Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007681.t1 | XP_003606367.1 | 99 | 209 | 2 | 0 | 1 | 209 | 1 | 209 | 2.50E-111 | 411.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007681.t1 | Q8RUN2 | 90.8 | 196 | 18 | 0 | 9 | 204 | 4 | 199 | 1.2e-98 | 360.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007681.t1 | G7JND4 | 99.0 | 209 | 2 | 0 | 1 | 209 | 1 | 209 | 1.8e-111 | 411.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051577 | MS.gene007681 | PPI |
| MS.gene22428 | MS.gene007681 | PPI |
| MS.gene007681 | MS.gene52386 | PPI |
| MS.gene007681 | MS.gene20068 | PPI |
| MS.gene007681 | MS.gene055648 | PPI |
| MS.gene007681 | MS.gene029824 | PPI |
| MS.gene007681 | MS.gene016181 | PPI |
| MS.gene007681 | MS.gene034799 | PPI |
| MS.gene007681 | MS.gene24610 | PPI |
| MS.gene007681 | MS.gene41079 | PPI |
| MS.gene007681 | MS.gene33028 | PPI |
| MS.gene007681 | MS.gene016180 | PPI |
| MS.gene007681 | MS.gene007521 | PPI |
| MS.gene007681 | MS.gene007426 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007681.t1 | MTR_4g058740 | 99.043 | 209 | 2 | 0 | 1 | 209 | 1 | 209 | 9.93e-154 | 424 |
| MS.gene007681.t1 | MTR_2g094790 | 88.517 | 209 | 24 | 0 | 1 | 209 | 1 | 209 | 2.72e-138 | 385 |
| MS.gene007681.t1 | MTR_1g064260 | 87.255 | 204 | 26 | 0 | 1 | 204 | 1 | 204 | 8.21e-133 | 372 |
| MS.gene007681.t1 | MTR_7g101290 | 87.940 | 199 | 24 | 0 | 6 | 204 | 7 | 205 | 1.33e-130 | 366 |
| MS.gene007681.t1 | MTR_0041s0100 | 87.940 | 199 | 24 | 0 | 6 | 204 | 7 | 205 | 1.33e-130 | 366 |
| MS.gene007681.t1 | MTR_3g113710 | 72.222 | 216 | 53 | 1 | 1 | 209 | 3 | 218 | 4.57e-116 | 330 |
| MS.gene007681.t1 | MTR_1g057020 | 75.377 | 199 | 49 | 0 | 6 | 204 | 2 | 200 | 8.18e-113 | 321 |
| MS.gene007681.t1 | MTR_3g113710 | 75.532 | 188 | 46 | 0 | 1 | 188 | 3 | 190 | 1.03e-107 | 309 |
| MS.gene007681.t1 | MTR_1g015830 | 68.269 | 208 | 62 | 1 | 1 | 208 | 1 | 204 | 8.16e-105 | 301 |
| MS.gene007681.t1 | MTR_1g064260 | 88.514 | 148 | 17 | 0 | 57 | 204 | 1 | 148 | 6.02e-95 | 274 |
| MS.gene007681.t1 | MTR_1g015830 | 69.079 | 152 | 47 | 0 | 57 | 208 | 1 | 152 | 7.34e-76 | 226 |
| MS.gene007681.t1 | MTR_3g113710 | 74.126 | 143 | 30 | 1 | 74 | 209 | 10 | 152 | 5.39e-74 | 221 |
| MS.gene007681.t1 | MTR_1g024145 | 71.845 | 103 | 29 | 0 | 28 | 130 | 150 | 252 | 4.07e-48 | 158 |
| MS.gene007681.t1 | MTR_1g024850 | 72.857 | 70 | 19 | 0 | 10 | 79 | 14 | 83 | 3.47e-33 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007681.t1 | AT2G28305 | 90.816 | 196 | 18 | 0 | 9 | 204 | 4 | 199 | 7.92e-133 | 372 |
