AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1307730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307730 MtrunA17_Chr3g0122371 97.561 123 3 0 1 123 1 123 1.48e-88 252
Msa1307730 MtrunA17_Chr8g0377491 90.244 123 12 0 1 123 1 123 1.13e-81 235
Msa1307730 MtrunA17_Chr2g0308331 80.851 94 12 1 1 94 1 88 2.46e-49 153
Msa1307730 MtrunA17_Chr7g0235151 35.246 122 71 5 3 119 12 130 3.73e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307730 AT1G27970.1 82.114 123 22 0 1 123 4 126 5.30e-74 216
Msa1307730 AT1G27310.1 82.927 123 20 1 1 123 1 122 2.57e-73 214
Msa1307730 AT1G27970.2 81.356 118 22 0 1 118 4 121 1.22e-69 205
Msa1307730 AT1G11570.2 59.504 121 46 1 6 123 12 132 9.42e-47 147
Msa1307730 AT1G11570.3 59.504 121 46 1 6 123 33 153 1.24e-46 147
Msa1307730 AT1G11570.1 57.426 101 40 1 6 103 12 112 1.57e-34 116
Msa1307730 AT1G11570.4 57.426 101 40 1 6 103 33 133 2.59e-34 116
Msa1307730 AT5G48650.1 37.302 126 70 6 1 120 11 133 2.73e-12 62.4

Find 68 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTTGCATCTAAAATT+TGG 0.111315 8_4:+3654639 Msa1307730:three_prime_UTR
CAGACATTTAAAATTAAAAT+TGG 0.120296 8_4:+3654488 Msa1307730:three_prime_UTR
AGATGCAAATTGCATGAATA+TGG 0.182084 8_4:-3654630 None:intergenic
CTTTGAAGGTCAGCAAATTA+TGG 0.205195 8_4:+3653112 Msa1307730:CDS
AAAATTAAAATTGGGTCTTT+TGG 0.234324 8_4:+3654497 Msa1307730:three_prime_UTR
TGTTGAAGATCAATGAATAT+TGG 0.239280 8_4:+3654584 Msa1307730:three_prime_UTR
GTTGAAGATCAATGAATATT+GGG 0.271194 8_4:+3654585 Msa1307730:three_prime_UTR
ATTGCTTGGATATATATAAT+TGG 0.283570 8_4:+3654768 Msa1307730:three_prime_UTR
TTTAATTTCTGCTTAGTGTT+TGG 0.317853 8_4:+3654967 Msa1307730:three_prime_UTR
CAGCGACAATTCAAATTTAG+AGG 0.334225 8_4:+3654837 Msa1307730:three_prime_UTR
ATACAAGACTTGCTTTAGAT+TGG 0.359930 8_4:-3654896 None:intergenic
TTTGAAGGTCAGCAAATTAT+GGG 0.362536 8_4:+3653113 Msa1307730:CDS
AGCCATTCCAATTGATTGCT+TGG 0.382527 8_4:+3654754 Msa1307730:three_prime_UTR
AATGCCTTTGACAATGCGTT+TGG 0.385167 8_4:-3653011 None:intergenic
ATTGTGTTTAAACTCTTTGA+AGG 0.385926 8_4:+3654555 Msa1307730:three_prime_UTR
CTCTATCTCACTCCTTGGCC+TGG 0.398404 8_4:-3652927 None:intergenic
GGTTCTATGCTCACCTTTGA+AGG 0.402799 8_4:+3653098 Msa1307730:CDS
CGTGTTGTTCGCCGGCGAGC+TGG 0.403242 8_4:-3653262 None:intergenic
TATATATCCAAGCAATCAAT+TGG 0.405869 8_4:-3654761 None:intergenic
CGCATTGTCAAAGGCATTCG+TGG 0.412022 8_4:+3653016 Msa1307730:CDS
CATAATTTGCTGACCTTCAA+AGG 0.412917 8_4:-3653111 None:intergenic
GTTTAAACACAATTCTAAAC+AGG 0.413628 8_4:-3654545 None:intergenic
AGACATTTAAAATTAAAATT+GGG 0.414797 8_4:+3654489 Msa1307730:three_prime_UTR
CTTGCGGTGTTGGTATCAAA+TGG 0.429115 8_4:-3654370 None:intergenic
TTGGTAGAGAGTGGCGAGAT+TGG 0.433392 8_4:-3653072 None:intergenic
TGGTAGAGAGTGGCGAGATT+GGG 0.451654 8_4:-3653071 None:intergenic
TTCTTCACGCATAGTTCAAA+CGG 0.464644 8_4:-3654421 None:intergenic
TGATGGAGTGATGGCACTGC+TGG 0.466864 8_4:-3653175 None:intergenic
AATTGACCTGACTGAATTTG+AGG 0.471209 8_4:-3653286 None:intergenic
ATTTGATTTCATTGCTGAGT+TGG 0.473341 8_4:+3654699 Msa1307730:three_prime_UTR
GAGCTTATTTATAAGAAGCC+AGG 0.479694 8_4:+3652909 Msa1307730:five_prime_UTR
TTTGAGGGCGTGTTGTTCGC+CGG 0.484969 8_4:-3653270 None:intergenic
TTGAAGATCAATGAATATTG+GGG 0.510000 8_4:+3654586 Msa1307730:three_prime_UTR
ACCGTCGATTGTCAACCTTC+TGG 0.514517 8_4:+3653200 Msa1307730:CDS
