AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1377200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1377200 MtrunA17_Chr2g0323021 94.298 228 13 0 1 228 1 228 7.50e-149 413
Msa1377200 MtrunA17_Chr6g0481491 45.133 113 61 1 10 122 13 124 1.81e-34 122
Msa1377200 MtrunA17_Chr8g0335571 44.828 116 59 3 11 122 8 122 5.23e-30 110
Msa1377200 MtrunA17_Chr1g0196801 46.392 97 51 1 22 118 20 115 2.15e-29 108
Msa1377200 MtrunA17_Chr8g0335531 45.690 116 58 3 11 122 8 122 2.36e-29 108
Msa1377200 MtrunA17_Chr3g0132431 46.457 127 56 5 12 136 12 128 3.11e-28 105
Msa1377200 MtrunA17_Chr6g0459441 47.059 102 52 2 18 118 19 119 1.06e-27 103
Msa1377200 MtrunA17_Chr3g0132441 45.918 98 52 1 21 118 22 118 2.51e-25 98.2
Msa1377200 MtrunA17_Chr3g0132701 43.802 121 64 4 1 118 1 120 5.12e-25 98.2
Msa1377200 MtrunA17_Chr3g0132511 43.802 121 64 4 1 118 1 120 1.11e-24 97.4
Msa1377200 MtrunA17_Chr8g0380901 40.870 115 63 4 6 119 9 119 1.47e-24 94.0
Msa1377200 MtrunA17_Chr1g0205701 38.211 123 70 3 6 125 8 127 2.01e-24 95.5
Msa1377200 MtrunA17_Chr5g0394321 35.057 174 92 7 1 168 1 159 2.86e-24 95.1
Msa1377200 MtrunA17_Chr2g0289741 36.486 148 80 6 1 141 4 144 5.19e-24 94.4
Msa1377200 MtrunA17_Chr1g0150131 37.313 134 78 3 8 136 9 141 5.72e-24 94.4
Msa1377200 MtrunA17_Chr3g0132411 42.857 119 61 3 12 129 51 163 5.74e-22 89.0
Msa1377200 MtrunA17_Chr3g0136871 33.548 155 83 4 22 171 24 163 8.27e-21 85.9
Msa1377200 MtrunA17_Chr8g0360371 32.836 134 86 3 4 134 3 135 8.67e-21 88.6
Msa1377200 MtrunA17_Chr4g0064041 36.923 130 75 4 6 129 9 137 3.11e-20 87.8
Msa1377200 MtrunA17_Chr4g0064041 44.565 92 46 2 33 119 198 289 2.45e-19 85.5
Msa1377200 MtrunA17_Chr6g0453921 36.913 149 84 7 6 147 18 163 5.64e-20 84.0
Msa1377200 MtrunA17_Chr7g0253701 36.975 119 71 4 6 122 8 124 9.17e-20 82.8
Msa1377200 MtrunA17_Chr7g0253671 36.975 119 71 4 6 122 8 124 9.17e-20 82.8
Msa1377200 MtrunA17_Chr2g0324711 37.600 125 74 3 1 122 1 124 1.93e-19 85.5
Msa1377200 MtrunA17_Chr2g0301431 37.607 117 65 5 7 119 11 123 5.94e-19 79.7
Msa1377200 MtrunA17_Chr2g0306461 36.207 116 70 2 6 118 9 123 1.42e-18 80.5
Msa1377200 MtrunA17_Chr2g0331181 37.615 109 61 3 27 130 29 135 1.96e-18 80.1
Msa1377200 MtrunA17_Chr1g0177341 34.746 118 73 4 6 121 7 122 4.16e-18 78.2
Msa1377200 MtrunA17_Chr1g0177331 34.746 118 73 4 6 121 7 122 4.16e-18 78.2
Msa1377200 MtrunA17_Chr5g0440711 35.433 127 77 4 5 128 9 133 1.39e-17 76.6
Msa1377200 MtrunA17_Chr7g0253681 33.613 119 76 3 6 122 8 125 1.62e-17 77.0
Msa1377200 MtrunA17_Chr6g0453801 33.540 161 99 5 6 163 16 171 5.50e-17 75.9
Msa1377200 MtrunA17_Chr4g0040291 31.169 154 86 6 27 177 27 163 5.85e-17 75.9
Msa1377200 MtrunA17_Chr4g0073921 35.659 129 74 4 6 128 9 134 8.07e-17 77.0
Msa1377200 MtrunA17_Chr2g0324721 31.868 182 107 6 4 180 6 175 9.07e-17 75.9
Msa1377200 MtrunA17_Chr7g0253731 34.746 118 73 4 7 122 8 123 9.13e-17 74.7
Msa1377200 MtrunA17_Chr4g0041811 34.783 115 69 3 13 122 20 133 3.20e-16 74.7
