Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1314190 | PNX78576.1 | 91.566 | 83 | 4 | 1 | 1 | 80 | 1 | 83 | 9.61e-42 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1314190 | sp|P24095|LOXX_SOYBN | 80.519 | 77 | 15 | 0 | 1 | 77 | 1 | 77 | 1.67e-36 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1314190 | A0A2K3LVY8 | 91.566 | 83 | 4 | 1 | 1 | 80 | 1 | 83 | 4.59e-42 | 141 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0626460 | Msa1314190 | 0.823133 | 1.632269e-53 | -8.615850e-47 |
| Msa0664540 | Msa1314190 | 0.805982 | 1.025950e-49 | -8.615850e-47 |
| Msa0226310 | Msa1314190 | 0.838736 | 2.409191e-57 | -8.615850e-47 |
| Msa1233670 | Msa1314190 | 0.831116 | 2.002559e-55 | -8.615850e-47 |
| Msa1267040 | Msa1314190 | 0.811431 | 7.026997e-51 | -8.615850e-47 |
| Msa1267170 | Msa1314190 | 0.809934 | 1.480560e-50 | -8.615850e-47 |
| Msa1275160 | Msa1314190 | 0.817403 | 3.357595e-52 | -8.615850e-47 |
| Msa1275930 | Msa1314190 | 0.812486 | 4.138755e-51 | -8.615850e-47 |
| Msa1314080 | Msa1314190 | 0.836908 | 7.103739e-57 | -8.615850e-47 |
| Msa1314190 | Msa1341840 | 0.813770 | 2.163062e-51 | -8.615850e-47 |
| Msa1314190 | Msa1382710 | 0.818397 | 2.002093e-52 | -8.615850e-47 |
| Msa1314190 | Msa1403710 | 0.800836 | 1.195421e-48 | -8.615850e-47 |
| Msa1314190 | Msa1440900 | 0.801232 | 9.923491e-49 | -8.615850e-47 |
| Msa1314190 | Msa1461370 | 0.829919 | 3.932615e-55 | -8.615850e-47 |
| Msa0427700 | Msa1314190 | 0.825371 | 4.863113e-54 | -8.615850e-47 |
| Msa0984670 | Msa1314190 | 0.802322 | 5.928069e-49 | -8.615850e-47 |
| Msa0304570 | Msa1314190 | 0.840478 | 8.488940e-58 | -8.615850e-47 |
| Msa0304680 | Msa1314190 | 0.843981 | 1.002629e-58 | -8.615850e-47 |
| Msa0586450 | Msa1314190 | 0.823968 | 1.040932e-53 | -8.615850e-47 |
| Msa0883040 | Msa1314190 | 0.835670 | 1.466174e-56 | -8.615850e-47 |
| Msa0910410 | Msa1314190 | 0.852041 | 5.987998e-61 | -8.615850e-47 |
| Msa0948270 | Msa1314190 | 0.802441 | 5.601219e-49 | -8.615850e-47 |
| Msa0948280 | Msa1314190 | 0.802945 | 4.409618e-49 | -8.615850e-47 |
| Msa0979180 | Msa1314190 | 0.801593 | 8.367884e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1314190 | MtrunA17_Chr8g0343021 | 98.701 | 77 | 1 | 0 | 1 | 77 | 1 | 77 | 1.19e-45 | 155 |
| Msa1314190 | MtrunA17_Chr5g0406871 | 73.418 | 79 | 19 | 1 | 1 | 77 | 1 | 79 | 1.22e-33 | 121 |
| Msa1314190 | MtrunA17_Chr8g0343031 | 87.179 | 78 | 9 | 1 | 1 | 77 | 1 | 78 | 5.16e-30 | 111 |
| Msa1314190 | MtrunA17_Chr2g0329901 | 62.821 | 78 | 19 | 2 | 3 | 77 | 7 | 77 | 4.48e-22 | 88.6 |
