Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1412280 | sp|P31110|TLP_ORYSJ | 59.119 | 159 | 59 | 4 | 19 | 176 | 24 | 177 | 5.71e-59 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1412280 | A0A072TVX9 | 71.751 | 177 | 48 | 2 | 1 | 176 | 1 | 176 | 3.83e-88 | 265 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0009520 | Msa1412280 | 0.807241 | 5.565553e-50 | -8.615850e-47 |
| Msa0014790 | Msa1412280 | 0.800158 | 1.643479e-48 | -8.615850e-47 |
| Msa0032290 | Msa1412280 | 0.812138 | 4.929634e-51 | -8.615850e-47 |
| Msa0089010 | Msa1412280 | 0.806780 | 6.966227e-50 | -8.615850e-47 |
| Msa0104360 | Msa1412280 | 0.806723 | 7.162590e-50 | -8.615850e-47 |
| Msa0116510 | Msa1412280 | 0.802037 | 6.785601e-49 | -8.615850e-47 |
| Msa1351070 | Msa1412280 | 0.809332 | 1.993743e-50 | -8.615850e-47 |
| Msa1412280 | Msa1459170 | 0.826426 | 2.730131e-54 | -8.615850e-47 |
| Msa0607390 | Msa1412280 | 0.806389 | 8.423644e-50 | -8.615850e-47 |
| Msa0607410 | Msa1412280 | 0.804268 | 2.343371e-49 | -8.615850e-47 |
| Msa0627890 | Msa1412280 | 0.807605 | 4.659213e-50 | -8.615850e-47 |
| Msa0630000 | Msa1412280 | 0.808548 | 2.934629e-50 | -8.615850e-47 |
| Msa0630060 | Msa1412280 | 0.809970 | 1.453822e-50 | -8.615850e-47 |
| Msa0654020 | Msa1412280 | 0.814264 | 1.683643e-51 | -8.615850e-47 |
| Msa0733400 | Msa1412280 | 0.819812 | 9.539457e-53 | -8.615850e-47 |
| Msa0752580 | Msa1412280 | 0.832004 | 1.210366e-55 | -8.615850e-47 |
| Msa0754500 | Msa1412280 | 0.826614 | 2.463502e-54 | -8.615850e-47 |
| Msa0811940 | Msa1412280 | 0.806905 | 6.555187e-50 | -8.615850e-47 |
| Msa0830900 | Msa1412280 | 0.811561 | 6.582261e-51 | -8.615850e-47 |
| Msa0153440 | Msa1412280 | 0.802179 | 6.344209e-49 | -8.615850e-47 |
| Msa0180290 | Msa1412280 | 0.843523 | 1.329977e-58 | -8.615850e-47 |
| Msa0203070 | Msa1412280 | 0.808099 | 3.658226e-50 | -8.615850e-47 |
| Msa0211670 | Msa1412280 | 0.816726 | 4.766296e-52 | -8.615850e-47 |
| Msa0232860 | Msa1412280 | 0.873655 | 1.242753e-67 | -8.615850e-47 |
| Msa1272960 | Msa1412280 | 0.809739 | 1.630696e-50 | -8.615850e-47 |
| Msa1279310 | Msa1412280 | 0.804045 | 2.608242e-49 | -8.615850e-47 |
| Msa1279850 | Msa1412280 | 0.825726 | 4.006072e-54 | -8.615850e-47 |
| Msa1279860 | Msa1412280 | 0.804063 | 2.586056e-49 | -8.615850e-47 |
| Msa1285830 | Msa1412280 | 0.802596 | 5.204793e-49 | -8.615850e-47 |
| Msa1296650 | Msa1412280 | 0.802206 | 6.262860e-49 | -8.615850e-47 |
| Msa1340880 | Msa1412280 | 0.811431 | 7.023978e-51 | -8.615850e-47 |
| Msa0389050 | Msa1412280 | 0.837527 | 4.931668e-57 | -8.615850e-47 |
| Msa1006600 | Msa1412280 | 0.800389 | 1.474890e-48 | -8.615850e-47 |
| Msa1006680 | Msa1412280 | 0.828887 | 7.001546e-55 | -8.615850e-47 |
| Msa1009150 | Msa1412280 | 0.812045 | 5.166260e-51 | -8.615850e-47 |
| Msa1009170 | Msa1412280 | 0.834712 | 2.557539e-56 | -8.615850e-47 |
