AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1417640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417640 MtrunA17_Chr3g0144601 80.963 457 63 9 1 453 1 437 0.0 672
Msa1417640 MtrunA17_Chr3g0145531 80.963 457 63 9 1 453 1 437 0.0 665
Msa1417640 MtrunA17_Chr3g0082361 78.333 120 25 1 195 314 56 174 1.44e-57 187
Msa1417640 MtrunA17_Chr3g0107471 32.443 262 120 5 217 449 387 620 1.77e-30 124
Msa1417640 MtrunA17_Chr3g0107471 33.645 214 112 9 6 215 8 195 1.44e-17 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417640 AT1G15720.1 28.689 366 193 16 90 447 81 386 1.43e-12 69.3

Find 128 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCATCATTGTTAACATTT+AGG 0.186976 tig0024501:+65824 None:intergenic
CTGCTGGATCGATTGGAATT+TGG 0.216339 tig0024501:+67289 None:intergenic
CTGCTTGGAGGTAGTGTTTA+GGG 0.228026 tig0024501:+66872 None:intergenic
CTCTTGAATGGCTTTCCTTC+TGG 0.254243 tig0024501:+66705 None:intergenic
TCCAAGCAGTTGACAGAATA+TGG 0.254939 tig0024501:-66858 Msa1417640:CDS
TAAGTGAATCCCCGTTCTTT+TGG 0.273307 tig0024501:+66010 None:intergenic
TAACTCCAAAATTGAGATTC+TGG 0.273343 tig0024501:-66795 Msa1417640:CDS
ATGGCATCGCTAATAGGATT+TGG 0.282972 tig0024501:+66973 None:intergenic
GTCTGGCATACAAATAGAAT+TGG 0.288931 tig0024501:+65942 None:intergenic
TGAGTACGAGCAGAACTATT+TGG 0.294065 tig0024501:+65742 None:intergenic
AAAGACAATGGAAGGCTTAA+AGG 0.299896 tig0024501:-66120 Msa1417640:CDS
AAGAGAAAGGAATTTGAAAA+TGG 0.305804 tig0024501:-67250 Msa1417640:exon
ACTGCTTGGAGGTAGTGTTT+AGG 0.310482 tig0024501:+66871 None:intergenic
TATAGAGAGTGAGCATATTA+AGG 0.313329 tig0024501:-67178 Msa1417640:CDS
CCTTTAAAACAAAAGTTACT+TGG 0.313965 tig0024501:+64325 None:intergenic
TGATTGTGAAGTAAAATTCT+AGG 0.316653 tig0024501:+64395 None:intergenic
TCGCTAATAGGATTTGGGTT+AGG 0.335413 tig0024501:+66979 None:intergenic
GATTTCATGGCATCGCTAAT+AGG 0.336194 tig0024501:+66967 None:intergenic
CGCTAATAGGATTTGGGTTA+GGG 0.343442 tig0024501:+66980 None:intergenic
GCTCCTTCTGCTGCTCATAA+TGG 0.353339 tig0024501:-66616 Msa1417640:intron
TCAGAATTGGAGAAGGAAAC+TGG 0.354877 tig0024501:-64746 Msa1417640:intron
CTCTTCTCTTCTAACGCTGC+TGG 0.355705 tig0024501:+67273 None:intergenic
TGGCATCGCTAATAGGATTT+GGG 0.372317 tig0024501:+66974 None:intergenic
CATCTTCCGATTATCGATTT+CGG 0.378562 tig0024501:+67128 None:intergenic
GATGATGAACATCACTTTGA+TGG 0.379549 tig0024501:+65779 None:intergenic
CCATTCATCGATAGATGCTT+TGG 0.387362 tig0024501:+67065 None:intergenic
GTAGACATAAGCAGATTTCA+TGG 0.396640 tig0024501:+66954 None:intergenic
GGAAAGGTTAGGGTGGTGTT+TGG 0.397081 tig0024501:-67229 Msa1417640:CDS
TGTTGTTGTTGTTGAGGTAA+TGG 0.400125 tig0024501:-66082 Msa1417640:CDS
TTTGTATGCCAGACATTGTA+AGG 0.401380 tig0024501:-65933 Msa1417640:CDS
GAAGAAGCTTTCTCCGTTGA+AGG 0.405658 tig0024501:-65201 Msa1417640:CDS
CACCTGCACCTGCTGCTTCA+AGG 0.406135 tig0024501:+66905 None:intergenic
ATATATACAGCCCTTCTGTT+TGG 0.407004 tig0024501:+64504 None:intergenic
GGAAGTTGCCTTCGTAAGAT+TGG 0.411891 tig0024501:+65801 None:intergenic
TGGTGTTTGGAATTCGTTCT+TGG 0.411975 tig0024501:-67216 Msa1417640:CDS
TTAACGAGGCCCAAAAGAAC+GGG 0.420375 tig0024501:-66020 Msa1417640:CDS
TCCATATTCTGTCAACTGCT+TGG 0.421745 tig0024501:+66857 None:intergenic
TTTAGGCAAGCATGTGGGTT+GGG 0.424815 tig0024501:+65841 None:intergenic
AAAACTACTTCTAATTGATT+GGG 0.433610 tig0024501:-67016 Msa1417640:CDS
GCATGTATCGTGAGTTGAAA+AGG 0.433870 tig0024501:+66752 None:intergenic
GTGTCTGATATTGCGTTAGC+AGG 0.434957 tig0024501:-64355 Msa1417640:three_prime_UTR
TGAATTTGGAAGGTATGTTC+AGG 0.437724 tig0024501:-64435 Msa1417640:three_prime_UTR
GCAAATAACTTCTTCCCTCC+AGG 0.443932 tig0024501:+66818 None:intergenic
TTTGGGTTAGGGTTTGAAGA+AGG 0.445106 tig0024501:+66991 None:intergenic