| Msa1314190 | MtrunA17_Chr8g0342951 | 51.316 | 76 | 33 | 2 | 3 | 77 | 7 | 79 | 4.23e-19 | 80.1 |
| Msa1314190 | MtrunA17_Chr2g0329911 | 56.000 | 75 | 24 | 1 | 3 | 77 | 7 | 72 | 4.75e-19 | 79.7 |
| Msa1314190 | MtrunA17_Chr3g0120071 | 55.952 | 84 | 31 | 2 | 1 | 84 | 1 | 78 | 1.02e-18 | 79.0 |
| Msa1314190 | MtrunA17_Chr8g0343011 | 60.000 | 75 | 30 | 0 | 3 | 77 | 7 | 81 | 1.88e-16 | 72.4 |
| Msa1314190 | MtrunA17_Chr8g0342971 | 51.316 | 76 | 33 | 2 | 3 | 77 | 7 | 79 | 4.22e-16 | 71.6 |
| Msa1314190 | MtrunA17_Chr8g0344211 | 48.000 | 75 | 37 | 1 | 3 | 77 | 7 | 79 | 4.30e-16 | 71.6 |
| Msa1314190 | MtrunA17_Chr8g0342921 | 45.946 | 74 | 39 | 1 | 4 | 77 | 8 | 80 | 5.67e-14 | 65.5 |
| Msa1314190 | MtrunA17_Chr8g0342881 | 48.485 | 66 | 25 | 1 | 10 | 75 | 9 | 65 | 6.48e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTCCTCCGATCAAATTTC+CGG | 0.296832 | 8_4:-12735457 | None:intergenic |
| GATACTGCCACTGCCTTCTT+AGG | 0.334563 | 8_4:+12735504 | Msa1314190:CDS |
| GCCTTTACCGATGGACGTTA+TGG | 0.415758 | 8_4:-12735418 | None:intergenic |
| TCAACTGCATAGAGACATTA+CGG | 0.464666 | 8_4:-12735527 | None:intergenic |
| GGAGGAGTCACCAGCATTGT+TGG | 0.465982 | 8_4:+12735471 | Msa1314190:CDS |
| GGCGGTGTCCTCGACGCTGC+CGG | 0.489241 | 8_4:+12735438 | Msa1314190:CDS |
| AAAGAGTTAGCAAACATGTT+GGG | 0.505875 | 8_4:+12735324 | None:intergenic |
| GCCATAACGTCCATCGGTAA+AGG | 0.517311 | 8_4:+12735417 | Msa1314190:CDS |
| AACAACTCCACCAACAATGC+TGG | 0.526326 | 8_4:-12735481 | None:intergenic |
| ATGTTGGGCATCTTTGACAA+GGG | 0.541871 | 8_4:+12735339 | Msa1314190:CDS |
| AGAGACATTACGGCCTAAGA+AGG | 0.547564 | 8_4:-12735517 | None:intergenic |
| CATGTTGGGCATCTTTGACA+AGG | 0.564613 | 8_4:+12735338 | None:intergenic |
| GGAGTCACCAGCATTGTTGG+TGG | 0.578759 | 8_4:+12735474 | Msa1314190:CDS |
| ATTAGTGCTACAAAGACTGA+TGG | 0.579932 | 8_4:+12735549 | Msa1314190:CDS |
| GAGGACACCGCCTTTACCGA+TGG | 0.588131 | 8_4:-12735427 | None:intergenic |
| GACGCTGCCGGAAATTTGAT+CGG | 0.595337 | 8_4:+12735450 | Msa1314190:CDS |
| TCAAATTTCCGGCAGCGTCG+AGG | 0.595821 | 8_4:-12735446 | None:intergenic |
| TTCAACGCCATAACGTCCAT+CGG | 0.631507 | 8_4:+12735411 | Msa1314190:CDS |
| ATTACGGCCTAAGAAGGCAG+TGG | 0.637013 | 8_4:-12735511 | None:intergenic |
| GCTGCCGGAAATTTGATCGG+AGG | 0.683387 | 8_4:+12735453 | Msa1314190:CDS |
| ATAACGTCCATCGGTAAAGG+CGG | 0.695538 | 8_4:+12735420 | Msa1314190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAATTGAAGTATTATTTT+TGG | - | chr8_4:12735607-12735626 | None:intergenic | 10.0% |
