AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85626

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85626.t1 MTR_2g039510 95.238 273 12 1 1 272 1 273 0.0 534
MS.gene85626.t1 MTR_2g039480 83.459 266 43 1 8 272 4 269 1.35e-165 459
MS.gene85626.t1 MTR_2g039570 80.608 263 48 2 11 272 5 265 1.27e-158 442
MS.gene85626.t1 MTR_2g039450 74.906 267 65 1 1 267 1 265 9.12e-145 408
MS.gene85626.t1 MTR_4g126050 72.075 265 69 3 1 265 57 316 4.15e-139 394
MS.gene85626.t1 MTR_4g126120 67.547 265 82 2 1 265 1 261 4.43e-135 382
MS.gene85626.t1 MTR_4g126120 67.717 254 79 1 12 265 20 270 4.77e-131 372
MS.gene85626.t1 MTR_1g016050 61.847 249 92 1 14 262 8 253 8.60e-112 323
MS.gene85626.t1 MTR_4g126050 69.730 185 51 3 1 185 57 236 6.16e-88 261
MS.gene85626.t1 MTR_1g016060 45.652 184 79 2 90 272 466 629 1.79e-38 142
MS.gene85626.t1 MTR_2g039550 51.471 68 7 1 13 80 29 70 1.19e-12 62.4
MS.gene85626.t1 MTR_4g124530 52.830 53 22 1 121 170 14 66 5.27e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85626.t1 AT3G47590 66.667 252 79 2 14 265 59 305 3.15e-123 353
MS.gene85626.t1 AT1G29840 62.977 262 92 2 4 265 52 308 2.04e-120 347
MS.gene85626.t1 AT3G47560 63.424 257 89 2 13 269 9 260 2.19e-118 340
MS.gene85626.t1 AT3G47560 63.424 257 89 2 13 269 45 296 6.40e-118 340
MS.gene85626.t1 AT3G47560 58.007 281 91 2 13 269 9 286 1.01e-114 332
MS.gene85626.t1 AT5G11910 61.355 251 94 1 12 262 20 267 2.76e-113 328
MS.gene85626.t1 AT5G11910 61.355 251 94 1 12 262 38 285 3.00e-113 328
MS.gene85626.t1 AT3G47560 57.394 284 89 3 13 269 9 287 8.12e-112 324
MS.gene85626.t1 AT3G47560 52.922 308 91 3 13 269 9 313 2.83e-108 316
MS.gene85626.t1 AT2G19550 58.203 256 95 6 15 265 5 253 7.50e-101 298
MS.gene85626.t1 AT3G47590 67.089 158 49 1 14 171 59 213 2.82e-73 224
MS.gene85626.t1 AT5G11910 61.111 144 53 1 12 155 38 178 1.40e-59 187
MS.gene85626.t1 AT5G11910 61.111 144 53 1 12 155 20 160 2.28e-59 186

Find 44 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATACTGTTGCAGGAAATT+TGG 0.190110 2.1:-48327571 MS.gene85626:intron
ATGATATTTAACTTGTGATT+TGG 0.296012 2.1:+48326934 None:intergenic
CGTTTGAGTACGGTAACTAT+TGG 0.338058 2.1:-48328604 MS.gene85626:CDS
AACTCATGTGCATCTTGAAC+CGG 0.377677 2.1:+48326970 None:intergenic
TTTGATGTGAAGAGGAGTTC+AGG 0.389844 2.1:-48327822 MS.gene85626:intron
AAGATTATTTGAAAAGAATT+CGG 0.389987 2.1:-48327857 MS.gene85626:CDS
GGAATATTACATGAATGTTC+TGG 0.393067 2.1:-48330001 MS.gene85626:CDS
CAGAAGAACCATGAATTGTT+AGG 0.417355 2.1:+48327004 None:intergenic
AAAACTGTTGTTAACCTCTC+TGG 0.430009 2.1:-48327921 MS.gene85626:CDS
GACAGGGTCCTAACAATTCA+TGG 0.435771 2.1:-48327012 MS.gene85626:intron
AGCAGTACTCTTGTGGTCAA+TGG 0.439676 2.1:+48326837 None:intergenic
TGTTACTGAGGAAAGTTTGA+TGG 0.439937 2.1:-48327541 MS.gene85626:CDS
CGTGTGATCCGTGCAATTGT+TGG 0.442805 2.1:-48328530 MS.gene85626:CDS
