AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0929680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0929680 MtrunA17_Chr8g0389431 100.000 121 0 0 1 121 1 121 4.21e-85 243
Msa0929680 MtrunA17_Chr6g0487951 97.521 121 3 0 1 121 1 121 6.56e-83 238
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0929680 AT5G56710.1 83.333 114 19 0 8 121 6 119 6.09e-67 197
Msa0929680 AT4G26230.1 82.456 114 20 0 8 121 6 119 2.15e-66 196
Msa0929680 AT2G19740.1 81.897 116 21 0 6 121 4 119 3.02e-66 196
Msa0929680 AT5G56710.2 58.772 114 13 1 8 121 6 85 6.55e-38 123

Find 76 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACTAAAATCTTATTAAAA+AGG 0.184411 6_2:-78491942 Msa0929680:three_prime_UTR
CTGATCTCCTTTATTGCTTT+AGG 0.186329 6_2:+78492420 None:intergenic
TCAAGTTGAACAAGGCTATT+TGG 0.242228 6_2:-78492350 Msa0929680:CDS
CATTAGTTCCCATAGCCTTT+TGG 0.252576 6_2:+78492388 None:intergenic
CAATCCTTTGAGCTCTTCCT+TGG 0.276372 6_2:+78492212 None:intergenic
GAAAGCTCCTAAAGCAATAA+AGG 0.311659 6_2:-78492427 Msa0929680:CDS
CTCATCATCGTTCCTCTTGC+GGG 0.315756 6_2:+78492281 None:intergenic
TATGAGGCCCATTAGACATT+AGG 0.319410 6_2:-78493328 Msa0929680:five_prime_UTR
TATAAAACCCTAATGTCTAA+TGG 0.323159 6_2:+78493320 None:intergenic
AATCCTTTGAGCTCTTCCTT+GGG 0.346185 6_2:+78492213 None:intergenic
ATATAGGTCACAAGTGAAAA+TGG 0.357698 6_2:-78493107 Msa0929680:intron
CAAATATACAAGTAGGCTTC+AGG 0.363096 6_2:-78491896 Msa0929680:three_prime_UTR
ATAAAACCCTAATGTCTAAT+GGG 0.373566 6_2:+78493321 None:intergenic
ATGAGGCCCATTAGACATTA+GGG 0.382081 6_2:-78493327 Msa0929680:five_prime_UTR
CTTGTATATTTGTTCAGAAC+AGG 0.386742 6_2:+78491907 None:intergenic
GAAGTGTCCCTAGGAGGGTT+AGG 0.392774 6_2:-78492314 Msa0929680:CDS
GAGGCGTTTGTGGAGGTTGA+TGG 0.411978 6_2:+78493033 None:intergenic
TACAGACGGCGGAACTCAAA+AGG 0.420451 6_2:+78493246 None:intergenic
TGTCTAATGGGCCTCATAGC+CGG 0.421249 6_2:+78493333 None:intergenic
GATCAGGAAGTTTGCCCAAA+AGG 0.422618 6_2:-78492403 Msa0929680:CDS
GGATGTAGCATTGTTGTGTA+TGG 0.423936 6_2:+78491821 None:intergenic
ATGCGAACCCTAACCCTCCT+AGG 0.432434 6_2:+78492306 None:intergenic
CTAAAGCAATAAAGGAGATC+AGG 0.445899 6_2:-78492419 Msa0929680:CDS
AAGGAAGAGCTCAAAGGATT+GGG 0.447041 6_2:-78492210 Msa0929680:CDS
ACCAGCCATGGAGGCGTTTG+TGG 0.449631 6_2:+78493023 None:intergenic
ATGTTTAATCTTTCTAAGAC+AGG 0.451506 6_2:+78492045 None:intergenic
CAAGGAAGAGCTCAAAGGAT+TGG 0.452186 6_2:-78492211 Msa0929680:CDS
CAAGAAAACAAAACAGAGAA+AGG 0.452389 6_2:+78492083 None:intergenic
CCTCATCATCGTTCCTCTTG+CGG 0.459607 6_2:+78492280 None:intergenic
GAACAAGGCTATTTGGAGCC+AGG 0.460263 6_2:-78492343 Msa0929680:CDS
TATATTTAATTCTGACATTG+AGG 0.473217 6_2:-78492131 Msa0929680:three_prime_UTR
TCCACAAACGCCTCCATGGC+TGG 0.473615 6_2:-78493024 Msa0929680:intron
TAGGGACACTTCTGATCCCC+TGG 0.478265 6_2:+78492325 None:intergenic
GTCTAATGGGCCTCATAGCC+GGG 0.481758 6_2:+78493334 None:intergenic
CTCTTCCTTCCTACCGGCGC+CGG 0.482937 6_2:+78493075 None:intergenic
GAAGTTTGCCCAAAAGGCTA+TGG 0.483900 6_2:-78492397 Msa0929680:CDS
