Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026450 | Msa0165520 | 0.974026 | 1.948982e-137 | -8.615850e-47 |
| Msa0029850 | Msa0165520 | 0.812473 | 4.165806e-51 | -8.615850e-47 |
| Msa0042550 | Msa0165520 | 0.828023 | 1.132115e-54 | -8.615850e-47 |
| Msa0050820 | Msa0165520 | 0.805560 | 1.258633e-49 | -8.615850e-47 |
| Msa0072120 | Msa0165520 | 0.807261 | 5.511257e-50 | -8.615850e-47 |
| Msa0075880 | Msa0165520 | 0.823221 | 1.556196e-53 | -8.615850e-47 |
| Msa0120490 | Msa0165520 | 0.803926 | 2.761160e-49 | -8.615850e-47 |
| Msa0140500 | Msa0165520 | -0.806713 | 7.197554e-50 | -8.615850e-47 |
| Msa0142220 | Msa0165520 | 0.809974 | 1.451065e-50 | -8.615850e-47 |
| Msa0145260 | Msa0165520 | 0.965808 | 4.351776e-125 | -8.615850e-47 |
| Msa0165510 | Msa0165520 | 0.834971 | 2.201701e-56 | -8.615850e-47 |
| Msa0165520 | Msa0169820 | 0.808014 | 3.814255e-50 | -8.615850e-47 |
| Msa0165520 | Msa0178510 | 0.824436 | 8.079432e-54 | -8.615850e-47 |
| Msa0165520 | Msa0212040 | 0.814950 | 1.186735e-51 | -8.615850e-47 |
| Msa0165520 | Msa0214300 | 0.802091 | 6.613311e-49 | -8.615850e-47 |
| Msa0165520 | Msa0218370 | 0.809408 | 1.920094e-50 | -8.615850e-47 |
| Msa0165520 | Msa0218810 | 0.801779 | 7.663512e-49 | -8.615850e-47 |
| Msa0165520 | Msa0225220 | 0.801264 | 9.775872e-49 | -8.615850e-47 |
| Msa0165520 | Msa0229590 | 0.828507 | 8.651919e-55 | -8.615850e-47 |
| Msa0165520 | Msa0241140 | 0.808221 | 3.444963e-50 | -8.615850e-47 |
| Msa0165520 | Msa0285450 | 0.819318 | 1.237123e-52 | -8.615850e-47 |
| Msa0165520 | Msa0290870 | 0.810855 | 9.367976e-51 | -8.615850e-47 |
| Msa0165520 | Msa0305190 | 0.809665 | 1.690894e-50 | -8.615850e-47 |
| Msa0165520 | Msa0308460 | 0.812124 | 4.964365e-51 | -8.615850e-47 |
| Msa0165520 | Msa0340450 | 0.805333 | 1.404225e-49 | -8.615850e-47 |
| Msa0165520 | Msa0344690 | -0.814455 | 1.527022e-51 | -8.615850e-47 |
| Msa0165520 | Msa0363790 | 0.802323 | 5.925524e-49 | -8.615850e-47 |
| Msa0165520 | Msa0367870 | 0.802521 | 5.394017e-49 | -8.615850e-47 |
| Msa0165520 | Msa0381610 | -0.814183 | 1.753728e-51 | -8.615850e-47 |
| Msa0165520 | Msa0392040 | -0.805167 | 1.521260e-49 | -8.615850e-47 |
| Msa0165520 | Msa0399300 | 0.816627 | 5.015526e-52 | -8.615850e-47 |
| Msa0165520 | Msa0411390 | 0.810536 | 1.097691e-50 | -8.615850e-47 |
| Msa0165520 | Msa0419980 | 0.830830 | 2.354379e-55 | -8.615850e-47 |
| Msa0165520 | Msa0425170 | 0.800732 | 1.255167e-48 | -8.615850e-47 |
| Msa0165520 | Msa0433410 | -0.822047 | 2.918950e-53 | -8.615850e-47 |
| Msa0165520 | Msa0455760 | 0.814481 | 1.507233e-51 | -8.615850e-47 |
| Msa0165520 | Msa0461790 | 0.815374 | 9.552208e-52 | -8.615850e-47 |
| Msa0165520 | Msa0469940 | -0.810008 | 1.427103e-50 | -8.615850e-47 |
| Msa0165520 | Msa0479340 | 0.805257 | 1.456790e-49 | -8.615850e-47 |
| Msa0165520 | Msa0503430 | 0.803993 | 2.673209e-49 | -8.615850e-47 |
| Msa0165520 | Msa0518380 | 0.802841 | 4.632381e-49 | -8.615850e-47 |
| Msa0165520 | Msa0521630 | 0.804092 | 2.550172e-49 | -8.615850e-47 |
| Msa0165520 | Msa0537500 | 0.835643 | 1.489309e-56 | -8.615850e-47 |
| Msa0165520 | Msa0543270 | 0.805663 | 1.197339e-49 | -8.615850e-47 |
| Msa0165520 | Msa0622170 | -0.800767 | 1.234801e-48 | -8.615850e-47 |
