AlfalfaGEDB | Msa1453380

M. sativa cultivar zhongmu-4 / Msa1453380

KEGG: K02942
GO: GO:0001932 GO:0001934 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009893 GO:0009987 GO:0010467 GO:0010562 GO:0010604 GO:0015934 GO:0016020 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022625 GO:0022626 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032991 GO:0033674 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042788 GO:0043043 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0098772 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453380	sp\|Q8LCW9\|RLA11_ARATH	52.679	112	51	1	2	113	3	112	2.74e-25	94.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453380	G7L532	95.614	114	4	1	1	114	1	113	1.11e-66	206

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027590	Msa1453380	0.815852	7.474790e-52	-8.615850e-47
Msa1453380	Msa1465700	0.833320	5.707788e-56	-8.615850e-47
Msa0621190	Msa1453380	0.802411	5.681453e-49	-8.615850e-47
Msa0168320	Msa1453380	0.837734	4.364033e-57	-8.615850e-47
Msa0177940	Msa1453380	0.813457	2.535339e-51	-8.615850e-47
Msa0192300	Msa1453380	0.803233	3.844881e-49	-8.615850e-47
Msa1235950	Msa1453380	0.816160	6.382511e-52	-8.615850e-47
Msa1235980	Msa1453380	0.820976	5.160360e-53	-8.615850e-47
Msa1317390	Msa1453380	0.802282	6.041925e-49	-8.615850e-47
Msa0377520	Msa1453380	0.815066	1.118077e-51	-8.615850e-47
Msa0382670	Msa1453380	0.839005	2.052246e-57	-8.615850e-47
Msa0301250	Msa1453380	0.809803	1.579477e-50	-8.615850e-47
Msa1206240	Msa1453380	0.836534	8.846535e-57	-8.615850e-47
Msa0929680	Msa1453380	0.823125	1.639326e-53	-8.615850e-47
Msa0929700	Msa1453380	0.822414	2.399340e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453380	MtrunA17_Chr7g0276131	95.614	114	4	1	1	114	1	113	2.14e-70	206
Msa1453380	MtrunA17_Chr8g0345951	56.637	113	44	1	1	113	1	108	2.89e-32	109
Msa1453380	MtrunA17_Chr4g0013621	55.752	113	47	1	1	113	1	110	2.17e-24	89.4
Msa1453380	MtrunA17_Chr3g0127831	55.752	113	47	1	1	113	1	110	3.78e-22	84.0
Msa1453380	MtrunA17_Chr2g0311301	54.867	113	48	1	1	113	1	110	3.78e-22	84.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453380	AT5G24510.1	50.435	115	51	2	1	113	1	111	3.07e-31	107
Msa1453380	AT1G01100.4	52.679	112	51	1	2	113	3	112	2.78e-26	94.7
Msa1453380	AT1G01100.1	52.679	112	51	1	2	113	3	112	2.78e-26	94.7
Msa1453380	AT1G01100.2	52.679	112	51	1	2	113	3	112	2.78e-26	94.7
Msa1453380	AT4G00810.1	55.357	112	49	1	2	113	3	113	3.02e-26	94.7
Msa1453380	AT4G00810.2	55.357	112	49	1	2	113	3	113	3.02e-26	94.7
Msa1453380	AT5G47700.1	57.143	112	47	1	2	113	3	113	3.37e-26	94.4
Msa1453380	AT5G47700.2	57.143	112	47	1	2	113	3	113	3.37e-26	94.4
Msa1453380	AT1G01100.3	47.321	112	41	1	2	113	3	96	4.04e-26	94.0

