AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0175840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0175840 MtrunA17_Chr1g0197331 97.170 212 6 0 1 212 1 212 7.37e-153 422
Msa0175840 MtrunA17_Chr7g0265001 70.936 203 56 2 12 212 13 214 4.65e-103 296
Msa0175840 MtrunA17_Chr1g0180291 71.782 202 53 2 12 211 11 210 6.68e-101 291
Msa0175840 MtrunA17_Chr8g0384311 63.679 212 72 3 1 211 1 208 5.64e-92 268
Msa0175840 MtrunA17_Chr1g0159481 64.039 203 69 2 12 212 14 214 8.95e-91 265
Msa0175840 MtrunA17_Chr3g0130271 64.390 205 63 4 12 211 9 208 3.06e-88 258
Msa0175840 MtrunA17_Chr4g0055651 47.549 204 103 4 9 212 2 201 3.33e-54 172
Msa0175840 MtrunA17_Chr7g0250531 48.039 204 102 4 9 212 2 201 7.13e-54 171
Msa0175840 MtrunA17_Chr1g0182321 44.240 217 112 6 1 211 1 214 6.26e-52 166
Msa0175840 MtrunA17_Chr1g0212521 45.411 207 104 6 11 211 11 214 8.69e-52 166
Msa0175840 MtrunA17_Chr1g0182301 44.240 217 112 6 1 211 1 214 1.15e-51 166
Msa0175840 MtrunA17_Chr8g0335471 45.588 204 108 3 9 212 2 202 1.33e-51 165
Msa0175840 MtrunA17_Chr4g0030911 43.689 206 108 5 11 211 11 213 1.27e-50 163
Msa0175840 MtrunA17_Chr7g0263071 46.316 190 94 5 11 195 11 197 1.96e-48 157
Msa0175840 MtrunA17_Chr2g0276931 44.390 205 111 3 7 211 1 202 7.11e-48 155
Msa0175840 MtrunA17_Chr6g0458081 49.419 172 82 4 11 181 2 169 8.70e-48 155
Msa0175840 MtrunA17_Chr2g0327911 42.647 204 112 5 11 211 11 212 5.52e-47 154
Msa0175840 MtrunA17_Chr2g0283131 42.857 217 106 7 8 212 6 216 6.93e-46 151
Msa0175840 MtrunA17_Chr8g0349711 49.375 160 76 4 14 172 5 160 1.81e-45 150
Msa0175840 MtrunA17_Chr2g0315551 41.589 214 113 5 8 212 6 216 8.72e-45 148
Msa0175840 MtrunA17_Chr2g0290891 49.080 163 78 4 11 172 2 160 9.02e-45 148
Msa0175840 MtrunA17_Chr4g0046261 41.935 217 108 7 8 212 6 216 3.09e-44 147
Msa0175840 MtrunA17_Chr4g0040781 46.893 177 91 3 8 184 6 179 4.14e-44 146
Msa0175840 MtrunA17_Chr1g0189361 40.183 219 105 6 13 212 12 223 3.27e-43 144
Msa0175840 MtrunA17_Chr6g0482721 47.093 172 88 3 13 184 10 178 4.39e-43 144
Msa0175840 MtrunA17_Chr2g0319951 44.633 177 95 3 8 184 6 179 9.27e-43 143
Msa0175840 MtrunA17_Chr2g0325091 42.453 212 111 5 9 212 5 213 2.83e-42 142
Msa0175840 MtrunA17_Chr4g0032201 43.353 173 95 3 12 184 9 178 5.77e-42 141
Msa0175840 MtrunA17_Chr8g0381841 43.478 184 99 4 1 182 1 181 6.47e-42 141
Msa0175840 MtrunA17_Chr2g0276941 44.509 173 93 3 1 173 1 170 1.04e-41 140
Msa0175840 MtrunA17_Chr8g0349681 47.561 164 81 4 14 176 12 171 1.07e-41 148
Msa0175840 MtrunA17_Chr8g0349681 49.383 162 77 4 12 172 381 538 1.30e-41 148
Msa0175840 MtrunA17_Chr8g0349681 45.732 164 86 3 9 172 169 329 1.52e-39 142
Msa0175840 MtrunA17_Chr3g0123821 42.286 175 98 3 8 182 9 180 2.15e-41 140
Msa0175840 MtrunA17_Chr3g0112681 43.575 179 97 4 6 184 4 178 1.09e-40 138
Msa0175840 MtrunA17_Chr7g0234201 45.213 188 88 6 14 191 12 194 1.04e-39 135
Msa0175840 MtrunA17_Chr7g0231131 41.954 174 98 3 9 182 9 179 1.25e-39 135
Msa0175840 MtrunA17_Chr7g0214241 42.473 186 96 4 14 191 14 196 4.33e-39 134
Msa0175840 MtrunA17_Chr1g0174281 36.530 219 118 5 9 211 9 222 5.95e-39 134
Msa0175840 MtrunA17_Chr7g0275991 44.253 174 94 3 13 186 18 188 6.58e-39 134
Msa0175840 MtrunA17_Chr8g0392931 44.828 174 93 3 13 186 12 182 2.02e-37 129
Msa0175840 MtrunA17_Chr8g0351861 45.000 160 85 3 13 172 14 170 2.76e-37 129
Msa0175840 MtrunA17_Chr8g0346421 42.778 180 97 4 13 192 24 197 5.94e-37 129
Msa0175840 MtrunA17_Chr5g0433561 35.885 209 123 6 5 212 2 200 1.72e-36 126
Msa0175840 MtrunA17_Chr3g0109721 34.928 209 125 6 5 212 2 200 1.38e-35 124
Msa0175840 MtrunA17_Chr3g0123971 37.186 199 120 4 16 212 10 205 4.95e-35 123
Msa0175840 MtrunA17_Chr8g0390561 35.714 196 118 4 17 212 11 198 1.05e-34 122
Msa0175840 MtrunA17_Chr4g0055081 35.859 198 121 4 16 210 10 204 1.94e-34 121
Msa0175840 MtrunA17_Chr8g0382221 37.000 200 120 5 16 212 10 206 1.70e-33 119
Msa0175840 MtrunA17_Chr1g0199331 39.181 171 97 6 17 185 33 198 1.86e-32 116
Msa0175840 MtrunA17_Chr5g0400131 38.235 170 102 3 16 185 10 176 2.89e-32 116
Msa0175840 MtrunA17_Chr4g0034871 35.567 194 114 6 16 200 9 200 7.64e-28 104
Msa0175840 MtrunA17_Chr2g0331691 38.415 164 93 5 16 173 11 172 3.74e-26 100
Msa0175840 MtrunA17_Chr6g0466301 36.139 202 118 8 16 211 9 205 1.03e-25 99.0
Msa0175840 MtrunA17_Chr6g0465491 36.139 202 118 8 16 211 9 205 1.03e-25 99.0
Msa0175840 MtrunA17_Chr1g0201121 39.264 163 91 5 16 172 9 169 4.91e-25 97.4
Msa0175840 MtrunA17_Chr4g0004661 38.037 163 93 5 17 173 66 226 6.34e-25 98.2
Msa0175840 MtrunA17_Chr8g0383261 39.264 163 91 5 16 172 13 173 2.17e-24 95.5
Msa0175840 MtrunA17_Chr6g0486041 29.756 205 118 8 17 210 8 197 2.84e-21 87.4
Msa0175840 MtrunA17_Chr3g0138101 31.429 175 113 5 13 186 10 178 1.30e-20 85.9
Msa0175840 MtrunA17_Chr3g0138111 31.429 175 113 5 13 186 10 178 1.34e-20 85.9
Msa0175840 MtrunA17_Chr3g0138121 31.429 175 113 5 13 186 10 178 1.38e-20 85.9
Msa0175840 MtrunA17_Chr4g0037021 30.256 195 116 8 16 199 7 192 8.61e-19 80.5
Msa0175840 MtrunA17_Chr2g0325101 29.545 176 108 6 16 180 7 177 1.38e-18 80.1
Msa0175840 MtrunA17_Chr3g0117931 28.718 195 119 7 17 200 10 195 4.86e-18 79.0
Msa0175840 MtrunA17_Chr5g0405791 28.977 176 109 6 16 180 7 177 5.91e-17 75.9
Msa0175840 MtrunA17_Chr8g0371501 28.571 175 109 6 17 180 8 177 1.10e-16 75.1
Msa0175840 MtrunA17_Chr6g0465471 33.136 169 102 4 48 210 22 185 3.86e-14 68.6
