Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025114.t1 | RDX65489.1 | 61.8 | 508 | 144 | 6 | 5 | 467 | 49 | 551 | 3.60E-169 | 604.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025114.t1 | A0A371EHW3 | 61.8 | 508 | 144 | 6 | 5 | 467 | 49 | 551 | 2.6e-169 | 604.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene026086 | MS.gene025114 | PPI |
| MS.gene025114 | MS.gene026085 | PPI |
| MS.gene025114 | MS.gene85627 | PPI |
| MS.gene025114 | MS.gene025116 | PPI |
| MS.gene025114 | MS.gene85625 | PPI |
| MS.gene025118 | MS.gene025114 | PPI |
| MS.gene012270 | MS.gene025114 | PPI |
| MS.gene025114 | MS.gene059902 | PPI |
| MS.gene84672 | MS.gene025114 | PPI |
| MS.gene025114 | MS.gene012270 | PPI |
| MS.gene57567 | MS.gene025114 | PPI |
| MS.gene051858 | MS.gene025114 | PPI |
| MS.gene57569 | MS.gene025114 | PPI |
| MS.gene025115 | MS.gene025114 | PPI |
| MS.gene85626 | MS.gene025114 | PPI |
| MS.gene025114 | MS.gene83168 | PPI |
| MS.gene025114 | MS.gene026088 | PPI |
| MS.gene025114 | MS.gene025115 | PPI |
| MS.gene025114 | MS.gene026084 | PPI |
| MS.gene84671 | MS.gene025114 | PPI |
| MS.gene025114 | MS.gene026083 | PPI |
| MS.gene025114 | MS.gene025118 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025114.t1 | MTR_2g039510 | 79.853 | 273 | 53 | 2 | 213 | 483 | 1 | 273 | 3.19e-155 | 442 |
| MS.gene025114.t1 | MTR_2g039510 | 65.992 | 247 | 46 | 2 | 1 | 209 | 1 | 247 | 5.19e-109 | 324 |
| MS.gene025114.t1 | MTR_2g039480 | 78.195 | 266 | 56 | 2 | 220 | 483 | 4 | 269 | 6.85e-147 | 421 |
| MS.gene025114.t1 | MTR_2g039480 | 63.710 | 248 | 52 | 2 | 8 | 217 | 4 | 251 | 1.27e-102 | 308 |
| MS.gene025114.t1 | MTR_2g039450 | 72.862 | 269 | 71 | 2 | 213 | 480 | 1 | 268 | 7.73e-136 | 394 |
| MS.gene025114.t1 | MTR_2g039450 | 73.061 | 245 | 28 | 3 | 1 | 208 | 1 | 244 | 1.53e-117 | 347 |
| MS.gene025114.t1 | MTR_2g039570 | 73.664 | 262 | 67 | 2 | 223 | 483 | 5 | 265 | 3.53e-135 | 391 |
| MS.gene025114.t1 | MTR_2g039570 | 62.821 | 234 | 49 | 2 | 13 | 209 | 7 | 239 | 1.36e-96 | 292 |
| MS.gene025114.t1 | MTR_4g126050 | 65.744 | 289 | 95 | 4 | 193 | 481 | 38 | 322 | 1.96e-128 | 376 |
| MS.gene025114.t1 | MTR_4g126050 | 53.360 | 253 | 79 | 3 | 1 | 217 | 57 | 306 | 2.78e-82 | 258 |
| MS.gene025114.t1 | MTR_4g126120 | 63.197 | 269 | 95 | 3 | 213 | 480 | 1 | 266 | 4.18e-119 | 350 |
| MS.gene025114.t1 | MTR_4g126120 | 51.575 | 254 | 83 | 4 | 1 | 217 | 1 | 251 | 7.97e-83 | 257 |
| MS.gene025114.t1 | MTR_4g126120 | 63.566 | 258 | 91 | 2 | 224 | 480 | 20 | 275 | 5.39e-115 | 340 |
| MS.gene025114.t1 | MTR_4g126120 | 51.852 | 243 | 78 | 3 | 12 | 217 | 20 | 260 | 1.33e-79 | 249 |
| MS.gene025114.t1 | MTR_1g016050 | 57.430 | 249 | 103 | 2 | 225 | 472 | 7 | 253 | 5.35e-93 | 284 |
| MS.gene025114.t1 | MTR_1g016050 | 47.863 | 234 | 83 | 3 | 13 | 209 | 7 | 238 | 9.65e-65 | 211 |