TGCAGATTGACAAAGATTGC+AGG 0.447725 2.1:-48327485 MS.gene85626:intron
ACTGAACCTTGCTGCTGCAT+TGG 0.448457 2.1:-48329665 MS.gene85626:CDS
CCACAAGAGTACTGCTAGCT+AGG 0.453279 2.1:-48326830 None:intergenic
CCTTGCTGCTGCATTGGAAA+AGG 0.467817 2.1:-48329659 MS.gene85626:CDS
CACAAGTTAAATATCATTGA+AGG 0.469307 2.1:-48326928 MS.gene85626:CDS
TGCAATCTTTGTCAATCTGC+AGG 0.492750 2.1:+48327487 None:intergenic
GGATCACACGATTTGATTCA+CGG 0.506479 2.1:+48328543 None:intergenic
GACATTGTTATCTTATGTCA+TGG 0.507199 2.1:-48329965 MS.gene85626:CDS
AGTTCTTGTGAGCTTCATCA+AGG 0.518328 2.1:-48326863 MS.gene85626:CDS
ATCATAATACCCAACAAAAG+TGG 0.530706 2.1:-48330037 MS.gene85626:CDS
TTCTTCTGACGAAATTATCC+CGG 0.538226 2.1:-48326989 MS.gene85626:CDS
ACGGTTTCTTTGATGTGAAG+AGG 0.543472 2.1:-48327830 MS.gene85626:CDS
GCAATTGTTGGACATAGTAA+AGG 0.544207 2.1:-48328518 MS.gene85626:intron
TACTATGTCCAACAATTGCA+CGG 0.547440 2.1:+48328522 None:intergenic
TTGAAAAGAATTCGGAAGGA+CGG 0.548995 2.1:-48327849 MS.gene85626:CDS
CTCTGGACGTTATGATCTGA+AGG 0.550517 2.1:-48327904 MS.gene85626:CDS
AGCGAAGGTTCGTTTGAGTA+CGG 0.557534 2.1:-48328614 MS.gene85626:CDS
GGACGTTATGATCTGAAGGC+AGG 0.563099 2.1:-48327900 MS.gene85626:CDS
CGGTAACTATTGGAAAGAAG+TGG 0.564899 2.1:-48328594 MS.gene85626:CDS
ACTCATGTGCATCTTGAACC+GGG 0.566619 2.1:+48326971 None:intergenic
TTATTTGAAAAGAATTCGGA+AGG 0.579693 2.1:-48327853 MS.gene85626:CDS
ACATAAGATAACAATGTCGT+TGG 0.580798 2.1:+48329970 None:intergenic
CATTTCCAGAAAAGTCAAAA+CGG 0.584654 2.1:+48329620 None:intergenic
GCAGGCATTGAAGAACGCCT+TGG 0.597706 2.1:-48327882 MS.gene85626:CDS
CCTAGCTAGCAGTACTCTTG+TGG 0.599369 2.1:+48326830 None:intergenic
GATATTTGGAAGCATACAGA+AGG 0.625385 2.1:+48327952 None:intergenic
TTTGGACTACCGTGTTACTG+AGG 0.647039 2.1:-48327553 MS.gene85626:CDS
TTTCCTTCAAGGGAAAGCGA+AGG 0.664955 2.1:-48328629 MS.gene85626:intron
TCAAACTTTCCTCAGTAACA+CGG 0.679801 2.1:+48327544 None:intergenic
GATGAAGCTCACAAGAACTG+AGG 0.686040 2.1:+48326867 None:intergenic
TCAGATCATAACGTCCAGAG+AGG 0.713623 2.1:+48327907 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAGTTTTTAAAAAAAGTA+TGG + chr2.1:48329720-48329739 None:intergenic 10.0%
!! AAATGAATATTCAGTTAATA+TGG + chr2.1:48327549-48327568 None:intergenic 15.0%
!! AAGATTATTTGAAAAGAATT+CGG - chr2.1:48329139-48329158 MS.gene85626:intron 15.0%
!! ATTCATTAAATGATCATTTA+AGG - chr2.1:48329400-48329419 MS.gene85626:intron 15.0%
!! GTAATAATTTGAGAAAAAAA+TGG + chr2.1:48327824-48327843 None:intergenic 15.0%
!! AAAAAAAAGAAGAAGAAGAA+AGG + chr2.1:48327080-48327099 None:intergenic 20.0%
!! AAAATATAAGTCAATCCAAA+CGG - chr2.1:48327506-48327525 MS.gene85626:CDS 20.0%
!! AATACCATGATAAAATGATA+AGG + chr2.1:48328210-48328229 None:intergenic 20.0%
!! ACATAAATTTATTGTACATG+AGG + chr2.1:48328270-48328289 None:intergenic 20.0%
!! ATCTGTATAATGATATTTGT+TGG - chr2.1:48328905-48328924 MS.gene85626:intron 20.0%
!! CAATGAATGATAGTATAAAT+TGG + chr2.1:48328588-48328607 None:intergenic 20.0%
