AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012420.t1 MTR_4g128750 98.266 173 3 0 1 173 1 173 2.43e-130 362
MS.gene012420.t1 MTR_4g128770 57.246 138 54 2 40 173 70 206 6.93e-54 170
MS.gene012420.t1 MTR_2g010600 44.509 173 83 3 3 173 1 162 3.61e-48 154
MS.gene012420.t1 MTR_4g050762 46.243 173 80 3 3 173 1 162 1.70e-47 153
MS.gene012420.t1 MTR_2g012370 46.243 173 80 3 3 173 1 162 1.70e-47 153
MS.gene012420.t1 MTR_2g435490 43.976 166 79 2 8 173 13 164 4.40e-47 152
MS.gene012420.t1 MTR_2g010590 44.444 171 83 3 3 173 1 159 3.74e-46 149
MS.gene012420.t1 MTR_2g010630 53.957 139 55 5 41 173 30 165 1.57e-43 142
MS.gene012420.t1 MTR_2g010650 48.555 173 73 7 6 173 4 165 2.12e-42 139
MS.gene012420.t1 MTR_4g092540 51.128 133 63 2 41 173 29 159 4.46e-40 133
MS.gene012420.t1 MTR_2g010580 49.624 133 64 3 41 173 35 164 1.22e-36 124
MS.gene012420.t1 MTR_2g010700 42.286 175 77 8 10 173 7 168 4.24e-34 118
MS.gene012420.t1 MTR_8g078770 37.349 166 100 2 11 173 8 172 9.48e-34 117
MS.gene012420.t1 MTR_1g067290 41.912 136 77 2 39 172 72 207 1.20e-32 116
MS.gene012420.t1 MTR_2g010610 54.639 97 42 2 77 173 44 138 1.72e-32 113
MS.gene012420.t1 MTR_5g018770 41.429 140 77 3 39 173 46 185 2.67e-32 114
MS.gene012420.t1 MTR_2g010640 50.420 119 53 5 57 173 49 163 5.49e-32 112
MS.gene012420.t1 MTR_2g010690 43.575 179 72 11 7 173 3 164 4.39e-31 110
MS.gene012420.t1 MTR_2g010670 41.477 176 80 9 7 173 3 164 1.12e-29 107
MS.gene012420.t1 MTR_5g018755 38.462 91 52 3 87 173 13 103 2.22e-15 68.6
MS.gene012420.t1 MTR_4g092515 42.222 90 43 2 7 95 6 87 3.36e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012420.t1 AT4G30320 53.529 170 64 3 5 173 6 161 3.97e-63 192
MS.gene012420.t1 AT5G57625 56.463 147 64 0 27 173 61 207 9.75e-60 185
MS.gene012420.t1 AT4G25790 57.971 138 58 0 36 173 73 210 5.55e-58 181
MS.gene012420.t1 AT4G25780 61.594 138 48 3 40 173 54 190 1.47e-56 176
MS.gene012420.t1 AT3G19690 56.338 142 58 3 34 173 22 161 5.10e-52 164
MS.gene012420.t1 AT1G01310 54.478 134 60 1 40 173 87 219 5.86e-48 156
MS.gene012420.t1 AT4G33720 47.590 166 74 3 8 173 11 163 1.36e-46 150
MS.gene012420.t1 AT4G31470 47.222 144 75 1 30 173 43 185 1.19e-45 148
MS.gene012420.t1 AT2G19990 43.182 176 94 3 1 173 4 176 2.11e-44 145
MS.gene012420.t1 AT2G14610 45.963 161 79 4 13 173 9 161 2.48e-44 144
MS.gene012420.t1 AT3G09590 47.101 138 72 1 37 173 49 186 3.42e-43 142
MS.gene012420.t1 AT2G14580 42.105 171 89 5 3 173 1 161 1.71e-42 139
MS.gene012420.t1 AT5G02730 46.667 135 71 1 40 173 59 193 2.98e-42 140
MS.gene012420.t1 AT4G33730 46.875 160 78 5 15 173 19 172 6.97e-42 138
MS.gene012420.t1 AT5G26130 47.853 163 77 4 13 173 10 166 2.15e-41 137
MS.gene012420.t1 AT1G50060 50.376 133 65 1 41 173 30 161 3.79e-38 128
MS.gene012420.t1 AT4G33710 46.528 144 73 3 32 173 25 166 4.63e-34 118