| MS.gene025114.t1 | MTR_4g126050 | 66.010 | 203 | 65 | 4 | 193 | 395 | 38 | 236 | 1.40e-85 | 263 |
| MS.gene025114.t1 | MTR_4g126050 | 64.901 | 151 | 50 | 2 | 1 | 151 | 57 | 204 | 2.41e-61 | 201 |
| MS.gene025114.t1 | MTR_1g016060 | 44.509 | 173 | 76 | 2 | 301 | 472 | 466 | 619 | 3.11e-31 | 127 |
| MS.gene025114.t1 | MTR_1g016060 | 39.552 | 134 | 63 | 2 | 89 | 204 | 466 | 599 | 8.94e-19 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025114.t1 | AT3G47590 | 62.400 | 250 | 91 | 2 | 226 | 475 | 59 | 305 | 4.52e-105 | 316 |
| MS.gene025114.t1 | AT3G47590 | 49.791 | 239 | 83 | 2 | 14 | 217 | 59 | 295 | 5.03e-74 | 236 |
| MS.gene025114.t1 | AT1G29840 | 57.678 | 267 | 109 | 3 | 209 | 475 | 46 | 308 | 6.49e-102 | 308 |
| MS.gene025114.t1 | AT1G29840 | 48.347 | 242 | 88 | 2 | 4 | 210 | 52 | 291 | 2.69e-73 | 234 |
| MS.gene025114.t1 | AT3G47560 | 61.290 | 248 | 93 | 2 | 225 | 472 | 9 | 253 | 1.66e-99 | 300 |
| MS.gene025114.t1 | AT3G47560 | 48.536 | 239 | 86 | 2 | 13 | 216 | 9 | 245 | 4.31e-72 | 229 |
| MS.gene025114.t1 | AT3G47560 | 61.290 | 248 | 93 | 2 | 225 | 472 | 45 | 289 | 2.23e-99 | 301 |
| MS.gene025114.t1 | AT3G47560 | 48.536 | 239 | 86 | 2 | 13 | 216 | 45 | 281 | 1.29e-71 | 229 |
| MS.gene025114.t1 | AT5G11910 | 60.000 | 250 | 97 | 2 | 224 | 472 | 38 | 285 | 5.11e-99 | 300 |
| MS.gene025114.t1 | AT5G11910 | 47.737 | 243 | 88 | 2 | 12 | 217 | 38 | 278 | 8.34e-67 | 218 |
| MS.gene025114.t1 | AT5G11910 | 60.000 | 250 | 97 | 2 | 224 | 472 | 20 | 267 | 6.55e-99 | 300 |
| MS.gene025114.t1 | AT5G11910 | 47.737 | 243 | 88 | 2 | 12 | 217 | 20 | 260 | 6.65e-67 | 217 |
| MS.gene025114.t1 | AT3G47560 | 55.678 | 273 | 94 | 2 | 225 | 472 | 9 | 279 | 1.27e-95 | 291 |
| MS.gene025114.t1 | AT3G47560 | 42.642 | 265 | 89 | 4 | 13 | 216 | 9 | 271 | 2.41e-60 | 200 |
| MS.gene025114.t1 | AT3G47560 | 55.273 | 275 | 93 | 3 | 225 | 472 | 9 | 280 | 3.29e-93 | 285 |
| MS.gene025114.t1 | AT3G47560 | 43.609 | 266 | 86 | 3 | 13 | 216 | 9 | 272 | 1.70e-65 | 213 |
| MS.gene025114.t1 | AT3G47560 | 50.667 | 300 | 94 | 3 | 225 | 472 | 9 | 306 | 2.79e-89 | 276 |
| MS.gene025114.t1 | AT3G47560 | 38.699 | 292 | 89 | 5 | 13 | 216 | 9 | 298 | 5.73e-54 | 184 |
| MS.gene025114.t1 | AT2G19550 | 52.756 | 254 | 110 | 6 | 227 | 475 | 5 | 253 | 8.91e-83 | 259 |
| MS.gene025114.t1 | AT2G19550 | 46.538 | 260 | 93 | 7 | 15 | 230 | 5 | 262 | 1.17e-64 | 212 |
| MS.gene025114.t1 | AT3G47590 | 65.605 | 157 | 52 | 1 | 226 | 382 | 59 | 213 | 5.34e-66 | 212 |
| MS.gene025114.t1 | AT3G47590 | 52.023 | 173 | 76 | 4 | 14 | 181 | 59 | 229 | 1.76e-50 | 172 |
| MS.gene025114.t1 | AT5G11910 | 60.839 | 143 | 54 | 1 | 224 | 366 | 38 | 178 | 3.80e-54 | 180 |
| MS.gene025114.t1 | AT5G11910 | 58.571 | 140 | 56 | 1 | 12 | 151 | 38 | 175 | 4.82e-51 | 172 |
| MS.gene025114.t1 | AT5G11910 | 60.839 | 143 | 54 | 1 | 224 | 366 | 20 | 160 | 5.47e-54 | 179 |