TGGACAACAACTCATCTTCA+AGG 0.456297 tig0024501:+67085 None:intergenic
AATGGGAAGAGGATAATCTA+AGG 0.459156 tig0024501:-65127 Msa1417640:CDS
GGAGAAAGCTTCTTCCTCTC+TGG 0.461592 tig0024501:+65209 None:intergenic
CACAAATCAGGAGCCACAAA+TGG 0.462731 tig0024501:-65890 Msa1417640:CDS
TACTACTGTTGTTGTTGTTG+AGG 0.467147 tig0024501:-66088 Msa1417640:CDS
TGGAGAAATTTGAAGCGGTC+TGG 0.467731 tig0024501:-64588 Msa1417640:CDS
ATTTGAAAATGGAAAGGTTA+GGG 0.470688 tig0024501:-67239 Msa1417640:exon
TCTAACGCTGCTGGATCGAT+TGG 0.470932 tig0024501:+67282 None:intergenic
ACATCAGACCTTACAATGTC+TGG 0.471043 tig0024501:+65925 None:intergenic
GTACATTTGCATCTTTACTC+TGG 0.471288 tig0024501:+65863 None:intergenic
TGATTCGATCGATGGATTGC+AGG 0.471350 tig0024501:-65273 Msa1417640:CDS
AATTTGAAAATGGAAAGGTT+AGG 0.473595 tig0024501:-67240 Msa1417640:exon
GAAGATGAAAATGCTTCTGT+TGG 0.479184 tig0024501:-67112 Msa1417640:CDS
TACCTTGAAGCAGCAGGTGC+AGG 0.480680 tig0024501:-66907 Msa1417640:CDS
GCAACAGAGTGAAGAAAGGA+AGG 0.481758 tig0024501:+66640 None:intergenic
GGCAATTGAAGACACAAATC+AGG 0.483604 tig0024501:-65902 Msa1417640:CDS
CAGTCACCGAAATCGATAAT+CGG 0.485524 tig0024501:-67134 Msa1417640:CDS
GATGCTCGGAATAACCTTGT+AGG 0.496320 tig0024501:-66664 Msa1417640:CDS
TCTGGCATACAAATAGAATT+GGG 0.497185 tig0024501:+65943 None:intergenic
ATATTCTGTCAACTGCTTGG+AGG 0.502799 tig0024501:+66860 None:intergenic
ATAAGTTAGATACATCTTGA+GGG 0.506213 tig0024501:+64284 None:intergenic
CTATTTGGTTTCATCAAGCT+AGG 0.506498 tig0024501:+65757 None:intergenic
AAGTACATACAGTTTAATTC+CGG 0.506549 tig0024501:+65103 None:intergenic
TTGGGTTAGGGTTTGAAGAA+GGG 0.508995 tig0024501:+66992 None:intergenic
ATGAAAATGCTTCTGTTGGA+AGG 0.511456 tig0024501:-67108 Msa1417640:CDS
TAGAAGAGAAGAGAAGAGAA+AGG 0.511915 tig0024501:-67263 Msa1417640:five_prime_UTR
CCAAAGCATCTATCGATGAA+TGG 0.516478 tig0024501:-67065 Msa1417640:CDS
TGAAAAGGGAAGCATCGATT+CGG 0.518415 tig0024501:+66767 None:intergenic
TTGTCTTTGTCGAGTTCCAT+CGG 0.525502 tig0024501:+66135 None:intergenic
AGCAGCAACAGAGTGAAGAA+AGG 0.532298 tig0024501:+66636 None:intergenic
AACGCAATATCAGACACTAC+TGG 0.532623 tig0024501:+64361 None:intergenic
ATCAGTTCATGCCAAACAGA+AGG 0.535655 tig0024501:-64515 Msa1417640:three_prime_UTR
CATGTATCGTGAGTTGAAAA+GGG 0.536326 tig0024501:+66753 None:intergenic
TAGTGGGATGATTCGATCGA+TGG 0.537188 tig0024501:-65281 Msa1417640:CDS
GATTGCAGGCAGGAACATCA+AGG 0.543132 tig0024501:-65259 Msa1417640:CDS
AATGGCTCGCTGCTTTATCA+AGG 0.544070 tig0024501:+64238 None:intergenic
GATTGTGAAGTAAAATTCTA+GGG 0.544638 tig0024501:+64396 None:intergenic
TTCACTTAAATGCAATTCTG+TGG 0.545122 tig0024501:-65995 Msa1417640:CDS
TCGATCGATGGATTGCAGGC+AGG 0.547141 tig0024501:-65269 Msa1417640:CDS
TTTGGTTCATACTCCTTCAA+CGG 0.551515 tig0024501:+65188 None:intergenic
AATAAGTTAGATACATCTTG+AGG 0.553492 tig0024501:+64283 None:intergenic
AAAATGGAGTCAATGGGAAG+AGG 0.556320 tig0024501:-65138 Msa1417640:CDS
ATTTAGGCAAGCATGTGGGT+TGG 0.556821 tig0024501:+65840 None:intergenic
AAAGGAATTTGAAAATGGAA+AGG 0.559315 tig0024501:-67245 Msa1417640:exon
GAACCGTAGGTTTCAAAACC+TGG 0.560313 tig0024501:-66836 Msa1417640:CDS
ATGATGAACATCACTTTGAT+GGG 0.562151 tig0024501:+65780 None:intergenic
GAAGAGGATAATCTAAGGAC+CGG 0.564580 tig0024501:-65122 Msa1417640:CDS
GTTAACGAGGCCCAAAAGAA+CGG 0.567267 tig0024501:-66021 Msa1417640:CDS
GAAGAAAGGAAGGGCCTACA+AGG 0.570430 tig0024501:+66650 None:intergenic
CTTCATTGTCTGATACCAGA+AGG 0.585689 tig0024501:-66720 Msa1417640:CDS
AACTCGACAAAGACAATGGA+AGG 0.587275 tig0024501:-66128 Msa1417640:CDS
CAATGGAAGGCTTAAAGGCA+AGG 0.588132 tig0024501:-66115 Msa1417640:CDS
TTGCTGTGGAATGTACCGTT+AGG 0.589094 tig0024501:-66930 Msa1417640:CDS
AGGTTGATTTGAAAGACAAG+TGG 0.593616 tig0024501:-64608 Msa1417640:CDS
TTGACAGAATATGGAACCGT+AGG 0.599653 tig0024501:-66849 Msa1417640:CDS