Find 63 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAAACATGCATTATTTAA+AGG	0.114451	tig0034656:-29359	Msa1453380:five_prime_UTR
TCTCCTTGGGCTTTATGTTT+TGG	0.214678	tig0034656:+28910	None:intergenic
ATAGAAGTATGGTTCTAAAA+TGG	0.235846	tig0034656:-28934	Msa1453380:five_prime_UTR
AGAAGAGAGTGACGAGGAAA+TGG	0.262947	tig0034656:-28380	Msa1453380:CDS
AATAAATATGCAAATAAATA+TGG	0.266625	tig0034656:-29304	Msa1453380:five_prime_UTR
TCGTCTCCGATCTCAATTTC+AGG	0.281808	tig0034656:-28798	Msa1453380:intron
TCTATTAGCTCTTTGAATAC+TGG	0.295098	tig0034656:-28305	Msa1453380:three_prime_UTR
AGCAGCAGCTGGAGCAGCAG+CGG	0.297502	tig0034656:+28429	None:intergenic
CTCTCTTCTTCCTCTTGCTC+TGG	0.301098	tig0034656:+28394	None:intergenic
AAATGATATGATTGCTTGCT+TGG	0.329661	tig0034656:-28101	Msa1453380:three_prime_UTR
TTCTTCTCAGCAGCAGCAGC+TGG	0.337075	tig0034656:+28418	None:intergenic
GCTGCTGGAGGTGGTGCTCC+TGG	0.339216	tig0034656:-28496	Msa1453380:CDS
ATTCGGCTTGTTCGATGAAT+AGG	0.347729	tig0034656:-28356	Msa1453380:exon
TTCTAATGCTGATTCTCCTT+GGG	0.348945	tig0034656:+28897	None:intergenic
AGGTTGATGTTGAATCCTTC+TGG	0.358591	tig0034656:-28576	Msa1453380:CDS
TTTGCTATCACTTGAGGATT+CGG	0.364443	tig0034656:-28261	Msa1453380:three_prime_UTR
CCACCACCTGATGCTGCAAC+TGG	0.368389	tig0034656:+28454	None:intergenic
GCTGCTCCAGTTGCAGCATC+AGG	0.384530	tig0034656:-28460	Msa1453380:CDS
ATTCTAATGCTGATTCTCCT+TGG	0.388060	tig0034656:+28896	None:intergenic
GCAGCCGCAACAGCTGCTCC+AGG	0.413578	tig0034656:+28478	None:intergenic
GATTTGATTGCCAGTGCTGC+TGG	0.422177	tig0034656:-28511	Msa1453380:CDS
TAGAAGTATGGTTCTAAAAT+GGG	0.425076	tig0034656:-28933	Msa1453380:five_prime_UTR
GAAGAGAGTGACGAGGAAAT+GGG	0.432201	tig0034656:-28379	Msa1453380:CDS
CTAATTTCACAAAACATTTG+CGG	0.434513	tig0034656:-28853	Msa1453380:five_prime_UTR
TTCTTATGATCCTAATGGAT+TGG	0.442659	tig0034656:-27961	Msa1453380:three_prime_UTR
AGCACCACCTCCAGCAGCAC+TGG	0.442836	tig0034656:+28501	None:intergenic
CAACAAAGTTGTGATGTTGT+CGG	0.447259	tig0034656:+28609	None:intergenic
AAAATAACAAAACATTTACT+TGG	0.447274	tig0034656:+28238	None:intergenic
ATGAACCGAACCAATCCATT+AGG	0.451768	tig0034656:+27951	None:intergenic
TTTACTCAAATCACTGCAAA+AGG	0.453141	tig0034656:-29012	Msa1453380:five_prime_UTR
ATGATCCTAATGGATTGGTT+CGG	0.464603	tig0034656:-27956	Msa1453380:three_prime_UTR
TCCGGTATAGAATAGAAGTA+TGG	0.467577	tig0034656:-28945	Msa1453380:five_prime_UTR
CAAATCACTGCAAAAGGAAA+AGG	0.481251	tig0034656:-29006	Msa1453380:five_prime_UTR
GTGATGTTGTCGGCAGTTGC+TGG	0.481555	tig0034656:+28619	None:intergenic
AATGATATGATTGCTTGCTT+GGG	0.483094	tig0034656:-28100	Msa1453380:three_prime_UTR
CGGAGACGACGAGAAGAGTA+AGG	0.491125	tig0034656:+28812	None:intergenic