Msa1377200 MtrunA17_Chr8g0387804 35.833 120 68 4 3 118 11 125 5.30e-16 72.0
Msa1377200 MtrunA17_Chr8g0385021 30.151 199 110 7 4 180 3 194 7.54e-16 74.7
Msa1377200 MtrunA17_Chr6g0458331 36.559 93 58 1 43 135 59 150 8.56e-16 72.4
Msa1377200 MtrunA17_Chr8g0385031 30.000 220 114 8 4 195 3 210 1.36e-15 74.3
Msa1377200 MtrunA17_Chr3g0125791 40.000 100 55 4 20 118 28 123 1.73e-15 70.5
Msa1377200 MtrunA17_Chr7g0253631 35.897 117 68 3 9 119 5 120 2.26e-15 72.8
Msa1377200 MtrunA17_Chr4g0062341 31.579 152 92 5 6 147 13 162 2.52e-15 71.6
Msa1377200 MtrunA17_Chr4g0062311 31.579 152 92 5 6 147 13 162 2.52e-15 71.6
Msa1377200 MtrunA17_Chr3g0125761 34.454 119 69 6 5 119 11 124 2.71e-15 70.1
Msa1377200 MtrunA17_Chr4g0039721 31.884 138 85 4 6 136 13 148 2.90e-15 71.2
Msa1377200 MtrunA17_Chr4g0073901 30.435 138 85 4 6 137 9 141 3.68e-15 70.9
Msa1377200 MtrunA17_Chr3g0144921 32.520 123 80 3 25 145 30 151 5.61e-15 70.1
Msa1377200 MtrunA17_Chr1g0188981 38.961 77 46 1 43 118 51 127 5.86e-15 70.9
Msa1377200 MtrunA17_Chr7g0253641 34.615 104 66 2 19 121 20 122 7.46e-15 71.2
Msa1377200 MtrunA17_Chr2g0317231 33.333 129 78 4 1 124 1 126 7.97e-15 73.2
Msa1377200 MtrunA17_Chr4g0064031 33.582 134 78 5 14 137 17 149 1.07e-14 69.3
Msa1377200 MtrunA17_Chr6g0453791 38.760 129 67 8 25 148 31 152 1.81e-14 68.9
Msa1377200 MtrunA17_Chr5g0440804 34.821 112 68 4 20 128 24 133 3.07e-14 68.2
Msa1377200 MtrunA17_Chr6g0483981 33.333 162 92 5 42 199 47 196 3.40e-14 71.2
Msa1377200 MtrunA17_Chr4g0033131 34.091 132 74 5 6 130 9 134 1.18e-13 66.6
Msa1377200 MtrunA17_Chr4g0033531 34.091 132 74 5 6 130 9 134 1.18e-13 66.6
Msa1377200 MtrunA17_Chr7g0253761 33.981 103 62 4 19 118 20 119 1.17e-12 63.9
Msa1377200 MtrunA17_Chr2g0319611 37.037 108 60 5 22 122 23 129 1.41e-12 65.5
Msa1377200 MtrunA17_Chr7g0256441 29.221 154 74 4 22 171 24 146 3.37e-12 62.4
Msa1377200 MtrunA17_Chr8g0385041 35.714 154 79 9 27 169 27 171 3.61e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1377200 AT2G32300.1 48.507 134 65 4 1 131 1 133 2.37e-42 144
Msa1377200 AT2G32300.2 48.507 134 65 4 1 131 31 163 1.18e-41 143
Msa1377200 AT3G27200.1 38.136 118 70 3 6 121 6 122 7.04e-29 107
Msa1377200 AT3G60270.1 48.936 94 48 0 29 122 29 122 2.06e-27 103
Msa1377200 AT5G07475.1 35.849 159 94 4 4 159 9 162 2.15e-27 103
Msa1377200 AT5G53870.2 34.737 190 106 6 4 177 10 197 1.33e-26 105
Msa1377200 AT5G53870.1 34.737 190 106 6 4 177 10 197 1.78e-26 105
Msa1377200 AT2G25060.1 36.232 138 83 2 15 147 21 158 9.03e-25 96.7
Msa1377200 AT2G31050.1 39.062 128 70 3 12 131 10 137 5.05e-24 95.5
Msa1377200 AT2G26720.1 39.640 111 66 1 12 121 16 126 1.29e-23 94.4
Msa1377200 AT4G30590.1 35.115 131 75 4 1 122 1 130 3.77e-22 90.1
Msa1377200 AT4G31840.1 35.772 123 73 3 27 144 29 150 3.57e-21 87.4
Msa1377200 AT4G27520.1 38.686 137 75 5 4 135 13 145 5.49e-21 90.1
Msa1377200 AT3G60280.1 42.222 90 52 0 33 122 31 120 1.16e-20 87.0