TGACTCTTTGCCAGTTAACG+AGG 0.601690 tig0024501:-66034 Msa1417640:CDS
TGCTTATGTCTACATTGCTG+TGG 0.602167 tig0024501:-66944 Msa1417640:CDS
TTAACATTTAGGCAAGCATG+TGG 0.604145 tig0024501:+65835 None:intergenic
CTTGAAGCAGCAGGTGCAGG+TGG 0.604795 tig0024501:-66904 Msa1417640:CDS
TCTCATATTAGAGTCTCCGA+TGG 0.606409 tig0024501:-66151 Msa1417640:intron
ATTGCAGGCAGGAACATCAA+GGG 0.616311 tig0024501:-65258 Msa1417640:CDS
GTTAGGTACCTTGAAGCAGC+AGG 0.617125 tig0024501:-66913 Msa1417640:CDS
ATTATCGATTTCGGTGACTG+AGG 0.617150 tig0024501:+67137 None:intergenic
ATGGAACTCGACAAAGACAA+TGG 0.617169 tig0024501:-66132 Msa1417640:CDS
TCAGTTCATGCCAAACAGAA+GGG 0.621916 tig0024501:-64514 Msa1417640:three_prime_UTR
TGTCTGATATTGCGTTAGCA+GGG 0.622995 tig0024501:-64354 Msa1417640:three_prime_UTR
TGAAAATGGAAAGGTTAGGG+TGG 0.628807 tig0024501:-67236 Msa1417640:exon
CCTAAATGTTAACAATGATG+AGG 0.630277 tig0024501:-65824 Msa1417640:CDS
CAACCATTATGAGCAGCAGA+AGG 0.630372 tig0024501:+66613 None:intergenic
TGCTGCTTCAAGGTACCTAA+CGG 0.634086 tig0024501:+66915 None:intergenic
ATACAAATAGAATTGGGCAC+AGG 0.635848 tig0024501:+65949 None:intergenic
ATGGATGCTTGAACTTCTCG+AGG 0.640307 tig0024501:-67046 Msa1417640:CDS
TTGCAGGCAGGAACATCAAG+GGG 0.642905 tig0024501:-65257 Msa1417640:CDS
TTCGTTTGCGTAGTCCAGAG+AGG 0.651598 tig0024501:-65223 Msa1417640:CDS
CAACAGAGTGAAGAAAGGAA+GGG 0.656227 tig0024501:+66641 None:intergenic
CGTAGGTTTCAAAACCTGGA+GGG 0.661614 tig0024501:-66832 Msa1417640:CDS
CAGAAGGAAAGCCATTCAAG+AGG 0.668057 tig0024501:-66704 Msa1417640:CDS
TAACATTTAGGCAAGCATGT+GGG 0.675244 tig0024501:+65836 None:intergenic
ACAAGTGGAGAAATTTGAAG+CGG 0.678726 tig0024501:-64593 Msa1417640:CDS
AGACATTGTAAGGTCTGATG+TGG 0.687030 tig0024501:-65923 Msa1417640:CDS
CCGTAGGTTTCAAAACCTGG+AGG 0.690526 tig0024501:-66833 Msa1417640:CDS
TAGTTCTGCTCGTACTCACG+AGG 0.692042 tig0024501:-65737 Msa1417640:intron
TGATGAGGCCAATCTTACGA+AGG 0.699645 tig0024501:-65809 Msa1417640:CDS
TAACGAGGCCCAAAAGAACG+GGG 0.712659 tig0024501:-66019 Msa1417640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTTGAATAGTAAAA+TGG + tig0024501:66740-66759 None:intergenic 10.0%
!!! TTTGTAATTTTTTGTATTTC+TGG - tig0024501:65904-65923 Msa1417640:CDS 15.0%
!! TAAAATAAGCAATAAAATCA+AGG + tig0024501:66306-66325 None:intergenic 15.0%
!! AAATATTATAGTAACTTGAT+TGG + tig0024501:66539-66558 None:intergenic 15.0%
!! TCAGTAAAAAAAAAAAAAGA+AGG + tig0024501:67075-67094 None:intergenic 15.0%
!!! ATAGTATTACTTTTTGAATT+TGG - tig0024501:67160-67179 Msa1417640:CDS 15.0%
!! AAAAACTACTTCTAATTGAT+TGG - tig0024501:64592-64611 Msa1417640:CDS 20.0%
!! AAAACTACTTCTAATTGATT+GGG - tig0024501:64593-64612 Msa1417640:CDS 20.0%
!!! TTTTTAGAGTAAAAAGGAAT+GGG + tig0024501:66218-66237 None:intergenic 20.0%
!!! TTATTGCTTATTTTATGTAG+TGG - tig0024501:66311-66330 Msa1417640:intron 20.0%
!!! TATTGCTTATTTTATGTAGT+GGG - tig0024501:66312-66331 Msa1417640:intron 20.0%
!! AACCAAAAAATATTACGAAA+AGG - tig0024501:66437-66456 Msa1417640:intron 20.0%
!! AAAGGAGAAAGAAAAAAAAA+TGG - tig0024501:66455-66474 Msa1417640:intron 20.0%
!!! ATACAATGTTTTTTCGTTTT+TGG - tig0024501:66576-66595 Msa1417640:intron 20.0%
!! AAAAATGTATGACAAAGTAA+AGG + tig0024501:66629-66648 None:intergenic 20.0%
!! AATTGAAGCATAAATAGTTA+GGG + tig0024501:66765-66784 None:intergenic 20.0%
!!! CTTTTTTCTTTTTTCAGAAT+TGG - tig0024501:66850-66869 Msa1417640:CDS 20.0%
!!! TGAATCAAATTTTGTTTATC+AGG - tig0024501:66981-67000 Msa1417640:CDS 20.0%
!!! TACATGCATAAATTTTTCAT+TGG + tig0024501:67050-67069 None:intergenic 20.0%
!!! TATTACTTTTTGAATTTGGA+AGG - tig0024501:67164-67183 Msa1417640:CDS 20.0%
!!! AGATGTATCTAACTTATTTT+AGG - tig0024501:67331-67350 Msa1417640:five_prime_UTR 20.0%
! AAGAGAAAGGAATTTGAAAA+TGG - tig0024501:64359-64378 Msa1417640:three_prime_UTR 25.0%