ATTGGGACGGTTGGTGTCGA+AGG 0.521383 8_4:-3653054 None:intergenic
GAGTGATGGCACTGCTGGAA+AGG 0.524413 8_4:-3653170 None:intergenic
ATTATTGCAGTGTCATGCTT+AGG 0.526314 8_4:+3654936 Msa1307730:three_prime_UTR
TTGACAATCGACGGTGGTGA+TGG 0.528680 8_4:-3653192 None:intergenic
TAATAGCTTCCTTGCGGTGT+TGG 0.531758 8_4:-3654380 None:intergenic
ATTGACCTGACTGAATTTGA+GGG 0.536270 8_4:-3653285 None:intergenic
AGTCTCTCTATCTCACTCCT+TGG 0.546043 8_4:-3652932 None:intergenic
ATTTATAAGAAGCCAGGCCA+AGG 0.547142 8_4:+3652915 Msa1307730:five_prime_UTR
ATGCCGCCGTTAGCGCCAGA+AGG 0.549119 8_4:-3653215 None:intergenic
ATGGCTAGCACACTTTGTGC+TGG 0.551762 8_4:-3654737 None:intergenic
TGTCAACCTTCTGGCGCTAA+CGG 0.557071 8_4:+3653209 Msa1307730:CDS
AGTGGCGAGATTGGGACGGT+TGG 0.563337 8_4:-3653063 None:intergenic
ATCCAAGCAATCAATTGGAA+TGG 0.565549 8_4:-3654756 None:intergenic
GGGACGGTTGGTGTCGAAGG+TGG 0.565989 8_4:-3653051 None:intergenic
AGGTGAGCATAGAACCATCT+TGG 0.568405 8_4:-3653091 None:intergenic
CTCGCCACTCTCTACCAAGA+TGG 0.569246 8_4:+3653077 Msa1307730:CDS
ACACGCCCTCAAATTCAGTC+AGG 0.570302 8_4:+3653280 Msa1307730:CDS
CAGAGAAAGAGATCGTGTTA+CGG 0.571732 8_4:+3652969 Msa1307730:five_prime_UTR
GGATCCAAACGCATTGTCAA+AGG 0.574827 8_4:+3653007 Msa1307730:CDS
ACTTTGTGCTGGCTACATAA+CGG 0.577022 8_4:-3654726 None:intergenic
TTACGGCGGCGTAAGAGCGA+TGG 0.580005 8_4:+3652986 Msa1307730:exon
AGTGGTAATCTCCAGCTCGC+CGG 0.581878 8_4:+3653251 Msa1307730:CDS
AGTGATGGCACTGCTGGAAA+GGG 0.591260 8_4:-3653169 None:intergenic
GGCATGCTTGTCTTTGTCAG+TGG 0.596448 8_4:+3653233 Msa1307730:CDS
CATTTGATACCAACACCGCA+AGG 0.621557 8_4:+3654371 Msa1307730:CDS
GCCAGAAGGTTGACAATCGA+CGG 0.623192 8_4:-3653201 None:intergenic
AGAACCATCTTGGTAGAGAG+TGG 0.624728 8_4:-3653081 None:intergenic
CGACGGTGGTGATGGAGTGA+TGG 0.635677 8_4:-3653184 None:intergenic
AACACATAATAGCTTCCTTG+CGG 0.638641 8_4:-3654386 None:intergenic
CAACCTTCTGGCGCTAACGG+CGG 0.643186 8_4:+3653212 Msa1307730:CDS
AGAGAGTGGCGAGATTGGGA+CGG 0.652017 8_4:-3653067 None:intergenic
AGAAAGAGATCGTGTTACGG+CGG 0.704735 8_4:+3652972 Msa1307730:five_prime_UTR
TGAAGATCAATGAATATTGG+GGG 0.706123 8_4:+3654587 Msa1307730:three_prime_UTR
AGAAGGTTGACAATCGACGG+TGG 0.721439 8_4:-3653198 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTATTTGTTAATTTTA+GGG + chr8_4:3653327-3653346 Msa1307730:intron 10.0%
!!! AGACATTTAAAATTAAAATT+GGG + chr8_4:3654489-3654508 Msa1307730:three_prime_UTR 10.0%
!!! TTTTCTATTTGTTAATTTTA+GGG + chr8_4:3653327-3653346 Msa1307730:intron 10.0%
!!! AGACATTTAAAATTAAAATT+GGG + chr8_4:3654489-3654508 Msa1307730:three_prime_UTR 10.0%
!!! GTTTTCTATTTGTTAATTTT+AGG + chr8_4:3653326-3653345 Msa1307730:intron 15.0%
!!! TTTCATGATTAAGTTTTAAT+TGG + chr8_4:3653401-3653420 Msa1307730:intron 15.0%
!!! GTATTATCAATATTCTAAAA+TGG + chr8_4:3653622-3653641 Msa1307730:intron 15.0%
!!! ATTAACATTTTACAATTCTA+AGG - chr8_4:3653649-3653668 None:intergenic 15.0%
!! AGAATTGTAAAATGTTAATT+AGG + chr8_4:3653650-3653669 Msa1307730:intron 15.0%
!!! TTTTATTTTAGTGATCATTT+TGG + chr8_4:3653893-3653912 Msa1307730:intron 15.0%
!! TTGATATAATCAACAATTTA+AGG + chr8_4:3653942-3653961 Msa1307730:intron 15.0%
!! TGATATAATCAACAATTTAA+GGG + chr8_4:3653943-3653962 Msa1307730:intron 15.0%
!! AAATCCATCATAATAATAAT+AGG - chr8_4:3653986-3654005 None:intergenic 15.0%
!!! TATTATTATTATGATGGATT+TGG + chr8_4:3653985-3654004 Msa1307730:intron 15.0%