GTGAAAATGGTGGAGAAGAC+CGG 0.499433 6_2:-78493094 Msa0929680:exon
GAGGTTGATGGTGTACTCTC+TGG 0.499941 6_2:+78493045 None:intergenic
AAGTGTCCCTAGGAGGGTTA+GGG 0.512529 6_2:-78492313 Msa0929680:CDS
ATACACAACAATGCTACATC+CGG 0.514282 6_2:-78491819 Msa0929680:three_prime_UTR
ATCCTTTGAGCTCTTCCTTG+GGG 0.520497 6_2:+78492214 None:intergenic
AACAAGGCTATTTGGAGCCA+GGG 0.528204 6_2:-78492342 Msa0929680:CDS
AGAGAGAGAATTACCAGCCA+TGG 0.536558 6_2:+78493011 None:intergenic
CACAAAGGTTATTGATGATG+AGG 0.542190 6_2:-78492187 Msa0929680:CDS
GAACGATGATGAGGATGCTA+AGG 0.543980 6_2:-78492271 Msa0929680:CDS
GAAGACCGGCGCCGGTAGGA+AGG 0.546764 6_2:-78493080 Msa0929680:CDS
CGGCGCCGGTAGGAAGGAAG+AGG 0.547239 6_2:-78493074 Msa0929680:CDS
ATTCAGCAAGTAGATTATCA+AGG 0.553229 6_2:+78491796 None:intergenic
ACAGACGGCGGAACTCAAAA+GGG 0.553650 6_2:+78493247 None:intergenic
AGCAAGTAGATTATCAAGGC+CGG 0.553903 6_2:+78491800 None:intergenic
TTGAGTGGAACCCGGCTATG+AGG 0.559647 6_2:-78493344 None:intergenic
AACCTCCACAAACGCCTCCA+TGG 0.560698 6_2:-78493028 Msa0929680:CDS
AGGGGATCAGAAGTGTCCCT+AGG 0.578761 6_2:-78492323 Msa0929680:CDS
GACAACCTCTTCCTTCCTAC+CGG 0.582542 6_2:+78493069 None:intergenic
AAGTTTGCCCAAAAGGCTAT+GGG 0.584767 6_2:-78492396 Msa0929680:CDS
AGCCATGGAGGCGTTTGTGG+AGG 0.589507 6_2:+78493026 None:intergenic
TACTGTTGTTGAAATCCCCA+AGG 0.603575 6_2:-78492229 Msa0929680:CDS
CCGCAAGAGGAACGATGATG+AGG 0.607958 6_2:-78492280 Msa0929680:CDS
TGGAGAAGACCGGCGCCGGT+AGG 0.608791 6_2:-78493084 Msa0929680:CDS
GATCAGAAGTGTCCCTAGGA+GGG 0.609828 6_2:-78492319 Msa0929680:CDS
ATGGTGGAGAAGACCGGCGC+CGG 0.610511 6_2:-78493088 Msa0929680:CDS
TGCGAACCCTAACCCTCCTA+GGG 0.611019 6_2:+78492307 None:intergenic
GGGTTCGCATTGCCCGCAAG+AGG 0.617342 6_2:-78492293 Msa0929680:CDS
GGATCAGAAGTGTCCCTAGG+AGG 0.618801 6_2:-78492320 Msa0929680:CDS
TTCTGAACAAATATACAAGT+AGG 0.628910 6_2:-78491903 Msa0929680:three_prime_UTR
GGAGAGCAAACGAACGAACG+AGG 0.633628 6_2:+78493268 None:intergenic
ATCCCCAAGGAAGAGCTCAA+AGG 0.635127 6_2:-78492216 Msa0929680:CDS
AAGCGCAGAAAAGAAAAGAG+AGG 0.645316 6_2:+78492008 None:intergenic
GAGAGAATTACCAGCCATGG+AGG 0.648989 6_2:+78493014 None:intergenic
ACAAGGCTATTTGGAGCCAG+GGG 0.649532 6_2:-78492341 Msa0929680:CDS
GCTCAAAGGATTGGGCACAA+AGG 0.653987 6_2:-78492202 Msa0929680:CDS
TAGGTCACAAGTGAAAATGG+TGG 0.656235 6_2:-78493104 Msa0929680:exon
TACGATCTGGAATATACAGA+CGG 0.660204 6_2:+78493232 None:intergenic
AGTGGACGTCAAGTTGAACA+AGG 0.683327 6_2:-78492358 Msa0929680:CDS
GGGAACTAATGATGTCAGAG+TGG 0.705766 6_2:-78492376 Msa0929680:CDS
GATCTGGAATATACAGACGG+CGG 0.791885 6_2:+78493235 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATGTATTTGTTTATTATAT+AGG - chr6_2:78492016-78492035 Msa0929680:three_prime_UTR 15.0%
!!! ATTTTTTTAATCTCTTTAGA+AGG - chr6_2:78492258-78492277 Msa0929680:CDS 15.0%
!!! TTTACAAATATTTTGATAAC+CGG - chr6_2:78492503-78492522 Msa0929680:intron 15.0%
!! TCAGAATTAAATATAAAAAC+CGG + chr6_2:78493003-78493022 None:intergenic 15.0%
!! AAAATCTTATTAAAAAGGTT+TGG - chr6_2:78493202-78493221 Msa0929680:intron 15.0%