! AAAGGAATTTGAAAATGGAA+AGG - tig0024501:64364-64383 Msa1417640:three_prime_UTR 25.0%
! AATTTGAAAATGGAAAGGTT+AGG - tig0024501:64369-64388 Msa1417640:three_prime_UTR 25.0%
! ATTTGAAAATGGAAAGGTTA+GGG - tig0024501:64370-64389 Msa1417640:three_prime_UTR 25.0%
! AACAACAACAACAGAAAAAA+GGG + tig0024501:65035-65054 None:intergenic 25.0%
!!! GTTGTTGTTGTTGTTTATTA+CGG - tig0024501:65046-65065 Msa1417640:intron 25.0%
! AATTTCATTGCAAAATGCAT+AGG - tig0024501:65290-65309 Msa1417640:intron 25.0%
! ATTTCATTGCAAAATGCATA+GGG - tig0024501:65291-65310 Msa1417640:intron 25.0%
!! AACAGGTATTTTTCATAACT+CGG + tig0024501:65399-65418 None:intergenic 25.0%
!!! AAAAGTTCTAAAAAGCAAAC+AGG + tig0024501:65416-65435 None:intergenic 25.0%
! TTGCAATTTATCTGCATTTA+GGG + tig0024501:65994-66013 None:intergenic 25.0%
! TTTGCAATTTATCTGCATTT+AGG + tig0024501:65995-66014 None:intergenic 25.0%
!!! GTTTTTAGAGTAAAAAGGAA+TGG + tig0024501:66219-66238 None:intergenic 25.0%
!!! CTAGTGTTTTTAGAGTAAAA+AGG + tig0024501:66224-66243 None:intergenic 25.0%
! AGAACAAAAAATTACAAGCA+TGG + tig0024501:66276-66295 None:intergenic 25.0%
!! CTCCTTTTCGTAATATTTTT+TGG + tig0024501:66442-66461 None:intergenic 25.0%
! AGAAAAAAAAATGGAGTCAA+TGG - tig0024501:66464-66483 Msa1417640:intron 25.0%
! GAAAAAAAAATGGAGTCAAT+GGG - tig0024501:66465-66484 Msa1417640:intron 25.0%
! AAGTACATACAGTTTAATTC+CGG + tig0024501:66509-66528 None:intergenic 25.0%
! AAAGTAAAGGTAAAAGCAAA+AGG + tig0024501:66616-66635 None:intergenic 25.0%
! CAATTGAAGCATAAATAGTT+AGG + tig0024501:66766-66785 None:intergenic 25.0%
! GATTGTGAAGTAAAATTCTA+GGG + tig0024501:67216-67235 None:intergenic 25.0%
! TGATTGTGAAGTAAAATTCT+AGG + tig0024501:67217-67236 None:intergenic 25.0%
!! TTTTACTTCACAATCATGAA+AGG - tig0024501:67221-67240 Msa1417640:CDS 25.0%
!!! CCTTTAAAACAAAAGTTACT+TGG + tig0024501:67287-67306 None:intergenic 25.0%
!!! CCAAGTAACTTTTGTTTTAA+AGG - tig0024501:67284-67303 Msa1417640:five_prime_UTR 25.0%
! ATAAGTTAGATACATCTTGA+GGG + tig0024501:67328-67347 None:intergenic 25.0%
! AATAAGTTAGATACATCTTG+AGG + tig0024501:67329-67348 None:intergenic 25.0%
TATAGAGAGTGAGCATATTA+AGG - tig0024501:64431-64450 Msa1417640:three_prime_UTR 30.0%
TAACTCCAAAATTGAGATTC+TGG - tig0024501:64814-64833 Msa1417640:intron 30.0%
! CTTTTCCAGAATCTCAATTT+TGG + tig0024501:64822-64841 None:intergenic 30.0%
AAAAAGTTGAACCTCTTGAA+TGG + tig0024501:64919-64938 None:intergenic 30.0%
!!! TCAAGAGGTTCAACTTTTTT+TGG - tig0024501:64920-64939 Msa1417640:intron 30.0%
ACAACAACAACAGAAAAAAG+GGG + tig0024501:65034-65053 None:intergenic 30.0%
CAACAACAACAACAGAAAAA+AGG + tig0024501:65036-65055 None:intergenic 30.0%
TTCACTTAAATGCAATTCTG+TGG - tig0024501:65614-65633 Msa1417640:intron 30.0%
TCTGGCATACAAATAGAATT+GGG + tig0024501:65669-65688 None:intergenic 30.0%
CCTCATCATTGTTAACATTT+AGG + tig0024501:65788-65807 None:intergenic 30.0%
CCTAAATGTTAACAATGATG+AGG - tig0024501:65785-65804 Msa1417640:CDS 30.0%
ATGATGAACATCACTTTGAT+GGG + tig0024501:65832-65851 None:intergenic 30.0%
!!! ATTTCTGGTTGATTCCTTTT+TGG - tig0024501:65919-65938 Msa1417640:CDS 30.0%
!!! TTTCTGGTTGATTCCTTTTT+GGG - tig0024501:65920-65939 Msa1417640:CDS 30.0%
ACTAACAAAGTCTACATTCA+GGG + tig0024501:66023-66042 None:intergenic 30.0%
TCAGGGACTAAATTAACTAA+CGG + tig0024501:66070-66089 None:intergenic 30.0%
TTTGCATATTATCTGCATTC+AGG - tig0024501:66143-66162 Msa1417640:CDS 30.0%
TTGCATATTATCTGCATTCA+GGG - tig0024501:66144-66163 Msa1417640:intron 30.0%
AAGCAATAAAATCAAGGCAT+CGG + tig0024501:66300-66319 None:intergenic 30.0%
!!! TCTTTTTTCAGAATTGGAGA+AGG - tig0024501:66856-66875 Msa1417640:CDS 30.0%
AACTGAAGTAAGTCTAGTAA+AGG - tig0024501:66921-66940 Msa1417640:CDS 30.0%
! AGTAAGTCTAGTAAAGGTAT+TGG - tig0024501:66927-66946 Msa1417640:CDS 30.0%