| Msa1009220 | Msa1412280 | 0.811442 | 6.987983e-51 | -8.615850e-47 |
| Msa1050500 | Msa1412280 | 0.816403 | 5.630455e-52 | -8.615850e-47 |
| Msa1098380 | Msa1412280 | 0.816513 | 5.321497e-52 | -8.615850e-47 |
| Msa0254190 | Msa1412280 | 0.819230 | 1.295643e-52 | -8.615850e-47 |
| Msa1107830 | Msa1412280 | 0.805494 | 1.299545e-49 | -8.615850e-47 |
| Msa1109590 | Msa1412280 | 0.800803 | 1.213996e-48 | -8.615850e-47 |
| Msa1135660 | Msa1412280 | 0.811075 | 8.394410e-51 | -8.615850e-47 |
| Msa1138450 | Msa1412280 | 0.829820 | 4.155216e-55 | -8.615850e-47 |
| Msa1138480 | Msa1412280 | 0.863281 | 2.784765e-64 | -8.615850e-47 |
| Msa1138490 | Msa1412280 | 0.843920 | 1.040984e-58 | -8.615850e-47 |
| Msa1140460 | Msa1412280 | 0.834836 | 2.380156e-56 | -8.615850e-47 |
| Msa1182780 | Msa1412280 | 0.841399 | 4.865127e-58 | -8.615850e-47 |
| Msa0558710 | Msa1412280 | 0.825497 | 4.539011e-54 | -8.615850e-47 |
| Msa0587670 | Msa1412280 | 0.829185 | 5.929041e-55 | -8.615850e-47 |
| Msa0589610 | Msa1412280 | 0.820185 | 7.840132e-53 | -8.615850e-47 |
| Msa0593250 | Msa1412280 | 0.814507 | 1.487468e-51 | -8.615850e-47 |
| Msa0593260 | Msa1412280 | 0.809996 | 1.435404e-50 | -8.615850e-47 |
| Msa0853500 | Msa1412280 | 0.816372 | 5.721966e-52 | -8.615850e-47 |
| Msa0867810 | Msa1412280 | 0.819046 | 1.426332e-52 | -8.615850e-47 |
| Msa0883350 | Msa1412280 | 0.806235 | 9.077744e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1412280 | MtrunA17_Chr8g0386351 | 71.751 | 177 | 48 | 2 | 1 | 176 | 43 | 218 | 1.07e-91 | 266 |
| Msa1412280 | MtrunA17_Chr8g0386341 | 42.152 | 223 | 79 | 5 | 2 | 176 | 28 | 248 | 6.25e-51 | 164 |
| Msa1412280 | MtrunA17_Chr5g0397821 | 42.512 | 207 | 68 | 5 | 19 | 176 | 21 | 225 | 1.97e-44 | 147 |
| Msa1412280 | MtrunA17_Chr5g0397831 | 45.122 | 164 | 81 | 5 | 1 | 157 | 1 | 162 | 2.02e-39 | 134 |
| Msa1412280 | MtrunA17_Chr1g0154591 | 33.036 | 224 | 83 | 7 | 19 | 176 | 10 | 232 | 4.78e-26 | 99.4 |
| Msa1412280 | MtrunA17_Chr2g0311531 | 35.000 | 200 | 83 | 8 | 21 | 176 | 26 | 222 | 4.15e-25 | 97.4 |
| Msa1412280 | MtrunA17_Chr3g0141341 | 39.370 | 127 | 62 | 3 | 19 | 134 | 9 | 131 | 1.32e-23 | 93.2 |
| Msa1412280 | MtrunA17_Chr4g0037331 | 38.571 | 140 | 74 | 5 | 1 | 129 | 1 | 139 | 1.72e-23 | 94.7 |
| Msa1412280 | MtrunA17_Chr5g0424221 | 42.105 | 133 | 66 | 5 | 1 | 129 | 11 | 136 | 2.45e-22 | 90.9 |
| Msa1412280 | MtrunA17_Chr6g0482741 | 35.000 | 140 | 80 | 5 | 1 | 132 | 1 | 137 | 3.19e-22 | 89.7 |
| Msa1412280 | MtrunA17_Chr5g0405611 | 30.736 | 231 | 96 | 10 | 6 | 176 | 82 | 308 | 8.87e-22 | 90.5 |
| Msa1412280 | MtrunA17_Chr8g0392961 | 42.609 | 115 | 55 | 5 | 26 | 132 | 31 | 142 | 1.67e-21 | 87.8 |
| Msa1412280 | MtrunA17_Chr8g0386361 | 75.439 | 57 | 10 | 2 | 1 | 57 | 1 | 53 | 4.66e-21 | 83.6 |
| Msa1412280 | MtrunA17_Chr8g0386361 | 73.913 | 46 | 12 | 0 | 131 | 176 | 73 | 118 | 1.09e-19 | 79.7 |