Msa0175840 MtrunA17_Chr6g0466161 33.136 169 102 4 48 210 22 185 4.15e-14 68.2
Msa0175840 MtrunA17_Chr1g0178291 34.682 173 96 6 10 182 426 581 2.04e-13 68.6
Msa0175840 MtrunA17_Chr4g0046211 30.081 123 82 3 17 139 8 126 9.96e-13 63.2
Msa0175840 MtrunA17_Chr6g0466431 46.269 67 35 1 48 114 31 96 1.39e-12 61.6
Msa0175840 MtrunA17_Chr6g0465611 46.269 67 35 1 48 114 31 96 1.39e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0175840 AT5G03530.2 70.561 214 57 3 1 212 1 210 1.95e-107 307
Msa0175840 AT5G03530.1 70.561 214 57 3 1 212 1 210 1.95e-107 307
Msa0175840 AT1G43890.3 74.211 190 45 2 1 190 1 186 5.73e-99 286
Msa0175840 AT1G43890.1 74.211 190 45 2 1 190 1 186 5.73e-99 286
Msa0175840 AT1G43890.2 74.211 190 45 2 1 190 1 186 5.73e-99 286
Msa0175840 AT3G09910.3 66.509 212 63 3 1 212 1 204 1.96e-90 264
Msa0175840 AT3G09910.1 66.509 212 63 3 1 212 1 204 1.96e-90 264
Msa0175840 AT3G09910.2 66.509 212 63 3 1 212 1 204 1.96e-90 264
Msa0175840 AT3G09910.5 69.274 179 51 2 1 179 1 175 3.08e-80 238
Msa0175840 AT3G09910.4 69.274 179 51 2 1 179 1 175 3.08e-80 238
Msa0175840 AT1G02130.1 48.039 204 103 3 9 212 2 202 5.81e-55 174
Msa0175840 AT5G47200.1 47.549 204 103 4 9 212 2 201 7.20e-53 169
Msa0175840 AT4G17530.1 47.549 204 103 4 9 212 2 201 2.20e-52 167
Msa0175840 AT5G03520.1 44.700 217 112 5 1 212 1 214 3.36e-52 167
Msa0175840 AT5G59840.1 45.411 207 104 6 11 211 11 214 4.50e-52 167
Msa0175840 AT3G09900.1 44.749 219 111 6 1 212 1 216 5.21e-52 167
Msa0175840 AT3G53610.2 44.928 207 105 6 11 211 11 214 4.40e-51 165
Msa0175840 AT3G53610.3 44.928 207 105 6 11 211 11 214 4.40e-51 165
Msa0175840 AT3G53610.1 44.928 207 105 6 11 211 11 214 4.40e-51 165
Msa0175840 AT3G46060.2 44.444 207 106 6 11 211 11 214 1.71e-50 163
Msa0175840 AT3G46060.3 44.444 207 106 6 11 211 11 214 1.71e-50 163
Msa0175840 AT3G46060.1 44.444 207 106 6 11 211 11 214 1.71e-50 163
Msa0175840 AT3G11730.1 46.860 207 95 5 12 211 5 203 6.08e-50 161
Msa0175840 AT4G35860.1 49.419 172 82 4 11 181 2 169 5.18e-49 159
Msa0175840 AT4G17160.1 44.231 208 104 6 11 211 2 204 2.73e-47 154
Msa0175840 AT4G17170.1 43.192 213 105 6 11 211 2 210 1.60e-46 153
Msa0175840 AT5G45750.1 42.723 213 111 5 8 212 6 215 1.51e-45 150
Msa0175840 AT1G18200.1 47.647 170 86 3 13 182 11 177 1.06e-44 149
Msa0175840 AT4G18800.1 41.232 211 115 4 8 212 6 213 1.37e-44 148
Msa0175840 AT5G60860.1 46.893 177 91 3 8 184 6 179 3.91e-44 147
Msa0175840 AT5G47520.1 39.815 216 107 5 13 212 12 220 1.01e-43 146
Msa0175840 AT1G16920.1 41.905 210 111 5 11 212 9 215 1.33e-43 145
Msa0175840 AT3G15060.1 45.763 177 93 3 8 184 6 179 6.43e-43 144
Msa0175840 AT1G73640.1 45.977 174 91 3 13 186 11 181 6.66e-43 144
Msa0175840 AT4G39990.1 45.509 167 88 3 13 179 15 178 7.41e-43 144
Msa0175840 AT2G33870.1 47.126 174 89 3 11 184 9 179 1.99e-42 142
Msa0175840 AT1G28550.1 47.126 174 89 3 11 184 9 179 2.55e-42 142
Msa0175840 AT4G18430.1 44.068 177 96 3 8 184 6 179 2.89e-42 142
Msa0175840 AT1G05810.1 47.305 167 85 3 13 179 10 173 3.89e-42 142
Msa0175840 AT3G12160.1 38.532 218 112 5 9 211 9 219 9.16e-42 141
Msa0175840 AT1G05810.2 47.305 167 85 3 13 179 56 219 9.37e-42 142
Msa0175840 AT2G43130.1 41.038 212 105 6 13 212 10 213 4.96e-41 139
Msa0175840 AT5G65270.1 42.353 170 95 3 13 182 15 181 3.19e-40 137
Msa0175840 AT5G59150.1 41.379 174 99 3 11 184 8 178 3.20e-40 137
Msa0175840 AT3G07410.1 42.654 211 106 7 13 212 10 216 3.68e-40 137
Msa0175840 AT1G07410.1 37.500 208 120 4 12 212 9 213 4.07e-40 137
Msa0175840 AT2G31680.1 46.584 161 83 3 13 173 10 167 5.26e-40 136
Msa0175840 AT3G46830.1 41.477 176 100 3 9 184 6 178 5.31e-40 136
Msa0175840 AT3G46830.2 41.477 176 100 3 9 184 6 178 5.31e-40 136
Msa0175840 AT1G06400.1 43.017 179 99 3 8 186 6 181 6.41e-40 136
Msa0175840 AT5G47960.1 42.045 176 99 3 9 184 9 181 1.37e-39 135
Msa0175840 AT5G03520.2 42.775 173 92 4 45 212 34 204 3.54e-39 134
Msa0175840 AT1G09630.1 42.045 176 99 3 9 184 6 178 4.03e-39 134
Msa0175840 AT1G01200.1 44.099 161 86 4 13 172 26 183 1.48e-35 125
Msa0175840 AT4G35860.2 49.593 123 60 2 59 181 3 123 4.64e-35 122
Msa0175840 AT5G45130.1 36.979 192 112 6 1 191 1 184 5.67e-33 118
Msa0175840 AT3G54840.1 40.000 165 93 5 17 179 35 195 1.02e-32 117
Msa0175840 AT4G39890.1 37.278 169 104 2 16 184 10 176 1.96e-32 117
Msa0175840 AT3G54840.3 39.634 164 93 5 18 179 2 161 2.49e-32 115
Msa0175840 AT2G22290.1 34.975 203 120 5 16 212 10 206 3.70e-32 116
Msa0175840 AT4G19640.1 35.484 186 116 4 11 195 6 188 5.40e-32 115
Msa0175840 AT3G54840.2 41.509 159 87 5 17 173 35 189 7.12e-32 115
Msa0175840 AT2G44610.1 35.000 200 122 4 16 210 10 206 7.71e-32 115
Msa0175840 AT5G10260.1 34.500 200 124 5 16 211 10 206 1.17e-31 114
Msa0175840 AT5G45130.2 36.782 174 106 4 19 191 22 192 3.84e-31 113
Msa0175840 AT3G18820.1 37.113 194 111 6 16 200 9 200 8.25e-29 107
Msa0175840 AT5G64990.1 39.752 161 86 4 16 172 8 161 1.12e-28 107
Msa0175840 AT1G22740.1 36.364 209 103 8 16 210 9 201 1.02e-27 104
Msa0175840 AT1G49300.2 38.037 163 93 5 16 172 9 169 2.65e-26 100
Msa0175840 AT1G49300.1 38.037 163 93 5 16 172 9 169 2.65e-26 100
Msa0175840 AT1G52280.1 39.634 164 91 5 16 173 9 170 4.28e-26 100
Msa0175840 AT4G09720.1 40.491 163 89 5 16 172 9 169 5.02e-26 100
Msa0175840 AT5G64990.2 38.095 168 86 5 16 172 8 168 6.23e-26 100
