AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06986


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06986.t1 MTR_7g108830 98.148 162 3 0 1 162 1 162 1.35e-116 327
MS.gene06986.t1 MTR_8g058620 98.148 162 3 0 1 162 1 162 1.35e-116 327
MS.gene06986.t1 MTR_1g088640 66.667 168 49 3 1 162 1 167 3.51e-77 227
MS.gene06986.t1 MTR_1g088640 66.867 166 49 3 1 162 1 164 1.35e-76 225
MS.gene06986.t1 MTR_1g088620 89.855 69 7 0 94 162 218 286 1.15e-36 128
MS.gene06986.t1 MTR_1g087200 42.500 160 79 6 6 158 5 158 6.23e-33 114
MS.gene06986.t1 MTR_1g083950 40.741 162 89 4 1 159 1 158 6.80e-33 115
MS.gene06986.t1 MTR_1g054750 36.905 168 93 4 7 162 21 187 1.56e-29 107
MS.gene06986.t1 MTR_1g054765 38.506 174 88 5 7 162 2 174 3.61e-29 105
MS.gene06986.t1 MTR_5g065980 39.181 171 88 5 1 156 1 170 3.06e-26 98.2
MS.gene06986.t1 MTR_5g065980 38.824 170 88 5 1 156 1 168 7.26e-26 97.4
MS.gene06986.t1 MTR_5g065980 34.667 150 82 5 1 136 1 148 1.17e-13 65.9
MS.gene06986.t1 MTR_2g034200 28.144 167 92 4 12 162 9 163 1.54e-12 62.4
MS.gene06986.t1 MTR_5g077130 31.288 163 96 6 4 156 1 157 1.55e-12 62.4
MS.gene06986.t1 MTR_5g077130 33.333 156 87 7 12 156 9 158 2.29e-12 62.0
MS.gene06986.t1 MTR_4g064933 30.719 153 96 4 12 157 9 158 7.36e-12 60.5
MS.gene06986.t1 MTR_7g065770 32.911 158 96 5 6 156 3 157 8.92e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06986.t1 AT2G47710 67.284 162 51 2 1 161 1 161 8.06e-72 213
MS.gene06986.t1 AT3G11930 39.506 162 85 4 10 159 35 195 3.06e-34 119
MS.gene06986.t1 AT3G11930 39.264 163 85 4 10 159 35 196 4.54e-34 119
MS.gene06986.t1 AT3G11930 39.024 164 85 4 10 159 35 197 7.02e-34 118
MS.gene06986.t1 AT1G09740 42.767 159 80 4 10 159 10 166 6.91e-33 115
MS.gene06986.t1 AT1G09740 42.767 159 80 4 10 159 10 166 6.91e-33 115
MS.gene06986.t1 AT3G58450 39.103 156 84 5 11 159 33 184 6.23e-29 105
MS.gene06986.t1 AT3G11930 33.862 189 85 5 10 159 35 222 5.77e-28 103
MS.gene06986.t1 AT5G49050 45.833 120 62 3 1 119 33 150 1.25e-27 100
MS.gene06986.t1 AT3G58450 38.037 163 83 6 11 159 33 191 1.00e-26 100
MS.gene06986.t1 AT3G62550 38.323 167 83 6 1 157 1 157 2.90e-26 97.8
MS.gene06986.t1 AT1G68300 35.948 153 90 5 7 156 10 157 1.86e-22 88.2

Find 32 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGCACCATGCTTCTATTT+TGG 0.216579 7.3:-6304841 MS.gene06986:CDS
TGAGATCAGTGTTTGGGTTT+TGG 0.228710 7.3:+6304354 None:intergenic