| Msa1412280 | MtrunA17_Chr5g0428801 | 39.080 | 174 | 84 | 10 | 3 | 157 | 4 | 174 | 4.86e-21 | 87.8 |
| Msa1412280 | MtrunA17_Chr8g0351851 | 35.542 | 166 | 93 | 7 | 1 | 155 | 1 | 163 | 5.20e-21 | 86.3 |
| Msa1412280 | MtrunA17_Chr8g0352471 | 37.143 | 140 | 74 | 6 | 1 | 131 | 9 | 143 | 4.07e-20 | 85.1 |
| Msa1412280 | MtrunA17_Chr4g0037351 | 39.024 | 164 | 82 | 10 | 3 | 150 | 5 | 166 | 6.47e-20 | 84.7 |
| Msa1412280 | MtrunA17_Chr8g0371771 | 33.750 | 160 | 88 | 7 | 17 | 163 | 26 | 180 | 8.14e-20 | 84.3 |
| Msa1412280 | MtrunA17_Chr2g0312181 | 35.404 | 161 | 88 | 7 | 1 | 151 | 1 | 155 | 1.50e-19 | 82.4 |
| Msa1412280 | MtrunA17_Chr7g0239131 | 34.078 | 179 | 101 | 9 | 1 | 164 | 1 | 177 | 1.63e-19 | 82.4 |
| Msa1412280 | MtrunA17_Chr3g0142781 | 29.707 | 239 | 97 | 9 | 8 | 176 | 12 | 249 | 3.87e-19 | 82.8 |
| Msa1412280 | MtrunA17_Chr8g0371781 | 42.276 | 123 | 61 | 6 | 16 | 129 | 22 | 143 | 6.52e-19 | 82.0 |
| Msa1412280 | MtrunA17_Chr1g0180231 | 31.217 | 189 | 103 | 9 | 1 | 175 | 1 | 176 | 1.74e-18 | 79.7 |
| Msa1412280 | MtrunA17_Chr1g0179871 | 31.217 | 189 | 103 | 9 | 1 | 175 | 1 | 176 | 1.74e-18 | 79.7 |
| Msa1412280 | MtrunA17_Chr8g0357311 | 36.232 | 138 | 74 | 6 | 1 | 129 | 9 | 141 | 2.52e-18 | 79.7 |
| Msa1412280 | MtrunA17_Chr2g0311561 | 29.694 | 229 | 86 | 10 | 19 | 176 | 8 | 232 | 4.44e-18 | 79.3 |
| Msa1412280 | MtrunA17_Chr1g0156711 | 39.416 | 137 | 71 | 7 | 3 | 129 | 4 | 138 | 6.01e-18 | 79.3 |
| Msa1412280 | MtrunA17_Chr1g0179891 | 30.978 | 184 | 100 | 9 | 6 | 175 | 1 | 171 | 6.87e-18 | 78.2 |
| Msa1412280 | MtrunA17_Chr5g0405581 | 35.294 | 136 | 77 | 6 | 3 | 129 | 8 | 141 | 1.08e-17 | 78.6 |
| Msa1412280 | MtrunA17_Chr1g0180121 | 27.869 | 244 | 106 | 10 | 1 | 176 | 1 | 242 | 2.95e-17 | 76.3 |
| Msa1412280 | MtrunA17_Chr3g0112091 | 32.432 | 148 | 71 | 7 | 11 | 137 | 2 | 141 | 2.15e-16 | 73.9 |
| Msa1412280 | MtrunA17_Chr2g0311521 | 29.515 | 227 | 87 | 11 | 21 | 176 | 23 | 247 | 2.70e-16 | 75.9 |
| Msa1412280 | MtrunA17_Chr6g0452331 | 37.209 | 129 | 60 | 7 | 16 | 129 | 23 | 145 | 2.87e-16 | 74.3 |
| Msa1412280 | MtrunA17_Chr4g0031221 | 35.156 | 128 | 73 | 4 | 11 | 129 | 18 | 144 | 3.66e-16 | 73.6 |
| Msa1412280 | MtrunA17_Chr1g0179851 | 33.121 | 157 | 89 | 8 | 3 | 150 | 1 | 150 | 5.33e-16 | 73.2 |
| Msa1412280 | MtrunA17_Chr5g0406771 | 36.296 | 135 | 74 | 6 | 12 | 135 | 3 | 136 | 1.54e-15 | 70.5 |
| Msa1412280 | MtrunA17_Chr1g0180161 | 30.818 | 159 | 95 | 8 | 1 | 150 | 1 | 153 | 1.67e-15 | 72.0 |
| Msa1412280 | MtrunA17_Chr1g0180221 | 30.818 | 159 | 95 | 7 | 1 | 150 | 41 | 193 | 2.74e-15 | 71.6 |
| Msa1412280 | MtrunA17_Chr7g0265011 | 28.455 | 246 | 94 | 12 | 8 | 176 | 47 | 287 | 3.28e-15 | 71.6 |
| Msa1412280 | MtrunA17_Chr2g0311551 | 28.889 | 225 | 89 | 11 | 21 | 176 | 22 | 244 | 2.99e-13 | 67.0 |
| Msa1412280 | MtrunA17_Chr2g0311501 | 39.130 | 92 | 52 | 4 | 62 | 150 | 5 | 95 | 2.61e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1412280 | AT4G11650.1 | 43.556 | 225 | 76 | 6 | 1 | 176 | 1 | 223 | 2.96e-51 | 164 |