!!! ACTGCTTTTTTAAAGCTGCA+CGG + tig0024501:64292-64311 None:intergenic 35.0%
TAGAAGAGAAGAGAAGAGAA+AGG - tig0024501:64346-64365 Msa1417640:three_prime_UTR 35.0%
!! CATCTTCCGATTATCGATTT+CGG + tig0024501:64484-64503 None:intergenic 35.0%
GAAGATGAAAATGCTTCTGT+TGG - tig0024501:64497-64516 Msa1417640:three_prime_UTR 35.0%
ATGAAAATGCTTCTGTTGGA+AGG - tig0024501:64501-64520 Msa1417640:three_prime_UTR 35.0%
GTAGACATAAGCAGATTTCA+TGG + tig0024501:64658-64677 None:intergenic 35.0%
! CATGTATCGTGAGTTGAAAA+GGG + tig0024501:64859-64878 None:intergenic 35.0%
!!! AGAGGTTCAACTTTTTTTGG+AGG - tig0024501:64923-64942 Msa1417640:intron 35.0%
!!! AACTTTTTTTGGAGGATGCT+CGG - tig0024501:64931-64950 Msa1417640:intron 35.0%
ATGAAGCACAAGCACATATA+CGG + tig0024501:65189-65208 None:intergenic 35.0%
TATATGTGCTTGTGCTTCAT+AGG - tig0024501:65189-65208 Msa1417640:CDS 35.0%
! AGTGAGAGCCTTATATACTT+TGG - tig0024501:65260-65279 Msa1417640:CDS 35.0%
! GTGAGAGCCTTATATACTTT+GGG - tig0024501:65261-65280 Msa1417640:CDS 35.0%
TCTCTCTCCCAAAGTATATA+AGG + tig0024501:65271-65290 None:intergenic 35.0%
CCTAACCAATGAATGATTCA+TGG + tig0024501:65320-65339 None:intergenic 35.0%
! CCATGAATCATTCATTGGTT+AGG - tig0024501:65317-65336 Msa1417640:intron 35.0%
AAAGACAATGGAAGGCTTAA+AGG - tig0024501:65489-65508 Msa1417640:intron 35.0%
! TACTACTGTTGTTGTTGTTG+AGG - tig0024501:65521-65540 Msa1417640:intron 35.0%
! TGTTGTTGTTGTTGAGGTAA+TGG - tig0024501:65527-65546 Msa1417640:intron 35.0%
ATACAAATAGAATTGGGCAC+AGG + tig0024501:65663-65682 None:intergenic 35.0%
GTCTGGCATACAAATAGAAT+TGG + tig0024501:65670-65689 None:intergenic 35.0%
TTTGTATGCCAGACATTGTA+AGG - tig0024501:65676-65695 Msa1417640:intron 35.0%
!!! TTACTCTGGTTTTCCATTTG+TGG + tig0024501:65735-65754 None:intergenic 35.0%
GTACATTTGCATCTTTACTC+TGG + tig0024501:65749-65768 None:intergenic 35.0%
TAACATTTAGGCAAGCATGT+GGG + tig0024501:65776-65795 None:intergenic 35.0%
TTAACATTTAGGCAAGCATG+TGG + tig0024501:65777-65796 None:intergenic 35.0%
GATGATGAACATCACTTTGA+TGG + tig0024501:65833-65852 None:intergenic 35.0%
! CTATTTGGTTTCATCAAGCT+AGG + tig0024501:65855-65874 None:intergenic 35.0%
!! TTCTGGTTGATTCCTTTTTG+GGG - tig0024501:65921-65940 Msa1417640:CDS 35.0%
AAGTGACTAATTCCCCAAAA+AGG + tig0024501:65936-65955 None:intergenic 35.0%
GACTAACAAAGTCTACATTC+AGG + tig0024501:66024-66043 None:intergenic 35.0%
ACTAACGAAGTCTACATTCA+GGG + tig0024501:66087-66106 None:intergenic 35.0%
! TTTGGTTCATACTCCTTCAA+CGG + tig0024501:66424-66443 None:intergenic 35.0%
AATGGGAAGAGGATAATCTA+AGG - tig0024501:66482-66501 Msa1417640:intron 35.0%
!! AGGTTGATTTGAAAGACAAG+TGG - tig0024501:67001-67020 Msa1417640:CDS 35.0%
ACAAGTGGAGAAATTTGAAG+CGG - tig0024501:67016-67035 Msa1417640:CDS 35.0%
ATATATACAGCCCTTCTGTT+TGG + tig0024501:67108-67127 None:intergenic 35.0%
! TGAATTTGGAAGGTATGTTC+AGG - tig0024501:67174-67193 Msa1417640:CDS 35.0%
TGAAAATGGAAAGGTTAGGG+TGG - tig0024501:64373-64392 Msa1417640:three_prime_UTR 40.0%
! TGGTGTTTGGAATTCGTTCT+TGG - tig0024501:64393-64412 Msa1417640:three_prime_UTR 40.0%
!! ATTATCGATTTCGGTGACTG+AGG + tig0024501:64475-64494 None:intergenic 40.0%
CAGTCACCGAAATCGATAAT+CGG - tig0024501:64475-64494 Msa1417640:three_prime_UTR 40.0%
TGGACAACAACTCATCTTCA+AGG + tig0024501:64527-64546 None:intergenic 40.0%
CCATTCATCGATAGATGCTT+TGG + tig0024501:64547-64566 None:intergenic 40.0%
! CCAAAGCATCTATCGATGAA+TGG - tig0024501:64544-64563 Msa1417640:three_prime_UTR 40.0%
! TTGGGTTAGGGTTTGAAGAA+GGG + tig0024501:64620-64639 None:intergenic 40.0%
! TTTGGGTTAGGGTTTGAAGA+AGG + tig0024501:64621-64640 None:intergenic 40.0%
! CGCTAATAGGATTTGGGTTA+GGG + tig0024501:64632-64651 None:intergenic 40.0%
! TCGCTAATAGGATTTGGGTT+AGG + tig0024501:64633-64652 None:intergenic 40.0%
