AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025115

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025115.t1 MTR_2g039510 78.388 273 10 3 11 234 1 273 3.29e-148 414
MS.gene025115.t1 MTR_2g039480 68.421 266 35 3 18 234 4 269 7.22e-124 352
MS.gene025115.t1 MTR_2g039570 65.900 261 42 1 21 234 5 265 4.91e-119 340
MS.gene025115.t1 MTR_2g039450 64.419 267 48 1 11 230 1 267 1.46e-116 335
MS.gene025115.t1 MTR_4g126050 58.955 268 62 3 11 232 57 322 3.09e-106 310
MS.gene025115.t1 MTR_4g126120 56.489 262 65 3 11 225 1 260 1.16e-100 293
MS.gene025115.t1 MTR_4g126120 57.028 249 59 2 24 225 22 269 5.07e-97 284
MS.gene025115.t1 MTR_4g126050 69.399 183 54 2 11 193 57 237 5.26e-89 263
MS.gene025115.t1 MTR_1g016050 51.012 247 73 2 24 223 8 253 3.39e-80 242
MS.gene025115.t1 MTR_1g016060 40.244 164 70 2 98 233 466 629 1.08e-26 108
MS.gene025115.t1 MTR_2g039550 44.048 84 22 2 6 88 11 70 1.67e-12 61.2
MS.gene025115.t1 MTR_4g124530 52.830 53 22 1 129 178 14 66 3.37e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025115.t1 AT3G47590 54.582 251 68 3 24 229 59 308 4.42e-89 265
MS.gene025115.t1 AT1G29840 51.481 270 85 2 5 229 43 311 1.00e-86 259
MS.gene025115.t1 AT3G47560 52.653 245 70 2 24 223 10 253 1.01e-83 250
MS.gene025115.t1 AT3G47560 50.965 259 79 3 10 223 34 289 1.81e-83 251
MS.gene025115.t1 AT5G11910 49.020 255 81 4 16 223 33 285 1.76e-79 241
MS.gene025115.t1 AT5G11910 49.398 249 78 3 22 223 20 267 2.19e-79 240
MS.gene025115.t1 AT3G47560 47.426 272 70 3 24 223 10 280 2.33e-77 235
MS.gene025115.t1 AT3G47590 69.231 156 47 1 24 179 59 213 2.25e-76 230
MS.gene025115.t1 AT3G47560 66.242 157 52 1 24 180 10 165 4.60e-71 219
MS.gene025115.t1 AT2G19550 48.016 252 78 6 25 226 5 253 1.00e-69 217
MS.gene025115.t1 AT3G47560 56.522 184 52 2 24 180 10 192 8.06e-65 204
MS.gene025115.t1 AT5G11910 60.811 148 56 2 16 163 33 178 2.60e-61 190
MS.gene025115.t1 AT5G11910 60.811 148 56 2 16 163 15 160 3.57e-61 189

Find 33 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATTATTTGAAAAGAATT+AGG 0.263962 2.4:+50437391 MS.gene025115:CDS
ATGATATGTAACTTGTGATT+TGG 0.372549 2.4:-50438076 None:intergenic
GTTGGCATATTGCATGAAAC+TGG 0.379165 2.4:+50435406 MS.gene025115:CDS
AGTACTCTTGTTGTGGTCAA+TGG 0.384576 2.4:-50438173 None:intergenic
TTTGATGTGAAGAGGAGTTC+AGG 0.389844 2.4:+50437426 MS.gene025115:CDS
AAAACTGTTGTTAACCTCTC+TGG 0.430009 2.4:+50437327 MS.gene025115:CDS
ATTGAACCTTGCTGCTGCAT+TGG 0.440960 2.4:+50435591 MS.gene025115:CDS
CGTGTGATCCGTGCAATTGT+TGG 0.442805 2.4:+50436716 MS.gene025115:CDS