ATTGCCAGTGCTGCTGGAGG+TGG	0.492216	tig0034656:-28505	Msa1453380:CDS
CCAGTTGCAGCATCAGGTGG+TGG	0.493184	tig0034656:-28454	Msa1453380:CDS
TTAGCTCTTTGAATACTGGT+TGG	0.495732	tig0034656:-28301	Msa1453380:three_prime_UTR
GGATTCAACATCAACCTTTG+CGG	0.496398	tig0034656:+28582	None:intergenic
CTGGAATGTTGTCATCTTCG+AGG	0.500813	tig0034656:+28638	None:intergenic
AGTGACGAGGAAATGGGATT+CGG	0.504376	tig0034656:-28373	Msa1453380:CDS
ACCATACTTCTATTCTATAC+CGG	0.531946	tig0034656:+28944	None:intergenic
TGAGAAGAAACCAGAGCAAG+AGG	0.553065	tig0034656:-28404	Msa1453380:CDS
GCAAGTTTAGCGAATAAAGC+GGG	0.558481	tig0034656:+28553	None:intergenic
TGTCATCTTCGAGGATGAGA+AGG	0.559827	tig0034656:+28647	None:intergenic
TGCTCCTGGAGCAGCTGTTG+CGG	0.561254	tig0034656:-28482	Msa1453380:CDS
GCTCCAGTTGCAGCATCAGG+TGG	0.561855	tig0034656:-28457	Msa1453380:CDS
GCTCAAGTCGAGTTTCGAGT+TGG	0.565167	tig0034656:+29102	None:intergenic
AACTTTGTTGAAATCCGCAA+AGG	0.566474	tig0034656:-28596	Msa1453380:CDS
AGCGAATAAAGCGGGCCAGA+AGG	0.567952	tig0034656:+28561	None:intergenic
AGCAAGTTTAGCGAATAAAG+CGG	0.583424	tig0034656:+28552	None:intergenic
CACGTACACATGTGTGTGTG+TGG	0.591738	tig0034656:+29206	None:intergenic
GTGTTACCTGAAATTGAGAT+CGG	0.599697	tig0034656:+28792	None:intergenic
GTCATCTTCGAGGATGAGAA+GGG	0.616441	tig0034656:+28648	None:intergenic
ATTAAAATCAGCGAACAACT+CGG	0.622623	tig0034656:-29044	Msa1453380:five_prime_UTR
GGGCCAAAACATAAAGCCCA+AGG	0.625357	tig0034656:-28913	Msa1453380:five_prime_UTR
GGTTACGAAGAGTAGCAGTC+CGG	0.629569	tig0034656:-28963	Msa1453380:five_prime_UTR
TTTGTGAAATTAGGGTTTCG+GGG	0.638313	tig0034656:+28863	None:intergenic
TTGATTGCCAGTGCTGCTGG+AGG	0.642263	tig0034656:-28508	Msa1453380:CDS
TGTAGTGTGAAAATGTCGCT+CGG	0.684963	tig0034656:-28691	Msa1453380:intron
AGACGACGAGAAGAGTAAGG+AGG	0.685904	tig0034656:+28815	None:intergenic
AGAGGAAGAAGAGAGTGACG+AGG	0.692585	tig0034656:-28386	Msa1453380:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAATATGCAAATAAATA+TGG	-	tig0034656:27988-28007	Msa1453380:three_prime_UTR	10.0%
!!	AATAAATATGCAAATAAATA+TGG	-	tig0034656:27988-28007	Msa1453380:three_prime_UTR	10.0%
!!!	TCCTTTTTGAAAAAAAAAAA+TGG	+	tig0034656:28128-28147	None:intergenic	15.0%
!!!	ATTTTTTTTTTTCAAAAAGG+AGG	-	tig0034656:28127-28146	Msa1453380:three_prime_UTR	15.0%
!!	AAAATAACAAAACATTTACT+TGG	+	tig0034656:29057-29076	None:intergenic	15.0%
!!!	TCATTATCTTTTATCTTTTT+AGG	-	tig0034656:29252-29271	Msa1453380:five_prime_UTR	15.0%
!!!	TCCTTTTTGAAAAAAAAAAA+TGG	+	tig0034656:28128-28147	None:intergenic	15.0%
!!!	ATTTTTTTTTTTCAAAAAGG+AGG	-	tig0034656:28127-28146	Msa1453380:three_prime_UTR	15.0%
!!	AAAATAACAAAACATTTACT+TGG	+	tig0034656:29057-29076	None:intergenic	15.0%