Msa0175840 AT4G09720.4 39.286 168 89 6 16 172 9 174 9.49e-26 99.8
Msa0175840 AT4G09720.3 37.931 174 89 6 16 172 9 180 4.27e-25 98.2
Msa0175840 AT5G59150.2 39.344 122 72 2 63 184 8 127 1.14e-24 95.5
Msa0175840 AT3G16100.1 34.536 194 116 6 16 200 9 200 3.88e-24 95.5
Msa0175840 AT5G39620.1 36.047 172 100 6 16 180 6 174 2.98e-22 90.5
Msa0175840 AT4G19640.2 35.971 139 86 3 11 149 6 141 3.96e-22 88.6
Msa0175840 AT2G21880.1 36.196 163 96 5 16 172 10 170 1.07e-21 89.0
Msa0175840 AT4G19640.3 37.500 128 77 3 17 144 12 136 4.38e-21 87.0
Msa0175840 AT2G44690.1 32.240 183 109 5 17 188 20 198 2.82e-20 85.1
Msa0175840 AT5G55190.1 29.592 196 122 7 13 198 10 199 5.37e-20 84.7
Msa0175840 AT5G20010.1 30.769 182 111 6 13 185 10 185 5.37e-20 84.7
Msa0175840 AT5G20020.1 30.769 182 111 6 13 185 10 185 6.62e-20 84.3
Msa0175840 AT4G28950.1 27.941 204 124 8 16 210 7 196 1.97e-19 83.2
Msa0175840 AT4G09720.6 41.221 131 70 4 48 172 6 135 4.82e-19 81.3
Msa0175840 AT4G09720.5 41.221 131 70 4 48 172 6 135 4.82e-19 81.3
Msa0175840 AT4G09720.2 41.221 131 70 4 48 172 6 135 4.82e-19 81.3
Msa0175840 AT5G55080.1 30.723 166 107 5 15 179 13 171 7.02e-19 81.6
Msa0175840 AT4G35020.1 30.726 179 108 6 17 184 8 181 1.54e-18 80.5
Msa0175840 AT4G35020.2 30.726 179 108 6 17 184 8 181 1.54e-18 80.5
Msa0175840 AT4G35020.3 30.726 179 108 6 17 184 8 181 1.54e-18 80.5
Msa0175840 AT3G48040.1 28.729 181 113 7 16 185 9 184 1.10e-17 78.2
Msa0175840 AT3G51300.1 28.571 175 109 6 17 180 8 177 4.24e-17 76.6
Msa0175840 AT1G20090.1 28.000 175 110 6 17 180 7 176 4.72e-17 76.3
Msa0175840 AT1G75840.1 28.000 175 110 6 17 180 8 177 6.36e-17 75.9
Msa0175840 AT5G45970.1 28.736 174 108 6 17 179 8 176 1.78e-16 75.1
Msa0175840 AT2G17800.2 28.571 175 109 6 17 180 8 177 2.05e-16 74.7
Msa0175840 AT2G17800.1 28.571 175 109 6 17 180 8 177 2.05e-16 74.7
Msa0175840 AT5G62880.1 29.762 168 103 6 16 172 9 172 2.56e-16 74.7
Msa0175840 AT4G35950.1 28.000 175 110 6 17 180 8 177 6.21e-16 73.6
Msa0175840 AT2G21880.4 34.228 149 89 5 48 190 16 161 1.09e-15 72.4
Msa0175840 AT2G21880.3 34.228 149 89 5 48 190 16 161 1.09e-15 72.4
Msa0175840 AT5G46025.1 41.053 95 53 3 86 179 14 106 5.49e-15 68.9
Msa0175840 AT5G62880.2 29.651 172 102 7 17 177 10 173 2.37e-14 68.9
Msa0175840 AT3G48040.2 28.571 161 100 6 17 166 10 166 4.39e-14 68.2
Msa0175840 AT5G64813.1 30.986 142 72 4 17 134 24 163 2.29e-12 65.5
Msa0175840 AT5G64813.3 30.986 142 72 4 17 134 24 163 2.29e-12 65.5
Msa0175840 AT5G64813.2 30.986 142 72 4 17 134 24 163 2.29e-12 65.5
Msa0175840 AT3G51290.2 25.503 149 96 5 43 180 634 778 7.75e-11 61.2
Msa0175840 AT3G21700.3 25.000 180 127 3 15 189 106 282 8.07e-11 60.5

Find 67 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATTGAAGCTTACTATT+TGG 0.159285 1_4:-61975280 Msa0175840:CDS
CATAATTCTGTTATAAGTTT+TGG 0.196246 1_4:-61971728 Msa0175840:three_prime_UTR
CTTTAAAGATCTCTCATTAA+AGG 0.198626 1_4:-61972678 Msa0175840:intron
CAAAATTCTTGTTGGCAATA+AGG 0.238391 1_4:-61973001 Msa0175840:CDS
AGCCATATAATTTAGTATTT+AGG 0.251158 1_4:-61971785 Msa0175840:three_prime_UTR
GTGCCAAGTTTGCGCGAAAA+AGG 0.252534 1_4:-61972028 Msa0175840:CDS
AAGATTGAAGCTTACTATTT+GGG 0.304127 1_4:-61975279 Msa0175840:CDS
GTTAGGATCGATTTGAGATT+TGG 0.312457 1_4:-61971653 Msa0175840:three_prime_UTR
CGTCCAGGCGGTTGAGAGTA+TGG 0.315243 1_4:+61976289 None:intergenic
AAGAACAGCAACCACCTCTT+TGG 0.332793 1_4:+61971957 None:intergenic
GATGACCTTTCTCCCACTAT+TGG 0.342365 1_4:-61976003 Msa0175840:intron
AGAGATCTTTAAAGCATTGC+TGG 0.346216 1_4:+61972687 None:intergenic
CATTTCACCCTTTATGATCA+TGG 0.351737 1_4:-61976206 Msa0175840:five_prime_UTR
AATTGATTTACAGATATTAG+AGG 0.361028 1_4:-61972050 Msa0175840:intron
TCATGGCCAAATGATTCATT+AGG 0.367086 1_4:-61976189 Msa0175840:five_prime_UTR
CACCTAAATACTAAATTATA+TGG 0.367837 1_4:+61971783 None:intergenic
CATATGATGTACAATATTGT+AGG 0.371624 1_4:+61971700 None:intergenic
GATTGCATCAAAATTCTTGT+TGG 0.374427 1_4:-61973009 Msa0175840:CDS
TCATTTGGCCATGATCATAA+AGG 0.377860 1_4:+61976198 None:intergenic
ATGGGTTCTTCTTCAAAAGT+TGG 0.381996 1_4:-61976129 Msa0175840:CDS
ACCAATCCTAATGAATCATT+TGG 0.383302 1_4:+61976183 None:intergenic
GGCGCACATGGAATCGTTCT+TGG 0.394093 1_4:-61973411 Msa0175840:intron
ATGATTCATTAGGATTGGTT+TGG 0.394356 1_4:-61976179 Msa0175840:five_prime_UTR
GATCGATTTGAGATTTGGTT+TGG 0.395154 1_4:-61971648 Msa0175840:three_prime_UTR
CGATCCTAACACATTCAAAC+TGG 0.403854 1_4:+61971666 None:intergenic
TGTTGGCAATAAGGTGGATA+AGG 0.416713 1_4:-61972992 Msa0175840:intron
GCAGCTGGACAAGAGAGGTT+TGG 0.417511 1_4:-61973459 Msa0175840:intron
GTGTCACAGATCACATAGTT+TGG 0.418049 1_4:-61971853 Msa0175840:three_prime_UTR
GCCAAATGATTCATTAGGAT+TGG 0.425604 1_4:-61976184 Msa0175840:five_prime_UTR
GTCTCCAGTTTGAATGTGTT+AGG 0.428973 1_4:-61971670 Msa0175840:three_prime_UTR
TTGATTGGTGATTCTGGTGT+TGG 0.435841 1_4:-61976072 Msa0175840:CDS
AGAACAGCAACCACCTCTTT+GGG 0.437718 1_4:+61971958 None:intergenic
ATTTGAGATTTGGTTTGGTT+AGG 0.442917 1_4:-61971643 Msa0175840:three_prime_UTR
ATTGCTCTCACAATATCTCT+TGG 0.466083 1_4:-61971808 Msa0175840:three_prime_UTR
ACCAAGCAAGCTGATTTCCT+TGG 0.468615 1_4:-61976313 None:intergenic
GAAGGTAAGGTTGTCCGTCC+AGG 0.480146 1_4:+61976274 None:intergenic
GAGAACCAATAGTGGGAGAA+AGG 0.483210 1_4:+61975998 None:intergenic
CATTTGGCCATGATCATAAA+GGG 0.488183 1_4:+61976199 None:intergenic
TTACAAACCTGGTGGGAACA+TGG 0.492228 1_4:-61973065 Msa0175840:CDS