CAACAAGAGTACTGCTAGCT+AGG 0.447007 2.4:+50438183 MS.gene025115:CDS
CCTTGCTGCTGCATTGGAAA+AGG 0.467817 2.4:+50435597 MS.gene025115:CDS
CGTTTGAGTACGGTAACTAC+TGG 0.470793 2.4:+50436642 MS.gene025115:CDS
CACAAGTTACATATCATTGA+AGG 0.476698 2.4:+50438082 MS.gene025115:CDS
GACATTGTTATCTTATGTCA+TGG 0.505179 2.4:+50435439 MS.gene025115:CDS
GGATCACACGATTTGATTCA+CGG 0.506479 2.4:-50436703 None:intergenic
TTGAAAAGAATTAGGAAGGA+CGG 0.509594 2.4:+50437399 MS.gene025115:CDS
TTATTTGAAAAGAATTAGGA+AGG 0.529949 2.4:+50437395 MS.gene025115:CDS
ACGGTTTCTTTGATGTGAAG+AGG 0.543472 2.4:+50437418 MS.gene025115:CDS
GCAATTGTTGGACATAGTAA+AGG 0.544207 2.4:+50436728 MS.gene025115:CDS
AGTTCTTACGAGCTTCATCA+AGG 0.545473 2.4:+50438147 MS.gene025115:CDS
TACTATGTCCAACAATTGCA+CGG 0.547440 2.4:-50436724 None:intergenic
CTCTGGACGTTATGATCTGA+AGG 0.550517 2.4:+50437344 MS.gene025115:CDS
AGCGAAGGTTCGTTTGAGTA+CGG 0.557534 2.4:+50436632 MS.gene025115:CDS
GGACGTTATGATCTGAAGGC+AGG 0.563099 2.4:+50437348 MS.gene025115:CDS
AGCTAGCAGTACTCTTGTTG+TGG 0.580225 2.4:-50438180 None:intergenic
CATTTCCAGAAAAGTCAAAA+CGG 0.584654 2.4:-50435636 None:intergenic
CGGTAACTACTGGAAAGAAG+TGG 0.591735 2.4:+50436652 MS.gene025115:CDS
GCAGGCATTGAAGAACGCCT+TGG 0.597706 2.4:+50437366 MS.gene025115:CDS
GATATTTGGAAGCATACAGA+AGG 0.625385 2.4:-50437296 None:intergenic
ATGACATAAGATAACAATGT+CGG 0.639340 2.4:-50435437 None:intergenic
TTTCCTTCAAGGGAAAGCGA+AGG 0.664955 2.4:+50436617 MS.gene025115:intron
ACATAAGATAACAATGTCGG+TGG 0.673820 2.4:-50435434 None:intergenic
GATGAAGCTCGTAAGAACTG+AGG 0.686004 2.4:-50438143 None:intergenic
TCAGATCATAACGTCCAGAG+AGG 0.713623 2.4:-50437341 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAGTTTTTAAAAAAAGTA+TGG - chr2.4:50437972-50437991 None:intergenic 10.0%
!!! TTTTTTTCTCTTTATATTAT+AGG + chr2.4:50435555-50435574 MS.gene025115:intron 10.0%
!! AAGATTATTTGAAAAGAATT+AGG + chr2.4:50437391-50437410 MS.gene025115:CDS 15.0%
!! ATTCATTAAATGATCATTTA+AGG + chr2.4:50437652-50437671 MS.gene025115:intron 15.0%
!! GTAATAATTTGAGAAAAAAA+TGG - chr2.4:50436075-50436094 None:intergenic 15.0%
!! AAAAAGAAAAAGAAACAAGA+AGG - chr2.4:50435494-50435513 None:intergenic 20.0%
!! AAAATATAAGTCAATCCAAA+CGG + chr2.4:50435757-50435776 MS.gene025115:intron 20.0%
!! AATACCATGATAAAATGATA+AGG - chr2.4:50436460-50436479 None:intergenic 20.0%
!! ACATAAATTTATTGTACATG+AGG - chr2.4:50436520-50436539 None:intergenic 20.0%
!! ATCTCTATAATGATATTTGT+TGG + chr2.4:50437157-50437176 MS.gene025115:intron 20.0%
!! CAATGAATGATAGTATAAAT+TGG - chr2.4:50436838-50436857 None:intergenic 20.0%
