AlfalfaGEDB | MS.gene028545

M. sativa cultivar XinJiangDaYe / MS.gene028545

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028545.t1	XP_024637244.1	97	164	5	0	1	164	1	164	2.30E-91	344.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028545.t1	P07053	61.0	159	61	1	6	164	11	168	5.2e-52	205.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028545.t1	A0A396IAF9	97.0	164	5	0	1	164	1	164	1.7e-91	344.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene028545	MS.gene012420	PPI
MS.gene028545	MS.gene02254	PPI
MS.gene028545	MS.gene035685	PPI
MS.gene028545	MS.gene01685	PPI
MS.gene028545	MS.gene038936	PPI
MS.gene028545	MS.gene57900	PPI
MS.gene028545	MS.gene00249	PPI
MS.gene028545	MS.gene02252	PPI
MS.gene028545	MS.gene002297	PPI
MS.gene028545	MS.gene018353	PPI
MS.gene028545	MS.gene01274	PPI
MS.gene028545	MS.gene002299	PPI
MS.gene028545	MS.gene01356	PPI
MS.gene028545	MS.gene002296	PPI
MS.gene028545	MS.gene01272	PPI
MS.gene028545	MS.gene01277	PPI
MS.gene028545	MS.gene01688	PPI
MS.gene028545	MS.gene02256	PPI
MS.gene028545	MS.gene01690	PPI
MS.gene028545	MS.gene02255	PPI
MS.gene028545	MS.gene01686	PPI
MS.gene028545	MS.gene002295	PPI
MS.gene028545	MS.gene035687	PPI
MS.gene028545	MS.gene002294	PPI
MS.gene028545	MS.gene01687	PPI
MS.gene028545	MS.gene01275	PPI
MS.gene028545	MS.gene01273	PPI
MS.gene028545	MS.gene02258	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028545.t1	MTR_2g010590	56.604	159	67	2	6	164	3	159	4.81e-63	191
MS.gene028545.t1	MTR_2g435490	55.828	163	67	3	3	164	6	164	5.57e-63	192
MS.gene028545.t1	MTR_4g092515	95.833	96	4	0	1	96	1	96	5.86e-63	190
MS.gene028545.t1	MTR_4g092540	54.902	153	68	1	12	164	8	159	1.86e-61	187
MS.gene028545.t1	MTR_2g010600	58.896	163	61	5	7	164	1	162	3.36e-60	184
MS.gene028545.t1	MTR_2g010650	53.571	168	68	6	2	164	3	165	2.62e-59	182
MS.gene028545.t1	MTR_2g010630	55.844	154	60	4	17	164	14	165	5.23e-58	179
MS.gene028545.t1	MTR_4g050762	60.000	145	54	3	23	164	19	162	2.95e-57	177
MS.gene028545.t1	MTR_2g012370	60.000	145	54	3	23	164	19	162	2.95e-57	177
MS.gene028545.t1	MTR_2g010580	49.390	164	79	3	1	164	5	164	7.16e-56	173
MS.gene028545.t1	MTR_2g010700	47.904	167	78	4	6	164	3	168	1.56e-51	162
MS.gene028545.t1	MTR_2g010640	46.951	164	79	5	6	164	3	163	1.24e-47	152
MS.gene028545.t1	MTR_2g010670	47.879	165	77	5	6	164	3	164	3.49e-45	146
MS.gene028545.t1	MTR_4g128750	48.936	141	71	1	25	164	33	173	3.92e-44	144
MS.gene028545.t1	MTR_2g010690	44.848	165	82	5	6	164	3	164	3.62e-43	141
MS.gene028545.t1	MTR_2g010610	48.951	143	49	4	23	164	19	138	4.41e-42	137
MS.gene028545.t1	MTR_4g128770	46.715	137	69	1	32	164	70	206	1.59e-38	130
MS.gene028545.t1	MTR_1g067290	37.500	144	87	2	23	163	64	207	4.59e-30	108
MS.gene028545.t1	MTR_8g078770	40.580	138	78	3	31	164	35	172	2.99e-28	103
MS.gene028545.t1	MTR_5g018770	34.946	186	98	6	1	164	1	185	8.50e-28	102
MS.gene028545.t1	MTR_5g018755	36.486	74	43	2	95	164	30	103	1.50e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028545.t1	AT1G50060	63.750	160	58	0	5	164	2	161	3.83e-72	214
MS.gene028545.t1	AT3G19690	57.419	155	62	3	12	164	9	161	2.54e-64	194
MS.gene028545.t1	AT4G33720	58.642	162	65	2	3	164	4	163	5.92e-63	191
MS.gene028545.t1	AT2G14580	53.988	163	69	3	2	164	5	161	2.08e-60	184
MS.gene028545.t1	AT1G50050	59.006	161	65	1	5	164	2	162	4.52e-58	179
MS.gene028545.t1	AT2G14610	51.220	164	77	2	1	164	1	161	3.70e-56	174
MS.gene028545.t1	AT5G26130	53.459	159	68	3	11	164	9	166	1.02e-53	168
MS.gene028545.t1	AT1G50050	58.940	151	61	1	5	154	2	152	2.31e-53	169
MS.gene028545.t1	AT2G19990	47.368	171	80	3	3	164	7	176	1.38e-51	162
MS.gene028545.t1	AT4G25790	54.815	135	60	1	31	164	76	210	2.27e-49	158
MS.gene028545.t1	AT5G57625	52.143	140	66	1	26	164	68	207	5.98e-48	154
MS.gene028545.t1	AT4G33710	54.225	142	62	2	25	164	26	166	1.30e-47	152
MS.gene028545.t1	AT4G33730	51.748	143	66	3	23	164	32	172	8.01e-47	150
MS.gene028545.t1	AT4G31470	49.254	134	68	0	31	164	52	185	5.68e-46	149
MS.gene028545.t1	AT3G09590	46.763	139	72	2	28	164	48	186	8.22e-42	138
MS.gene028545.t1	AT1G01310	46.617	133	71	0	32	164	87	219	1.16e-41	139
MS.gene028545.t1	AT4G30320	42.405	158	89	2	8	164	5	161	1.10e-40	134
MS.gene028545.t1	AT4G25780	46.715	137	69	2	32	164	54	190	1.21e-38	130
MS.gene028545.t1	AT5G02730	45.185	135	72	2	32	164	59	193	1.89e-38	130
MS.gene028545.t1	AT4G07820	45.385	130	69	2	25	154	24	151	1.15e-36	124
MS.gene028545.t1	AT5G66590	42.143	140	74	5	31	164	47	185	1.09e-27	102
MS.gene028545.t1	AT2G19970	37.349	166	89	6	2	154	3	166	4.59e-26	97.8
MS.gene028545.t1	AT2G19980	39.610	154	77	6	7	154	11	154	4.78e-25	95.1

