AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0045680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045680 MtrunA17_Chr1g0212871 95.378 238 9 1 1 238 9 244 4.77e-166 458
Msa0045680 MtrunA17_Chr1g0212861 36.441 236 104 10 43 238 123 352 1.74e-24 99.8
Msa0045680 MtrunA17_Chr4g0030681 48.571 105 47 4 110 211 178 278 1.47e-22 93.6
Msa0045680 MtrunA17_Chr4g0030731 48.571 105 47 4 110 211 177 277 2.07e-22 93.2
Msa0045680 MtrunA17_Chr7g0221601 43.443 122 63 2 116 233 267 386 5.73e-22 93.2
Msa0045680 MtrunA17_Chr1g0169511 32.057 209 110 5 43 226 39 240 2.27e-21 89.4
Msa0045680 MtrunA17_Chr1g0169291 32.536 209 109 6 43 226 39 240 2.39e-20 86.7
Msa0045680 MtrunA17_Chr4g0030671 32.000 200 106 6 43 214 61 258 5.88e-18 80.9
Msa0045680 MtrunA17_Chr1g0169281 27.490 251 147 5 4 226 3 246 6.36e-17 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045680 AT1G07730.2 35.500 200 98 6 41 211 160 357 1.44e-25 103
Msa0045680 AT2G28670.2 51.887 106 49 2 106 211 91 194 7.72e-25 98.6
Msa0045680 AT2G28670.1 35.644 202 97 5 41 211 205 404 9.94e-25 101
Msa0045680 AT4G13580.1 33.871 186 98 4 62 224 49 232 8.07e-23 93.6
Msa0045680 AT3G24020.1 32.864 213 113 6 47 236 38 243 1.92e-22 92.4
Msa0045680 AT2G39430.1 31.933 238 114 9 43 236 87 320 9.68e-22 92.0
Msa0045680 AT3G55230.1 30.252 238 120 8 43 237 70 304 2.43e-20 88.2

Find 51 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTTCAAGAGCTGGAATT+TGG 0.209367 1_1:-77629548 Msa0045680:CDS
GGTTTCTCTGTATTAATATT+AGG 0.221299 1_1:+77629716 None:intergenic
AGCTGAGGAAAATTTGTTTA+TGG 0.235026 1_1:+77629832 None:intergenic
GATGGCCATGGTAGTGATTA+GGG 0.312185 1_1:+77629789 None:intergenic
GTTTGAGTTGATTGTGGAAT+TGG 0.329326 1_1:+77629620 None:intergenic
TGATGGCCATGGTAGTGATT+AGG 0.343261 1_1:+77629788 None:intergenic
GAGTCACATGTTGCAGTTAT+TGG 0.351309 1_1:-77629323 Msa0045680:CDS
CTTCTCTTCTAGCAATTGTC+AGG 0.383847 1_1:-77629874 Msa0045680:CDS
GGAATTGGAATCCCTCCATT+TGG 0.399195 1_1:+77629635 None:intergenic
GTTAAGGTTGTGGATAAAGT+AGG 0.408640 1_1:-77629260 Msa0045680:CDS
GTAGGGTCTAGTACAGTAGA+AGG 0.416844 1_1:-77629242 Msa0045680:CDS
GGAAAGTACCATGGTGCTAA+TGG 0.420735 1_1:-77629293 Msa0045680:CDS
TCTTGAAATGCTGCTCTAGT+AGG 0.424130 1_1:+77629560 None:intergenic
TGAAGGGTGGGTATGAAGAC+GGG 0.437856 1_1:-77629382 Msa0045680:CDS
TAATGGCTATGCTGTTGTTA+AGG 0.440993 1_1:-77629276 Msa0045680:CDS
ACACTTGATCTTTCCACCAT+TGG 0.449523 1_1:-77629593 Msa0045680:CDS
GTTGCAGTTATTGGTGGTAC+TGG 0.455685 1_1:-77629314 Msa0045680:CDS
TTGAAGGGTGGGTATGAAGA+CGG 0.463306 1_1:-77629383 Msa0045680:CDS
TGCTAGAAGAGAAGGGAGAT+TGG 0.464219 1_1:+77629883 None:intergenic
CTATGCTGTTGTTAAGGTTG+TGG 0.492544 1_1:-77629270 Msa0045680:CDS
AGAGAAACCTTCCACTACTA+AGG 0.495734 1_1:-77629702 Msa0045680:CDS
CTGACAATTGCTAGAAGAGA+AGG 0.504357 1_1:+77629875 None:intergenic