AGAAAGAAAGCAAAGTAAGA+AGG 0.492318 1_4:+61976229 None:intergenic
GAAGAGAAGAGAACCAATAG+TGG 0.493822 1_4:+61975990 None:intergenic
GGAACATGGGCAAAAGAGGT+TGG 0.518611 1_4:-61973051 Msa0175840:CDS
AAAATGAGACAAAGAAGGTA+AGG 0.523000 1_4:+61976261 None:intergenic
GGTGGGAACATGGGCAAAAG+AGG 0.525668 1_4:-61973055 Msa0175840:CDS
AGAGGGTATGGCTCTTGCAC+AGG 0.538120 1_4:-61972759 Msa0175840:CDS
CAGATACCACGGTTACTAGC+TGG 0.542142 1_4:-61971894 Msa0175840:three_prime_UTR
TGTAAGCAAAGAAGAGGGTA+TGG 0.544429 1_4:-61972771 Msa0175840:CDS
TACAAACCTGGTGGGAACAT+GGG 0.559570 1_4:-61973064 Msa0175840:CDS
GGACCATACTCTCAACCGCC+TGG 0.560156 1_4:-61976292 Msa0175840:five_prime_UTR
AAGCTTACTATTTGGGACAC+AGG 0.560298 1_4:-61975272 Msa0175840:intron
ACAAGAAAATGAGACAAAGA+AGG 0.568313 1_4:+61976256 None:intergenic
AATTCTTGTTGGCAATAAGG+TGG 0.571818 1_4:-61972998 Msa0175840:CDS
TCTTATTACAGAGGCGCACA+TGG 0.580596 1_4:-61973423 Msa0175840:CDS
GCGAGAGACATTTACAAACC+TGG 0.589818 1_4:-61973076 Msa0175840:CDS
AAGAGAAGAGAACCAATAGT+GGG 0.590629 1_4:+61975991 None:intergenic
TACGAGACATCCCAAAGAGG+TGG 0.593872 1_4:-61971968 Msa0175840:CDS
AAGAGCTGTAAGCAAAGAAG+AGG 0.595756 1_4:-61972777 Msa0175840:CDS
CATACTCTCAACCGCCTGGA+CGG 0.607094 1_4:-61976288 Msa0175840:five_prime_UTR
AGAGCTGTAAGCAAAGAAGA+GGG 0.635800 1_4:-61972776 Msa0175840:CDS
GCGGTTGAGAGTATGGTCCA+AGG 0.658089 1_4:+61976296 None:intergenic
ATTTAGTGTATGATGTAACA+AGG 0.660740 1_4:-61973098 Msa0175840:intron
GGTAAGGTTGTCCGTCCAGG+CGG 0.673872 1_4:+61976277 None:intergenic
GTGATATGCTCTTATTACAG+AGG 0.674573 1_4:-61973432 Msa0175840:CDS
GAGACATTTACAAACCTGGT+GGG 0.674869 1_4:-61973072 Msa0175840:CDS
CAAAGACCAGCTAGTAACCG+TGG 0.689529 1_4:+61971888 None:intergenic
AGAGACATTTACAAACCTGG+TGG 0.692793 1_4:-61973073 Msa0175840:CDS
CTTTAACAGAACAGATACCA+CGG 0.705753 1_4:-61971905 Msa0175840:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATGTTTTCAAAATTT+TGG + chr1_4:61972123-61972142 None:intergenic 10.0%
!! AAATAACATGAAAAATAATT+TGG - chr1_4:61972139-61972158 Msa0175840:intron 10.0%
!! AAATTCAAACAAAAAAAAAA+AGG + chr1_4:61976228-61976247 None:intergenic 10.0%
!! AACTAGAAAGAAAAAATAAA+AGG + chr1_4:61971862-61971881 None:intergenic 15.0%
!!! TTTAACTTCTCTATTATAAT+AGG - chr1_4:61972371-61972390 Msa0175840:intron 15.0%
!! TTAACTTCTCTATTATAATA+GGG - chr1_4:61972372-61972391 Msa0175840:intron 15.0%
!!! TAAAACACATTATATTTTTG+TGG + chr1_4:61972794-61972813 None:intergenic 15.0%
!! AATTATATTTCCAAATATGA+TGG - chr1_4:61973388-61973407 Msa0175840:intron 15.0%
!! TATCTGTAAATCAATTTATA+TGG + chr1_4:61975769-61975788 None:intergenic 15.0%
!!! TTTATTTTTTTGTCAAGTAA+GGG + chr1_4:61976305-61976324 None:intergenic 15.0%
!!! ATTTATTTTTTTGTCAAGTA+AGG + chr1_4:61976306-61976325 None:intergenic 15.0%
!! AAAAAGAATACTAACAACAT+GGG - chr1_4:61971676-61971695 Msa0175840:three_prime_UTR 20.0%
!!! TAACTATGATTACTCTTTTA+AGG - chr1_4:61971723-61971742 Msa0175840:three_prime_UTR 20.0%
!!! TTTCTTGATTCATTTTGATT+TGG - chr1_4:61971928-61971947 Msa0175840:three_prime_UTR 20.0%
!! AAGTATCATAAAATATGACA+AGG + chr1_4:61971957-61971976 None:intergenic 20.0%
!!! ATGTTTTCAAAATTTTGGAA+AGG + chr1_4:61972118-61972137 None:intergenic 20.0%
!!! TTTTACATAACATACTGAAA+TGG - chr1_4:61972283-61972302 Msa0175840:intron 20.0%
!!! TTTTTGATAAGTTGATATCT+AGG - chr1_4:61972323-61972342 Msa0175840:intron 20.0%
!! TTAAAAACTATGAATGAAGA+AGG + chr1_4:61972356-61972375 None:intergenic 20.0%
!!! AAAATCAAGCATTTTACAAT+TGG - chr1_4:61972515-61972534 Msa0175840:intron 20.0%
!!! TATATTTTTGTGGTTGTTTA+TGG + chr1_4:61972784-61972803 None:intergenic 20.0%
!! TGATGAATAAATTCATTCAT+TGG - chr1_4:61972981-61973000 Msa0175840:intron 20.0%
!! TGTAACATGAAAAATATGTA+AGG - chr1_4:61973684-61973703 Msa0175840:intron 20.0%
!! TTCTATCAGTTAAAATATGA+TGG + chr1_4:61974093-61974112 None:intergenic 20.0%
!!! AAACTACTTGATAACTTATT+TGG - chr1_4:61974941-61974960 Msa0175840:intron 20.0%
!!! CTTGGTTTTATTATATGATT+GGG - chr1_4:61975589-61975608 Msa0175840:intron 20.0%
!! TAAAAAACTTGGACAAATAA+AGG - chr1_4:61975645-61975664 Msa0175840:intron 20.0%
!!! GTTAAAATTTTAGTGGAAAA+AGG + chr1_4:61975715-61975734 None:intergenic 20.0%
!!! AATTGATTTACAGATATTAG+AGG - chr1_4:61975773-61975792 Msa0175840:intron 20.0%
!! AGCCATATAATTTAGTATTT+AGG - chr1_4:61976038-61976057 Msa0175840:CDS 20.0%
!! CACCTAAATACTAAATTATA+TGG + chr1_4:61976043-61976062 None:intergenic 20.0%
!!! CATAATTCTGTTATAAGTTT+TGG - chr1_4:61976095-61976114 Msa0175840:CDS 20.0%
!! ACATATTTACCGTAAAAAAA+AGG + chr1_4:61976277-61976296 None:intergenic 20.0%
! GAAAAAGAATACTAACAACA+TGG - chr1_4:61971675-61971694 Msa0175840:three_prime_UTR 25.0%
!!! TCTTTTAAGGTTTTGTTGAT+TGG - chr1_4:61971736-61971755 Msa0175840:three_prime_UTR 25.0%
!!! TATGTTTTCAAGATTTTGTG+AGG - chr1_4:61972085-61972104 Msa0175840:intron 25.0%
!!! ATGTTTTCAAGATTTTGTGA+GGG - chr1_4:61972086-61972105 Msa0175840:intron 25.0%
!!! GGTATTTGTCTTTGAAATTT+TGG - chr1_4:61972414-61972433 Msa0175840:intron 25.0%
! AAAGATTGAAGCTTACTATT+TGG - chr1_4:61972543-61972562 Msa0175840:intron 25.0%
! AAGATTGAAGCTTACTATTT+GGG - chr1_4:61972544-61972563 Msa0175840:intron 25.0%
! AAAACCAATGAAATTCTGTT+TGG - chr1_4:61972656-61972675 Msa0175840:intron 25.0%
!!! TGGTTTTGTAATTATGATTC+AGG + chr1_4:61972764-61972783 None:intergenic 25.0%
! AATGAACTATCCATCATATT+TGG + chr1_4:61973401-61973420 None:intergenic 25.0%
