AlfalfaGEDB | Msa1389140

M. sativa cultivar zhongmu-4 / Msa1389140

KEGG: K08065
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0010228 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048571 GO:0048573 GO:0048574 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389140	XP_013458655.1	95.588	136	6	0	1	136	1	136	6.94e-90	267

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389140	sp\|O23310\|NFYB3_ARATH	75.424	118	23	2	1	114	1	116	4.73e-59	182

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389140	I3TAW6	95.588	136	6	0	1	136	1	136	3.31e-90	267

TFs/TRs:

Gene ID	Type	Classification
Msa1389140	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0034970	Msa1389140	0.809021	2.324713e-50	-8.615850e-47
Msa0104800	Msa1389140	0.805381	1.372027e-49	-8.615850e-47
Msa1370590	Msa1389140	0.806586	7.656681e-50	-8.615850e-47
Msa1384100	Msa1389140	0.810126	1.345859e-50	-8.615850e-47
Msa1389140	Msa1397120	0.801674	8.053823e-49	-8.615850e-47
Msa1389140	Msa1403890	0.802399	5.713820e-49	-8.615850e-47
Msa1389140	Msa1444120	0.803029	4.236868e-49	-8.615850e-47
Msa0709160	Msa1389140	0.800934	1.141563e-48	-8.615850e-47
Msa0732880	Msa1389140	0.804287	2.322101e-49	-8.615850e-47
Msa0175840	Msa1389140	0.828411	9.126349e-55	-8.615850e-47
Msa0206010	Msa1389140	0.807026	6.179499e-50	-8.615850e-47
Msa0207030	Msa1389140	0.800117	1.675411e-48	-8.615850e-47
Msa1250530	Msa1389140	0.802093	6.606242e-49	-8.615850e-47
Msa1337170	Msa1389140	0.868010	8.968063e-66	-8.615850e-47
Msa0401560	Msa1389140	0.800076	1.707835e-48	-8.615850e-47
Msa0482040	Msa1389140	0.800199	1.611905e-48	-8.615850e-47
Msa0980880	Msa1389140	0.812324	4.489064e-51	-8.615850e-47
Msa0326110	Msa1389140	0.805712	1.169229e-49	-8.615850e-47
Msa0482940	Msa1389140	0.809352	1.974640e-50	-8.615850e-47
Msa0566350	Msa1389140	0.806857	6.709043e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389140	MtrunA17_Chr4g0076321	95.588	136	6	0	1	136	1	136	6.38e-94	267
Msa1389140	MtrunA17_Chr2g0290491	75.385	130	27	2	1	130	1	125	9.07e-65	194
Msa1389140	MtrunA17_Chr5g0446491	75.000	120	26	2	1	116	1	120	1.26e-59	182
Msa1389140	MtrunA17_Chr3g0102351	71.901	121	26	1	1	113	1	121	3.35e-59	181
Msa1389140	MtrunA17_Chr8g0382931	81.443	97	18	0	20	116	29	125	1.96e-54	169
Msa1389140	MtrunA17_Chr8g0384451	79.787	94	19	0	20	113	41	134	2.76e-54	169
Msa1389140	MtrunA17_Chr1g0185911	69.748	119	31	3	10	127	22	136	3.77e-52	162
Msa1389140	MtrunA17_Chr7g0263601	65.079	126	41	1	7	129	14	139	3.96e-52	162
Msa1389140	MtrunA17_Chr2g0296321	68.750	112	29	2	6	113	51	160	6.54e-52	164
Msa1389140	MtrunA17_Chr1g0195851	74.468	94	24	0	20	113	37	130	1.22e-51	162
Msa1389140	MtrunA17_Chr4g0067091	69.159	107	28	1	4	110	12	113	4.63e-51	161
Msa1389140	MtrunA17_Chr4g0076381	67.021	94	31	0	20	113	59	152	1.25e-45	148
Msa1389140	MtrunA17_Chr1g0191981	54.867	113	45	1	21	133	6	112	3.28e-42	136