!! GATAAAATGATAAGGAAATT+GGG - chr2.4:50436452-50436471 None:intergenic 20.0%
!! TACATAAGAATTTGAGAAAA+TGG - chr2.4:50437200-50437219 None:intergenic 20.0%
!! TGATAAAATGATAAGGAAAT+TGG - chr2.4:50436453-50436472 None:intergenic 20.0%
!! TTATTTGAAAAGAATTAGGA+AGG + chr2.4:50437395-50437414 MS.gene025115:CDS 20.0%
!!! ATACCTATTTGTTAAAATGA+AGG - chr2.4:50437130-50437149 None:intergenic 20.0%
!!! ATTTCCTTATCATTTTATCA+TGG + chr2.4:50436453-50436472 MS.gene025115:intron 20.0%
!!! TATCCTTCATTTTAACAAAT+AGG + chr2.4:50437124-50437143 MS.gene025115:intron 20.0%
!!! TCAATTTTCATTATCCATAA+AGG - chr2.4:50437577-50437596 None:intergenic 20.0%
!!! TTAGAACTTTAGAATTCAAA+TGG + chr2.4:50437547-50437566 MS.gene025115:intron 20.0%
! AAAAATGGTGAAAAACTTGT+TGG + chr2.4:50435388-50435407 MS.gene025115:CDS 25.0%
! AAATATAAGTCAATCCAAAC+GGG + chr2.4:50435758-50435777 MS.gene025115:intron 25.0%
! AACATGAAGATGTTAAGTTA+TGG + chr2.4:50436789-50436808 MS.gene025115:intron 25.0%
! ATCATAATACCCAACAAAAA+TGG + chr2.4:50435373-50435392 MS.gene025115:CDS 25.0%
! ATGACATAAGATAACAATGT+CGG - chr2.4:50435440-50435459 None:intergenic 25.0%
! GATTTATACTGCAAAATGAT+AGG + chr2.4:50435882-50435901 MS.gene025115:intron 25.0%
! GGTATACTTCTTATGATTTA+GGG + chr2.4:50435659-50435678 MS.gene025115:intron 25.0%
! TACAGCAATTAATCATCTTT+CGG - chr2.4:50436181-50436200 None:intergenic 25.0%
! TATATCCAAACTCAAAACAT+AGG - chr2.4:50436217-50436236 None:intergenic 25.0%
! TTGAATGTAAGAAACTAACA+TGG - chr2.4:50435270-50435289 None:intergenic 25.0%
!! AAGATGTTAAGTTATGGTTT+TGG + chr2.4:50436795-50436814 MS.gene025115:intron 25.0%
!! ACAATTCCTTTTCCAAAATT+GGG + chr2.4:50436485-50436504 MS.gene025115:intron 25.0%
!! ACATGAGCATTGTATTAATT+TGG + chr2.4:50437628-50437647 MS.gene025115:intron 25.0%
!! ATGATATGTAACTTGTGATT+TGG - chr2.4:50438079-50438098 None:intergenic 25.0%
!! ATTCAAATGGATTTCCTTTA+TGG + chr2.4:50437560-50437579 MS.gene025115:intron 25.0%
!! TTTTCAAATAATCTTTGCCA+AGG - chr2.4:50437386-50437405 None:intergenic 25.0%
!!! AAGTTTTTCACCATTTTTGT+TGG - chr2.4:50435386-50435405 None:intergenic 25.0%
!!! AGTTTTAATGTCGTGATATT+TGG - chr2.4:50437313-50437332 None:intergenic 25.0%
!!! AGTTTTTCACCATTTTTGTT+GGG - chr2.4:50435385-50435404 None:intergenic 25.0%
!!! GGTTTTTAGTTTAGGATTAT+TGG + chr2.4:50436392-50436411 MS.gene025115:intron 25.0%
!!! TATGTTTTGAGTTTGGATAT+AGG + chr2.4:50436216-50436235 MS.gene025115:intron 25.0%
!!! TTGAACTTATGCTTTTTAGT+GGG + chr2.4:50436133-50436152 MS.gene025115:intron 25.0%
