Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1405810 | KEH40763.1 | 94.444 | 72 | 4 | 0 | 1 | 72 | 1 | 72 | 7.95e-41 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1405810 | A0A072VH50 | 94.444 | 72 | 4 | 0 | 1 | 72 | 1 | 72 | 3.80e-41 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0013290 | Msa1405810 | 0.924392 | 7.493980e-90 | -8.615850e-47 |
| Msa0032580 | Msa1405810 | 0.808293 | 3.325877e-50 | -8.615850e-47 |
| Msa0032610 | Msa1405810 | 0.809889 | 1.513397e-50 | -8.615850e-47 |
| Msa0085360 | Msa1405810 | -0.800593 | 1.339868e-48 | -8.615850e-47 |
| Msa0106520 | Msa1405810 | 0.998823 | 5.582662e-278 | -8.615850e-47 |
| Msa0115390 | Msa1405810 | 0.818520 | 1.877748e-52 | -8.615850e-47 |
| Msa0623080 | Msa1405810 | 0.801391 | 9.206837e-49 | -8.615850e-47 |
| Msa0172780 | Msa1405810 | 0.825171 | 5.420643e-54 | -8.615850e-47 |
| Msa0172970 | Msa1405810 | 0.809004 | 2.343891e-50 | -8.615850e-47 |
| Msa0173990 | Msa1405810 | 0.800089 | 1.697490e-48 | -8.615850e-47 |
| Msa0177700 | Msa1405810 | -0.802787 | 4.752491e-49 | -8.615850e-47 |
| Msa0429290 | Msa1405810 | 0.802873 | 4.562740e-49 | -8.615850e-47 |
| Msa0996170 | Msa1405810 | 0.809689 | 1.671062e-50 | -8.615850e-47 |
| Msa0519220 | Msa1405810 | 0.804574 | 2.023683e-49 | -8.615850e-47 |
| Msa0874570 | Msa1405810 | 0.803558 | 3.291205e-49 | -8.615850e-47 |
| Msa0909000 | Msa1405810 | -0.800203 | 1.608810e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1405810 | MtrunA17_Chr1g0162551 | 94.444 | 72 | 4 | 0 | 1 | 72 | 1 | 72 | 7.31e-45 | 138 |
| Msa1405810 | MtrunA17_Chr1g0162491 | 77.778 | 72 | 11 | 1 | 1 | 72 | 1 | 67 | 1.27e-34 | 112 |
| Msa1405810 | MtrunA17_Chr4g0034081 | 52.000 | 75 | 30 | 3 | 1 | 71 | 1 | 73 | 8.39e-20 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTTGGATTTGGAAAATT+TGG | 0.091946 | tig0023172:-52254 | None:intergenic |
| CTCTTCTTCTAAACCATCTT+TGG | 0.198131 | tig0023172:-52269 | None:intergenic |
| TTCTAAACCATCTTTGGATT+TGG | 0.216462 | tig0023172:-52263 | None:intergenic |
| GTCAACAATATGTTTGCTTA+TGG | 0.258869 | tig0023172:+52360 | Msa1405810:CDS |
| TTAGAAGAAGAGAAGGAAGT+TGG | 0.379164 | tig0023172:+52280 | Msa1405810:CDS |
| TAGAACTTTCTCTTTGAGCT+TGG | 0.434544 | tig0023172:-52221 | None:intergenic |
| AGTTGGAAGGGAAGCTCAAA+AGG | 0.457096 | tig0023172:+52297 | Msa1405810:CDS |
| AGAAGAGAAGGAAGTTGGAA+GGG | 0.471300 | tig0023172:+52285 | Msa1405810:CDS |
| AAGAAGAGAAGGAAGTTGGA+AGG | 0.492250 | tig0023172:+52284 | Msa1405810:CDS |
| AGATGGTTTAGAAGAAGAGA+AGG | 0.510389 | tig0023172:+52273 | Msa1405810:CDS |
| ACATATTGTTGACTCAGATA+AGG | 0.525899 | tig0023172:-52350 | None:intergenic |
| TGTCGGCCATGGTAGAGGTA+TGG | 0.538180 | tig0023172:+52185 | Msa1405810:CDS |
| GCCATGGTAGAGGTATGGAT+TGG | 0.607798 | tig0023172:+52190 | Msa1405810:CDS |
| ACCAATCCATACCTCTACCA+TGG | 0.656584 | tig0023172:-52191 | None:intergenic |
| TGGAAGGGAAGCTCAAAAGG+AGG | 0.704527 | tig0023172:+52300 | Msa1405810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGGAAAATTTGGTTTTGT+TGG | - | tig0023172:52248-52267 | None:intergenic | 25.0% |
| ! | ATCTTTGGATTTGGAAAATT+TGG | - | tig0023172:52257-52276 | None:intergenic | 25.0% |
| !! | AAATTTTCCAAATCCAAAGA+TGG | + | tig0023172:52256-52275 | Msa1405810:CDS | 25.0% |
| TTCTAAACCATCTTTGGATT+TGG | - | tig0023172:52266-52285 | None:intergenic | 30.0% | |
| !! | ACATATTGTTGACTCAGATA+AGG | - | tig0023172:52353-52372 | None:intergenic | 30.0% |
| GTCAACAATATGTTTGCTTA+TGG | + | tig0023172:52360-52379 | Msa1405810:CDS | 30.0% | |
| TAGAACTTTCTCTTTGAGCT+TGG | - | tig0023172:52224-52243 | None:intergenic | 35.0% | |
| CTCTTCTTCTAAACCATCTT+TGG | - | tig0023172:52272-52291 | None:intergenic | 35.0% | |
| AGATGGTTTAGAAGAAGAGA+AGG | + | tig0023172:52273-52292 | Msa1405810:CDS | 35.0% | |
| TTAGAAGAAGAGAAGGAAGT+TGG | + | tig0023172:52280-52299 | Msa1405810:CDS | 35.0% | |
| AAGAAGAGAAGGAAGTTGGA+AGG | + | tig0023172:52284-52303 | Msa1405810:CDS | 40.0% | |
| AGAAGAGAAGGAAGTTGGAA+GGG | + | tig0023172:52285-52304 | Msa1405810:CDS | 40.0% | |
| ACCAATCCATACCTCTACCA+TGG | - | tig0023172:52194-52213 | None:intergenic | 45.0% | |
| AGTTGGAAGGGAAGCTCAAA+AGG | + | tig0023172:52297-52316 | Msa1405810:CDS | 45.0% | |
| ! | GCCATGGTAGAGGTATGGAT+TGG | + | tig0023172:52190-52209 | Msa1405810:CDS | 50.0% |
| TGGAAGGGAAGCTCAAAAGG+AGG | + | tig0023172:52300-52319 | Msa1405810:CDS | 50.0% | |
| TGTCGGCCATGGTAGAGGTA+TGG | + | tig0023172:52185-52204 | Msa1405810:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023172 | gene | 52184 | 52402 | 52184 | ID=Msa1405810;Name=Msa1405810 |
| tig0023172 | mRNA | 52184 | 52402 | 52184 | ID=Msa1405810-mRNA-1;Parent=Msa1405810;Name=Msa1405810-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|72 |
| tig0023172 | exon | 52184 | 52402 | 52184 | ID=Msa1405810-mRNA-1:exon:24263;Parent=Msa1405810-mRNA-1 |
| tig0023172 | CDS | 52184 | 52402 | 52184 | ID=Msa1405810-mRNA-1:cds;Parent=Msa1405810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