TGGCATCGCTAATAGGATTT+GGG + tig0024501:64638-64657 None:intergenic 40.0%
ATGGCATCGCTAATAGGATT+TGG + tig0024501:64639-64658 None:intergenic 40.0%
GATTTCATGGCATCGCTAAT+AGG + tig0024501:64645-64664 None:intergenic 40.0%
TGCTTATGTCTACATTGCTG+TGG - tig0024501:64665-64684 Msa1417640:intron 40.0%
ATATTCTGTCAACTGCTTGG+AGG + tig0024501:64752-64771 None:intergenic 40.0%
TCCAAGCAGTTGACAGAATA+TGG - tig0024501:64751-64770 Msa1417640:intron 40.0%
TCCATATTCTGTCAACTGCT+TGG + tig0024501:64755-64774 None:intergenic 40.0%
TTGACAGAATATGGAACCGT+AGG - tig0024501:64760-64779 Msa1417640:intron 40.0%
!! TGAAAAGGGAAGCATCGATT+CGG + tig0024501:64845-64864 None:intergenic 40.0%
! GCATGTATCGTGAGTTGAAA+AGG + tig0024501:64860-64879 None:intergenic 40.0%
CTTCATTGTCTGATACCAGA+AGG - tig0024501:64889-64908 Msa1417640:intron 40.0%
CAACAGAGTGAAGAAAGGAA+GGG + tig0024501:64971-64990 None:intergenic 40.0%
GTGTCAAGTCAACATTGTGT+CGG + tig0024501:65125-65144 None:intergenic 40.0%
GGTCTCCATGAATCATTCAT+TGG - tig0024501:65312-65331 Msa1417640:intron 40.0%
! ATAACTCGGAAAGCGTGAAT+GGG + tig0024501:65385-65404 None:intergenic 40.0%
TCTCATATTAGAGTCTCCGA+TGG - tig0024501:65458-65477 Msa1417640:intron 40.0%
TTGTCTTTGTCGAGTTCCAT+CGG + tig0024501:65477-65496 None:intergenic 40.0%
ATGGAACTCGACAAAGACAA+TGG - tig0024501:65477-65496 Msa1417640:intron 40.0%
AACTCGACAAAGACAATGGA+AGG - tig0024501:65481-65500 Msa1417640:intron 40.0%
! AGAGTCAATTTTGCTACAGC+TGG + tig0024501:65562-65581 None:intergenic 40.0%
! AAGTGAATCCCCGTTCTTTT+GGG + tig0024501:65601-65620 None:intergenic 40.0%
! TAAGTGAATCCCCGTTCTTT+TGG + tig0024501:65602-65621 None:intergenic 40.0%
ACATCAGACCTTACAATGTC+TGG + tig0024501:65687-65706 None:intergenic 40.0%
AGACATTGTAAGGTCTGATG+TGG - tig0024501:65686-65705 Msa1417640:intron 40.0%
GGCAATTGAAGACACAAATC+AGG - tig0024501:65707-65726 Msa1417640:intron 40.0%
TGAGTACGAGCAGAACTATT+TGG + tig0024501:65870-65889 None:intergenic 40.0%
! TAGTCACTTTAGTCCCTGAA+TGG - tig0024501:65947-65966 Msa1417640:CDS 40.0%
! AGTCACTTTAGTCCCTGAAT+GGG - tig0024501:65948-65967 Msa1417640:CDS 40.0%
GACTAACGAAGTCTACATTC+AGG + tig0024501:66088-66107 None:intergenic 40.0%
! AGTCACTTTAGTCCCTGAAT+GGG + tig0024501:65948-65967 None:intergenic 40.0%
! TAGTCACTTTAGTCCCTGAA+TGG + tig0024501:65947-65966 None:intergenic 40.0%
AAAATGGAGTCAATGGGAAG+AGG - tig0024501:66471-66490 Msa1417640:intron 40.0%
GAAGAGGATAATCTAAGGAC+CGG - tig0024501:66487-66506 Msa1417640:intron 40.0%
GGTAAAAGCAAAAGGAGATG+AGG + tig0024501:66608-66627 None:intergenic 40.0%
TCAGAATTGGAGAAGGAAAC+TGG - tig0024501:66863-66882 Msa1417640:CDS 40.0%
ATCAGTTCATGCCAAACAGA+AGG - tig0024501:67094-67113 Msa1417640:CDS 40.0%
TCAGTTCATGCCAAACAGAA+GGG - tig0024501:67095-67114 Msa1417640:CDS 40.0%
AACGCAATATCAGACACTAC+TGG + tig0024501:67251-67270 None:intergenic 40.0%
TGTCTGATATTGCGTTAGCA+GGG - tig0024501:67255-67274 Msa1417640:five_prime_UTR 40.0%
CTGCTGGATCGATTGGAATT+TGG + tig0024501:64323-64342 None:intergenic 45.0%
ATGGATGCTTGAACTTCTCG+AGG - tig0024501:64563-64582 Msa1417640:exon 45.0%
TTGCTGTGGAATGTACCGTT+AGG - tig0024501:64679-64698 Msa1417640:intron 45.0%
! TGCTGCTTCAAGGTACCTAA+CGG + tig0024501:64697-64716 None:intergenic 45.0%
CTGCTTGGAGGTAGTGTTTA+GGG + tig0024501:64740-64759 None:intergenic 45.0%
ACTGCTTGGAGGTAGTGTTT+AGG + tig0024501:64741-64760 None:intergenic 45.0%
GAACCGTAGGTTTCAAAACC+TGG - tig0024501:64773-64792 Msa1417640:intron 45.0%
!!! CCTCCAGGTTTTGAAACCTA+CGG + tig0024501:64779-64798 None:intergenic 45.0%
CGTAGGTTTCAAAACCTGGA+GGG - tig0024501:64777-64796 Msa1417640:intron 45.0%
GCAAATAACTTCTTCCCTCC+AGG + tig0024501:64794-64813 None:intergenic 45.0%
CTCTTGAATGGCTTTCCTTC+TGG + tig0024501:64907-64926 None:intergenic 45.0%