! CAATATTCTCCTTATTGTAA+GGG + chr1_4:61973451-61973470 None:intergenic 25.0%
! ACAATATTCTCCTTATTGTA+AGG + chr1_4:61973452-61973471 None:intergenic 25.0%
! TGAGTAGTATAACATGAAAT+TGG - chr1_4:61973752-61973771 Msa0175840:intron 25.0%
! GAGTAGTATAACATGAAATT+GGG - chr1_4:61973753-61973772 Msa0175840:intron 25.0%
! AATATATGTTCTACTTCTCA+AGG - chr1_4:61974018-61974037 Msa0175840:intron 25.0%
! TATTATAATCTAACTGCAAC+AGG + chr1_4:61974330-61974349 None:intergenic 25.0%
! ATTTGTAGCAAAATTATCCA+GGG + chr1_4:61974637-61974656 None:intergenic 25.0%
! TATTTGTAGCAAAATTATCC+AGG + chr1_4:61974638-61974657 None:intergenic 25.0%
! ATTTAGTGTATGATGTAACA+AGG - chr1_4:61974725-61974744 Msa0175840:intron 25.0%
! AAGGTAAATATTATGGTCTA+TGG - chr1_4:61974850-61974869 Msa0175840:intron 25.0%
! AAATATTATGGTCTATGGTA+TGG - chr1_4:61974855-61974874 Msa0175840:intron 25.0%
! TAAGTTATCAAGTAGTTTGA+GGG + chr1_4:61974939-61974958 None:intergenic 25.0%
! ATAAGTTATCAAGTAGTTTG+AGG + chr1_4:61974940-61974959 None:intergenic 25.0%
!!! TGTATTGTTTTTCACTTTAC+AGG - chr1_4:61975016-61975035 Msa0175840:intron 25.0%
!! CTTTAAAGATCTCTCATTAA+AGG - chr1_4:61975145-61975164 Msa0175840:intron 25.0%
! AGAACATGCATATATGAATT+TGG + chr1_4:61975381-61975400 None:intergenic 25.0%
! GATGGTTAAAAAATAGAACT+CGG - chr1_4:61975448-61975467 Msa0175840:intron 25.0%
! TTAAAAAATAGAACTCGGTA+TGG - chr1_4:61975453-61975472 Msa0175840:intron 25.0%
! TAACGTGAATGTTTCAAAAA+CGG - chr1_4:61975505-61975524 Msa0175840:intron 25.0%
! CCAATCATATAATAAAACCA+AGG + chr1_4:61975591-61975610 None:intergenic 25.0%
!!! CCTTGGTTTTATTATATGAT+TGG - chr1_4:61975588-61975607 Msa0175840:intron 25.0%
!!! TTTTTTAGATCAGCTTATTG+TGG + chr1_4:61975632-61975651 None:intergenic 25.0%
! ATAAGCTGATCTAAAAAACT+TGG - chr1_4:61975634-61975653 Msa0175840:intron 25.0%
!! GAAAGCTGTTAAAATTTTAG+TGG + chr1_4:61975722-61975741 None:intergenic 25.0%
! CATATGATGTACAATATTGT+AGG + chr1_4:61976126-61976145 None:intergenic 25.0%
AAAATGAGACAAAGAAGGTA+AGG + chr1_4:61971565-61971584 None:intergenic 30.0%
ACAAGAAAATGAGACAAAGA+AGG + chr1_4:61971570-61971589 None:intergenic 30.0%
AGAAAGAAAGCAAAGTAAGA+AGG + chr1_4:61971597-61971616 None:intergenic 30.0%
ACCAATCCTAATGAATCATT+TGG + chr1_4:61971643-61971662 None:intergenic 30.0%
!! ATGATTCATTAGGATTGGTT+TGG - chr1_4:61971644-61971663 Msa0175840:three_prime_UTR 30.0%
!! ATAAGTTGATATCTAGGCTT+TGG - chr1_4:61972329-61972348 Msa0175840:intron 30.0%
GGTATTCTTAAATCATTCAC+AGG - chr1_4:61972482-61972501 Msa0175840:intron 30.0%
GAAAACGTGATCTAATAACT+AGG + chr1_4:61972587-61972606 None:intergenic 30.0%
ATTTCTCGAGTAATGCTATA+AGG - chr1_4:61972625-61972644 Msa0175840:intron 30.0%
GTAACCAAACAGAATTTCAT+TGG + chr1_4:61972663-61972682 None:intergenic 30.0%
TACTATATATACCCGTCAAA+TGG - chr1_4:61972680-61972699 Msa0175840:CDS 30.0%
! AGCTTTAGCTATATGTCATA+TGG - chr1_4:61972813-61972832 Msa0175840:intron 30.0%
CGAATTCTATTCTAATTAGC+CGG + chr1_4:61972894-61972913 None:intergenic 30.0%
CGGCTAATTAGAATAGAATT+CGG - chr1_4:61972892-61972911 Msa0175840:intron 30.0%
AAATTCATTCATTGGAACCT+TGG - chr1_4:61972989-61973008 Msa0175840:intron 30.0%
! AGATGGGAATTTGGATTTTA+GGG - chr1_4:61973289-61973308 Msa0175840:intron 30.0%
!! ATCTAGAGAAGTGGATTTTT+CGG + chr1_4:61973321-61973340 None:intergenic 30.0%
CAAATTACATTCTACTTTGC+TGG + chr1_4:61973352-61973371 None:intergenic 30.0%
!!! ACCTTTTAGTTTTATGTGCT+GGG + chr1_4:61973502-61973521 None:intergenic 30.0%
!!! AACCTTTTAGTTTTATGTGC+TGG + chr1_4:61973503-61973522 None:intergenic 30.0%
! TCTCTGTTTGAAATTTCAGT+GGG + chr1_4:61973571-61973590 None:intergenic 30.0%
! ATCTCTGTTTGAAATTTCAG+TGG + chr1_4:61973572-61973591 None:intergenic 30.0%
TTTCAAACAGAGATTACAGA+AGG - chr1_4:61973578-61973597 Msa0175840:intron 30.0%
ATGTTATACTACTCATCATC+CGG + chr1_4:61973747-61973766 None:intergenic 30.0%
AAATTGGGAAACACTAAATC+AGG - chr1_4:61973768-61973787 Msa0175840:intron 30.0%
! TGGATGATTACTTCTTTTGT+TGG - chr1_4:61973870-61973889 Msa0175840:intron 30.0%
!!! ATTACTTCTTTTGTTGGTTC+TGG - chr1_4:61973876-61973895 Msa0175840:intron 30.0%
!!! TTACTTCTTTTGTTGGTTCT+GGG - chr1_4:61973877-61973896 Msa0175840:intron 30.0%
TAGAACATATATTAGACTCG+AGG + chr1_4:61974011-61974030 None:intergenic 30.0%
AACATGAAATCTTGTAGTGT+TGG - chr1_4:61974304-61974323 Msa0175840:intron 30.0%
!!! ATTTAGTTTTTTTTGGCAGC+TGG - chr1_4:61974349-61974368 Msa0175840:intron 30.0%
!! TCTTTTGTTATAGCAATGCT+TGG - chr1_4:61974598-61974617 Msa0175840:intron 30.0%
TTTGTAGCAAAATTATCCAG+GGG + chr1_4:61974636-61974655 None:intergenic 30.0%
AATATTAGATGTCAGCACTA+AGG + chr1_4:61974686-61974705 None:intergenic 30.0%
GATTGCATCAAAATTCTTGT+TGG - chr1_4:61974814-61974833 Msa0175840:intron 30.0%
CAAAATTCTTGTTGGCAATA+AGG - chr1_4:61974822-61974841 Msa0175840:intron 30.0%
GGTGGATAAGGTAAATATTA+TGG - chr1_4:61974843-61974862 Msa0175840:intron 30.0%
AAGTTATCAAGTAGTTTGAG+GGG + chr1_4:61974938-61974957 None:intergenic 30.0%
TGTTGGTATTCAAATGTGAA+TGG - chr1_4:61975206-61975225 Msa0175840:intron 30.0%
CAACATTTGATACATAAGAG+AGG - chr1_4:61975256-61975275 Msa0175840:intron 30.0%
AAAACAGAACTATGCGAAAT+TGG + chr1_4:61975338-61975357 None:intergenic 30.0%
! TATGAATTTGGAATCAAGAG+TGG + chr1_4:61975369-61975388 None:intergenic 30.0%
AATAAAACCAAGGCATAAGA+GGG + chr1_4:61975581-61975600 None:intergenic 30.0%