Find 40 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTGTATAATGTTATTAAT+AGG	0.232687	4.1:-20651899	None:intergenic
TGAAAACCAAAGGGATAAAA+TGG	0.282665	4.1:-20651455	None:intergenic
TGGCGTAACTCTGTCCGTTT+AGG	0.318042	4.1:+20651793	MS.gene028545:CDS
AAGGAAAAGTGATTGCAATA+TGG	0.343884	4.1:+20651606	MS.gene028545:CDS
CTTTGAACTATGCAAATTCA+AGG	0.387653	4.1:+20651587	MS.gene028545:CDS
GGCGTAACTCTGTCCGTTTA+GGG	0.390384	4.1:+20651794	MS.gene028545:CDS
TCTGTATAATGTTATTAATA+GGG	0.390862	4.1:-20651898	None:intergenic
TGCAATATGGTTCACTCAAA+TGG	0.395818	4.1:+20651619	MS.gene028545:CDS
TTGGCGTTGCAAACATTACA+TGG	0.430593	4.1:+20651542	MS.gene028545:CDS
GGACCTTACGGTGAAAATCT+TGG	0.458251	4.1:+20651640	MS.gene028545:CDS
GGCTCAAGTGGTACATTCAC+TGG	0.463621	4.1:+20651667	MS.gene028545:CDS
GTACCCTAGCACACCCTAAA+CGG	0.466456	4.1:-20651807	None:intergenic
AACACTAATTCATGCATCAA+TGG	0.467730	4.1:+20651739	MS.gene028545:CDS
CTGTCCGTTTAGGGTGTGCT+AGG	0.470799	4.1:+20651803	MS.gene028545:CDS
GAGTTACTGCGGTAAATATG+TGG	0.489846	4.1:+20651689	MS.gene028545:CDS
GTTTGACCAACATAATTACC+AGG	0.515282	4.1:-20651874	None:intergenic
TTGTGCACGAGCTGTGTTGT+GGG	0.530104	4.1:-20651518	None:intergenic
GTTCACTCAAATGGACCTTA+CGG	0.557297	4.1:+20651628	MS.gene028545:CDS
AATCTTGGCAAAGGCTCAAG+TGG	0.583721	4.1:+20651655	MS.gene028545:CDS
TACGGTGAAAATCTTGGCAA+AGG	0.583835	4.1:+20651646	MS.gene028545:CDS
AGTGACAAACCACCATCCAT+TGG	0.585168	4.1:-20651836	None:intergenic
GGGATAAAATGGTCAAGAAG+AGG	0.590547	4.1:-20651444	None:intergenic
ATTGGAGAATGAAAACCAAA+GGG	0.591229	4.1:-20651464	None:intergenic
ATTTGCATAGTTCAAAGCAT+AGG	0.591735	4.1:-20651581	None:intergenic
CTTGTGCACGAGCTGTGTTG+TGG	0.595217	4.1:-20651519	None:intergenic
AACACAGCTCGTGCACAAGT+TGG	0.606318	4.1:+20651523	MS.gene028545:CDS
CATTGGAGAATGAAAACCAA+AGG	0.606530	4.1:-20651465	None:intergenic
GGGTACAATGTACCAATGGA+TGG	0.614628	4.1:+20651824	MS.gene028545:CDS
AGTTACTGCGGTAAATATGT+GGG	0.625164	4.1:+20651690	MS.gene028545:CDS
TGTCCGTTTAGGGTGTGCTA+GGG	0.646960	4.1:+20651804	MS.gene028545:CDS
TGCATTATACTCAAGTTGTG+TGG	0.648088	4.1:+20651773	MS.gene028545:CDS
GCTAGGGTACAATGTACCAA+TGG	0.658828	4.1:+20651820	MS.gene028545:CDS