MS.gene012420.t1 AT1G50050 43.662 142 78 2 33 173 22 162 1.70e-32 114
MS.gene012420.t1 AT5G66590 40.876 137 77 2 41 173 49 185 1.87e-31 112
MS.gene012420.t1 AT1G50050 42.857 133 74 2 34 165 23 154 3.47e-28 105
MS.gene012420.t1 AT4G07820 37.324 142 85 4 32 173 23 160 4.30e-25 95.1
MS.gene012420.t1 AT2G19980 35.165 182 92 8 1 173 1 165 2.77e-22 88.2
MS.gene012420.t1 AT2G19970 34.161 161 86 7 26 173 24 177 1.46e-21 86.7

Find 42 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCGGCTCGAGCAATGTTC+TGG 0.228460 8.3:+2718263 None:intergenic
GACATTATACACAGGTTGTT+TGG 0.250521 8.3:-2718009 MS.gene012420:CDS
TTAGGCATTTCCATTTAATA+AGG 0.316056 8.3:+2717881 None:intergenic
ACATTATACACAGGTTGTTT+GGG 0.333299 8.3:-2718008 MS.gene012420:CDS
GATCCTCCTGGGAATTACTA+TGG 0.352062 8.3:-2717911 MS.gene012420:CDS
TGGCACAACTCTTGTGTTGA+TGG 0.370370 8.3:-2718043 MS.gene012420:CDS
CGCGGCTCGAGCAATGTTCT+GGG 0.390947 8.3:+2718264 None:intergenic
AGTTTATCATCCCACACTAA+TGG 0.395533 8.3:+2718229 None:intergenic
GAACATTCTAATGGTCCATA+TGG 0.400241 8.3:-2718154 MS.gene012420:CDS
ATGTGAGAAGTAGCAGAAAC+AGG 0.408625 8.3:+2718368 None:intergenic
CAGAAAATGTTCTCACCATA+TGG 0.409373 8.3:+2718139 None:intergenic
TGTGCTTTGGAACATTCTAA+TGG 0.435502 8.3:-2718163 MS.gene012420:CDS
ACATCCTCAAAATGAGCCTT+AGG 0.450766 8.3:-2718405 None:intergenic
AAGGCGTAACGACTGTGCTT+TGG 0.455806 8.3:-2718176 MS.gene012420:CDS
CAGCACAAGCAGTAAGTGCT+TGG 0.469294 8.3:-2718093 MS.gene012420:CDS
AACATTGCTCGAGCCGCGGT+GGG 0.474796 8.3:-2718259 MS.gene012420:CDS
CAAGCACTTACTGCTTGTGC+TGG 0.483462 8.3:+2718094 None:intergenic
TCTGGGGTAGTGGTGTTGGG+TGG 0.499740 8.3:-2718120 MS.gene012420:CDS
AACTAACACATTACGCGCAA+TGG 0.509259 8.3:-2718210 MS.gene012420:CDS
ACTTGTAACTATGATCCTCC+TGG 0.509796 8.3:-2717923 MS.gene012420:CDS
TGGGGTAGCACCACAAAAGT+TGG 0.510157 8.3:-2717989 MS.gene012420:CDS
AGCACAAGCAGTAAGTGCTT+GGG 0.519274 8.3:-2718092 MS.gene012420:CDS
CGCAATGGTACGCGAATCAA+AGG 0.521374 8.3:-2718195 MS.gene012420:CDS
GGATATAATTGGCACTCACA+TGG 0.522085 8.3:+2718338 None:intergenic
GAACATTGCTCGAGCCGCGG+TGG 0.532004 8.3:-2718260 MS.gene012420:CDS
CTCTCTCCATAGTAATTCCC+AGG 0.532209 8.3:+2717905 None:intergenic
TGGGGTTACGACCATTAGTG+TGG 0.555910 8.3:-2718240 MS.gene012420:CDS
GATATAATTGGCACTCACAT+GGG 0.586109 8.3:+2718339 None:intergenic
GGGCGAAACTAAAACACCTA+AGG 0.588985 8.3:+2718389 None:intergenic
GCGGCTCGAGCAATGTTCTG+GGG 0.599730 8.3:+2718265 None:intergenic
CTTGTAACTATGATCCTCCT+GGG 0.602594 8.3:-2717922 MS.gene012420:CDS
CCAGAACATTGCTCGAGCCG+CGG 0.607033 8.3:-2718263 MS.gene012420:CDS
ACATTGCTCGAGCCGCGGTG+GGG 0.613908 8.3:-2718258 MS.gene012420:CDS
TGTGAGAAGTAGCAGAAACA+GGG 0.632040 8.3:+2718369 None:intergenic
CGGCTCGAGCAATGTTCTGG+GGG 0.634408 8.3:+2718266 None:intergenic
