AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1417950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417950 MtrunA17_Chr7g0276531 83.895 267 20 7 3 257 1 256 7.41e-142 398
Msa1417950 MtrunA17_Chr8g0346011 67.010 97 32 0 12 108 25 121 9.75e-44 149
Msa1417950 MtrunA17_Chr5g0426311 74.419 86 22 0 16 101 8 93 1.25e-43 149
Msa1417950 MtrunA17_Chr3g0144311 69.072 97 28 1 16 112 21 115 4.37e-43 147
Msa1417950 MtrunA17_Chr4g0017401 66.667 93 31 0 17 109 9 101 2.05e-41 142
Msa1417950 MtrunA17_Chr3g0127751 50.382 131 55 2 12 132 14 144 9.71e-38 132
Msa1417950 MtrunA17_Chr4g0045921 48.810 84 43 0 20 103 72 155 2.19e-19 86.3
Msa1417950 MtrunA17_Chr4g0045921 32.584 89 59 1 19 107 158 245 8.52e-12 64.3
Msa1417950 MtrunA17_Chr7g0255011 44.048 84 47 0 19 102 122 205 5.50e-18 82.8
Msa1417950 MtrunA17_Chr7g0266751 37.391 115 69 2 10 121 16 130 5.55e-18 78.2
Msa1417950 MtrunA17_Chr2g0290191 47.059 68 35 1 25 92 5 71 9.73e-18 82.0
Msa1417950 MtrunA17_Chr8g0360551 44.578 83 46 0 20 102 69 151 1.22e-17 81.6
Msa1417950 MtrunA17_Chr8g0360551 37.363 91 57 0 9 99 150 240 3.06e-13 68.9
Msa1417950 MtrunA17_Chr2g0278661 48.649 74 37 1 20 92 3 76 1.31e-17 79.7
Msa1417950 MtrunA17_Chr3g0101061 42.105 76 43 1 17 92 33 107 6.22e-17 79.7
Msa1417950 MtrunA17_Chr4g0061451 43.529 85 44 2 24 107 42 123 9.64e-17 77.8
Msa1417950 MtrunA17_Chr7g0219021 43.210 81 45 1 25 104 41 121 2.34e-16 76.3
Msa1417950 MtrunA17_Chr3g0121631 43.478 69 39 0 24 92 36 104 3.56e-16 73.6
Msa1417950 MtrunA17_Chr3g0086861 42.857 91 48 2 18 107 38 125 9.50e-16 76.3
Msa1417950 MtrunA17_Chr4g0035051 49.153 59 30 0 25 83 9 67 9.57e-16 71.6
Msa1417950 MtrunA17_Chr5g0440831 39.744 78 47 0 25 102 166 243 2.94e-15 75.1
Msa1417950 MtrunA17_Chr4g0050781 45.833 72 35 1 20 91 3 70 7.49e-15 71.2
Msa1417950 MtrunA17_Chr7g0219031 49.153 59 30 0 25 83 41 99 8.58e-15 68.9
Msa1417950 MtrunA17_Chr5g0447761 46.667 60 32 0 25 84 7 66 9.65e-15 73.6
Msa1417950 MtrunA17_Chr4g0035031 43.421 76 42 1 25 99 9 84 9.79e-15 70.5
Msa1417950 MtrunA17_Chr1g0186351 37.500 80 49 1 20 99 2 80 1.04e-14 73.2
Msa1417950 MtrunA17_Chr3g0119311 36.957 92 54 2 24 114 36 124 1.09e-14 69.3
Msa1417950 MtrunA17_Chr4g0035001 47.458 59 31 0 25 83 7 65 3.24e-14 68.6
Msa1417950 MtrunA17_Chr3g0135111 42.500 80 45 1 20 99 6 84 3.27e-14 71.6
Msa1417950 MtrunA17_Chr4g0031091 45.070 71 38 1 24 93 33 103 2.30e-13 65.1
Msa1417950 MtrunA17_Chr1g0198431 37.755 98 60 1 3 99 171 268 3.27e-13 67.8
Msa1417950 MtrunA17_Chr1g0210241 36.905 84 49 1 24 107 7 86 3.12e-12 65.1
Msa1417950 MtrunA17_Chr4g0042161 45.000 60 33 0 24 83 51 110 3.74e-12 62.4
Msa1417950 MtrunA17_Chr3g0088511 44.828 58 32 0 24 81 32 89 7.82e-12 61.2
Msa1417950 MtrunA17_Chr3g0120721 40.625 64 38 0 25 88 19 82 9.67e-12 64.3
Msa1417950 MtrunA17_Chr6g0449481 42.188 64 37 0 25 88 46 109 3.29e-11 62.8
Msa1417950 MtrunA17_Chr7g0276201 47.541 61 32 0 24 84 104 164 5.07e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417950 AT3G54770.1 46.429 252 110 8 6 246 2 239 4.75e-54 175
Msa1417950 AT3G54770.3 46.429 252 110 8 6 246 2 239 7.19e-54 175
Msa1417950 AT1G20880.2 75.824 91 22 0 16 106 16 106 1.07e-45 154
Msa1417950 AT1G20880.3 75.824 91 22 0 16 106 16 106 1.07e-45 154
Msa1417950 AT1G20880.1 75.824 91 22 0 16 106 16 106 1.07e-45 154
Msa1417950 AT1G76460.1 75.824 91 22 0 16 106 16 106 1.08e-45 154
Msa1417950 AT1G22910.2 67.391 92 30 0 18 109 7 98 1.63e-41 142
Msa1417950 AT1G22910.1 67.391 92 30 0 18 109 7 98 2.33e-41 142
Msa1417950 AT2G46780.1 63.810 105 31 2 10 114 13 110 7.91e-41 143
Msa1417950 AT2G46780.2 63.810 105 31 2 10 114 13 110 7.91e-41 143
Msa1417950 AT1G22910.3 67.391 92 30 0 18 109 7 98 1.86e-40 142
Msa1417950 AT1G33470.3 67.742 93 29 1 20 111 3 95 8.46e-40 138
Msa1417950 AT1G33470.1 67.742 93 29 1 20 111 3 95 9.41e-40 138
Msa1417950 AT1G33470.2 67.742 93 29 1 20 111 3 95 9.46e-40 138
Msa1417950 AT3G54770.2 45.070 213 94 8 40 246 1 196 1.02e-39 137
Msa1417950 AT1G22330.1 65.934 91 31 0 16 106 9 99 1.35e-39 139
Msa1417950 AT1G78260.1 66.279 86 29 0 16 101 9 94 1.08e-37 134
Msa1417950 AT1G78260.2 66.279 86 29 0 16 101 9 94 1.52e-37 133
Msa1417950 AT5G11412.1 54.118 85 39 0 20 104 48 132 1.40e-29 110
Msa1417950 AT5G53680.1 50.588 85 42 0 20 104 9 93 2.43e-26 101
Msa1417950 AT3G06970.1 46.154 91 49 0 20 110 8 98 6.57e-24 98.6
Msa1417950 AT5G53720.1 49.383 81 41 0 24 104 9 89 1.44e-23 92.0
Msa1417950 AT2G22090.2 49.398 83 42 0 20 102 100 182 1.71e-19 86.7
Msa1417950 AT2G22090.1 49.398 83 42 0 20 102 100 182 1.73e-19 86.7
Msa1417950 AT3G15010.1 37.500 96 60 0 9 104 152 247 1.77e-18 84.3
Msa1417950 AT3G15010.1 46.988 83 44 0 20 102 71 153 1.05e-17 82.0
Msa1417950 AT3G15010.2 37.500 96 60 0 9 104 152 247 1.77e-18 84.3
Msa1417950 AT3G15010.2 46.988 83 44 0 20 102 71 153 1.05e-17 82.0
Msa1417950 AT3G23830.2 41.379 87 50 1 24 109 35 121 3.14e-18 79.0
Msa1417950 AT3G23830.1 41.379 87 50 1 24 109 35 121 3.14e-18 79.0
Msa1417950 AT4G39260.3 40.000 85 47 1 25 109 7 87 5.22e-18 77.0