CAGAAGGAAAGCCATTCAAG+AGG - tig0024501:64905-64924 Msa1417640:intron 45.0%
GATGCTCGGAATAACCTTGT+AGG - tig0024501:64945-64964 Msa1417640:intron 45.0%
GCAACAGAGTGAAGAAAGGA+AGG + tig0024501:64972-64991 None:intergenic 45.0%
AGCAGCAACAGAGTGAAGAA+AGG + tig0024501:64976-64995 None:intergenic 45.0%
CAACCATTATGAGCAGCAGA+AGG + tig0024501:64999-65018 None:intergenic 45.0%
CTTCTGCTGCTCATAATGGT+TGG - tig0024501:64997-65016 Msa1417640:intron 45.0%
AAAGCGTGAATGGGGATTCA+GGG + tig0024501:65376-65395 None:intergenic 45.0%
! TAACTCGGAAAGCGTGAATG+GGG + tig0024501:65384-65403 None:intergenic 45.0%
! CATAACTCGGAAAGCGTGAA+TGG + tig0024501:65386-65405 None:intergenic 45.0%
CAATGGAAGGCTTAAAGGCA+AGG - tig0024501:65494-65513 Msa1417640:intron 45.0%
TGACTCTTTGCCAGTTAACG+AGG - tig0024501:65575-65594 Msa1417640:intron 45.0%
! TTCTTTTGGGCCTCGTTAAC+TGG + tig0024501:65588-65607 None:intergenic 45.0%
GTTAACGAGGCCCAAAAGAA+CGG - tig0024501:65588-65607 Msa1417640:intron 45.0%
TTAACGAGGCCCAAAAGAAC+GGG - tig0024501:65589-65608 Msa1417640:intron 45.0%
CACAAATCAGGAGCCACAAA+TGG - tig0024501:65719-65738 Msa1417640:intron 45.0%
TTTAGGCAAGCATGTGGGTT+GGG + tig0024501:65771-65790 None:intergenic 45.0%
ATTTAGGCAAGCATGTGGGT+TGG + tig0024501:65772-65791 None:intergenic 45.0%
TGATGAGGCCAATCTTACGA+AGG - tig0024501:65800-65819 Msa1417640:CDS 45.0%
GGAAGTTGCCTTCGTAAGAT+TGG + tig0024501:65811-65830 None:intergenic 45.0%
GACGAATCGTTTCCCATTCA+GGG + tig0024501:65963-65982 None:intergenic 45.0%
TGACGAATCGTTTCCCATTC+AGG + tig0024501:65964-65983 None:intergenic 45.0%
ACTAACGGAGTCTGCATTCA+GGG + tig0024501:66055-66074 None:intergenic 45.0%
AACTAACGGAGTCTGCATTC+AGG + tig0024501:66056-66075 None:intergenic 45.0%
TCCCTGAAATTGACCATGCA+GGG - tig0024501:66115-66134 Msa1417640:CDS 45.0%
TCCCTGCATGGTCAATTTCA+GGG + tig0024501:66119-66138 None:intergenic 45.0%
TATGCAAAACAGTCCCTGCA+TGG + tig0024501:66131-66150 None:intergenic 45.0%
! GTCACTTTAGTCCCTGAATG+GGG + tig0024501:65949-65968 None:intergenic 45.0%
TAGTGGGATGATTCGATCGA+TGG - tig0024501:66328-66347 Msa1417640:intron 45.0%
!! TGATTCGATCGATGGATTGC+AGG - tig0024501:66336-66355 Msa1417640:intron 45.0%
ATTGCAGGCAGGAACATCAA+GGG - tig0024501:66351-66370 Msa1417640:intron 45.0%
GAAGAAGCTTTCTCCGTTGA+AGG - tig0024501:66408-66427 Msa1417640:intron 45.0%
! TGGAGAAATTTGAAGCGGTC+TGG - tig0024501:67021-67040 Msa1417640:CDS 45.0%
GTGTCTGATATTGCGTTAGC+AGG - tig0024501:67254-67273 Msa1417640:five_prime_UTR 45.0%
TCTAACGCTGCTGGATCGAT+TGG + tig0024501:64330-64349 None:intergenic 50.0%
CTCTTCTCTTCTAACGCTGC+TGG + tig0024501:64339-64358 None:intergenic 50.0%
GGAAAGGTTAGGGTGGTGTT+TGG - tig0024501:64380-64399 Msa1417640:three_prime_UTR 50.0%
GTTAGGTACCTTGAAGCAGC+AGG - tig0024501:64696-64715 Msa1417640:intron 50.0%
CCGTAGGTTTCAAAACCTGG+AGG - tig0024501:64776-64795 Msa1417640:intron 50.0%
GAAGAAAGGAAGGGCCTACA+AGG + tig0024501:64962-64981 None:intergenic 50.0%
GCTCCTTCTGCTGCTCATAA+TGG - tig0024501:64993-65012 Msa1417640:intron 50.0%
!! CATTGTGTCGGTGTCAGACA+TGG + tig0024501:65113-65132 None:intergenic 50.0%
! GAAAGCGTGAATGGGGATTC+AGG + tig0024501:65377-65396 None:intergenic 50.0%
TAACGAGGCCCAAAAGAACG+GGG - tig0024501:65590-65609 Msa1417640:intron 50.0%
! TAGTTCTGCTCGTACTCACG+AGG - tig0024501:65872-65891 Msa1417640:CDS 50.0%
GTCCCTGAAATTGACCATGC+AGG - tig0024501:66114-66133 Msa1417640:CDS 50.0%
GTCCCTGCATGGTCAATTTC+AGG + tig0024501:66120-66139 None:intergenic 50.0%
TGACGAATCGTTCCCCATTC+AGG - tig0024501:66174-66193 Msa1417640:intron 50.0%
GACGAATCGTTCCCCATTCA+GGG - tig0024501:66175-66194 Msa1417640:intron 50.0%
GATTGCAGGCAGGAACATCA+AGG - tig0024501:66350-66369 Msa1417640:intron 50.0%
TTGCAGGCAGGAACATCAAG+GGG - tig0024501:66352-66371 Msa1417640:intron 50.0%