!! CATGTATTTGTTTATTATAT+AGG - chr6_2:78492016-78492035 Msa0929680:three_prime_UTR 15.0%
!!! ATTTTTTTAATCTCTTTAGA+AGG - chr6_2:78492258-78492277 Msa0929680:CDS 15.0%
!!! TTTACAAATATTTTGATAAC+CGG - chr6_2:78492503-78492522 Msa0929680:intron 15.0%
!! TCAGAATTAAATATAAAAAC+CGG + chr6_2:78493003-78493022 None:intergenic 15.0%
!! AAAATCTTATTAAAAAGGTT+TGG - chr6_2:78493202-78493221 Msa0929680:intron 15.0%
!! ATCATAAATTGTTTGTGATT+CGG + chr6_2:78491981-78492000 None:intergenic 20.0%
!! TCACAAACAATTTATGATAA+TGG - chr6_2:78491983-78492002 Msa0929680:three_prime_UTR 20.0%
!! ATCAGTTAAAATAACATCTA+CGG + chr6_2:78492484-78492503 None:intergenic 20.0%
!!! TTACAAATATTTTGATAACC+GGG - chr6_2:78492504-78492523 Msa0929680:intron 20.0%
!! TATATTTAATTCTGACATTG+AGG - chr6_2:78493008-78493027 Msa0929680:intron 20.0%
!! CGACTAAAATCTTATTAAAA+AGG - chr6_2:78493197-78493216 Msa0929680:intron 20.0%
!! ATCATAAATTGTTTGTGATT+CGG + chr6_2:78491981-78492000 None:intergenic 20.0%
!! TCACAAACAATTTATGATAA+TGG - chr6_2:78491983-78492002 Msa0929680:three_prime_UTR 20.0%
!! ATCAGTTAAAATAACATCTA+CGG + chr6_2:78492484-78492503 None:intergenic 20.0%
!!! TTACAAATATTTTGATAACC+GGG - chr6_2:78492504-78492523 Msa0929680:intron 20.0%
!! TATATTTAATTCTGACATTG+AGG - chr6_2:78493008-78493027 Msa0929680:intron 20.0%
!! CGACTAAAATCTTATTAAAA+AGG - chr6_2:78493197-78493216 Msa0929680:intron 20.0%
! ATAAAACCCTAATGTCTAAT+GGG + chr6_2:78491821-78491840 None:intergenic 25.0%
! TATAAAACCCTAATGTCTAA+TGG + chr6_2:78491822-78491841 None:intergenic 25.0%
! TCATAAATTGTTTGTGATTC+GGG + chr6_2:78491980-78491999 None:intergenic 25.0%
!! AACAAAACATTTGCTAGATA+GGG + chr6_2:78492187-78492206 None:intergenic 25.0%
!! AAACAAAACATTTGCTAGAT+AGG + chr6_2:78492188-78492207 None:intergenic 25.0%
!! GATATAGAGGAAAATTTTTG+TGG - chr6_2:78492219-78492238 Msa0929680:CDS 25.0%
!!! TCTTTAGAAGGTTTTTTCAA+TGG - chr6_2:78492270-78492289 Msa0929680:CDS 25.0%
! TAAATAGCATATCACAGAAA+TGG - chr6_2:78492427-78492446 Msa0929680:CDS 25.0%
!!! ATGTTTAATCTTTCTAAGAC+AGG + chr6_2:78493097-78493116 None:intergenic 25.0%
! TTCTGAACAAATATACAAGT+AGG - chr6_2:78493236-78493255 Msa0929680:five_prime_UTR 25.0%
! ATAAAACCCTAATGTCTAAT+GGG + chr6_2:78491821-78491840 None:intergenic 25.0%
! TATAAAACCCTAATGTCTAA+TGG + chr6_2:78491822-78491841 None:intergenic 25.0%
! TCATAAATTGTTTGTGATTC+GGG + chr6_2:78491980-78491999 None:intergenic 25.0%
!! AACAAAACATTTGCTAGATA+GGG + chr6_2:78492187-78492206 None:intergenic 25.0%
!! AAACAAAACATTTGCTAGAT+AGG + chr6_2:78492188-78492207 None:intergenic 25.0%
!! GATATAGAGGAAAATTTTTG+TGG - chr6_2:78492219-78492238 Msa0929680:CDS 25.0%
!!! TCTTTAGAAGGTTTTTTCAA+TGG - chr6_2:78492270-78492289 Msa0929680:CDS 25.0%
! TAAATAGCATATCACAGAAA+TGG - chr6_2:78492427-78492446 Msa0929680:CDS 25.0%
!!! ATGTTTAATCTTTCTAAGAC+AGG + chr6_2:78493097-78493116 None:intergenic 25.0%
! TTCTGAACAAATATACAAGT+AGG - chr6_2:78493236-78493255 Msa0929680:five_prime_UTR 25.0%
ATATAGGTCACAAGTGAAAA+TGG - chr6_2:78492032-78492051 Msa0929680:three_prime_UTR 30.0%
! TTGTTTTCGATCAGATATAG+AGG - chr6_2:78492206-78492225 Msa0929680:CDS 30.0%