!!!	TCATTATCTTTTATCTTTTT+AGG	-	tig0034656:29252-29271	Msa1453380:five_prime_UTR	15.0%
!!	CATAAACATGCATTATTTAA+AGG	-	tig0034656:27933-27952	Msa1453380:three_prime_UTR	20.0%
!!!	GCCATTTTTTTTTTTCAAAA+AGG	-	tig0034656:28124-28143	Msa1453380:three_prime_UTR	20.0%
!!	AAAGGAACAAAAAAGAAAAA+AGG	-	tig0034656:28304-28323	Msa1453380:three_prime_UTR	20.0%
!!!	TTTGTTTTTGTTATTCGAAT+TGG	-	tig0034656:28527-28546	Msa1453380:CDS	20.0%
!!	CATAAACATGCATTATTTAA+AGG	-	tig0034656:27933-27952	Msa1453380:three_prime_UTR	20.0%
!!!	GCCATTTTTTTTTTTCAAAA+AGG	-	tig0034656:28124-28143	Msa1453380:three_prime_UTR	20.0%
!!	AAAGGAACAAAAAAGAAAAA+AGG	-	tig0034656:28304-28323	Msa1453380:three_prime_UTR	20.0%
!!!	TTTGTTTTTGTTATTCGAAT+TGG	-	tig0034656:28527-28546	Msa1453380:CDS	20.0%
!!	CTGTTTTGTATTATTGCTTT+TGG	+	tig0034656:28046-28065	None:intergenic	25.0%
!	GAACAAAAAAGAAAAAAGGA+GGG	-	tig0034656:28308-28327	Msa1453380:three_prime_UTR	25.0%
!!!	ATAGAAGTATGGTTCTAAAA+TGG	-	tig0034656:28358-28377	Msa1453380:CDS	25.0%
!!!	TAGAAGTATGGTTCTAAAAT+GGG	-	tig0034656:28359-28378	Msa1453380:CDS	25.0%
!!	GCAAATGTTTTGTGAAATTA+GGG	+	tig0034656:28440-28459	None:intergenic	25.0%
!	CTAATTTCACAAAACATTTG+CGG	-	tig0034656:28439-28458	Msa1453380:CDS	25.0%
!	TTGCTGAGAAGAAAAATATT+AGG	-	tig0034656:28758-28777	Msa1453380:intron	25.0%
!	TGCTGAGAAGAAAAATATTA+GGG	-	tig0034656:28759-28778	Msa1453380:intron	25.0%
!!!	TTTTATTTTGCTATCACTTG+AGG	-	tig0034656:29025-29044	Msa1453380:five_prime_UTR	25.0%
!!	AGATTTTCTTATGATCCTAA+TGG	-	tig0034656:29326-29345	Msa1453380:five_prime_UTR	25.0%
!!	CTGTTTTGTATTATTGCTTT+TGG	+	tig0034656:28046-28065	None:intergenic	25.0%
!	GAACAAAAAAGAAAAAAGGA+GGG	-	tig0034656:28308-28327	Msa1453380:three_prime_UTR	25.0%
!!!	ATAGAAGTATGGTTCTAAAA+TGG	-	tig0034656:28358-28377	Msa1453380:CDS	25.0%
!!!	TAGAAGTATGGTTCTAAAAT+GGG	-	tig0034656:28359-28378	Msa1453380:CDS	25.0%
!!	GCAAATGTTTTGTGAAATTA+GGG	+	tig0034656:28440-28459	None:intergenic	25.0%
!	CTAATTTCACAAAACATTTG+CGG	-	tig0034656:28439-28458	Msa1453380:CDS	25.0%
!	TTGCTGAGAAGAAAAATATT+AGG	-	tig0034656:28758-28777	Msa1453380:intron	25.0%
!	TGCTGAGAAGAAAAATATTA+GGG	-	tig0034656:28759-28778	Msa1453380:intron	25.0%
!!!	TTTTATTTTGCTATCACTTG+AGG	-	tig0034656:29025-29044	Msa1453380:five_prime_UTR	25.0%
!!	AGATTTTCTTATGATCCTAA+TGG	-	tig0034656:29326-29345	Msa1453380:five_prime_UTR	25.0%
	ATTAAAATCAGCGAACAACT+CGG	-	tig0034656:28248-28267	Msa1453380:three_prime_UTR	30.0%
	TTTACTCAAATCACTGCAAA+AGG	-	tig0034656:28280-28299	Msa1453380:three_prime_UTR	30.0%
	GGAACAAAAAAGAAAAAAGG+AGG	-	tig0034656:28307-28326	Msa1453380:three_prime_UTR	30.0%
	ACCATACTTCTATTCTATAC+CGG	+	tig0034656:28351-28370	None:intergenic	30.0%
!	TTTTGTGAAATTAGGGTTTC+GGG	+	tig0034656:28433-28452	None:intergenic	30.0%