Msa1377200 AT1G72230.1 42.353 85 49 0 34 118 31 115 1.80e-20 85.5
Msa1377200 AT5G57920.1 35.294 102 61 2 26 122 24 125 4.08e-20 84.7
Msa1377200 AT5G57920.3 35.294 102 61 2 26 122 56 157 6.59e-20 84.7
Msa1377200 AT5G57920.2 35.294 102 61 2 26 122 23 124 1.32e-19 83.2
Msa1377200 AT5G26330.1 29.670 182 103 6 6 184 5 164 3.33e-19 82.4
Msa1377200 AT2G44790.1 40.659 91 52 1 34 122 36 126 1.13e-18 81.3
Msa1377200 AT3G17675.1 36.207 116 71 3 6 119 12 126 2.02e-18 78.6
Msa1377200 AT4G12880.1 39.000 100 56 4 22 118 26 123 5.12e-18 77.8
Msa1377200 AT5G25090.1 35.780 109 65 2 15 118 16 124 5.27e-18 79.0
Msa1377200 AT2G23990.1 35.897 117 69 3 27 137 29 145 2.40e-17 77.8
Msa1377200 AT1G22480.1 45.882 85 46 0 34 118 27 111 6.91e-17 75.9
Msa1377200 AT3G20570.1 35.714 112 67 3 15 122 20 130 1.55e-16 75.5
Msa1377200 AT1G64640.1 36.842 95 57 2 43 134 53 147 1.89e-15 72.4
Msa1377200 AT1G48940.1 35.115 131 76 4 1 124 1 129 2.13e-15 72.0
Msa1377200 AT4G28365.1 32.143 140 88 4 3 136 4 142 3.13e-15 72.0
Msa1377200 AT4G32490.1 28.144 167 118 2 3 167 9 175 3.10e-13 67.0
Msa1377200 AT2G23990.2 34.884 129 55 6 27 130 29 153 4.86e-13 66.6
Msa1377200 AT3G18590.1 31.452 124 72 4 9 121 6 127 7.62e-13 65.1
Msa1377200 AT5G14345.1 32.955 88 58 1 35 122 30 116 8.67e-13 64.3
Msa1377200 AT1G79800.1 31.250 96 65 1 43 138 56 150 1.48e-12 64.7
Msa1377200 AT5G20230.1 35.789 95 59 2 42 134 44 138 4.60e-12 63.2
Msa1377200 AT4G12880.2 38.889 72 40 3 49 118 16 85 9.80e-12 60.1
Msa1377200 AT2G02850.1 34.066 91 56 3 29 118 39 126 2.62e-11 59.7
Msa1377200 AT3G53330.1 31.356 118 72 4 29 142 191 303 4.96e-11 61.6

Find 63 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAAGGACTTGGCGCTTC+AGG 0.262695 tig0019874:+86169 None:intergenic
TGTGGCTATGCTAATCAAAT+TGG 0.271323 tig0019874:-86812 Msa1377200:CDS
CGATTTCAACCTTCATTCCT+TGG 0.319611 tig0019874:+86320 None:intergenic
TACTTCATTTGTGGAACAAT+TGG 0.325954 tig0019874:-86352 Msa1377200:CDS
CCATCATTGTAGGATTGAAT+TGG 0.330428 tig0019874:+86412 None:intergenic
CATCATGGTTTGGTGGATAC+TGG 0.344644 tig0019874:+86482 None:intergenic
GTGTTTATGACCTTCTTCTA+AGG 0.345543 tig0019874:-85983 None:intergenic
ATTGAATTGGATTTGTTTGC+TGG 0.369249 tig0019874:+86425 None:intergenic
TAAACTCAACACTACTGAAA+TGG 0.375504 tig0019874:+86012 None:intergenic
TTTGTATCCCACCCACCATT+TGG 0.382646 tig0019874:+86760 None:intergenic
GGCTTGTAGATTTCCTTTCT+CGG 0.389537 tig0019874:+86036 None:intergenic
TGTCTCCCACTGAAAATTGC+TGG 0.409535 tig0019874:+86710 None:intergenic
ACCATTCCTCTCACATCAAC+AGG 0.409662 tig0019874:-86382 Msa1377200:CDS
AACTCAGTGGATGGAATCAT+TGG 0.412975 tig0019874:+86115 None:intergenic
GGTTGTGGCACCATCATTGT+AGG 0.418651 tig0019874:+86402 None:intergenic
GGCAAAAGATACTTCATTTG+TGG 0.426964 tig0019874:-86361 Msa1377200:CDS
ATTGAAGGTGCTGCTGCTAC+TGG 0.429684 tig0019874:+86265 None:intergenic
CAAACTCAAACCTTCAATCA+TGG 0.439667 tig0019874:-86741 Msa1377200:CDS
CACATTGTTGGAGGACCAAA+TGG 0.441037 tig0019874:-86775 Msa1377200:CDS
