AlfalfaGEDB | Msa0026450

M. sativa cultivar zhongmu-4 / Msa0026450

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003300	Msa0026450	0.800450	1.433058e-48	-8.615850e-47
Msa0022000	Msa0026450	0.803108	4.080177e-49	-8.615850e-47
Msa0024620	Msa0026450	0.801099	1.056554e-48	-8.615850e-47
Msa0026450	Msa0029850	0.810796	9.643569e-51	-8.615850e-47
Msa0026450	Msa0042550	0.825608	4.272473e-54	-8.615850e-47
Msa0026450	Msa0044260	0.811049	8.502215e-51	-8.615850e-47
Msa0026450	Msa0072120	0.822871	1.878136e-53	-8.615850e-47
Msa0026450	Msa0075880	0.815623	8.408186e-52	-8.615850e-47
Msa0026450	Msa0120490	0.829942	3.882192e-55	-8.615850e-47
Msa0026450	Msa0140500	-0.801408	9.133711e-49	-8.615850e-47
Msa0026450	Msa0142220	0.835336	1.780161e-56	-8.615850e-47
Msa0026450	Msa0145260	0.990960	3.484239e-185	-8.615850e-47
Msa0026450	Msa0165510	0.831432	1.675107e-55	-8.615850e-47
Msa0026450	Msa0165520	0.974026	1.948982e-137	-8.615850e-47
Msa0026450	Msa0165550	0.801651	8.143384e-49	-8.615850e-47
Msa0026450	Msa0169820	0.811250	7.690427e-51	-8.615850e-47
Msa0026450	Msa0178510	0.821986	3.015491e-53	-8.615850e-47
Msa0026450	Msa0190530	0.805663	1.197484e-49	-8.615850e-47
Msa0026450	Msa0202800	0.810429	1.157859e-50	-8.615850e-47
Msa0026450	Msa0202880	0.818626	1.776732e-52	-8.615850e-47
Msa0026450	Msa0212040	0.823793	1.144118e-53	-8.615850e-47
Msa0026450	Msa0213040	0.804275	2.335323e-49	-8.615850e-47
Msa0026450	Msa0214300	0.803702	3.072895e-49	-8.615850e-47
Msa0026450	Msa0214370	0.805797	1.122053e-49	-8.615850e-47
Msa0026450	Msa0214380	0.803438	3.485594e-49	-8.615850e-47
Msa0026450	Msa0218370	0.809899	1.506317e-50	-8.615850e-47
Msa0026450	Msa0229590	0.840907	6.552948e-58	-8.615850e-47
Msa0026450	Msa0233080	0.806458	8.147932e-50	-8.615850e-47
Msa0026450	Msa0241140	0.800623	1.321245e-48	-8.615850e-47
Msa0026450	Msa0253340	0.801610	8.300732e-49	-8.615850e-47
Msa0026450	Msa0273100	0.811668	6.239293e-51	-8.615850e-47
Msa0026450	Msa0285450	0.826344	2.856657e-54	-8.615850e-47
Msa0026450	Msa0290870	0.825844	3.756504e-54	-8.615850e-47
Msa0026450	Msa0292930	0.804559	2.038174e-49	-8.615850e-47
Msa0026450	Msa0305190	0.814909	1.211461e-51	-8.615850e-47
Msa0026450	Msa0308460	0.813665	2.281650e-51	-8.615850e-47
Msa0026450	Msa0330390	0.805109	1.564582e-49	-8.615850e-47
Msa0026450	Msa0344690	-0.821218	4.537880e-53	-8.615850e-47
Msa0026450	Msa0365780	0.800937	1.139813e-48	-8.615850e-47
Msa0026450	Msa0367870	0.805076	1.589390e-49	-8.615850e-47
Msa0026450	Msa0381610	-0.809608	1.739193e-50	-8.615850e-47
Msa0026450	Msa0389770	0.801060	1.075908e-48	-8.615850e-47
Msa0026450	Msa0399300	0.830607	2.670454e-55	-8.615850e-47
Msa0026450	Msa0419980	0.831670	1.463687e-55	-8.615850e-47
Msa0026450	Msa0433410	-0.819610	1.061052e-52	-8.615850e-47
Msa0026450	Msa0436800	0.801321	9.513688e-49	-8.615850e-47