| MS.gene025114.t1 | AT5G11910 | 58.571 | 140 | 56 | 1 | 12 | 151 | 20 | 157 | 4.01e-51 | 171 |
Find 68 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATCTTGGTAAAGATTATT+TGG | 0.156761 | 2.4:+50461530 | MS.gene025114:CDS |
| AAGATTATTTGGAAAGAATT+AGG | 0.246216 | 2.4:+50461541 | MS.gene025114:CDS |
| CGTATACGAGGGATAACTTT+TGG | 0.260486 | 2.4:+50456079 | MS.gene025114:CDS |
| CTTGAACCTTGCTGTTGAAT+TGG | 0.268661 | 2.4:+50439953 | MS.gene025114:CDS |
| ACACAAGATAACAATTTCTT+TGG | 0.270855 | 2.4:-50439782 | None:intergenic |
| GGATTACACGATTTGCTTCA+TGG | 0.317005 | 2.4:-50461125 | None:intergenic |
| GGCGTATTACATGAAACTTC+TGG | 0.335418 | 2.4:+50459911 | MS.gene025114:CDS |
| AGTAACCTTGTTGAGGTTCA+TGG | 0.338262 | 2.4:-50462064 | None:intergenic |
| GGCAGAGTCCTTACGGTTCA+TGG | 0.347723 | 2.4:+50457233 | MS.gene025114:intron |
| CGTGTAATCCGTGCAATTGT+TGG | 0.348094 | 2.4:+50461138 | MS.gene025114:CDS |
| CGTTTCAGAACGGTAACTAT+TGG | 0.372214 | 2.4:+50461064 | MS.gene025114:CDS |
| CGTGTTATCCGTGCAATTGT+TGG | 0.375403 | 2.4:+50456153 | MS.gene025114:CDS |
| AAAACTGTTGTCAACGTCTC+TGG | 0.396107 | 2.4:+50456681 | MS.gene025114:CDS |
| TTCAGATTGACAAAGATTGC+AGG | 0.418708 | 2.4:+50456845 | MS.gene025114:CDS |
| AAACTTACCAATGCCTTCAA+TGG | 0.437714 | 2.4:-50456724 | None:intergenic |
| TTCATGGTTCTTGCGACGAA+TGG | 0.443994 | 2.4:+50457249 | MS.gene025114:CDS |
| CACAAGTTGCATGTCATTGA+AGG | 0.457279 | 2.4:+50461973 | MS.gene025114:CDS |
| AAAACTGTTGTCAACCTCTC+TGG | 0.459812 | 2.4:+50461477 | MS.gene025114:CDS |
| GGCAGGGTCCTTACAGTTCA+CGG | 0.470147 | 2.4:+50461889 | MS.gene025114:intron |
| AGCAAGGTTCACTATGAGAT+TGG | 0.470630 | 2.4:-50460082 | None:intergenic |
| CATATATGGCAGAGTCCTTA+CGG | 0.473613 | 2.4:+50457226 | MS.gene025114:intron |
| CACAAGCTGCATATCATTGA+AGG | 0.473648 | 2.4:+50457317 | MS.gene025114:CDS |
| TTCATTGATGTGAAGAGGCC+AGG | 0.473908 | 2.4:+50461573 | MS.gene025114:CDS |
| ATGATATGCAGCTTGTGGTT+TGG | 0.478982 | 2.4:-50457311 | None:intergenic |
| GATCTGAAGAGAACCATTGA+AGG | 0.480706 | 2.4:+50456711 | MS.gene025114:CDS |
| TTTGAAGCTTCAGTGAATTG+AGG | 0.484071 | 2.4:+50459722 | MS.gene025114:intron |
| CTTCAATGATATGCAGCTTG+TGG | 0.487163 | 2.4:-50457316 | None:intergenic |
| TCAAAATTTCTTCAGTAAGA+CGG | 0.490951 | 2.4:-50461720 | None:intergenic |
| GGATAACACGATTTGATTCA+CGG | 0.494013 | 2.4:-50456140 | None:intergenic |
| GACATTGTTATCTTGTGTCA+TGG | 0.497964 | 2.4:+50459944 | MS.gene025114:CDS |
| CTTCAATGACATGCAACTTG+TGG | 0.505270 | 2.4:-50461972 | None:intergenic |
| GAAATTGTTATCTTGTGTCA+TGG | 0.508242 | 2.4:+50439787 | MS.gene025114:CDS |
| TACTGTGTCCAACAATTGCA+CGG | 0.513231 | 2.4:-50461146 | None:intergenic |
| TGCATTCTCTGTCAATATGA+AGG | 0.516067 | 2.4:-50461777 | None:intergenic |
| TTGGAAAGAATTAGGAAGGA+CGG | 0.522675 | 2.4:+50461549 | MS.gene025114:CDS |
