AlfalfaGEDB | MS.gene018353

M. sativa cultivar XinJiangDaYe / MS.gene018353

KEGG: -
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018353.t1	XP_003610169.1	98.3	173	3	0	1	173	1	173	1.70E-100	375.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018353.t1	Q40374	98.3	173	3	0	1	173	1	173	4.0e-103	375.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018353.t1	G7JD28	98.3	173	3	0	1	173	1	173	1.2e-100	375.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene018353	MS.gene01356	PPI
MS.gene01272	MS.gene018353	PPI
MS.gene018353	MS.gene01277	PPI
MS.gene01688	MS.gene018353	PPI
MS.gene018353	MS.gene02256	PPI
MS.gene018353	MS.gene01690	PPI
MS.gene018353	MS.gene02255	PPI
MS.gene018353	MS.gene01686	PPI
MS.gene018353	MS.gene01685	PPI
MS.gene018353	MS.gene002295	PPI
MS.gene018353	MS.gene002294	PPI
MS.gene018353	MS.gene01275	PPI
MS.gene018353	MS.gene02258	PPI
MS.gene028545	MS.gene018353	PPI
MS.gene01687	MS.gene018353	PPI
MS.gene018353	MS.gene038936	PPI
MS.gene018353	MS.gene02252	PPI
MS.gene00249	MS.gene018353	PPI
MS.gene018353	MS.gene002296	PPI
MS.gene018353	MS.gene002297	PPI
MS.gene02254	MS.gene018353	PPI
MS.gene01273	MS.gene018353	PPI
MS.gene002299	MS.gene018353	PPI
MS.gene018353	MS.gene01274	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018353.t1	MTR_4g128750	98.266	173	3	0	1	173	1	173	2.43e-130	362
MS.gene018353.t1	MTR_4g128770	57.246	138	54	2	40	173	70	206	6.93e-54	170
MS.gene018353.t1	MTR_2g010600	44.509	173	83	3	3	173	1	162	3.61e-48	154
MS.gene018353.t1	MTR_4g050762	46.243	173	80	3	3	173	1	162	1.70e-47	153
MS.gene018353.t1	MTR_2g012370	46.243	173	80	3	3	173	1	162	1.70e-47	153
MS.gene018353.t1	MTR_2g435490	43.976	166	79	2	8	173	13	164	4.40e-47	152
MS.gene018353.t1	MTR_2g010590	44.444	171	83	3	3	173	1	159	3.74e-46	149
MS.gene018353.t1	MTR_2g010630	53.957	139	55	5	41	173	30	165	1.57e-43	142
MS.gene018353.t1	MTR_2g010650	48.555	173	73	7	6	173	4	165	2.12e-42	139
MS.gene018353.t1	MTR_4g092540	51.128	133	63	2	41	173	29	159	4.46e-40	133
MS.gene018353.t1	MTR_2g010580	49.624	133	64	3	41	173	35	164	1.22e-36	124
MS.gene018353.t1	MTR_2g010700	42.286	175	77	8	10	173	7	168	4.24e-34	118
MS.gene018353.t1	MTR_8g078770	37.349	166	100	2	11	173	8	172	9.48e-34	117
MS.gene018353.t1	MTR_1g067290	41.912	136	77	2	39	172	72	207	1.20e-32	116
MS.gene018353.t1	MTR_2g010610	54.639	97	42	2	77	173	44	138	1.72e-32	113
MS.gene018353.t1	MTR_5g018770	41.429	140	77	3	39	173	46	185	2.67e-32	114
MS.gene018353.t1	MTR_2g010640	50.420	119	53	5	57	173	49	163	5.49e-32	112
MS.gene018353.t1	MTR_2g010690	43.575	179	72	11	7	173	3	164	4.39e-31	110
MS.gene018353.t1	MTR_2g010670	41.477	176	80	9	7	173	3	164	1.12e-29	107
MS.gene018353.t1	MTR_5g018755	38.462	91	52	3	87	173	13	103	2.22e-15	68.6
MS.gene018353.t1	MTR_4g092515	42.222	90	43	2	7	95	6	87	3.36e-14	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018353.t1	AT4G30320	53.529	170	64	3	5	173	6	161	3.97e-63	192
MS.gene018353.t1	AT5G57625	56.463	147	64	0	27	173	61	207	9.75e-60	185
MS.gene018353.t1	AT4G25790	57.971	138	58	0	36	173	73	210	5.55e-58	181
MS.gene018353.t1	AT4G25780	61.594	138	48	3	40	173	54	190	1.47e-56	176
MS.gene018353.t1	AT3G19690	56.338	142	58	3	34	173	22	161	5.10e-52	164
MS.gene018353.t1	AT1G01310	54.478	134	60	1	40	173	87	219	5.86e-48	156
MS.gene018353.t1	AT4G33720	47.590	166	74	3	8	173	11	163	1.36e-46	150
MS.gene018353.t1	AT4G31470	47.222	144	75	1	30	173	43	185	1.19e-45	148
MS.gene018353.t1	AT2G19990	43.182	176	94	3	1	173	4	176	2.11e-44	145
MS.gene018353.t1	AT2G14610	45.963	161	79	4	13	173	9	161	2.48e-44	144
MS.gene018353.t1	AT3G09590	47.101	138	72	1	37	173	49	186	3.42e-43	142
MS.gene018353.t1	AT2G14580	42.105	171	89	5	3	173	1	161	1.71e-42	139
MS.gene018353.t1	AT5G02730	46.667	135	71	1	40	173	59	193	2.98e-42	140
MS.gene018353.t1	AT4G33730	46.875	160	78	5	15	173	19	172	6.97e-42	138
MS.gene018353.t1	AT5G26130	47.853	163	77	4	13	173	10	166	2.15e-41	137
MS.gene018353.t1	AT1G50060	50.376	133	65	1	41	173	30	161	3.79e-38	128
MS.gene018353.t1	AT4G33710	46.528	144	73	3	32	173	25	166	4.63e-34	118
MS.gene018353.t1	AT1G50050	43.662	142	78	2	33	173	22	162	1.70e-32	114
MS.gene018353.t1	AT5G66590	40.876	137	77	2	41	173	49	185	1.87e-31	112
MS.gene018353.t1	AT1G50050	42.857	133	74	2	34	165	23	154	3.47e-28	105
MS.gene018353.t1	AT4G07820	37.324	142	85	4	32	173	23	160	4.30e-25	95.1
MS.gene018353.t1	AT2G19980	35.165	182	92	8	1	173	1	165	2.77e-22	88.2
MS.gene018353.t1	AT2G19970	34.161	161	86	7	26	173	24	177	1.46e-21	86.7