TCGGCCTCATCGACCGAGAA+AGG 0.446703 tig0019874:-86049 Msa1377200:CDS
GCTGCTGCTACTGGCGAGGC+AGG 0.458894 tig0019874:+86274 None:intergenic
ACTTCAACCACATCATGGTT+TGG 0.472827 tig0019874:+86472 None:intergenic
TGTGTTTCAAACAAAGGACT+TGG 0.474320 tig0019874:+86160 None:intergenic
TCCTGTGAATGTTGTGCTAT+AGG 0.480835 tig0019874:+86076 None:intergenic
TCCTATAGCACAACATTCAC+AGG 0.486894 tig0019874:-86077 Msa1377200:CDS
GAGGGAATGATGGAAATCAT+TGG 0.490266 tig0019874:+86232 None:intergenic
CCAATTCAATCCTACAATGA+TGG 0.493235 tig0019874:-86412 Msa1377200:CDS
AATTGGATTTGTTTGCTGGC+AGG 0.504490 tig0019874:+86429 None:intergenic
ATGGCTACAAATCACATTGT+TGG 0.506134 tig0019874:-86787 Msa1377200:CDS
ATTGGAGAGTCTGCTATTGA+AGG 0.507517 tig0019874:+86250 None:intergenic
ACATTGCAGCCAAGGAATGA+AGG 0.511245 tig0019874:-86329 Msa1377200:CDS
GTCTCCCACTGAAAATTGCT+GGG 0.517061 tig0019874:+86711 None:intergenic
ACAATTGGACATTGCAGCCA+AGG 0.522512 tig0019874:-86337 Msa1377200:CDS
TTGTAACTTCAACCACATCA+TGG 0.532734 tig0019874:+86467 None:intergenic
TCCGCTTGTGTTTCAAACAA+AGG 0.536728 tig0019874:+86154 None:intergenic
GCCTGTTGATGTGAGAGGAA+TGG 0.541643 tig0019874:+86381 None:intergenic
AACAATGTGATTTGTAGCCA+TGG 0.542196 tig0019874:+86789 None:intergenic
TCCTTTGTTTGAAACACAAG+CGG 0.548392 tig0019874:-86155 Msa1377200:CDS
GGGAAGATGCCCATGATTGA+AGG 0.551803 tig0019874:+86731 None:intergenic
AAACTCAAACCTTCAATCAT+GGG 0.565295 tig0019874:-86740 Msa1377200:CDS
AGGTTCTGCAGAGGGAATGA+TGG 0.574123 tig0019874:+86222 None:intergenic
TTGGAGGACCAAATGGTGGG+TGG 0.577194 tig0019874:-86768 Msa1377200:CDS
GATGAAACAGTAGATTCTGC+AGG 0.577330 tig0019874:+86202 None:intergenic
ATTTCCTTTCTCGGTCGATG+AGG 0.581260 tig0019874:+86045 None:intergenic
GGAGCTAGAAACTCAGTGGA+TGG 0.587416 tig0019874:+86106 None:intergenic
TGTTGTGCTATAGGAGAGGA+AGG 0.587966 tig0019874:+86085 None:intergenic
ACAACATTCACAGGATGTAT+CGG 0.591172 tig0019874:-86068 Msa1377200:CDS
TGGAGGACCAAATGGTGGGT+GGG 0.594340 tig0019874:-86767 Msa1377200:CDS
TATGCTAATCAAATTGGCCA+TGG 0.594784 tig0019874:-86806 Msa1377200:CDS
AGGTGCTGCTGCTACTGGCG+AGG 0.595101 tig0019874:+86270 None:intergenic
AGTAGTGTTGAGTTTAGTAG+CGG 0.598482 tig0019874:-86005 Msa1377200:CDS
GCTACAAATCACATTGTTGG+AGG 0.598903 tig0019874:-86784 Msa1377200:CDS
GGAATCATTGGAGAGAGTGT+AGG 0.602725 tig0019874:+86127 None:intergenic
ACTCAACACTACTGAAATGG+AGG 0.602890 tig0019874:+86015 None:intergenic
GATTCTGCAGGTTCTGCAGA+GGG 0.602914 tig0019874:+86214 None:intergenic
TCAACCACATCATGGTTTGG+TGG 0.604647 tig0019874:+86475 None:intergenic
AGATTCTGCAGGTTCTGCAG+AGG 0.632775 tig0019874:+86213 None:intergenic
GGAAGGAGCTAGAAACTCAG+TGG 0.633216 tig0019874:+86102 None:intergenic
TGATGTGAGAGGAATGGTTG+TGG 0.649255 tig0019874:+86387 None:intergenic
ATTGTTGGAGGACCAAATGG+TGG 0.666142 tig0019874:-86772 Msa1377200:CDS
GTATCCACCAAACCATGATG+TGG 0.674142 tig0019874:-86479 Msa1377200:CDS
