AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0145260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 6 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAAGCTCTGAAAGATTT+AGG 0.181064 1_4:-4943180 Msa0145260:CDS
AAAATCAAAGATGAATTATA+AGG 0.345874 1_4:-4943226 Msa0145260:CDS
AGGTTTGTACAGAAAAGTAA+TGG 0.506300 1_4:-4943259 Msa0145260:CDS
TACAAACCTAAATCATTCAG+AGG 0.592378 1_4:+4943273 None:intergenic
GATGAATTATAAGGACGCGA+AGG 0.618186 1_4:-4943217 Msa0145260:CDS
TAAGGACGCGAAGGATGATG+AGG 0.629874 1_4:-4943208 Msa0145260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCAAAGATGAATTATA+AGG - chr1_4:4943223-4943242 Msa0145260:CDS 15.0%
! AAAAAACCTCTGAATGATTT+AGG - chr1_4:4943170-4943189 Msa0145260:CDS 25.0%
TACAAACCTAAATCATTCAG+AGG + chr1_4:4943179-4943198 None:intergenic 30.0%
AGGTTTGTACAGAAAAGTAA+TGG - chr1_4:4943190-4943209 Msa0145260:CDS 30.0%
!! AAGAAAGCTCTGAAAGATTT+AGG - chr1_4:4943269-4943288 Msa0145260:CDS 30.0%
GATGAATTATAAGGACGCGA+AGG - chr1_4:4943232-4943251 Msa0145260:CDS 40.0%
TAAGGACGCGAAGGATGATG+AGG - chr1_4:4943241-4943260 Msa0145260:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 4943164 4943307 4943164 ID=Msa0145260;Name=Msa0145260
chr1_4 mRNA 4943164 4943307 4943164 ID=Msa0145260-mRNA-1;Parent=Msa0145260;Name=Msa0145260-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|47
chr1_4 exon 4943164 4943307 4943164 ID=Msa0145260-mRNA-1:exon:2071;Parent=Msa0145260-mRNA-1
chr1_4 CDS 4943164 4943307 4943164 ID=Msa0145260-mRNA-1:cds;Parent=Msa0145260-mRNA-1
Gene Sequence

>Msa0145260

ATGAAGAAAAAACCTCTGAATGATTTAGGTTTGTACAGAAAAGTAATGGAAATATTAGAAAAATCAAAGATGAATTATAAGGACGCGAAGGATGATGAGGAGATCAAGAAAGCTCTGAAAGATTTAGGTTTGTACAATCCATGA

Protein sequence

>Msa0145260

MKKKPLNDLGLYRKVMEILEKSKMNYKDAKDDEEIKKALKDLGLYNP*