!!! CAGACATTTAAAATTAAAAT+TGG + chr8_4:3654488-3654507 Msa1307730:three_prime_UTR 15.0%
!!! GTTTTCTATTTGTTAATTTT+AGG + chr8_4:3653326-3653345 Msa1307730:intron 15.0%
!!! TTTCATGATTAAGTTTTAAT+TGG + chr8_4:3653401-3653420 Msa1307730:intron 15.0%
!!! GTATTATCAATATTCTAAAA+TGG + chr8_4:3653622-3653641 Msa1307730:intron 15.0%
!!! ATTAACATTTTACAATTCTA+AGG - chr8_4:3653649-3653668 None:intergenic 15.0%
!! AGAATTGTAAAATGTTAATT+AGG + chr8_4:3653650-3653669 Msa1307730:intron 15.0%
!!! TTTTATTTTAGTGATCATTT+TGG + chr8_4:3653893-3653912 Msa1307730:intron 15.0%
!! TTGATATAATCAACAATTTA+AGG + chr8_4:3653942-3653961 Msa1307730:intron 15.0%
!! TGATATAATCAACAATTTAA+GGG + chr8_4:3653943-3653962 Msa1307730:intron 15.0%
!! AAATCCATCATAATAATAAT+AGG - chr8_4:3653986-3654005 None:intergenic 15.0%
!!! TATTATTATTATGATGGATT+TGG + chr8_4:3653985-3654004 Msa1307730:intron 15.0%
!!! CAGACATTTAAAATTAAAAT+TGG + chr8_4:3654488-3654507 Msa1307730:three_prime_UTR 15.0%
!! CATGAAAAACTTAAATAACA+CGG - chr8_4:3653388-3653407 None:intergenic 20.0%
!! ATAATACCAATCAAATTTCA+GGG - chr8_4:3653609-3653628 None:intergenic 20.0%
!!! TGGAATATTGTTATTGTAAA+TGG + chr8_4:3653913-3653932 Msa1307730:intron 20.0%
!! TTTGCCTATTATTATTATGA+TGG + chr8_4:3653979-3653998 Msa1307730:intron 20.0%
!!! TAGAGTGAAATTTCTAATTT+TGG + chr8_4:3654106-3654125 Msa1307730:intron 20.0%
!!! AAAATTAAAATTGGGTCTTT+TGG + chr8_4:3654497-3654516 Msa1307730:three_prime_UTR 20.0%
!! ATTGCTTGGATATATATAAT+TGG + chr8_4:3654768-3654787 Msa1307730:three_prime_UTR 20.0%
!!! CAATAGTATTTTAAAAGAGA+AGG - chr8_4:3654805-3654824 None:intergenic 20.0%
!! CATGAAAAACTTAAATAACA+CGG - chr8_4:3653388-3653407 None:intergenic 20.0%
!! ATAATACCAATCAAATTTCA+GGG - chr8_4:3653609-3653628 None:intergenic 20.0%
!!! TGGAATATTGTTATTGTAAA+TGG + chr8_4:3653913-3653932 Msa1307730:intron 20.0%
!! TTTGCCTATTATTATTATGA+TGG + chr8_4:3653979-3653998 Msa1307730:intron 20.0%
!!! TAGAGTGAAATTTCTAATTT+TGG + chr8_4:3654106-3654125 Msa1307730:intron 20.0%
!!! AAAATTAAAATTGGGTCTTT+TGG + chr8_4:3654497-3654516 Msa1307730:three_prime_UTR 20.0%
!! ATTGCTTGGATATATATAAT+TGG + chr8_4:3654768-3654787 Msa1307730:three_prime_UTR 20.0%
!!! CAATAGTATTTTAAAAGAGA+AGG - chr8_4:3654805-3654824 None:intergenic 20.0%
!!! CATGATTAAGTTTTAATTGG+TGG + chr8_4:3653404-3653423 Msa1307730:intron 25.0%
! GATAATACCAATCAAATTTC+AGG - chr8_4:3653610-3653629 None:intergenic 25.0%
!! AATTAGGTTTTTCTATCTGT+TGG + chr8_4:3653666-3653685 Msa1307730:intron 25.0%
! TTGGAATGTCAAAATTACAT+AGG - chr8_4:3653776-3653795 None:intergenic 25.0%
! AACAATTTAAGGGACTAAAT+TGG + chr8_4:3653953-3653972 Msa1307730:intron 25.0%
! ATTTCACTCTAATCATGAAT+TGG - chr8_4:3654097-3654116 None:intergenic 25.0%
!! AGTTTTCCTCATATTGATAA+TGG + chr8_4:3654129-3654148 Msa1307730:intron 25.0%
! AAAACACCATTATCAATATG+AGG - chr8_4:3654138-3654157 None:intergenic 25.0%
! CTTCATATGTTACTGAAATT+TGG - chr8_4:3654180-3654199 None:intergenic 25.0%
! CAAATTTCAGTAACATATGA+AGG + chr8_4:3654178-3654197 Msa1307730:intron 25.0%
! TCTATCTGGACAATAATAAA+TGG - chr8_4:3654223-3654242 None:intergenic 25.0%
! GTTAAATCATCAATATCACT+TGG + chr8_4:3654252-3654271 Msa1307730:intron 25.0%
! GTTTAAACACAATTCTAAAC+AGG - chr8_4:3654548-3654567 None:intergenic 25.0%
! ATTGTGTTTAAACTCTTTGA+AGG + chr8_4:3654555-3654574 Msa1307730:three_prime_UTR 25.0%