!!! TTTGAACTTATGCTTTTTAG+TGG + chr2.4:50436132-50436151 MS.gene025115:intron 25.0%
CAATCTTAACATGCAAAGTA+TGG - chr2.4:50436923-50436942 None:intergenic 30.0%
CACAAGTTACATATCATTGA+AGG + chr2.4:50438082-50438101 MS.gene025115:CDS 30.0%
CATTTCCAGAAAAGTCAAAA+CGG - chr2.4:50435639-50435658 None:intergenic 30.0%
GACATTGTTATCTTATGTCA+TGG + chr2.4:50435439-50435458 MS.gene025115:CDS 30.0%
GATACTTCTTGTCATAATTG+AGG - chr2.4:50436879-50436898 None:intergenic 30.0%
GATGAATGAACTGAATCATA+TGG + chr2.4:50436251-50436270 MS.gene025115:intron 30.0%
GGGTATACTTCTTATGATTT+AGG + chr2.4:50435658-50435677 MS.gene025115:intron 30.0%
GTCATAATTGAGGTGTATAA+TGG - chr2.4:50436869-50436888 None:intergenic 30.0%
TGTTATTACTCTTACAGTTC+TGG + chr2.4:50436371-50436390 MS.gene025115:intron 30.0%
TTGAAAAGAATTAGGAAGGA+CGG + chr2.4:50437399-50437418 MS.gene025115:CDS 30.0%
! AACATGGATTTTGTGAAGAT+AGG - chr2.4:50435254-50435273 None:intergenic 30.0%
! ACATGGATTTTGTGAAGATA+GGG - chr2.4:50435253-50435272 None:intergenic 30.0%
! AGGAAAATACTACCCAATTT+TGG - chr2.4:50436500-50436519 None:intergenic 30.0%
! GACAATTCCTTTTCCAAAAT+TGG + chr2.4:50436484-50436503 MS.gene025115:intron 30.0%
! TGGACATAGTAAAGGTATAT+TGG + chr2.4:50436736-50436755 MS.gene025115:intron 30.0%
!! ATACTACCCAATTTTGGAAA+AGG - chr2.4:50436494-50436513 None:intergenic 30.0%
!! GATGAAGTTCAATTTTAGCA+TGG - chr2.4:50437102-50437121 None:intergenic 30.0%
!! TGAACTTATGCTTTTTAGTG+GGG + chr2.4:50436134-50436153 MS.gene025115:intron 30.0%
!! TTCTCTTAACTTTAGCTTGT+GGG + chr2.4:50436593-50436612 MS.gene025115:intron 30.0%
!! TTGGAAGGGTGTATATTTTT+AGG + chr2.4:50435939-50435958 MS.gene025115:intron 30.0%
!! TTTCTCTTAACTTTAGCTTG+TGG + chr2.4:50436592-50436611 MS.gene025115:intron 30.0%
!!! ACAGTTCTGGTTTTTAGTTT+AGG + chr2.4:50436384-50436403 MS.gene025115:intron 30.0%
!!! ATTTGCCTATGTTTTGAGTT+TGG + chr2.4:50436209-50436228 MS.gene025115:intron 30.0%
!!! GTTTTGACTTTTCTGGAAAT+GGG + chr2.4:50435638-50435657 MS.gene025115:CDS 30.0%
AAAACTGTTGTTAACCTCTC+TGG + chr2.4:50437327-50437346 MS.gene025115:CDS 35.0%
AAAAGGAGAAGAGATCAAAC+AGG - chr2.4:50437800-50437819 None:intergenic 35.0%
ACATAAGATAACAATGTCGG+TGG - chr2.4:50435437-50435456 None:intergenic 35.0%
ACATACTGTTGCAGGAAATT+TGG + chr2.4:50437677-50437696 MS.gene025115:intron 35.0%
AGAAAGCAAAGAATGAAGCA+AGG - chr2.4:50436577-50436596 None:intergenic 35.0%
AGGATTGATGTGATTTCATG+TGG + chr2.4:50435959-50435978 MS.gene025115:intron 35.0%
ATGTGCATCTTGTAACAAAC+AGG - chr2.4:50438038-50438057 None:intergenic 35.0%