TTCGTTTGCGTAGTCCAGAG+AGG - tig0024501:66386-66405 Msa1417640:intron 50.0%
GGAGAAAGCTTCTTCCTCTC+TGG + tig0024501:66403-66422 None:intergenic 50.0%
TACCTTGAAGCAGCAGGTGC+AGG - tig0024501:64702-64721 Msa1417640:intron 55.0%
!! TCGATCGATGGATTGCAGGC+AGG - tig0024501:66340-66359 Msa1417640:intron 55.0%
! CACCTGCACCTGCTGCTTCA+AGG + tig0024501:64707-64726 None:intergenic 60.0%
CTTGAAGCAGCAGGTGCAGG+TGG - tig0024501:64705-64724 Msa1417640:CDS 60.0%
GCGTGAATGGGGATTCAGGG+AGG + tig0024501:65373-65392 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0024501 gene 64245 67386 64245 ID=Msa1417640;Name=Msa1417640
tig0024501 mRNA 64245 67386 64245 ID=Msa1417640-mRNA-1;Parent=Msa1417640;Name=Msa1417640-mRNA-1;_AED=0.24;_eAED=0.24;_QI=133|1|1|1|1|1|5|333|453
tig0024501 exon 64245 64628 64245 ID=Msa1417640-mRNA-1:exon:23585;Parent=Msa1417640-mRNA-1
tig0024501 exon 64704 64764 64704 ID=Msa1417640-mRNA-1:exon:23584;Parent=Msa1417640-mRNA-1
tig0024501 exon 65113 65300 65113 ID=Msa1417640-mRNA-1:exon:23583;Parent=Msa1417640-mRNA-1
tig0024501 exon 65738 66162 65738 ID=Msa1417640-mRNA-1:exon:23582;Parent=Msa1417640-mRNA-1
tig0024501 exon 66617 67386 66617 ID=Msa1417640-mRNA-1:exon:23581;Parent=Msa1417640-mRNA-1
tig0024501 five_prime_UTR 67254 67386 67254 ID=Msa1417640-mRNA-1:five_prime_utr;Parent=Msa1417640-mRNA-1
tig0024501 CDS 66617 67253 66617 ID=Msa1417640-mRNA-1:cds;Parent=Msa1417640-mRNA-1
tig0024501 CDS 65738 66162 65738 ID=Msa1417640-mRNA-1:cds;Parent=Msa1417640-mRNA-1
tig0024501 CDS 65113 65300 65113 ID=Msa1417640-mRNA-1:cds;Parent=Msa1417640-mRNA-1
tig0024501 CDS 64704 64764 64704 ID=Msa1417640-mRNA-1:cds;Parent=Msa1417640-mRNA-1
tig0024501 CDS 64578 64628 64578 ID=Msa1417640-mRNA-1:cds;Parent=Msa1417640-mRNA-1
tig0024501 three_prime_UTR 64245 64577 64245 ID=Msa1417640-mRNA-1:three_prime_utr;Parent=Msa1417640-mRNA-1
Gene Sequence

>Msa1417640

ATGGAAAGGTTAGGGTGGTGTTTGGAATTCGTTCTTGGACATTCTTCAACAAGTATAGAGAGTGAGCATATTAAGGAAATCTTGAAACTAAGCTCCTCAGTCACCGAAATCGATAATCGGAAGATGAAAATGCTTCTGTTGGAAGGCCTTGAAGATGAGTTGTTGTCCAAAGCATCTATCGATGAATGGATGCTTGAACTTCTCGAGGTATTCGAAAAACTACTTCTAATTGATTGGGACCCTTCTTCAAACCCTAACCCAAATCCTATTAGCGATGCCATGAAATCTGCTTATGTCTACATTGCTGTGGAATGTACCGTTAGGTACCTTGAAGCAGCAGGTGCAGGTGGTATATCTAACCCTAAACACTACCTCCAAGCAGTTGACAGAATATGGAACCGTAGGTTTCAAAACCTGGAGGGAAGAAGTTATTTGCTAACTCCAAAATTGAGATTCTGGAAAAGCCGAATCGATGCTTCCCTTTTCAACTCACGATACATGCTCAAACTTGCTTCATTGTCTGATACCAGAAGGAAAGCCATTCAAGAGGTTCAACTTTTTTTGGAGGATGCTCGGAATAACCTTGTAGGCCCTTCCTTTCTTCACTCTGTTGCTGCTCCTTCTGCTGCTCATAATGAGTCTCCGATGGAACTCGACAAAGACAATGGAAGGCTTAAAGGCAAGGCTTCTACTACTGTTGTTGTTGTTGAGGTAATGGCTACATCAACCAGCTGTAGCAAAATTGACTCTTTGCCAGTTAACGAGGCCCAAAAGAACGGGGATTCACTTAAATGCAATTCTGTGGAGTTCAAAACATTTGCGAACGATCCTGTGCCCAATTCTATTTGTATGCCAGACATTGTAAGGTCTGATGTGGCAATTGAAGACACAAATCAGGAGCCACAAATGGAAAACCAGAGTAAAGATGCAAATGTACCCAACCCACATGCTTGCCTAAATGTTAACAATGATGAGGCCAATCTTACGAAGGCAACTTCCCATCAAAGTGATGTTCATCATCCTAGCTTGATGAAACCAAATAGTTCTGCTCGTACTCACGAGTGGGATGATTCGATCGATGGATTGCAGGCAGGAACATCAAGGGGCACAAGTAGAATTCGTTTGCGTAGTCCAGAGAGGAAGAAGCTTTCTCCGTTGAAGGAGTATGAACCAAAAAATATTACGAAAAGGAGAAAGAAAAAAAAATGGAGTCAATGGGAAGAGGATAATCTAAGGACCGGAATTAAACTAATTGGAGAAGGAAACTGGAAATCAATTCTTCGTTCTTATGAATTTGACGAAAGAACTGAAGTTGATTTGAAAGACAAGTGGAGAAATTTGAAGCGGTCTGGATGCCAATGA
Protein sequence

>Msa1417640

MERLGWCLEFVLGHSSTSIESEHIKEILKLSSSVTEIDNRKMKMLLLEGLEDELLSKASIDEWMLELLEVFEKLLLIDWDPSSNPNPNPISDAMKSAYVYIAVECTVRYLEAAGAGGISNPKHYLQAVDRIWNRRFQNLEGRSYLLTPKLRFWKSRIDASLFNSRYMLKLASLSDTRRKAIQEVQLFLEDARNNLVGPSFLHSVAAPSAAHNESPMELDKDNGRLKGKASTTVVVVEVMATSTSCSKIDSLPVNEAQKNGDSLKCNSVEFKTFANDPVPNSICMPDIVRSDVAIEDTNQEPQMENQSKDANVPNPHACLNVNNDEANLTKATSHQSDVHHPSLMKPNSSARTHEWDDSIDGLQAGTSRGTSRIRLRSPERKKLSPLKEYEPKNITKRRKKKKWSQWEEDNLRTGIKLIGEGNWKSILRSYEFDERTEVDLKDKWRNLKRSGCQ*