0%
!! CTCTTGGTGCTCGCCGTCCA+AGG + tig0031948:4375-4394 Msa1447900:CDS 65.0%
! GGAGGTGTGGGTGTTCCCAC+CGG - tig0031948:4473-4492 None:intergenic 65.0%
CCACAATAGCCTGCCTCGGC+GGG - tig0031948:4893-4912 None:intergenic 65.0%
CCCACAATAGCCTGCCTCGG+CGG - tig0031948:4894-4913 None:intergenic 65.0%
CCGCCGAGGCAGGCTATTGT+GGG + tig0031948:4891-4910 Msa1447900:CDS 65.0%
AGACAGGAGGAGCAGGGTGG+TGG - tig0031948:4935-4954 None:intergenic 65.0%
! CCTGCTGCTGGTTGCTGCGG+TGG - tig0031948:4436-4455 None:intergenic 70.0%
CCACCGCAGCAACCAGCAGC+AGG + tig0031948:4433-4452 Msa1447900:CDS 70.0%
CTACGTGTACCCGCCGAGGC+AGG + tig0031948:4881-4900 Msa1447900:CDS 70.0%
CCCGCCGAGGCAGGCTATTG+TGG + tig0031948:4890-4909 Msa1447900:CDS 70.0%
! CCTGCTGCTGGTTGCTGCGG+TGG - tig0031948:4436-4455 None:intergenic 70.0%
CCACCGCAGCAACCAGCAGC+AGG + tig0031948:4433-4452 Msa1447900:CDS 70.0%
CTACGTGTACCCGCCGAGGC+AGG + tig0031948:4881-4900 Msa1447900:CDS 70.0%
CCCGCCGAGGCAGGCTATTG+TGG + tig0031948:4890-4909 Msa1447900:CDS 70.0%
GGGGCTACGTGTACCCGCCG+AGG + tig0031948:4877-4896 Msa1447900:CDS 75.0%
GGGGCTACGTGTACCCGCCG+AGG + tig0031948:4877-4896 Msa1447900:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0031948 gene 3963 5215 3963 ID=Msa1447900;Name=Msa1447900
tig0031948 mRNA 3963 5215 3963 ID=Msa1447900-mRNA-1;Parent=Msa1447900;Name=Msa1447900-mRNA-1;_AED=0.03;_eAED=0.03;_QI=114|0.66|0.5|1|1|1|4|0|255
tig0031948 exon 3963 4534 3963 ID=Msa1447900-mRNA-1:exon:16126;Parent=Msa1447900-mRNA-1
tig0031948 exon 4699 4756 4699 ID=Msa1447900-mRNA-1:exon:16127;Parent=Msa1447900-mRNA-1
tig0031948 exon 4855 5062 4855 ID=Msa1447900-mRNA-1:exon:16128;Parent=Msa1447900-mRNA-1
tig0031948 exon 5172 5215 5172 ID=Msa1447900-mRNA-1:exon:16129;Parent=Msa1447900-mRNA-1
tig0031948 five_prime_UTR 3963 4076 3963 ID=Msa1447900-mRNA-1:five_prime_utr;Parent=Msa1447900-mRNA-1
tig0031948 CDS 4077 4534 4077 ID=Msa1447900-mRNA-1:cds;Parent=Msa1447900-mRNA-1
tig0031948 CDS 4699 4756 4699 ID=Msa1447900-mRNA-1:cds;Parent=Msa1447900-mRNA-1
tig0031948 CDS 4855 5062 4855 ID=Msa1447900-mRNA-1:cds;Parent=Msa1447900-mRNA-1
tig0031948 CDS 5172 5215 5172 ID=Msa1447900-mRNA-1:cds;Parent=Msa1447900-mRNA-1
Gene Sequence

>Msa1447900

ATGACGATGATGGGCAGCAGCAACAGCAACAACAATGGATCACCATCACAGTTTGGGGACACAACGCTGACAAAAGTGTTTGTTGGAGGTCTTGCCTGGGAAACTCCGAAAGACACATTAAGAGAACACTTTGAAAAATACGGAGACATCCTTGAAGCTGTCATAATTTCTGATAAGGTTACCGGCAGATCCAAAGGCTATGGATTTGTAACATTCAAAGATGCAGAGGCTGCAAAGAAGGCTTGCGAGGAACCAACTGTGATCATCAATGGTCGCCGAGCCAACTGCAATATCGCTTCTCTTGGTGCTCGCCGTCCAAGGTCTTCTCCCATAACTTCTCCTCCTCCTCCAAAGATCCACCGCAGCAACCAGCAGCAGGTTCAATGGTATTATCCGGTGGGAACACCCACACCTCCTTCCCCAATATTCCATCATCATCACGCCCTTCCTTTCTACGGGTACACGCCCACCTACATTGCAACAGATCATATGAATTACAACTGCAATTTCAATCAGAAACTGAGCTATGGAATGGGTGGGGGCTACGTGTACCCGCCGAGGCAGGCTATTGTGGGTGCGATGCCAATCATGTACCACCACCCTGCTCCTCCTGTCTACAGATCAACCGAGGCTATGGCCATGGGTGTCCCTCCACCTCAAATCTTCCACCCAACTCATCATTATCATTATTCCCCATCCATCACTCCTAGTCCTACTACAACAGGTCCAGTTGGCACGGGTGAATGCTTCAAAAGGGTAGTGGTCTGA
Protein sequence

>Msa1447900

MTMMGSSNSNNNGSPSQFGDTTLTKVFVGGLAWETPKDTLREHFEKYGDILEAVIISDKVTGRSKGYGFVTFKDAEAAKKACEEPTVIINGRRANCNIASLGARRPRSSPITSPPPPKIHRSNQQQVQWYYPVGTPTPPSPIFHHHHALPFYGYTPTYIATDHMNYNCNFNQKLSYGMGGGYVYPPRQAIVGAMPIMYHHPAPPVYRSTEAMAMGVPPPQIFHPTHHYHYSPSITPSPTTTGPVGTGECFKRVVV*