TAGAGCAGCATTTCAAGAGC+TGG 0.504564 1_1:-77629555 Msa0045680:CDS
GACGAGGAACTACTAGTTGA+AGG 0.506266 1_1:-77629509 Msa0045680:CDS
TATGTAGCTAGTGAAGAGGA+TGG 0.509172 1_1:-77629440 Msa0045680:CDS
GTTTGTGTTTGAGTTGATTG+TGG 0.515184 1_1:+77629614 None:intergenic
TTAAGGTTGTGGATAAAGTA+GGG 0.516469 1_1:-77629259 Msa0045680:CDS
TCACTGTCATAGCAACCATA+TGG 0.517871 1_1:+77629411 None:intergenic
GAAGATGAACTAAAGAAACT+TGG 0.528902 1_1:-77629479 Msa0045680:CDS
TCTGTATTAATATTAGGACT+AGG 0.530173 1_1:+77629722 None:intergenic
ATTGGAATCCCTCCATTTGG+AGG 0.538267 1_1:+77629638 None:intergenic
CCATGGTAGTGATTAGGGGT+TGG 0.550854 1_1:+77629794 None:intergenic
AGAGGATGGAAGTAGCCATA+TGG 0.562281 1_1:-77629426 Msa0045680:CDS
TTTGTGACCTTAGTAGTGGA+AGG 0.562764 1_1:+77629695 None:intergenic
TTGATTTGTGACCTTAGTAG+TGG 0.562901 1_1:+77629691 None:intergenic
ATGTTATTCTATGATGGCCA+TGG 0.591384 1_1:+77629777 None:intergenic
GGTGTATGTAGCTAGTGAAG+AGG 0.591556 1_1:-77629444 Msa0045680:CDS
TAAAATACACGTCATACAGA+AGG 0.595028 1_1:+77629198 None:intergenic
TCACATGTTGCAGTTATTGG+TGG 0.626055 1_1:-77629320 Msa0045680:CDS
AAGAAACTTGGAAAAGCACA+AGG 0.629760 1_1:-77629467 Msa0045680:CDS
TGACAATTGCTAGAAGAGAA+GGG 0.634492 1_1:+77629876 None:intergenic
AGGAAAAGAGAAACCAATGG+TGG 0.644162 1_1:+77629580 None:intergenic
AGTAGGAAAAGAGAAACCAA+TGG 0.647659 1_1:+77629577 None:intergenic
CCAACCCCTAATCACTACCA+TGG 0.665385 1_1:-77629794 Msa0045680:CDS
GAAACTTGGAAAAGCACAAG+GGG 0.665476 1_1:-77629465 Msa0045680:CDS
CAGCATAGCCATTAGCACCA+TGG 0.679074 1_1:+77629285 None:intergenic
ATGGCCATGGTAGTGATTAG+GGG 0.679512 1_1:+77629790 None:intergenic
AGAAACTTGGAAAAGCACAA+GGG 0.691717 1_1:-77629466 Msa0045680:CDS
ATTACCAAGAGTTCTAGCTG+AGG 0.711799 1_1:+77629817 None:intergenic
ATCAGTAACATCGATCGACG+AGG 0.719628 1_1:-77629525 Msa0045680:CDS
GGTGGTACTGGAAAGTACCA+TGG 0.742516 1_1:-77629302 Msa0045680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAATGTTATTCTATGA+TGG + chr1_1:77629312-77629331 None:intergenic 15.0%
! TCTGTATTAATATTAGGACT+AGG + chr1_1:77629361-77629380 None:intergenic 25.0%
! GGTTTCTCTGTATTAATATT+AGG + chr1_1:77629367-77629386 None:intergenic 25.0%
! AGCTGAGGAAAATTTGTTTA+TGG + chr1_1:77629251-77629270 None:intergenic 30.0%
GAAGATGAACTAAAGAAACT+TGG - chr1_1:77629601-77629620 Msa0045680:CDS 30.0%
!! TATGACAGTGAGTATTTTGA+AGG - chr1_1:77629681-77629700 Msa0045680:CDS 30.0%
!! ATGACAGTGAGTATTTTGAA+GGG - chr1_1:77629682-77629701 Msa0045680:CDS 30.0%
TTAAGGTTGTGGATAAAGTA+GGG - chr1_1:77629821-77629840 Msa0045680:CDS 30.0%
TAAAATACACGTCATACAGA+AGG + chr1_1:77629885-77629904 None:intergenic 30.0%
TGACAATTGCTAGAAGAGAA+GGG + chr1_1:77629207-77629226 None:intergenic 35.0%
ATGTTATTCTATGATGGCCA+TGG + chr1_1:77629306-77629325 None:intergenic 35.0%