TCTACTAATGTTGGCTTCAT+AGG 0.275135 7.3:-6305515 MS.gene06986:CDS
TGAATGGACTCTGGATCATT+TGG 0.356319 7.3:-6305597 MS.gene06986:CDS
ACAAGAACAAGTTTGAAGAT+TGG 0.363933 7.3:+6305557 None:intergenic
GTTGTAGGTAGTCATGGTTA+TGG 0.387360 7.3:-6304819 MS.gene06986:CDS
TTAGCACATACGCTCTTGAA+TGG 0.393024 7.3:-6305613 MS.gene06986:CDS
AGCCAGTGAGATCAGTGTTT+GGG 0.402587 7.3:+6304348 None:intergenic
TTCAAATCGGCCTCCACAAT+AGG 0.409491 7.3:+6305367 None:intergenic
ATTAGTAGAAGGAGAAGGTT+TGG 0.417086 7.3:+6305529 None:intergenic
AACAAGTTTGAAGATTGGAT+TGG 0.418326 7.3:+6305562 None:intergenic
CAGCCAGTGAGATCAGTGTT+TGG 0.432645 7.3:+6304347 None:intergenic
TATTAAAATTACCGATCTCT+TGG 0.443273 7.3:+6305300 None:intergenic
ATGGTTATGGAGCTATAAAA+AGG 0.449333 7.3:-6304806 MS.gene06986:intron
ACAAGTTTGAAGATTGGATT+GGG 0.449888 7.3:+6305563 None:intergenic
CCTTCTCCTTCTACTAATGT+TGG 0.462856 7.3:-6305524 MS.gene06986:CDS
ATGAAGCCAACATTAGTAGA+AGG 0.466396 7.3:+6305518 None:intergenic
CGCTGAGATATTACCTATTG+TGG 0.517301 7.3:-6305380 MS.gene06986:CDS
ATACGCTCTTGAATGGACTC+TGG 0.555617 7.3:-6305606 MS.gene06986:CDS
AGACGTAGTAGTGGAAGTTG+TGG 0.557524 7.3:-6304901 MS.gene06986:CDS
GTAGTAGTGGAAGTTGTGGA+TGG 0.560904 7.3:-6304897 MS.gene06986:CDS
AGAAATCTGCACCAAGAGAT+CGG 0.569017 7.3:-6305311 MS.gene06986:intron
CCAACATTAGTAGAAGGAGA+AGG 0.571033 7.3:+6305524 None:intergenic
AATGTTGGCTTCATAGGCCC+TGG 0.571494 7.3:-6305509 MS.gene06986:intron
AAATGTTCTTTGTGATGCTG+TGG 0.577056 7.3:-6304865 MS.gene06986:CDS
GAGAAGCAAGTGATGGTGGT+CGG 0.595765 7.3:-6305653 MS.gene06986:CDS
TCAGGTAAAAGACGTAGTAG+TGG 0.600049 7.3:-6304910 MS.gene06986:intron
TTCAGAGAAGCAAGTGATGG+TGG 0.623921 7.3:-6305657 MS.gene06986:CDS
CTACAACCAAAATAGAAGCA+TGG 0.624553 7.3:+6304835 None:intergenic
AGTTTCAGAGAAGCAAGTGA+TGG 0.653690 7.3:-6305660 MS.gene06986:CDS
TGAGATATTACCTATTGTGG+AGG 0.658611 7.3:-6305377 MS.gene06986:CDS
CACAATAGGTAATATCTCAG+CGG 0.686848 7.3:+6305381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTTCTTTCATTAAATAA+AGG + chr7.3:6304949-6304968 None:intergenic 15.0%
!!! TATTCTCTCATTTTTTATTT+TGG - chr7.3:6304748-6304767 MS.gene06986:intron 15.0%
!! AATATATATACAAACATACC+AGG + chr7.3:6304539-6304558 None:intergenic 20.0%
!! ATATATATACAAACATACCA+GGG + chr7.3:6304538-6304557 None:intergenic 20.0%
!! TAACTTATTATCAATGTCAA+TGG + chr7.3:6305372-6305391 None:intergenic 20.0%
! AGTCTGATAAATACTTATAC+TGG - chr7.3:6305303-6305322 MS.gene06986:intron 25.0%