! CATCAAAATGAAAAGCACAA+GGG + chr6_2:78492308-78492327 None:intergenic 30.0%
! TCATCAAAATGAAAAGCACA+AGG + chr6_2:78492309-78492328 None:intergenic 30.0%
! AAAAGTCATTTCAGGTAAAG+GGG + chr6_2:78492578-78492597 None:intergenic 30.0%
! GAAAAGTCATTTCAGGTAAA+GGG + chr6_2:78492579-78492598 None:intergenic 30.0%
! AGAAAAGTCATTTCAGGTAA+AGG + chr6_2:78492580-78492599 None:intergenic 30.0%
! TACCTGAAATGACTTTTCTA+TGG - chr6_2:78492581-78492600 Msa0929680:intron 30.0%
! CTTTTCTATGGAGATTGTTT+CGG - chr6_2:78492593-78492612 Msa0929680:intron 30.0%
!! GCTGTTTTGCATAATAACAA+AGG + chr6_2:78492681-78492700 None:intergenic 30.0%
!!! CTCTTTTAGCTTTGTTAATC+CGG - chr6_2:78492981-78493000 Msa0929680:intron 30.0%
CAAGAAAACAAAACAGAGAA+AGG + chr6_2:78493059-78493078 None:intergenic 30.0%
CTTGTATATTTGTTCAGAAC+AGG + chr6_2:78493235-78493254 None:intergenic 30.0%
ATATAGGTCACAAGTGAAAA+TGG - chr6_2:78492032-78492051 Msa0929680:three_prime_UTR 30.0%
! TTGTTTTCGATCAGATATAG+AGG - chr6_2:78492206-78492225 Msa0929680:CDS 30.0%
! CATCAAAATGAAAAGCACAA+GGG + chr6_2:78492308-78492327 None:intergenic 30.0%
! TCATCAAAATGAAAAGCACA+AGG + chr6_2:78492309-78492328 None:intergenic 30.0%
! AAAAGTCATTTCAGGTAAAG+GGG + chr6_2:78492578-78492597 None:intergenic 30.0%
! GAAAAGTCATTTCAGGTAAA+GGG + chr6_2:78492579-78492598 None:intergenic 30.0%
! AGAAAAGTCATTTCAGGTAA+AGG + chr6_2:78492580-78492599 None:intergenic 30.0%
! TACCTGAAATGACTTTTCTA+TGG - chr6_2:78492581-78492600 Msa0929680:intron 30.0%
! CTTTTCTATGGAGATTGTTT+CGG - chr6_2:78492593-78492612 Msa0929680:intron 30.0%
!! GCTGTTTTGCATAATAACAA+AGG + chr6_2:78492681-78492700 None:intergenic 30.0%
!!! CTCTTTTAGCTTTGTTAATC+CGG - chr6_2:78492981-78493000 Msa0929680:intron 30.0%
CAAGAAAACAAAACAGAGAA+AGG + chr6_2:78493059-78493078 None:intergenic 30.0%
CTTGTATATTTGTTCAGAAC+AGG + chr6_2:78493235-78493254 None:intergenic 30.0%
! TACGATCTGGAATATACAGA+CGG + chr6_2:78491910-78491929 None:intergenic 35.0%
GGATAAACAAAGTGGAAAGA+TGG + chr6_2:78491959-78491978 None:intergenic 35.0%
ATAGCATATCACAGAAATGG+TGG - chr6_2:78492430-78492449 Msa0929680:CDS 35.0%
!!! CAAATATTTTGATAACCGGG+AGG - chr6_2:78492507-78492526 Msa0929680:intron 35.0%
CTCCATAGAAAAGTCATTTC+AGG + chr6_2:78492586-78492605 None:intergenic 35.0%
GAAAGCTCCTAAAGCAATAA+AGG - chr6_2:78492712-78492731 Msa0929680:intron 35.0%
CTGATCTCCTTTATTGCTTT+AGG + chr6_2:78492722-78492741 None:intergenic 35.0%
CTAAAGCAATAAAGGAGATC+AGG - chr6_2:78492720-78492739 Msa0929680:intron 35.0%
! ATTAGTTCCCATAGCCTTTT+GGG + chr6_2:78492753-78492772 None:intergenic 35.0%
!! TCAAGTTGAACAAGGCTATT+TGG - chr6_2:78492789-78492808 Msa0929680:intron 35.0%
!! CACAAAGGTTATTGATGATG+AGG - chr6_2:78492952-78492971 Msa0929680:intron 35.0%
CAAATATACAAGTAGGCTTC+AGG - chr6_2:78493243-78493262 Msa0929680:five_prime_UTR 35.0%
ATACACAACAATGCTACATC+CGG - chr6_2:78493320-78493339 Msa0929680:five_prime_UTR 35.0%
! TACGATCTGGAATATACAGA+CGG + chr6_2:78491910-78491929 None:intergenic 35.0%
GGATAAACAAAGTGGAAAGA+TGG + chr6_2:78491959-78491978 None:intergenic 35.0%