TTGTTGGAGGACCAAATGGT+GGG 0.681492 tig0019874:-86771 Msa1377200:CDS
TGAATGTTGTGCTATAGGAG+AGG 0.726891 tig0019874:+86081 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATAGTAAGCGCTTAATTA+AGG - tig0019874:86209-86228 Msa1377200:CDS 25.0%
!!! ATGAATTACTTAGCTTTGTT+TGG + tig0019874:86242-86261 None:intergenic 25.0%
! ATATAGTAAGCGCTTAATTA+AGG - tig0019874:86209-86228 Msa1377200:CDS 25.0%
!!! ATGAATTACTTAGCTTTGTT+TGG + tig0019874:86242-86261 None:intergenic 25.0%
!!! CATTCTAAGAGTATCTTTTG+TGG - tig0019874:86002-86021 Msa1377200:CDS 30.0%
AAACTCAAACCTTCAATCAT+GGG - tig0019874:86092-86111 Msa1377200:CDS 30.0%
! ATTGAATTGGATTTGTTTGC+TGG + tig0019874:86410-86429 None:intergenic 30.0%
TACTTCATTTGTGGAACAAT+TGG - tig0019874:86480-86499 Msa1377200:CDS 30.0%
TAAACTCAACACTACTGAAA+TGG + tig0019874:86823-86842 None:intergenic 30.0%
!!! CATTCTAAGAGTATCTTTTG+TGG - tig0019874:86002-86021 Msa1377200:CDS 30.0%
AAACTCAAACCTTCAATCAT+GGG - tig0019874:86092-86111 Msa1377200:CDS 30.0%
! ATTGAATTGGATTTGTTTGC+TGG + tig0019874:86410-86429 None:intergenic 30.0%
TACTTCATTTGTGGAACAAT+TGG - tig0019874:86480-86499 Msa1377200:CDS 30.0%
TAAACTCAACACTACTGAAA+TGG + tig0019874:86823-86842 None:intergenic 30.0%
TGTGGCTATGCTAATCAAAT+TGG - tig0019874:86020-86039 Msa1377200:CDS 35.0%
TATGCTAATCAAATTGGCCA+TGG - tig0019874:86026-86045 Msa1377200:CDS 35.0%
! AACAATGTGATTTGTAGCCA+TGG + tig0019874:86046-86065 None:intergenic 35.0%
ATGGCTACAAATCACATTGT+TGG - tig0019874:86045-86064 Msa1377200:CDS 35.0%
CAAACTCAAACCTTCAATCA+TGG - tig0019874:86091-86110 Msa1377200:CDS 35.0%
TTGTAACTTCAACCACATCA+TGG + tig0019874:86368-86387 None:intergenic 35.0%
CCATCATTGTAGGATTGAAT+TGG + tig0019874:86423-86442 None:intergenic 35.0%
CCAATTCAATCCTACAATGA+TGG - tig0019874:86420-86439 Msa1377200:CDS 35.0%
GGCAAAAGATACTTCATTTG+TGG - tig0019874:86471-86490 Msa1377200:CDS 35.0%
TGTGTTTCAAACAAAGGACT+TGG + tig0019874:86675-86694 None:intergenic 35.0%
! TCCTTTGTTTGAAACACAAG+CGG - tig0019874:86677-86696 Msa1377200:intron 35.0%
ACAACATTCACAGGATGTAT+CGG - tig0019874:86764-86783 Msa1377200:CDS 35.0%
! AGTAGTGTTGAGTTTAGTAG+CGG - tig0019874:86827-86846 Msa1377200:CDS 35.0%
TGTGGCTATGCTAATCAAAT+TGG - tig0019874:86020-86039 Msa1377200:CDS 35.0%
TATGCTAATCAAATTGGCCA+TGG - tig0019874:86026-86045 Msa1377200:CDS 35.0%
! AACAATGTGATTTGTAGCCA+TGG + tig0019874:86046-86065 None:intergenic 35.0%
ATGGCTACAAATCACATTGT+TGG - tig0019874:86045-86064 Msa1377200:CDS 35.0%
CAAACTCAAACCTTCAATCA+TGG - tig0019874:86091-86110 Msa1377200:CDS 35.0%
TTGTAACTTCAACCACATCA+TGG + tig0019874:86368-86387 None:intergenic 35.0%
CCATCATTGTAGGATTGAAT+TGG + tig0019874:86423-86442 None:intergenic 35.0%
CCAATTCAATCCTACAATGA+TGG - tig0019874:86420-86439 Msa1377200:CDS 35.0%
GGCAAAAGATACTTCATTTG+TGG - tig0019874:86471-86490 Msa1377200:CDS 35.0%
TGTGTTTCAAACAAAGGACT+TGG + tig0019874:86675-86694 None:intergenic 35.0%
! TCCTTTGTTTGAAACACAAG+CGG - tig0019874:86677-86696 Msa1377200:intron 35.0%