TGGCGTTGCAAACATTACAT+GGG	0.667108	4.1:+20651543	MS.gene028545:CDS
TACAATGTACCAATGGATGG+TGG	0.668293	4.1:+20651827	MS.gene028545:CDS
ACTAATTCATGCATCAATGG+AGG	0.668457	4.1:+20651742	MS.gene028545:CDS
TTTGACCAACATAATTACCA+GGG	0.673673	4.1:-20651873	None:intergenic
TACATTCACTGGAGTTACTG+CGG	0.699180	4.1:+20651678	MS.gene028545:CDS
TGGGCGTCGAGAAAATCTTG+AGG	0.710833	4.1:-20651499	None:intergenic
TTGACCAACATAATTACCAG+GGG	0.712706	4.1:-20651872	None:intergenic
TGACCAACATAATTACCAGG+GGG	0.748433	4.1:-20651871	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACACTAATTCATGCATCAA+TGG	+	chr4.1:20651739-20651758	MS.gene028545:CDS	30.0%
	AAGGAAAAGTGATTGCAATA+TGG	+	chr4.1:20651606-20651625	MS.gene028545:CDS	30.0%
	ATTGGAGAATGAAAACCAAA+GGG	-	chr4.1:20651467-20651486	None:intergenic	30.0%
	ATTTGCATAGTTCAAAGCAT+AGG	-	chr4.1:20651584-20651603	None:intergenic	30.0%
	CTTTGAACTATGCAAATTCA+AGG	+	chr4.1:20651587-20651606	MS.gene028545:CDS	30.0%
	TGAAAACCAAAGGGATAAAA+TGG	-	chr4.1:20651458-20651477	None:intergenic	30.0%
	TTTGACCAACATAATTACCA+GGG	-	chr4.1:20651876-20651895	None:intergenic	30.0%
	ACTAATTCATGCATCAATGG+AGG	+	chr4.1:20651742-20651761	MS.gene028545:CDS	35.0%
	AGTTACTGCGGTAAATATGT+GGG	+	chr4.1:20651690-20651709	MS.gene028545:CDS	35.0%
	CATTGGAGAATGAAAACCAA+AGG	-	chr4.1:20651468-20651487	None:intergenic	35.0%
	GGCTAAAAAGTGAAAATGTG+TGG	-	chr4.1:20651426-20651445	None:intergenic	35.0%
	GTTTGACCAACATAATTACC+AGG	-	chr4.1:20651877-20651896	None:intergenic	35.0%
	TGCAATATGGTTCACTCAAA+TGG	+	chr4.1:20651619-20651638	MS.gene028545:CDS	35.0%
	TTGACCAACATAATTACCAG+GGG	-	chr4.1:20651875-20651894	None:intergenic	35.0%
!	TCTTGACCATTTTATCCCTT+TGG	+	chr4.1:20651449-20651468	MS.gene028545:CDS	35.0%
!	TGCATTATACTCAAGTTGTG+TGG	+	chr4.1:20651773-20651792	MS.gene028545:CDS	35.0%
!!	TTGAGGTAAGTTTTGTGCAT+TGG	-	chr4.1:20651485-20651504	None:intergenic	35.0%
	GAGTTACTGCGGTAAATATG+TGG	+	chr4.1:20651689-20651708	MS.gene028545:CDS	40.0%
	GGGATAAAATGGTCAAGAAG+AGG	-	chr4.1:20651447-20651466	None:intergenic	40.0%
	GTTCACTCAAATGGACCTTA+CGG	+	chr4.1:20651628-20651647	MS.gene028545:CDS	40.0%
	TACAATGTACCAATGGATGG+TGG	+	chr4.1:20651827-20651846	MS.gene028545:CDS	40.0%