! TATTAAAATTACCGATCTCT+TGG + chr7.3:6304730-6304749 None:intergenic 25.0%
! TTGTAACAATGTTTATTCAG+CGG - chr7.3:6304620-6304639 MS.gene06986:intron 25.0%
!!! ACTAGCTTTTTCTTTATATC+AGG - chr7.3:6305099-6305118 MS.gene06986:intron 25.0%
AACAAGTTTGAAGATTGGAT+TGG + chr7.3:6304468-6304487 None:intergenic 30.0%
AATGCAATAGCATGATAACT+GGG + chr7.3:6305264-6305283 None:intergenic 30.0%
ACAAGAACAAGTTTGAAGAT+TGG + chr7.3:6304473-6304492 None:intergenic 30.0%
ACAAGTTTGAAGATTGGATT+GGG + chr7.3:6304467-6304486 None:intergenic 30.0%
ACCAATATAATCTCAAACGT+AGG - chr7.3:6305516-6305535 MS.gene06986:CDS 30.0%
ATACTGTGTATGCATACATA+TGG - chr7.3:6305064-6305083 MS.gene06986:intron 30.0%
ATGGTTATGGAGCTATAAAA+AGG - chr7.3:6305221-6305240 MS.gene06986:intron 30.0%
ATGTAACTTTGCATTTGCTT+AGG + chr7.3:6304985-6305004 None:intergenic 30.0%
CCAATATAATCTCAAACGTA+GGG - chr7.3:6305517-6305536 MS.gene06986:CDS 30.0%
TGAAACTGCATTTATTTGCA+GGG - chr7.3:6305582-6305601 MS.gene06986:CDS 30.0%
TGTAACAATGTTTATTCAGC+GGG - chr7.3:6304621-6304640 MS.gene06986:intron 30.0%
TTGAAACTGCATTTATTTGC+AGG - chr7.3:6305581-6305600 MS.gene06986:CDS 30.0%
! AGTAGCAGTTCTTTTCAAAT+CGG + chr7.3:6304676-6304695 None:intergenic 30.0%
!! GATTGGTGGAAAGTTATTTT+TGG - chr7.3:6304855-6304874 MS.gene06986:CDS 30.0%
!!! GGAAAGTTATTTTTGGAGTT+TGG - chr7.3:6304862-6304881 MS.gene06986:CDS 30.0%
AAATGTTCTTTGTGATGCTG+TGG - chr7.3:6305162-6305181 MS.gene06986:intron 35.0%
ATGAAGCCAACATTAGTAGA+AGG + chr7.3:6304512-6304531 None:intergenic 35.0%
ATTAGTAGAAGGAGAAGGTT+TGG + chr7.3:6304501-6304520 None:intergenic 35.0%
CAATGCAATAGCATGATAAC+TGG + chr7.3:6305265-6305284 None:intergenic 35.0%
CACAATAGGTAATATCTCAG+CGG + chr7.3:6304649-6304668 None:intergenic 35.0%
CATTGCGATAATAGCGTTAA+AGG - chr7.3:6305280-6305299 MS.gene06986:intron 35.0%
CCTCTATATTCCTATGGATT+GGG + chr7.3:6304831-6304850 None:intergenic 35.0%
CTACAACCAAAATAGAAGCA+TGG + chr7.3:6305195-6305214 None:intergenic 35.0%
TCCTCTATATTCCTATGGAT+TGG + chr7.3:6304832-6304851 None:intergenic 35.0%
TGAGATATTACCTATTGTGG+AGG - chr7.3:6304650-6304669 MS.gene06986:intron 35.0%
TTGCATTTGCTTAGGAACTA+AGG + chr7.3:6304977-6304996 None:intergenic 35.0%
! CCCTACGTTTGAGATTATAT+TGG + chr7.3:6305520-6305539 None:intergenic 35.0%
!! TCTACTAATGTTGGCTTCAT+AGG - chr7.3:6304512-6304531 MS.gene06986:intron 35.0%
!! TTTATTTGCAGGGCTGTTTT+AGG - chr7.3:6305592-6305611 MS.gene06986:CDS 35.0%