ACAACATTCACAGGATGTAT+CGG - tig0019874:86764-86783 Msa1377200:CDS 35.0%
! AGTAGTGTTGAGTTTAGTAG+CGG - tig0019874:86827-86846 Msa1377200:CDS 35.0%
GCTACAAATCACATTGTTGG+AGG - tig0019874:86048-86067 Msa1377200:CDS 40.0%
! ATCTTCCCAGCAATTTTCAG+TGG - tig0019874:86116-86135 Msa1377200:CDS 40.0%
! TCTTCCCAGCAATTTTCAGT+GGG - tig0019874:86117-86136 Msa1377200:CDS 40.0%
ACTTCAACCACATCATGGTT+TGG + tig0019874:86363-86382 None:intergenic 40.0%
! AATTGGATTTGTTTGCTGGC+AGG + tig0019874:86406-86425 None:intergenic 40.0%
CGATTTCAACCTTCATTCCT+TGG + tig0019874:86515-86534 None:intergenic 40.0%
ATTGGAGAGTCTGCTATTGA+AGG + tig0019874:86585-86604 None:intergenic 40.0%
! GAGGGAATGATGGAAATCAT+TGG + tig0019874:86603-86622 None:intergenic 40.0%
GATGAAACAGTAGATTCTGC+AGG + tig0019874:86633-86652 None:intergenic 40.0%
TCCGCTTGTGTTTCAAACAA+AGG + tig0019874:86681-86700 None:intergenic 40.0%
AACTCAGTGGATGGAATCAT+TGG + tig0019874:86720-86739 None:intergenic 40.0%
TGAATGTTGTGCTATAGGAG+AGG + tig0019874:86754-86773 None:intergenic 40.0%
TCCTATAGCACAACATTCAC+AGG - tig0019874:86755-86774 Msa1377200:CDS 40.0%
TCCTGTGAATGTTGTGCTAT+AGG + tig0019874:86759-86778 None:intergenic 40.0%
GGCTTGTAGATTTCCTTTCT+CGG + tig0019874:86799-86818 None:intergenic 40.0%
ACTCAACACTACTGAAATGG+AGG + tig0019874:86820-86839 None:intergenic 40.0%
GCTACAAATCACATTGTTGG+AGG - tig0019874:86048-86067 Msa1377200:CDS 40.0%
! ATCTTCCCAGCAATTTTCAG+TGG - tig0019874:86116-86135 Msa1377200:CDS 40.0%
! TCTTCCCAGCAATTTTCAGT+GGG - tig0019874:86117-86136 Msa1377200:CDS 40.0%
ACTTCAACCACATCATGGTT+TGG + tig0019874:86363-86382 None:intergenic 40.0%
! AATTGGATTTGTTTGCTGGC+AGG + tig0019874:86406-86425 None:intergenic 40.0%
CGATTTCAACCTTCATTCCT+TGG + tig0019874:86515-86534 None:intergenic 40.0%
ATTGGAGAGTCTGCTATTGA+AGG + tig0019874:86585-86604 None:intergenic 40.0%
! GAGGGAATGATGGAAATCAT+TGG + tig0019874:86603-86622 None:intergenic 40.0%
GATGAAACAGTAGATTCTGC+AGG + tig0019874:86633-86652 None:intergenic 40.0%
TCCGCTTGTGTTTCAAACAA+AGG + tig0019874:86681-86700 None:intergenic 40.0%
AACTCAGTGGATGGAATCAT+TGG + tig0019874:86720-86739 None:intergenic 40.0%
TGAATGTTGTGCTATAGGAG+AGG + tig0019874:86754-86773 None:intergenic 40.0%
TCCTATAGCACAACATTCAC+AGG - tig0019874:86755-86774 Msa1377200:CDS 40.0%
TCCTGTGAATGTTGTGCTAT+AGG + tig0019874:86759-86778 None:intergenic 40.0%
GGCTTGTAGATTTCCTTTCT+CGG + tig0019874:86799-86818 None:intergenic 40.0%
ACTCAACACTACTGAAATGG+AGG + tig0019874:86820-86839 None:intergenic 40.0%
CACATTGTTGGAGGACCAAA+TGG - tig0019874:86057-86076 Msa1377200:CDS 45.0%
ATTGTTGGAGGACCAAATGG+TGG - tig0019874:86060-86079 Msa1377200:CDS 45.0%
TTGTTGGAGGACCAAATGGT+GGG - tig0019874:86061-86080 Msa1377200:CDS 45.0%
TTTGTATCCCACCCACCATT+TGG + tig0019874:86075-86094 None:intergenic 45.0%
GTCTCCCACTGAAAATTGCT+GGG + tig0019874:86124-86143 None:intergenic 45.0%
TGTCTCCCACTGAAAATTGC+TGG + tig0019874:86125-86144 None:intergenic 45.0%