Msa1389140	MtrunA17_Chr1g0165041	57.426	101	41	1	13	113	2	100	5.02e-40	132
Msa1389140	MtrunA17_Chr5g0446601	44.144	111	57	2	1	108	5	113	1.41e-24	93.2
Msa1389140	MtrunA17_Chr1g0159271	53.165	79	37	0	33	111	1	79	7.14e-24	89.0
Msa1389140	MtrunA17_Chr1g0159291	40.789	76	45	0	24	99	7	82	4.63e-19	76.3
Msa1389140	MtrunA17_Chr1g0158951	51.515	66	32	0	49	114	19	84	4.88e-19	76.6
Msa1389140	MtrunA17_Chr2g0307551	30.952	84	58	0	24	107	16	99	8.66e-14	64.3
Msa1389140	MtrunA17_Chr4g0062271	30.952	84	58	0	24	107	16	99	3.02e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389140	AT4G14540.1	75.424	118	23	2	1	114	1	116	4.81e-60	182
Msa1389140	AT5G47640.1	82.105	95	17	0	20	114	28	122	4.74e-55	171
Msa1389140	AT2G47810.1	76.596	94	22	0	20	113	52	145	5.27e-52	162
Msa1389140	AT2G38880.3	65.323	124	39	3	9	130	12	133	7.55e-52	161
Msa1389140	AT2G38880.2	65.323	124	39	3	9	130	12	133	1.16e-51	160
Msa1389140	AT2G38880.1	65.323	124	39	3	9	130	12	133	1.16e-51	160
Msa1389140	AT2G38880.5	65.323	124	39	3	9	130	12	133	1.16e-51	160
Msa1389140	AT2G13570.1	76.596	94	22	0	20	113	37	130	2.05e-51	162
Msa1389140	AT3G53340.5	74.194	93	24	0	20	112	30	122	3.47e-50	158
Msa1389140	AT3G53340.1	74.194	93	24	0	20	112	30	122	3.47e-50	158
Msa1389140	AT2G37060.2	69.725	109	29	2	7	112	16	123	4.07e-50	158
Msa1389140	AT2G37060.3	69.725	109	29	2	7	112	16	123	4.07e-50	158
Msa1389140	AT2G37060.1	69.725	109	29	2	7	112	16	123	4.07e-50	158
Msa1389140	AT2G38880.4	72.549	102	25	2	9	109	12	111	2.22e-49	154
Msa1389140	AT2G38880.9	72.549	102	25	2	9	109	12	111	3.65e-49	154
Msa1389140	AT2G38880.10	72.549	102	25	2	9	109	12	111	3.65e-49	154
Msa1389140	AT2G38880.6	72.549	102	25	2	9	109	12	111	3.65e-49	154
Msa1389140	AT2G38880.7	63.415	123	41	3	9	130	12	131	6.26e-49	154
Msa1389140	AT2G38880.11	72.549	102	25	2	9	109	12	111	7.58e-49	154
Msa1389140	AT2G38880.8	72.549	102	25	2	9	109	12	111	7.58e-49	154
Msa1389140	AT3G53340.4	73.333	90	24	0	20	109	30	119	1.02e-48	152
Msa1389140	AT3G53340.3	73.626	91	24	0	20	110	30	120	3.19e-48	152
Msa1389140	AT3G53340.2	73.626	91	24	0	20	110	30	120	3.19e-48	152
Msa1389140	AT5G47670.2	63.918	97	35	0	20	116	30	126	6.32e-45	145
Msa1389140	AT5G47670.1	63.918	97	35	0	20	116	59	155	1.43e-44	145
Msa1389140	AT5G47670.3	63.918	97	35	0	20	116	59	155	1.43e-44	145
Msa1389140	AT1G09030.1	57.391	115	46	1	20	131	4	118	2.39e-44	142
Msa1389140	AT1G21970.1	58.163	98	41	0	20	117	60	157	2.82e-41	137
Msa1389140	AT5G08190.2	35.052	97	61	1	24	118	16	112	7.86e-18	75.5
Msa1389140	AT5G08190.1	34.694	98	61	2	24	118	16	113	4.53e-16	70.9
Msa1389140	AT5G23090.4	30.928	97	65	1	24	118	16	112	1.06e-14	67.0
Msa1389140	AT5G23090.5	34.118	85	54	1	24	106	16	100	5.97e-14	65.1
Msa1389140	AT5G23090.2	34.118	85	54	1	24	106	16	100	5.97e-14	65.1
Msa1389140	AT5G23090.1	34.118	85	54	1	24	106	16	100	5.97e-14	65.1
Msa1389140	AT5G23090.3	32.941	85	54	2	25	106	3	87	8.89e-11	56.6