Find 41 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGCGGCTCGAGCAATGTTC+TGG	0.228460	8.2:+5584148	None:intergenic
GACATTATACACAGGTTGTT+TGG	0.250521	8.2:-5583894	MS.gene018353:CDS
ACATTATACACAGGTTGTTT+GGG	0.333299	8.2:-5583893	MS.gene018353:CDS
GATCCTCCTGGGAATTACTA+TGG	0.352062	8.2:-5583796	MS.gene018353:CDS
TGGCACAACTCTTGTGTTGA+TGG	0.384290	8.2:-5583928	MS.gene018353:CDS
TTAGGCATTTCCATTCAATA+AGG	0.387633	8.2:+5583766	None:intergenic
CGCGGCTCGAGCAATGTTCT+GGG	0.390947	8.2:+5584149	None:intergenic
AGTTTATCATCCCACACTAA+TGG	0.395533	8.2:+5584114	None:intergenic
GAACATTCTAATGGTCCATA+TGG	0.400241	8.2:-5584039	MS.gene018353:CDS
CAGAAAATGTTCTCACCATA+TGG	0.409373	8.2:+5584024	None:intergenic
TGTGCTTTGGAACATTCTAA+TGG	0.435502	8.2:-5584048	MS.gene018353:CDS
ACATCCTCAAAATGAGCCTT+AGG	0.450766	8.2:-5584290	None:intergenic
AAGGCGTAACGACTGTGCTT+TGG	0.455806	8.2:-5584061	MS.gene018353:CDS
CAGCACAAGCAGTAAGTGCT+TGG	0.469294	8.2:-5583978	MS.gene018353:CDS
AACATTGCTCGAGCCGCGGT+GGG	0.474796	8.2:-5584144	MS.gene018353:CDS
CAAGCACTTACTGCTTGTGC+TGG	0.483462	8.2:+5583979	None:intergenic
TGGGGTAGCACTACAAAAGT+TGG	0.498560	8.2:-5583874	MS.gene018353:CDS
TCTGGGGTAGTGGTGTTGGG+TGG	0.499740	8.2:-5584005	MS.gene018353:CDS
ACATGTAACTATGATCCTCC+TGG	0.506469	8.2:-5583808	MS.gene018353:CDS
AACTAACACATTACGCGCAA+TGG	0.509259	8.2:-5584095	MS.gene018353:CDS
AGCACAAGCAGTAAGTGCTT+GGG	0.519274	8.2:-5583977	MS.gene018353:CDS
CGCAATGGTACGCGAATCAA+AGG	0.521374	8.2:-5584080	MS.gene018353:CDS
GGATATAATTGGCACTCACA+TGG	0.522085	8.2:+5584223	None:intergenic
GAACATTGCTCGAGCCGCGG+TGG	0.532004	8.2:-5584145	MS.gene018353:CDS
CTCTCTCCATAGTAATTCCC+AGG	0.532209	8.2:+5583790	None:intergenic
TGGGGTTACGACCATTAGTG+TGG	0.555910	8.2:-5584125	MS.gene018353:CDS
GATATAATTGGCACTCACAT+GGG	0.586109	8.2:+5584224	None:intergenic
AGGCGAAACTGAAACACCTA+AGG	0.597035	8.2:+5584274	None:intergenic
GCGGCTCGAGCAATGTTCTG+GGG	0.599730	8.2:+5584150	None:intergenic
CATGTAACTATGATCCTCCT+GGG	0.602594	8.2:-5583807	MS.gene018353:CDS
CCAGAACATTGCTCGAGCCG+CGG	0.607033	8.2:-5584148	MS.gene018353:CDS
ACATTGCTCGAGCCGCGGTG+GGG	0.613908	8.2:-5584143	MS.gene018353:CDS
CGGCTCGAGCAATGTTCTGG+GGG	0.634408	8.2:+5584151	None:intergenic
GGGGTTACGACCATTAGTGT+GGG	0.642880	8.2:-5584124	MS.gene018353:CDS
GTTGTGTGTTCTGATGATAA+AGG	0.647257	8.2:-5583841	MS.gene018353:CDS
