AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0908870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0908870 MtrunA17_MTg0491251 98.544 206 3 0 1 206 8 213 9.43e-147 407
Msa0908870 MtrunA17_Chr7g0231341 95.455 176 8 0 31 206 6 181 1.65e-120 340
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTACTCGGCTCGGGTTC+GGG 0.146792 6_2:-9940923 Msa0908870:CDS
AAGCCGACGCCGACCCATTT+TGG 0.210676 6_2:+9940739 None:intergenic
CTCTTTGGATGTATCCTTCT+GGG 0.227156 6_2:-9940839 Msa0908870:CDS
CCTCTTTGGATGTATCCTTC+TGG 0.235353 6_2:-9940840 Msa0908870:CDS
AGACTTCGTTGCAAATAAAT+TGG 0.238736 6_2:-9940619 Msa0908870:CDS
CCCGCCGCTCCTATCCAAAA+TGG 0.239934 6_2:-9940753 Msa0908870:CDS
CTCTTTACTCGGCTCGGGTT+CGG 0.247183 6_2:-9940924 Msa0908870:CDS
TCAAAGAATGTCTAATTCTT+TGG 0.276750 6_2:-9941057 None:intergenic
TCAATAGAAATGCCAAAATA+AGG 0.312304 6_2:+9941009 None:intergenic
CGTAGCGCTTGCTCATAAAT+TGG 0.318064 6_2:+9940573 None:intergenic
AACCTCCTCTTTACTCGGCT+CGG 0.370554 6_2:-9940930 Msa0908870:CDS
ATTGGACCCTGCGGCTGCCC+CGG 0.373068 6_2:+9940591 None:intergenic
GATAGGAGCGGCGGGTCCGC+CGG 0.375170 6_2:+9940761 None:intergenic
GTTTAGAGACCTGCTGAAAA+AGG 0.379768 6_2:+9940950 None:intergenic
TATCCTTCTGGGGCTGATGC+TGG 0.380202 6_2:-9940828 Msa0908870:CDS
AGCGGCGGGTCCGCCGGGGC+GGG 0.390016 6_2:+9940767 None:intergenic
TATGGTTTCGCGGCCGGAGC+TGG 0.394590 6_2:+9940696 None:intergenic
GGGAAGGCTGATGCTGCTCT+TGG 0.402499 6_2:+9940787 None:intergenic
GCGGGTCCGCCGGGGCGGGA+AGG 0.403262 6_2:+9940771 None:intergenic
CTTTACTCGGCTCGGGTTCG+GGG 0.407970 6_2:-9940922 Msa0908870:CDS
GTGCCGAGGAAATCCCTCTT+TGG 0.408347 6_2:-9940854 Msa0908870:CDS
TTGGACCCTGCGGCTGCCCC+GGG 0.438206 6_2:+9940592 None:intergenic
GGAAGACCCGGGGCAGCCGC+AGG 0.439885 6_2:-9940598 Msa0908870:CDS
CCACTTTACTTTCAAAAGAT+CGG 0.450190 6_2:+9940974 None:intergenic
ACCTCCTCTTTACTCGGCTC+GGG 0.456204 6_2:-9940929 Msa0908870:CDS
TAGGAGCGGCGGGTCCGCCG+GGG 0.472146 6_2:+9940763 None:intergenic
TTCCGTGGAGCAGCCAGCTC+CGG 0.472407 6_2:-9940709 Msa0908870:CDS
GGCCGGAGCTGGCTGCTCCA+CGG 0.474217 6_2:+9940707 None:intergenic
CTCTAAACCTCCTCTTTACT+CGG 0.475617 6_2:-9940935 Msa0908870:CDS
TGCAAATAAATTGGAAGACC+CGG 0.488016 6_2:-9940610 Msa0908870:CDS