!	GTTTTGTGAAATTAGGGTTT+CGG	+	tig0034656:28434-28453	None:intergenic	30.0%
!	CGCAAATGTTTTGTGAAATT+AGG	+	tig0034656:28441-28460	None:intergenic	30.0%
	AAATCAAGATCTGATCTGTT+AGG	+	tig0034656:28578-28597	None:intergenic	30.0%
!	TCTATTAGCTCTTTGAATAC+TGG	-	tig0034656:28987-29006	Msa1453380:five_prime_UTR	30.0%
	AAATGATATGATTGCTTGCT+TGG	-	tig0034656:29191-29210	Msa1453380:five_prime_UTR	30.0%
	AATGATATGATTGCTTGCTT+GGG	-	tig0034656:29192-29211	Msa1453380:five_prime_UTR	30.0%
!	TTCTTATGATCCTAATGGAT+TGG	-	tig0034656:29331-29350	Msa1453380:five_prime_UTR	30.0%
	ATTAAAATCAGCGAACAACT+CGG	-	tig0034656:28248-28267	Msa1453380:three_prime_UTR	30.0%
	TTTACTCAAATCACTGCAAA+AGG	-	tig0034656:28280-28299	Msa1453380:three_prime_UTR	30.0%
	GGAACAAAAAAGAAAAAAGG+AGG	-	tig0034656:28307-28326	Msa1453380:three_prime_UTR	30.0%
	ACCATACTTCTATTCTATAC+CGG	+	tig0034656:28351-28370	None:intergenic	30.0%
!	TTTTGTGAAATTAGGGTTTC+GGG	+	tig0034656:28433-28452	None:intergenic	30.0%
!	GTTTTGTGAAATTAGGGTTT+CGG	+	tig0034656:28434-28453	None:intergenic	30.0%
!	CGCAAATGTTTTGTGAAATT+AGG	+	tig0034656:28441-28460	None:intergenic	30.0%
	AAATCAAGATCTGATCTGTT+AGG	+	tig0034656:28578-28597	None:intergenic	30.0%
!	TCTATTAGCTCTTTGAATAC+TGG	-	tig0034656:28987-29006	Msa1453380:five_prime_UTR	30.0%
	AAATGATATGATTGCTTGCT+TGG	-	tig0034656:29191-29210	Msa1453380:five_prime_UTR	30.0%
	AATGATATGATTGCTTGCTT+GGG	-	tig0034656:29192-29211	Msa1453380:five_prime_UTR	30.0%
!	TTCTTATGATCCTAATGGAT+TGG	-	tig0034656:29331-29350	Msa1453380:five_prime_UTR	30.0%
	CAAATCACTGCAAAAGGAAA+AGG	-	tig0034656:28286-28305	Msa1453380:three_prime_UTR	35.0%
	TCCGGTATAGAATAGAAGTA+TGG	-	tig0034656:28347-28366	Msa1453380:exon	35.0%
!!	TTCTAATGCTGATTCTCCTT+GGG	+	tig0034656:28398-28417	None:intergenic	35.0%
!!	ATTCTAATGCTGATTCTCCT+TGG	+	tig0034656:28399-28418	None:intergenic	35.0%
	TTTGTGAAATTAGGGTTTCG+GGG	+	tig0034656:28432-28451	None:intergenic	35.0%
	GTGTTACCTGAAATTGAGAT+CGG	+	tig0034656:28503-28522	None:intergenic	35.0%
	CAACAAAGTTGTGATGTTGT+CGG	+	tig0034656:28686-28705	None:intergenic	35.0%
	AACTTTGTTGAAATCCGCAA+AGG	-	tig0034656:28696-28715	Msa1453380:intron	35.0%
	AGCAAGTTTAGCGAATAAAG+CGG	+	tig0034656:28743-28762	None:intergenic	35.0%
!	TTAGCTCTTTGAATACTGGT+TGG	-	tig0034656:28991-29010	Msa1453380:five_prime_UTR	35.0%
!	TTTGCTATCACTTGAGGATT+CGG	-	tig0034656:29031-29050	Msa1453380:five_prime_UTR	35.0%
!!	ATGATCCTAATGGATTGGTT+CGG	-	tig0034656:29336-29355	Msa1453380:five_prime_UTR	35.0%
	CAAATCACTGCAAAAGGAAA+AGG	-	tig0034656:28286-28305	Msa1453380:three_prime_UTR	35.0%
	TCCGGTATAGAATAGAAGTA+TGG	-	tig0034656:28347-28366	Msa1453380:exon	35.0%
!!	TTCTAATGCTGATTCTCCTT+GGG	+	tig0034656:28398-28417	None:intergenic	35.0%