CATTATACACAGGTTGTTTG+GGG	0.647883	8.2:-5583892	MS.gene018353:CDS
TGTGTTGATGGAGAAATGTG+TGG	0.656782	8.2:-5583916	MS.gene018353:CDS
TGTGAGAAGTAACAGAAACA+AGG	0.687417	8.2:+5584254	None:intergenic
TCTCCATAGTAATTCCCAGG+AGG	0.722031	8.2:+5583793	None:intergenic
AATGTGTGGACATTATACAC+AGG	0.722792	8.2:-5583902	MS.gene018353:CDS
TAATGGTCGTAACCCCACCG+CGG	0.804220	8.2:+5584131	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTGAAACTTTTTCTGTTT+GGG	+	chr8.2:5583860-5583879	None:intergenic	20.0%
!!	AGAAACAGTTTTACAATTAC+TGG	-	chr8.2:5584111-5584130	MS.gene018353:CDS	25.0%
!!!	GATTTGAAACTTTTTCTGTT+TGG	+	chr8.2:5583861-5583880	None:intergenic	25.0%
!!!	TTTTCTGTTTGGGATATAAT+TGG	+	chr8.2:5583850-5583869	None:intergenic	25.0%
	ACATTATACACAGGTTGTTT+GGG	-	chr8.2:5584166-5584185	MS.gene018353:CDS	30.0%
!	ATATGGTGAGAACATTTTCT+GGG	-	chr8.2:5584037-5584056	MS.gene018353:CDS	30.0%
!!!	AAATTGGTTTTTGAAGCTTC+TGG	+	chr8.2:5583883-5583902	None:intergenic	30.0%
	AATGTGTGGACATTATACAC+AGG	-	chr8.2:5584157-5584176	MS.gene018353:CDS	35.0%
	AGTTTATCATCCCACACTAA+TGG	+	chr8.2:5583948-5583967	None:intergenic	35.0%
	CAGAAAATGTTCTCACCATA+TGG	+	chr8.2:5584038-5584057	None:intergenic	35.0%
	CATTATACACAGGTTGTTTG+GGG	-	chr8.2:5584167-5584186	MS.gene018353:CDS	35.0%
	GAACATTCTAATGGTCCATA+TGG	-	chr8.2:5584020-5584039	MS.gene018353:CDS	35.0%
	GACATTATACACAGGTTGTT+TGG	-	chr8.2:5584165-5584184	MS.gene018353:CDS	35.0%
	GATATAATTGGCACTCACAT+GGG	+	chr8.2:5583838-5583857	None:intergenic	35.0%
	GTTGTGTGTTCTGATGATAA+AGG	-	chr8.2:5584218-5584237	MS.gene018353:CDS	35.0%
	TGTGAGAAGTAACAGAAACA+AGG	+	chr8.2:5583808-5583827	None:intergenic	35.0%
!	CATATGGTGAGAACATTTTC+TGG	-	chr8.2:5584036-5584055	MS.gene018353:CDS	35.0%
!	TATGGTGAGAACATTTTCTG+GGG	-	chr8.2:5584038-5584057	MS.gene018353:CDS	35.0%
!!	TGTGCTTTGGAACATTCTAA+TGG	-	chr8.2:5584011-5584030	MS.gene018353:CDS	35.0%
	AACTAACACATTACGCGCAA+TGG	-	chr8.2:5583964-5583983	MS.gene018353:CDS	40.0%
	ACATGTAACTATGATCCTCC+TGG	-	chr8.2:5584251-5584270	MS.gene018353:CDS	40.0%
	CATGTAACTATGATCCTCCT+GGG	-	chr8.2:5584252-5584271	MS.gene018353:CDS	40.0%
	GGATATAATTGGCACTCACA+TGG	+	chr8.2:5583839-5583858	None:intergenic	40.0%
!	TGTGTTGATGGAGAAATGTG+TGG	-	chr8.2:5584143-5584162	MS.gene018353:CDS	40.0%
	AGCACAAGCAGTAAGTGCTT+GGG	-	chr8.2:5584082-5584101	MS.gene018353:CDS	45.0%