GGGGTTACGACCATTAGTGT+GGG 0.642880 8.3:-2718239 MS.gene012420:CDS
GTTGTGTGTTCTGATGATAA+AGG 0.647257 8.3:-2717956 MS.gene012420:CDS
CATTATACACAGGTTGTTTG+GGG 0.647883 8.3:-2718007 MS.gene012420:CDS
TGTGTTGATGGAGAAATGTG+TGG 0.656782 8.3:-2718031 MS.gene012420:CDS
TCTCCATAGTAATTCCCAGG+AGG 0.722031 8.3:+2717908 None:intergenic
AATGTGTGGACATTATACAC+AGG 0.722792 8.3:-2718017 MS.gene012420:CDS
TAATGGTCGTAACCCCACCG+CGG 0.804220 8.3:+2718246 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAACAGTTTTACAATTAT+TGG - chr8.3:2718226-2718245 MS.gene012420:CDS 20.0%
!!! ATTTGAAACTTTTTCTGTTT+GGG + chr8.3:2717975-2717994 None:intergenic 20.0%
!!! GATTTGAAACTTTTTCTGTT+TGG + chr8.3:2717976-2717995 None:intergenic 25.0%
!!! TTTTCTGTTTGGGATATAAT+TGG + chr8.3:2717965-2717984 None:intergenic 25.0%
ACATTATACACAGGTTGTTT+GGG - chr8.3:2718281-2718300 MS.gene012420:CDS 30.0%
! ATATGGTGAGAACATTTTCT+GGG - chr8.3:2718152-2718171 MS.gene012420:CDS 30.0%
!!! AAATTGGTTTTTGAAGCTTC+TGG + chr8.3:2717998-2718017 None:intergenic 30.0%
AATGTGTGGACATTATACAC+AGG - chr8.3:2718272-2718291 MS.gene012420:CDS 35.0%
AGTTTATCATCCCACACTAA+TGG + chr8.3:2718063-2718082 None:intergenic 35.0%
CAGAAAATGTTCTCACCATA+TGG + chr8.3:2718153-2718172 None:intergenic 35.0%
CATTATACACAGGTTGTTTG+GGG - chr8.3:2718282-2718301 MS.gene012420:CDS 35.0%
GAACATTCTAATGGTCCATA+TGG - chr8.3:2718135-2718154 MS.gene012420:CDS 35.0%
GACATTATACACAGGTTGTT+TGG - chr8.3:2718280-2718299 MS.gene012420:CDS 35.0%
GATATAATTGGCACTCACAT+GGG + chr8.3:2717953-2717972 None:intergenic 35.0%
GTTGTGTGTTCTGATGATAA+AGG - chr8.3:2718333-2718352 MS.gene012420:CDS 35.0%
! CATATGGTGAGAACATTTTC+TGG - chr8.3:2718151-2718170 MS.gene012420:CDS 35.0%
! TATGGTGAGAACATTTTCTG+GGG - chr8.3:2718153-2718172 MS.gene012420:CDS 35.0%
!! TGTGCTTTGGAACATTCTAA+TGG - chr8.3:2718126-2718145 MS.gene012420:CDS 35.0%
AACTAACACATTACGCGCAA+TGG - chr8.3:2718079-2718098 MS.gene012420:CDS 40.0%
ACTTGTAACTATGATCCTCC+TGG - chr8.3:2718366-2718385 MS.gene012420:CDS 40.0%
ATGTGAGAAGTAGCAGAAAC+AGG + chr8.3:2717924-2717943 None:intergenic 40.0%
CTTGTAACTATGATCCTCCT+GGG - chr8.3:2718367-2718386 MS.gene012420:CDS 40.0%
GGATATAATTGGCACTCACA+TGG + chr8.3:2717954-2717973 None:intergenic 40.0%
TGTGAGAAGTAGCAGAAACA+GGG + chr8.3:2717923-2717942 None:intergenic 40.0%
! ACTAGCACATCCAACTTTTG+TGG + chr8.3:2718313-2718332 None:intergenic 40.0%
! TGTGTTGATGGAGAAATGTG+TGG - chr8.3:2718258-2718277 MS.gene012420:CDS 40.0%
AGCACAAGCAGTAAGTGCTT+GGG - chr8.3:2718197-2718216 MS.gene012420:CDS 45.0%
CTCTCTCCATAGTAATTCCC+AGG + chr8.3:2718387-2718406 None:intergenic 45.0%
GATCCTCCTGGGAATTACTA+TGG - chr8.3:2718378-2718397 MS.gene012420:CDS 45.0%
GGGCGAAACTAAAACACCTA+AGG + chr8.3:2717903-2717922 None:intergenic 45.0%
TCTCCATAGTAATTCCCAGG+AGG + chr8.3:2718384-2718403 None:intergenic 45.0%
