AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0732880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732880 MtrunA17_Chr5g0427841 93.407 91 6 0 1 91 1 91 1.04e-54 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732880 AT2G38465.1 57.971 69 29 0 19 87 13 81 8.99e-22 81.6

Find 15 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCACAACAAAACTTCTTT+TGG 0.285814 5_1:+45562986 Msa0732880:CDS
ATATATATTTCATCTCTATA+AGG 0.409482 5_1:+45562910 Msa0732880:five_prime_UTR
CATTAATTTAATTAACTATG+AGG 0.415967 5_1:-45563318 None:intergenic
CTGCTGAACATATCAAGAAT+AGG 0.429170 5_1:+45563286 Msa0732880:CDS
AAAAGCCTTCTTTGTGGGTC+AGG 0.435149 5_1:+45563072 Msa0732880:CDS
TATGCAAAAGCCTTCTTTGT+GGG 0.462187 5_1:+45563067 Msa0732880:CDS
AAAAGTAGCTATGGAAGAGA+TGG 0.471774 5_1:+45562937 Msa0732880:exon
GTTTATTTATCTACAAAAGA+AGG 0.478248 5_1:+45562830 Msa0732880:five_prime_UTR
TAAGGCAATAAAAGTAGCTA+TGG 0.485068 5_1:+45562928 Msa0732880:exon
ATCTACAAAAGAAGGACAAA+TGG 0.493293 5_1:+45562838 Msa0732880:five_prime_UTR
ATATGCAAAAGCCTTCTTTG+TGG 0.519860 5_1:+45563066 Msa0732880:CDS
AATTACCTGACCCACAAAGA+AGG 0.535666 5_1:-45563077 None:intergenic
AAAGTAGCTATGGAAGAGAT+GGG 0.580832 5_1:+45562938 Msa0732880:exon
GGCTTCAAAGAAACAAGTTG+AGG 0.622095 5_1:+45563254 Msa0732880:CDS
AGCAAGTGAAGCTGAACTTG+AGG 0.634101 5_1:+45563233 Msa0732880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATTTCATCTCTATA+AGG + chr5_1:45562910-45562929 Msa0732880:five_prime_UTR 15.0%
!! CATTAATTTAATTAACTATG+AGG - chr5_1:45563321-45563340 None:intergenic 15.0%
!! GTTTATTTATCTACAAAAGA+AGG + chr5_1:45562830-45562849 Msa0732880:five_prime_UTR 20.0%
!!! TGTTATTTTGAAATTGATTG+AGG + chr5_1:45563155-45563174 Msa0732880:intron 20.0%
!!! AAAGAAGTTTTGTTGTGATT+TGG - chr5_1:45562986-45563005 None:intergenic 25.0%
!! AATCACAACAAAACTTCTTT+TGG + chr5_1:45562986-45563005 Msa0732880:CDS 25.0%
! AGATAAGTAGTAGCTAAAAA+TGG - chr5_1:45563126-45563145 None:intergenic 25.0%
! ATTGTGGTGTGAAAAAAAAT+AGG + chr5_1:45563179-45563198 Msa0732880:intron 25.0%
! TATAACTGCAAAAAATGAGA+AGG + chr5_1:45563209-45563228 Msa0732880:CDS 25.0%
ATCTACAAAAGAAGGACAAA+TGG + chr5_1:45562838-45562857 Msa0732880:five_prime_UTR 30.0%
TAAGGCAATAAAAGTAGCTA+TGG + chr5_1:45562928-45562947 Msa0732880:exon 30.0%
TAGTAGCTAAAAATGGAAAG+GGG - chr5_1:45563119-45563138 None:intergenic 30.0%
GTAGTAGCTAAAAATGGAAA+GGG - chr5_1:45563120-45563139 None:intergenic 30.0%
AGTAGTAGCTAAAAATGGAA+AGG - chr5_1:45563121-45563140 None:intergenic 30.0%
TGAAATTGATTGAGGAATTG+TGG + chr5_1:45563163-45563182 Msa0732880:intron 30.0%
AAAAGTAGCTATGGAAGAGA+TGG + chr5_1:45562937-45562956 Msa0732880:exon 35.0%
AAAGTAGCTATGGAAGAGAT+GGG + chr5_1:45562938-45562957 Msa0732880:exon 35.0%
ATATGCAAAAGCCTTCTTTG+TGG + chr5_1:45563066-45563085 Msa0732880:CDS 35.0%
TATGCAAAAGCCTTCTTTGT+GGG + chr5_1:45563067-45563086 Msa0732880:CDS 35.0%
!! AAATGGAAAGGGGTTGTTTT+TGG - chr5_1:45563109-45563128 None:intergenic 35.0%
CTGCTGAACATATCAAGAAT+AGG + chr5_1:45563286-45563305 Msa0732880:CDS 35.0%
AATTACCTGACCCACAAAGA+AGG - chr5_1:45563080-45563099 None:intergenic 40.0%
GGCTTCAAAGAAACAAGTTG+AGG + chr5_1:45563254-45563273 Msa0732880:CDS 40.0%
AAAAGCCTTCTTTGTGGGTC+AGG + chr5_1:45563072-45563091 Msa0732880:CDS 45.0%
AGCAAGTGAAGCTGAACTTG+AGG + chr5_1:45563233-45563252 Msa0732880:CDS 45.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 45562817 45563415 45562817 ID=Msa0732880;Name=Msa0732880
chr5_1 mRNA 45562817 45563415 45562817 ID=Msa0732880-mRNA-1;Parent=Msa0732880;Name=Msa0732880-mRNA-1;_AED=0.00;_eAED=0.00;_QI=130|1|1|1|1|1|2|86|91
chr5_1 exon 45562817 45563093 45562817 ID=Msa0732880-mRNA-1:exon:12999;Parent=Msa0732880-mRNA-1
chr5_1 exon 45563201 45563415 45563201 ID=Msa0732880-mRNA-1:exon:13000;Parent=Msa0732880-mRNA-1
chr5_1 five_prime_UTR 45562817 45562946 45562817 ID=Msa0732880-mRNA-1:five_prime_utr;Parent=Msa0732880-mRNA-1
chr5_1 CDS 45562947 45563093 45562947 ID=Msa0732880-mRNA-1:cds;Parent=Msa0732880-mRNA-1
chr5_1 CDS 45563201 45563329 45563201 ID=Msa0732880-mRNA-1:cds;Parent=Msa0732880-mRNA-1
chr5_1 three_prime_UTR 45563330 45563415 45563330 ID=Msa0732880-mRNA-1:three_prime_utr;Parent=Msa0732880-mRNA-1
Gene Sequence

>Msa0732880

ATGGAAGAGATGGGTTCTTCATCTACAACATCTTTACCAAATCACAACAAAACTTCTTTTGGTACTCGTTTGAAGATCAGTTGCTTATCTTTTGTTGTTTCTATACAAGAAACTTTTAGATATGCAAAAGCCTTCTTTGTGGGTCAGGCAAAGACTATAACTGCAAAAAATGAGAAGGAAGCAAGTGAAGCTGAACTTGAGGCTTCAAAGAAACAAGTTGAGGCTGCTGATGCTGCTGAACATATCAAGAATAGGCTCAATGATCCTCATAGTTAA

Protein sequence

>Msa0732880

MEEMGSSSTTSLPNHNKTSFGTRLKISCLSFVVSIQETFRYAKAFFVGQAKTITAKNEKEASEAELEASKKQVEAADAAEHIKNRLNDPHS*