	AGGCGAAACTGAAACACCTA+AGG	+	chr8.2:5583788-5583807	None:intergenic	45.0%
	CTCTCTCCATAGTAATTCCC+AGG	+	chr8.2:5584272-5584291	None:intergenic	45.0%
	GATCCTCCTGGGAATTACTA+TGG	-	chr8.2:5584263-5584282	MS.gene018353:CDS	45.0%
	TCTCCATAGTAATTCCCAGG+AGG	+	chr8.2:5584269-5584288	None:intergenic	45.0%
	TGGCACAACTCTTGTGTTGA+TGG	-	chr8.2:5584131-5584150	MS.gene018353:CDS	45.0%
	TGGGGTAGCACTACAAAAGT+TGG	-	chr8.2:5584185-5584204	MS.gene018353:CDS	45.0%
!!	TGTTCTGGGGGATCAGAAAT+TGG	+	chr8.2:5583899-5583918	None:intergenic	45.0%
!!!	ATTTTCTGGGGTAGTGGTGT+TGG	-	chr8.2:5584050-5584069	MS.gene018353:CDS	45.0%
!!!	GAGAACATTTTCTGGGGTAG+TGG	-	chr8.2:5584044-5584063	MS.gene018353:CDS	45.0%
!!!	TTTTCTGGGGTAGTGGTGTT+GGG	-	chr8.2:5584051-5584070	MS.gene018353:CDS	45.0%
	AAGGCGTAACGACTGTGCTT+TGG	-	chr8.2:5583998-5584017	MS.gene018353:CDS	50.0%
	CAAGCACTTACTGCTTGTGC+TGG	+	chr8.2:5584083-5584102	None:intergenic	50.0%
	CAGCACAAGCAGTAAGTGCT+TGG	-	chr8.2:5584081-5584100	MS.gene018353:CDS	50.0%
	CGCAATGGTACGCGAATCAA+AGG	-	chr8.2:5583979-5583998	MS.gene018353:CDS	50.0%
	GGGGTTACGACCATTAGTGT+GGG	-	chr8.2:5583935-5583954	MS.gene018353:CDS	50.0%
	TGGGGTTACGACCATTAGTG+TGG	-	chr8.2:5583934-5583953	MS.gene018353:CDS	50.0%
	TAATGGTCGTAACCCCACCG+CGG	+	chr8.2:5583931-5583950	None:intergenic	55.0%
	AACATTGCTCGAGCCGCGGT+GGG	-	chr8.2:5583915-5583934	MS.gene018353:CDS	60.0%
	CCAGAACATTGCTCGAGCCG+CGG	-	chr8.2:5583911-5583930	MS.gene018353:CDS	60.0%
	CGCGGCTCGAGCAATGTTCT+GGG	+	chr8.2:5583913-5583932	None:intergenic	60.0%
	CGGCTCGAGCAATGTTCTGG+GGG	+	chr8.2:5583911-5583930	None:intergenic	60.0%
	GCGGCTCGAGCAATGTTCTG+GGG	+	chr8.2:5583912-5583931	None:intergenic	60.0%
!	TCTGGGGTAGTGGTGTTGGG+TGG	-	chr8.2:5584054-5584073	MS.gene018353:CDS	60.0%
	ACATTGCTCGAGCCGCGGTG+GGG	-	chr8.2:5583916-5583935	MS.gene018353:CDS	65.0%
	CCGCGGCTCGAGCAATGTTC+TGG	+	chr8.2:5583914-5583933	None:intergenic	65.0%
	GAACATTGCTCGAGCCGCGG+TGG	-	chr8.2:5583914-5583933	MS.gene018353:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	5583780	5584301	5583780	ID=MS.gene018353
chr8.2	mRNA	5583780	5584301	5583780	ID=MS.gene018353.t1;Parent=MS.gene018353
chr8.2	exon	5583780	5584301	5583780	ID=MS.gene018353.t1.exon1;Parent=MS.gene018353.t1
chr8.2	CDS	5583780	5584301	5583780	ID=cds.MS.gene018353.t1;Parent=MS.gene018353.t1