! TGTTGAAGATCAATGAATAT+TGG + chr8_4:3654584-3654603 Msa1307730:three_prime_UTR 25.0%
! GTTGAAGATCAATGAATATT+GGG + chr8_4:3654585-3654604 Msa1307730:three_prime_UTR 25.0%
! TTGAAGATCAATGAATATTG+GGG + chr8_4:3654586-3654605 Msa1307730:three_prime_UTR 25.0%
!! TGCAATTTGCATCTAAAATT+TGG + chr8_4:3654639-3654658 Msa1307730:three_prime_UTR 25.0%
! TATATATCCAAGCAATCAAT+TGG - chr8_4:3654764-3654783 None:intergenic 25.0%
!! TTTAATTTCTGCTTAGTGTT+TGG + chr8_4:3654967-3654986 Msa1307730:three_prime_UTR 25.0%
!!! CATGATTAAGTTTTAATTGG+TGG + chr8_4:3653404-3653423 Msa1307730:intron 25.0%
! GATAATACCAATCAAATTTC+AGG - chr8_4:3653610-3653629 None:intergenic 25.0%
!! AATTAGGTTTTTCTATCTGT+TGG + chr8_4:3653666-3653685 Msa1307730:intron 25.0%
! TTGGAATGTCAAAATTACAT+AGG - chr8_4:3653776-3653795 None:intergenic 25.0%
! AACAATTTAAGGGACTAAAT+TGG + chr8_4:3653953-3653972 Msa1307730:intron 25.0%
! ATTTCACTCTAATCATGAAT+TGG - chr8_4:3654097-3654116 None:intergenic 25.0%
!! AGTTTTCCTCATATTGATAA+TGG + chr8_4:3654129-3654148 Msa1307730:intron 25.0%
! AAAACACCATTATCAATATG+AGG - chr8_4:3654138-3654157 None:intergenic 25.0%
! CTTCATATGTTACTGAAATT+TGG - chr8_4:3654180-3654199 None:intergenic 25.0%
! CAAATTTCAGTAACATATGA+AGG + chr8_4:3654178-3654197 Msa1307730:intron 25.0%
! TCTATCTGGACAATAATAAA+TGG - chr8_4:3654223-3654242 None:intergenic 25.0%
! GTTAAATCATCAATATCACT+TGG + chr8_4:3654252-3654271 Msa1307730:intron 25.0%
! GTTTAAACACAATTCTAAAC+AGG - chr8_4:3654548-3654567 None:intergenic 25.0%
! ATTGTGTTTAAACTCTTTGA+AGG + chr8_4:3654555-3654574 Msa1307730:three_prime_UTR 25.0%
! TGTTGAAGATCAATGAATAT+TGG + chr8_4:3654584-3654603 Msa1307730:three_prime_UTR 25.0%
! GTTGAAGATCAATGAATATT+GGG + chr8_4:3654585-3654604 Msa1307730:three_prime_UTR 25.0%
! TTGAAGATCAATGAATATTG+GGG + chr8_4:3654586-3654605 Msa1307730:three_prime_UTR 25.0%
!! TGCAATTTGCATCTAAAATT+TGG + chr8_4:3654639-3654658 Msa1307730:three_prime_UTR 25.0%
! TATATATCCAAGCAATCAAT+TGG - chr8_4:3654764-3654783 None:intergenic 25.0%
TTTGAAGGTCAGCAAATTAT+GGG + chr8_4:3653113-3653132 Msa1307730:CDS 30.0%
! TTGCAACTTTATCTGGTTTA+GGG + chr8_4:3653486-3653505 Msa1307730:intron 30.0%
!!! CTTGTTTTTGTGATTGTAGT+TGG + chr8_4:3653516-3653535 Msa1307730:intron 30.0%
TCAAATTTCAGGGATCAAAT+TGG - chr8_4:3653599-3653618 None:intergenic 30.0%
TTTGATCCCTGAAATTTGAT+TGG + chr8_4:3653600-3653619 Msa1307730:intron 30.0%
TGTCATGTTAGAAATGCATT+AGG - chr8_4:3653813-3653832 None:intergenic 30.0%
TTCTAACATGACAAACGTAT+CGG + chr8_4:3653820-3653839 Msa1307730:intron 30.0%
AGTTAGATTCATCTCTTTGT+TGG - chr8_4:3653866-3653885 None:intergenic 30.0%
! TGAAATTTGGTCTTTCTCTA+TGG - chr8_4:3654167-3654186 None:intergenic 30.0%
ATTTCAGTAACATATGAAGG+CGG + chr8_4:3654181-3654200 Msa1307730:intron 30.0%
TTAACAATCACAGTTCTATC+TGG - chr8_4:3654237-3654256 None:intergenic 30.0%
TGAAGATCAATGAATATTGG+GGG + chr8_4:3654587-3654606 Msa1307730:three_prime_UTR 30.0%
AGATGCAAATTGCATGAATA+TGG - chr8_4:3654633-3654652 None:intergenic 30.0%
! ATTTGATTTCATTGCTGAGT+TGG + chr8_4:3654699-3654718 Msa1307730:three_prime_UTR 30.0%
! ATACAAGACTTGCTTTAGAT+TGG - chr8_4:3654899-3654918 None:intergenic 30.0%
TTTGAAGGTCAGCAAATTAT+GGG + chr8_4:3653113-3653132 Msa1307730:CDS 30.0%
! TTGCAACTTTATCTGGTTTA+GGG + chr8_4:3653486-3653505 Msa1307730:intron 30.0%
!!! CTTGTTTTTGTGATTGTAGT+TGG + chr8_4:3653516-3653535 Msa1307730:intron 30.0%