	TACATTCACTGGAGTTACTG+CGG	+	chr4.1:20651678-20651697	MS.gene028545:CDS	40.0%
	TACGGTGAAAATCTTGGCAA+AGG	+	chr4.1:20651646-20651665	MS.gene028545:CDS	40.0%
	TGACCAACATAATTACCAGG+GGG	-	chr4.1:20651874-20651893	None:intergenic	40.0%
	TGGCGTTGCAAACATTACAT+GGG	+	chr4.1:20651543-20651562	MS.gene028545:CDS	40.0%
	TTGGCGTTGCAAACATTACA+TGG	+	chr4.1:20651542-20651561	MS.gene028545:CDS	40.0%
!	TTGCCAAGATTTTCACCGTA+AGG	-	chr4.1:20651646-20651665	None:intergenic	40.0%
	AATCTTGGCAAAGGCTCAAG+TGG	+	chr4.1:20651655-20651674	MS.gene028545:CDS	45.0%
	AGTGACAAACCACCATCCAT+TGG	-	chr4.1:20651839-20651858	None:intergenic	45.0%
	GATCCCCCTGGTAATTATGT+TGG	+	chr4.1:20651868-20651887	MS.gene028545:CDS	45.0%
	GCTAGGGTACAATGTACCAA+TGG	+	chr4.1:20651820-20651839	MS.gene028545:CDS	45.0%
	GGACCTTACGGTGAAAATCT+TGG	+	chr4.1:20651640-20651659	MS.gene028545:CDS	45.0%
	GGGTACAATGTACCAATGGA+TGG	+	chr4.1:20651824-20651843	MS.gene028545:CDS	45.0%
	AACACAGCTCGTGCACAAGT+TGG	+	chr4.1:20651523-20651542	MS.gene028545:CDS	50.0%
	ACTTGCAACTATGATCCCCC+TGG	+	chr4.1:20651856-20651875	MS.gene028545:CDS	50.0%
	GGCGTAACTCTGTCCGTTTA+GGG	+	chr4.1:20651794-20651813	MS.gene028545:CDS	50.0%
	GGCTCAAGTGGTACATTCAC+TGG	+	chr4.1:20651667-20651686	MS.gene028545:CDS	50.0%
	GTACCCTAGCACACCCTAAA+CGG	-	chr4.1:20651810-20651829	None:intergenic	50.0%
	TGGCGTAACTCTGTCCGTTT+AGG	+	chr4.1:20651793-20651812	MS.gene028545:CDS	50.0%
	TTGTGCACGAGCTGTGTTGT+GGG	-	chr4.1:20651521-20651540	None:intergenic	50.0%
!	TGTCCGTTTAGGGTGTGCTA+GGG	+	chr4.1:20651804-20651823	MS.gene028545:CDS	50.0%
!!	TGGGCGTCGAGAAAATCTTG+AGG	-	chr4.1:20651502-20651521	None:intergenic	50.0%
	CTTGTGCACGAGCTGTGTTG+TGG	-	chr4.1:20651522-20651541	None:intergenic	55.0%
!	CTGTCCGTTTAGGGTGTGCT+AGG	+	chr4.1:20651803-20651822	MS.gene028545:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	20651412	20651906	20651412	ID=MS.gene028545
chr4.1	mRNA	20651412	20651906	20651412	ID=MS.gene028545.t1;Parent=MS.gene028545
chr4.1	exon	20651412	20651906	20651412	ID=MS.gene028545.t1.exon1;Parent=MS.gene028545.t1
chr4.1	CDS	20651412	20651906	20651412	ID=cds.MS.gene028545.t1;Parent=MS.gene028545.t1