CATGCTTGAACAACAAAGAA+TGG - chr2.4:50437833-50437852 None:intergenic 35.0%
GATAAATGAAAGCTCATTCC+TGG - chr2.4:50437003-50437022 None:intergenic 35.0%
GCAATTGTTGGACATAGTAA+AGG + chr2.4:50436728-50436747 MS.gene025115:CDS 35.0%
GTAGACTATAAAGAACACAC+TGG - chr2.4:50436109-50436128 None:intergenic 35.0%
TACTATGTCCAACAATTGCA+CGG - chr2.4:50436727-50436746 None:intergenic 35.0%
TCAAACTTTCCTCAGTAACA+CGG - chr2.4:50437707-50437726 None:intergenic 35.0%
TGACAAAGATTGCAGGTTAA+TGG + chr2.4:50437770-50437789 MS.gene025115:intron 35.0%
TGTTACTGAGGAAAGTTTGA+TGG + chr2.4:50437707-50437726 MS.gene025115:intron 35.0%
TTCAGGTAAATCGTTTACTC+AGG + chr2.4:50437443-50437462 MS.gene025115:intron 35.0%
! GATATTTGGAAGCATACAGA+AGG - chr2.4:50437299-50437318 None:intergenic 35.0%
!!! CGTTTTGACTTTTCTGGAAA+TGG + chr2.4:50435637-50435656 MS.gene025115:CDS 35.0%
!!! TCTTTCCGTTTTGACTTTTC+TGG + chr2.4:50435631-50435650 MS.gene025115:CDS 35.0%
ACCAAAGCAAACACATCAGA+CGG - chr2.4:50436308-50436327 None:intergenic 40.0%
ACGGTTTCTTTGATGTGAAG+AGG + chr2.4:50437418-50437437 MS.gene025115:CDS 40.0%
AGAATGGAGCTAGCAGTAAA+AGG - chr2.4:50437817-50437836 None:intergenic 40.0%
AGTTCTTACGAGCTTCATCA+AGG + chr2.4:50438147-50438166 MS.gene025115:CDS 40.0%
ATAAGGAAATTGGGTCACTG+AGG - chr2.4:50436443-50436462 None:intergenic 40.0%
CCATCATCCTAGCAAACTTT+GGG - chr2.4:50437229-50437248 None:intergenic 40.0%
GAAGAGATTTGCGTAACTTC+TGG + chr2.4:50436964-50436983 MS.gene025115:intron 40.0%
GCATCTTGTAACAAACAGGT+AGG - chr2.4:50438034-50438053 None:intergenic 40.0%
GGATCACACGATTTGATTCA+CGG - chr2.4:50436706-50436725 None:intergenic 40.0%
GTTGGCATATTGCATGAAAC+TGG + chr2.4:50435406-50435425 MS.gene025115:CDS 40.0%
TCCATCATCCTAGCAAACTT+TGG - chr2.4:50437230-50437249 None:intergenic 40.0%
TCTGGCAAAAAACATACACC+AGG + chr2.4:50436982-50437001 MS.gene025115:intron 40.0%
TGCAATCTTTGTCAATCTGC+AGG - chr2.4:50437764-50437783 None:intergenic 40.0%
TGCAGATTGACAAAGATTGC+AGG + chr2.4:50437763-50437782 MS.gene025115:intron 40.0%
TTAAGGACACATACTGTTGC+AGG + chr2.4:50437669-50437688 MS.gene025115:intron 40.0%
TTTGATGTGAAGAGGAGTTC+AGG + chr2.4:50437426-50437445 MS.gene025115:CDS 40.0%
! ACTTTCACCCAAAGTTTGCT+AGG + chr2.4:50437219-50437238 MS.gene025115:intron 40.0%
! AGTACTCTTGTTGTGGTCAA+TGG - chr2.4:50438176-50438195 None:intergenic 40.0%
! TTTGAGTTTGGATATAGGCG+TGG + chr2.4:50436221-50436240 MS.gene025115:intron 40.0%
!! TCCTGGTTGTTCTTTTGGAA+GGG + chr2.4:50435925-50435944 MS.gene025115:intron 40.0%
!! TTGTTTCTGTGTCATGATGC+AGG + chr2.4:50437261-50437280 MS.gene025115:intron 40.0%