Msa1417950 AT4G26650.2 44.444 72 39 1 21 92 9 79 2.39e-17 81.3
Msa1417950 AT4G26650.1 44.444 72 39 1 21 92 12 82 2.48e-17 81.3
Msa1417950 AT2G41060.1 36.792 106 61 2 20 121 124 227 6.36e-17 80.1
Msa1417950 AT2G41060.3 36.792 106 61 2 20 121 124 227 6.36e-17 80.1
Msa1417950 AT2G41060.2 36.792 106 61 2 20 121 124 227 6.36e-17 80.1
Msa1417950 AT4G39260.4 50.000 60 30 0 25 84 7 66 7.01e-17 74.3
Msa1417950 AT4G39260.2 41.558 77 41 1 25 101 7 79 7.90e-17 75.1
Msa1417950 AT2G19380.1 30.220 182 102 5 20 182 404 579 1.27e-16 79.3
Msa1417950 AT4G14300.1 44.928 69 38 0 20 88 2 70 1.41e-16 79.0
Msa1417950 AT4G14300.2 44.928 69 38 0 20 88 2 70 1.41e-16 79.0
Msa1417950 AT4G39260.1 41.558 77 41 1 25 101 7 79 2.16e-16 75.1
Msa1417950 AT5G55550.5 41.096 73 42 1 20 92 2 73 2.68e-16 78.2
Msa1417950 AT5G55550.4 41.096 73 42 1 20 92 2 73 2.68e-16 78.2
Msa1417950 AT5G55550.9 41.096 73 42 1 20 92 2 73 2.68e-16 78.2
Msa1417950 AT5G55550.7 41.096 73 42 1 20 92 2 73 2.68e-16 78.2
Msa1417950 AT5G55550.1 41.096 73 42 1 20 92 2 73 2.68e-16 78.2
Msa1417950 AT4G13850.4 45.333 75 40 1 24 97 35 109 2.72e-16 73.6
Msa1417950 AT2G21660.2 54.237 59 27 0 25 83 9 67 2.81e-16 74.3
Msa1417950 AT5G55550.8 41.096 73 42 1 20 92 2 73 2.90e-16 78.2
Msa1417950 AT5G55550.2 41.096 73 42 1 20 92 2 73 3.02e-16 78.2
Msa1417950 AT5G55550.3 41.096 73 42 1 20 92 2 73 3.02e-16 78.2
Msa1417950 AT5G55550.6 41.096 73 42 1 20 92 2 73 3.02e-16 78.2
Msa1417950 AT5G55550.11 41.096 73 42 1 20 92 2 73 3.02e-16 78.2
Msa1417950 AT5G55550.10 41.096 73 42 1 20 92 2 73 3.24e-16 78.2
Msa1417950 AT4G13850.3 45.333 75 40 1 24 97 35 109 3.48e-16 73.9
Msa1417950 AT2G21660.1 54.237 59 27 0 25 83 9 67 4.05e-16 74.3
Msa1417950 AT4G13850.2 45.333 75 40 1 24 97 35 109 4.28e-16 73.9
Msa1417950 AT5G47620.1 48.276 58 30 0 25 82 7 64 4.65e-16 77.4
Msa1417950 AT5G47620.2 48.276 58 30 0 25 82 7 64 4.65e-16 77.4
Msa1417950 AT5G47620.5 48.276 58 30 0 25 82 7 64 4.65e-16 77.4
Msa1417950 AT4G13850.1 45.333 75 40 1 24 97 35 109 4.79e-16 73.9
Msa1417950 AT3G56860.9 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.11 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.8 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.5 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.7 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.2 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.6 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.10 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.3 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.1 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT3G56860.4 42.308 78 45 0 25 102 141 218 4.97e-16 77.4
Msa1417950 AT5G47620.4 48.276 58 30 0 25 82 7 64 5.72e-16 77.4
Msa1417950 AT1G17640.2 29.771 131 80 2 25 147 67 193 1.13e-15 76.3
Msa1417950 AT1G17640.1 29.771 131 80 2 25 147 67 193 1.13e-15 76.3
Msa1417950 AT1G17640.3 29.771 131 80 2 25 147 67 193 1.13e-15 76.3
Msa1417950 AT1G74230.1 44.156 77 43 0 24 100 34 110 2.24e-15 74.3
Msa1417950 AT2G22100.1 35.135 111 69 1 20 127 159 269 2.43e-15 75.1
Msa1417950 AT3G26420.1 47.761 67 31 1 25 91 8 70 2.95e-15 73.6
Msa1417950 AT5G61030.2 44.928 69 34 1 24 92 40 104 2.97e-15 74.3
Msa1417950 AT5G61030.1 44.928 69 34 1 24 92 40 104 2.97e-15 74.3
Msa1417950 AT3G07810.1 34.615 104 56 3 25 128 7 98 2.03e-14 72.8
Msa1417950 AT5G06210.1 45.833 72 35 1 24 95 34 101 2.07e-14 68.9
Msa1417950 AT3G07810.2 34.615 104 56 3 25 128 7 98 2.22e-14 72.8
Msa1417950 AT4G13860.1 39.024 82 45 2 24 102 3 82 1.52e-13 65.1
Msa1417950 AT3G13224.2 39.362 94 52 3 4 96 1 90 3.69e-13 68.9
Msa1417950 AT5G59865.1 50.980 51 25 0 22 72 24 74 1.43e-12 63.2
Msa1417950 AT2G33410.1 33.803 71 47 0 20 90 2 72 1.53e-12 67.0
Msa1417950 AT3G13224.1 39.362 94 52 3 4 96 1 90 1.77e-12 65.5
Msa1417950 AT3G08000.1 33.654 104 61 3 25 125 42 140 2.70e-12 63.2
Msa1417950 AT1G58470.2 41.429 70 41 0 20 89 2 71 3.04e-12 66.2
Msa1417950 AT1G58470.1 41.429 70 41 0 20 89 2 71 3.04e-12 66.2
Msa1417950 AT5G40490.1 44.615 65 36 0 25 89 43 107 5.56e-12 65.5
Msa1417950 AT3G13224.3 40.845 71 41 1 25 95 11 80 9.49e-12 64.7
Msa1417950 AT3G20930.1 40.909 88 50 2 25 110 283 370 1.18e-11 64.3
Msa1417950 AT1G18630.1 41.791 67 37 1 19 83 29 95 1.44e-11 61.6
Msa1417950 AT4G36960.1 40.678 59 35 0 25 83 4 62 4.46e-11 62.8
Msa1417950 AT4G36960.2 40.678 59 35 0 25 83 4 62 4.46e-11 62.8
Msa1417950 AT4G20030.1 36.458 96 60 1 24 118 40 135 6.07e-11 59.7

Find 112 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCAGTAACAAAAGTATT+TGG 0.196601 tig0024531:-56110 None:intergenic
TGATGATGATGATGGAATAT+TGG 0.313706 tig0024531:-56644 None:intergenic
GATGATGATGATGGAATATT+GGG 0.327379 tig0024531:-56643 None:intergenic
ATGGATCACCATCACAGTTT+GGG 0.341928 tig0024531:+56258 Msa1417950:CDS
AATGGATCACCATCACAGTT+TGG 0.360186 tig0024531:+56257 Msa1417950:CDS
TGTTCTCTTAATGTGTCTTT+CGG 0.360765 tig0024531:-56329 None:intergenic
GGAGGAGGAGGCGAAGTTAT+GGG 0.369492 tig0024531:-56551 None:intergenic