| !!! | AATAATTGAAGTATTATTTT+TGG | - | chr8_4:12735607-12735626 | None:intergenic | 10.0% |
| !!! | TATTTAACTTTATGTGTTTT+TGG | + | chr8_4:12735676-12735695 | Msa1314190:intron | 15.0% |
| !!! | AGGAAGATAAATTATTTTAT+TGG | - | chr8_4:12735878-12735897 | None:intergenic | 15.0% |
| !! | TAAAGAGTTAAAATTATCAT+AGG | - | chr8_4:12735898-12735917 | None:intergenic | 15.0% |
| !! | GAAATAGTTAAAAAATATTG+AGG | + | chr8_4:12736070-12736089 | Msa1314190:intron | 15.0% |
| !! | TACTTATAAGATAAACTAAT+TGG | - | chr8_4:12736098-12736117 | None:intergenic | 15.0% |
| !!! | TATTTAACTTTATGTGTTTT+TGG | + | chr8_4:12735676-12735695 | Msa1314190:intron | 15.0% |
| !!! | AGGAAGATAAATTATTTTAT+TGG | - | chr8_4:12735878-12735897 | None:intergenic | 15.0% |
| !! | TAAAGAGTTAAAATTATCAT+AGG | - | chr8_4:12735898-12735917 | None:intergenic | 15.0% |
| !! | GAAATAGTTAAAAAATATTG+AGG | + | chr8_4:12736070-12736089 | Msa1314190:intron | 15.0% |
| !! | TACTTATAAGATAAACTAAT+TGG | - | chr8_4:12736098-12736117 | None:intergenic | 15.0% |
| !!! | GATAAATTATTTTATTGGCA+TGG | - | chr8_4:12735873-12735892 | None:intergenic | 20.0% |
| !!! | GATAAATTATTTTATTGGCA+TGG | - | chr8_4:12735873-12735892 | None:intergenic | 20.0% |
| !! | TTGAAGTCCAAAACATTTTT+GGG | - | chr8_4:12735396-12735415 | None:intergenic | 25.0% |
| ! | AGTTAAATATGTCTCTCTAT+AGG | - | chr8_4:12735665-12735684 | None:intergenic | 25.0% |
| ! | TAGCATATTGAATAAATGTG+TGG | - | chr8_4:12735707-12735726 | None:intergenic | 25.0% |
| !! | ATCGATAGTTAAGAGTTTTA+TGG | + | chr8_4:12735976-12735995 | Msa1314190:intron | 25.0% |
| !! | TTGAAGTCCAAAACATTTTT+GGG | - | chr8_4:12735396-12735415 | None:intergenic | 25.0% |
| ! | AGTTAAATATGTCTCTCTAT+AGG | - | chr8_4:12735665-12735684 | None:intergenic | 25.0% |
| ! | TAGCATATTGAATAAATGTG+TGG | - | chr8_4:12735707-12735726 | None:intergenic | 25.0% |
| !! | ATCGATAGTTAAGAGTTTTA+TGG | + | chr8_4:12735976-12735995 | Msa1314190:intron | 25.0% |
| ! | GTTGAAGTCCAAAACATTTT+TGG | - | chr8_4:12735397-12735416 | None:intergenic | 30.0% |
| ! | GTTGAAGTCCAAAACATTTT+TGG | - | chr8_4:12735397-12735416 | None:intergenic | 30.0% |
| ! | GTTGATGCCCAAAAATGTTT+TGG | + | chr8_4:12735386-12735405 | Msa1314190:CDS | 35.0% |
| TCAACTGCATAGAGACATTA+CGG | - | chr8_4:12735530-12735549 | None:intergenic | 35.0% | |
| ATTAGTGCTACAAAGACTGA+TGG | + | chr8_4:12735549-12735568 | Msa1314190:CDS | 35.0% | |
| ! | GTTGATGCCCAAAAATGTTT+TGG | + | chr8_4:12735386-12735405 | Msa1314190:CDS | 35.0% |
| TCAACTGCATAGAGACATTA+CGG | - | chr8_4:12735530-12735549 | None:intergenic | 35.0% | |