!!	ATTCTAATGCTGATTCTCCT+TGG	+	tig0034656:28399-28418	None:intergenic	35.0%
	TTTGTGAAATTAGGGTTTCG+GGG	+	tig0034656:28432-28451	None:intergenic	35.0%
	GTGTTACCTGAAATTGAGAT+CGG	+	tig0034656:28503-28522	None:intergenic	35.0%
	CAACAAAGTTGTGATGTTGT+CGG	+	tig0034656:28686-28705	None:intergenic	35.0%
	AACTTTGTTGAAATCCGCAA+AGG	-	tig0034656:28696-28715	Msa1453380:intron	35.0%
	AGCAAGTTTAGCGAATAAAG+CGG	+	tig0034656:28743-28762	None:intergenic	35.0%
!	TTAGCTCTTTGAATACTGGT+TGG	-	tig0034656:28991-29010	Msa1453380:five_prime_UTR	35.0%
!	TTTGCTATCACTTGAGGATT+CGG	-	tig0034656:29031-29050	Msa1453380:five_prime_UTR	35.0%
!!	ATGATCCTAATGGATTGGTT+CGG	-	tig0034656:29336-29355	Msa1453380:five_prime_UTR	35.0%
!!	TCTCCTTGGGCTTTATGTTT+TGG	+	tig0034656:28385-28404	None:intergenic	40.0%
	TCAAGATCTGATCTGTTAGG+AGG	+	tig0034656:28575-28594	None:intergenic	40.0%
	TGTAGTGTGAAAATGTCGCT+CGG	-	tig0034656:28601-28620	Msa1453380:CDS	40.0%
	GGATTCAACATCAACCTTTG+CGG	+	tig0034656:28713-28732	None:intergenic	40.0%
!!	AGGTTGATGTTGAATCCTTC+TGG	-	tig0034656:28716-28735	Msa1453380:intron	40.0%
	GCAAGTTTAGCGAATAAAGC+GGG	+	tig0034656:28742-28761	None:intergenic	40.0%
!	ATTCGGCTTGTTCGATGAAT+AGG	-	tig0034656:28936-28955	Msa1453380:five_prime_UTR	40.0%
	ATGAACCGAACCAATCCATT+AGG	+	tig0034656:29344-29363	None:intergenic	40.0%
!!	TCTCCTTGGGCTTTATGTTT+TGG	+	tig0034656:28385-28404	None:intergenic	40.0%
	TCAAGATCTGATCTGTTAGG+AGG	+	tig0034656:28575-28594	None:intergenic	40.0%
	TGTAGTGTGAAAATGTCGCT+CGG	-	tig0034656:28601-28620	Msa1453380:CDS	40.0%
	GGATTCAACATCAACCTTTG+CGG	+	tig0034656:28713-28732	None:intergenic	40.0%
!!	AGGTTGATGTTGAATCCTTC+TGG	-	tig0034656:28716-28735	Msa1453380:intron	40.0%
	GCAAGTTTAGCGAATAAAGC+GGG	+	tig0034656:28742-28761	None:intergenic	40.0%
!	ATTCGGCTTGTTCGATGAAT+AGG	-	tig0034656:28936-28955	Msa1453380:five_prime_UTR	40.0%
	ATGAACCGAACCAATCCATT+AGG	+	tig0034656:29344-29363	None:intergenic	40.0%
	TCGTCTCCGATCTCAATTTC+AGG	-	tig0034656:28494-28513	Msa1453380:CDS	45.0%
	GTCATCTTCGAGGATGAGAA+GGG	+	tig0034656:28647-28666	None:intergenic	45.0%
	TGTCATCTTCGAGGATGAGA+AGG	+	tig0034656:28648-28667	None:intergenic	45.0%
	CTGGAATGTTGTCATCTTCG+AGG	+	tig0034656:28657-28676	None:intergenic	45.0%
	TGAGAAGAAACCAGAGCAAG+AGG	-	tig0034656:28888-28907	Msa1453380:five_prime_UTR	45.0%
	AGAAGAGAGTGACGAGGAAA+TGG	-	tig0034656:28912-28931	Msa1453380:five_prime_UTR	45.0%
	GAAGAGAGTGACGAGGAAAT+GGG	-	tig0034656:28913-28932	Msa1453380:five_prime_UTR	45.0%
	AGTGACGAGGAAATGGGATT+CGG	-	tig0034656:28919-28938	Msa1453380:five_prime_UTR	45.0%
	TCGTCTCCGATCTCAATTTC+AGG	-	tig0034656:28494-28513	Msa1453380:CDS	45.0%
	GTCATCTTCGAGGATGAGAA+GGG	+	tig0034656:28647-28666	None:intergenic	45.0%