ATAGCATATCACAGAAATGG+TGG - chr6_2:78492430-78492449 Msa0929680:CDS 35.0%
!!! CAAATATTTTGATAACCGGG+AGG - chr6_2:78492507-78492526 Msa0929680:intron 35.0%
CTCCATAGAAAAGTCATTTC+AGG + chr6_2:78492586-78492605 None:intergenic 35.0%
GAAAGCTCCTAAAGCAATAA+AGG - chr6_2:78492712-78492731 Msa0929680:intron 35.0%
CTGATCTCCTTTATTGCTTT+AGG + chr6_2:78492722-78492741 None:intergenic 35.0%
CTAAAGCAATAAAGGAGATC+AGG - chr6_2:78492720-78492739 Msa0929680:intron 35.0%
! ATTAGTTCCCATAGCCTTTT+GGG + chr6_2:78492753-78492772 None:intergenic 35.0%
!! TCAAGTTGAACAAGGCTATT+TGG - chr6_2:78492789-78492808 Msa0929680:intron 35.0%
!! CACAAAGGTTATTGATGATG+AGG - chr6_2:78492952-78492971 Msa0929680:intron 35.0%
CAAATATACAAGTAGGCTTC+AGG - chr6_2:78493243-78493262 Msa0929680:five_prime_UTR 35.0%
ATACACAACAATGCTACATC+CGG - chr6_2:78493320-78493339 Msa0929680:five_prime_UTR 35.0%
TATGAGGCCCATTAGACATT+AGG - chr6_2:78491811-78491830 Msa0929680:three_prime_UTR 40.0%
ATGAGGCCCATTAGACATTA+GGG - chr6_2:78491812-78491831 Msa0929680:three_prime_UTR 40.0%
GTTAAAGAAGCAGAAGTGGA+CGG + chr6_2:78491937-78491956 None:intergenic 40.0%
GATGGTTAAAGAAGCAGAAG+TGG + chr6_2:78491941-78491960 None:intergenic 40.0%
! GTGATTCGGGATAAACAAAG+TGG + chr6_2:78491967-78491986 None:intergenic 40.0%
TAGGTCACAAGTGAAAATGG+TGG - chr6_2:78492035-78492054 Msa0929680:three_prime_UTR 40.0%
!!! ATATTTTGATAACCGGGAGG+AGG - chr6_2:78492510-78492529 Msa0929680:intron 40.0%
CAAAACTATATTCCTCCTCC+CGG + chr6_2:78492525-78492544 None:intergenic 40.0%
AAGTTTGCCCAAAAGGCTAT+GGG - chr6_2:78492743-78492762 Msa0929680:intron 40.0%
! CATTAGTTCCCATAGCCTTT+TGG + chr6_2:78492754-78492773 None:intergenic 40.0%
TACTGTTGTTGAAATCCCCA+AGG - chr6_2:78492910-78492929 Msa0929680:intron 40.0%
AATCCTTTGAGCTCTTCCTT+GGG + chr6_2:78492929-78492948 None:intergenic 40.0%
AAGGAAGAGCTCAAAGGATT+GGG - chr6_2:78492929-78492948 Msa0929680:intron 40.0%
AAGCGCAGAAAAGAAAAGAG+AGG + chr6_2:78493134-78493153 None:intergenic 40.0%
! TCTCTTTTCTTTTCTGCGCT+TGG - chr6_2:78493133-78493152 Msa0929680:intron 40.0%
GGATGTAGCATTGTTGTGTA+TGG + chr6_2:78493321-78493340 None:intergenic 40.0%
TATGAGGCCCATTAGACATT+AGG - chr6_2:78491811-78491830 Msa0929680:three_prime_UTR 40.0%
ATGAGGCCCATTAGACATTA+GGG - chr6_2:78491812-78491831 Msa0929680:three_prime_UTR 40.0%
GTTAAAGAAGCAGAAGTGGA+CGG + chr6_2:78491937-78491956 None:intergenic 40.0%
GATGGTTAAAGAAGCAGAAG+TGG + chr6_2:78491941-78491960 None:intergenic 40.0%
! GTGATTCGGGATAAACAAAG+TGG + chr6_2:78491967-78491986 None:intergenic 40.0%
TAGGTCACAAGTGAAAATGG+TGG - chr6_2:78492035-78492054 Msa0929680:three_prime_UTR 40.0%
!!! ATATTTTGATAACCGGGAGG+AGG - chr6_2:78492510-78492529 Msa0929680:intron 40.0%
CAAAACTATATTCCTCCTCC+CGG + chr6_2:78492525-78492544 None:intergenic 40.0%
AAGTTTGCCCAAAAGGCTAT+GGG - chr6_2:78492743-78492762 Msa0929680:intron 40.0%
! CATTAGTTCCCATAGCCTTT+TGG + chr6_2:78492754-78492773 None:intergenic 40.0%
TACTGTTGTTGAAATCCCCA+AGG - chr6_2:78492910-78492929 Msa0929680:intron 40.0%