| Msa1412280 | AT1G77700.3 | 47.826 | 115 | 54 | 3 | 18 | 129 | 57 | 168 | 1.19e-26 | 103 |
| Msa1412280 | AT1G77700.2 | 47.826 | 115 | 54 | 3 | 18 | 129 | 57 | 168 | 1.87e-26 | 102 |
| Msa1412280 | AT1G77700.1 | 47.826 | 115 | 54 | 3 | 18 | 129 | 85 | 196 | 2.88e-26 | 102 |
| Msa1412280 | AT1G75030.1 | 40.580 | 138 | 70 | 4 | 3 | 129 | 1 | 137 | 1.33e-23 | 93.6 |
| Msa1412280 | AT1G75050.1 | 36.970 | 165 | 93 | 5 | 1 | 155 | 4 | 167 | 1.66e-23 | 93.6 |
| Msa1412280 | AT5G24620.1 | 34.091 | 176 | 98 | 7 | 3 | 162 | 6 | 179 | 1.27e-22 | 93.6 |
| Msa1412280 | AT5G24620.4 | 34.091 | 176 | 98 | 7 | 3 | 162 | 61 | 234 | 1.40e-22 | 94.0 |
| Msa1412280 | AT4G38660.1 | 43.077 | 130 | 60 | 7 | 12 | 129 | 17 | 144 | 1.46e-22 | 92.8 |
| Msa1412280 | AT5G24620.3 | 34.091 | 176 | 98 | 7 | 3 | 162 | 82 | 255 | 1.74e-22 | 93.6 |
| Msa1412280 | AT4G38660.2 | 43.902 | 123 | 58 | 6 | 16 | 129 | 2 | 122 | 3.41e-22 | 91.3 |
| Msa1412280 | AT1G75040.1 | 31.760 | 233 | 92 | 9 | 8 | 176 | 10 | 239 | 1.75e-21 | 87.8 |
| Msa1412280 | AT1G19320.1 | 34.254 | 181 | 93 | 8 | 3 | 164 | 9 | 182 | 4.16e-21 | 87.0 |
| Msa1412280 | AT5G40020.1 | 32.065 | 184 | 93 | 10 | 1 | 159 | 1 | 177 | 2.50e-20 | 85.1 |
| Msa1412280 | AT2G17860.1 | 40.000 | 140 | 72 | 7 | 1 | 129 | 1 | 139 | 7.70e-20 | 84.0 |
| Msa1412280 | AT1G75800.1 | 37.410 | 139 | 76 | 6 | 1 | 129 | 1 | 138 | 7.83e-20 | 85.1 |
| Msa1412280 | AT4G38670.3 | 38.849 | 139 | 74 | 6 | 1 | 129 | 2 | 139 | 1.20e-19 | 84.0 |
| Msa1412280 | AT4G38670.1 | 38.849 | 139 | 74 | 6 | 1 | 129 | 2 | 139 | 1.20e-19 | 84.0 |
| Msa1412280 | AT1G73620.1 | 40.336 | 119 | 60 | 5 | 19 | 129 | 39 | 154 | 1.66e-19 | 83.2 |
| Msa1412280 | AT2G28790.2 | 36.145 | 166 | 83 | 9 | 1 | 146 | 24 | 186 | 1.90e-19 | 83.2 |
| Msa1412280 | AT2G28790.1 | 35.758 | 165 | 83 | 9 | 1 | 146 | 1 | 161 | 2.03e-19 | 82.8 |
| Msa1412280 | AT4G24180.6 | 36.620 | 142 | 74 | 6 | 3 | 129 | 14 | 154 | 6.88e-19 | 81.6 |
| Msa1412280 | AT4G36010.1 | 45.690 | 116 | 52 | 6 | 24 | 129 | 25 | 139 | 1.23e-18 | 81.3 |
| Msa1412280 | AT4G36010.2 | 45.690 | 116 | 52 | 6 | 24 | 129 | 25 | 139 | 1.23e-18 | 81.3 |
| Msa1412280 | AT4G24180.3 | 36.620 | 142 | 74 | 6 | 3 | 129 | 14 | 154 | 1.24e-18 | 81.6 |
| Msa1412280 | AT5G02140.2 | 32.370 | 173 | 100 | 7 | 7 | 164 | 6 | 176 | 1.33e-18 | 80.5 |
| Msa1412280 | AT4G24180.1 | 36.620 | 142 | 74 | 6 | 3 | 129 | 14 | 154 | 1.62e-18 | 81.3 |
| Msa1412280 | AT5G02140.1 | 32.370 | 173 | 100 | 7 | 7 | 164 | 6 | 176 | 1.65e-18 | 80.9 |
| Msa1412280 | AT1G18250.1 | 40.336 | 119 | 60 | 5 | 19 | 129 | 18 | 133 | 1.75e-18 | 80.1 |
| Msa1412280 | AT4G38670.2 | 41.129 | 124 | 62 | 6 | 16 | 129 | 1 | 123 | 2.28e-18 | 80.1 |
| Msa1412280 | AT1G18250.2 | 39.167 | 120 | 62 | 5 | 18 | 129 | 18 | 134 | 4.76e-18 | 79.0 |
| Msa1412280 | AT5G38280.2 | 34.395 | 157 | 87 | 8 | 6 | 149 | 5 | 158 | 1.03e-17 | 80.1 |