| ATTAGTGCTACAAAGACTGA+TGG | + | chr8_4:12735549-12735568 | Msa1314190:CDS | 35.0% | |
| ATGTTGGGCATCTTTGACAA+GGG | + | chr8_4:12735339-12735358 | Msa1314190:CDS | 40.0% | |
| TGACAAGGGCCAAAAAATCA+AGG | + | chr8_4:12735353-12735372 | Msa1314190:CDS | 40.0% | |
| GACAAGGGCCAAAAAATCAA+GGG | + | chr8_4:12735354-12735373 | Msa1314190:CDS | 40.0% | |
| ATGTTGGGCATCTTTGACAA+GGG | + | chr8_4:12735339-12735358 | Msa1314190:CDS | 40.0% | |
| TGACAAGGGCCAAAAAATCA+AGG | + | chr8_4:12735353-12735372 | Msa1314190:CDS | 40.0% | |
| GACAAGGGCCAAAAAATCAA+GGG | + | chr8_4:12735354-12735373 | Msa1314190:CDS | 40.0% | |
| !!! | CCACAGTGCCCTTGATTTTT+TGG | - | chr8_4:12735365-12735384 | None:intergenic | 45.0% |
| !! | CCAAAAAATCAAGGGCACTG+TGG | + | chr8_4:12735362-12735381 | Msa1314190:CDS | 45.0% |
| TTCAACGCCATAACGTCCAT+CGG | + | chr8_4:12735411-12735430 | Msa1314190:CDS | 45.0% | |
| ATAACGTCCATCGGTAAAGG+CGG | + | chr8_4:12735420-12735439 | Msa1314190:CDS | 45.0% | |
| GACTCCTCCGATCAAATTTC+CGG | - | chr8_4:12735460-12735479 | None:intergenic | 45.0% | |
| AACAACTCCACCAACAATGC+TGG | - | chr8_4:12735484-12735503 | None:intergenic | 45.0% | |
| AGAGACATTACGGCCTAAGA+AGG | - | chr8_4:12735520-12735539 | None:intergenic | 45.0% | |
| ! | TGAGCTTAGCTCAGTTGGTA+AGG | + | chr8_4:12735741-12735760 | Msa1314190:intron | 45.0% |
| !!! | CCACAGTGCCCTTGATTTTT+TGG | - | chr8_4:12735365-12735384 | None:intergenic | 45.0% |
| !! | CCAAAAAATCAAGGGCACTG+TGG | + | chr8_4:12735362-12735381 | Msa1314190:CDS | 45.0% |
| TTCAACGCCATAACGTCCAT+CGG | + | chr8_4:12735411-12735430 | Msa1314190:CDS | 45.0% | |
| ATAACGTCCATCGGTAAAGG+CGG | + | chr8_4:12735420-12735439 | Msa1314190:CDS | 45.0% | |
| GACTCCTCCGATCAAATTTC+CGG | - | chr8_4:12735460-12735479 | None:intergenic | 45.0% | |
| AACAACTCCACCAACAATGC+TGG | - | chr8_4:12735484-12735503 | None:intergenic | 45.0% | |
| AGAGACATTACGGCCTAAGA+AGG | - | chr8_4:12735520-12735539 | None:intergenic | 45.0% | |
| ! | TGAGCTTAGCTCAGTTGGTA+AGG | + | chr8_4:12735741-12735760 | Msa1314190:intron | 45.0% |
| GCCATAACGTCCATCGGTAA+AGG | + | chr8_4:12735417-12735436 | Msa1314190:CDS | 50.0% | |
| GCCTTTACCGATGGACGTTA+TGG | - | chr8_4:12735421-12735440 | None:intergenic | 50.0% | |
| GACGCTGCCGGAAATTTGAT+CGG | + | chr8_4:12735450-12735469 | Msa1314190:CDS | 50.0% | |
| GATACTGCCACTGCCTTCTT+AGG | + | chr8_4:12735504-12735523 | Msa1314190:CDS | 50.0% | |
| ATTACGGCCTAAGAAGGCAG+TGG | - | chr8_4:12735514-12735533 | None:intergenic | 50.0% | |
| GCCATAACGTCCATCGGTAA+AGG | + | chr8_4:12735417-12735436 | Msa1314190:CDS | 50.0% | |