AATCCTTTGAGCTCTTCCTT+GGG + chr6_2:78492929-78492948 None:intergenic 40.0%
AAGGAAGAGCTCAAAGGATT+GGG - chr6_2:78492929-78492948 Msa0929680:intron 40.0%
AAGCGCAGAAAAGAAAAGAG+AGG + chr6_2:78493134-78493153 None:intergenic 40.0%
! TCTCTTTTCTTTTCTGCGCT+TGG - chr6_2:78493133-78493152 Msa0929680:intron 40.0%
GGATGTAGCATTGTTGTGTA+TGG + chr6_2:78493321-78493340 None:intergenic 40.0%
! GATCTGGAATATACAGACGG+CGG + chr6_2:78491907-78491926 None:intergenic 45.0%
! GTGAAAATGGTGGAGAAGAC+CGG - chr6_2:78492045-78492064 Msa0929680:three_prime_UTR 45.0%
AGAGAGAGAATTACCAGCCA+TGG + chr6_2:78492131-78492150 None:intergenic 45.0%
GATCAGGAAGTTTGCCCAAA+AGG - chr6_2:78492736-78492755 Msa0929680:intron 45.0%
GAAGTTTGCCCAAAAGGCTA+TGG - chr6_2:78492742-78492761 Msa0929680:intron 45.0%
GGGAACTAATGATGTCAGAG+TGG - chr6_2:78492763-78492782 Msa0929680:intron 45.0%
!! AGTGGACGTCAAGTTGAACA+AGG - chr6_2:78492781-78492800 Msa0929680:intron 45.0%
! AACAAGGCTATTTGGAGCCA+GGG - chr6_2:78492797-78492816 Msa0929680:intron 45.0%
GAACGATGATGAGGATGCTA+AGG - chr6_2:78492868-78492887 Msa0929680:intron 45.0%
ATCCTTTGAGCTCTTCCTTG+GGG + chr6_2:78492928-78492947 None:intergenic 45.0%
CAATCCTTTGAGCTCTTCCT+TGG + chr6_2:78492930-78492949 None:intergenic 45.0%
CAAGGAAGAGCTCAAAGGAT+TGG - chr6_2:78492928-78492947 Msa0929680:intron 45.0%
! ACATCCGGCCTTGATAATCT+CGG - chr6_2:78493335-78493354 Msa0929680:five_prime_UTR 45.0%
! GATCTGGAATATACAGACGG+CGG + chr6_2:78491907-78491926 None:intergenic 45.0%
! GTGAAAATGGTGGAGAAGAC+CGG - chr6_2:78492045-78492064 Msa0929680:three_prime_UTR 45.0%
AGAGAGAGAATTACCAGCCA+TGG + chr6_2:78492131-78492150 None:intergenic 45.0%
GATCAGGAAGTTTGCCCAAA+AGG - chr6_2:78492736-78492755 Msa0929680:intron 45.0%
GAAGTTTGCCCAAAAGGCTA+TGG - chr6_2:78492742-78492761 Msa0929680:intron 45.0%
GGGAACTAATGATGTCAGAG+TGG - chr6_2:78492763-78492782 Msa0929680:intron 45.0%
!! AGTGGACGTCAAGTTGAACA+AGG - chr6_2:78492781-78492800 Msa0929680:intron 45.0%
! AACAAGGCTATTTGGAGCCA+GGG - chr6_2:78492797-78492816 Msa0929680:intron 45.0%
GAACGATGATGAGGATGCTA+AGG - chr6_2:78492868-78492887 Msa0929680:intron 45.0%
ATCCTTTGAGCTCTTCCTTG+GGG + chr6_2:78492928-78492947 None:intergenic 45.0%
CAATCCTTTGAGCTCTTCCT+TGG + chr6_2:78492930-78492949 None:intergenic 45.0%
CAAGGAAGAGCTCAAAGGAT+TGG - chr6_2:78492928-78492947 Msa0929680:intron 45.0%
ACAGACGGCGGAACTCAAAA+GGG + chr6_2:78491895-78491914 None:intergenic 50.0%
TACAGACGGCGGAACTCAAA+AGG + chr6_2:78491896-78491915 None:intergenic 50.0%
AGTGGACGGAACTTACGATC+TGG + chr6_2:78491923-78491942 None:intergenic 50.0%
GACAACCTCTTCCTTCCTAC+CGG + chr6_2:78492073-78492092 None:intergenic 50.0%
!! GAGGTTGATGGTGTACTCTC+TGG + chr6_2:78492097-78492116 None:intergenic 50.0%
GAGAGAATTACCAGCCATGG+AGG + chr6_2:78492128-78492147 None:intergenic 50.0%
! GAACAAGGCTATTTGGAGCC+AGG - chr6_2:78492796-78492815 Msa0929680:intron 50.0%
! ACAAGGCTATTTGGAGCCAG+GGG - chr6_2:78492798-78492817 Msa0929680:intron 50.0%
!! GATCAGAAGTGTCCCTAGGA+GGG - chr6_2:78492820-78492839 Msa0929680:intron 50.0%
AAGTGTCCCTAGGAGGGTTA+GGG - chr6_2:78492826-78492845 Msa0929680:intron 50.0%