Gene Sequence

>MS.gene018353

ATGAGCCTTAGGTGTTTCAGTTTCGCCTTGTTTCTGTTACTTCTCACATTCATTTCCCATGTGAGTGCCAATTATATCCCAAACAGAAAAAGTTTCAAATCCAGAAGCTTCAAAAACCAATTTCTGATCCCCCAGAACATTGCTCGAGCCGCGGTGGGGTTACGACCATTAGTGTGGGATGATAAACTAACACATTACGCGCAATGGTACGCGAATCAAAGGCGTAACGACTGTGCTTTGGAACATTCTAATGGTCCATATGGTGAGAACATTTTCTGGGGTAGTGGTGTTGGGTGGAATCCAGCACAAGCAGTAAGTGCTTGGGTTGATGAGAAACAGTTTTACAATTACTGGCACAACTCTTGTGTTGATGGAGAAATGTGTGGACATTATACACAGGTTGTTTGGGGTAGCACTACAAAAGTTGGATGTGCTAGTGTTGTGTGTTCTGATGATAAAGGTACTTTCATGACATGTAACTATGATCCTCCTGGGAATTACTATGGAGAGAGACCTTATTGA

Protein sequence

>MS.gene018353.t1

MSLRCFSFALFLLLLTFISHVSANYIPNRKSFKSRSFKNQFLIPQNIARAAVGLRPLVWDDKLTHYAQWYANQRRNDCALEHSNGPYGENIFWGSGVGWNPAQAVSAWVDEKQFYNYWHNSCVDGEMCGHYTQVVWGSTTKVGCASVVCSDDKGTFMTCNYDPPGNYYGERPY