TCAAATTTCAGGGATCAAAT+TGG - chr8_4:3653599-3653618 None:intergenic 30.0%
TTTGATCCCTGAAATTTGAT+TGG + chr8_4:3653600-3653619 Msa1307730:intron 30.0%
TGTCATGTTAGAAATGCATT+AGG - chr8_4:3653813-3653832 None:intergenic 30.0%
TTCTAACATGACAAACGTAT+CGG + chr8_4:3653820-3653839 Msa1307730:intron 30.0%
AGTTAGATTCATCTCTTTGT+TGG - chr8_4:3653866-3653885 None:intergenic 30.0%
! TGAAATTTGGTCTTTCTCTA+TGG - chr8_4:3654167-3654186 None:intergenic 30.0%
ATTTCAGTAACATATGAAGG+CGG + chr8_4:3654181-3654200 Msa1307730:intron 30.0%
TTAACAATCACAGTTCTATC+TGG - chr8_4:3654237-3654256 None:intergenic 30.0%
TGAAGATCAATGAATATTGG+GGG + chr8_4:3654587-3654606 Msa1307730:three_prime_UTR 30.0%
AGATGCAAATTGCATGAATA+TGG - chr8_4:3654633-3654652 None:intergenic 30.0%
! ATTTGATTTCATTGCTGAGT+TGG + chr8_4:3654699-3654718 Msa1307730:three_prime_UTR 30.0%
! ATACAAGACTTGCTTTAGAT+TGG - chr8_4:3654899-3654918 None:intergenic 30.0%
GAGCTTATTTATAAGAAGCC+AGG + chr8_4:3652909-3652928 Msa1307730:five_prime_UTR 35.0%
!! CATAATTTGCTGACCTTCAA+AGG - chr8_4:3653114-3653133 None:intergenic 35.0%
! CTTTGAAGGTCAGCAAATTA+TGG + chr8_4:3653112-3653131 Msa1307730:CDS 35.0%
ATTGACCTGACTGAATTTGA+GGG - chr8_4:3653288-3653307 None:intergenic 35.0%
AATTGACCTGACTGAATTTG+AGG - chr8_4:3653289-3653308 None:intergenic 35.0%
! GTTGCAACTTTATCTGGTTT+AGG + chr8_4:3653485-3653504 Msa1307730:intron 35.0%
!! GTTAATAGTTTTGCTGTCGT+AGG + chr8_4:3653551-3653570 Msa1307730:intron 35.0%
CTGTCGTAGGATTTCAATAT+TGG + chr8_4:3653564-3653583 Msa1307730:intron 35.0%
TCATTGTTCATTTGCATGCT+TGG + chr8_4:3653718-3653737 Msa1307730:intron 35.0%
TTAGGAGCTAAGTGTGTATT+TGG - chr8_4:3653795-3653814 None:intergenic 35.0%
TGAACTAAATTCTCGTTCTG+TGG + chr8_4:3654042-3654061 Msa1307730:intron 35.0%
TAAATTCTCGTTCTGTGGTT+GGG + chr8_4:3654047-3654066 Msa1307730:intron 35.0%
TCACTCTAATCATGAATTGG+TGG - chr8_4:3654094-3654113 None:intergenic 35.0%
TGAGAAATAAGAGCAGGATT+AGG - chr8_4:3654306-3654325 None:intergenic 35.0%
CAAGATTGAGAAATAAGAGC+AGG - chr8_4:3654312-3654331 None:intergenic 35.0%
AACACATAATAGCTTCCTTG+CGG - chr8_4:3654389-3654408 None:intergenic 35.0%
TTCTTCACGCATAGTTCAAA+CGG - chr8_4:3654424-3654443 None:intergenic 35.0%
ATCCAAGCAATCAATTGGAA+TGG - chr8_4:3654759-3654778 None:intergenic 35.0%
CAGCGACAATTCAAATTTAG+AGG + chr8_4:3654837-3654856 Msa1307730:three_prime_UTR 35.0%
ATTATTGCAGTGTCATGCTT+AGG + chr8_4:3654936-3654955 Msa1307730:three_prime_UTR 35.0%
GAGCTTATTTATAAGAAGCC+AGG + chr8_4:3652909-3652928 Msa1307730:five_prime_UTR 35.0%
!! CATAATTTGCTGACCTTCAA+AGG - chr8_4:3653114-3653133 None:intergenic 35.0%
! CTTTGAAGGTCAGCAAATTA+TGG + chr8_4:3653112-3653131 Msa1307730:CDS 35.0%
ATTGACCTGACTGAATTTGA+GGG - chr8_4:3653288-3653307 None:intergenic 35.0%
AATTGACCTGACTGAATTTG+AGG - chr8_4:3653289-3653308 None:intergenic 35.0%
! GTTGCAACTTTATCTGGTTT+AGG + chr8_4:3653485-3653504 Msa1307730:intron 35.0%
!! GTTAATAGTTTTGCTGTCGT+AGG + chr8_4:3653551-3653570 Msa1307730:intron 35.0%
CTGTCGTAGGATTTCAATAT+TGG + chr8_4:3653564-3653583 Msa1307730:intron 35.0%
TCATTGTTCATTTGCATGCT+TGG + chr8_4:3653718-3653737 Msa1307730:intron 35.0%
TTAGGAGCTAAGTGTGTATT+TGG - chr8_4:3653795-3653814 None:intergenic 35.0%
TGAACTAAATTCTCGTTCTG+TGG + chr8_4:3654042-3654061 Msa1307730:intron 35.0%
TAAATTCTCGTTCTGTGGTT+GGG + chr8_4:3654047-3654066 Msa1307730:intron 35.0%