Gene Sequence

>MS.gene028545

ATGAGTTTCAACCACACATTTTCACTTTTTAGCCTCTTCTTGACCATTTTATCCCTTTGGTTTTCATTCTCCAATGCACAAAACTTACCTCAAGATTTTCTCGACGCCCACAACACAGCTCGTGCACAAGTTGGCGTTGCAAACATTACATGGGACAACACAGTTGCTACCTATGCTTTGAACTATGCAAATTCAAGGAAAAGTGATTGCAATATGGTTCACTCAAATGGACCTTACGGTGAAAATCTTGGCAAAGGCTCAAGTGGTACATTCACTGGAGTTACTGCGGTAAATATGTGGGTTAATGAAAAACAATACTATGATTATAACACTAATTCATGCATCAATGGAGGACAATGCTTGCATTATACTCAAGTTGTGTGGCGTAACTCTGTCCGTTTAGGGTGTGCTAGGGTACAATGTACCAATGGATGGTGGTTTGTCACTTGCAACTATGATCCCCCTGGTAATTATGTTGGTCAAACACCCTATTAA

Protein sequence

>MS.gene028545.t1

MSFNHTFSLFSLFLTILSLWFSFSNAQNLPQDFLDAHNTARAQVGVANITWDNTVATYALNYANSRKSDCNMVHSNGPYGENLGKGSSGTFTGVTAVNMWVNEKQYYDYNTNSCINGGQCLHYTQVVWRNSVRLGCARVQCTNGWWFVTCNYDPPGNYVGQTPY