TAATAAAACCAAGGCATAAG+AGG + chr1_4:61975582-61975601 None:intergenic 30.0%
! TTTTAGTGGAAAAAGGTGAT+TGG + chr1_4:61975708-61975727 None:intergenic 30.0%
!! ATTTGAGATTTGGTTTGGTT+AGG - chr1_4:61976180-61976199 Msa0175840:five_prime_UTR 30.0%
! GTAAAAAAAAGGTACAACAG+GGG + chr1_4:61976266-61976285 None:intergenic 30.0%
! CGTAAAAAAAAGGTACAACA+GGG + chr1_4:61976267-61976286 None:intergenic 30.0%
!!! CCTGTTGTACCTTTTTTTTA+CGG - chr1_4:61976265-61976284 Msa0175840:five_prime_UTR 30.0%
!!! ATTTTTTTGTCAAGTAAGGG+AGG + chr1_4:61976302-61976321 None:intergenic 30.0%
CATTTCACCCTTTATGATCA+TGG - chr1_4:61971617-61971636 Msa0175840:three_prime_UTR 35.0%
CATTTGGCCATGATCATAAA+GGG + chr1_4:61971627-61971646 None:intergenic 35.0%
TCATTTGGCCATGATCATAA+AGG + chr1_4:61971628-61971647 None:intergenic 35.0%
TCATGGCCAAATGATTCATT+AGG - chr1_4:61971634-61971653 Msa0175840:three_prime_UTR 35.0%
GCCAAATGATTCATTAGGAT+TGG - chr1_4:61971639-61971658 Msa0175840:three_prime_UTR 35.0%
ATGGGTTCTTCTTCAAAAGT+TGG - chr1_4:61971694-61971713 Msa0175840:three_prime_UTR 35.0%
!! GTTTTGTTGATTGGTGATTC+TGG - chr1_4:61971745-61971764 Msa0175840:three_prime_UTR 35.0%
AAGAGAAGAGAACCAATAGT+GGG + chr1_4:61971835-61971854 None:intergenic 35.0%
ACTAGTATTAAGCATGTGCT+CGG + chr1_4:61972221-61972240 None:intergenic 35.0%
GGTAGTTTATCTGAATTCTG+TGG - chr1_4:61972393-61972412 Msa0175840:intron 35.0%
TTTGTTGCATACTGAGTTGT+TGG - chr1_4:61972461-61972480 Msa0175840:intron 35.0%
CAGACGATATAAAGAAGAGA+AGG + chr1_4:61972708-61972727 None:intergenic 35.0%
CTTCTCTTCTTTATATCGTC+TGG - chr1_4:61972706-61972725 Msa0175840:CDS 35.0%
ACAGAATTCTGAGTTCTCTA+GGG - chr1_4:61972860-61972879 Msa0175840:intron 35.0%
AAGACAGAGAATGTATGTGT+AGG - chr1_4:61972918-61972937 Msa0175840:intron 35.0%
AGACAGAGAATGTATGTGTA+GGG - chr1_4:61972919-61972938 Msa0175840:intron 35.0%
!!! GGAGAAATAGAGTTTTAGAG+AGG - chr1_4:61972940-61972959 Msa0175840:intron 35.0%
GAATATTCTCCTTAGTGCAA+GGG + chr1_4:61973033-61973052 None:intergenic 35.0%
AGAATATTCTCCTTAGTGCA+AGG + chr1_4:61973034-61973053 None:intergenic 35.0%
!!! GTAGTTTTATGCACTGAGTT+AGG + chr1_4:61973077-61973096 None:intergenic 35.0%
ACTCAGTGCATAAAACTACA+TGG - chr1_4:61973078-61973097 Msa0175840:CDS 35.0%
ATGACAAGGATGTGCTTAAA+GGG + chr1_4:61973155-61973174 None:intergenic 35.0%
TCATTTCATCCATGATGACA+AGG + chr1_4:61973169-61973188 None:intergenic 35.0%
TGGATGAAATGAGTGAACTT+AGG - chr1_4:61973177-61973196 Msa0175840:intron 35.0%
ATCCAAATTCCCATCTCAAT+TGG + chr1_4:61973285-61973304 None:intergenic 35.0%
! GAGATGGGAATTTGGATTTT+AGG - chr1_4:61973288-61973307 Msa0175840:intron 35.0%
AAAATCCACTTCTCTAGATC+AGG - chr1_4:61973322-61973341 Msa0175840:intron 35.0%
!!! TTTAGTTTTATGTGCTGGGA+TGG + chr1_4:61973498-61973517 None:intergenic 35.0%
TCCCAGCACATAAAACTAAA+AGG - chr1_4:61973498-61973517 Msa0175840:intron 35.0%
TTTATAGCATTCGTTGTCAC+CGG - chr1_4:61973725-61973744 Msa0175840:intron 35.0%
!!! AATCAGGTTGAGGTTTTCAT+TGG - chr1_4:61973784-61973803 Msa0175840:intron 35.0%
! TTCATTGGCTATGTTGGATT+CGG - chr1_4:61973799-61973818 Msa0175840:intron 35.0%
ACCATGAGGTCTATTCTATT+GGG + chr1_4:61973937-61973956 None:intergenic 35.0%
AACCATGAGGTCTATTCTAT+TGG + chr1_4:61973938-61973957 None:intergenic 35.0%
!!! TTTGGTGCCAAATCCTTTTA+AGG + chr1_4:61973965-61973984 None:intergenic 35.0%
TTACTTGATGTTGACGTCTT+TGG + chr1_4:61973983-61974002 None:intergenic 35.0%
! TGTAGCTTTTGCCAAAATCA+CGG + chr1_4:61974250-61974269 None:intergenic 35.0%
GTGATATGCTCTTATTACAG+AGG - chr1_4:61974391-61974410 Msa0175840:intron 35.0%
TTGGATGTATTAATATCCCC+TGG - chr1_4:61974617-61974636 Msa0175840:intron 35.0%
AATTCTTGTTGGCAATAAGG+TGG - chr1_4:61974825-61974844 Msa0175840:intron 35.0%
CTTGCATTCATTACTTACTC+AGG - chr1_4:61974879-61974898 Msa0175840:intron 35.0%
GGGAGAAAACATAATCTGTA+TGG + chr1_4:61974918-61974937 None:intergenic 35.0%
!! AGAGATCTTTAAAGCATTGC+TGG + chr1_4:61975139-61975158 None:intergenic 35.0%
! TATGCGAAATTGGTGATCAA+AGG + chr1_4:61975328-61975347 None:intergenic 35.0%
GGTGATTGGATCATACATTA+GGG + chr1_4:61975694-61975713 None:intergenic 35.0%
AGGTGATTGGATCATACATT+AGG + chr1_4:61975695-61975714 None:intergenic 35.0%
CTTTAACAGAACAGATACCA+CGG - chr1_4:61975918-61975937 Msa0175840:intron 35.0%
ATTGCTCTCACAATATCTCT+TGG - chr1_4:61976015-61976034 Msa0175840:CDS 35.0%
!! GTTAGGATCGATTTGAGATT+TGG - chr1_4:61976170-61976189 Msa0175840:five_prime_UTR 35.0%
!! GATCGATTTGAGATTTGGTT+TGG - chr1_4:61976175-61976194 Msa0175840:five_prime_UTR 35.0%
! CCGTAAAAAAAAGGTACAAC+AGG + chr1_4:61976268-61976287 None:intergenic 35.0%
! TTGATTGGTGATTCTGGTGT+TGG - chr1_4:61971751-61971770 Msa0175840:three_prime_UTR 40.0%
GAAGAGAAGAGAACCAATAG+TGG + chr1_4:61971836-61971855 None:intergenic 40.0%
ATGTCCATACGTAGAGTTGT+TGG - chr1_4:61971978-61971997 Msa0175840:CDS 40.0%
TAGACCAACAACTCTACGTA+TGG + chr1_4:61971985-61972004 None:intergenic 40.0%
! ACGTAGAGTTGTTGGTCTAA+TGG - chr1_4:61971986-61972005 Msa0175840:CDS 40.0%
! AAGCTTACTATTTGGGACAC+AGG - chr1_4:61972551-61972570 Msa0175840:intron 40.0%
! TTACTATTTGGGACACAGGT+TGG - chr1_4:61972555-61972574 Msa0175840:intron 40.0%
AAGAGAAGGAACCATTTGAC+GGG + chr1_4:61972694-61972713 None:intergenic 40.0%
GAAGAGAAGGAACCATTTGA+CGG + chr1_4:61972695-61972714 None:intergenic 40.0%
GACAGAATTCTGAGTTCTCT+AGG - chr1_4:61972859-61972878 Msa0175840:intron 40.0%