| AAGAGAACCATTGAAGGCAT+TGG | 0.523690 | 2.4:+50456717 | MS.gene025114:CDS |
| CGGAAGAACCGTGAACTGTA+AGG | 0.528085 | 2.4:-50461897 | None:intergenic |
| TTATTTGGAAAGAATTAGGA+AGG | 0.529949 | 2.4:+50461545 | MS.gene025114:CDS |
| AGTTGTTGTCAGCTTCATCA+AGG | 0.532584 | 2.4:+50462038 | MS.gene025114:CDS |
| TACTATGTCCAACAATTGCA+CGG | 0.534056 | 2.4:-50456161 | None:intergenic |
| CCTTGCTGATGCATTGGAAA+AGG | 0.541626 | 2.4:+50460098 | MS.gene025114:CDS |
| AGTGAACCTTGCTGATGCAT+TGG | 0.541684 | 2.4:+50460092 | MS.gene025114:CDS |
| GCAATTGTTGGACATAGTAA+AGG | 0.544207 | 2.4:+50456165 | MS.gene025114:CDS |
| CACATCCAGAAAAGTCAAAA+CGG | 0.548962 | 2.4:-50453630 | None:intergenic |
| GCAATTGTTGGACACAGTAA+AGG | 0.550180 | 2.4:+50461150 | MS.gene025114:CDS |
| CTCTGGACGTTATGATCTGA+AGG | 0.550517 | 2.4:+50461494 | MS.gene025114:CDS |
| TGCAATCTTTGTCAATCTGA+AGG | 0.554266 | 2.4:-50456843 | None:intergenic |
| GCAGGCATCGAAGAACATCT+TGG | 0.554713 | 2.4:+50461516 | MS.gene025114:CDS |
| TTGAACGGAGTTAATTTCAT+CGG | 0.556866 | 2.4:-50461917 | None:intergenic |
| CATTTCCTGAAAAGTCAAAA+CGG | 0.561669 | 2.4:-50460137 | None:intergenic |
| ACGGTTTCATTGATGTGAAG+AGG | 0.562563 | 2.4:+50461568 | MS.gene025114:CDS |
| GGACGTTATGATCTGAAGGC+AGG | 0.563099 | 2.4:+50461498 | MS.gene025114:CDS |
| TTCATATTGACAGAGAATGC+AGG | 0.592777 | 2.4:+50461779 | MS.gene025114:CDS |
| AGCTAGCAGTAACCTTGTTG+AGG | 0.594917 | 2.4:-50462071 | None:intergenic |
| ATCATAATACACAACAAAAG+CGG | 0.609993 | 2.4:+50439718 | MS.gene025114:CDS |
| CCTTGCTGTTGAATTGGAGA+AGG | 0.613268 | 2.4:+50439959 | MS.gene025114:CDS |
| CCTTCTCCAATTCAACAGCA+AGG | 0.620394 | 2.4:-50439959 | None:intergenic |
| CGCAAGAACCATGAACCGTA+AGG | 0.629336 | 2.4:-50457241 | None:intergenic |
| AAAGCGAAGGTACGTATACG+AGG | 0.632633 | 2.4:+50456067 | MS.gene025114:CDS |
| CGGTAACTATTGGACAGAGG+TGG | 0.646830 | 2.4:+50461074 | MS.gene025114:CDS |
| AGTGAAGGTTCGTTTCAGAA+CGG | 0.652849 | 2.4:+50461054 | MS.gene025114:CDS |
| TCCCTAGCTTCACAAAACCC+AGG | 0.663130 | 2.4:+50439590 | MS.gene025114:CDS |
| GGAAACCATGAACCTCAACA+AGG | 0.682330 | 2.4:+50462059 | MS.gene025114:CDS |
| AAACGCGTATGCATCTTGAA+CGG | 0.689910 | 2.4:-50461932 | None:intergenic |
| GATGAAGCTGACAACAACTG+AGG | 0.711495 | 2.4:-50462034 | None:intergenic |
| TCAGATCATAACGTCCAGAG+AGG | 0.713623 | 2.4:-50461491 | None:intergenic |
| AAGCGAAGGTACGTATACGA+GGG | 0.720170 | 2.4:+50456068 | MS.gene025114:CDS |
| GAACGGTAACTATTGGACAG+AGG | 0.732928 | 2.4:+50461071 | MS.gene025114:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 50439587 | 50462095 | 50439587 | ID=MS.gene025114 |
| chr2.4 | mRNA | 50439587 | 50462095 | 50439587 | ID=MS.gene025114.t1;Parent=MS.gene025114 |