CTCATCATCGTTCCTCTTGC+GGG + chr6_2:78492861-78492880 None:intergenic 50.0%
CCTCATCATCGTTCCTCTTG+CGG + chr6_2:78492862-78492881 None:intergenic 50.0%
ATCCCCAAGGAAGAGCTCAA+AGG - chr6_2:78492923-78492942 Msa0929680:intron 50.0%
GCTCAAAGGATTGGGCACAA+AGG - chr6_2:78492937-78492956 Msa0929680:intron 50.0%
ACAGACGGCGGAACTCAAAA+GGG + chr6_2:78491895-78491914 None:intergenic 50.0%
TACAGACGGCGGAACTCAAA+AGG + chr6_2:78491896-78491915 None:intergenic 50.0%
AGTGGACGGAACTTACGATC+TGG + chr6_2:78491923-78491942 None:intergenic 50.0%
GACAACCTCTTCCTTCCTAC+CGG + chr6_2:78492073-78492092 None:intergenic 50.0%
!! GAGGTTGATGGTGTACTCTC+TGG + chr6_2:78492097-78492116 None:intergenic 50.0%
GAGAGAATTACCAGCCATGG+AGG + chr6_2:78492128-78492147 None:intergenic 50.0%
! GAACAAGGCTATTTGGAGCC+AGG - chr6_2:78492796-78492815 Msa0929680:intron 50.0%
! ACAAGGCTATTTGGAGCCAG+GGG - chr6_2:78492798-78492817 Msa0929680:intron 50.0%
!! GATCAGAAGTGTCCCTAGGA+GGG - chr6_2:78492820-78492839 Msa0929680:intron 50.0%
AAGTGTCCCTAGGAGGGTTA+GGG - chr6_2:78492826-78492845 Msa0929680:intron 50.0%
CTCATCATCGTTCCTCTTGC+GGG + chr6_2:78492861-78492880 None:intergenic 50.0%
CCTCATCATCGTTCCTCTTG+CGG + chr6_2:78492862-78492881 None:intergenic 50.0%
ATCCCCAAGGAAGAGCTCAA+AGG - chr6_2:78492923-78492942 Msa0929680:intron 50.0%
GCTCAAAGGATTGGGCACAA+AGG - chr6_2:78492937-78492956 Msa0929680:intron 50.0%
GGAGAGCAAACGAACGAACG+AGG + chr6_2:78491874-78491893 None:intergenic 55.0%
! GAGGCGTTTGTGGAGGTTGA+TGG + chr6_2:78492109-78492128 None:intergenic 55.0%
AACCTCCACAAACGCCTCCA+TGG - chr6_2:78492111-78492130 Msa0929680:three_prime_UTR 55.0%
TAGGGACACTTCTGATCCCC+TGG + chr6_2:78492817-78492836 None:intergenic 55.0%
!! AGGGGATCAGAAGTGTCCCT+AGG - chr6_2:78492816-78492835 Msa0929680:intron 55.0%
!! GGATCAGAAGTGTCCCTAGG+AGG - chr6_2:78492819-78492838 Msa0929680:intron 55.0%
! GAAGTGTCCCTAGGAGGGTT+AGG - chr6_2:78492825-78492844 Msa0929680:intron 55.0%
TGCGAACCCTAACCCTCCTA+GGG + chr6_2:78492835-78492854 None:intergenic 55.0%
ATGCGAACCCTAACCCTCCT+AGG + chr6_2:78492836-78492855 None:intergenic 55.0%
CCGCAAGAGGAACGATGATG+AGG - chr6_2:78492859-78492878 Msa0929680:intron 55.0%
GGAGAGCAAACGAACGAACG+AGG + chr6_2:78491874-78491893 None:intergenic 55.0%
! GAGGCGTTTGTGGAGGTTGA+TGG + chr6_2:78492109-78492128 None:intergenic 55.0%
AACCTCCACAAACGCCTCCA+TGG - chr6_2:78492111-78492130 Msa0929680:three_prime_UTR 55.0%
TAGGGACACTTCTGATCCCC+TGG + chr6_2:78492817-78492836 None:intergenic 55.0%
!! AGGGGATCAGAAGTGTCCCT+AGG - chr6_2:78492816-78492835 Msa0929680:intron 55.0%
!! GGATCAGAAGTGTCCCTAGG+AGG - chr6_2:78492819-78492838 Msa0929680:intron 55.0%
! GAAGTGTCCCTAGGAGGGTT+AGG - chr6_2:78492825-78492844 Msa0929680:intron 55.0%
TGCGAACCCTAACCCTCCTA+GGG + chr6_2:78492835-78492854 None:intergenic 55.0%
ATGCGAACCCTAACCCTCCT+AGG + chr6_2:78492836-78492855 None:intergenic 55.0%
CCGCAAGAGGAACGATGATG+AGG - chr6_2:78492859-78492878 Msa0929680:intron 55.0%
!! AGCCATGGAGGCGTTTGTGG+AGG + chr6_2:78492116-78492135 None:intergenic 60.0%