| MS.gene007681.t1 | AT2G37210 | 85.644 | 202 | 29 | 0 | 7 | 208 | 8 | 209 | 8.89e-131 | 367 |
| MS.gene007681.t1 | AT3G53450 | 82.857 | 210 | 35 | 1 | 1 | 209 | 1 | 210 | 1.81e-128 | 360 |
| MS.gene007681.t1 | AT2G37210 | 76.106 | 226 | 30 | 1 | 7 | 208 | 8 | 233 | 8.24e-125 | 352 |
| MS.gene007681.t1 | AT2G28305 | 92.778 | 180 | 13 | 0 | 9 | 188 | 4 | 183 | 4.67e-123 | 346 |
| MS.gene007681.t1 | AT5G06300 | 78.894 | 199 | 42 | 0 | 6 | 204 | 2 | 200 | 1.12e-117 | 333 |
| MS.gene007681.t1 | AT5G03270 | 79.793 | 193 | 39 | 0 | 3 | 195 | 9 | 201 | 6.03e-113 | 321 |
| MS.gene007681.t1 | AT5G03270 | 79.787 | 188 | 38 | 0 | 3 | 190 | 9 | 196 | 6.72e-109 | 310 |
| MS.gene007681.t1 | AT2G35990 | 69.849 | 199 | 60 | 0 | 6 | 204 | 2 | 200 | 9.30e-109 | 311 |
| MS.gene007681.t1 | AT4G35190 | 71.782 | 202 | 57 | 0 | 6 | 207 | 2 | 203 | 1.90e-108 | 310 |
| MS.gene007681.t1 | AT4G35190 | 73.545 | 189 | 50 | 0 | 6 | 194 | 2 | 190 | 4.80e-103 | 296 |
| MS.gene007681.t1 | AT5G11950 | 67.164 | 201 | 66 | 0 | 8 | 208 | 5 | 205 | 7.32e-103 | 296 |
| MS.gene007681.t1 | AT5G11950 | 67.164 | 201 | 66 | 0 | 8 | 208 | 5 | 205 | 7.32e-103 | 296 |
| MS.gene007681.t1 | AT5G11950 | 67.164 | 201 | 66 | 0 | 8 | 208 | 5 | 205 | 7.32e-103 | 296 |
| MS.gene007681.t1 | AT2G28305 | 92.647 | 136 | 10 | 0 | 9 | 144 | 4 | 139 | 8.41e-90 | 261 |
| MS.gene007681.t1 | AT2G35990 | 70.270 | 148 | 44 | 0 | 57 | 204 | 1 | 148 | 2.57e-78 | 232 |
| MS.gene007681.t1 | AT2G35990 | 70.270 | 148 | 44 | 0 | 57 | 204 | 1 | 148 | 2.57e-78 | 232 |
| MS.gene007681.t1 | AT5G11950 | 67.105 | 152 | 50 | 0 | 57 | 208 | 1 | 152 | 1.56e-74 | 222 |
| MS.gene007681.t1 | AT5G26140 | 55.102 | 147 | 44 | 1 | 84 | 208 | 6 | 152 | 2.97e-48 | 155 |
| MS.gene007681.t1 | AT5G26140 | 54.135 | 133 | 39 | 1 | 98 | 208 | 1 | 133 | 1.05e-41 | 138 |
| MS.gene007681.t1 | AT5G26140 | 58.824 | 102 | 42 | 0 | 107 | 208 | 17 | 118 | 1.22e-37 | 127 |
Find 52 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAATATTGATTTGGTTTA+TGG | 0.095929 | 4.3:-42576323 | MS.gene007681:CDS |
| ACAATTATATGACGAGCTTT+AGG | 0.212084 | 4.3:+42571765 | None:intergenic |
| TGTTAAGTACCTCCATCTTT+TGG | 0.280036 | 4.3:+42571715 | None:intergenic |
| CAATTATATGACGAGCTTTA+GGG | 0.317659 | 4.3:+42571766 | None:intergenic |
| TCTAAGCAAAGTTGGGAAAC+TGG | 0.328148 | 4.3:-42571198 | MS.gene007681:CDS |
| TGAAAGAATCAAAGTTTAAG+AGG | 0.352776 | 4.3:-42577783 | MS.gene007681:CDS |
| GGTGTCAAGAAATATTGATT+TGG | 0.365193 | 4.3:-42576330 | MS.gene007681:CDS |