TGGTTGCTGCGGTGGATCTT+TGG 0.373820 tig0024531:-56572 None:intergenic
ATAATTTCTGATAAGGTTAC+CGG 0.382398 tig0024531:+56386 Msa1417950:CDS
TGTTTGTTGGAGGTCTTGCC+TGG 0.388391 tig0024531:+56300 Msa1417950:CDS
AGCTGTCATAATTTCTGATA+AGG 0.401698 tig0024531:+56379 Msa1417950:CDS
AAGCATTCACCCGTGCCAAC+TGG 0.407073 tig0024531:-57332 None:intergenic
CATCACGCCCTTCCTTTCTA+CGG 0.413597 tig0024531:+56665 Msa1417950:CDS
CCTGCTGCTGGTTGCTGCGG+TGG 0.413826 tig0024531:-56580 None:intergenic
ACTCAGTAACAAAAGTATTT+GGG 0.422425 tig0024531:-56109 None:intergenic
CTCTTGGTGCTCGCCGTCCA+AGG 0.426692 tig0024531:+56522 Msa1417950:CDS
AATAATGATAATGATGAGTT+GGG 0.437625 tig0024531:-57165 None:intergenic
TGGAGGAGGAGGCGAAGTTA+TGG 0.450266 tig0024531:-56552 None:intergenic
TGTTACAAATCCATACCCTT+TGG 0.450417 tig0024531:-56414 None:intergenic
CAGAAATTATGACAGCTTCA+AGG 0.453018 tig0024531:-56373 None:intergenic
CCGCCGAGGCAGGCTATTGT+GGG 0.455950 tig0024531:+57049 Msa1417950:CDS
ACGCTGACAAAAGTGTTTGT+TGG 0.457047 tig0024531:+56287 Msa1417950:CDS
TCCATACCCTTTGGATCTGC+CGG 0.471487 tig0024531:-56405 None:intergenic
GAATAATGATAATGATGAGT+TGG 0.476228 tig0024531:-57166 None:intergenic
GATCTGTAGACAGGAGGAGC+AGG 0.479037 tig0024531:-57097 None:intergenic
GAATATTGGGGAAGGAGGTG+TGG 0.480515 tig0024531:-56630 None:intergenic
GCACGGGTGAATGCTTCAAA+AGG 0.482053 tig0024531:+57339 Msa1417950:CDS
ACTCCTAGTCCTACTACAAC+AGG 0.483068 tig0024531:+57199 Msa1417950:CDS
GGGTGGAAGATTTGAGGTGG+AGG 0.485695 tig0024531:-57145 None:intergenic
AGATGCAGAGGCTGCAAAGA+AGG 0.488455 tig0024531:+56442 Msa1417950:CDS
TGTGTCTTTCGGAGTTTCCC+AGG 0.489644 tig0024531:-56318 None:intergenic
CCACAATAGCCTGCCTCGGC+GGG 0.493685 tig0024531:-57048 None:intergenic
GCAGGGTGGTGGTACATGAT+TGG 0.499499 tig0024531:-57079 None:intergenic
ATCACGCCCTTCCTTTCTAC+GGG 0.499631 tig0024531:+56666 Msa1417950:CDS
CCACCGCAGCAACCAGCAGC+AGG 0.500038 tig0024531:+56580 Msa1417950:CDS
ATCAACCGAGGCTATGGCCA+TGG 0.501706 tig0024531:+57117 Msa1417950:CDS
CGGTGGATCTTTGGAGGAGG+AGG 0.505079 tig0024531:-56563 None:intergenic
ATATATTAACCTGTTGTAGT+AGG 0.505308 tig0024531:-57208 None:intergenic
CTACAGATCAACCGAGGCTA+TGG 0.506662 tig0024531:+57111 Msa1417950:CDS
TTGCTGCGGTGGATCTTTGG+AGG 0.509090 tig0024531:-56569 None:intergenic
CTGAGCTATGGAATGGGTGG+GGG 0.510253 tig0024531:+57016 Msa1417950:CDS
TAAAATATATACCCGTAGAA+AGG 0.515467 tig0024531:-56677 None:intergenic
CATATGATCTGTTGCAATGT+AGG 0.515946 tig0024531:-56868 None:intergenic
GCCTCGGTTGATCTGTAGAC+AGG 0.521868 tig0024531:-57106 None:intergenic
ACCGGCAGATCCAAAGGGTA+TGG 0.523127 tig0024531:+56404 Msa1417950:CDS
ATATATACCCGTAGAAAGGA+AGG 0.523229 tig0024531:-56673 None:intergenic
AACTGCAATATCGCTTCTCT+TGG 0.523354 tig0024531:+56506 Msa1417950:CDS
TAGTCAGTCTATAAGAAAGA+AGG 0.524612 tig0024531:-56162 None:intergenic
GCAGGTTCAATGGTATTATC+CGG 0.525480 tig0024531:+56598 Msa1417950:CDS
AACTGAGCTATGGAATGGGT+GGG 0.529212 tig0024531:+57014 Msa1417950:CDS
TAACCTGTTGTAGTAGGACT+AGG 0.534126 tig0024531:-57202 None:intergenic
TAGGACTAGGAGTGATGGAT+GGG 0.535767 tig0024531:-57189 None:intergenic
TTACAACTGCAATTACAATC+AGG 0.535938 tig0024531:+56893 Msa1417950:CDS
GGAGGTGTGGGTGTTCCCAC+CGG 0.538024 tig0024531:-56617 None:intergenic
AGACAGGAGGAGCAGGGTGG+TGG 0.540978 tig0024531:-57090 None:intergenic
CTGACAAAAGTGTTTGTTGG+AGG 0.542823 tig0024531:+56290 Msa1417950:CDS
GCAACCAGCAGCAGGTTCAA+TGG 0.542889 tig0024531:+56588 Msa1417950:CDS
GACACCCATGGCCATAGCCT+CGG 0.543694 tig0024531:-57122 None:intergenic
ACTGAGCTATGGAATGGGTG+GGG 0.544490 tig0024531:+57015 Msa1417950:CDS
AATATTGGGGAAGGAGGTGT+GGG 0.546556 tig0024531:-56629 None:intergenic
CTGCGGTGGATCTTTGGAGG+AGG 0.548182 tig0024531:-56566 None:intergenic
GATATTGATCATGACGATGA+TGG 0.548742 tig0024531:+56214 Msa1417950:exon
GAACCAACTGTGATCATCAA+TGG 0.551058 tig0024531:+56473 Msa1417950:CDS
ATTGATAGGTCCAGTTGGCA+CGG 0.551120 tig0024531:+57322 Msa1417950:intron
AATACCATTGAACCTGCTGC+TGG 0.553977 tig0024531:-56592 None:intergenic
AAGGTTACCGGCAGATCCAA+AGG 0.555394 tig0024531:+56398 Msa1417950:CDS
AAGCGATATTGCAGTTGGCT+CGG 0.556799 tig0024531:-56499 None:intergenic
TTGATAGGTCCAGTTGGCAC+GGG 0.560846 tig0024531:+57323 Msa1417950:intron
CGAATGCAGAAACTGAGCTA+TGG 0.568591 tig0024531:+57004 Msa1417950:intron
CCCACAATAGCCTGCCTCGG+CGG 0.568725 tig0024531:-57049 None:intergenic
AAACTGAGCTATGGAATGGG+TGG 0.571141 tig0024531:+57013 Msa1417950:CDS
GTAGGACTAGGAGTGATGGA+TGG 0.572210 tig0024531:-57190 None:intergenic
GAACCTGCTGCTGGTTGCTG+CGG 0.576807 tig0024531:-56583 None:intergenic
CGACCATTGATGATCACAGT+TGG 0.578434 tig0024531:-56476 None:intergenic
TCAACCGAGGCTATGGCCAT+GGG 0.585523 tig0024531:+57118 Msa1417950:CDS
GGTGGAAGATTTGAGGTGGA+GGG 0.586269 tig0024531:-57144 None:intergenic
TGAGTTGGGTGGAAGATTTG+AGG 0.586506 tig0024531:-57151 None:intergenic
TGATGGAATATTGGGGAAGG+AGG 0.587373 tig0024531:-56635 None:intergenic
TGAATGCTTCAAAAGGGTAG+TGG 0.590972 tig0024531:+57346 Msa1417950:CDS