| Msa1412280 | AT5G38280.1 | 34.395 | 157 | 87 | 8 | 6 | 149 | 5 | 158 | 1.08e-17 | 80.1 |
| Msa1412280 | AT4G24180.5 | 35.664 | 143 | 76 | 6 | 2 | 129 | 8 | 149 | 1.21e-16 | 75.5 |
| Msa1412280 | AT1G20030.1 | 38.261 | 115 | 61 | 5 | 24 | 129 | 5 | 118 | 1.58e-16 | 75.5 |
| Msa1412280 | AT1G20030.2 | 38.261 | 115 | 61 | 5 | 24 | 129 | 22 | 135 | 1.67e-16 | 75.9 |
| Msa1412280 | AT4G24180.4 | 35.664 | 143 | 76 | 6 | 2 | 129 | 8 | 149 | 2.07e-16 | 75.5 |
| Msa1412280 | AT4G24180.2 | 35.664 | 143 | 76 | 6 | 2 | 129 | 8 | 149 | 2.30e-16 | 75.5 |
| Msa1412280 | AT1G70250.1 | 29.851 | 134 | 86 | 4 | 24 | 150 | 147 | 279 | 2.65e-14 | 70.5 |
Find 59 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACCTCATCTGCCCGAATT+TGG | 0.212186 | tig0023878:+29197 | Msa1412280:CDS |
| AACCTCATCTGCCCGAATTT+GGG | 0.245360 | tig0023878:+29198 | Msa1412280:CDS |
| AATTGGTTCGGCCCCAAATT+CGG | 0.284646 | tig0023878:-29210 | None:intergenic |
| CACTGCCTGAACCGTCAAAA+TGG | 0.324690 | tig0023878:-29234 | None:intergenic |
| ATTGGTTCGGCCCCAAATTC+GGG | 0.337201 | tig0023878:-29209 | None:intergenic |
| GTCAGAACCAAGTGTGAATT+CGG | 0.365389 | tig0023878:-29329 | None:intergenic |
| GGCAGTGATGGTTGTCAAAC+CGG | 0.387950 | tig0023878:+29250 | Msa1412280:CDS |
| ATTAATTTATCTGTGATTGA+TGG | 0.391582 | tig0023878:+29364 | Msa1412280:CDS |
| GCTGCTACTCCCGTCAATAA+AGG | 0.413360 | tig0023878:+29124 | Msa1412280:CDS |
| GTGAATTCGGCAAGGGTTAG+CGG | 0.425952 | tig0023878:-29316 | None:intergenic |
| AGGTTCCAGCTGGGATGTCC+AGG | 0.431939 | tig0023878:-29180 | None:intergenic |
| TGAATTCGGCAAGGGTTAGC+GGG | 0.433684 | tig0023878:-29315 | None:intergenic |
| CCGGGGAGATGATAAGCATC+AGG | 0.438940 | tig0023878:-29463 | None:intergenic |
| ACCAATGTTCCTTCACTGTC+TGG | 0.440354 | tig0023878:+29098 | Msa1412280:CDS |
| GCCCTTGATTGCAGCTCATC+CGG | 0.442995 | tig0023878:+29286 | Msa1412280:CDS |
| CGTCAAAATGGCAATTGGTT+CGG | 0.451344 | tig0023878:-29222 | None:intergenic |
| AACTGCTTACCTTTATTGAC+GGG | 0.462868 | tig0023878:-29133 | None:intergenic |
| AAGGTAAGCAGTTGGACAGC+AGG | 0.464476 | tig0023878:+29143 | Msa1412280:CDS |
| TACCGGATGAGCTGCAATCA+AGG | 0.472730 | tig0023878:-29288 | None:intergenic |
| GGATGTCCAGGGACCATGGT+TGG | 0.476649 | tig0023878:-29168 | None:intergenic |
| TGAACCGTCAAAATGGCAAT+TGG | 0.484702 | tig0023878:-29227 | None:intergenic |
| GAGGTTGAACTAAATTGCAT+TGG | 0.486855 | tig0023878:-29397 | None:intergenic |
| CGGGCAGATGAGGTTCCAGC+TGG | 0.497937 | tig0023878:-29190 | None:intergenic |
| AGGGCAATTAGAGTCCTTGC+AGG | 0.499355 | tig0023878:-29443 | None:intergenic |
| CAGGGGCCAACCATGGTCCC+TGG | 0.501402 | tig0023878:+29162 | Msa1412280:CDS |
| TGTATTCGTTCGTTTATCAC+CGG | 0.504853 | tig0023878:-29482 | None:intergenic |
| TCTGTGCTTGATATCTGCTC+AGG | 0.511370 | tig0023878:+29054 | Msa1412280:CDS |
