AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0874570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0874570 MtrunA17_Chr6g0455241 96.552 87 3 0 19 105 56 142 1.67e-58 176
Msa0874570 MtrunA17_Chr6g0455271 93.750 96 6 0 1 96 2 97 1.49e-50 156
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGCTTGCCTTCTCAAAA+TGG 0.248342 6_1:-10375052 Msa0874570:CDS
TCATGCAGCAAGAATGCTTT+TGG 0.265450 6_1:-10375155 Msa0874570:CDS
ACAACATCATTGAAGATAAA+AGG 0.287321 6_1:-10375241 Msa0874570:CDS
TTGTCTGATCAACAATGATT+TGG 0.352096 6_1:-10375203 Msa0874570:CDS
GTTAAGTGTCCTCCAGTTCA+AGG 0.438505 6_1:-10375082 Msa0874570:CDS
CAAACACAAACTGGTAGAAC+TGG 0.449515 6_1:-10375124 Msa0874570:CDS
CAAGTACGATAGCCTTGAAC+TGG 0.453081 6_1:+10375070 None:intergenic
GATTTGAGTCAAACACAAAC+TGG 0.464256 6_1:-10375133 Msa0874570:CDS
ATGGAGGTGGACCCAACAAT+AGG 0.474022 6_1:-10375033 Msa0874570:CDS
TAAGTACCACACCTATTGTT+GGG 0.495822 6_1:+10375022 None:intergenic
GTAATTGGATCAAGAAGATA+AGG 0.513118 6_1:+10375275 None:intergenic
GTAAGTACCACACCTATTGT+TGG 0.525645 6_1:+10375021 None:intergenic
TTGCCTTCTCAAAATGGAGG+TGG 0.550109 6_1:-10375046 Msa0874570:CDS
GTACGATAGCCTTGAACTGG+AGG 0.617148 6_1:+10375073 None:intergenic
TGCTTGCCTTCTCAAAATGG+AGG 0.620754 6_1:-10375049 Msa0874570:CDS
ACCAAAGAAGAAAATGAACA+AGG 0.627471 6_1:-10375314 None:intergenic
CCAAAGAAGAAAATGAACAA+GGG 0.643796 6_1:-10375313 None:intergenic
GATAAAAGGTTGTACCTCAG+AGG 0.662136 6_1:-10375227 Msa0874570:CDS
GGTAGAACTGGTAACAACAA+TGG 0.669601 6_1:-10375112 Msa0874570:CDS
GGTGGACCCAACAATAGGTG+TGG 0.689105 6_1:-10375028 Msa0874570:CDS
GTTGATCAGACAATCCTCTG+AGG 0.737752 6_1:+10375213 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTGCTTAGATGTAAT+TGG + chr6_1:10375050-10375069 None:intergenic 25.0%
! ACAACATCATTGAAGATAAA+AGG - chr6_1:10375066-10375085 Msa0874570:CDS 25.0%
GTAATTGGATCAAGAAGATA+AGG + chr6_1:10375035-10375054 None:intergenic 30.0%
TTGTCTGATCAACAATGATT+TGG - chr6_1:10375104-10375123 Msa0874570:CDS 30.0%
GATTTGAGTCAAACACAAAC+TGG - chr6_1:10375174-10375193 Msa0874570:CDS 35.0%
TAAGTACCACACCTATTGTT+GGG + chr6_1:10375288-10375307 None:intergenic 35.0%
GATAAAAGGTTGTACCTCAG+AGG - chr6_1:10375080-10375099 Msa0874570:CDS 40.0%
! TCATGCAGCAAGAATGCTTT+TGG - chr6_1:10375152-10375171 Msa0874570:CDS 40.0%
CAAACACAAACTGGTAGAAC+TGG - chr6_1:10375183-10375202 Msa0874570:CDS 40.0%
GGTAGAACTGGTAACAACAA+TGG - chr6_1:10375195-10375214 Msa0874570:CDS 40.0%
ACTTGCTTGCCTTCTCAAAA+TGG - chr6_1:10375255-10375274 Msa0874570:CDS 40.0%
GTAAGTACCACACCTATTGT+TGG + chr6_1:10375289-10375308 None:intergenic 40.0%
GTTGATCAGACAATCCTCTG+AGG + chr6_1:10375097-10375116 None:intergenic 45.0%
GTTAAGTGTCCTCCAGTTCA+AGG - chr6_1:10375225-10375244 Msa0874570:CDS 45.0%
! CAAGTACGATAGCCTTGAAC+TGG + chr6_1:10375240-10375259 None:intergenic 45.0%
TGCTTGCCTTCTCAAAATGG+AGG - chr6_1:10375258-10375277 Msa0874570:CDS 45.0%
TTGCCTTCTCAAAATGGAGG+TGG - chr6_1:10375261-10375280 Msa0874570:CDS 45.0%
! GTACGATAGCCTTGAACTGG+AGG + chr6_1:10375237-10375256 None:intergenic 50.0%
!! GGTCCACCTCCATTTTGAGA+AGG + chr6_1:10375267-10375286 None:intergenic 50.0%
ATGGAGGTGGACCCAACAAT+AGG - chr6_1:10375274-10375293 Msa0874570:CDS 50.0%
GGTGGACCCAACAATAGGTG+TGG - chr6_1:10375279-10375298 Msa0874570:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 10375006 10375323 10375006 ID=Msa0874570;Name=Msa0874570
chr6_1 mRNA 10375006 10375323 10375006 ID=Msa0874570-mRNA-1;Parent=Msa0874570;Name=Msa0874570-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|105
chr6_1 exon 10375006 10375323 10375006 ID=Msa0874570-mRNA-1:exon:3204;Parent=Msa0874570-mRNA-1
chr6_1 CDS 10375006 10375323 10375006 ID=Msa0874570-mRNA-1:cds;Parent=Msa0874570-mRNA-1
Gene Sequence

>Msa0874570

ATGAACAAGGGTGTTTCACTATTGTTCCTTATCTTCTTGATCCAATTACATCTAAGCAAAACAACATCATTGAAGATAAAAGGTTGTACCTCAGAGGATTGTCTGATCAACAATGATTTGGAATCAGAAGAATTTTCTATGAGTTCTCATGCAGCAAGAATGCTTTTGGATTTGAGTCAAACACAAACTGGTAGAACTGGTAACAACAATGGTGCTGCTGTTAAGTGTCCTCCAGTTCAAGGCTATCGTACTTGCTTGCCTTCTCAAAATGGAGGTGGACCCAACAATAGGTGTGGTACTTACAATAGAGTTTGCTAA

Protein sequence

>Msa0874570

MNKGVSLLFLIFLIQLHLSKTTSLKIKGCTSEDCLINNDLESEEFSMSSHAARMLLDLSQTQTGRTGNNNGAAVKCPPVQGYRTCLPSQNGGGPNNRCGTYNRVC*