TGGCACAACTCTTGTGTTGA+TGG - chr8.3:2718246-2718265 MS.gene012420:CDS 45.0%
!! TGTTCTGGGGGATCAGAAAT+TGG + chr8.3:2718014-2718033 None:intergenic 45.0%
!!! ATTTTCTGGGGTAGTGGTGT+TGG - chr8.3:2718165-2718184 MS.gene012420:CDS 45.0%
!!! GAGAACATTTTCTGGGGTAG+TGG - chr8.3:2718159-2718178 MS.gene012420:CDS 45.0%
!!! TTTTCTGGGGTAGTGGTGTT+GGG - chr8.3:2718166-2718185 MS.gene012420:CDS 45.0%
AAGGCGTAACGACTGTGCTT+TGG - chr8.3:2718113-2718132 MS.gene012420:CDS 50.0%
CAAGCACTTACTGCTTGTGC+TGG + chr8.3:2718198-2718217 None:intergenic 50.0%
CAGCACAAGCAGTAAGTGCT+TGG - chr8.3:2718196-2718215 MS.gene012420:CDS 50.0%
CGCAATGGTACGCGAATCAA+AGG - chr8.3:2718094-2718113 MS.gene012420:CDS 50.0%
GGGGTTACGACCATTAGTGT+GGG - chr8.3:2718050-2718069 MS.gene012420:CDS 50.0%
TGGGGTAGCACCACAAAAGT+TGG - chr8.3:2718300-2718319 MS.gene012420:CDS 50.0%
TGGGGTTACGACCATTAGTG+TGG - chr8.3:2718049-2718068 MS.gene012420:CDS 50.0%
TAATGGTCGTAACCCCACCG+CGG + chr8.3:2718046-2718065 None:intergenic 55.0%
AACATTGCTCGAGCCGCGGT+GGG - chr8.3:2718030-2718049 MS.gene012420:CDS 60.0%
CCAGAACATTGCTCGAGCCG+CGG - chr8.3:2718026-2718045 MS.gene012420:CDS 60.0%
CGCGGCTCGAGCAATGTTCT+GGG + chr8.3:2718028-2718047 None:intergenic 60.0%
CGGCTCGAGCAATGTTCTGG+GGG + chr8.3:2718026-2718045 None:intergenic 60.0%
GCGGCTCGAGCAATGTTCTG+GGG + chr8.3:2718027-2718046 None:intergenic 60.0%
! TCTGGGGTAGTGGTGTTGGG+TGG - chr8.3:2718169-2718188 MS.gene012420:CDS 60.0%
ACATTGCTCGAGCCGCGGTG+GGG - chr8.3:2718031-2718050 MS.gene012420:CDS 65.0%
CCGCGGCTCGAGCAATGTTC+TGG + chr8.3:2718029-2718048 None:intergenic 65.0%
GAACATTGCTCGAGCCGCGG+TGG - chr8.3:2718029-2718048 MS.gene012420:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 2717895 2718416 2717895 ID=MS.gene012420
chr8.3 mRNA 2717895 2718416 2717895 ID=MS.gene012420.t1;Parent=MS.gene012420
chr8.3 exon 2717895 2718416 2717895 ID=MS.gene012420.t1.exon1;Parent=MS.gene012420.t1
chr8.3 CDS 2717895 2718416 2717895 ID=cds.MS.gene012420.t1;Parent=MS.gene012420.t1
Gene Sequence

>MS.gene012420

ATGAGCCTTAGGTGTTTTAGTTTCGCCCTGTTTCTGCTACTTCTCACATTCATTTCCCATGTGAGTGCCAATTATATCCCAAACAGAAAAAGTTTCAAATCCAGAAGCTTCAAAAACCAATTTCTGATCCCCCAGAACATTGCTCGAGCCGCGGTGGGGTTACGACCATTAGTGTGGGATGATAAACTAACACATTACGCGCAATGGTACGCGAATCAAAGGCGTAACGACTGTGCTTTGGAACATTCTAATGGTCCATATGGTGAGAACATTTTCTGGGGTAGTGGTGTTGGGTGGAATCCAGCACAAGCAGTAAGTGCTTGGGTTGATGAGAAACAGTTTTACAATTATTGGCACAACTCTTGTGTTGATGGAGAAATGTGTGGACATTATACACAGGTTGTTTGGGGTAGCACCACAAAAGTTGGATGTGCTAGTGTTGTGTGTTCTGATGATAAAGGTACTTTCATGACTTGTAACTATGATCCTCCTGGGAATTACTATGGAGAGAGACCTTATTAA

Protein sequence

>MS.gene012420.t1

MSLRCFSFALFLLLLTFISHVSANYIPNRKSFKSRSFKNQFLIPQNIARAAVGLRPLVWDDKLTHYAQWYANQRRNDCALEHSNGPYGENIFWGSGVGWNPAQAVSAWVDEKQFYNYWHNSCVDGEMCGHYTQVVWGSTTKVGCASVVCSDDKGTFMTCNYDPPGNYYGERPY