GGCTGCAAAGAAGGCTTGCG+AGG 0.592252 tig0024531:+56451 Msa1417950:CDS
TGAATATTGATAGGTCCAGT+TGG 0.592422 tig0024531:+57317 Msa1417950:intron
GCAGAAACTGAGCTATGGAA+TGG 0.594468 tig0024531:+57009 Msa1417950:intron
AAGAGAAGCGATATTGCAGT+TGG 0.596190 tig0024531:-56504 None:intergenic
TGATGATGGAATATTGGGGA+AGG 0.597893 tig0024531:-56638 None:intergenic
TGTAGTAGGACTAGGAGTGA+TGG 0.600167 tig0024531:-57194 None:intergenic
GTTGGGTGGAAGATTTGAGG+TGG 0.600463 tig0024531:-57148 None:intergenic
GCACCCACAATAGCCTGCCT+CGG 0.602284 tig0024531:-57052 None:intergenic
GAAGTTATGGGAGAAGACCT+TGG 0.602826 tig0024531:-56539 None:intergenic
ATGATCTGTTGCAATGTAGG+TGG 0.608013 tig0024531:-56865 None:intergenic
GGTTCAATGGTATTATCCGG+TGG 0.611241 tig0024531:+56601 Msa1417950:CDS
CAGAAACTGAGCTATGGAAT+GGG 0.612059 tig0024531:+57010 Msa1417950:intron
TGTAACATTCAAAGATGCAG+AGG 0.612207 tig0024531:+56430 Msa1417950:CDS
CACGGGTGAATGCTTCAAAA+GGG 0.613875 tig0024531:+57340 Msa1417950:CDS
ATATTGATCATGACGATGAT+GGG 0.616366 tig0024531:+56215 Msa1417950:exon
AGGTTACCGGCAGATCCAAA+GGG 0.624786 tig0024531:+56399 Msa1417950:CDS
TTGAGGTGGAGGGACACCCA+TGG 0.627473 tig0024531:-57134 None:intergenic
TATATACCCGTAGAAAGGAA+GGG 0.631562 tig0024531:-56672 None:intergenic
ATCTGTAGACAGGAGGAGCA+GGG 0.632459 tig0024531:-57096 None:intergenic
GTTTGTTGGAGGTCTTGCCT+GGG 0.633552 tig0024531:+56301 Msa1417950:CDS
GTTCAATGGTATTATCCGGT+GGG 0.634469 tig0024531:+56602 Msa1417950:CDS
TGATCTGTTGCAATGTAGGT+GGG 0.635848 tig0024531:-56864 None:intergenic
AATGATAATGATGAGTTGGG+TGG 0.637114 tig0024531:-57162 None:intergenic
TTATGGGAGAAGACCTTGGA+CGG 0.649901 tig0024531:-56535 None:intergenic
GTTGTGTCCCCAAACTGTGA+TGG 0.652121 tig0024531:-56266 None:intergenic
AGCAGCAACAGCAACAACAA+TGG 0.659160 tig0024531:+56239 Msa1417950:CDS
GGGGCTACGTGTACCCGCCG+AGG 0.659428 tig0024531:+57035 Msa1417950:CDS
AGGACTAGGAGTGATGGATG+GGG 0.665517 tig0024531:-57188 None:intergenic
TGGATCACCATCACAGTTTG+GGG 0.666646 tig0024531:+56259 Msa1417950:CDS
TCGGTTGATCTGTAGACAGG+AGG 0.670555 tig0024531:-57103 None:intergenic
ATGATGATGATGGAATATTG+GGG 0.703347 tig0024531:-56642 None:intergenic
TCCTGTCTACAGATCAACCG+AGG 0.712533 tig0024531:+57105 Msa1417950:CDS
TGTAGACAGGAGGAGCAGGG+TGG 0.747299 tig0024531:-57093 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTCTTGTTATTGAATT+AGG - tig0024531:56955-56974 None:intergenic 15.0%
!!! TTTTAATTTGAAAAAATGGT+TGG + tig0024531:56980-56999 Msa1417950:intron 15.0%
!!! ATATTTCTTGTTATTGAATT+AGG - tig0024531:56955-56974 None:intergenic 15.0%
!!! TTTTAATTTGAAAAAATGGT+TGG + tig0024531:56980-56999 Msa1417950:intron 15.0%
!!! CATTTAAATCACATTTGTTA+AGG - tig0024531:56814-56833 None:intergenic 20.0%
!!! AATAATGATAATGATGAGTT+GGG - tig0024531:57168-57187 None:intergenic 20.0%
!!! CATTTAAATCACATTTGTTA+AGG - tig0024531:56814-56833 None:intergenic 20.0%
!!! AATAATGATAATGATGAGTT+GGG - tig0024531:57168-57187 None:intergenic 20.0%
!! AGAGAACACTTTGAAAAATA+CGG + tig0024531:56344-56363 Msa1417950:CDS 25.0%
! ATAATTTCTGATAAGGTTAC+CGG + tig0024531:56386-56405 Msa1417950:CDS 25.0%
! TAAAATATATACCCGTAGAA+AGG - tig0024531:56680-56699 None:intergenic 25.0%
!! TAAATCACATTTGTTAAGGT+CGG - tig0024531:56810-56829 None:intergenic 25.0%
!! GAATAATGATAATGATGAGT+TGG - tig0024531:57169-57188 None:intergenic 25.0%
! ATATATTAACCTGTTGTAGT+AGG - tig0024531:57211-57230 None:intergenic 25.0%
!! AGAGAACACTTTGAAAAATA+CGG + tig0024531:56344-56363 Msa1417950:CDS 25.0%
! ATAATTTCTGATAAGGTTAC+CGG + tig0024531:56386-56405 Msa1417950:CDS 25.0%
! TAAAATATATACCCGTAGAA+AGG - tig0024531:56680-56699 None:intergenic 25.0%
!! TAAATCACATTTGTTAAGGT+CGG - tig0024531:56810-56829 None:intergenic 25.0%
!! GAATAATGATAATGATGAGT+TGG - tig0024531:57169-57188 None:intergenic 25.0%
! ATATATTAACCTGTTGTAGT+AGG - tig0024531:57211-57230 None:intergenic 25.0%
!! CACTCAGTAACAAAAGTATT+TGG - tig0024531:56113-56132 None:intergenic 30.0%
TAGTCAGTCTATAAGAAAGA+AGG - tig0024531:56165-56184 None:intergenic 30.0%
ATATTGATCATGACGATGAT+GGG + tig0024531:56215-56234 Msa1417950:exon 30.0%
TGTTCTCTTAATGTGTCTTT+CGG - tig0024531:56332-56351 None:intergenic 30.0%
AGCTGTCATAATTTCTGATA+AGG + tig0024531:56379-56398 Msa1417950:CDS 30.0%
!! ATGATGATGATGGAATATTG+GGG - tig0024531:56645-56664 None:intergenic 30.0%
!! GATGATGATGATGGAATATT+GGG - tig0024531:56646-56665 None:intergenic 30.0%
!! TGATGATGATGATGGAATAT+TGG - tig0024531:56647-56666 None:intergenic 30.0%
! AAATCACATTTGTTAAGGTC+GGG - tig0024531:56809-56828 None:intergenic 30.0%
TATACCTGAATGAAAGAAAG+AGG - tig0024531:56842-56861 None:intergenic 30.0%
TTACAACTGCAATTACAATC+AGG + tig0024531:56893-56912 Msa1417950:CDS 30.0%
GGCATGCAATGTAATAATAT+TGG - tig0024531:56934-56953 None:intergenic 30.0%
!! CACTCAGTAACAAAAGTATT+TGG - tig0024531:56113-56132 None:intergenic 30.0%
TAGTCAGTCTATAAGAAAGA+AGG - tig0024531:56165-56184 None:intergenic 30.0%
ATATTGATCATGACGATGAT+GGG + tig0024531:56215-56234 Msa1417950:exon 30.0%
TGTTCTCTTAATGTGTCTTT+CGG - tig0024531:56332-56351 None:intergenic 30.0%