	TGTCATCTTCGAGGATGAGA+AGG	+	tig0034656:28648-28667	None:intergenic	45.0%
	CTGGAATGTTGTCATCTTCG+AGG	+	tig0034656:28657-28676	None:intergenic	45.0%
	TGAGAAGAAACCAGAGCAAG+AGG	-	tig0034656:28888-28907	Msa1453380:five_prime_UTR	45.0%
	AGAAGAGAGTGACGAGGAAA+TGG	-	tig0034656:28912-28931	Msa1453380:five_prime_UTR	45.0%
	GAAGAGAGTGACGAGGAAAT+GGG	-	tig0034656:28913-28932	Msa1453380:five_prime_UTR	45.0%
	AGTGACGAGGAAATGGGATT+CGG	-	tig0034656:28919-28938	Msa1453380:five_prime_UTR	45.0%
	CACGTACACATGTGTGTGTG+TGG	+	tig0034656:28089-28108	None:intergenic	50.0%
!	GCTCAAGTCGAGTTTCGAGT+TGG	+	tig0034656:28193-28212	None:intergenic	50.0%
	GGTTACGAAGAGTAGCAGTC+CGG	-	tig0034656:28329-28348	Msa1453380:three_prime_UTR	50.0%
	GGGCCAAAACATAAAGCCCA+AGG	-	tig0034656:28379-28398	Msa1453380:CDS	50.0%
	AGACGACGAGAAGAGTAAGG+AGG	+	tig0034656:28480-28499	None:intergenic	50.0%
	GATTTGATTGCCAGTGCTGC+TGG	-	tig0034656:28781-28800	Msa1453380:intron	50.0%
	CTCTCTTCTTCCTCTTGCTC+TGG	+	tig0034656:28901-28920	None:intergenic	50.0%
	AGAGGAAGAAGAGAGTGACG+AGG	-	tig0034656:28906-28925	Msa1453380:five_prime_UTR	50.0%
	CACGTACACATGTGTGTGTG+TGG	+	tig0034656:28089-28108	None:intergenic	50.0%
!	GCTCAAGTCGAGTTTCGAGT+TGG	+	tig0034656:28193-28212	None:intergenic	50.0%
	GGTTACGAAGAGTAGCAGTC+CGG	-	tig0034656:28329-28348	Msa1453380:three_prime_UTR	50.0%
	GGGCCAAAACATAAAGCCCA+AGG	-	tig0034656:28379-28398	Msa1453380:CDS	50.0%
	AGACGACGAGAAGAGTAAGG+AGG	+	tig0034656:28480-28499	None:intergenic	50.0%
	GATTTGATTGCCAGTGCTGC+TGG	-	tig0034656:28781-28800	Msa1453380:intron	50.0%
	CTCTCTTCTTCCTCTTGCTC+TGG	+	tig0034656:28901-28920	None:intergenic	50.0%
	AGAGGAAGAAGAGAGTGACG+AGG	-	tig0034656:28906-28925	Msa1453380:five_prime_UTR	50.0%
	CGGAGACGACGAGAAGAGTA+AGG	+	tig0034656:28483-28502	None:intergenic	55.0%
	GTGATGTTGTCGGCAGTTGC+TGG	+	tig0034656:28676-28695	None:intergenic	55.0%
	AGCGAATAAAGCGGGCCAGA+AGG	+	tig0034656:28734-28753	None:intergenic	55.0%
!	TTGATTGCCAGTGCTGCTGG+AGG	-	tig0034656:28784-28803	Msa1453380:intron	55.0%
	TTCTTCTCAGCAGCAGCAGC+TGG	+	tig0034656:28877-28896	None:intergenic	55.0%
	CGGAGACGACGAGAAGAGTA+AGG	+	tig0034656:28483-28502	None:intergenic	55.0%
	GTGATGTTGTCGGCAGTTGC+TGG	+	tig0034656:28676-28695	None:intergenic	55.0%
	AGCGAATAAAGCGGGCCAGA+AGG	+	tig0034656:28734-28753	None:intergenic	55.0%
!	TTGATTGCCAGTGCTGCTGG+AGG	-	tig0034656:28784-28803	Msa1453380:intron	55.0%
	TTCTTCTCAGCAGCAGCAGC+TGG	+	tig0034656:28877-28896	None:intergenic	55.0%
!	ATTGCCAGTGCTGCTGGAGG+TGG	-	tig0034656:28787-28806	Msa1453380:intron	60.0%
	TGCTCCTGGAGCAGCTGTTG+CGG	-	tig0034656:28810-28829	Msa1453380:five_prime_UTR	60.0%
	GCTGCTCCAGTTGCAGCATC+AGG	-	tig0034656:28832-28851	Msa1453380:five_prime_UTR	60.0%