!!! ATTTTGGTTGTAGGTAGTCA+TGG - chr7.3:6305202-6305221 MS.gene06986:intron 35.0%
!!! CATGCTTCTATTTTGGTTGT+AGG - chr7.3:6305193-6305212 MS.gene06986:intron 35.0%
AGAAATCTGCACCAAGAGAT+CGG - chr7.3:6304716-6304735 MS.gene06986:intron 40.0%
AGACTAATCTCCCAATCCAT+AGG - chr7.3:6304818-6304837 MS.gene06986:CDS 40.0%
AGGAATATAGAGGAGTGGAT+TGG - chr7.3:6304838-6304857 MS.gene06986:CDS 40.0%
AGTTTCAGAGAAGCAAGTGA+TGG - chr7.3:6304367-6304386 MS.gene06986:CDS 40.0%
CCAACATTAGTAGAAGGAGA+AGG + chr7.3:6304506-6304525 None:intergenic 40.0%
CCCAATCCATAGGAATATAG+AGG - chr7.3:6304828-6304847 MS.gene06986:CDS 40.0%
CCTTCTCCTTCTACTAATGT+TGG - chr7.3:6304503-6304522 MS.gene06986:intron 40.0%
CGCTGAGATATTACCTATTG+TGG - chr7.3:6304647-6304666 MS.gene06986:intron 40.0%
GTTGTAGGTAGTCATGGTTA+TGG - chr7.3:6305208-6305227 MS.gene06986:intron 40.0%
TAAAGGAAGAACAGAGCTAG+TGG + chr7.3:6304932-6304951 None:intergenic 40.0%
TCAGGTAAAAGACGTAGTAG+TGG - chr7.3:6305117-6305136 MS.gene06986:intron 40.0%
TCCACTCCTCTATATTCCTA+TGG + chr7.3:6304837-6304856 None:intergenic 40.0%
TCCATAGGAATATAGAGGAG+TGG - chr7.3:6304833-6304852 MS.gene06986:CDS 40.0%
TTAGCACATACGCTCTTGAA+TGG - chr7.3:6304414-6304433 MS.gene06986:CDS 40.0%
! TGAATGGACTCTGGATCATT+TGG - chr7.3:6304430-6304449 MS.gene06986:intron 40.0%
!! AATATAGAGGAGTGGATTGG+TGG - chr7.3:6304841-6304860 MS.gene06986:CDS 40.0%
!! GAAGCACCATGCTTCTATTT+TGG - chr7.3:6305186-6305205 MS.gene06986:intron 40.0%
AAGAACAGAGCTAGTGGAAG+TGG + chr7.3:6304926-6304945 None:intergenic 45.0%
ACACCTTCAACCTAAGGTGT+CGG - chr7.3:6305472-6305491 MS.gene06986:intron 45.0%
ACACTCACAATGTCAGACAC+TGG - chr7.3:6305447-6305466 MS.gene06986:intron 45.0%
TTCAAATCGGCCTCCACAAT+AGG + chr7.3:6304663-6304682 None:intergenic 45.0%
TTCAGAGAAGCAAGTGATGG+TGG - chr7.3:6304370-6304389 MS.gene06986:CDS 45.0%
! AGACGTAGTAGTGGAAGTTG+TGG - chr7.3:6305126-6305145 MS.gene06986:intron 45.0%
! ATACGCTCTTGAATGGACTC+TGG - chr7.3:6304421-6304440 MS.gene06986:CDS 45.0%
! GTAGTAGTGGAAGTTGTGGA+TGG - chr7.3:6305130-6305149 MS.gene06986:intron 45.0%
CTGGACACACCTTCAACCTA+AGG - chr7.3:6305466-6305485 MS.gene06986:intron 50.0%
!! AATGTTGGCTTCATAGGCCC+TGG - chr7.3:6304518-6304537 MS.gene06986:intron 50.0%
!! GAGAAGCAAGTGATGGTGGT+CGG - chr7.3:6304374-6304393 MS.gene06986:CDS 50.0%
!! GCACCGACACCTTAGGTTGA+AGG + chr7.3:6305478-6305497 None:intergenic 55.0%
