AlfalfaGEDB | Msa0045680

M. sativa cultivar zhongmu-4 / Msa0045680

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0045680	sp\|Q9LQQ0\|DIR25_ARATH	35.500	200	98	6	41	211	160	357	1.42e-24	103

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0045680	A0A072VS47	95.378	238	9	1	1	238	1	236	2.44e-162	457

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000370	Msa0045680	0.809573	1.769762e-50	-8.615850e-47
Msa0013270	Msa0045680	0.815063	1.119900e-51	-8.615850e-47
Msa0045680	Msa0062190	0.801858	7.382555e-49	-8.615850e-47
Msa0045680	Msa0106540	0.813425	2.576849e-51	-8.615850e-47
Msa0045680	Msa0141410	0.802844	4.625413e-49	-8.615850e-47
Msa0045680	Msa0150000	0.812096	5.035293e-51	-8.615850e-47
Msa0045680	Msa0206370	0.833390	5.481472e-56	-8.615850e-47
Msa0045680	Msa0230920	0.802419	5.661293e-49	-8.615850e-47
Msa0045680	Msa0282830	0.800032	1.743347e-48	-8.615850e-47
Msa0045680	Msa0336430	0.801549	8.542858e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0045680	MtrunA17_Chr1g0212871	95.378	238	9	1	1	238	9	244	4.77e-166	458
Msa0045680	MtrunA17_Chr1g0212861	36.441	236	104	10	43	238	123	352	1.74e-24	99.8
Msa0045680	MtrunA17_Chr4g0030681	48.571	105	47	4	110	211	178	278	1.47e-22	93.6
Msa0045680	MtrunA17_Chr4g0030731	48.571	105	47	4	110	211	177	277	2.07e-22	93.2
Msa0045680	MtrunA17_Chr7g0221601	43.443	122	63	2	116	233	267	386	5.73e-22	93.2
Msa0045680	MtrunA17_Chr1g0169511	32.057	209	110	5	43	226	39	240	2.27e-21	89.4
Msa0045680	MtrunA17_Chr1g0169291	32.536	209	109	6	43	226	39	240	2.39e-20	86.7
Msa0045680	MtrunA17_Chr4g0030671	32.000	200	106	6	43	214	61	258	5.88e-18	80.9
Msa0045680	MtrunA17_Chr1g0169281	27.490	251	147	5	4	226	3	246	6.36e-17	77.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0045680	AT1G07730.2	35.500	200	98	6	41	211	160	357	1.44e-25	103
Msa0045680	AT2G28670.2	51.887	106	49	2	106	211	91	194	7.72e-25	98.6
Msa0045680	AT2G28670.1	35.644	202	97	5	41	211	205	404	9.94e-25	101
Msa0045680	AT4G13580.1	33.871	186	98	4	62	224	49	232	8.07e-23	93.6
Msa0045680	AT3G24020.1	32.864	213	113	6	47	236	38	243	1.92e-22	92.4
Msa0045680	AT2G39430.1	31.933	238	114	9	43	236	87	320	9.68e-22	92.0
Msa0045680	AT3G55230.1	30.252	238	120	8	43	237	70	304	2.43e-20	88.2