AGCTGTCATAATTTCTGATA+AGG + tig0024531:56379-56398 Msa1417950:CDS 30.0%
!! ATGATGATGATGGAATATTG+GGG - tig0024531:56645-56664 None:intergenic 30.0%
!! GATGATGATGATGGAATATT+GGG - tig0024531:56646-56665 None:intergenic 30.0%
!! TGATGATGATGATGGAATAT+TGG - tig0024531:56647-56666 None:intergenic 30.0%
! AAATCACATTTGTTAAGGTC+GGG - tig0024531:56809-56828 None:intergenic 30.0%
TATACCTGAATGAAAGAAAG+AGG - tig0024531:56842-56861 None:intergenic 30.0%
TTACAACTGCAATTACAATC+AGG + tig0024531:56893-56912 Msa1417950:CDS 30.0%
GGCATGCAATGTAATAATAT+TGG - tig0024531:56934-56953 None:intergenic 30.0%
GATATTGATCATGACGATGA+TGG + tig0024531:56214-56233 Msa1417950:exon 35.0%
CAGAAATTATGACAGCTTCA+AGG - tig0024531:56376-56395 None:intergenic 35.0%
TGTTACAAATCCATACCCTT+TGG - tig0024531:56417-56436 None:intergenic 35.0%
TGTAACATTCAAAGATGCAG+AGG + tig0024531:56430-56449 Msa1417950:CDS 35.0%
TATATACCCGTAGAAAGGAA+GGG - tig0024531:56675-56694 None:intergenic 35.0%
ATATATACCCGTAGAAAGGA+AGG - tig0024531:56676-56695 None:intergenic 35.0%
!!! CTTGTTTGTTTTGGGTGAAA+GGG + tig0024531:56777-56796 Msa1417950:intron 35.0%
ATCTCCTCTTTCTTTCATTC+AGG + tig0024531:56835-56854 Msa1417950:intron 35.0%
CATATGATCTGTTGCAATGT+AGG - tig0024531:56871-56890 None:intergenic 35.0%
ACTGCAATTACAATCAGGTA+TGG + tig0024531:56898-56917 Msa1417950:intron 35.0%
! AATGATAATGATGAGTTGGG+TGG - tig0024531:57165-57184 None:intergenic 35.0%
AAAGTGACAGTTTGTGTTTG+TGG + tig0024531:57290-57309 Msa1417950:intron 35.0%
AAGTGACAGTTTGTGTTTGT+GGG + tig0024531:57291-57310 Msa1417950:intron 35.0%
!! TGTGGGTGTTGAATATTGAT+AGG + tig0024531:57308-57327 Msa1417950:intron 35.0%
TGAATATTGATAGGTCCAGT+TGG + tig0024531:57317-57336 Msa1417950:intron 35.0%
GATATTGATCATGACGATGA+TGG + tig0024531:56214-56233 Msa1417950:exon 35.0%
CAGAAATTATGACAGCTTCA+AGG - tig0024531:56376-56395 None:intergenic 35.0%
TGTTACAAATCCATACCCTT+TGG - tig0024531:56417-56436 None:intergenic 35.0%
TGTAACATTCAAAGATGCAG+AGG + tig0024531:56430-56449 Msa1417950:CDS 35.0%
TATATACCCGTAGAAAGGAA+GGG - tig0024531:56675-56694 None:intergenic 35.0%
ATATATACCCGTAGAAAGGA+AGG - tig0024531:56676-56695 None:intergenic 35.0%
!!! CTTGTTTGTTTTGGGTGAAA+GGG + tig0024531:56777-56796 Msa1417950:intron 35.0%
ATCTCCTCTTTCTTTCATTC+AGG + tig0024531:56835-56854 Msa1417950:intron 35.0%
CATATGATCTGTTGCAATGT+AGG - tig0024531:56871-56890 None:intergenic 35.0%
ACTGCAATTACAATCAGGTA+TGG + tig0024531:56898-56917 Msa1417950:intron 35.0%
! AATGATAATGATGAGTTGGG+TGG - tig0024531:57165-57184 None:intergenic 35.0%
AAAGTGACAGTTTGTGTTTG+TGG + tig0024531:57290-57309 Msa1417950:intron 35.0%
AAGTGACAGTTTGTGTTTGT+GGG + tig0024531:57291-57310 Msa1417950:intron 35.0%
!! TGTGGGTGTTGAATATTGAT+AGG + tig0024531:57308-57327 Msa1417950:intron 35.0%
TGAATATTGATAGGTCCAGT+TGG + tig0024531:57317-57336 Msa1417950:intron 35.0%
AATGGATCACCATCACAGTT+TGG + tig0024531:56257-56276 Msa1417950:CDS 40.0%
ATGGATCACCATCACAGTTT+GGG + tig0024531:56258-56277 Msa1417950:CDS 40.0%
!! ACGCTGACAAAAGTGTTTGT+TGG + tig0024531:56287-56306 Msa1417950:CDS 40.0%
!! CTGACAAAAGTGTTTGTTGG+AGG + tig0024531:56290-56309 Msa1417950:CDS 40.0%
GAACCAACTGTGATCATCAA+TGG + tig0024531:56473-56492 Msa1417950:CDS 40.0%
AAGAGAAGCGATATTGCAGT+TGG - tig0024531:56507-56526 None:intergenic 40.0%
AACTGCAATATCGCTTCTCT+TGG + tig0024531:56506-56525 Msa1417950:CDS 40.0%
GCAGGTTCAATGGTATTATC+CGG + tig0024531:56598-56617 Msa1417950:CDS 40.0%
GTTCAATGGTATTATCCGGT+GGG + tig0024531:56602-56621 Msa1417950:CDS 40.0%
!! TGATGATGGAATATTGGGGA+AGG - tig0024531:56641-56660 None:intergenic 40.0%
AGAGAGAGAGAGAGAGATAT+GGG + tig0024531:56749-56768 Msa1417950:intron 40.0%
!! TGGGACATCCTTGTTTGTTT+TGG + tig0024531:56768-56787 Msa1417950:intron 40.0%
!! GGGACATCCTTGTTTGTTTT+GGG + tig0024531:56769-56788 Msa1417950:intron 40.0%
CCTTTCACCCAAAACAAACA+AGG - tig0024531:56779-56798 None:intergenic 40.0%
!!! CCTTGTTTGTTTTGGGTGAA+AGG + tig0024531:56776-56795 Msa1417950:intron 40.0%
TGATCTGTTGCAATGTAGGT+GGG - tig0024531:56867-56886 None:intergenic 40.0%
ATGATCTGTTGCAATGTAGG+TGG - tig0024531:56868-56887 None:intergenic 40.0%
CAGAAACTGAGCTATGGAAT+GGG + tig0024531:57010-57029 Msa1417950:intron 40.0%
TAACCTGTTGTAGTAGGACT+AGG - tig0024531:57205-57224 None:intergenic 40.0%
TGAATGCTTCAAAAGGGTAG+TGG + tig0024531:57346-57365 Msa1417950:CDS 40.0%
AATGGATCACCATCACAGTT+TGG + tig0024531:56257-56276 Msa1417950:CDS 40.0%
ATGGATCACCATCACAGTTT+GGG + tig0024531:56258-56277 Msa1417950:CDS 40.0%
!! ACGCTGACAAAAGTGTTTGT+TGG + tig0024531:56287-56306 Msa1417950:CDS 40.0%
!! CTGACAAAAGTGTTTGTTGG+AGG + tig0024531:56290-56309 Msa1417950:CDS 40.0%
GAACCAACTGTGATCATCAA+TGG + tig0024531:56473-56492 Msa1417950:CDS 40.0%
AAGAGAAGCGATATTGCAGT+TGG - tig0024531:56507-56526 None:intergenic 40.0%
AACTGCAATATCGCTTCTCT+TGG + tig0024531:56506-56525 Msa1417950:CDS 40.0%
GCAGGTTCAATGGTATTATC+CGG + tig0024531:56598-56617 Msa1417950:CDS 40.0%
GTTCAATGGTATTATCCGGT+GGG + tig0024531:56602-56621 Msa1417950:CDS 40.0%