CAGCATCAGCCTTCCCGCCC+CGG 0.489074 6_2:-9940780 Msa0908870:CDS
CCGCCGCTCCTATCCAAAAT+GGG 0.493631 6_2:-9940752 Msa0908870:CDS
GGACGAACTTCAGCGCTGGC+TGG 0.506906 6_2:-9940508 Msa0908870:CDS
AGCGCTGGCTGGTTTCACTA+AGG 0.509513 6_2:-9940497 Msa0908870:CDS
AATACGATTGCCAAGGCGGC+CGG 0.515316 6_2:+9940897 None:intergenic
AGCTGAAGGAGGGCTATGTA+TGG 0.518841 6_2:+9940678 None:intergenic
CGAACCCGAGCCGAGTAAAG+AGG 0.520061 6_2:+9940925 None:intergenic
GCGCTGGCTGGTTTCACTAA+GGG 0.521276 6_2:-9940496 Msa0908870:CDS
GCTATGTATGGTTTCGCGGC+CGG 0.522221 6_2:+9940690 None:intergenic
GAGGGCTATGTATGGTTTCG+CGG 0.534222 6_2:+9940686 None:intergenic
AGCAAGCGCTACGCCTGGTA+TGG 0.535586 6_2:-9940563 Msa0908870:CDS
TTTACTCGGCTCGGGTTCGG+GGG 0.543290 6_2:-9940921 Msa0908870:CDS
TTATGAGCAAGCGCTACGCC+TGG 0.557111 6_2:-9940568 Msa0908870:CDS
CGCTCCTATCCAAAATGGGT+CGG 0.568858 6_2:-9940748 Msa0908870:CDS
GATCGATGGCAGTACTAACC+AGG 0.569954 6_2:-9940529 Msa0908870:CDS
GCAAATAAATTGGAAGACCC+GGG 0.573068 6_2:-9940609 Msa0908870:CDS
ATAGCCCTCCTTCAGCTAGA+GGG 0.580354 6_2:-9940672 Msa0908870:CDS
ACCAGGACGAACTTCAGCGC+TGG 0.584629 6_2:-9940512 Msa0908870:CDS
GAAGACCCGGGGCAGCCGCA+GGG 0.585751 6_2:-9940597 Msa0908870:CDS
GCCAGCGCTGAAGTTCGTCC+TGG 0.586980 6_2:+9940511 None:intergenic
TCTTTGGATGTATCCTTCTG+GGG 0.587601 6_2:-9940838 Msa0908870:CDS
TGGCTGCTCCACGGAACTAG+TGG 0.588921 6_2:+9940716 None:intergenic
AAGAAATACGATTGCCAAGG+CGG 0.590719 6_2:+9940893 None:intergenic
CAAAAGAAATACGATTGCCA+AGG 0.602322 6_2:+9940890 None:intergenic
AAAATGGATTTGTATTGTCG+GGG 0.604607 6_2:+9940468 None:intergenic
TCTCGAGTTCATACCATACC+AGG 0.605945 6_2:+9940550 None:intergenic
TGGTATGAACTCGAGATCGA+TGG 0.622194 6_2:-9940543 Msa0908870:CDS
CAGAAGGATACATCCAAAGA+GGG 0.626664 6_2:+9940841 None:intergenic
GCGAGCACTATTCAGTGCCG+AGG 0.627117 6_2:-9940868 Msa0908870:CDS
TAGCCCTCCTTCAGCTAGAG+GGG 0.628752 6_2:-9940671 Msa0908870:CDS
CATCCAAAGAGGGATTTCCT+CGG 0.631260 6_2:+9940851 None:intergenic
TATCCAAAATGGGTCGGCGT+CGG 0.631571 6_2:-9940742 Msa0908870:CDS
GAGCCAGCATCAGCCCCAGA+AGG 0.632838 6_2:+9940825 None:intergenic
CCAGAAGGATACATCCAAAG+AGG 0.635005 6_2:+9940840 None:intergenic