TGGAACCTTGGTCACAATAT+AGG - chr1_4:61973001-61973020 Msa0175840:CDS 40.0%
TCATGCCTATATTGTGACCA+AGG + chr1_4:61973009-61973028 None:intergenic 40.0%
TGACAAGGATGTGCTTAAAG+GGG + chr1_4:61973154-61973173 None:intergenic 40.0%
GATGACAAGGATGTGCTTAA+AGG + chr1_4:61973156-61973175 None:intergenic 40.0%
TAAGCACATCCTTGTCATCA+TGG - chr1_4:61973157-61973176 Msa0175840:intron 40.0%
ATCAACCTGGTGCTTAATCA+TGG + chr1_4:61973253-61973272 None:intergenic 40.0%
TGCCAATTGAGATGGGAATT+TGG - chr1_4:61973280-61973299 Msa0175840:intron 40.0%
GAATTCCTGATCTAGAGAAG+TGG + chr1_4:61973330-61973349 None:intergenic 40.0%
AATAGCGTGCCCTTACAATA+AGG - chr1_4:61973439-61973458 Msa0175840:CDS 40.0%
ACAGAGATTACAGAAGGCTT+TGG - chr1_4:61973584-61973603 Msa0175840:intron 40.0%
! GGAAACACTAAATCAGGTTG+AGG - chr1_4:61973774-61973793 Msa0175840:intron 40.0%
!! GAGGTTTTCATTGGCTATGT+TGG - chr1_4:61973793-61973812 Msa0175840:intron 40.0%
AATCAGATTTCTCTGTCCAC+TGG - chr1_4:61973843-61973862 Msa0175840:intron 40.0%
AGTAATCATCCATGAACCAG+TGG + chr1_4:61973862-61973881 None:intergenic 40.0%
AGGTCTATTCTATTGGGTCA+AGG + chr1_4:61973931-61973950 None:intergenic 40.0%
ACCCAATAGAATAGACCTCA+TGG - chr1_4:61973933-61973952 Msa0175840:intron 40.0%
GTTGCGTCCTTAAAAGGATT+TGG - chr1_4:61973955-61973974 Msa0175840:intron 40.0%
!!! TGGTTTTGAGTTCTGTTCAC+AGG + chr1_4:61974073-61974092 None:intergenic 40.0%
AGTTTGTCAGAACCACAGTT+TGG - chr1_4:61974222-61974241 Msa0175840:intron 40.0%
AAAATCACGGTACCAAACTG+TGG + chr1_4:61974237-61974256 None:intergenic 40.0%
! ACAGTTTGGTACCGTGATTT+TGG - chr1_4:61974236-61974255 Msa0175840:intron 40.0%
CAAAAGCTACACTTTCGACA+TGG - chr1_4:61974259-61974278 Msa0175840:intron 40.0%
ATTTCAAGGGCTTGAATGTG+GGG + chr1_4:61974563-61974582 None:intergenic 40.0%
GATTTCAAGGGCTTGAATGT+GGG + chr1_4:61974564-61974583 None:intergenic 40.0%
TGATTTCAAGGGCTTGAATG+TGG + chr1_4:61974565-61974584 None:intergenic 40.0%
TTGTGCGTGCTTGATTTCAA+GGG + chr1_4:61974576-61974595 None:intergenic 40.0%
AGAGACATTTACAAACCTGG+TGG - chr1_4:61974750-61974769 Msa0175840:intron 40.0%
GAGACATTTACAAACCTGGT+GGG - chr1_4:61974751-61974770 Msa0175840:intron 40.0%
TGTTGGCAATAAGGTGGATA+AGG - chr1_4:61974831-61974850 Msa0175840:intron 40.0%
AAGAGCTGTAAGCAAAGAAG+AGG - chr1_4:61975046-61975065 Msa0175840:intron 40.0%
AGAGCTGTAAGCAAAGAAGA+GGG - chr1_4:61975047-61975066 Msa0175840:intron 40.0%
TGTAAGCAAAGAAGAGGGTA+TGG - chr1_4:61975052-61975071 Msa0175840:intron 40.0%
! TGGTTTTTGCACTACACTCT+AGG + chr1_4:61975106-61975125 None:intergenic 40.0%
! ATTTGGAATCAAGAGTGGTG+TGG + chr1_4:61975364-61975383 None:intergenic 40.0%
!! GAAGAACAGCAGATTTTAGC+TGG + chr1_4:61975747-61975766 None:intergenic 40.0%
! TTTTACGAGACATCCCAAAG+AGG - chr1_4:61975852-61975871 Msa0175840:intron 40.0%
GTGTCACAGATCACATAGTT+TGG - chr1_4:61975970-61975989 Msa0175840:intron 40.0%
GTCTCCAGTTTGAATGTGTT+AGG - chr1_4:61976153-61976172 Msa0175840:five_prime_UTR 40.0%
CGATCCTAACACATTCAAAC+TGG + chr1_4:61976160-61976179 None:intergenic 40.0%
! AAAAAAAGGTACAACAGGGG+TGG + chr1_4:61976263-61976282 None:intergenic 40.0%
GATGACCTTTCTCCCACTAT+TGG - chr1_4:61971820-61971839 Msa0175840:three_prime_UTR 45.0%
! GAGAACCAATAGTGGGAGAA+AGG + chr1_4:61971828-61971847 None:intergenic 45.0%
GTATTAAGCATGTGCTCGGT+TGG + chr1_4:61972217-61972236 None:intergenic 45.0%
!! AATTCTGAGTTCTCTAGGGC+AGG - chr1_4:61972864-61972883 Msa0175840:intron 45.0%
AACCTGGTGCTTAATCATGG+CGG + chr1_4:61973250-61973269 None:intergenic 45.0%
TGCTCTGATGCCAATTGAGA+TGG - chr1_4:61973272-61973291 Msa0175840:intron 45.0%
GCTCTGATGCCAATTGAGAT+GGG - chr1_4:61973273-61973292 Msa0175840:intron 45.0%
GGGCACGCTATTACTAACAA+GGG + chr1_4:61973431-61973450 None:intergenic 45.0%
AGGGCACGCTATTACTAACA+AGG + chr1_4:61973432-61973451 None:intergenic 45.0%
!! TTTCTCTGTCCACTGGTTCA+TGG - chr1_4:61973850-61973869 Msa0175840:intron 45.0%
!! CTTTTAAGGACGCAACCATG+AGG + chr1_4:61973951-61973970 None:intergenic 45.0%
CTCATGGTTGCGTCCTTAAA+AGG - chr1_4:61973949-61973968 Msa0175840:intron 45.0%
TGAGTTCTGTTCACAGGCTT+GGG + chr1_4:61974067-61974086 None:intergenic 45.0%
TTGAGTTCTGTTCACAGGCT+TGG + chr1_4:61974068-61974087 None:intergenic 45.0%
CCAAACATGCACTAAGCTGA+TGG + chr1_4:61974187-61974206 None:intergenic 45.0%
CCATCAGCTTAGTGCATGTT+TGG - chr1_4:61974184-61974203 Msa0175840:intron 45.0%
GCATGTTTGGATTGACAGTG+AGG - chr1_4:61974197-61974216 Msa0175840:intron 45.0%
! TCTTATTACAGAGGCGCACA+TGG - chr1_4:61974400-61974419 Msa0175840:intron 45.0%
TTTCAAGGGCTTGAATGTGG+GGG + chr1_4:61974562-61974581 None:intergenic 45.0%
CTTGTGCGTGCTTGATTTCA+AGG + chr1_4:61974577-61974596 None:intergenic 45.0%
GCGAGAGACATTTACAAACC+TGG - chr1_4:61974747-61974766 Msa0175840:intron 45.0%
TTACAAACCTGGTGGGAACA+TGG - chr1_4:61974758-61974777 Msa0175840:intron 45.0%
TACAAACCTGGTGGGAACAT+GGG - chr1_4:61974759-61974778 Msa0175840:intron 45.0%
! GCATTGCTGGACATTTTCTC+TGG + chr1_4:61975126-61975145 None:intergenic 45.0%
CATCTGTAGTAGTCGAGTGT+TGG - chr1_4:61975189-61975208 Msa0175840:intron 45.0%
!! GGTGATCAAAGGTGTCACTT+AGG + chr1_4:61975317-61975336 None:intergenic 45.0%
! CCATCTGAGCATTGTCATTG+CGG + chr1_4:61975433-61975452 None:intergenic 45.0%
TTCATCACCCTCTTATGCCT+TGG - chr1_4:61975571-61975590 Msa0175840:intron 45.0%
TGCCAAGTTTGCGCGAAAAA+GGG - chr1_4:61975796-61975815 Msa0175840:intron 45.0%
AAGTTTGCGCGAAAAAGGGT+CGG - chr1_4:61975800-61975819 Msa0175840:intron 45.0%