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Find 51 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAACTTGGAAAAGCACA+AGG	0.629760	1_1:-77629467	Msa0045680:CDS
ACACTTGATCTTTCCACCAT+TGG	0.449523	1_1:-77629593	Msa0045680:CDS
AGAAACTTGGAAAAGCACAA+GGG	0.691717	1_1:-77629466	Msa0045680:CDS
AGAGAAACCTTCCACTACTA+AGG	0.495734	1_1:-77629702	Msa0045680:CDS
AGAGGATGGAAGTAGCCATA+TGG	0.562281	1_1:-77629426	Msa0045680:CDS
AGCTGAGGAAAATTTGTTTA+TGG	0.235026	1_1:+77629832	None:intergenic
AGGAAAAGAGAAACCAATGG+TGG	0.644162	1_1:+77629580	None:intergenic
AGTAGGAAAAGAGAAACCAA+TGG	0.647659	1_1:+77629577	None:intergenic
ATCAGTAACATCGATCGACG+AGG	0.719628	1_1:-77629525	Msa0045680:CDS
ATGGCCATGGTAGTGATTAG+GGG	0.679512	1_1:+77629790	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGATGAACTAAAGAAACT+TGG	-	chr1_1:77629601-77629620	Msa0045680:CDS	30.0%
TTAAGGTTGTGGATAAAGTA+GGG	-	chr1_1:77629821-77629840	Msa0045680:CDS	30.0%
TAAAATACACGTCATACAGA+AGG	+	chr1_1:77629885-77629904	None:intergenic	30.0%
TGACAATTGCTAGAAGAGAA+GGG	+	chr1_1:77629207-77629226	None:intergenic	35.0%
ATGTTATTCTATGATGGCCA+TGG	+	chr1_1:77629306-77629325	None:intergenic	35.0%
TTTGGAGGAAAAAATCCTAG+AGG	+	chr1_1:77629430-77629449	None:intergenic	35.0%
AGTAGGAAAAGAGAAACCAA+TGG	+	chr1_1:77629506-77629525	None:intergenic	35.0%
TAATGGCTATGCTGTTGTTA+AGG	-	chr1_1:77629804-77629823	Msa0045680:CDS	35.0%
GTTAAGGTTGTGGATAAAGT+AGG	-	chr1_1:77629820-77629839	Msa0045680:CDS	35.0%
CTGACAATTGCTAGAAGAGA+AGG	+	chr1_1:77629208-77629227	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	77629193	77629909	77629193	ID=Msa0045680;Name=Msa0045680
chr1_1	mRNA	77629193	77629909	77629193	ID=Msa0045680-mRNA-1;Parent=Msa0045680;Name=Msa0045680-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|238
chr1_1	exon	77629193	77629909	77629193	ID=Msa0045680-mRNA-1:exon:26596;Parent=Msa0045680-mRNA-1
chr1_1	CDS	77629193	77629909	77629193	ID=Msa0045680-mRNA-1:cds;Parent=Msa0045680-mRNA-1

Gene Sequence

>Msa0045680

ATGGCCAATCTCCCTTCTCTTCTAGCAATTGTCAGGTTACTTTTGCTAATGACTACCATAAACAAATTTTCCTCAGCTAGAACTCTTGGTAATCCAACCCCTAATCACTACCATGGCCATCATAGAATAACATTTTTAATGAAGCATATGCTCAATGATACACATCCTAGTCCTAATATTAATACAGAGAAACCTTCCACTACTAAGGTCACAAATCAATTACCTTTTCCAAAACCTCTAGGATTTTTTCCTCCAAATGGAGGGATTCCAATTCCACAATCAACTCAAACACAAACACTTGATCTTTCCACCATTGGTTTCTCTTTTCCTACTAGAGCAGCATTTCAAGAGCTGGAATTTGGATCAGTAACATCGATCGACGAGGAACTACTAGTTGAAGGTGATCATGAAGATGAACTAAAGAAACTTGGAAAAGCACAAGGGGTGTATGTAGCTAGTGAAGAGGATGGAAGTAGCCATATGGTTGCTATGACAGTGAGTATTTTGAAGGGTGGGTATGAAGACGGGTTGAGACTTTTTGGAGTTCATAGGAGTGATGTTTTTGAGTCACATGTTGCAGTTATTGGTGGTACTGGAAAGTACCATGGTGCTAATGGCTATGCTGTTGTTAAGGTTGTGGATAAAGTAGGGTCTAGTACAGTAGAAGGAAAAGTTACAAGTTCTAAGTTCCTTCTGTATGACGTGTATTTTAGTTAG

Protein sequence

>Msa0045680

MANLPSLLAIVRLLLLMTTINKFSSARTLGNPTPNHYHGHHRITFLMKHMLNDTHPSPNINTEKPSTTKVTNQLPFPKPLGFFPPNGGIPIPQSTQTQTLDLSTIGFSFPTRAAFQELEFGSVTSIDEELLVEGDHEDELKKLGKAQGVYVASEEDGSSHMVAMTVSILKGGYEDGLRLFGVHRSDVFESHVAVIGGTGKYHGANGYAVVKVVDKVGSSTVEGKVTSSKFLLYDVYFS*