!! GTACGTAGCACCGACACCTT+AGG + chr7.3:6305485-6305504 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 6304359 6305690 6304359 ID=MS.gene06986
chr7.3 mRNA 6304359 6305690 6304359 ID=MS.gene06986.t1;Parent=MS.gene06986
chr7.3 exon 6305510 6305690 6305510 ID=MS.gene06986.t1.exon1;Parent=MS.gene06986.t1
chr7.3 CDS 6305510 6305690 6305510 ID=cds.MS.gene06986.t1;Parent=MS.gene06986.t1
chr7.3 exon 6305312 6305409 6305312 ID=MS.gene06986.t1.exon2;Parent=MS.gene06986.t1
chr7.3 CDS 6305312 6305409 6305312 ID=cds.MS.gene06986.t1;Parent=MS.gene06986.t1
chr7.3 exon 6304807 6304928 6304807 ID=MS.gene06986.t1.exon3;Parent=MS.gene06986.t1
chr7.3 CDS 6304807 6304928 6304807 ID=cds.MS.gene06986.t1;Parent=MS.gene06986.t1
chr7.3 exon 6304359 6304446 6304359 ID=MS.gene06986.t1.exon4;Parent=MS.gene06986.t1
chr7.3 CDS 6304359 6304446 6304359 ID=cds.MS.gene06986.t1;Parent=MS.gene06986.t1
Gene Sequence

>MS.gene06986

ATGGCTCAAGTTTCAGAGAAGCAAGTGATGGTGGTCGGAGTCGACGACAGTGAATTTAGCACATACGCTCTTGAATGGACTCTGGATCATTTGGTTACTACACTTCCCAATCCAATCTTCAAACTTGTTCTTGTTTTTGCCAAACCTTCTCCTTCTACTAATGTTGGCTTCATAGGCCCTGGTATGTTTGTATATATATTGTTTGTTTCTTCTTAATTATGATATTTCATATTAAATGCGATCTCGTTGTTGATGATGATTTTGTAACAATGTTTATTCAGCGGGAGCCGCTGAGATATTACCTATTGTGGAGGCCGATTTGAAAAGAACTGCTACTATTGTTATTGAAAGAGCTCAAGAAATCTGCACCAAGAGATCGGTAATTTTAATATTCTCTCATTTTTTATTTTGGATGTTCATGCTAATTAATATGCATGATACATTGCATATAAGTAATCAAGACTAATCTCCCAATCCATAGGAATATAGAGGAGTGGATTGGTGGAAAGTTATTTTTGGAGTTTGGTGAAAAGATGAATAATAGTGAAAATTCATCATTTCAAACCACTTCCACTAGCTCTGTTCTTCCTTTATTTAATGAAAGAAAGAACTAATCCTTAGTTCCTAAGCAAATGCAAAGTTACATTTTACAGTTGATTATTTGATTTTACTTCATTTTTTGATGTCTCAGAATCAAAGTTCAATATACTGTGTATGCATACATATGGATTATCATCTGAACTAGCTTTTTCTTTATATCAGGTAAAAGACGTAGTAGTGGAAGTTGTGGATGGAGATGCTAGAAATGTTCTTTGTGATGCTGTGGAGAAGCACCATGCTTCTATTTTGGTTGTAGGTAGTCATGGTTATGGAGCTATAAAAAGGTATGTGTTCATTCAGATCCCAGTTATCATGCTATTGCATTGCGATAATAGCGTTAAAGGAGTCTGATAAATACTTATACTGGAACTTTCTAGTATTATCGAGCTATGAAGCACTGACATTGAACACCATTGACATTGATAATAAGTTAAGAAAATATAAATAATTTAATTTAATATCATGTGTTAGTTTCGTGTCATTGTCGAACACTCACAATGTCAGACACTGGACACACCTTCAACCTAAGGTGTCGGTGCTACGTACTTTTCAAGTGAACCAATATAATCTCAAACGTAGGGTCACGATATTTGTTTTTCAAATCTGTTAATTCACTTATGCTTTGAAACTGCATTTATTTGCAGGGCTGTTTTAGGTAGTGTAAGTGACTATTGTGCTCATCATGCTCATTGCACTGTGATGATCGTGAAGAAGCCAAAACCCAAACACTGA

Protein sequence

>MS.gene06986.t1

MAQVSEKQVMVVGVDDSEFSTYALEWTLDHLVTTLPNPIFKLVLVFAKPSPSTNVGFIGPAGAAEILPIVEADLKRTATIVIERAQEICTKRSVKDVVVEVVDGDARNVLCDAVEKHHASILVVGSHGYGAIKRAVLGSVSDYCAHHAHCTVMIVKKPKPKH