!! TTGATTTGTGACCTTAGTAG+TGG + chr1_1:77629392-77629411 None:intergenic 35.0%
! TTACCTTTTCCAAAACCTCT+AGG - chr1_1:77629412-77629431 Msa0045680:CDS 35.0%
!!! AATCCTAGAGGTTTTGGAAA+AGG + chr1_1:77629418-77629437 None:intergenic 35.0%
! GGAAAAAATCCTAGAGGTTT+TGG + chr1_1:77629424-77629443 None:intergenic 35.0%
TTTGGAGGAAAAAATCCTAG+AGG + chr1_1:77629430-77629449 None:intergenic 35.0%
!!! CTAGGATTTTTTCCTCCAAA+TGG - chr1_1:77629430-77629449 Msa0045680:CDS 35.0%
!!! GATTTTTTCCTCCAAATGGA+GGG - chr1_1:77629434-77629453 Msa0045680:CDS 35.0%
!! GTTTGAGTTGATTGTGGAAT+TGG + chr1_1:77629463-77629482 None:intergenic 35.0%
!! GTTTGTGTTTGAGTTGATTG+TGG + chr1_1:77629469-77629488 None:intergenic 35.0%
AGTAGGAAAAGAGAAACCAA+TGG + chr1_1:77629506-77629525 None:intergenic 35.0%
! AAGAAACTTGGAAAAGCACA+AGG - chr1_1:77629613-77629632 Msa0045680:CDS 35.0%
! AGAAACTTGGAAAAGCACAA+GGG - chr1_1:77629614-77629633 Msa0045680:CDS 35.0%
!!! TGAGACTTTTTGGAGTTCAT+AGG - chr1_1:77629722-77629741 Msa0045680:CDS 35.0%
TAATGGCTATGCTGTTGTTA+AGG - chr1_1:77629804-77629823 Msa0045680:CDS 35.0%
GTTAAGGTTGTGGATAAAGT+AGG - chr1_1:77629820-77629839 Msa0045680:CDS 35.0%
CTGACAATTGCTAGAAGAGA+AGG + chr1_1:77629208-77629227 None:intergenic 40.0%
CTTCTCTTCTAGCAATTGTC+AGG - chr1_1:77629206-77629225 Msa0045680:CDS 40.0%
! TTTTCCTCAGCTAGAACTCT+TGG - chr1_1:77629259-77629278 Msa0045680:CDS 40.0%
! ATTACCAAGAGTTCTAGCTG+AGG + chr1_1:77629266-77629285 None:intergenic 40.0%
AGAGAAACCTTCCACTACTA+AGG - chr1_1:77629378-77629397 Msa0045680:CDS 40.0%
! TTTGTGACCTTAGTAGTGGA+AGG + chr1_1:77629388-77629407 None:intergenic 40.0%
!!! GGATTTTTTCCTCCAAATGG+AGG - chr1_1:77629433-77629452 Msa0045680:CDS 40.0%
ACACTTGATCTTTCCACCAT+TGG - chr1_1:77629487-77629506 Msa0045680:CDS 40.0%
AGGAAAAGAGAAACCAATGG+TGG + chr1_1:77629503-77629522 None:intergenic 40.0%
TCTTGAAATGCTGCTCTAGT+AGG + chr1_1:77629523-77629542 None:intergenic 40.0%
GCATTTCAAGAGCTGGAATT+TGG - chr1_1:77629532-77629551 Msa0045680:CDS 40.0%
! GAAACTTGGAAAAGCACAAG+GGG - chr1_1:77629615-77629634 Msa0045680:CDS 40.0%
TATGTAGCTAGTGAAGAGGA+TGG - chr1_1:77629640-77629659 Msa0045680:CDS 40.0%
TCACTGTCATAGCAACCATA+TGG + chr1_1:77629672-77629691 None:intergenic 40.0%
!! ACAGTGAGTATTTTGAAGGG+TGG - chr1_1:77629685-77629704 Msa0045680:CDS 40.0%
!! CAGTGAGTATTTTGAAGGGT+GGG - chr1_1:77629686-77629705 Msa0045680:CDS 40.0%
GAGTCACATGTTGCAGTTAT+TGG - chr1_1:77629757-77629776 Msa0045680:CDS 40.0%
TCACATGTTGCAGTTATTGG+TGG - chr1_1:77629760-77629779 Msa0045680:CDS 40.0%
CTATGCTGTTGTTAAGGTTG+TGG - chr1_1:77629810-77629829 Msa0045680:CDS 40.0%
TGCTAGAAGAGAAGGGAGAT+TGG + chr1_1:77629200-77629219 None:intergenic 45.0%
! ATGGCCATGGTAGTGATTAG+GGG + chr1_1:77629293-77629312 None:intergenic 45.0%
! GATGGCCATGGTAGTGATTA+GGG + chr1_1:77629294-77629313 None:intergenic 45.0%