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATCAGGTGGTAAAGCTTC+AGG	0.307857	tig0021212:+96145	Msa1389140:CDS
GGGTTACCTTCACAAGTATA+GGG	0.382046	tig0021212:+96426	Msa1389140:CDS
GATCATGAAGAAAGCTTTAC+CGG	0.392387	tig0021212:+96219	Msa1389140:CDS
AGAATATGCAGAGCCTCTTA+AGG	0.393028	tig0021212:+96405	Msa1389140:CDS
AGGGTTACCTTCACAAGTAT+AGG	0.429699	tig0021212:+96425	Msa1389140:CDS
TTCGATATCTTTGCTCTCGC+CGG	0.458504	tig0021212:-96238	None:intergenic
GAGTTCATAAGCTTCATAAC+AGG	0.460674	tig0021212:+96295	Msa1389140:CDS
GATCCTACCAACGTTTGCTA+TGG	0.475579	tig0021212:-96200	None:intergenic
GCAGAATCAGATGATGAATC+AGG	0.475967	tig0021212:+96130	Msa1389140:CDS
GAGAAGAGGAAGACAATAAA+CGG	0.477283	tig0021212:+96343	Msa1389140:CDS
TTGTGAAGGTAACCCTTAAG+AGG	0.483388	tig0021212:-96418	None:intergenic
CAATTTCCCTATACTTGTGA+AGG	0.485169	tig0021212:-96432	None:intergenic
GAATATGCAGAGCCTCTTAA+GGG	0.488554	tig0021212:+96406	Msa1389140:CDS
GAATATGATTGGTAGTAGTA+AGG	0.514281	tig0021212:+96477	Msa1389140:CDS
GCAGAGAATTACTGCAAGAA+AGG	0.517934	tig0021212:+96170	Msa1389140:CDS
AATCAGGTGGTAAAGCTTCA+GGG	0.531597	tig0021212:+96146	Msa1389140:CDS
CACAAGTATAGGGAAATTGA+AGG	0.531636	tig0021212:+96436	Msa1389140:CDS
GTTGCAAATATAATCAACCT+TGG	0.534009	tig0021212:-96528	None:intergenic
ATCCTACCAACGTTTGCTAT+GGG	0.537868	tig0021212:-96199	None:intergenic
AAAAGTGTCAGAAAGAGAAG+AGG	0.551950	tig0021212:+96329	Msa1389140:CDS
AGTATTGATCGATTCTTCCA+AGG	0.558864	tig0021212:+96511	Msa1389140:CDS
TTCAAAGCCAAGTGTAGTCA+TGG	0.563942	tig0021212:-96383	None:intergenic
GTGTGGGCCATGACTACACT+TGG	0.565546	tig0021212:+96376	Msa1389140:CDS
TACCAACGTTTGCTATGGGA+AGG	0.599059	tig0021212:-96195	None:intergenic
TTCCCATAGCAAACGTTGGT+AGG	0.621000	tig0021212:+96197	Msa1389140:CDS
CTCCTTCCCATAGCAAACGT+TGG	0.626066	tig0021212:+96193	Msa1389140:CDS
GGTAGTAGTAAGGAGCAGCA+AGG	0.645799	tig0021212:+96487	Msa1389140:CDS
TAAACGGTGATGATCTTGTG+TGG	0.661494	tig0021212:+96359	Msa1389140:CDS
AAACGGTGATGATCTTGTGT+GGG	0.691215	tig0021212:+96360	Msa1389140:CDS
GAATCAGATGATGAATCAGG+TGG	0.708914	tig0021212:+96133	Msa1389140:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTTCTATGAATATGAT+TGG	+	tig0021212:96466-96485	Msa1389140:CDS	15.0%
!	GAATATGATTGGTAGTAGTA+AGG	+	tig0021212:96477-96496	Msa1389140:CDS	30.0%
	GATCATGAAGAAAGCTTTAC+CGG	+	tig0021212:96219-96238	Msa1389140:CDS	35.0%
	GAGTTCATAAGCTTCATAAC+AGG	+	tig0021212:96295-96314	Msa1389140:CDS	35.0%
!!	AAAAGTGTCAGAAAGAGAAG+AGG	+	tig0021212:96329-96348	Msa1389140:CDS	35.0%
	GAGAAGAGGAAGACAATAAA+CGG	+	tig0021212:96343-96362	Msa1389140:CDS	35.0%
	CAATTTCCCTATACTTGTGA+AGG	-	tig0021212:96435-96454	None:intergenic	35.0%
	CACAAGTATAGGGAAATTGA+AGG	+	tig0021212:96436-96455	Msa1389140:CDS	35.0%
!!	AGTATTGATCGATTCTTCCA+AGG	+	tig0021212:96511-96530	Msa1389140:CDS	35.0%
	GCAGAATCAGATGATGAATC+AGG	+	tig0021212:96130-96149	Msa1389140:CDS	40.0%
	GAATCAGATGATGAATCAGG+TGG	+	tig0021212:96133-96152	Msa1389140:CDS	40.0%
	AATCAGGTGGTAAAGCTTCA+GGG	+	tig0021212:96146-96165	Msa1389140:CDS	40.0%
	GCAGAGAATTACTGCAAGAA+AGG	+	tig0021212:96170-96189	Msa1389140:CDS	40.0%
!!	ATCCTACCAACGTTTGCTAT+GGG	-	tig0021212:96202-96221	None:intergenic	40.0%
	TAAACGGTGATGATCTTGTG+TGG	+	tig0021212:96359-96378	Msa1389140:CDS	40.0%
	AAACGGTGATGATCTTGTGT+GGG	+	tig0021212:96360-96379	Msa1389140:CDS	40.0%
	TTCAAAGCCAAGTGTAGTCA+TGG	-	tig0021212:96386-96405	None:intergenic	40.0%
	AGAATATGCAGAGCCTCTTA+AGG	+	tig0021212:96405-96424	Msa1389140:CDS	40.0%
	GAATATGCAGAGCCTCTTAA+GGG	+	tig0021212:96406-96425	Msa1389140:CDS	40.0%
	TTGTGAAGGTAACCCTTAAG+AGG	-	tig0021212:96421-96440	None:intergenic	40.0%
	AGGGTTACCTTCACAAGTAT+AGG	+	tig0021212:96425-96444	Msa1389140:CDS	40.0%
	GGGTTACCTTCACAAGTATA+GGG	+	tig0021212:96426-96445	Msa1389140:CDS	40.0%
	GAATCAGGTGGTAAAGCTTC+AGG	+	tig0021212:96145-96164	Msa1389140:CDS	45.0%
!!	TACCAACGTTTGCTATGGGA+AGG	-	tig0021212:96198-96217	None:intergenic	45.0%
	TTCCCATAGCAAACGTTGGT+AGG	+	tig0021212:96197-96216	Msa1389140:CDS	45.0%
!	GATCCTACCAACGTTTGCTA+TGG	-	tig0021212:96203-96222	None:intergenic	45.0%
	TTCGATATCTTTGCTCTCGC+CGG	-	tig0021212:96241-96260	None:intergenic	45.0%
	CTCCTTCCCATAGCAAACGT+TGG	+	tig0021212:96193-96212	Msa1389140:CDS	50.0%
	GGTAGTAGTAAGGAGCAGCA+AGG	+	tig0021212:96487-96506	Msa1389140:CDS	50.0%
	GTGTGGGCCATGACTACACT+TGG	+	tig0021212:96376-96395	Msa1389140:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
tig0021212	gene	96127	96537	96127	ID=Msa1389140;Name=Msa1389140
tig0021212	mRNA	96127	96537	96127	ID=Msa1389140-mRNA-1;Parent=Msa1389140;Name=Msa1389140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
tig0021212	exon	96127	96537	96127	ID=Msa1389140-mRNA-1:exon:11448;Parent=Msa1389140-mRNA-1
tig0021212	CDS	96127	96537	96127	ID=Msa1389140-mRNA-1:cds;Parent=Msa1389140-mRNA-1