Msa0026450	Msa0455760	0.812645	3.820835e-51	-8.615850e-47
Msa0026450	Msa0455900	0.803686	3.096622e-49	-8.615850e-47
Msa0026450	Msa0461790	0.803441	3.480799e-49	-8.615850e-47
Msa0026450	Msa0469940	-0.818521	1.876812e-52	-8.615850e-47
Msa0026450	Msa0479340	0.812162	4.870642e-51	-8.615850e-47
Msa0026450	Msa0518380	0.808087	3.679320e-50	-8.615850e-47
Msa0026450	Msa0521630	0.803511	3.366001e-49	-8.615850e-47
Msa0026450	Msa0537500	0.833298	5.779013e-56	-8.615850e-47
Msa0026450	Msa0544240	0.804557	2.040662e-49	-8.615850e-47
Msa0026450	Msa0559500	0.800224	1.593133e-48	-8.615850e-47
Msa0026450	Msa0560660	0.809822	1.564679e-50	-8.615850e-47
Msa0026450	Msa0600700	0.802349	5.852242e-49	-8.615850e-47
Msa0026450	Msa0603370	0.805763	1.140976e-49	-8.615850e-47
Msa0026450	Msa0622170	-0.805392	1.365162e-49	-8.615850e-47
Msa0026450	Msa0624960	0.803184	3.934435e-49	-8.615850e-47
Msa0026450	Msa0639270	0.820480	6.709512e-53	-8.615850e-47
Msa0026450	Msa0642730	0.820466	6.759373e-53	-8.615850e-47
Msa0026450	Msa0644400	0.804507	2.090251e-49	-8.615850e-47
Msa0026450	Msa0650300	0.804239	2.376417e-49	-8.615850e-47
Msa0026450	Msa0653660	0.804056	2.594652e-49	-8.615850e-47
Msa0026450	Msa0672690	0.800278	1.553192e-48	-8.615850e-47
Msa0026450	Msa0680660	0.804424	2.174459e-49	-8.615850e-47
Msa0026450	Msa0697030	0.800313	1.528208e-48	-8.615850e-47
Msa0026450	Msa0697040	0.808952	2.404523e-50	-8.615850e-47
Msa0026450	Msa0711830	0.816568	5.170403e-52	-8.615850e-47
Msa0026450	Msa0724210	0.820061	8.368895e-53	-8.615850e-47
Msa0026450	Msa0725450	0.813840	2.087452e-51	-8.615850e-47
Msa0026450	Msa0729800	0.834022	3.810086e-56	-8.615850e-47
Msa0026450	Msa0733290	0.801486	8.803297e-49	-8.615850e-47
Msa0026450	Msa0760130	0.804186	2.437612e-49	-8.615850e-47
Msa0026450	Msa0762360	0.815375	9.545915e-52	-8.615850e-47
Msa0026450	Msa0766090	0.811988	5.315920e-51	-8.615850e-47
Msa0026450	Msa0766630	0.846668	1.879467e-59	-8.615850e-47
Msa0026450	Msa0807030	0.813353	2.672014e-51	-8.615850e-47
Msa0026450	Msa0811080	0.819029	1.438886e-52	-8.615850e-47
Msa0026450	Msa0817460	0.816378	5.703593e-52	-8.615850e-47
Msa0026450	Msa0818110	0.801846	7.425307e-49	-8.615850e-47
Msa0026450	Msa0830230	0.802064	6.698450e-49	-8.615850e-47
Msa0026450	Msa0830510	0.815250	1.017959e-51	-8.615850e-47
Msa0026450	Msa0831850	0.807759	4.320617e-50	-8.615850e-47
Msa0026450	Msa0832340	0.804397	2.203513e-49	-8.615850e-47
Msa0026450	Msa0840380	0.813299	2.745738e-51	-8.615850e-47
Msa0026450	Msa0848960	0.801594	8.363023e-49	-8.615850e-47
Msa0026450	Msa0861130	0.800250	1.573934e-48	-8.615850e-47
Msa0026450	Msa0879550	-0.803122	4.053140e-49	-8.615850e-47
Msa0026450	Msa0884740	0.826776	2.253177e-54	-8.615850e-47
Msa0026450	Msa0885190	0.808595	2.867407e-50	-8.615850e-47