!! TGATGATGGAATATTGGGGA+AGG - tig0024531:56641-56660 None:intergenic 40.0%
AGAGAGAGAGAGAGAGATAT+GGG + tig0024531:56749-56768 Msa1417950:intron 40.0%
!! TGGGACATCCTTGTTTGTTT+TGG + tig0024531:56768-56787 Msa1417950:intron 40.0%
!! GGGACATCCTTGTTTGTTTT+GGG + tig0024531:56769-56788 Msa1417950:intron 40.0%
CCTTTCACCCAAAACAAACA+AGG - tig0024531:56779-56798 None:intergenic 40.0%
!!! CCTTGTTTGTTTTGGGTGAA+AGG + tig0024531:56776-56795 Msa1417950:intron 40.0%
TGATCTGTTGCAATGTAGGT+GGG - tig0024531:56867-56886 None:intergenic 40.0%
ATGATCTGTTGCAATGTAGG+TGG - tig0024531:56868-56887 None:intergenic 40.0%
CAGAAACTGAGCTATGGAAT+GGG + tig0024531:57010-57029 Msa1417950:intron 40.0%
TAACCTGTTGTAGTAGGACT+AGG - tig0024531:57205-57224 None:intergenic 40.0%
TGAATGCTTCAAAAGGGTAG+TGG + tig0024531:57346-57365 Msa1417950:CDS 40.0%
AGCAGCAACAGCAACAACAA+TGG + tig0024531:56239-56258 Msa1417950:CDS 45.0%
TGGATCACCATCACAGTTTG+GGG + tig0024531:56259-56278 Msa1417950:CDS 45.0%
! CGACCATTGATGATCACAGT+TGG - tig0024531:56479-56498 None:intergenic 45.0%
AAGCGATATTGCAGTTGGCT+CGG - tig0024531:56502-56521 None:intergenic 45.0%
TTATGGGAGAAGACCTTGGA+CGG - tig0024531:56538-56557 None:intergenic 45.0%
GAAGTTATGGGAGAAGACCT+TGG - tig0024531:56542-56561 None:intergenic 45.0%
AATACCATTGAACCTGCTGC+TGG - tig0024531:56595-56614 None:intergenic 45.0%
GGTTCAATGGTATTATCCGG+TGG + tig0024531:56601-56620 Msa1417950:CDS 45.0%
! AATATTGGGGAAGGAGGTGT+GGG - tig0024531:56632-56651 None:intergenic 45.0%
TGATGGAATATTGGGGAAGG+AGG - tig0024531:56638-56657 None:intergenic 45.0%
TATCTCTCTCTCTCTCTCTC+TGG - tig0024531:56748-56767 None:intergenic 45.0%
GAGAGAGAGAGAGAGAGATA+TGG + tig0024531:56748-56767 Msa1417950:intron 45.0%
CGAATGCAGAAACTGAGCTA+TGG + tig0024531:57004-57023 Msa1417950:intron 45.0%
GCAGAAACTGAGCTATGGAA+TGG + tig0024531:57009-57028 Msa1417950:intron 45.0%
AAACTGAGCTATGGAATGGG+TGG + tig0024531:57013-57032 Msa1417950:CDS 45.0%
AACTGAGCTATGGAATGGGT+GGG + tig0024531:57014-57033 Msa1417950:CDS 45.0%
! TGAGTTGGGTGGAAGATTTG+AGG - tig0024531:57154-57173 None:intergenic 45.0%
!! TAGGACTAGGAGTGATGGAT+GGG - tig0024531:57192-57211 None:intergenic 45.0%
TGTAGTAGGACTAGGAGTGA+TGG - tig0024531:57197-57216 None:intergenic 45.0%
ACTCCTAGTCCTACTACAAC+AGG + tig0024531:57199-57218 Msa1417950:CDS 45.0%
ATTGATAGGTCCAGTTGGCA+CGG + tig0024531:57322-57341 Msa1417950:intron 45.0%
CACGGGTGAATGCTTCAAAA+GGG + tig0024531:57340-57359 Msa1417950:CDS 45.0%
AGCAGCAACAGCAACAACAA+TGG + tig0024531:56239-56258 Msa1417950:CDS 45.0%
TGGATCACCATCACAGTTTG+GGG + tig0024531:56259-56278 Msa1417950:CDS 45.0%
! CGACCATTGATGATCACAGT+TGG - tig0024531:56479-56498 None:intergenic 45.0%
AAGCGATATTGCAGTTGGCT+CGG - tig0024531:56502-56521 None:intergenic 45.0%
TTATGGGAGAAGACCTTGGA+CGG - tig0024531:56538-56557 None:intergenic 45.0%
GAAGTTATGGGAGAAGACCT+TGG - tig0024531:56542-56561 None:intergenic 45.0%
AATACCATTGAACCTGCTGC+TGG - tig0024531:56595-56614 None:intergenic 45.0%
GGTTCAATGGTATTATCCGG+TGG + tig0024531:56601-56620 Msa1417950:CDS 45.0%
! AATATTGGGGAAGGAGGTGT+GGG - tig0024531:56632-56651 None:intergenic 45.0%
TGATGGAATATTGGGGAAGG+AGG - tig0024531:56638-56657 None:intergenic 45.0%
TATCTCTCTCTCTCTCTCTC+TGG - tig0024531:56748-56767 None:intergenic 45.0%
GAGAGAGAGAGAGAGAGATA+TGG + tig0024531:56748-56767 Msa1417950:intron 45.0%
CGAATGCAGAAACTGAGCTA+TGG + tig0024531:57004-57023 Msa1417950:intron 45.0%
GCAGAAACTGAGCTATGGAA+TGG + tig0024531:57009-57028 Msa1417950:intron 45.0%
AAACTGAGCTATGGAATGGG+TGG + tig0024531:57013-57032 Msa1417950:CDS 45.0%
AACTGAGCTATGGAATGGGT+GGG + tig0024531:57014-57033 Msa1417950:CDS 45.0%
! TGAGTTGGGTGGAAGATTTG+AGG - tig0024531:57154-57173 None:intergenic 45.0%
!! TAGGACTAGGAGTGATGGAT+GGG - tig0024531:57192-57211 None:intergenic 45.0%
TGTAGTAGGACTAGGAGTGA+TGG - tig0024531:57197-57216 None:intergenic 45.0%
ACTCCTAGTCCTACTACAAC+AGG + tig0024531:57199-57218 Msa1417950:CDS 45.0%
ATTGATAGGTCCAGTTGGCA+CGG + tig0024531:57322-57341 Msa1417950:intron 45.0%
CACGGGTGAATGCTTCAAAA+GGG + tig0024531:57340-57359 Msa1417950:CDS 45.0%
!!! TTAATTTTAATTTGAAAAAA+TGG + tig0024531:56976-56995 Msa1417950:intron 5.0%
!!! AGTTTAATTTATAAATAAAA+GGG - tig0024531:57238-57257 None:intergenic 5.0%
!!! AAGTTTAATTTATAAATAAA+AGG - tig0024531:57239-57258 None:intergenic 5.0%
!!! TTAATTTTAATTTGAAAAAA+TGG + tig0024531:56976-56995 Msa1417950:intron 5.0%
!!! AGTTTAATTTATAAATAAAA+GGG - tig0024531:57238-57257 None:intergenic 5.0%
!!! AAGTTTAATTTATAAATAAA+AGG - tig0024531:57239-57258 None:intergenic 5.0%
GTTGTGTCCCCAAACTGTGA+TGG - tig0024531:56269-56288 None:intergenic 50.0%
! TGTTTGTTGGAGGTCTTGCC+TGG + tig0024531:56300-56319 Msa1417950:CDS 50.0%
! GTTTGTTGGAGGTCTTGCCT+GGG + tig0024531:56301-56320 Msa1417950:CDS 50.0%
TGTGTCTTTCGGAGTTTCCC+AGG - tig0024531:56321-56340 None:intergenic 50.0%
AAGGTTACCGGCAGATCCAA+AGG + tig0024531:56398-56417 Msa1417950:CDS 50.0%
AGGTTACCGGCAGATCCAAA+GGG + tig0024531:56399-56418 Msa1417950:CDS 50.0%