!! GATAAAATGATAAGGAAATT+GGG + chr2.1:48328202-48328221 None:intergenic 20.0%
!! TAAACCAATTAATCATCTTT+CGG + chr2.1:48327930-48327949 None:intergenic 20.0%
!! TACATAAGAATTTGAGAAAA+TGG + chr2.1:48328948-48328967 None:intergenic 20.0%
!! TGATAAAATGATAAGGAAAT+TGG + chr2.1:48328203-48328222 None:intergenic 20.0%
!!! ATACCTATTTGTTAAAATGA+AGG + chr2.1:48328878-48328897 None:intergenic 20.0%
!!! ATGATATTTAACTTGTGATT+TGG + chr2.1:48330065-48330084 None:intergenic 20.0%
!!! ATTTCCTTATCATTTTATCA+TGG - chr2.1:48328203-48328222 MS.gene85626:intron 20.0%
!!! GGTTTTTAGTTTAGAATTAT+TGG - chr2.1:48328142-48328161 MS.gene85626:intron 20.0%
!!! TATCCTTCATTTTAACAAAT+AGG - chr2.1:48328872-48328891 MS.gene85626:intron 20.0%
!!! TCAATTTTCATTATCCATAA+AGG + chr2.1:48329325-48329344 None:intergenic 20.0%
!!! TTAGAACTTTAGAATTCAAA+TGG - chr2.1:48329295-48329314 MS.gene85626:intron 20.0%
! AAATATAAGTCAATCCAAAC+GGG - chr2.1:48327507-48327526 MS.gene85626:CDS 25.0%
! AACATGAAGATGTTAAGTTA+TGG - chr2.1:48328539-48328558 MS.gene85626:CDS 25.0%
! ATAGTGCTACATATGATATA+TGG - chr2.1:48329863-48329882 MS.gene85626:intron 25.0%
! CAAAAATAAGTCAATCCAAA+CGG + chr2.1:48327426-48327445 None:intergenic 25.0%
! CACAAGTTAAATATCATTGA+AGG - chr2.1:48330068-48330087 MS.gene85626:intron 25.0%
! CATTCATTAAATGATGTATG+TGG - chr2.1:48327207-48327226 MS.gene85626:intron 25.0%
! GATTTATACTGCAAAATGAT+AGG - chr2.1:48327631-48327650 MS.gene85626:intron 25.0%
! TATATCCAAACTCAAAACAT+AGG + chr2.1:48327967-48327986 None:intergenic 25.0%
! TTATTTGAAAAGAATTCGGA+AGG - chr2.1:48329143-48329162 MS.gene85626:intron 25.0%
! TTGAATGTAAGAAACTAACA+TGG + chr2.1:48326855-48326874 None:intergenic 25.0%
!! AAGATGTTAAGTTATGGTTT+TGG - chr2.1:48328545-48328564 MS.gene85626:CDS 25.0%
!! ACAATTCCTTTTCCAAAATT+GGG - chr2.1:48328235-48328254 MS.gene85626:intron 25.0%
!! ACATGAGCATTGTATTAATT+TGG - chr2.1:48329376-48329395 MS.gene85626:intron 25.0%
!! ATTCAAATGGATTTCCTTTA+TGG - chr2.1:48329308-48329327 MS.gene85626:intron 25.0%
!! TGATTACTTTGAAAACAAAC+AGG + chr2.1:48329786-48329805 None:intergenic 25.0%
!! TTTTCAAATAATCTTTGCCA+AGG + chr2.1:48329134-48329153 None:intergenic 25.0%
!!! AGTTTTAATGTCGTGATATT+TGG + chr2.1:48329061-48329080 None:intergenic 25.0%
!!! GTATATTTTTAGGATTGATG+TGG - chr2.1:48327698-48327717 MS.gene85626:intron 25.0%
!!! TATGTTTTGAGTTTGGATAT+AGG - chr2.1:48327966-48327985 MS.gene85626:CDS 25.0%
!!! TTGAACTTATGCTTTTTAGT+GGG - chr2.1:48327882-48327901 MS.gene85626:CDS 25.0%
!!! TTTGAACTTATGCTTTTTAG+TGG - chr2.1:48327881-48327900 MS.gene85626:CDS 25.0%
AAAAGTGGTGAAAAACTTGT+TGG - chr2.1:48326974-48326993 MS.gene85626:CDS 30.0%
AAGCTACATCATATATGACA+GGG - chr2.1:48329968-48329987 MS.gene85626:CDS 30.0%
ACATAAGATAACAATGTCGT+TGG + chr2.1:48327029-48327048 None:intergenic 30.0%
ATAGCCGAAAGATGATTAAT+TGG - chr2.1:48327923-48327942 MS.gene85626:CDS 30.0%
