AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026086

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026086.t1 MTR_2g039570 91.698 265 22 0 6 270 1 265 0.0 504
MS.gene026086.t1 MTR_2g039510 82.510 263 44 1 10 270 11 273 8.50e-164 455
MS.gene026086.t1 MTR_2g039480 76.046 263 61 1 10 270 7 269 1.18e-146 411
MS.gene026086.t1 MTR_2g039450 71.206 257 74 0 10 266 11 267 2.58e-133 379
MS.gene026086.t1 MTR_4g126050 69.349 261 78 2 8 268 64 322 1.35e-132 378
MS.gene026086.t1 MTR_4g126120 61.450 262 100 1 1 262 1 261 2.28e-124 355
MS.gene026086.t1 MTR_4g126120 63.492 252 91 1 11 262 20 270 1.29e-123 353
MS.gene026086.t1 MTR_1g016050 58.871 248 101 1 12 259 7 253 5.48e-106 309
MS.gene026086.t1 MTR_4g126050 70.455 176 50 2 8 183 64 237 5.92e-86 256
MS.gene026086.t1 MTR_1g016060 42.308 182 84 2 88 268 467 628 4.39e-33 127
MS.gene026086.t1 MTR_2g039550 57.143 77 9 1 1 77 18 70 3.83e-19 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026086.t1 AT3G47590 63.320 259 92 2 7 265 53 308 6.41e-120 345
MS.gene026086.t1 AT1G29840 60.377 265 102 2 1 265 50 311 4.15e-117 338
MS.gene026086.t1 AT3G47560 61.390 259 94 3 1 259 1 253 1.94e-114 330
MS.gene026086.t1 AT3G47560 61.390 259 94 3 1 259 37 289 5.62e-114 330
MS.gene026086.t1 AT5G11910 58.140 258 107 1 2 259 11 267 6.30e-111 322
MS.gene026086.t1 AT5G11910 58.140 258 107 1 2 259 29 285 1.12e-110 322
MS.gene026086.t1 AT3G47560 56.184 283 96 3 1 259 1 279 1.66e-110 321
MS.gene026086.t1 AT3G47560 55.594 286 94 4 1 259 1 280 5.36e-108 314
MS.gene026086.t1 AT3G47560 51.290 310 96 4 1 259 1 306 4.38e-104 305
MS.gene026086.t1 AT2G19550 54.475 257 107 6 11 262 2 253 3.97e-96 286
MS.gene026086.t1 AT3G47590 66.049 162 54 1 7 168 53 213 8.65e-76 230
MS.gene026086.t1 AT5G11910 59.603 151 60 1 2 152 11 160 2.86e-60 188
MS.gene026086.t1 AT5G11910 59.603 151 60 1 2 152 29 178 2.86e-60 189

Find 46 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCCTTGGTAAAGATTATT+TGG 0.161587 2.2:+47928246 MS.gene026086:CDS
TGTTACTAAGGAAAGTTTAA+TGG 0.281740 2.2:+47928583 MS.gene026086:CDS
ATCTACTGTTGCAGGAAGTT+TGG 0.288735 2.2:+47928553 MS.gene026086:intron
CGTTCCAGTATGGTAACTAT+TGG 0.315195 2.2:+47926743 MS.gene026086:CDS
GTTCCAGTATGGTAACTATT+GGG 0.346161 2.2:+47926744 MS.gene026086:CDS
CGTGTAATCCGTGCAATTGT+TGG 0.348094 2.2:+47926817 MS.gene026086:CDS
GGAAACCATTGACCACAATA+AGG 0.355010 2.2:+47929146 MS.gene026086:CDS
ATGATATGCAACTTGTGATT+TGG 0.362877 2.2:-47929054 None:intergenic
TTCAGATTCACAAAGATTGC+AGG 0.382227 2.2:+47928639 MS.gene026086:CDS
GTTGGCATATTGCATGAATC+TGG 0.383653 2.2:+47925995 MS.gene026086:CDS
AACTCGTGTGCACTTTGAAC+GGG 0.393008 2.2:-47929018 None:intergenic