TGCTCATAAATTGGACCCTG+CGG 0.637696 6_2:+9940582 None:intergenic
CATAGCCCTCCTTCAGCTAG+AGG 0.642446 6_2:-9940673 Msa0908870:CDS
AGCCCTCCTTCAGCTAGAGG+GGG 0.644919 6_2:-9940670 Msa0908870:CDS
AAATGGATTTGTATTGTCGG+GGG 0.651927 6_2:+9940469 None:intergenic
ACCCGAGCCGAGTAAAGAGG+AGG 0.667486 6_2:+9940928 None:intergenic
CAAATAAATTGGAAGACCCG+GGG 0.684347 6_2:-9940608 Msa0908870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTTCTTCCTTATTT+TGG - chr6_2:9940469-9940488 Msa0908870:CDS 20.0%
! TCAATAGAAATGCCAAAATA+AGG + chr6_2:9940484-9940503 None:intergenic 25.0%
!! AAAAATGGATTTGTATTGTC+GGG + chr6_2:9941026-9941045 None:intergenic 25.0%
!! CAAAAATGGATTTGTATTGT+CGG + chr6_2:9941027-9941046 None:intergenic 25.0%
CCACTTTACTTTCAAAAGAT+CGG + chr6_2:9940519-9940538 None:intergenic 30.0%
AGACTTCGTTGCAAATAAAT+TGG - chr6_2:9940871-9940890 Msa0908870:CDS 30.0%
! AAAATGGATTTGTATTGTCG+GGG + chr6_2:9941025-9941044 None:intergenic 30.0%
! CGACAATACAAATCCATTTT+TGG - chr6_2:9941025-9941044 Msa0908870:CDS 30.0%
!! CCGATCTTTTGAAAGTAAAG+TGG - chr6_2:9940516-9940535 Msa0908870:CDS 35.0%
CAAAAGAAATACGATTGCCA+AGG + chr6_2:9940603-9940622 None:intergenic 35.0%
TGCAAATAAATTGGAAGACC+CGG - chr6_2:9940880-9940899 Msa0908870:CDS 35.0%
! AAATGGATTTGTATTGTCGG+GGG + chr6_2:9941024-9941043 None:intergenic 35.0%
!!! TAAAGTGGACCTTTTTCAGC+AGG - chr6_2:9940531-9940550 Msa0908870:CDS 40.0%
GTTTAGAGACCTGCTGAAAA+AGG + chr6_2:9940543-9940562 None:intergenic 40.0%
CTCTAAACCTCCTCTTTACT+CGG - chr6_2:9940555-9940574 Msa0908870:CDS 40.0%
AAGAAATACGATTGCCAAGG+CGG + chr6_2:9940600-9940619 None:intergenic 40.0%
CAGAAGGATACATCCAAAGA+GGG + chr6_2:9940652-9940671 None:intergenic 40.0%
CTCTTTGGATGTATCCTTCT+GGG - chr6_2:9940651-9940670 Msa0908870:CDS 40.0%
TCTTTGGATGTATCCTTCTG+GGG - chr6_2:9940652-9940671 Msa0908870:CDS 40.0%
GCAAATAAATTGGAAGACCC+GGG - chr6_2:9940881-9940900 Msa0908870:CDS 40.0%
CAAATAAATTGGAAGACCCG+GGG - chr6_2:9940882-9940901 Msa0908870:CDS 40.0%
!!! AATCCATTTTTGGCTTCTCC+CGG - chr6_2:9941035-9941054 Msa0908870:CDS 40.0%
CATCCAAAGAGGGATTTCCT+CGG + chr6_2:9940642-9940661 None:intergenic 45.0%
CCAGAAGGATACATCCAAAG+AGG + chr6_2:9940653-9940672 None:intergenic 45.0%