Msa0026450	Msa0927380	0.815070	1.116094e-51	-8.615850e-47
Msa0026450	Msa0928130	0.813806	2.123987e-51	-8.615850e-47
Msa0026450	Msa0935910	0.829743	4.340457e-55	-8.615850e-47
Msa0026450	Msa0939250	0.816486	5.393606e-52	-8.615850e-47
Msa0026450	Msa0946280	-0.804970	1.672755e-49	-8.615850e-47
Msa0026450	Msa0949790	0.808089	3.675259e-50	-8.615850e-47
Msa0026450	Msa0964900	0.805696	1.178500e-49	-8.615850e-47
Msa0026450	Msa0968420	0.815493	8.989271e-52	-8.615850e-47
Msa0026450	Msa0969450	0.818540	1.858486e-52	-8.615850e-47
Msa0026450	Msa0969600	0.802667	5.032728e-49	-8.615850e-47
Msa0026450	Msa0986660	0.808504	2.998472e-50	-8.615850e-47
Msa0026450	Msa1000060	0.814045	1.881657e-51	-8.615850e-47
Msa0026450	Msa1002330	0.838081	3.553808e-57	-8.615850e-47
Msa0026450	Msa1016320	0.805871	1.082908e-49	-8.615850e-47
Msa0026450	Msa1023050	0.837156	6.137768e-57	-8.615850e-47
Msa0026450	Msa1023950	0.823379	1.429770e-53	-8.615850e-47
Msa0026450	Msa1039710	-0.811006	8.687752e-51	-8.615850e-47
Msa0026450	Msa1065570	0.810756	9.837717e-51	-8.615850e-47
Msa0026450	Msa1075650	0.803345	3.643852e-49	-8.615850e-47
Msa0026450	Msa1091900	0.808082	3.687777e-50	-8.615850e-47
Msa0026450	Msa1121480	0.837509	4.983204e-57	-8.615850e-47
Msa0026450	Msa1121490	0.831065	2.061303e-55	-8.615850e-47
Msa0026450	Msa1122520	0.817055	4.021997e-52	-8.615850e-47
Msa0026450	Msa1135220	0.804029	2.628310e-49	-8.615850e-47
Msa0026450	Msa1144720	-0.818165	2.259613e-52	-8.615850e-47
Msa0026450	Msa1188990	0.808629	2.819215e-50	-8.615850e-47
Msa0026450	Msa1242300	0.812453	4.208062e-51	-8.615850e-47
Msa0026450	Msa1255330	0.800341	1.508129e-48	-8.615850e-47
Msa0026450	Msa1257800	-0.812363	4.403423e-51	-8.615850e-47
Msa0026450	Msa1268880	0.814158	1.776124e-51	-8.615850e-47
Msa0026450	Msa1304890	-0.802261	6.102598e-49	-8.615850e-47
Msa0026450	Msa1315420	0.827920	1.198072e-54	-8.615850e-47
Msa0026450	Msa1320300	0.814033	1.893058e-51	-8.615850e-47
Msa0026450	Msa1325860	-0.807901	4.029727e-50	-8.615850e-47
Msa0026450	Msa1372150	0.830386	3.024689e-55	-8.615850e-47
Msa0026450	Msa1376930	0.802015	6.855667e-49	-8.615850e-47
Msa0026450	Msa1378040	0.808066	3.717032e-50	-8.615850e-47
Msa0026450	Msa1378940	0.808594	2.868479e-50	-8.615850e-47
Msa0026450	Msa1381530	0.813060	3.098981e-51	-8.615850e-47
Msa0026450	Msa1383100	0.809110	2.224712e-50	-8.615850e-47
Msa0026450	Msa1389200	0.845983	2.889593e-59	-8.615850e-47
Msa0026450	Msa1394380	0.807512	4.875908e-50	-8.615850e-47
Msa0026450	Msa1408340	0.806825	6.813994e-50	-8.615850e-47
Msa0026450	Msa1448560	0.814638	1.391452e-51	-8.615850e-47
Msa0026450	Msa1453250	0.836355	9.821520e-57	-8.615850e-47
Msa0026450	Msa1459050	0.806230	9.097453e-50	-8.615850e-47
Msa0026450	Msa1463900	0.804826	1.792590e-49	-8.615850e-47