| CAATTTAGTTCAACCTCTAG+TGG | 0.511442 | tig0023878:+29403 | Msa1412280:CDS |
| ACCCTTGCCGAATTCACACT+TGG | 0.518284 | tig0023878:+29322 | Msa1412280:CDS |
| CAACTGCTTACCTTTATTGA+CGG | 0.524802 | tig0023878:-29134 | None:intergenic |
| TGGTCCCTGGACATCCCAGC+TGG | 0.526345 | tig0023878:+29175 | Msa1412280:CDS |
| TTTGACGGTTCAGGCAGTGA+TGG | 0.534748 | tig0023878:+29238 | Msa1412280:CDS |
| ACCTCATCTGCCCGAATTTG+GGG | 0.535426 | tig0023878:+29199 | Msa1412280:CDS |
| AGGTAAGCAGTTGGACAGCA+GGG | 0.544502 | tig0023878:+29144 | Msa1412280:CDS |
| TTATTGACGGGAGTAGCAGC+TGG | 0.547273 | tig0023878:-29121 | None:intergenic |
| TACTTTGTTGCATCCACTAG+AGG | 0.548592 | tig0023878:-29416 | None:intergenic |
| GCTGGGATGTCCAGGGACCA+TGG | 0.550741 | tig0023878:-29172 | None:intergenic |
| GCCCCAAATTCGGGCAGATG+AGG | 0.552041 | tig0023878:-29200 | None:intergenic |
| GAATTCGGCAAGGGTTAGCG+GGG | 0.553998 | tig0023878:-29314 | None:intergenic |
| TGTCAAACCGGTGATTGTGC+TGG | 0.558483 | tig0023878:+29262 | Msa1412280:CDS |
| GCCAGACAGTGAAGGAACAT+TGG | 0.558598 | tig0023878:-29099 | None:intergenic |
| CCTGATGCTTATCATCTCCC+CGG | 0.578309 | tig0023878:+29463 | Msa1412280:CDS |
| AAGGGCACCAGCACAATCAC+CGG | 0.586421 | tig0023878:-29269 | None:intergenic |
| GTATTCGTTCGTTTATCACC+GGG | 0.586920 | tig0023878:-29481 | None:intergenic |
| CGTCAATAAAGGTAAGCAGT+TGG | 0.591090 | tig0023878:+29135 | Msa1412280:CDS |
| TGATATCTGCTCAGGCAGCA+AGG | 0.592249 | tig0023878:+29062 | Msa1412280:CDS |
| AGCAGCTGGCCAGACAGTGA+AGG | 0.595270 | tig0023878:-29107 | None:intergenic |
| CGAACGAATACATGCCCAGT+TGG | 0.595353 | tig0023878:+29493 | Msa1412280:CDS |
| AACCAAGTGTGAATTCGGCA+AGG | 0.607224 | tig0023878:-29324 | None:intergenic |
| ACCGGATGAGCTGCAATCAA+GGG | 0.609796 | tig0023878:-29287 | None:intergenic |
| TGGACAGCAGGGGCCAACCA+TGG | 0.613563 | tig0023878:+29155 | Msa1412280:CDS |
| ACCAAGTGTGAATTCGGCAA+GGG | 0.626520 | tig0023878:-29323 | None:intergenic |
| CAACAAAGTACTAACCTGCA+AGG | 0.631029 | tig0023878:+29429 | Msa1412280:CDS |
| GGTTCCAGCTGGGATGTCCA+GGG | 0.635953 | tig0023878:-29179 | None:intergenic |
| GGGCAGATGAGGTTCCAGCT+GGG | 0.641351 | tig0023878:-29189 | None:intergenic |
| CGGGGAGATGATAAGCATCA+GGG | 0.646159 | tig0023878:-29462 | None:intergenic |
| GGTAAGCAGTTGGACAGCAG+GGG | 0.653652 | tig0023878:+29145 | Msa1412280:CDS |
| AATTCGGCAAGGGTTAGCGG+GGG | 0.672413 | tig0023878:-29313 | None:intergenic |
| TATTCGTTCGTTTATCACCG+GGG | 0.759174 | tig0023878:-29480 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATTTATCTGTGATTGA+TGG | + | tig0023878:29364-29383 | Msa1412280:CDS | 20.0% |
| !! | TTTTATAGTTGGTACCAACT+GGG | - | tig0023878:29510-29529 | None:intergenic | 30.0% |
| !! | ATTTTATAGTTGGTACCAAC+TGG | - | tig0023878:29511-29530 | None:intergenic | 30.0% |
| ! | GCAAAAGACGATTTTATAGT+TGG | - | tig0023878:29521-29540 | None:intergenic | 30.0% |
| AACTGCTTACCTTTATTGAC+GGG | - | tig0023878:29136-29155 | None:intergenic | 35.0% | |