TCACTCTAATCATGAATTGG+TGG - chr8_4:3654094-3654113 None:intergenic 35.0%
TGAGAAATAAGAGCAGGATT+AGG - chr8_4:3654306-3654325 None:intergenic 35.0%
CAAGATTGAGAAATAAGAGC+AGG - chr8_4:3654312-3654331 None:intergenic 35.0%
AACACATAATAGCTTCCTTG+CGG - chr8_4:3654389-3654408 None:intergenic 35.0%
TTCTTCACGCATAGTTCAAA+CGG - chr8_4:3654424-3654443 None:intergenic 35.0%
ATCCAAGCAATCAATTGGAA+TGG - chr8_4:3654759-3654778 None:intergenic 35.0%
CAGCGACAATTCAAATTTAG+AGG + chr8_4:3654837-3654856 Msa1307730:three_prime_UTR 35.0%
ATTATTGCAGTGTCATGCTT+AGG + chr8_4:3654936-3654955 Msa1307730:three_prime_UTR 35.0%
ATTTATAAGAAGCCAGGCCA+AGG + chr8_4:3652915-3652934 Msa1307730:five_prime_UTR 40.0%
CAGAGAAAGAGATCGTGTTA+CGG + chr8_4:3652969-3652988 Msa1307730:five_prime_UTR 40.0%
AATGCCTTTGACAATGCGTT+TGG - chr8_4:3653014-3653033 None:intergenic 40.0%
TCTAGCGTTGCAACTTTATC+TGG + chr8_4:3653479-3653498 Msa1307730:intron 40.0%
CATTTGCATGCTTGGATTTG+CGG + chr8_4:3653726-3653745 Msa1307730:intron 40.0%
CTAAATTCTCGTTCTGTGGT+TGG + chr8_4:3654046-3654065 Msa1307730:intron 40.0%
GGGGGTTGAAAACTTTATAC+TGG + chr8_4:3654605-3654624 Msa1307730:three_prime_UTR 40.0%
!! ACTTTGTGCTGGCTACATAA+CGG - chr8_4:3654729-3654748 None:intergenic 40.0%
AGCCATTCCAATTGATTGCT+TGG + chr8_4:3654754-3654773 Msa1307730:three_prime_UTR 40.0%
ATTTATAAGAAGCCAGGCCA+AGG + chr8_4:3652915-3652934 Msa1307730:five_prime_UTR 40.0%
CAGAGAAAGAGATCGTGTTA+CGG + chr8_4:3652969-3652988 Msa1307730:five_prime_UTR 40.0%
AATGCCTTTGACAATGCGTT+TGG - chr8_4:3653014-3653033 None:intergenic 40.0%
TCTAGCGTTGCAACTTTATC+TGG + chr8_4:3653479-3653498 Msa1307730:intron 40.0%
CATTTGCATGCTTGGATTTG+CGG + chr8_4:3653726-3653745 Msa1307730:intron 40.0%
CTAAATTCTCGTTCTGTGGT+TGG + chr8_4:3654046-3654065 Msa1307730:intron 40.0%
GGGGGTTGAAAACTTTATAC+TGG + chr8_4:3654605-3654624 Msa1307730:three_prime_UTR 40.0%
!! ACTTTGTGCTGGCTACATAA+CGG - chr8_4:3654729-3654748 None:intergenic 40.0%
AGCCATTCCAATTGATTGCT+TGG + chr8_4:3654754-3654773 Msa1307730:three_prime_UTR 40.0%
AGTCTCTCTATCTCACTCCT+TGG - chr8_4:3652935-3652954 None:intergenic 45.0%
AGAAAGAGATCGTGTTACGG+CGG + chr8_4:3652972-3652991 Msa1307730:five_prime_UTR 45.0%
GGATCCAAACGCATTGTCAA+AGG + chr8_4:3653007-3653026 Msa1307730:CDS 45.0%
AGAACCATCTTGGTAGAGAG+TGG - chr8_4:3653084-3653103 None:intergenic 45.0%
AGGTGAGCATAGAACCATCT+TGG - chr8_4:3653094-3653113 None:intergenic 45.0%
GGTTCTATGCTCACCTTTGA+AGG + chr8_4:3653098-3653117 Msa1307730:CDS 45.0%
TCAATTCCCTCTCTCTCTCT+AGG + chr8_4:3653303-3653322 Msa1307730:intron 45.0%
CAATTCCCTCTCTCTCTCTA+GGG + chr8_4:3653304-3653323 Msa1307730:intron 45.0%
GAAAACCCTAGAGAGAGAGA+GGG - chr8_4:3653312-3653331 None:intergenic 45.0%
AGAAAACCCTAGAGAGAGAG+AGG - chr8_4:3653313-3653332 None:intergenic 45.0%
TGGTTGGGCAATGGGAATTA+GGG + chr8_4:3654062-3654081 Msa1307730:intron 45.0%
!! CTTGCGGTGTTGGTATCAAA+TGG - chr8_4:3654373-3654392 None:intergenic 45.0%
CATTTGATACCAACACCGCA+AGG + chr8_4:3654371-3654390 Msa1307730:CDS 45.0%
TAATAGCTTCCTTGCGGTGT+TGG - chr8_4:3654383-3654402 None:intergenic 45.0%
AGTCTCTCTATCTCACTCCT+TGG - chr8_4:3652935-3652954 None:intergenic 45.0%
AGAAAGAGATCGTGTTACGG+CGG + chr8_4:3652972-3652991 Msa1307730:five_prime_UTR 45.0%
GGATCCAAACGCATTGTCAA+AGG + chr8_4:3653007-3653026 Msa1307730:CDS 45.0%
AGAACCATCTTGGTAGAGAG+TGG - chr8_4:3653084-3653103 None:intergenic 45.0%