| AGGAGGCAGCATTGGTTTGA+TGG | 0.380464 | 4.3:-42576300 | MS.gene007681:CDS |
| CAGCATTGGTTTGATGGGGT+TGG | 0.384600 | 4.3:-42576294 | MS.gene007681:CDS |
| GGAGGCAGCATTGGTTTGAT+GGG | 0.391809 | 4.3:-42576299 | MS.gene007681:CDS |
| GTTTCTCAAGCTGTTTATGA+TGG | 0.421989 | 4.3:-42576272 | MS.gene007681:CDS |
| CATCATAAAATTACCTCTCT+TGG | 0.431675 | 4.3:+42576103 | None:intergenic |
| TCTCTTGGCATCAGTGTCCT+TGG | 0.437369 | 4.3:+42576118 | None:intergenic |
| TATATGCATGAAGGTGGCTA+TGG | 0.441783 | 4.3:-42572028 | MS.gene007681:intron |
| GTTTATGGAGGAGGCAGCAT+TGG | 0.458836 | 4.3:-42576308 | MS.gene007681:CDS |
| GGAAACTTACTGGTTTATCA+TGG | 0.459986 | 4.3:+42571957 | None:intergenic |
| GATGCAACTCTTTCATGTTG+TGG | 0.462188 | 4.3:+42571219 | None:intergenic |
| AAAGATGCTGCTATTGAGCT+TGG | 0.470913 | 4.3:-42577715 | MS.gene007681:CDS |
| CTAAGCAAAGTTGGGAAACT+GGG | 0.478869 | 4.3:-42571197 | MS.gene007681:CDS |
| GTGGGATTGTTGAATGTAGA+TGG | 0.481596 | 4.3:-42571846 | MS.gene007681:CDS |
| AGCTTCTTGAGGTCATAACT+TGG | 0.481664 | 4.3:-42571994 | MS.gene007681:CDS |
| GATGGAGGTCGACATGTGAT+TGG | 0.485208 | 4.3:-42576254 | MS.gene007681:CDS |
| ATTGATTTGGTTTATGGAGG+AGG | 0.486097 | 4.3:-42576317 | MS.gene007681:CDS |
| AAGTTGGGAAACTGGGAAGC+TGG | 0.495777 | 4.3:-42571190 | MS.gene007681:CDS |
| AGTTGCATCTAAGCAAAGTT+GGG | 0.497061 | 4.3:-42571205 | MS.gene007681:CDS |
| AATAAAAGCATCAGAATGCT+TGG | 0.500098 | 4.3:+42574304 | None:intergenic |
| GAATGGGAACCAACCTGGTA+AGG | 0.500409 | 4.3:+42574280 | None:intergenic |
| GGAGAAGTGAAGGCAGTAGC+AGG | 0.500561 | 4.3:-42574353 | MS.gene007681:CDS |
| AGTGGGTTTGTGCAGATCAC+TGG | 0.500630 | 4.3:-42574386 | MS.gene007681:intron |
| GTCATAACTTGGGCTCAACT+TGG | 0.508031 | 4.3:-42571983 | MS.gene007681:CDS |
| AATATTGATTTGGTTTATGG+AGG | 0.510397 | 4.3:-42576320 | MS.gene007681:CDS |
| AAAAGAGCTTGTCCAAAAGA+TGG | 0.518338 | 4.3:-42571727 | MS.gene007681:CDS |
| AAGAAATAAAGAGATGGAGG+AGG | 0.519029 | 4.3:-42577815 | None:intergenic |
| GAGTTGCATCTAAGCAAAGT+TGG | 0.520245 | 4.3:-42571206 | MS.gene007681:CDS |
| GAGGCAGCATTGGTTTGATG+GGG | 0.537940 | 4.3:-42576298 | MS.gene007681:CDS |
| ATTGACAAAGCTGTGGAGGA+AGG | 0.543403 | 4.3:-42571798 | MS.gene007681:CDS |
| AGAGACAGTGGGAGAAGTGA+AGG | 0.543939 | 4.3:-42574363 | MS.gene007681:CDS |
| TCTCAAGCTGTTTATGATGG+AGG | 0.551173 | 4.3:-42576269 | MS.gene007681:CDS |
| TTATAGCTACTCTTGTTACC+AGG | 0.556974 | 4.3:+42577736 | None:intergenic |
| GCATCAAAGAAAAGCTGAGA+TGG | 0.557994 | 4.3:-42574327 | MS.gene007681:CDS |
| GACACTTGAGGAGCTTCTTG+AGG | 0.558323 | 4.3:-42572005 | MS.gene007681:CDS |