| CAACTGCTTACCTTTATTGA+CGG | - | tig0023878:29137-29156 | None:intergenic | 35.0% | |
| GAGGTTGAACTAAATTGCAT+TGG | - | tig0023878:29400-29419 | None:intergenic | 35.0% | |
| ! | CAATTTAGTTCAACCTCTAG+TGG | + | tig0023878:29403-29422 | Msa1412280:CDS | 35.0% |
| TGTATTCGTTCGTTTATCAC+CGG | - | tig0023878:29485-29504 | None:intergenic | 35.0% | |
| CGTCAATAAAGGTAAGCAGT+TGG | + | tig0023878:29135-29154 | Msa1412280:CDS | 40.0% | |
| CGTCAAAATGGCAATTGGTT+CGG | - | tig0023878:29225-29244 | None:intergenic | 40.0% | |
| !!! | CGAACCAATTGCCATTTTGA+CGG | + | tig0023878:29223-29242 | Msa1412280:CDS | 40.0% |
| TGAACCGTCAAAATGGCAAT+TGG | - | tig0023878:29230-29249 | None:intergenic | 40.0% | |
| !!! | AATTGCCATTTTGACGGTTC+AGG | + | tig0023878:29229-29248 | Msa1412280:CDS | 40.0% |
| GTCAGAACCAAGTGTGAATT+CGG | - | tig0023878:29332-29351 | None:intergenic | 40.0% | |
| TACTTTGTTGCATCCACTAG+AGG | - | tig0023878:29419-29438 | None:intergenic | 40.0% | |
| ! | CAACAAAGTACTAACCTGCA+AGG | + | tig0023878:29429-29448 | Msa1412280:CDS | 40.0% |
| TATTCGTTCGTTTATCACCG+GGG | - | tig0023878:29483-29502 | None:intergenic | 40.0% | |
| GTATTCGTTCGTTTATCACC+GGG | - | tig0023878:29484-29503 | None:intergenic | 40.0% | |
| TCTGTGCTTGATATCTGCTC+AGG | + | tig0023878:29054-29073 | Msa1412280:CDS | 45.0% | |
| ACCAATGTTCCTTCACTGTC+TGG | + | tig0023878:29098-29117 | Msa1412280:CDS | 45.0% | |
| AACCTCATCTGCCCGAATTT+GGG | + | tig0023878:29198-29217 | Msa1412280:CDS | 45.0% | |
| !! | AATTGGTTCGGCCCCAAATT+CGG | - | tig0023878:29213-29232 | None:intergenic | 45.0% |
| ACCAAGTGTGAATTCGGCAA+GGG | - | tig0023878:29326-29345 | None:intergenic | 45.0% | |
| AACCAAGTGTGAATTCGGCA+AGG | - | tig0023878:29327-29346 | None:intergenic | 45.0% | |
| TGATATCTGCTCAGGCAGCA+AGG | + | tig0023878:29062-29081 | Msa1412280:CDS | 50.0% | |
| GCCAGACAGTGAAGGAACAT+TGG | - | tig0023878:29102-29121 | None:intergenic | 50.0% | |
| TTATTGACGGGAGTAGCAGC+TGG | - | tig0023878:29124-29143 | None:intergenic | 50.0% | |
| GCTGCTACTCCCGTCAATAA+AGG | + | tig0023878:29124-29143 | Msa1412280:CDS | 50.0% | |
| AAGGTAAGCAGTTGGACAGC+AGG | + | tig0023878:29143-29162 | Msa1412280:CDS | 50.0% | |
| AGGTAAGCAGTTGGACAGCA+GGG | + | tig0023878:29144-29163 | Msa1412280:CDS | 50.0% | |
| GAACCTCATCTGCCCGAATT+TGG | + | tig0023878:29197-29216 | Msa1412280:CDS | 50.0% | |
| ACCTCATCTGCCCGAATTTG+GGG | + | tig0023878:29199-29218 | Msa1412280:CDS | 50.0% | |
| !! | ATTGGTTCGGCCCCAAATTC+GGG | - | tig0023878:29212-29231 | None:intergenic | 50.0% |
| CACTGCCTGAACCGTCAAAA+TGG | - | tig0023878:29237-29256 | None:intergenic | 50.0% | |
| TTTGACGGTTCAGGCAGTGA+TGG | + | tig0023878:29238-29257 | Msa1412280:CDS | 50.0% | |
| GGCAGTGATGGTTGTCAAAC+CGG | + | tig0023878:29250-29269 | Msa1412280:CDS | 50.0% | |
| TGTCAAACCGGTGATTGTGC+TGG | + | tig0023878:29262-29281 | Msa1412280:CDS | 50.0% | |