GTTGAACCTTGTTGCTGCAT+TGG 0.422568 2.2:+47926167 MS.gene026086:CDS
AGTACCCTTATTGTGGTCAA+TGG 0.432514 2.2:-47929151 None:intergenic
ATTGATGTGAAGACGAAGTC+AGG 0.444727 2.2:+47928292 MS.gene026086:CDS
AGCGAAGGTTCGTTCCAGTA+TGG 0.446124 2.2:+47926733 MS.gene026086:CDS
TTTGGATTACCGTGTTACTA+AGG 0.456099 2.2:+47928571 MS.gene026086:CDS
AAAACTGTTGTCAACCTCTC+TGG 0.459812 2.2:+47928193 MS.gene026086:CDS
GGATTACACGATTTGATTCA+CGG 0.464996 2.2:-47926804 None:intergenic
GGCAGGGTCCTTACAGTTCA+CGG 0.470147 2.2:+47928976 MS.gene026086:intron
CCTTGTTGCTGCATTGGAAA+AGG 0.484553 2.2:+47926173 MS.gene026086:CDS
CTCCCCAATAGTTACCATAC+TGG 0.488293 2.2:-47926747 None:intergenic
AACAAGGTTCAACATGAGAT+TGG 0.492861 2.2:-47926157 None:intergenic
CAATAAGGGTACTGTTAGCT+AGG 0.496770 2.2:+47929161 MS.gene026086:CDS
GAAACCATTGACCACAATAA+GGG 0.497271 2.2:+47929147 MS.gene026086:CDS
TACTATGTCCAACAATTGCA+CGG 0.505039 2.2:-47926825 None:intergenic
GACATTGTTATCTTGTGTCA+TGG 0.505179 2.2:+47926028 MS.gene026086:CDS
CAGAAGAACCGTGAACTGTA+AGG 0.532860 2.2:-47928984 None:intergenic
GCAATTGTTGGACATAGTAA+AGG 0.535149 2.2:+47926829 MS.gene026086:CDS
AAACTCGTGTGCACTTTGAA+CGG 0.545147 2.2:-47929019 None:intergenic
TTGGAAAGAATTACGAGTGA+CGG 0.554529 2.2:+47928265 MS.gene026086:CDS
GGACGTTATGATCTGAAAGC+AGG 0.560803 2.2:+47928214 MS.gene026086:CDS
ATGGATTCTACAATCATGAC+AGG 0.573627 2.2:+47925838 MS.gene026086:CDS
TTTCCAAATAATCTTTACCA+AGG 0.575682 2.2:-47928249 None:intergenic
CATTTCCAGAAAAGTCAAAA+CGG 0.584654 2.2:-47926212 None:intergenic
GCAGGCATCGAAGAACGCCT+TGG 0.585105 2.2:+47928232 MS.gene026086:CDS
TTCCAGTATGGTAACTATTG+GGG 0.594109 2.2:+47926745 MS.gene026086:CDS
TGCAATCTTTGTGAATCTGA+AGG 0.602030 2.2:-47928637 None:intergenic
TGGTAACTATTGGGGAGAGG+TGG 0.607132 2.2:+47926753 MS.gene026086:CDS
GTATGGTAACTATTGGGGAG+AGG 0.614685 2.2:+47926750 MS.gene026086:CDS
AGCTAACAGTACCCTTATTG+TGG 0.617553 2.2:-47929158 None:intergenic
TTAAACTTTCCTTAGTAACA+CGG 0.641846 2.2:-47928580 None:intergenic
ACTCGTGTGCACTTTGAACG+GGG 0.654979 2.2:-47929017 None:intergenic
TTACCTTCCAGGGAAAGCGA+AGG 0.669336 2.2:+47926718 MS.gene026086:intron
TCAGATCATAACGTCCAGAG+AGG 0.688785 2.2:-47928207 None:intergenic
GATGAAGCTCATAACAACTG+AGG 0.696495 2.2:-47929121 None:intergenic
AGTTGTTATGAGCTTCATCG+AGG 0.706507 2.2:+47929125 MS.gene026086:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGATTAAAATTGATAAAA+TGG - chr2.2:47926343-47926362 None:intergenic 10.0%
!! AAACTATAGAATAAATGAAA+AGG - chr2.2:47926084-47926103 None:intergenic 15.0%
!! AGAAAATAGAATTATTGAAT+TGG + chr2.2:47926607-47926626 MS.gene026086:intron 15.0%
!! ATTAATCAATGAAAATAATC+TGG - chr2.2:47926116-47926135 None:intergenic 15.0%
!! CAATGAATGATTATATAAAT+TGG - chr2.2:47926928-47926947 None:intergenic 15.0%