AGGTGAGCATAGAACCATCT+TGG - chr8_4:3653094-3653113 None:intergenic 45.0%
GGTTCTATGCTCACCTTTGA+AGG + chr8_4:3653098-3653117 Msa1307730:CDS 45.0%
TCAATTCCCTCTCTCTCTCT+AGG + chr8_4:3653303-3653322 Msa1307730:intron 45.0%
CAATTCCCTCTCTCTCTCTA+GGG + chr8_4:3653304-3653323 Msa1307730:intron 45.0%
GAAAACCCTAGAGAGAGAGA+GGG - chr8_4:3653312-3653331 None:intergenic 45.0%
AGAAAACCCTAGAGAGAGAG+AGG - chr8_4:3653313-3653332 None:intergenic 45.0%
TGGTTGGGCAATGGGAATTA+GGG + chr8_4:3654062-3654081 Msa1307730:intron 45.0%
!! CTTGCGGTGTTGGTATCAAA+TGG - chr8_4:3654373-3654392 None:intergenic 45.0%
CATTTGATACCAACACCGCA+AGG + chr8_4:3654371-3654390 Msa1307730:CDS 45.0%
TAATAGCTTCCTTGCGGTGT+TGG - chr8_4:3654383-3654402 None:intergenic 45.0%
!! CGCATTGTCAAAGGCATTCG+TGG + chr8_4:3653016-3653035 Msa1307730:CDS 50.0%
TGGTAGAGAGTGGCGAGATT+GGG - chr8_4:3653074-3653093 None:intergenic 50.0%
TTGGTAGAGAGTGGCGAGAT+TGG - chr8_4:3653075-3653094 None:intergenic 50.0%
AGTGATGGCACTGCTGGAAA+GGG - chr8_4:3653172-3653191 None:intergenic 50.0%
TTGACAATCGACGGTGGTGA+TGG - chr8_4:3653195-3653214 None:intergenic 50.0%
AGAAGGTTGACAATCGACGG+TGG - chr8_4:3653201-3653220 None:intergenic 50.0%
! ACCGTCGATTGTCAACCTTC+TGG + chr8_4:3653200-3653219 Msa1307730:CDS 50.0%
GCCAGAAGGTTGACAATCGA+CGG - chr8_4:3653204-3653223 None:intergenic 50.0%
TGTCAACCTTCTGGCGCTAA+CGG + chr8_4:3653209-3653228 Msa1307730:CDS 50.0%
GGCATGCTTGTCTTTGTCAG+TGG + chr8_4:3653233-3653252 Msa1307730:CDS 50.0%
ACACGCCCTCAAATTCAGTC+AGG + chr8_4:3653280-3653299 Msa1307730:CDS 50.0%
GCTTGGATTTGCGGTGAGTT+TGG + chr8_4:3653735-3653754 Msa1307730:intron 50.0%
TCGTTCTGTGGTTGGGCAAT+GGG + chr8_4:3654054-3654073 Msa1307730:intron 50.0%
GTGGTTGGGCAATGGGAATT+AGG + chr8_4:3654061-3654080 Msa1307730:intron 50.0%
ATGGCTAGCACACTTTGTGC+TGG - chr8_4:3654740-3654759 None:intergenic 50.0%
!! CGCATTGTCAAAGGCATTCG+TGG + chr8_4:3653016-3653035 Msa1307730:CDS 50.0%
TGGTAGAGAGTGGCGAGATT+GGG - chr8_4:3653074-3653093 None:intergenic 50.0%
TTGGTAGAGAGTGGCGAGAT+TGG - chr8_4:3653075-3653094 None:intergenic 50.0%
AGTGATGGCACTGCTGGAAA+GGG - chr8_4:3653172-3653191 None:intergenic 50.0%
TTGACAATCGACGGTGGTGA+TGG - chr8_4:3653195-3653214 None:intergenic 50.0%
AGAAGGTTGACAATCGACGG+TGG - chr8_4:3653201-3653220 None:intergenic 50.0%
! ACCGTCGATTGTCAACCTTC+TGG + chr8_4:3653200-3653219 Msa1307730:CDS 50.0%
GCCAGAAGGTTGACAATCGA+CGG - chr8_4:3653204-3653223 None:intergenic 50.0%
TGTCAACCTTCTGGCGCTAA+CGG + chr8_4:3653209-3653228 Msa1307730:CDS 50.0%
GGCATGCTTGTCTTTGTCAG+TGG + chr8_4:3653233-3653252 Msa1307730:CDS 50.0%
ACACGCCCTCAAATTCAGTC+AGG + chr8_4:3653280-3653299 Msa1307730:CDS 50.0%
GCTTGGATTTGCGGTGAGTT+TGG + chr8_4:3653735-3653754 Msa1307730:intron 50.0%
TCGTTCTGTGGTTGGGCAAT+GGG + chr8_4:3654054-3654073 Msa1307730:intron 50.0%
GTGGTTGGGCAATGGGAATT+AGG + chr8_4:3654061-3654080 Msa1307730:intron 50.0%
ATGGCTAGCACACTTTGTGC+TGG - chr8_4:3654740-3654759 None:intergenic 50.0%
CTCTATCTCACTCCTTGGCC+TGG - chr8_4:3652930-3652949 None:intergenic 55.0%
!! ATTGGGACGGTTGGTGTCGA+AGG - chr8_4:3653057-3653076 None:intergenic 55.0%
AGAGAGTGGCGAGATTGGGA+CGG - chr8_4:3653070-3653089 None:intergenic 55.0%
CTCGCCACTCTCTACCAAGA+TGG + chr8_4:3653077-3653096 Msa1307730:CDS 55.0%
GAGTGATGGCACTGCTGGAA+AGG - chr8_4:3653173-3653192 None:intergenic 55.0%