!!! GCTTTTTAGTGGGGTATTGA+GGG + chr2.4:50436143-50436162 MS.gene025115:intron 40.0%
!!! TGCTTTTTAGTGGGGTATTG+AGG + chr2.4:50436142-50436161 MS.gene025115:intron 40.0%
AAGAACAACCAGGACAGAGA+TGG - chr2.4:50435919-50435938 None:intergenic 45.0%
ACCCTTCCAAAAGAACAACC+AGG - chr2.4:50435929-50435948 None:intergenic 45.0%
AGCGAAGGTTCGTTTGAGTA+CGG + chr2.4:50436632-50436651 MS.gene025115:CDS 45.0%
AGCTAGCAGTACTCTTGTTG+TGG - chr2.4:50438183-50438202 None:intergenic 45.0%
ATTGAACCTTGCTGCTGCAT+TGG + chr2.4:50435591-50435610 MS.gene025115:CDS 45.0%
CACACTGACATGCCTTCAAT+TGG - chr2.4:50436017-50436036 None:intergenic 45.0%
CATGCCTTCAATTGGAATGC+AGG - chr2.4:50436009-50436028 None:intergenic 45.0%
CCAAAGCAAACACATCAGAC+GGG - chr2.4:50436307-50436326 None:intergenic 45.0%
CGGTAACTACTGGAAAGAAG+TGG + chr2.4:50436652-50436671 MS.gene025115:CDS 45.0%
CGTTTGAGTACGGTAACTAC+TGG + chr2.4:50436642-50436661 MS.gene025115:CDS 45.0%
CTCTGGACGTTATGATCTGA+AGG + chr2.4:50437344-50437363 MS.gene025115:CDS 45.0%
GACACCTGCATTCCAATTGA+AGG + chr2.4:50436002-50436021 MS.gene025115:intron 45.0%
GATGAAGCTCGTAAGAACTG+AGG - chr2.4:50438146-50438165 None:intergenic 45.0%
TCAGATCATAACGTCCAGAG+AGG - chr2.4:50437344-50437363 None:intergenic 45.0%
TTTCCTTCAAGGGAAAGCGA+AGG + chr2.4:50436617-50436636 MS.gene025115:intron 45.0%
TTTCTGTGTCATGATGCAGG+AGG + chr2.4:50437264-50437283 MS.gene025115:intron 45.0%
! AGCTTGTGGGTTTTCCTTCA+AGG + chr2.4:50436606-50436625 MS.gene025115:intron 45.0%
! CTCTGTCCTGGTTGTTCTTT+TGG + chr2.4:50435920-50435939 MS.gene025115:intron 45.0%
! GCTTGTGGGTTTTCCTTCAA+GGG + chr2.4:50436607-50436626 MS.gene025115:intron 45.0%
! GTCCTGGTTGTTCTTTTGGA+AGG + chr2.4:50435924-50435943 MS.gene025115:intron 45.0%
! TTTGGACTACCGTGTTACTG+AGG + chr2.4:50437695-50437714 MS.gene025115:intron 45.0%
!! CCCAAAGTTTGCTAGGATGA+TGG + chr2.4:50437226-50437245 MS.gene025115:intron 45.0%
!!! TCAATTTTTTTAAAATTTTT+TGG - chr2.4:50435299-50435318 None:intergenic 5.0%
AAGCAAACACATCAGACGGG+CGG - chr2.4:50436304-50436323 None:intergenic 50.0%
CCCGTCTGATGTGTTTGCTT+TGG + chr2.4:50436304-50436323 MS.gene025115:intron 50.0%
CCTTGCTGCTGCATTGGAAA+AGG + chr2.4:50435597-50435616 MS.gene025115:CDS 50.0%
CGTGTGATCCGTGCAATTGT+TGG + chr2.4:50436716-50436735 MS.gene025115:CDS 50.0%
GAACACACTGGATACGACAC+TGG - chr2.4:50436097-50436116 None:intergenic 50.0%
GAACCTTCGCTTTCCCTTGA+AGG - chr2.4:50436623-50436642 None:intergenic 50.0%
GCGTTCACTAAAAGCCCGTT+TGG - chr2.4:50435775-50435794 None:intergenic 50.0%
GGACGTTATGATCTGAAGGC+AGG + chr2.4:50437348-50437367 MS.gene025115:CDS 50.0%