TCCACAAACGCCTCCATGGC+TGG - chr6_2:78492115-78492134 Msa0929680:three_prime_UTR 60.0%
!! ACCAGCCATGGAGGCGTTTG+TGG + chr6_2:78492119-78492138 None:intergenic 60.0%
!! AGCCATGGAGGCGTTTGTGG+AGG + chr6_2:78492116-78492135 None:intergenic 60.0%
TCCACAAACGCCTCCATGGC+TGG - chr6_2:78492115-78492134 Msa0929680:three_prime_UTR 60.0%
!! ACCAGCCATGGAGGCGTTTG+TGG + chr6_2:78492119-78492138 None:intergenic 60.0%
! ATGGTGGAGAAGACCGGCGC+CGG - chr6_2:78492051-78492070 Msa0929680:three_prime_UTR 65.0%
CTCTTCCTTCCTACCGGCGC+CGG + chr6_2:78492067-78492086 None:intergenic 65.0%
GGGTTCGCATTGCCCGCAAG+AGG - chr6_2:78492846-78492865 Msa0929680:intron 65.0%
! ATGGTGGAGAAGACCGGCGC+CGG - chr6_2:78492051-78492070 Msa0929680:three_prime_UTR 65.0%
CTCTTCCTTCCTACCGGCGC+CGG + chr6_2:78492067-78492086 None:intergenic 65.0%
GGGTTCGCATTGCCCGCAAG+AGG - chr6_2:78492846-78492865 Msa0929680:intron 65.0%
!! TGGAGAAGACCGGCGCCGGT+AGG - chr6_2:78492055-78492074 Msa0929680:three_prime_UTR 70.0%
!! GAAGACCGGCGCCGGTAGGA+AGG - chr6_2:78492059-78492078 Msa0929680:three_prime_UTR 70.0%
!! CGGCGCCGGTAGGAAGGAAG+AGG - chr6_2:78492065-78492084 Msa0929680:three_prime_UTR 70.0%
!! TGGAGAAGACCGGCGCCGGT+AGG - chr6_2:78492055-78492074 Msa0929680:three_prime_UTR 70.0%
!! GAAGACCGGCGCCGGTAGGA+AGG - chr6_2:78492059-78492078 Msa0929680:three_prime_UTR 70.0%
!! CGGCGCCGGTAGGAAGGAAG+AGG - chr6_2:78492065-78492084 Msa0929680:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 78491807 78493354 78491807 ID=Msa0929680;Name=Msa0929680
chr6_2 mRNA 78491807 78493354 78491807 ID=Msa0929680-mRNA-1;Parent=Msa0929680;Name=Msa0929680-mRNA-1;_AED=0.02;_eAED=0.02;_QI=133|1|1|1|0.5|0.33|3|375|121
chr6_2 exon 78491807 78492461 78491807 ID=Msa0929680-mRNA-1:exon:13190;Parent=Msa0929680-mRNA-1
chr6_2 exon 78493025 78493123 78493025 ID=Msa0929680-mRNA-1:exon:13189;Parent=Msa0929680-mRNA-1
chr6_2 exon 78493235 78493354 78493235 ID=Msa0929680-mRNA-1:exon:13188;Parent=Msa0929680-mRNA-1
chr6_2 five_prime_UTR 78493235 78493354 78493235 ID=Msa0929680-mRNA-1:five_prime_utr;Parent=Msa0929680-mRNA-1
chr6_2 five_prime_UTR 78493111 78493123 78493111 ID=Msa0929680-mRNA-1:five_prime_utr;Parent=Msa0929680-mRNA-1
chr6_2 CDS 78493025 78493110 78493025 ID=Msa0929680-mRNA-1:cds;Parent=Msa0929680-mRNA-1
chr6_2 CDS 78492182 78492461 78492182 ID=Msa0929680-mRNA-1:cds;Parent=Msa0929680-mRNA-1
chr6_2 three_prime_UTR 78491807 78492181 78491807 ID=Msa0929680-mRNA-1:three_prime_utr;Parent=Msa0929680-mRNA-1
Gene Sequence

>Msa0929680

ATGGTGGAGAAGACCGGCGCCGGTAGGAAGGAAGAGGTTGTCACCAGAGAGTACACCATCAACCTCCACAAACGCCTCCATGGCTGCACATTTAAGAAGAAAGCTCCTAAAGCAATAAAGGAGATCAGGAAGTTTGCCCAAAAGGCTATGGGAACTAATGATGTCAGAGTGGACGTCAAGTTGAACAAGGCTATTTGGAGCCAGGGGATCAGAAGTGTCCCTAGGAGGGTTAGGGTTCGCATTGCCCGCAAGAGGAACGATGATGAGGATGCTAAGGAAGAGCTCTACTCACTTGTTACTGTTGTTGAAATCCCCAAGGAAGAGCTCAAAGGATTGGGCACAAAGGTTATTGATGATGAGGATTGA
Protein sequence

>Msa0929680

MVEKTGAGRKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAMGTNDVRVDVKLNKAIWSQGIRSVPRRVRVRIARKRNDDEDAKEELYSLVTVVEIPKEELKGLGTKVIDDED*