ATCATAATACCCAACAAAAG+TGG - chr2.1:48326959-48326978 MS.gene85626:CDS 30.0%
CAATCTTAACATGCAAAGTA+TGG + chr2.1:48328671-48328690 None:intergenic 30.0%
CATTTCCAGAAAAGTCAAAA+CGG + chr2.1:48327379-48327398 None:intergenic 30.0%
GACATTGTTATCTTATGTCA+TGG - chr2.1:48327031-48327050 MS.gene85626:intron 30.0%
GATGAATGAACTGAATCATA+TGG - chr2.1:48328001-48328020 MS.gene85626:intron 30.0%
GGAATATTACATGAATGTTC+TGG - chr2.1:48326995-48327014 MS.gene85626:CDS 30.0%
GTCATAATTGAGGTGTATAA+TGG + chr2.1:48328619-48328638 None:intergenic 30.0%
! AGGAAAATACTACCCAATTT+TGG + chr2.1:48328250-48328269 None:intergenic 30.0%
! GACAATTCCTTTTCCAAAAT+TGG - chr2.1:48328234-48328253 MS.gene85626:intron 30.0%
! GTTTTGCTTATAATAGTGAC+CGG - chr2.1:48327281-48327300 MS.gene85626:intron 30.0%
! GTTTTGCTTATAATAGTGAC+CGG + chr2.1:48327300-48327281 None:intergenic 30.0%
! TACTTTGAAAACAAACAGGT+AGG + chr2.1:48329782-48329801 None:intergenic 30.0%
! TGGACATAGTAAAGGTATAT+TGG - chr2.1:48328486-48328505 MS.gene85626:intron 30.0%
!! AAGTTTTTCACCACTTTTGT+TGG + chr2.1:48326972-48326991 None:intergenic 30.0%
!! AGTTTTTCACCACTTTTGTT+GGG + chr2.1:48326971-48326990 None:intergenic 30.0%
!! ATACTACCCAATTTTGGAAA+AGG + chr2.1:48328244-48328263 None:intergenic 30.0%
!! TGAACTTATGCTTTTTAGTG+GGG - chr2.1:48327883-48327902 MS.gene85626:CDS 30.0%
!! TTCTCTTAACTTTAGCTTGT+GGG - chr2.1:48328343-48328362 MS.gene85626:intron 30.0%
!! TTGGAAGGGTGTATATTTTT+AGG - chr2.1:48327688-48327707 MS.gene85626:intron 30.0%
!! TTTCTCTTAACTTTAGCTTG+TGG - chr2.1:48328342-48328361 MS.gene85626:intron 30.0%
!!! ATTTGCCTATGTTTTGAGTT+TGG - chr2.1:48327959-48327978 MS.gene85626:CDS 30.0%
!!! GATGAAGTTCATTTTTAGCA+TGG + chr2.1:48328850-48328869 None:intergenic 30.0%
!!! GTTTTGACTTTTCTGGAAAT+GGG - chr2.1:48327378-48327397 MS.gene85626:intron 30.0%
AAAACTGTTGTTAACCTCTC+TGG - chr2.1:48329075-48329094 MS.gene85626:intron 35.0%
AAAAGGAGAAGAGATCAAAC+AGG + chr2.1:48329548-48329567 None:intergenic 35.0%
ACATACTGTTGCAGGAAATT+TGG - chr2.1:48329425-48329444 MS.gene85626:intron 35.0%
AGAAAGCAAAGAATGAAGCA+AGG + chr2.1:48328327-48328346 None:intergenic 35.0%
ATGATGATTTAGTGTCCGTT+TGG - chr2.1:48327408-48327427 MS.gene85626:intron 35.0%
CAAGCTACATCATATATGAC+AGG - chr2.1:48329967-48329986 MS.gene85626:CDS 35.0%
CAGAAGAACCATGAATTGTT+AGG + chr2.1:48329995-48330014 None:intergenic 35.0%
CATGCTTGAACAACAATGAA+TGG + chr2.1:48329581-48329600 None:intergenic 35.0%
CTACATATGATATATGGCGA+AGG - chr2.1:48329869-48329888 MS.gene85626:intron 35.0%
CTGTCATATATGATGTAGCT+TGG + chr2.1:48329969-48329988 None:intergenic 35.0%
GACACTTCTTGTCATAATTG+AGG + chr2.1:48328629-48328648 None:intergenic 35.0%
GATAAATGAAAGCTCATTCC+TGG + chr2.1:48328751-48328770 None:intergenic 35.0%
GCAATTGTTGGACATAGTAA+AGG - chr2.1:48328478-48328497 MS.gene85626:intron 35.0%
GTAGACTATAAAGAACACAC+TGG + chr2.1:48327858-48327877 None:intergenic 35.0%