| ACCGGATGAGCTGCAATCAA+GGG | - | tig0023878:29290-29309 | None:intergenic | 50.0% | |
| TACCGGATGAGCTGCAATCA+AGG | - | tig0023878:29291-29310 | None:intergenic | 50.0% | |
| ! | TGAATTCGGCAAGGGTTAGC+GGG | - | tig0023878:29318-29337 | None:intergenic | 50.0% |
| GTGAATTCGGCAAGGGTTAG+CGG | - | tig0023878:29319-29338 | None:intergenic | 50.0% | |
| ACCCTTGCCGAATTCACACT+TGG | + | tig0023878:29322-29341 | Msa1412280:CDS | 50.0% | |
| AGGGCAATTAGAGTCCTTGC+AGG | - | tig0023878:29446-29465 | None:intergenic | 50.0% | |
| CGGGGAGATGATAAGCATCA+GGG | - | tig0023878:29465-29484 | None:intergenic | 50.0% | |
| CCTGATGCTTATCATCTCCC+CGG | + | tig0023878:29463-29482 | Msa1412280:CDS | 50.0% | |
| CGAACGAATACATGCCCAGT+TGG | + | tig0023878:29493-29512 | Msa1412280:CDS | 50.0% | |
| GGTAAGCAGTTGGACAGCAG+GGG | + | tig0023878:29145-29164 | Msa1412280:CDS | 55.0% | |
| !! | AAGGGCACCAGCACAATCAC+CGG | - | tig0023878:29272-29291 | None:intergenic | 55.0% |
| GCCCTTGATTGCAGCTCATC+CGG | + | tig0023878:29286-29305 | Msa1412280:CDS | 55.0% | |
| ! | AAGGGTTAGCGGGGGTTTAC+CGG | - | tig0023878:29308-29327 | None:intergenic | 55.0% |
| ! | AATTCGGCAAGGGTTAGCGG+GGG | - | tig0023878:29316-29335 | None:intergenic | 55.0% |
| ! | GAATTCGGCAAGGGTTAGCG+GGG | - | tig0023878:29317-29336 | None:intergenic | 55.0% |
| CCGGGGAGATGATAAGCATC+AGG | - | tig0023878:29466-29485 | None:intergenic | 55.0% | |
| AGCAGCTGGCCAGACAGTGA+AGG | - | tig0023878:29110-29129 | None:intergenic | 60.0% | |
| GGATGTCCAGGGACCATGGT+TGG | - | tig0023878:29171-29190 | None:intergenic | 60.0% | |
| GGTTCCAGCTGGGATGTCCA+GGG | - | tig0023878:29182-29201 | None:intergenic | 60.0% | |
| AGGTTCCAGCTGGGATGTCC+AGG | - | tig0023878:29183-29202 | None:intergenic | 60.0% | |
| GGGCAGATGAGGTTCCAGCT+GGG | - | tig0023878:29192-29211 | None:intergenic | 60.0% | |
| GCCCCAAATTCGGGCAGATG+AGG | - | tig0023878:29203-29222 | None:intergenic | 60.0% | |
| TGGACAGCAGGGGCCAACCA+TGG | + | tig0023878:29155-29174 | Msa1412280:CDS | 65.0% | |
| GCTGGGATGTCCAGGGACCA+TGG | - | tig0023878:29175-29194 | None:intergenic | 65.0% | |
| TGGTCCCTGGACATCCCAGC+TGG | + | tig0023878:29175-29194 | Msa1412280:CDS | 65.0% | |
| CGGGCAGATGAGGTTCCAGC+TGG | - | tig0023878:29193-29212 | None:intergenic | 65.0% | |
| CAGGGGCCAACCATGGTCCC+TGG | + | tig0023878:29162-29181 | Msa1412280:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023878 | gene | 29016 | 29546 | 29016 | ID=Msa1412280;Name=Msa1412280 |
| tig0023878 | mRNA | 29016 | 29546 | 29016 | ID=Msa1412280-mRNA-1;Parent=Msa1412280;Name=Msa1412280-mRNA-1;_AED=0.37;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|176 |
| tig0023878 | exon | 29016 | 29546 | 29016 | ID=Msa1412280-mRNA-1:exon:20519;Parent=Msa1412280-mRNA-1 |
| tig0023878 | CDS | 29016 | 29546 | 29016 | ID=Msa1412280-mRNA-1:cds;Parent=Msa1412280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