! TGATGGCCATGGTAGTGATT+AGG + chr1_1:77629295-77629314 None:intergenic 45.0%
ATTGGAATCCCTCCATTTGG+AGG + chr1_1:77629445-77629464 None:intergenic 45.0%
GGAATTGGAATCCCTCCATT+TGG + chr1_1:77629448-77629467 None:intergenic 45.0%
TAGAGCAGCATTTCAAGAGC+TGG - chr1_1:77629525-77629544 Msa0045680:CDS 45.0%
ATCAGTAACATCGATCGACG+AGG - chr1_1:77629555-77629574 Msa0045680:CDS 45.0%
GACGAGGAACTACTAGTTGA+AGG - chr1_1:77629571-77629590 Msa0045680:CDS 45.0%
GGTGTATGTAGCTAGTGAAG+AGG - chr1_1:77629636-77629655 Msa0045680:CDS 45.0%
AGAGGATGGAAGTAGCCATA+TGG - chr1_1:77629654-77629673 Msa0045680:CDS 45.0%
TTGAAGGGTGGGTATGAAGA+CGG - chr1_1:77629697-77629716 Msa0045680:CDS 45.0%
!!! GAAGACGGGTTGAGACTTTT+TGG - chr1_1:77629712-77629731 Msa0045680:CDS 45.0%
! GTTGCAGTTATTGGTGGTAC+TGG - chr1_1:77629766-77629785 Msa0045680:CDS 45.0%
!! GGAAAGTACCATGGTGCTAA+TGG - chr1_1:77629787-77629806 Msa0045680:CDS 45.0%
GTAGGGTCTAGTACAGTAGA+AGG - chr1_1:77629838-77629857 Msa0045680:CDS 45.0%
! CCATGGTAGTGATTAGGGGT+TGG + chr1_1:77629289-77629308 None:intergenic 50.0%
CCAACCCCTAATCACTACCA+TGG - chr1_1:77629286-77629305 Msa0045680:CDS 50.0%
TGAAGGGTGGGTATGAAGAC+GGG - chr1_1:77629698-77629717 Msa0045680:CDS 50.0%
!! GGTGGTACTGGAAAGTACCA+TGG - chr1_1:77629778-77629797 Msa0045680:CDS 50.0%
CAGCATAGCCATTAGCACCA+TGG + chr1_1:77629798-77629817 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 77629193 77629909 77629193 ID=Msa0045680;Name=Msa0045680
chr1_1 mRNA 77629193 77629909 77629193 ID=Msa0045680-mRNA-1;Parent=Msa0045680;Name=Msa0045680-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|238
chr1_1 exon 77629193 77629909 77629193 ID=Msa0045680-mRNA-1:exon:26596;Parent=Msa0045680-mRNA-1
chr1_1 CDS 77629193 77629909 77629193 ID=Msa0045680-mRNA-1:cds;Parent=Msa0045680-mRNA-1
Gene Sequence

>Msa0045680

ATGGCCAATCTCCCTTCTCTTCTAGCAATTGTCAGGTTACTTTTGCTAATGACTACCATAAACAAATTTTCCTCAGCTAGAACTCTTGGTAATCCAACCCCTAATCACTACCATGGCCATCATAGAATAACATTTTTAATGAAGCATATGCTCAATGATACACATCCTAGTCCTAATATTAATACAGAGAAACCTTCCACTACTAAGGTCACAAATCAATTACCTTTTCCAAAACCTCTAGGATTTTTTCCTCCAAATGGAGGGATTCCAATTCCACAATCAACTCAAACACAAACACTTGATCTTTCCACCATTGGTTTCTCTTTTCCTACTAGAGCAGCATTTCAAGAGCTGGAATTTGGATCAGTAACATCGATCGACGAGGAACTACTAGTTGAAGGTGATCATGAAGATGAACTAAAGAAACTTGGAAAAGCACAAGGGGTGTATGTAGCTAGTGAAGAGGATGGAAGTAGCCATATGGTTGCTATGACAGTGAGTATTTTGAAGGGTGGGTATGAAGACGGGTTGAGACTTTTTGGAGTTCATAGGAGTGATGTTTTTGAGTCACATGTTGCAGTTATTGGTGGTACTGGAAAGTACCATGGTGCTAATGGCTATGCTGTTGTTAAGGTTGTGGATAAAGTAGGGTCTAGTACAGTAGAAGGAAAAGTTACAAGTTCTAAGTTCCTTCTGTATGACGTGTATTTTAGTTAG

Protein sequence

>Msa0045680

MANLPSLLAIVRLLLLMTTINKFSSARTLGNPTPNHYHGHHRITFLMKHMLNDTHPSPNINTEKPSTTKVTNQLPFPKPLGFFPPNGGIPIPQSTQTQTLDLSTIGFSFPTRAAFQELEFGSVTSIDEELLVEGDHEDELKKLGKAQGVYVASEEDGSSHMVAMTVSILKGGYEDGLRLFGVHRSDVFESHVAVIGGTGKYHGANGYAVVKVVDKVGSSTVEGKVTSSKFLLYDVYFS*