TACTATGTCCAACAATTGCA+CGG + chr2.1:48328477-48328496 None:intergenic 35.0%
TCAAACTTTCCTCAGTAACA+CGG + chr2.1:48329455-48329474 None:intergenic 35.0%
TCTTTCTATTATACACCCTC+CGG - chr2.1:48327150-48327169 MS.gene85626:intron 35.0%
TGACAAAGATTGCAGGTTAA+TGG - chr2.1:48329518-48329537 MS.gene85626:intron 35.0%
TGTTACTGAGGAAAGTTTGA+TGG - chr2.1:48329455-48329474 MS.gene85626:intron 35.0%
TGTTATTACGCTTACAGTTC+TGG - chr2.1:48328121-48328140 MS.gene85626:intron 35.0%
TTCAGGTAAATCGTTTACTC+AGG - chr2.1:48329191-48329210 MS.gene85626:intron 35.0%
TTCTTCTGACGAAATTATCC+CGG - chr2.1:48330007-48330026 MS.gene85626:CDS 35.0%
TTGAAAAGAATTCGGAAGGA+CGG - chr2.1:48329147-48329166 MS.gene85626:intron 35.0%
! GATATTTGGAAGCATACAGA+AGG + chr2.1:48329047-48329066 None:intergenic 35.0%
!! ATGCTTTTTAGTGGGGTATT+GGG - chr2.1:48327890-48327909 MS.gene85626:CDS 35.0%
!! TATGCTTTTTAGTGGGGTAT+TGG - chr2.1:48327889-48327908 MS.gene85626:CDS 35.0%
!!! CGTTTTGACTTTTCTGGAAA+TGG - chr2.1:48327377-48327396 MS.gene85626:intron 35.0%
!!! TCTTTCCGTTTTGACTTTTC+TGG - chr2.1:48327371-48327390 MS.gene85626:intron 35.0%
AACTCATGTGCATCTTGAAC+CGG + chr2.1:48330029-48330048 None:intergenic 40.0%
ACCAAAGCAAACACATCAGA+CGG + chr2.1:48328058-48328077 None:intergenic 40.0%
ACGGTTTCTTTGATGTGAAG+AGG - chr2.1:48329166-48329185 MS.gene85626:intron 40.0%
AGGATTGATGTGGTTTCATG+TGG - chr2.1:48327708-48327727 MS.gene85626:intron 40.0%
AGTTCTTGTGAGCTTCATCA+AGG - chr2.1:48330133-48330152 MS.gene85626:intron 40.0%
ATAAGGAAATTGGGTCACTG+AGG + chr2.1:48328193-48328212 None:intergenic 40.0%
CCATCATCCTAGCAAACTTT+GGG + chr2.1:48328977-48328996 None:intergenic 40.0%
CGGTAACTATTGGAAAGAAG+TGG - chr2.1:48328402-48328421 MS.gene85626:intron 40.0%
CGTTTGAGTACGGTAACTAT+TGG - chr2.1:48328392-48328411 MS.gene85626:intron 40.0%
GAAGAGATTTGCGTAACTTC+TGG - chr2.1:48328712-48328731 MS.gene85626:intron 40.0%
GATATCCTATATACACCCTC+CGG + chr2.1:48327303-48327322 None:intergenic 40.0%
GGATCACACGATTTGATTCA+CGG + chr2.1:48328456-48328475 None:intergenic 40.0%
TCCATCATCCTAGCAAACTT+TGG + chr2.1:48328978-48328997 None:intergenic 40.0%
TCTGGCAAAAAACATACACC+AGG - chr2.1:48328730-48328749 MS.gene85626:intron 40.0%
TGAATGGAGCTAGCAGTAAA+AGG + chr2.1:48329565-48329584 None:intergenic 40.0%
TGCAATCTTTGTCAATCTGC+AGG + chr2.1:48329512-48329531 None:intergenic 40.0%
TGCAGATTGACAAAGATTGC+AGG - chr2.1:48329511-48329530 MS.gene85626:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr2.1:48327285-48327304 MS.gene85626:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr2.1:48327304-48327285 None:intergenic 40.0%
TTAAGGACACATACTGTTGC+AGG - chr2.1:48329417-48329436 MS.gene85626:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr2.1:48327284-48327303 MS.gene85626:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr2.1:48327303-48327284 None:intergenic 40.0%