Msa0026450	Msa1466540	0.804998	1.650504e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 7 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAAGCTCTGAAAGATTT+AGG	0.181064	1_1:-50304430	Msa0026450:CDS
ATCGAAGACTTTATAAAGAA+TGG	0.344331	1_1:+50304556	None:intergenic
AAAATCAAAGATGAATTATA+AGG	0.345874	1_1:-50304476	Msa0026450:CDS
AGGTTTGTACAGAAAAGTAA+TGG	0.506300	1_1:-50304509	Msa0026450:CDS
TACAAACCTAAATCATTCAG+AGG	0.592378	1_1:+50304523	None:intergenic
GATGAATTATAAGGACGCGA+AGG	0.618186	1_1:-50304467	Msa0026450:CDS
TAAGGACGCGAAGGATGATG+AGG	0.629874	1_1:-50304458	Msa0026450:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATCAAAGATGAATTATA+AGG	-	chr1_1:50304705-50304724	Msa0026450:intron	15.0%
!!	ATAAGTTTAACCTATTTACA+TGG	+	chr1_1:50304485-50304504	None:intergenic	20.0%
!	TATTTAGCTATAACTGAAGT+AGG	-	chr1_1:50304502-50304521	Msa0026450:CDS	25.0%
!	ATCGAAGACTTTATAAAGAA+TGG	+	chr1_1:50304628-50304647	None:intergenic	25.0%
!	AAAAAACCTCTGAATGATTT+AGG	-	chr1_1:50304652-50304671	Msa0026450:intron	25.0%
	AGTTTAACCTATTTACATGG+AGG	+	chr1_1:50304482-50304501	None:intergenic	30.0%
	TACAAACCTAAATCATTCAG+AGG	+	chr1_1:50304661-50304680	None:intergenic	30.0%
	AGGTTTGTACAGAAAAGTAA+TGG	-	chr1_1:50304672-50304691	Msa0026450:intron	30.0%
!!	AAGAAAGCTCTGAAAGATTT+AGG	-	chr1_1:50304751-50304770	Msa0026450:intron	30.0%
	TGCTATTCCTCCATGTAAAT+AGG	-	chr1_1:50304472-50304491	Msa0026450:CDS	35.0%
	ACAATTTAGCAGGATAATGC+AGG	+	chr1_1:50304530-50304549	None:intergenic	35.0%
	GCATTATCCTGCTAAATTGT+AGG	-	chr1_1:50304530-50304549	Msa0026450:CDS	35.0%
	ACTAACTCCTACAATTTAGC+AGG	+	chr1_1:50304540-50304559	None:intergenic	35.0%
	GAGGAATAGCAGCAACTATA+AGG	+	chr1_1:50304463-50304482	None:intergenic	40.0%
	GATGAATTATAAGGACGCGA+AGG	-	chr1_1:50304714-50304733	Msa0026450:intron	40.0%
	TAAGGACGCGAAGGATGATG+AGG	-	chr1_1:50304723-50304742	Msa0026450:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	50304414	50304789	50304414	ID=Msa0026450;Name=Msa0026450
chr1_1	mRNA	50304414	50304789	50304414	ID=Msa0026450-mRNA-1;Parent=Msa0026450;Name=Msa0026450-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0\|0\|0\|0.5\|0\|0.5\|2\|0\|64
chr1_1	exon	50304779	50304789	50304779	ID=Msa0026450-mRNA-1:exon:14632;Parent=Msa0026450-mRNA-1
chr1_1	exon	50304414	50304597	50304414	ID=Msa0026450-mRNA-1:exon:14631;Parent=Msa0026450-mRNA-1
chr1_1	CDS	50304779	50304789	50304779	ID=Msa0026450-mRNA-1:cds;Parent=Msa0026450-mRNA-1
chr1_1	CDS	50304414	50304597	50304414	ID=Msa0026450-mRNA-1:cds;Parent=Msa0026450-mRNA-1

Gene Sequence	>Msa0026450 ATGGATCTCAATAGTTTTGAATACATAAAGCCATTCTTTATAAAGTCTTCGATGAAGAAAAAACCTCTGAATGATTTAGGTTTGTACAGAAAAGTAATGGAAATATTAGAAAAATCAAAGATGAATTATAAGGACGCGAAGGATGATGAGGAGATCAAGAAAGCTCTGAAAGATTTAGGTTTGTACAATCCATGA
Protein sequence	>Msa0026450 MDLNSFEYIKPFFIKSSMKKKPLNDLGLYRKVMEILEKSKMNYKDAKDDEEIKKALKDLGLYNP*