AGAACAGCAACCACCTCTTT+GGG + chr1_4:61975868-61975887 None:intergenic 45.0%
AAGAACAGCAACCACCTCTT+TGG + chr1_4:61975869-61975888 None:intergenic 45.0%
!!! TTATAATATTTAGTTTTTTT+TGG - chr1_4:61974342-61974361 Msa0175840:intron 5.0%
!! GTTCTCTAGGGCAGGAATTC+CGG - chr1_4:61972872-61972891 Msa0175840:intron 50.0%
AGGCATGAACCCTTGCACTA+AGG - chr1_4:61973021-61973040 Msa0175840:CDS 50.0%
!! GCTTAATCATGGCGGATGGA+AGG + chr1_4:61973242-61973261 None:intergenic 50.0%
! TGGTGCTTAATCATGGCGGA+TGG + chr1_4:61973246-61973265 None:intergenic 50.0%
ATCCGCCATGATTAAGCACC+AGG - chr1_4:61973245-61973264 Msa0175840:intron 50.0%
! TTGGCATCAGAGCATCAACC+TGG + chr1_4:61973266-61973285 None:intergenic 50.0%
! CTGGGATGGAAAGTGTGTAG+TGG + chr1_4:61973484-61973503 None:intergenic 50.0%
TGGGTTTGTGTGCTCACAAG+AGG + chr1_4:61974048-61974067 None:intergenic 50.0%
! TTTTGGCAGCTGGACAAGAG+AGG - chr1_4:61974359-61974378 Msa0175840:intron 50.0%
GGAACATGGGCAAAAGAGGT+TGG - chr1_4:61974772-61974791 Msa0175840:intron 50.0%
CCGCAATGACAATGCTCAGA+TGG - chr1_4:61975430-61975449 Msa0175840:intron 50.0%
GTGCCAAGTTTGCGCGAAAA+AGG - chr1_4:61975795-61975814 Msa0175840:intron 50.0%
!! GACCCTTTTTCGCGCAAACT+TGG + chr1_4:61975801-61975820 None:intergenic 50.0%
TACGAGACATCCCAAAGAGG+TGG - chr1_4:61975855-61975874 Msa0175840:intron 50.0%
CAGATACCACGGTTACTAGC+TGG - chr1_4:61975929-61975948 Msa0175840:intron 50.0%
CAAAGACCAGCTAGTAACCG+TGG + chr1_4:61975938-61975957 None:intergenic 50.0%
! GCGGTTGAGAGTATGGTCCA+AGG + chr1_4:61971530-61971549 None:intergenic 55.0%
CATACTCTCAACCGCCTGGA+CGG - chr1_4:61971535-61971554 Msa0175840:three_prime_UTR 55.0%
GAAGGTAAGGTTGTCCGTCC+AGG + chr1_4:61971552-61971571 None:intergenic 55.0%
GAAGGATTCACCCCTAGGCA+TGG + chr1_4:61973224-61973243 None:intergenic 55.0%
GGATGGAAGGATTCACCCCT+AGG + chr1_4:61973229-61973248 None:intergenic 55.0%
GCAGCTGGACAAGAGAGGTT+TGG - chr1_4:61974364-61974383 Msa0175840:intron 55.0%
GGCGCACATGGAATCGTTCT+TGG - chr1_4:61974412-61974431 Msa0175840:intron 55.0%
! CTTTTGCCCATGTTCCCACC+AGG + chr1_4:61974768-61974787 None:intergenic 55.0%
GGTGGGAACATGGGCAAAAG+AGG - chr1_4:61974768-61974787 Msa0175840:intron 55.0%
! AGAGGGTATGGCTCTTGCAC+AGG - chr1_4:61975064-61975083 Msa0175840:intron 55.0%
GGACCATACTCTCAACCGCC+TGG - chr1_4:61971531-61971550 Msa0175840:three_prime_UTR 60.0%
CGTCCAGGCGGTTGAGAGTA+TGG + chr1_4:61971537-61971556 None:intergenic 60.0%
GGTAAGGTTGTCCGTCCAGG+CGG + chr1_4:61971549-61971568 None:intergenic 60.0%
AGACTCCGAGTGCCATGCCT+AGG - chr1_4:61973209-61973228 Msa0175840:intron 60.0%
GACTCCGAGTGCCATGCCTA+GGG - chr1_4:61973210-61973229 Msa0175840:intron 60.0%
ACTCCGAGTGCCATGCCTAG+GGG - chr1_4:61973211-61973230 Msa0175840:intron 60.0%
TCACCCCTAGGCATGGCACT+CGG + chr1_4:61973217-61973236 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 61971519 61976326 61971519 ID=Msa0175840;Name=Msa0175840
chr1_4 mRNA 61971519 61976326 61971519 ID=Msa0175840-mRNA-1;Parent=Msa0175840;Name=Msa0175840-mRNA-1;_AED=0.12;_eAED=0.12;_QI=175|1|1|1|1|1|6|436|212
chr1_4 exon 61971519 61972059 61971519 ID=Msa0175840-mRNA-1:exon:19870;Parent=Msa0175840-mRNA-1
chr1_4 exon 61972679 61972807 61972679 ID=Msa0175840-mRNA-1:exon:19869;Parent=Msa0175840-mRNA-1
chr1_4 exon 61972993 61973114 61972993 ID=Msa0175840-mRNA-1:exon:19868;Parent=Msa0175840-mRNA-1
chr1_4 exon 61973412 61973477 61973412 ID=Msa0175840-mRNA-1:exon:19867;Parent=Msa0175840-mRNA-1
chr1_4 exon 61975273 61975341 61975273 ID=Msa0175840-mRNA-1:exon:19866;Parent=Msa0175840-mRNA-1
chr1_4 exon 61976004 61976326 61976004 ID=Msa0175840-mRNA-1:exon:19865;Parent=Msa0175840-mRNA-1
chr1_4 five_prime_UTR 61976152 61976326 61976152 ID=Msa0175840-mRNA-1:five_prime_utr;Parent=Msa0175840-mRNA-1
chr1_4 CDS 61976004 61976151 61976004 ID=Msa0175840-mRNA-1:cds;Parent=Msa0175840-mRNA-1
chr1_4 CDS 61975273 61975341 61975273 ID=Msa0175840-mRNA-1:cds;Parent=Msa0175840-mRNA-1
chr1_4 CDS 61973412 61973477 61973412 ID=Msa0175840-mRNA-1:cds;Parent=Msa0175840-mRNA-1
chr1_4 CDS 61972993 61973114 61972993 ID=Msa0175840-mRNA-1:cds;Parent=Msa0175840-mRNA-1
chr1_4 CDS 61972679 61972807 61972679 ID=Msa0175840-mRNA-1:cds;Parent=Msa0175840-mRNA-1
chr1_4 CDS 61971955 61972059 61971955 ID=Msa0175840-mRNA-1:cds;Parent=Msa0175840-mRNA-1
chr1_4 three_prime_UTR 61971519 61971954 61971519 ID=Msa0175840-mRNA-1:three_prime_utr;Parent=Msa0175840-mRNA-1
Gene Sequence

>Msa0175840

ATGGGTTCTTCTTCAAAAGTTGGAAATAGTAACTATGATTACTCTTTTAAGGTTTTGTTGATTGGTGATTCTGGTGTTGGCAAGAGTAGTCTTCTTCTCAGCTTTATCTCTAATGCTAACTCACTTGATGACCTTTCTCCCACTATTGGAGTAGATTTCAAAATCAAGCATTTTACAATTGGTGATAAAAGATTGAAGCTTACTATTTGGGACACAGCTGGACAAGAGAGGTTTGGAACAGTGATATGCTCTTATTACAGAGGCGCACATGGAATCGTTCTTGTGTATGATGTAACAAGGCGAGAGACATTTACAAACCTGGTGGGAACATGGGCAAAAGAGGTTGGACTTTACTGCACTAATCAAGATTGCATCAAAATTCTTGTTGGCAATAAGGTGGATAAGGAGAGTGAAAGAGCTGTAAGCAAAGAAGAGGGTATGGCTCTTGCACAGGAACATAGATGCTTGTTCCTAGAGTGTAGTGCAAAAACCAGAGAAAATGTCCAGCAATGCTTTAAAGATCTCTCATTAAAGATATTAGAGGTGCCAAGTTTGCGCGAAAAAGGGTCGGTAGAAGTGAAGAGACAAAAGCAAAAGCGCTTTTACGAGACATCCCAAAGAGGTGGTTGCTGTTCTTAG
Protein sequence

>Msa0175840

MGSSSKVGNSNYDYSFKVLLIGDSGVGKSSLLLSFISNANSLDDLSPTIGVDFKIKHFTIGDKRLKLTIWDTAGQERFGTVICSYYRGAHGIVLVYDVTRRETFTNLVGTWAKEVGLYCTNQDCIKILVGNKVDKESERAVSKEEGMALAQEHRCLFLECSAKTRENVQQCFKDLSLKILEVPSLREKGSVEVKRQKQKRFYETSQRGGCCS*