GTGACATCGACACACACATC+TGG + chr2.4:50436034-50436053 MS.gene025115:intron 50.0%
TGGATATAGGCGTGGATCAC+TGG + chr2.4:50436229-50436248 MS.gene025115:intron 50.0%
! CCTTTTCCAATGCAGCAGCA+AGG - chr2.4:50435600-50435619 None:intergenic 50.0%
AGACCAAGCCATCTCTGTCC+TGG + chr2.4:50435908-50435927 MS.gene025115:intron 55.0%
CAACCAGGACAGAGATGGCT+TGG - chr2.4:50435914-50435933 None:intergenic 55.0%
!! GCAGGCATTGAAGAACGCCT+TGG + chr2.4:50437366-50437385 MS.gene025115:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 50435216 50438204 50435216 ID=MS.gene025115
chr2.4 mRNA 50435216 50438204 50435216 ID=MS.gene025115.t1;Parent=MS.gene025115
chr2.4 exon 50435216 50435270 50435216 ID=MS.gene025115.t1.exon1;Parent=MS.gene025115.t1
chr2.4 CDS 50435216 50435270 50435216 ID=cds.MS.gene025115.t1;Parent=MS.gene025115.t1
chr2.4 exon 50435353 50435477 50435353 ID=MS.gene025115.t1.exon2;Parent=MS.gene025115.t1
chr2.4 CDS 50435353 50435477 50435353 ID=cds.MS.gene025115.t1;Parent=MS.gene025115.t1
chr2.4 exon 50435577 50435659 50435577 ID=MS.gene025115.t1.exon3;Parent=MS.gene025115.t1
chr2.4 CDS 50435577 50435659 50435577 ID=cds.MS.gene025115.t1;Parent=MS.gene025115.t1
chr2.4 exon 50436628 50436749 50436628 ID=MS.gene025115.t1.exon4;Parent=MS.gene025115.t1
chr2.4 CDS 50436628 50436749 50436628 ID=cds.MS.gene025115.t1;Parent=MS.gene025115.t1
chr2.4 exon 50437283 50437447 50437283 ID=MS.gene025115.t1.exon5;Parent=MS.gene025115.t1
chr2.4 CDS 50437283 50437447 50437283 ID=cds.MS.gene025115.t1;Parent=MS.gene025115.t1
chr2.4 exon 50438050 50438204 50438050 ID=MS.gene025115.t1.exon6;Parent=MS.gene025115.t1
chr2.4 CDS 50438050 50438204 50438050 ID=cds.MS.gene025115.t1;Parent=MS.gene025115.t1
Gene Sequence

>MS.gene025115

ATGCAACTTGAAAACAAGAGTGAAGTGAAGATGTCCCTATCTTCACAAAATCCATGTTAGTTTCTTACATTCAATATAAACCAAAAAATTTTAAAAAAATTGATGAATGATTTATTATTTTTTATTTGTTTTTGTAGCTATTGAGCATTATCAAAGAATCATAATACCCAACAAAAATGGTGAAAAACTTGTTGGCATATTGCATGAAACTGGAACAACCACCGACATTGTTATCTTATGTCATGGTTTTCGTTGCTCTAAAGTTAGTACTTGTTCCTTCTTGTTTCTTTTTCTTTTTTACTTAAATTTATGAATAATTTTTTTTATTAATTTGTGTTTTTTTTTTCTCTTTATATTATAGGATATCAATCTCATATTGAACCTTGCTGCTGCATTGGAAAAGGAACAGATTAGTTCTTTCCGTTTTGACTTTTCTGGAAATGGGTATACTTCTTATGATTTAGGGATTGACTTATTTTTGAACTTATGCAAAACAGCTTATGAAGATACAATTTTCGTTAGATATTTATTTACATAAGCTAAAATATAAGTCAATCCAAACGGGCTTTTAGTGAACGCAATCATATTAACTGAATAATCATTTTGACTACATTTTGATATATAGTCAATCGCATTCTAAATGTTTGTGAAATTGTCTCTAAAATTGATTTATACTGCAAAATGATAGGCTAAGACCAAGCCATCTCTGTCCTGGTTGTTCTTTTGGAAGGGTGTATATTTTTAGGATTGATGTGATTTCATGTGGTTTATGATCTACATAGCACTGACACCTGCATTCCAATTGAAGGCATGTCAGTGTGACATCGACACACACATCTGGTTACATTCAATTATTCCATTTTTTTCTCAAATTATTACCAGTGTCGTATCCAGTGTGTTCTTTATAGTCTACAATTTTGAACTTATGCTTTTTAGTGGGGTATTGAGGGAGTTGATAATAGCCGAAAGATGATTAATTGCTGTACTTAAAGCATTTGCCTATGTTTTGAGTTTGGATATAGGCGTGGATCACTGGATGAATGAACTGAATCATATGGTAGTATGATCACTAAAATAGTGTGTTTCCGCCCGTCTGATGTGTTTGCTTTGGTTTGCTGATGTTATTTATACTTATTTATCATAATTATCTAATGTTGTTATTACTCTTACAGTTCTGGTTTTTAGTTTAGGATTATTGGTTTAGTTGTAGTACTATTGTACTATCCTCAGTGACCCAATTTCCTTATCATTTTATCATGGTATTAGATGACAATTCCTTTTCCAAAATTGGGTAGTATTTTCCTCATGTACAATAAATTTATGTACTAAAGTCTATGCTATCTTGTTTTCATCATTTGCCTTGCTTCATTCTTTGCTTTCTCTTAACTTTAGCTTGTGGGTTTTCCTTCAAGGGAAAGCGAAGGTTCGTTTGAGTACGGTAACTACTGGAAAGAAGTGGATGATTTACATGCTGTTGCTCAACATTTCCGTGAATCAAATCGTGTGATCCGTGCAATTGTTGGACATAGTAAAGGTATATTGGTTTGTTTGCTTATATTGAAGTATTTTAAGCAACATGAAGATGTTAAGTTATGGTTTTGGATATATGATTATGTGCTCCAATTTATACTATCATTCATTGCTAATTTTCCATTATACACCTCAATTATGACAAGAAGTATCGTTATAAAAATATTTTGTCAACCATACTTTGCATGTTAAGATTGCAATGTTGAATGATGCTTTATGAAGAGATTTGCGTAACTTCTGGCAAAAAACATACACCAGGAATGAGCTTTCATTTATCTGATATATAGCTTCTAGCATGAGATTTGTCAATAAGTCGAAAGTTACTATTCTACAAATGAACTTCATTTGCAAAACCATGCTAAAATTGAACTTCATCATTATCCTTCATTTTAACAAATAGGTATTCTATAGATCTCTATAATGATATTTGTTGGTGTAGCTTCTGATCAATCCATTTTCTCAAATTCTTATGTACTTTCACCCAAAGTTTGCTAGGATGATGGAACTGTTAACAGTTGTTTCTGTGTCATGATGCAGGAGGTGATGTAGTCCTTCTGTATGCTTCCAAATATCACGACATTAAAACTGTTGTTAACCTCTCTGGACGTTATGATCTGAAGGCAGGCATTGAAGAACGCCTTGGCAAAGATTATTTGAAAAGAATTAGGAAGGACGGTTTCTTTGATGTGAAGAGGAGTTCAGGTAAATCGTTTACTCAGGCTAAAAATATCACAAGTACAAAGTTCGAGTTAGACATTGATCGTGAACTGTCTTGTTCAATAACTGTAAAATGACTTGAGTTTAGAACTTTAGAATTCAAATGGATTTCCTTTATGGATAATGAAAATTGACAAGTTCTTTGTTTCAGTCTCTATCACTTACACATGAGCATTGTATTAATTTGGTATTCATTAAATGATCATTTAAGGACACATACTGTTGCAGGAAATTTGGACTACCGTGTTACTGAGGAAAGTTTGATGGATCGCTTGAGTACAAATATGCATGAAGCGTGCCTGCAGATTGACAAAGATTGCAGGTTAATGGAAAACCTGTTTGATCTCTTCTCCTTTTACTGCTAGCTCCATTCTTTGTTGTTCAAGCATGCGTCGACGTTGAAATATCACAATAATATTTCTACAATAGAAGCAATAAAAAACATGATATCTCTCAATTCTGATTATTCTTTCATTACAATTACGTGCAATTTAACTACAACATTTCCATACTTTTTTTAAAAACTTATACTTATCACGTAATCTTGTAACTAAGTTTGATTTTAATACCTACCTGTTTGTTACAAGATGCACATGAGTTTGCTAAGATCATACCAAATCACAAGTTACATATCATTGAAGGAGCTGATCATGCATACAATAATCATCAAGATGAGTTATCCTCAGTTCTTACGAGCTTCATCAAGGAAACCATTGACCACAACAAGAGTACTGCTAGCTAG
Protein sequence

>MS.gene025115.t1

MQLENKSEVKMSLSSQNPSIEHYQRIIIPNKNGEKLVGILHETGTTTDIVILCHGFRCSKDINLILNLAAALEKEQISSFRFDFSGNGESEGSFEYGNYWKEVDDLHAVAQHFRESNRVIRAIVGHSKGGDVVLLYASKYHDIKTVVNLSGRYDLKAGIEERLGKDYLKRIRKDGFFDVKRSSDAHEFAKIIPNHKLHIIEGADHAYNNHQDELSSVLTSFIKETIDHNKSTAS