CCTCTTTGGATGTATCCTTC+TGG - chr6_2:9940650-9940669 Msa0908870:CDS 45.0%
TGCTCATAAATTGGACCCTG+CGG + chr6_2:9940911-9940930 None:intergenic 45.0%
CGTAGCGCTTGCTCATAAAT+TGG + chr6_2:9940920-9940939 None:intergenic 45.0%
TCTCGAGTTCATACCATACC+AGG + chr6_2:9940943-9940962 None:intergenic 45.0%
TGGTATGAACTCGAGATCGA+TGG - chr6_2:9940947-9940966 Msa0908870:CDS 45.0%
!!! ATCCATTTTTGGCTTCTCCC+GGG - chr6_2:9941036-9941055 Msa0908870:CDS 45.0%
AACCTCCTCTTTACTCGGCT+CGG - chr6_2:9940560-9940579 Msa0908870:CDS 50.0%
! CGACCCATTTTGGATAGGAG+CGG + chr6_2:9940744-9940763 None:intergenic 50.0%
CGCTCCTATCCAAAATGGGT+CGG - chr6_2:9940742-9940761 Msa0908870:CDS 50.0%
TATCCAAAATGGGTCGGCGT+CGG - chr6_2:9940748-9940767 Msa0908870:CDS 50.0%
GAGGGCTATGTATGGTTTCG+CGG + chr6_2:9940807-9940826 None:intergenic 50.0%
AGCTGAAGGAGGGCTATGTA+TGG + chr6_2:9940815-9940834 None:intergenic 50.0%
ATAGCCCTCCTTCAGCTAGA+GGG - chr6_2:9940818-9940837 Msa0908870:CDS 50.0%
GATCGATGGCAGTACTAACC+AGG - chr6_2:9940961-9940980 Msa0908870:CDS 50.0%
!!! TCCATTTTTGGCTTCTCCCG+GGG - chr6_2:9941037-9941056 Msa0908870:CDS 50.0%
ACCTCCTCTTTACTCGGCTC+GGG - chr6_2:9940561-9940580 Msa0908870:CDS 55.0%
!! CTCTTTACTCGGCTCGGGTT+CGG - chr6_2:9940566-9940585 Msa0908870:CDS 55.0%
!! TCTTTACTCGGCTCGGGTTC+GGG - chr6_2:9940567-9940586 Msa0908870:CDS 55.0%
AATACGATTGCCAAGGCGGC+CGG + chr6_2:9940596-9940615 None:intergenic 55.0%
GTGCCGAGGAAATCCCTCTT+TGG - chr6_2:9940636-9940655 Msa0908870:CDS 55.0%
!! TATCCTTCTGGGGCTGATGC+TGG - chr6_2:9940662-9940681 Msa0908870:CDS 55.0%
! CCATTTTGGATAGGAGCGGC+GGG + chr6_2:9940740-9940759 None:intergenic 55.0%
! CCCATTTTGGATAGGAGCGG+CGG + chr6_2:9940741-9940760 None:intergenic 55.0%
CCGCCGCTCCTATCCAAAAT+GGG - chr6_2:9940738-9940757 Msa0908870:CDS 55.0%
! GACGCCGACCCATTTTGGAT+AGG + chr6_2:9940749-9940768 None:intergenic 55.0%
GCTATGTATGGTTTCGCGGC+CGG + chr6_2:9940803-9940822 None:intergenic 55.0%
CATAGCCCTCCTTCAGCTAG+AGG - chr6_2:9940817-9940836 Msa0908870:CDS 55.0%
TAGCCCTCCTTCAGCTAGAG+GGG - chr6_2:9940819-9940838 Msa0908870:CDS 55.0%
TTATGAGCAAGCGCTACGCC+TGG - chr6_2:9940922-9940941 Msa0908870:CDS 55.0%
!! AGCGCTGGCTGGTTTCACTA+AGG - chr6_2:9940993-9941012 Msa0908870:CDS 55.0%