| GCCTTTACCGATGGACGTTA+TGG | - | chr8_4:12735421-12735440 | None:intergenic | 50.0% | |
| GACGCTGCCGGAAATTTGAT+CGG | + | chr8_4:12735450-12735469 | Msa1314190:CDS | 50.0% | |
| GATACTGCCACTGCCTTCTT+AGG | + | chr8_4:12735504-12735523 | Msa1314190:CDS | 50.0% | |
| ATTACGGCCTAAGAAGGCAG+TGG | - | chr8_4:12735514-12735533 | None:intergenic | 50.0% | |
| TCAAATTTCCGGCAGCGTCG+AGG | - | chr8_4:12735449-12735468 | None:intergenic | 55.0% | |
| GCTGCCGGAAATTTGATCGG+AGG | + | chr8_4:12735453-12735472 | Msa1314190:CDS | 55.0% | |
| GGAGGAGTCACCAGCATTGT+TGG | + | chr8_4:12735471-12735490 | Msa1314190:CDS | 55.0% | |
| GGAGTCACCAGCATTGTTGG+TGG | + | chr8_4:12735474-12735493 | Msa1314190:CDS | 55.0% | |
| CCAACTGAGCTAAGCTCACG+AGG | - | chr8_4:12735739-12735758 | None:intergenic | 55.0% | |
| CCTCGTGAGCTTAGCTCAGT+TGG | + | chr8_4:12735736-12735755 | Msa1314190:intron | 55.0% | |
| TCAAATTTCCGGCAGCGTCG+AGG | - | chr8_4:12735449-12735468 | None:intergenic | 55.0% | |
| GCTGCCGGAAATTTGATCGG+AGG | + | chr8_4:12735453-12735472 | Msa1314190:CDS | 55.0% | |
| GGAGGAGTCACCAGCATTGT+TGG | + | chr8_4:12735471-12735490 | Msa1314190:CDS | 55.0% | |
| GGAGTCACCAGCATTGTTGG+TGG | + | chr8_4:12735474-12735493 | Msa1314190:CDS | 55.0% | |
| CCAACTGAGCTAAGCTCACG+AGG | - | chr8_4:12735739-12735758 | None:intergenic | 55.0% | |
| CCTCGTGAGCTTAGCTCAGT+TGG | + | chr8_4:12735736-12735755 | Msa1314190:intron | 55.0% | |
| GAGGACACCGCCTTTACCGA+TGG | - | chr8_4:12735430-12735449 | None:intergenic | 60.0% | |
| GAGGACACCGCCTTTACCGA+TGG | - | chr8_4:12735430-12735449 | None:intergenic | 60.0% | |
| GGCGGTGTCCTCGACGCTGC+CGG | + | chr8_4:12735438-12735457 | Msa1314190:CDS | 75.0% | |
| GGCGGTGTCCTCGACGCTGC+CGG | + | chr8_4:12735438-12735457 | Msa1314190:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 12735339 | 12736202 | 12735339 | ID=Msa1314190;Name=Msa1314190 |
| chr8_4 | mRNA | 12735339 | 12736202 | 12735339 | ID=Msa1314190-mRNA-1;Parent=Msa1314190;Name=Msa1314190-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0|0|0|0.5|1|1|2|0|88 |
| chr8_4 | exon | 12735339 | 12735570 | 12735339 | ID=Msa1314190-mRNA-1:exon:4784;Parent=Msa1314190-mRNA-1 |
| chr8_4 | exon | 12736168 | 12736202 | 12736168 | ID=Msa1314190-mRNA-1:exon:4785;Parent=Msa1314190-mRNA-1 |
| chr8_4 | CDS | 12735339 | 12735570 | 12735339 | ID=Msa1314190-mRNA-1:cds;Parent=Msa1314190-mRNA-1 |
| chr8_4 | CDS | 12736168 | 12736202 | 12736168 | ID=Msa1314190-mRNA-1:cds;Parent=Msa1314190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