TGATGGAGTGATGGCACTGC+TGG - chr8_4:3653178-3653197 None:intergenic 55.0%
AGTGGTAATCTCCAGCTCGC+CGG + chr8_4:3653251-3653270 Msa1307730:CDS 55.0%
! TTTGAGGGCGTGTTGTTCGC+CGG - chr8_4:3653273-3653292 None:intergenic 55.0%
CTCGTTCTGTGGTTGGGCAA+TGG + chr8_4:3654053-3654072 Msa1307730:intron 55.0%
CTCTATCTCACTCCTTGGCC+TGG - chr8_4:3652930-3652949 None:intergenic 55.0%
!! ATTGGGACGGTTGGTGTCGA+AGG - chr8_4:3653057-3653076 None:intergenic 55.0%
AGAGAGTGGCGAGATTGGGA+CGG - chr8_4:3653070-3653089 None:intergenic 55.0%
CTCGCCACTCTCTACCAAGA+TGG + chr8_4:3653077-3653096 Msa1307730:CDS 55.0%
GAGTGATGGCACTGCTGGAA+AGG - chr8_4:3653173-3653192 None:intergenic 55.0%
TGATGGAGTGATGGCACTGC+TGG - chr8_4:3653178-3653197 None:intergenic 55.0%
AGTGGTAATCTCCAGCTCGC+CGG + chr8_4:3653251-3653270 Msa1307730:CDS 55.0%
! TTTGAGGGCGTGTTGTTCGC+CGG - chr8_4:3653273-3653292 None:intergenic 55.0%
CTCGTTCTGTGGTTGGGCAA+TGG + chr8_4:3654053-3654072 Msa1307730:intron 55.0%
TTACGGCGGCGTAAGAGCGA+TGG + chr8_4:3652986-3653005 Msa1307730:exon 60.0%
AGTGGCGAGATTGGGACGGT+TGG - chr8_4:3653066-3653085 None:intergenic 60.0%
!! CGACGGTGGTGATGGAGTGA+TGG - chr8_4:3653187-3653206 None:intergenic 60.0%
CAACCTTCTGGCGCTAACGG+CGG + chr8_4:3653212-3653231 Msa1307730:CDS 60.0%
TTACGGCGGCGTAAGAGCGA+TGG + chr8_4:3652986-3653005 Msa1307730:exon 60.0%
AGTGGCGAGATTGGGACGGT+TGG - chr8_4:3653066-3653085 None:intergenic 60.0%
!! CGACGGTGGTGATGGAGTGA+TGG - chr8_4:3653187-3653206 None:intergenic 60.0%
CAACCTTCTGGCGCTAACGG+CGG + chr8_4:3653212-3653231 Msa1307730:CDS 60.0%
!! GGGACGGTTGGTGTCGAAGG+TGG - chr8_4:3653054-3653073 None:intergenic 65.0%
ATGCCGCCGTTAGCGCCAGA+AGG - chr8_4:3653218-3653237 None:intergenic 65.0%
!! GGGACGGTTGGTGTCGAAGG+TGG - chr8_4:3653054-3653073 None:intergenic 65.0%
ATGCCGCCGTTAGCGCCAGA+AGG - chr8_4:3653218-3653237 None:intergenic 65.0%
! CGTGTTGTTCGCCGGCGAGC+TGG - chr8_4:3653265-3653284 None:intergenic 70.0%
! CGTGTTGTTCGCCGGCGAGC+TGG - chr8_4:3653265-3653284 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 3652831 3654988 3652831 ID=Msa1307730;Name=Msa1307730
chr8_4 mRNA 3652831 3654988 3652831 ID=Msa1307730-mRNA-1;Parent=Msa1307730;Name=Msa1307730-mRNA-1;_AED=0.00;_eAED=0.00;_QI=174|1|1|1|1|1|2|549|123
chr8_4 exon 3652831 3653301 3652831 ID=Msa1307730-mRNA-1:exon:1065;Parent=Msa1307730-mRNA-1
chr8_4 exon 3654365 3654988 3654365 ID=Msa1307730-mRNA-1:exon:1066;Parent=Msa1307730-mRNA-1
chr8_4 five_prime_UTR 3652831 3653004 3652831 ID=Msa1307730-mRNA-1:five_prime_utr;Parent=Msa1307730-mRNA-1
chr8_4 CDS 3653005 3653301 3653005 ID=Msa1307730-mRNA-1:cds;Parent=Msa1307730-mRNA-1
chr8_4 CDS 3654365 3654439 3654365 ID=Msa1307730-mRNA-1:cds;Parent=Msa1307730-mRNA-1
chr8_4 three_prime_UTR 3654440 3654988 3654440 ID=Msa1307730-mRNA-1:three_prime_utr;Parent=Msa1307730-mRNA-1
Gene Sequence

>Msa1307730

ATGGATCCAAACGCATTGTCAAAGGCATTCGTGGAACACTACTACACCACCTTCGACACCAACCGTCCCAATCTCGCCACTCTCTACCAAGATGGTTCTATGCTCACCTTTGAAGGTCAGCAAATTATGGGATCTCAGAATATCGTCGCTAAACTCACTTCTCTCCCTTTCCAGCAGTGCCATCACTCCATCACCACCGTCGATTGTCAACCTTCTGGCGCTAACGGCGGCATGCTTGTCTTTGTCAGTGGTAATCTCCAGCTCGCCGGCGAACAACACGCCCTCAAATTCAGTCAGATGTTCCATTTGATACCAACACCGCAAGGAAGCTATTATGTGTTGAATGACATATTCCGTTTGAACTATGCGTGA
Protein sequence

>Msa1307730

MDPNALSKAFVEHYYTTFDTNRPNLATLYQDGSMLTFEGQQIMGSQNIVAKLTSLPFQQCHHSITTVDCQPSGANGGMLVFVSGNLQLAGEQHALKFSQMFHLIPTPQGSYYVLNDIFRLNYA*