TTTGATGTGAAGAGGAGTTC+AGG - chr2.1:48329174-48329193 MS.gene85626:intron 40.0%
! ACTTTCACCCAAAGTTTGCT+AGG - chr2.1:48328967-48328986 MS.gene85626:intron 40.0%
! TTTGAGTTTGGATATAGGCG+TGG - chr2.1:48327971-48327990 MS.gene85626:intron 40.0%
!! TCCTGGTTGTTCTTTTGGAA+GGG - chr2.1:48327674-48327693 MS.gene85626:intron 40.0%
!! TTGTTTCTGTGTCATGATGC+AGG - chr2.1:48329009-48329028 MS.gene85626:intron 40.0%
!!! TGCTTTTTAGTGGGGTATTG+GGG - chr2.1:48327891-48327910 MS.gene85626:CDS 40.0%
AAGAACAACCAGGACAGAGA+TGG + chr2.1:48327668-48327687 None:intergenic 45.0%
ACCCTTCCAAAAGAACAACC+AGG + chr2.1:48327678-48327697 None:intergenic 45.0%
ACTCATGTGCATCTTGAACC+GGG + chr2.1:48330028-48330047 None:intergenic 45.0%
AGCAGTACTCTTGTGGTCAA+TGG + chr2.1:48330162-48330181 None:intergenic 45.0%
AGCGAAGGTTCGTTTGAGTA+CGG - chr2.1:48328382-48328401 MS.gene85626:intron 45.0%
CACACTGACATGCCTTCAAT+TGG + chr2.1:48327766-48327785 None:intergenic 45.0%
CATGCCTTCAATTGGAATGC+AGG + chr2.1:48327758-48327777 None:intergenic 45.0%
CCAAAGCAAACACATCAGAC+GGG + chr2.1:48328057-48328076 None:intergenic 45.0%
CTCTGGACGTTATGATCTGA+AGG - chr2.1:48329092-48329111 MS.gene85626:intron 45.0%
GACACCTGCATTCCAATTGA+AGG - chr2.1:48327751-48327770 MS.gene85626:intron 45.0%
GACAGGGTCCTAACAATTCA+TGG - chr2.1:48329984-48330003 MS.gene85626:CDS 45.0%
GATGAAGCTCACAAGAACTG+AGG + chr2.1:48330132-48330151 None:intergenic 45.0%
TCAGATCATAACGTCCAGAG+AGG + chr2.1:48329092-48329111 None:intergenic 45.0%
TGCAGGTGTCAGTGCTATAT+AGG + chr2.1:48327741-48327760 None:intergenic 45.0%
TTTCCTTCAAGGGAAAGCGA+AGG - chr2.1:48328367-48328386 MS.gene85626:intron 45.0%
TTTCTGTGTCATGATGCAGG+AGG - chr2.1:48329012-48329031 MS.gene85626:intron 45.0%
! AGCTTGTGGGTTTTCCTTCA+AGG - chr2.1:48328356-48328375 MS.gene85626:intron 45.0%
! CTCTGTCCTGGTTGTTCTTT+TGG - chr2.1:48327669-48327688 MS.gene85626:intron 45.0%
! GCTTGTGGGTTTTCCTTCAA+GGG - chr2.1:48328357-48328376 MS.gene85626:intron 45.0%
! GTCCTGGTTGTTCTTTTGGA+AGG - chr2.1:48327673-48327692 MS.gene85626:intron 45.0%
! TTTGGACTACCGTGTTACTG+AGG - chr2.1:48329443-48329462 MS.gene85626:intron 45.0%
!! CCCAAAGTTTGCTAGGATGA+TGG - chr2.1:48328974-48328993 MS.gene85626:intron 45.0%
!!! GCTTTTTAGTGGGGTATTGG+GGG - chr2.1:48327892-48327911 MS.gene85626:CDS 45.0%
AAGCAAACACATCAGACGGG+CGG + chr2.1:48328054-48328073 None:intergenic 50.0%
ACTGAACCTTGCTGCTGCAT+TGG - chr2.1:48327331-48327350 MS.gene85626:intron 50.0%
CCCGTCTGATGTGTTTGCTT+TGG - chr2.1:48328054-48328073 MS.gene85626:intron 50.0%
CCTTGCTGCTGCATTGGAAA+AGG - chr2.1:48327337-48327356 MS.gene85626:intron 50.0%
CGTGTGATCCGTGCAATTGT+TGG - chr2.1:48328466-48328485 MS.gene85626:intron 50.0%
GAACCTTCGCTTTCCCTTGA+AGG + chr2.1:48328373-48328392 None:intergenic 50.0%
GGACGTTATGATCTGAAGGC+AGG - chr2.1:48329096-48329115 MS.gene85626:intron 50.0%
GTGACATCGACACACACATC+TGG - chr2.1:48327783-48327802 MS.gene85626:intron 50.0%