| Msa0165520 | Msa0639270 | 0.810746 | 9.886745e-51 | -8.615850e-47 |
| Msa0165520 | Msa0642730 | 0.810671 | 1.026510e-50 | -8.615850e-47 |
| Msa0165520 | Msa0653660 | 0.801255 | 9.813518e-49 | -8.615850e-47 |
| Msa0165520 | Msa0672690 | 0.801488 | 8.794711e-49 | -8.615850e-47 |
| Msa0165520 | Msa0680660 | 0.810756 | 9.838204e-51 | -8.615850e-47 |
| Msa0165520 | Msa0697040 | 0.809014 | 2.332858e-50 | -8.615850e-47 |
| Msa0165520 | Msa0703780 | 0.800440 | 1.440056e-48 | -8.615850e-47 |
| Msa0165520 | Msa0704920 | 0.804150 | 2.479900e-49 | -8.615850e-47 |
| Msa0165520 | Msa0711830 | 0.819422 | 1.171007e-52 | -8.615850e-47 |
| Msa0165520 | Msa0724210 | 0.824782 | 6.698933e-54 | -8.615850e-47 |
| Msa0165520 | Msa0725450 | 0.801970 | 7.003375e-49 | -8.615850e-47 |
| Msa0165520 | Msa0729800 | 0.811009 | 8.673642e-51 | -8.615850e-47 |
| Msa0165520 | Msa0731940 | 0.807021 | 6.193878e-50 | -8.615850e-47 |
| Msa0165520 | Msa0749440 | 0.800442 | 1.438591e-48 | -8.615850e-47 |
| Msa0165520 | Msa0762360 | 0.822681 | 2.079795e-53 | -8.615850e-47 |
| Msa0165520 | Msa0766090 | 0.803941 | 2.741192e-49 | -8.615850e-47 |
| Msa0165520 | Msa0766630 | 0.822271 | 2.590204e-53 | -8.615850e-47 |
| Msa0165520 | Msa0769040 | 0.809006 | 2.342010e-50 | -8.615850e-47 |
| Msa0165520 | Msa0807030 | 0.807135 | 5.858671e-50 | -8.615850e-47 |
| Msa0165520 | Msa0817460 | 0.815544 | 8.756838e-52 | -8.615850e-47 |
| Msa0165520 | Msa0830510 | 0.817850 | 2.662132e-52 | -8.615850e-47 |
| Msa0165520 | Msa0831850 | 0.810607 | 1.059569e-50 | -8.615850e-47 |
| Msa0165520 | Msa0832340 | 0.802253 | 6.125902e-49 | -8.615850e-47 |
| Msa0165520 | Msa0840380 | 0.803521 | 3.350709e-49 | -8.615850e-47 |
| Msa0165520 | Msa0884740 | 0.818035 | 2.417981e-52 | -8.615850e-47 |
| Msa0165520 | Msa0898750 | 0.810394 | 1.177955e-50 | -8.615850e-47 |
| Msa0165520 | Msa0927380 | 0.802071 | 6.674885e-49 | -8.615850e-47 |
| Msa0165520 | Msa0928130 | 0.826708 | 2.338467e-54 | -8.615850e-47 |
| Msa0165520 | Msa0935910 | 0.811546 | 6.633969e-51 | -8.615850e-47 |
| Msa0165520 | Msa0939250 | 0.806949 | 6.416805e-50 | -8.615850e-47 |
| Msa0165520 | Msa0946280 | -0.807213 | 5.642449e-50 | -8.615850e-47 |
| Msa0165520 | Msa0949790 | 0.804519 | 2.077460e-49 | -8.615850e-47 |
| Msa0165520 | Msa0965610 | 0.802739 | 4.863408e-49 | -8.615850e-47 |
| Msa0165520 | Msa0968420 | 0.810147 | 1.331578e-50 | -8.615850e-47 |
| Msa0165520 | Msa0969450 | 0.812241 | 4.681478e-51 | -8.615850e-47 |
| Msa0165520 | Msa0969600 | 0.806431 | 8.253594e-50 | -8.615850e-47 |
| Msa0165520 | Msa0986660 | 0.805544 | 1.268195e-49 | -8.615850e-47 |
| Msa0165520 | Msa1002330 | 0.815068 | 1.117071e-51 | -8.615850e-47 |
| Msa0165520 | Msa1016320 | 0.801652 | 8.137492e-49 | -8.615850e-47 |
| Msa0165520 | Msa1023050 | 0.813390 | 2.621976e-51 | -8.615850e-47 |
| Msa0165520 | Msa1023950 | 0.812711 | 3.694481e-51 | -8.615850e-47 |
| Msa0165520 | Msa1039710 | -0.806243 | 9.040220e-50 | -8.615850e-47 |
| Msa0165520 | Msa1065570 | 0.804336 | 2.268783e-49 | -8.615850e-47 |
| Msa0165520 | Msa1091900 | 0.802728 | 4.888040e-49 | -8.615850e-47 |
| Msa0165520 | Msa1121480 | 0.820944 | 5.248795e-53 | -8.615850e-47 |