!! CATCATGGTTTGGTGGATAC+TGG + tig0019874:86353-86372 None:intergenic 45.0%
GTATCCACCAAACCATGATG+TGG - tig0019874:86353-86372 Msa1377200:CDS 45.0%
! TCAACCACATCATGGTTTGG+TGG + tig0019874:86360-86379 None:intergenic 45.0%
TGATGTGAGAGGAATGGTTG+TGG + tig0019874:86448-86467 None:intergenic 45.0%
ACCATTCCTCTCACATCAAC+AGG - tig0019874:86450-86469 Msa1377200:CDS 45.0%
! CTTTTGCCTGTTGATGTGAG+AGG + tig0019874:86459-86478 None:intergenic 45.0%
ACAATTGGACATTGCAGCCA+AGG - tig0019874:86495-86514 Msa1377200:intron 45.0%
ACATTGCAGCCAAGGAATGA+AGG - tig0019874:86503-86522 Msa1377200:intron 45.0%
! GGAATCATTGGAGAGAGTGT+AGG + tig0019874:86708-86727 None:intergenic 45.0%
TGTTGTGCTATAGGAGAGGA+AGG + tig0019874:86750-86769 None:intergenic 45.0%
ATTTCCTTTCTCGGTCGATG+AGG + tig0019874:86790-86809 None:intergenic 45.0%
CACATTGTTGGAGGACCAAA+TGG - tig0019874:86057-86076 Msa1377200:CDS 45.0%
ATTGTTGGAGGACCAAATGG+TGG - tig0019874:86060-86079 Msa1377200:CDS 45.0%
TTGTTGGAGGACCAAATGGT+GGG - tig0019874:86061-86080 Msa1377200:CDS 45.0%
TTTGTATCCCACCCACCATT+TGG + tig0019874:86075-86094 None:intergenic 45.0%
GTCTCCCACTGAAAATTGCT+GGG + tig0019874:86124-86143 None:intergenic 45.0%
TGTCTCCCACTGAAAATTGC+TGG + tig0019874:86125-86144 None:intergenic 45.0%
!! CATCATGGTTTGGTGGATAC+TGG + tig0019874:86353-86372 None:intergenic 45.0%
GTATCCACCAAACCATGATG+TGG - tig0019874:86353-86372 Msa1377200:CDS 45.0%
! TCAACCACATCATGGTTTGG+TGG + tig0019874:86360-86379 None:intergenic 45.0%
TGATGTGAGAGGAATGGTTG+TGG + tig0019874:86448-86467 None:intergenic 45.0%
ACCATTCCTCTCACATCAAC+AGG - tig0019874:86450-86469 Msa1377200:CDS 45.0%
! CTTTTGCCTGTTGATGTGAG+AGG + tig0019874:86459-86478 None:intergenic 45.0%
ACAATTGGACATTGCAGCCA+AGG - tig0019874:86495-86514 Msa1377200:intron 45.0%
ACATTGCAGCCAAGGAATGA+AGG - tig0019874:86503-86522 Msa1377200:intron 45.0%
! GGAATCATTGGAGAGAGTGT+AGG + tig0019874:86708-86727 None:intergenic 45.0%
TGTTGTGCTATAGGAGAGGA+AGG + tig0019874:86750-86769 None:intergenic 45.0%
ATTTCCTTTCTCGGTCGATG+AGG + tig0019874:86790-86809 None:intergenic 45.0%
GGGAAGATGCCCATGATTGA+AGG + tig0019874:86104-86123 None:intergenic 50.0%
GGTTGTGGCACCATCATTGT+AGG + tig0019874:86433-86452 None:intergenic 50.0%
GCCTGTTGATGTGAGAGGAA+TGG + tig0019874:86454-86473 None:intergenic 50.0%
!! ATTGAAGGTGCTGCTGCTAC+TGG + tig0019874:86570-86589 None:intergenic 50.0%
AGGTTCTGCAGAGGGAATGA+TGG + tig0019874:86613-86632 None:intergenic 50.0%
GATTCTGCAGGTTCTGCAGA+GGG + tig0019874:86621-86640 None:intergenic 50.0%
AGATTCTGCAGGTTCTGCAG+AGG + tig0019874:86622-86641 None:intergenic 50.0%
AACAAAGGACTTGGCGCTTC+AGG + tig0019874:86666-86685 None:intergenic 50.0%
GGAGCTAGAAACTCAGTGGA+TGG + tig0019874:86729-86748 None:intergenic 50.0%
GGAAGGAGCTAGAAACTCAG+TGG + tig0019874:86733-86752 None:intergenic 50.0%
GGGAAGATGCCCATGATTGA+AGG + tig0019874:86104-86123 None:intergenic 50.0%
GGTTGTGGCACCATCATTGT+AGG + tig0019874:86433-86452 None:intergenic 50.0%