!! GCGCTGGCTGGTTTCACTAA+GGG - chr6_2:9940994-9941013 Msa0908870:CDS 55.0%
!!! CCATTTTTGGCTTCTCCCGG+GGG - chr6_2:9941038-9941057 Msa0908870:CDS 55.0%
!!! CATTTTTGGCTTCTCCCGGG+GGG - chr6_2:9941039-9941058 Msa0908870:CDS 55.0%
ACCCGAGCCGAGTAAAGAGG+AGG + chr6_2:9940565-9940584 None:intergenic 60.0%
CGAACCCGAGCCGAGTAAAG+AGG + chr6_2:9940568-9940587 None:intergenic 60.0%
!! CTTTACTCGGCTCGGGTTCG+GGG - chr6_2:9940568-9940587 Msa0908870:CDS 60.0%
!! TTTACTCGGCTCGGGTTCGG+GGG - chr6_2:9940569-9940588 Msa0908870:CDS 60.0%
GCGAGCACTATTCAGTGCCG+AGG - chr6_2:9940622-9940641 Msa0908870:CDS 60.0%
GGGAAGGCTGATGCTGCTCT+TGG + chr6_2:9940706-9940725 None:intergenic 60.0%
CCCGCCGCTCCTATCCAAAA+TGG - chr6_2:9940737-9940756 Msa0908870:CDS 60.0%
! AAGCCGACGCCGACCCATTT+TGG + chr6_2:9940754-9940773 None:intergenic 60.0%
! GTCGGCTTCCACTAGTTCCG+TGG - chr6_2:9940766-9940785 Msa0908870:CDS 60.0%
TGGCTGCTCCACGGAACTAG+TGG + chr6_2:9940777-9940796 None:intergenic 60.0%
AGCCCTCCTTCAGCTAGAGG+GGG - chr6_2:9940820-9940839 Msa0908870:CDS 60.0%
CTCCCCCTCTAGCTGAAGGA+GGG + chr6_2:9940825-9940844 None:intergenic 60.0%
TCCTTCAGCTAGAGGGGGAG+CGG - chr6_2:9940825-9940844 Msa0908870:CDS 60.0%
AGCAAGCGCTACGCCTGGTA+TGG - chr6_2:9940927-9940946 Msa0908870:CDS 60.0%
ACCAGGACGAACTTCAGCGC+TGG - chr6_2:9940978-9940997 Msa0908870:CDS 60.0%
CCCCCGGGAGAAGCCAAAAA+TGG + chr6_2:9941041-9941060 None:intergenic 60.0%
GAGCCAGCATCAGCCCCAGA+AGG + chr6_2:9940668-9940687 None:intergenic 65.0%
TTCCGTGGAGCAGCCAGCTC+CGG - chr6_2:9940781-9940800 Msa0908870:CDS 65.0%
TATGGTTTCGCGGCCGGAGC+TGG + chr6_2:9940797-9940816 None:intergenic 65.0%
GCTCCCCCTCTAGCTGAAGG+AGG + chr6_2:9940826-9940845 None:intergenic 65.0%
TCCGCTCCCCCTCTAGCTGA+AGG + chr6_2:9940829-9940848 None:intergenic 65.0%
! GCCAGCGCTGAAGTTCGTCC+TGG + chr6_2:9940982-9941001 None:intergenic 65.0%
!! GGACGAACTTCAGCGCTGGC+TGG - chr6_2:9940982-9941001 Msa0908870:CDS 65.0%
CAGCATCAGCCTTCCCGCCC+CGG - chr6_2:9940710-9940729 Msa0908870:CDS 70.0%
ATTGGACCCTGCGGCTGCCC+CGG + chr6_2:9940902-9940921 None:intergenic 70.0%
!! TCGGCTCGGGTTCGGGGGTC+CGG - chr6_2:9940574-9940593 Msa0908870:CDS 75.0%
CATCAGCCTTCCCGCCCCGG+CGG - chr6_2:9940713-9940732 Msa0908870:CDS 75.0%