TGGATATAGGCGTGGATCAC+TGG - chr2.1:48327979-48327998 MS.gene85626:intron 50.0%
! AGTGACCGGAGGGTGTATAT+AGG - chr2.1:48327295-48327314 MS.gene85626:intron 50.0%
! CCTTTTCCAATGCAGCAGCA+AGG + chr2.1:48327340-48327359 None:intergenic 50.0%
AGACCAAGCCATCTCTGTCC+TGG - chr2.1:48327657-48327676 MS.gene85626:intron 55.0%
CAACCAGGACAGAGATGGCT+TGG + chr2.1:48327663-48327682 None:intergenic 55.0%
GAACACACTGGACACGACAC+TGG + chr2.1:48327846-48327865 None:intergenic 55.0%
GCGTTCACTAAGAGCCCGTT+TGG + chr2.1:48327524-48327543 None:intergenic 55.0%
!! GCAGGCATTGAAGAACGCCT+TGG - chr2.1:48329114-48329133 MS.gene85626:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 48326831 48330187 48326831 ID=MS.gene85626
chr2.1 mRNA 48326831 48330187 48326831 ID=MS.gene85626.t1;Parent=MS.gene85626
chr2.1 exon 48330163 48330187 48330163 ID=MS.gene85626.t1.exon1;Parent=MS.gene85626.t1
chr2.1 CDS 48330163 48330187 48330163 ID=cds.MS.gene85626.t1;Parent=MS.gene85626.t1
chr2.1 exon 48329949 48330079 48329949 ID=MS.gene85626.t1.exon2;Parent=MS.gene85626.t1
chr2.1 CDS 48329949 48330079 48329949 ID=cds.MS.gene85626.t1;Parent=MS.gene85626.t1
chr2.1 exon 48329619 48329701 48329619 ID=MS.gene85626.t1.exon3;Parent=MS.gene85626.t1
chr2.1 CDS 48329619 48329701 48329619 ID=cds.MS.gene85626.t1;Parent=MS.gene85626.t1
chr2.1 exon 48328519 48328640 48328519 ID=MS.gene85626.t1.exon4;Parent=MS.gene85626.t1
chr2.1 CDS 48328519 48328640 48328519 ID=cds.MS.gene85626.t1;Parent=MS.gene85626.t1
chr2.1 exon 48327823 48327987 48327823 ID=MS.gene85626.t1.exon5;Parent=MS.gene85626.t1
chr2.1 CDS 48327823 48327987 48327823 ID=cds.MS.gene85626.t1;Parent=MS.gene85626.t1
chr2.1 exon 48327486 48327579 48327486 ID=MS.gene85626.t1.exon6;Parent=MS.gene85626.t1
chr2.1 CDS 48327486 48327579 48327486 ID=cds.MS.gene85626.t1;Parent=MS.gene85626.t1
chr2.1 exon 48326831 48327029 48326831 ID=MS.gene85626.t1.exon7;Parent=MS.gene85626.t1
chr2.1 CDS 48326831 48327029 48326831 ID=cds.MS.gene85626.t1;Parent=MS.gene85626.t1
Gene Sequence

>MS.gene85626

ATGTCACTAGCTTCACAAAATCCATGTTAGTTTCTTACATTCAATATAAACAAAAAATTTATAAAAAAAATTGATGAATGATTTATTATTTCTTATTTGTTTTTGTAGCTATTGAGCATCATCAAAGAATCATAATACCCAACAAAAGTGGTGAAAAACTTGTTGGAATATTACATGAATGTTCTGGAACAACAACCAACGACATTGTTATCTTATGTCATGGTTTTCGATGCTCTAAAGTTTGTTCCTTTCTTCTTCTTCTTTTTTTTCTTTTTTACTTAATTTATGAATAAATTCTTTTACTAATTTGTATTTTTTTTCTTTCTATTATACACCCTCCGGTCACTATTATAAGCAAAACTTTGTATTTTACATTCATTCATTAAATGATGTATGTGGTCTATAATAAAGACTACATACATCATTAATTGAATGTATATAAAATGTAAAGTTTTGCTTATAATAGTGACCGGAGGGTGTATATAGGATATCAATCTCATACTGAACCTTGCTGCTGCATTGGAAAAGGAACAAATTAGTTCTTTCCGTTTTGACTTTTCTGGAAATGGGTATGTTTATGATGATTTAGTGTCCGTTTGGATTGACTTATTTTTGAGCTTATGCAAAACAGCTTATGAAGATACAATTTTCGTTAGTTATTTATTTACATAAGCTAAAATATAAGTCAATCCAAACGGGCTCTTAGTGAACGCAACCATATTAACTGAATATTCATTTTGACTACATTTTGATATATAGTCAATCGCATTCTAAATGTTTGTGAAATTGTCTCTAAAATTGATTTATACTGCAAAATGATAGGCTAAGACCAAGCCATCTCTGTCCTGGTTGTTCTTTTGGAAGGGTGTATATTTTTAGGATTGATGTGGTTTCATGTGGTTTATGACCTATATAGCACTGACACCTGCATTCCAATTGAAGGCATGTCAGTGTGACATCGACACACACATCTGGTTACATTCAGTTATTCCATTTTTTTCTCAAATTATTACCAGTGTCGTGTCCAGTGTGTTCTTTATAGTCTACAATTTTGAACTTATGCTTTTTAGTGGGGTATTGGGGGAGTTGATAATAGCCGAAAGATGATTAATTGGTTTACTTAAAAGCATTTGCCTATGTTTTGAGTTTGGATATAGGCGTGGATCACTGGATGAATGAACTGAATCATATGGTAGTATGATCACTAAAATAGTGTGTTTCCGCCCGTCTGATGTGTTTGCTTTGGTTTGCTGATGTTATTTATACTTATTTATCATAATTATCTAATGTTGTTATTACGCTTACAGTTCTGGTTTTTAGTTTAGAATTATTGGTTTAGTTGTAGTACTATTGTACTATCCTCAGTGACCCAATTTCCTTATCATTTTATCATGGTATTAGATGACAATTCCTTTTCCAAAATTGGGTAGTATTTTCCTCATGTACAATAAATTTATGTACTAAAGTCTATGCTATCTTGTTTTCATCATTTGCCTTGCTTCATTCTTTGCTTTCTCTTAACTTTAGCTTGTGGGTTTTCCTTCAAGGGAAAGCGAAGGTTCGTTTGAGTACGGTAACTATTGGAAAGAAGTGGATGATTTACATGCTGTTGCTCAACATTTCCGTGAATCAAATCGTGTGATCCGTGCAATTGTTGGACATAGTAAAGGTATATTGGTTTGTTTGCTTATATTGAAGTATTTTAAGCAACATGAAGATGTTAAGTTATGGTTTTGGATATATGATTATGTGCTCCAATTTATACTATCATTCATTGCTAATTTTCCATTATACACCTCAATTATGACAAGAAGTGTCGTTATAAAAATATTGTCAACCATACTTTGCATGTTAAGATTGCAATGTTGAATGATGCTTTATGAAGAGATTTGCGTAACTTCTGGCAAAAAACATACACCAGGAATGAGCTTTCATTTATCTGATATATAGCTTCTAGCATGAGATTTGTCAATAAGTCGAAAGTTACTATTCTACAAATGAACTTCATTTGCAAAACCATGCTAAAAATGAACTTCATCATTATCCTTCATTTTAACAAATAGGTATTCTATAGATCTGTATAATGATATTTGTTGGTGTAGCTTCTGATCAATCCATTTTCTCAAATTCTTATGTACTTTCACCCAAAGTTTGCTAGGATGATGGAACTGTTAACAGTTGTTTCTGTGTCATGATGCAGGAGGTGATGTAGTCCTTCTGTATGCTTCCAAATATCACGACATTAAAACTGTTGTTAACCTCTCTGGACGTTATGATCTGAAGGCAGGCATTGAAGAACGCCTTGGCAAAGATTATTTGAAAAGAATTCGGAAGGACGGTTTCTTTGATGTGAAGAGGAGTTCAGGTAAATCGTTTACTCAGGCTAAAAATATCACAAGTACAAAGTTCGAGTTAGACATTCATCGTGAACTGTCTTGTTCAATAACTGTAAAATGACTTGAGTTTAGAACTTTAGAATTCAAATGGATTTCCTTTATGGATAATGAAAATTGACAAGTTCTTTGTTTCAGTCTCTATCACTTACACATGAGCATTGTATTAATTTGGTATTCATTAAATGATCATTTAAGGACACATACTGTTGCAGGAAATTTGGACTACCGTGTTACTGAGGAAAGTTTGATGGATCGCTTGAGTACAAATATGCATGAAGCGTGCCTGCAGATTGACAAAGATTGCAGGTTAATGGAAAACCTGTTTGATCTCTTCTCCTTTTACTGCTAGCTCCATTCATTGTTGTTCAAGCATGCGTCGACGTTGAAATATCACAATAATATTTCTACAATAGAAGCAATAAAAAACATGATATCTCTCAATTCTGATTATTCTTTCATTACAATTACGTGCAATTTAACTACAACATTTCCATACTTTTTTTAAAAACTTATACTTATCACGTAATCTTGTAACTAAGTTTGATTTTAATACCTACCTGTTTGTTTTCAAAGTAATCATTGTAAATAATACAAAATAATGCTTTTAAGATATTCAAGTTTCTTTAAATAATTGAAATAGTGCTACATATGATATATGGCGAAGGAGCAGAGAAGACTGCAAGCAACAATTCTTGAACTTATCAAGTTACATTGATGCATATGATATATACTGTTAATGCCAAGCTACATCATATATGACAGGGTCCTAACAATTCATGGTTCTTCTGACGAAATTATCCCGGTTCAAGATGCACATGAGTTTGCTAAGATCATACCAAATCACAAGTTAAATATCATTGAAGGAGCTGATCATGCATACAATAATCATCAAGATGAGTTATCCTCAGTTCTTGTGAGCTTCATCAAGGAAACCATTGACCACAAGAGTACTGCTAGCTAG
Protein sequence

>MS.gene85626.t1

MSLASQNPSIEHHQRIIIPNKSGEKLVGILHECSGTTTNDIVILCHGFRCSKDINLILNLAAALEKEQISSFRFDFSGNGESEGSFEYGNYWKEVDDLHAVAQHFRESNRVIRAIVGHSKGGDVVLLYASKYHDIKTVVNLSGRYDLKAGIEERLGKDYLKRIRKDGFFDVKRSSGNLDYRVTEESLMDRLSTNMHEACLQIDKDCRVLTIHGSSDEIIPVQDAHEFAKIIPNHKLNIIEGADHAYNNHQDELSSVLVSFIKETIDHKSTAS