TCCATACCCTTTGGATCTGC+CGG - tig0024531:56408-56427 None:intergenic 50.0%
AGATGCAGAGGCTGCAAAGA+AGG + tig0024531:56442-56461 Msa1417950:CDS 50.0%
GAATATTGGGGAAGGAGGTG+TGG - tig0024531:56633-56652 None:intergenic 50.0%
! GGGCGTGATGATGATGATGA+TGG - tig0024531:56655-56674 None:intergenic 50.0%
CATCACGCCCTTCCTTTCTA+CGG + tig0024531:56665-56684 Msa1417950:CDS 50.0%
ATCACGCCCTTCCTTTCTAC+GGG + tig0024531:56666-56685 Msa1417950:CDS 50.0%
ACTGAGCTATGGAATGGGTG+GGG + tig0024531:57015-57034 Msa1417950:CDS 50.0%
ATCTGTAGACAGGAGGAGCA+GGG - tig0024531:57099-57118 None:intergenic 50.0%
TCGGTTGATCTGTAGACAGG+AGG - tig0024531:57106-57125 None:intergenic 50.0%
TCCTGTCTACAGATCAACCG+AGG + tig0024531:57105-57124 Msa1417950:CDS 50.0%
CTACAGATCAACCGAGGCTA+TGG + tig0024531:57111-57130 Msa1417950:CDS 50.0%
! GGTGGAAGATTTGAGGTGGA+GGG - tig0024531:57147-57166 None:intergenic 50.0%
GTTGGGTGGAAGATTTGAGG+TGG - tig0024531:57151-57170 None:intergenic 50.0%
!! AGGACTAGGAGTGATGGATG+GGG - tig0024531:57191-57210 None:intergenic 50.0%
! GTAGGACTAGGAGTGATGGA+TGG - tig0024531:57193-57212 None:intergenic 50.0%
TTGATAGGTCCAGTTGGCAC+GGG + tig0024531:57323-57342 Msa1417950:intron 50.0%
GCACGGGTGAATGCTTCAAA+AGG + tig0024531:57339-57358 Msa1417950:CDS 50.0%
GTTGTGTCCCCAAACTGTGA+TGG - tig0024531:56269-56288 None:intergenic 50.0%
! TGTTTGTTGGAGGTCTTGCC+TGG + tig0024531:56300-56319 Msa1417950:CDS 50.0%
! GTTTGTTGGAGGTCTTGCCT+GGG + tig0024531:56301-56320 Msa1417950:CDS 50.0%
TGTGTCTTTCGGAGTTTCCC+AGG - tig0024531:56321-56340 None:intergenic 50.0%
AAGGTTACCGGCAGATCCAA+AGG + tig0024531:56398-56417 Msa1417950:CDS 50.0%
AGGTTACCGGCAGATCCAAA+GGG + tig0024531:56399-56418 Msa1417950:CDS 50.0%
TCCATACCCTTTGGATCTGC+CGG - tig0024531:56408-56427 None:intergenic 50.0%
AGATGCAGAGGCTGCAAAGA+AGG + tig0024531:56442-56461 Msa1417950:CDS 50.0%
GAATATTGGGGAAGGAGGTG+TGG - tig0024531:56633-56652 None:intergenic 50.0%
! GGGCGTGATGATGATGATGA+TGG - tig0024531:56655-56674 None:intergenic 50.0%
CATCACGCCCTTCCTTTCTA+CGG + tig0024531:56665-56684 Msa1417950:CDS 50.0%
ATCACGCCCTTCCTTTCTAC+GGG + tig0024531:56666-56685 Msa1417950:CDS 50.0%
ACTGAGCTATGGAATGGGTG+GGG + tig0024531:57015-57034 Msa1417950:CDS 50.0%
ATCTGTAGACAGGAGGAGCA+GGG - tig0024531:57099-57118 None:intergenic 50.0%
TCGGTTGATCTGTAGACAGG+AGG - tig0024531:57106-57125 None:intergenic 50.0%
TCCTGTCTACAGATCAACCG+AGG + tig0024531:57105-57124 Msa1417950:CDS 50.0%
CTACAGATCAACCGAGGCTA+TGG + tig0024531:57111-57130 Msa1417950:CDS 50.0%
! GGTGGAAGATTTGAGGTGGA+GGG - tig0024531:57147-57166 None:intergenic 50.0%
GTTGGGTGGAAGATTTGAGG+TGG - tig0024531:57151-57170 None:intergenic 50.0%
!! AGGACTAGGAGTGATGGATG+GGG - tig0024531:57191-57210 None:intergenic 50.0%
! GTAGGACTAGGAGTGATGGA+TGG - tig0024531:57193-57212 None:intergenic 50.0%
TTGATAGGTCCAGTTGGCAC+GGG + tig0024531:57323-57342 Msa1417950:intron 50.0%
GCACGGGTGAATGCTTCAAA+AGG + tig0024531:57339-57358 Msa1417950:CDS 50.0%
ACCGGCAGATCCAAAGGGTA+TGG + tig0024531:56404-56423 Msa1417950:CDS 55.0%
GGAGGAGGAGGCGAAGTTAT+GGG - tig0024531:56554-56573 None:intergenic 55.0%
TGGAGGAGGAGGCGAAGTTA+TGG - tig0024531:56555-56574 None:intergenic 55.0%
TTGCTGCGGTGGATCTTTGG+AGG - tig0024531:56572-56591 None:intergenic 55.0%
TGGTTGCTGCGGTGGATCTT+TGG - tig0024531:56575-56594 None:intergenic 55.0%
GCAACCAGCAGCAGGTTCAA+TGG + tig0024531:56588-56607 Msa1417950:CDS 55.0%
CTGAGCTATGGAATGGGTGG+GGG + tig0024531:57016-57035 Msa1417950:CDS 55.0%
! GCAGGGTGGTGGTACATGAT+TGG - tig0024531:57082-57101 None:intergenic 55.0%
GATCTGTAGACAGGAGGAGC+AGG - tig0024531:57100-57119 None:intergenic 55.0%
GCCTCGGTTGATCTGTAGAC+AGG - tig0024531:57109-57128 None:intergenic 55.0%
ATCAACCGAGGCTATGGCCA+TGG + tig0024531:57117-57136 Msa1417950:CDS 55.0%
TCAACCGAGGCTATGGCCAT+GGG + tig0024531:57118-57137 Msa1417950:CDS 55.0%
! GGGTGGAAGATTTGAGGTGG+AGG - tig0024531:57148-57167 None:intergenic 55.0%
AAGCATTCACCCGTGCCAAC+TGG - tig0024531:57335-57354 None:intergenic 55.0%
ACCGGCAGATCCAAAGGGTA+TGG + tig0024531:56404-56423 Msa1417950:CDS 55.0%
GGAGGAGGAGGCGAAGTTAT+GGG - tig0024531:56554-56573 None:intergenic 55.0%
TGGAGGAGGAGGCGAAGTTA+TGG - tig0024531:56555-56574 None:intergenic 55.0%
TTGCTGCGGTGGATCTTTGG+AGG - tig0024531:56572-56591 None:intergenic 55.0%
TGGTTGCTGCGGTGGATCTT+TGG - tig0024531:56575-56594 None:intergenic 55.0%
GCAACCAGCAGCAGGTTCAA+TGG + tig0024531:56588-56607 Msa1417950:CDS 55.0%
CTGAGCTATGGAATGGGTGG+GGG + tig0024531:57016-57035 Msa1417950:CDS 55.0%
! GCAGGGTGGTGGTACATGAT+TGG - tig0024531:57082-57101 None:intergenic 55.0%
GATCTGTAGACAGGAGGAGC+AGG - tig0024531:57100-57119 None:intergenic 55.0%
GCCTCGGTTGATCTGTAGAC+AGG - tig0024531:57109-57128 None:intergenic 55.0%
ATCAACCGAGGCTATGGCCA+TGG + tig0024531:57117-57136 Msa1417950:CDS 55.0%
TCAACCGAGGCTATGGCCAT+GGG + tig0024531:57118-57137 Msa1417950:CDS 55.0%
! GGGTGGAAGATTTGAGGTGG+AGG - tig0024531:57148-57167 None:intergenic 55.0%
AAGCATTCACCCGTGCCAAC+TGG - tig0024531:57335-57354 None:intergenic 55.0%