| chr2.4 | exon | 50439587 | 50439611 | 50439587 | ID=MS.gene025114.t1.exon1;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50439587 | 50439611 | 50439587 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50439698 | 50439825 | 50439698 | ID=MS.gene025114.t1.exon2;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50439698 | 50439825 | 50439698 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50439939 | 50439980 | 50439939 | ID=MS.gene025114.t1.exon3;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50439939 | 50439980 | 50439939 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50453613 | 50453653 | 50453613 | ID=MS.gene025114.t1.exon4;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50453613 | 50453653 | 50453613 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50456065 | 50456186 | 50456065 | ID=MS.gene025114.t1.exon5;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50456065 | 50456186 | 50456065 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50456637 | 50456738 | 50456637 | ID=MS.gene025114.t1.exon6;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50456637 | 50456738 | 50456637 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50456821 | 50456866 | 50456821 | ID=MS.gene025114.t1.exon7;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50456821 | 50456866 | 50456821 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50457238 | 50457351 | 50457238 | ID=MS.gene025114.t1.exon8;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50457238 | 50457351 | 50457238 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50459729 | 50459769 | 50459729 | ID=MS.gene025114.t1.exon9;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50459729 | 50459769 | 50459729 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50459855 | 50459982 | 50459855 | ID=MS.gene025114.t1.exon10;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50459855 | 50459982 | 50459855 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50460078 | 50460160 | 50460078 | ID=MS.gene025114.t1.exon11;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50460078 | 50460160 | 50460078 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50461050 | 50461171 | 50461050 | ID=MS.gene025114.t1.exon12;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50461050 | 50461171 | 50461050 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50461433 | 50461594 | 50461433 | ID=MS.gene025114.t1.exon13;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50461433 | 50461594 | 50461433 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50461707 | 50461800 | 50461707 | ID=MS.gene025114.t1.exon14;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50461707 | 50461800 | 50461707 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| chr2.4 | exon | 50461894 | 50462095 | 50461894 | ID=MS.gene025114.t1.exon15;Parent=MS.gene025114.t1 |
| chr2.4 | CDS | 50461894 | 50462095 | 50461894 | ID=cds.MS.gene025114.t1;Parent=MS.gene025114.t1 |
| Gene Sequence |
| Protein sequence |