ATAGGAGCGGCGGGTCCGCC+GGG + chr6_2:9940731-9940750 None:intergenic 75.0%
GATAGGAGCGGCGGGTCCGC+CGG + chr6_2:9940732-9940751 None:intergenic 75.0%
GGCCGGAGCTGGCTGCTCCA+CGG + chr6_2:9940786-9940805 None:intergenic 75.0%
GAAGACCCGGGGCAGCCGCA+GGG - chr6_2:9940893-9940912 Msa0908870:CDS 75.0%
TTGGACCCTGCGGCTGCCCC+GGG + chr6_2:9940901-9940920 None:intergenic 75.0%
! GTTCGGGGGTCCGGCCGCCT+TGG - chr6_2:9940583-9940602 Msa0908870:CDS 80.0%
! TAGGAGCGGCGGGTCCGCCG+GGG + chr6_2:9940730-9940749 None:intergenic 80.0%
! GGAAGACCCGGGGCAGCCGC+AGG - chr6_2:9940892-9940911 Msa0908870:CDS 80.0%
! GCGGGTCCGCCGGGGCGGGA+AGG + chr6_2:9940722-9940741 None:intergenic 90.0%
! AGCGGCGGGTCCGCCGGGGC+GGG + chr6_2:9940726-9940745 None:intergenic 90.0%
! GAGCGGCGGGTCCGCCGGGG+CGG + chr6_2:9940727-9940746 None:intergenic 90.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 9940440 9941072 9940440 ID=Msa0908870;Name=Msa0908870
chr6_2 mRNA 9940440 9941072 9940440 ID=Msa0908870-mRNA-1;Parent=Msa0908870;Name=Msa0908870-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|210
chr6_2 exon 9940440 9941072 9940440 ID=Msa0908870-mRNA-1:exon:2477;Parent=Msa0908870-mRNA-1
chr6_2 CDS 9940440 9941072 9940440 ID=Msa0908870-mRNA-1:cds;Parent=Msa0908870-mRNA-1
Gene Sequence

>Msa0908870

ATGTCTAATTCTTTGGTAAGAATTTCTATCATTTTTTTCTTCCTTATTTTGGCATTTCTATTGATTGACGTTGATTCCGATCTTTTGAAAGTAAAGTGGACCTTTTTCAGCAGGTCTCTAAACCTCCTCTTTACTCGGCTCGGGTTCGGGGGTCCGGCCGCCTTGGCAATCGTATTTCTTTTGCGAGCACTATTCAGTGCCGAGGAAATCCCTCTTTGGATGTATCCTTCTGGGGCTGATGCTGGCTCAGAAGCAAGCGTAAACCAAGAGCAGCATCAGCCTTCCCGCCCCGGCGGACCCGCCGCTCCTATCCAAAATGGGTCGGCGTCGGCTTCCACTAGTTCCGTGGAGCAGCCAGCTCCGGCCGCGAAACCATACATAGCCCTCCTTCAGCTAGAGGGGGAGCGGAAACGTCTTATCGACGATATTGTAGACTTCGTTGCAAATAAATTGGAAGACCCGGGGCAGCCGCAGGGTCCAATTTATGAGCAAGCGCTACGCCTGGTATGGTATGAACTCGAGATCGATGGCAGTACTAACCAGGACGAACTTCAGCGCTGGCTGGTTTCACTAAGGGAAAACCCCCGACAATACAAATCCATTTTTGGCTTCTCCCGGGGGGATTTTTTATGA

Protein sequence

>Msa0908870

MSNSLVRISIIFFFLILAFLLIDVDSDLLKVKWTFFSRSLNLLFTRLGFGGPAALAIVFLLRALFSAEEIPLWMYPSGADAGSEASVNQEQHQPSRPGGPAAPIQNGSASASTSSVEQPAPAAKPYIALLQLEGERKRLIDDIVDFVANKLEDPGQPQGPIYEQALRLVWYELEIDGSTNQDELQRWLVSLRENPRQYKSIFGFSRGDFL*