| Msa0165520 | Msa1121490 | 0.809362 | 1.964683e-50 | -8.615850e-47 |
| Msa0165520 | Msa1122520 | 0.800122 | 1.670995e-48 | -8.615850e-47 |
| Msa0165520 | Msa1144720 | -0.808366 | 3.208798e-50 | -8.615850e-47 |
| Msa0165520 | Msa1255330 | 0.801122 | 1.044833e-48 | -8.615850e-47 |
| Msa0165520 | Msa1268880 | 0.818465 | 1.932329e-52 | -8.615850e-47 |
| Msa0165520 | Msa1315420 | 0.817301 | 3.539681e-52 | -8.615850e-47 |
| Msa0165520 | Msa1325860 | -0.813947 | 1.977174e-51 | -8.615850e-47 |
| Msa0165520 | Msa1344130 | 0.804733 | 1.874538e-49 | -8.615850e-47 |
| Msa0165520 | Msa1372150 | 0.825742 | 3.970969e-54 | -8.615850e-47 |
| Msa0165520 | Msa1374470 | 0.801264 | 9.772684e-49 | -8.615850e-47 |
| Msa0165520 | Msa1378940 | 0.805078 | 1.587932e-49 | -8.615850e-47 |
| Msa0165520 | Msa1381530 | 0.804925 | 1.709334e-49 | -8.615850e-47 |
| Msa0165520 | Msa1389200 | 0.838572 | 2.655769e-57 | -8.615850e-47 |
| Msa0165520 | Msa1394380 | 0.806360 | 8.544226e-50 | -8.615850e-47 |
| Msa0165520 | Msa1424680 | 0.802022 | 6.833965e-49 | -8.615850e-47 |
| Msa0165520 | Msa1445390 | -0.804898 | 1.731909e-49 | -8.615850e-47 |
| Msa0165520 | Msa1448560 | 0.810521 | 1.105797e-50 | -8.615850e-47 |
| Msa0165520 | Msa1449340 | -0.800038 | 1.738029e-48 | -8.615850e-47 |
| Msa0165520 | Msa1453250 | 0.839112 | 1.925291e-57 | -8.615850e-47 |
| Msa0165520 | Msa1463900 | 0.809233 | 2.094272e-50 | -8.615850e-47 |
| Msa0165520 | Msa1466540 | 0.808170 | 3.532820e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 6 sgRNAs with CRISPR-Local
Find 7 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAAGCTCTGAAAGATTT+AGG | 0.181064 | 1_4:-46137568 | Msa0165520:CDS |
| AAAATCAAAGATGAATTATA+AGG | 0.345874 | 1_4:-46137614 | Msa0165520:CDS |
| AGGTTTGTACAGAAAAGTAA+TGG | 0.506300 | 1_4:-46137647 | Msa0165520:CDS |
| TACAAACCTAAATCATTCAG+AGG | 0.592378 | 1_4:+46137661 | None:intergenic |
| GATGAATTATAAGGACGCGA+AGG | 0.618186 | 1_4:-46137605 | Msa0165520:CDS |
| TAAGGACGCGAAGGATGATG+AGG | 0.629874 | 1_4:-46137596 | Msa0165520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCAAAGATGAATTATA+AGG | - | chr1_4:46137611-46137630 | Msa0165520:CDS | 15.0% |
| ! | AAAAAACCTCTGAATGATTT+AGG | - | chr1_4:46137558-46137577 | Msa0165520:CDS | 25.0% |
| TACAAACCTAAATCATTCAG+AGG | + | chr1_4:46137567-46137586 | None:intergenic | 30.0% | |
| AGGTTTGTACAGAAAAGTAA+TGG | - | chr1_4:46137578-46137597 | Msa0165520:CDS | 30.0% | |
| !! | AAGAAAGCTCTGAAAGATTT+AGG | - | chr1_4:46137657-46137676 | Msa0165520:CDS | 30.0% |
| GATGAATTATAAGGACGCGA+AGG | - | chr1_4:46137620-46137639 | Msa0165520:CDS | 40.0% | |
| TAAGGACGCGAAGGATGATG+AGG | - | chr1_4:46137629-46137648 | Msa0165520:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 46137552 | 46137695 | 46137552 | ID=Msa0165520;Name=Msa0165520 |
| chr1_4 | mRNA | 46137552 | 46137695 | 46137552 | ID=Msa0165520-mRNA-1;Parent=Msa0165520;Name=Msa0165520-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|47 |
| chr1_4 | exon | 46137552 | 46137695 | 46137552 | ID=Msa0165520-mRNA-1:exon:13243;Parent=Msa0165520-mRNA-1 |
| chr1_4 | CDS | 46137552 | 46137695 | 46137552 | ID=Msa0165520-mRNA-1:cds;Parent=Msa0165520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