GGCTGCAAAGAAGGCTTGCG+AGG + tig0024531:56451-56470 Msa1417950:CDS 60.0%
! CGGTGGATCTTTGGAGGAGG+AGG - tig0024531:56566-56585 None:intergenic 60.0%
! CTGCGGTGGATCTTTGGAGG+AGG - tig0024531:56569-56588 None:intergenic 60.0%
! GAACCTGCTGCTGGTTGCTG+CGG - tig0024531:56586-56605 None:intergenic 60.0%
GCACCCACAATAGCCTGCCT+CGG - tig0024531:57055-57074 None:intergenic 60.0%
TGTAGACAGGAGGAGCAGGG+TGG - tig0024531:57096-57115 None:intergenic 60.0%
GACACCCATGGCCATAGCCT+CGG - tig0024531:57125-57144 None:intergenic 60.0%
TTGAGGTGGAGGGACACCCA+TGG - tig0024531:57137-57156 None:intergenic 60.0%
GGCTGCAAAGAAGGCTTGCG+AGG + tig0024531:56451-56470 Msa1417950:CDS 60.0%
! CGGTGGATCTTTGGAGGAGG+AGG - tig0024531:56566-56585 None:intergenic 60.0%
! CTGCGGTGGATCTTTGGAGG+AGG - tig0024531:56569-56588 None:intergenic 60.0%
! GAACCTGCTGCTGGTTGCTG+CGG - tig0024531:56586-56605 None:intergenic 60.0%
GCACCCACAATAGCCTGCCT+CGG - tig0024531:57055-57074 None:intergenic 60.0%
TGTAGACAGGAGGAGCAGGG+TGG - tig0024531:57096-57115 None:intergenic 60.0%
GACACCCATGGCCATAGCCT+CGG - tig0024531:57125-57144 None:intergenic 60.0%
TTGAGGTGGAGGGACACCCA+TGG - tig0024531:57137-57156 None:intergenic 60.0%
!! CTCTTGGTGCTCGCCGTCCA+AGG + tig0024531:56522-56541 Msa1417950:CDS 65.0%
! GGAGGTGTGGGTGTTCCCAC+CGG - tig0024531:56620-56639 None:intergenic 65.0%
CCACAATAGCCTGCCTCGGC+GGG - tig0024531:57051-57070 None:intergenic 65.0%
CCCACAATAGCCTGCCTCGG+CGG - tig0024531:57052-57071 None:intergenic 65.0%
CCGCCGAGGCAGGCTATTGT+GGG + tig0024531:57049-57068 Msa1417950:CDS 65.0%
AGACAGGAGGAGCAGGGTGG+TGG - tig0024531:57093-57112 None:intergenic 65.0%
!! CTCTTGGTGCTCGCCGTCCA+AGG + tig0024531:56522-56541 Msa1417950:CDS 65.0%
! GGAGGTGTGGGTGTTCCCAC+CGG - tig0024531:56620-56639 None:intergenic 65.0%
CCACAATAGCCTGCCTCGGC+GGG - tig0024531:57051-57070 None:intergenic 65.0%
CCCACAATAGCCTGCCTCGG+CGG - tig0024531:57052-57071 None:intergenic 65.0%
CCGCCGAGGCAGGCTATTGT+GGG + tig0024531:57049-57068 Msa1417950:CDS 65.0%
AGACAGGAGGAGCAGGGTGG+TGG - tig0024531:57093-57112 None:intergenic 65.0%
! CCTGCTGCTGGTTGCTGCGG+TGG - tig0024531:56583-56602 None:intergenic 70.0%
CCACCGCAGCAACCAGCAGC+AGG + tig0024531:56580-56599 Msa1417950:CDS 70.0%
CTACGTGTACCCGCCGAGGC+AGG + tig0024531:57039-57058 Msa1417950:CDS 70.0%
CCCGCCGAGGCAGGCTATTG+TGG + tig0024531:57048-57067 Msa1417950:CDS 70.0%
! CCTGCTGCTGGTTGCTGCGG+TGG - tig0024531:56583-56602 None:intergenic 70.0%
CCACCGCAGCAACCAGCAGC+AGG + tig0024531:56580-56599 Msa1417950:CDS 70.0%
CTACGTGTACCCGCCGAGGC+AGG + tig0024531:57039-57058 Msa1417950:CDS 70.0%
CCCGCCGAGGCAGGCTATTG+TGG + tig0024531:57048-57067 Msa1417950:CDS 70.0%
GGGGCTACGTGTACCCGCCG+AGG + tig0024531:57035-57054 Msa1417950:CDS 75.0%
GGGGCTACGTGTACCCGCCG+AGG + tig0024531:57035-57054 Msa1417950:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0024531 gene 56110 57373 56110 ID=Msa1417950;Name=Msa1417950
tig0024531 mRNA 56110 57373 56110 ID=Msa1417950-mRNA-1;Parent=Msa1417950;Name=Msa1417950-mRNA-1;_AED=0.02;_eAED=0.02;_QI=114|0.66|0.5|1|1|1|4|0|257
tig0024531 exon 56110 56687 56110 ID=Msa1417950-mRNA-1:exon:117;Parent=Msa1417950-mRNA-1
tig0024531 exon 56857 56914 56857 ID=Msa1417950-mRNA-1:exon:118;Parent=Msa1417950-mRNA-1
tig0024531 exon 57013 57220 57013 ID=Msa1417950-mRNA-1:exon:119;Parent=Msa1417950-mRNA-1
tig0024531 exon 57330 57373 57330 ID=Msa1417950-mRNA-1:exon:120;Parent=Msa1417950-mRNA-1
tig0024531 five_prime_UTR 56110 56223 56110 ID=Msa1417950-mRNA-1:five_prime_utr;Parent=Msa1417950-mRNA-1
tig0024531 CDS 56224 56687 56224 ID=Msa1417950-mRNA-1:cds;Parent=Msa1417950-mRNA-1
tig0024531 CDS 56857 56914 56857 ID=Msa1417950-mRNA-1:cds;Parent=Msa1417950-mRNA-1
tig0024531 CDS 57013 57220 57013 ID=Msa1417950-mRNA-1:cds;Parent=Msa1417950-mRNA-1
tig0024531 CDS 57330 57373 57330 ID=Msa1417950-mRNA-1:cds;Parent=Msa1417950-mRNA-1
Gene Sequence

>Msa1417950

ATGACGATGATGGGCAGCAGCAACAGCAACAACAATGGATCACCATCACAGTTTGGGGACACAACGCTGACAAAAGTGTTTGTTGGAGGTCTTGCCTGGGAAACTCCGAAAGACACATTAAGAGAACACTTTGAAAAATACGGAGACATCCTTGAAGCTGTCATAATTTCTGATAAGGTTACCGGCAGATCCAAAGGGTATGGATTTGTAACATTCAAAGATGCAGAGGCTGCAAAGAAGGCTTGCGAGGAACCAACTGTGATCATCAATGGTCGCCGAGCCAACTGCAATATCGCTTCTCTTGGTGCTCGCCGTCCAAGGTCTTCTCCCATAACTTCGCCTCCTCCTCCAAAGATCCACCGCAGCAACCAGCAGCAGGTTCAATGGTATTATCCGGTGGGAACACCCACACCTCCTTCCCCAATATTCCATCATCATCATCATCACGCCCTTCCTTTCTACGGGTATACGCCCACCTACATTGCAACAGATCATATGAATTACAACTGCAATTACAATCAGAAACTGAGCTATGGAATGGGTGGGGGCTACGTGTACCCGCCGAGGCAGGCTATTGTGGGTGCGATGCCAATCATGTACCACCACCCTGCTCCTCCTGTCTACAGATCAACCGAGGCTATGGCCATGGGTGTCCCTCCACCTCAAATCTTCCACCCAACTCATCATTATCATTATTCCCCATCCATCACTCCTAGTCCTACTACAACAGGTCCAGTTGGCACGGGTGAATGCTTCAAAAGGGTAGTGGTCTGA
Protein sequence

>Msa1417950

MTMMGSSNSNNNGSPSQFGDTTLTKVFVGGLAWETPKDTLREHFEKYGDILEAVIISDKVTGRSKGYGFVTFKDAEAAKKACEEPTVIINGRRANCNIASLGARRPRSSPITSPPPPKIHRSNQQQVQWYYPVGTPTPPSPIFHHHHHHALPFYGYTPTYIATDHMNYNCNYNQKLSYGMGGGYVYPPRQAIVGAMPIMYHHPAPPVYRSTEAMAMGVPPPQIFHPTHHYHYSPSITPSPTTTGPVGTGECFKRVVV*