GCCTGTTGATGTGAGAGGAA+TGG + tig0019874:86454-86473 None:intergenic 50.0%
!! ATTGAAGGTGCTGCTGCTAC+TGG + tig0019874:86570-86589 None:intergenic 50.0%
AGGTTCTGCAGAGGGAATGA+TGG + tig0019874:86613-86632 None:intergenic 50.0%
GATTCTGCAGGTTCTGCAGA+GGG + tig0019874:86621-86640 None:intergenic 50.0%
AGATTCTGCAGGTTCTGCAG+AGG + tig0019874:86622-86641 None:intergenic 50.0%
AACAAAGGACTTGGCGCTTC+AGG + tig0019874:86666-86685 None:intergenic 50.0%
GGAGCTAGAAACTCAGTGGA+TGG + tig0019874:86729-86748 None:intergenic 50.0%
GGAAGGAGCTAGAAACTCAG+TGG + tig0019874:86733-86752 None:intergenic 50.0%
! TTGGAGGACCAAATGGTGGG+TGG - tig0019874:86064-86083 Msa1377200:CDS 55.0%
!! TGGAGGACCAAATGGTGGGT+GGG - tig0019874:86065-86084 Msa1377200:CDS 55.0%
! TTGGAGGACCAAATGGTGGG+TGG - tig0019874:86064-86083 Msa1377200:CDS 55.0%
!! TGGAGGACCAAATGGTGGGT+GGG - tig0019874:86065-86084 Msa1377200:CDS 55.0%
TCGGCCTCATCGACCGAGAA+AGG - tig0019874:86783-86802 Msa1377200:CDS 60.0%
TCGGCCTCATCGACCGAGAA+AGG - tig0019874:86783-86802 Msa1377200:CDS 60.0%
!! AGGTGCTGCTGCTACTGGCG+AGG + tig0019874:86565-86584 None:intergenic 65.0%
!! AGGTGCTGCTGCTACTGGCG+AGG + tig0019874:86565-86584 None:intergenic 65.0%
GCTGCTGCTACTGGCGAGGC+AGG + tig0019874:86561-86580 None:intergenic 70.0%
GCTGCTGCTACTGGCGAGGC+AGG + tig0019874:86561-86580 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0019874 gene 85985 86869 85985 ID=Msa1377200;Name=Msa1377200
tig0019874 mRNA 85985 86869 85985 ID=Msa1377200-mRNA-1;Parent=Msa1377200;Name=Msa1377200-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|229
tig0019874 exon 86704 86869 86704 ID=Msa1377200-mRNA-1:exon:5532;Parent=Msa1377200-mRNA-1
tig0019874 exon 85985 86508 85985 ID=Msa1377200-mRNA-1:exon:5531;Parent=Msa1377200-mRNA-1
tig0019874 CDS 86704 86869 86704 ID=Msa1377200-mRNA-1:cds;Parent=Msa1377200-mRNA-1
tig0019874 CDS 85985 86508 85985 ID=Msa1377200-mRNA-1:cds;Parent=Msa1377200-mRNA-1
Gene Sequence

>Msa1377200

ATGGGTGTATTTGAAATCATTCTAAGAGTATCTTTTGTGGCTATGCTAATCAAATTGGCCATGGCTACAAATCACATTGTTGGAGGACCAAATGGTGGGTGGGATACAAACTCAAACCTTCAATCATGGGCATCTTCCCAGCAATTTTCAGTGGGAGACAATCTTATTTTCCAGTATCCACCAAACCATGATGTGGTTGAAGTTACAAAAGCAGACTATGACTCCTGCCAGCAAACAAATCCAATTCAATCCTACAATGATGGTGCCACAACCATTCCTCTCACATCAACAGGCAAAAGATACTTCATTTGTGGAACAATTGGACATTGCAGCCAAGGAATGAAGGTTGAAATCGACACGCTTGCTGTTCAAGTTTCTCCTGCCTCGCCAGTAGCAGCAGCACCTTCAATAGCAGACTCTCCAATGATTTCCATCATTCCCTCTGCAGAACCTGCAGAATCTACTGTTTCATCTGCAGAATCTCCTGAAGCGCCAAGTCCTTTGTTTGAAACACAAGCGGAAAGTCCTACACTCTCTCCAATGATTCCATCCACTGAGTTTCTAGCTCCTTCCTCTCCTATAGCACAACATTCACAGGATGTATCGGCCTCATCGACCGAGAAAGGAAATCTACAAGCCTCCATTTCAGTAGTGTTGAGTTTAGTAGCGGTGTTTATGACCTTCTTCTAA
Protein sequence

>Msa1377200

MGVFEIILRVSFVAMLIKLAMATNHIVGGPNGGWDTNSNLQSWASSQQFSVGDNLIFQYPPNHDVVEVTKADYDSCQQTNPIQSYNDGATTIPLTSTGKRYFICGTIGHCSQGMKVEIDTLAVQVSPASPVAAAPSIADSPMISIIPSAEPAESTVSSAESPEAPSPLFETQAESPTLSPMIPSTEFLAPSSPIAQHSQDVSASSTEKGNLQASISVVLSLVAVFMTFF*