Gene Sequence

>Msa1389140

ATGGCAGAATCAGATGATGAATCAGGTGGTAAAGCTTCAGGGAGCAGAGAATTACTGCAAGAAAGGCTCCTTCCCATAGCAAACGTTGGTAGGATCATGAAGAAAGCTTTACCGGCGAGAGCAAAGATATCGAAAGAAGCTAAAGAAACGATGCAAGAGTGTGTATCAGAGTTCATAAGCTTCATAACAGGTGAAGCATCTGAAAAGTGTCAGAAAGAGAAGAGGAAGACAATAAACGGTGATGATCTTGTGTGGGCCATGACTACACTTGGCTTTGAAGAATATGCAGAGCCTCTTAAGGGTTACCTTCACAAGTATAGGGAAATTGAAGGTGACAAGAATTTTTCTATGAATATGATTGGTAGTAGTAAGGAGCAGCAAGGTAGTATTGATCGATTCTTCCAAGGTTGA

Protein sequence

>Msa1389140

MAESDDESGGKASGSRELLQERLLPIANVGRIMKKALPARAKISKEAKETMQECVSEFISFITGEASEKCQKEKRKTINGDDLVWAMTTLGFEEYAEPLKGYLHKYREIEGDKNFSMNMIGSSKEQQGSIDRFFQG*