	GCTCCAGTTGCAGCATCAGG+TGG	-	tig0034656:28835-28854	Msa1453380:five_prime_UTR	60.0%
	CCACCACCTGATGCTGCAAC+TGG	+	tig0034656:28841-28860	None:intergenic	60.0%
	CCAGTTGCAGCATCAGGTGG+TGG	-	tig0034656:28838-28857	Msa1453380:five_prime_UTR	60.0%
!	ATTGCCAGTGCTGCTGGAGG+TGG	-	tig0034656:28787-28806	Msa1453380:intron	60.0%
	TGCTCCTGGAGCAGCTGTTG+CGG	-	tig0034656:28810-28829	Msa1453380:five_prime_UTR	60.0%
	GCTGCTCCAGTTGCAGCATC+AGG	-	tig0034656:28832-28851	Msa1453380:five_prime_UTR	60.0%
	GCTCCAGTTGCAGCATCAGG+TGG	-	tig0034656:28835-28854	Msa1453380:five_prime_UTR	60.0%
	CCACCACCTGATGCTGCAAC+TGG	+	tig0034656:28841-28860	None:intergenic	60.0%
	CCAGTTGCAGCATCAGGTGG+TGG	-	tig0034656:28838-28857	Msa1453380:five_prime_UTR	60.0%
	AGCACCACCTCCAGCAGCAC+TGG	+	tig0034656:28794-28813	None:intergenic	65.0%
	AGCAGCAGCTGGAGCAGCAG+CGG	+	tig0034656:28866-28885	None:intergenic	65.0%
	AGCACCACCTCCAGCAGCAC+TGG	+	tig0034656:28794-28813	None:intergenic	65.0%
	AGCAGCAGCTGGAGCAGCAG+CGG	+	tig0034656:28866-28885	None:intergenic	65.0%
	GCTGCTGGAGGTGGTGCTCC+TGG	-	tig0034656:28796-28815	Msa1453380:intron	70.0%
	GCAGCCGCAACAGCTGCTCC+AGG	+	tig0034656:28817-28836	None:intergenic	70.0%
	GCTGCTGGAGGTGGTGCTCC+TGG	-	tig0034656:28796-28815	Msa1453380:intron	70.0%
	GCAGCCGCAACAGCTGCTCC+AGG	+	tig0034656:28817-28836	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
tig0034656	gene	27932	29382	27932	ID=Msa1453380;Name=Msa1453380
tig0034656	mRNA	27932	29382	27932	ID=Msa1453380-mRNA-1;Parent=Msa1453380;Name=Msa1453380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=591\|1\|1\|1\|0\|0.5\|2\|425\|114
tig0034656	exon	27932	28708	27932	ID=Msa1453380-mRNA-1:exon:19118;Parent=Msa1453380-mRNA-1
tig0034656	exon	28799	29382	28799	ID=Msa1453380-mRNA-1:exon:19117;Parent=Msa1453380-mRNA-1
tig0034656	five_prime_UTR	28799	29382	28799	ID=Msa1453380-mRNA-1:five_prime_utr;Parent=Msa1453380-mRNA-1
tig0034656	five_prime_UTR	28702	28708	28702	ID=Msa1453380-mRNA-1:five_prime_utr;Parent=Msa1453380-mRNA-1
tig0034656	CDS	28357	28701	28357	ID=Msa1453380-mRNA-1:cds;Parent=Msa1453380-mRNA-1
tig0034656	three_prime_UTR	27932	28356	27932	ID=Msa1453380-mRNA-1:three_prime_utr;Parent=Msa1453380-mRNA-1

Gene Sequence	>Msa1453380 ATGTCGCTCGGAGAAACTGCTTGCTCATACGCCCTTCTCATCCTCGAAGATGACAACATTCCAGCAACTGCCGACAACATCACAACTTTGTTGAAATCCGCAAAGGTTGATGTTGAATCCTTCTGGCCCGCTTTATTCGCTAAACTTGCTGAGAAGAAAAATATTAGGGATTTGATTGCCAGTGCTGCTGGAGGTGGTGCTCCTGGAGCAGCTGTTGCGGCTGCTCCAGTTGCAGCATCAGGTGGTGGTGCCGCTGCTGCTCCAGCTGCTGCTGCTGAGAAGAAACCAGAGCAAGAGGAAGAAGAGAGTGACGAGGAAATGGGATTCGGCTTGTTCGATGAATAG
Protein sequence	>Msa1453380 MSLGETACSYALLILEDDNIPATADNITTLLKSAKVDVESFWPALFAKLAEKKNIRDLIASAAGGGAPGAAVAAAPVAASGGGAAAAPAAAAEKKPEQEEEESDEEMGFGLFDE*