| ATGGAGGTCGACATGTGATT+GGG | 0.564949 | 4.3:-42576253 | MS.gene007681:intron |
| ATCTTTCATTGACAAAGCTG+TGG | 0.565707 | 4.3:-42571805 | MS.gene007681:CDS |
| GCTTCTTGAGGTCATAACTT+GGG | 0.575878 | 4.3:-42571993 | MS.gene007681:CDS |
| ATATGCATGAAGGTGGCTAT+GGG | 0.577892 | 4.3:-42572027 | MS.gene007681:intron |
| TATTGAGCTTGGAAATGAGT+TGG | 0.583903 | 4.3:-42577704 | MS.gene007681:intron |
| TTTCATTGACAAAGCTGTGG+AGG | 0.587801 | 4.3:-42571802 | MS.gene007681:CDS |
| CAGATCACTGGAGAGACAGT+GGG | 0.608222 | 4.3:-42574374 | MS.gene007681:CDS |
| GCAGATCACTGGAGAGACAG+TGG | 0.632705 | 4.3:-42574375 | MS.gene007681:intron |
| AAGGACACTGATGCCAAGAG+AGG | 0.647568 | 4.3:-42576116 | MS.gene007681:intron |
| AGGTGGCTATGGGACACTTG+AGG | 0.653214 | 4.3:-42572017 | MS.gene007681:CDS |
| AGAGCTTGTCCAAAAGATGG+AGG | 0.667572 | 4.3:-42571724 | MS.gene007681:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 42571185 | 42577824 | 42571185 | ID=MS.gene007681 |
| chr4.3 | mRNA | 42571185 | 42577824 | 42571185 | ID=MS.gene007681.t1;Parent=MS.gene007681 |
| chr4.3 | exon | 42577705 | 42577824 | 42577705 | ID=MS.gene007681.t1.exon1;Parent=MS.gene007681.t1 |
| chr4.3 | CDS | 42577705 | 42577824 | 42577705 | ID=cds.MS.gene007681.t1;Parent=MS.gene007681.t1 |
| chr4.3 | exon | 42576254 | 42576351 | 42576254 | ID=MS.gene007681.t1.exon2;Parent=MS.gene007681.t1 |
| chr4.3 | CDS | 42576254 | 42576351 | 42576254 | ID=cds.MS.gene007681.t1;Parent=MS.gene007681.t1 |
| chr4.3 | exon | 42576117 | 42576147 | 42576117 | ID=MS.gene007681.t1.exon3;Parent=MS.gene007681.t1 |
| chr4.3 | CDS | 42576117 | 42576147 | 42576117 | ID=cds.MS.gene007681.t1;Parent=MS.gene007681.t1 |
| chr4.3 | exon | 42574294 | 42574393 | 42574294 | ID=MS.gene007681.t1.exon4;Parent=MS.gene007681.t1 |
| chr4.3 | CDS | 42574294 | 42574393 | 42574294 | ID=cds.MS.gene007681.t1;Parent=MS.gene007681.t1 |
| chr4.3 | exon | 42571967 | 42572037 | 42571967 | ID=MS.gene007681.t1.exon5;Parent=MS.gene007681.t1 |
| chr4.3 | CDS | 42571967 | 42572037 | 42571967 | ID=cds.MS.gene007681.t1;Parent=MS.gene007681.t1 |
| chr4.3 | exon | 42571725 | 42571868 | 42571725 | ID=MS.gene007681.t1.exon6;Parent=MS.gene007681.t1 |
| chr4.3 | CDS | 42571725 | 42571868 | 42571725 | ID=cds.MS.gene007681.t1;Parent=MS.gene007681.t1 |
| chr4.3 | exon | 42571185 | 42571250 | 42571185 | ID=MS.gene007681.t1.exon7;Parent=MS.gene007681.t1 |
| chr4.3 | CDS | 42571185 | 42571250 | 42571185 | ID=cds.MS.gene007681.t1;Parent=MS.gene007681.t1 |
| Gene Sequence |
| Protein sequence |