!! CTATCTTAAAATGAAATATA+AGG - chr2.2:47927960-47927979 None:intergenic 15.0%
!! GAAAATAGAATTATTGAATT+GGG + chr2.2:47926608-47926627 MS.gene026086:intron 15.0%
!!! ATATTCAATTTTACATTGTT+TGG + chr2.2:47926278-47926297 MS.gene026086:intron 15.0%
!! AAAATTGAATATTAACTCTC+TGG - chr2.2:47926270-47926289 None:intergenic 20.0%
!! GATAATTCTTTGTCAATTTA+TGG + chr2.2:47927366-47927385 MS.gene026086:intron 20.0%
!! TCTTCATATTGCATTATTTA+AGG - chr2.2:47927779-47927798 None:intergenic 20.0%
!!! AAATTTTCAACGTAATCAAA+GGG + chr2.2:47927306-47927325 MS.gene026086:intron 20.0%
!!! AGAATTCAGATAGATTTTAA+CGG + chr2.2:47928422-47928441 MS.gene026086:intron 20.0%
!!! AGTTTTAATGTCATGATATT+TGG - chr2.2:47928179-47928198 None:intergenic 20.0%
!!! TGTATATCTAGATATGTTTT+TGG + chr2.2:47926885-47926904 MS.gene026086:intron 20.0%
!!! TTCTAGTCTTTTAAAAAATG+TGG - chr2.2:47927465-47927484 None:intergenic 20.0%
! AAATTACATCATATATGGCA+GGG + chr2.2:47928960-47928979 MS.gene026086:intron 25.0%
! AAGAATTTGAGAAGATTGAT+TGG - chr2.2:47928062-47928081 None:intergenic 25.0%
! AATAGAATTATTGAATTGGG+TGG + chr2.2:47926611-47926630 MS.gene026086:intron 25.0%
! ATAAGGATCAAATTCATATC+TGG - chr2.2:47927943-47927962 None:intergenic 25.0%
! ATGCCAAATTACATCATATA+TGG + chr2.2:47928955-47928974 MS.gene026086:intron 25.0%
! ATGCTTGAACAACAAATAAA+TGG - chr2.2:47928708-47928727 None:intergenic 25.0%
! ATGTATATTGTGGTGTATAA+TGG - chr2.2:47926959-47926978 None:intergenic 25.0%
! GAACTATACTACAAATTGAT+CGG + chr2.2:47926391-47926410 MS.gene026086:intron 25.0%
! TCTTCAAAAATTGCACTTAA+TGG - chr2.2:47928858-47928877 None:intergenic 25.0%
! TCTTGTAATATTATAAGCCT+GGG - chr2.2:47928329-47928348 None:intergenic 25.0%
! TGTTACTAAGGAAAGTTTAA+TGG + chr2.2:47928583-47928602 MS.gene026086:CDS 25.0%
! TTAAACTTTCCTTAGTAACA+CGG - chr2.2:47928583-47928602 None:intergenic 25.0%
! TTTCCAAATAATCTTTACCA+AGG - chr2.2:47928252-47928271 None:intergenic 25.0%
!! GAAATTTTCAACGTAATCAA+AGG + chr2.2:47927305-47927324 MS.gene026086:intron 25.0%
!! GAATGTGTTTTACTTCAATT+TGG - chr2.2:47927018-47927037 None:intergenic 25.0%
!!! GAATTCAGATAGATTTTAAC+GGG + chr2.2:47928423-47928442 MS.gene026086:intron 25.0%
!!! GATAGTTGTTACTTTTTAAC+TGG + chr2.2:47927975-47927994 MS.gene026086:intron 25.0%
AAAAACGCTGAAAAACTTGT+TGG + chr2.2:47925977-47925996 MS.gene026086:CDS 30.0%
AAGGATATGTTTCATGAAGT+GGG - chr2.2:47927560-47927579 None:intergenic 30.0%
AATAAGTTCATCAACACTAC+AGG - chr2.2:47928908-47928927 None:intergenic 30.0%
ACAATTATGTTCAGTGCATT+TGG - chr2.2:47927334-47927353 None:intergenic 30.0%
AGGTTTATTGTATTGCAGAA+AGG - chr2.2:47927047-47927066 None:intergenic 30.0%
ATAAATGGACCAGAAGTAAA+AGG - chr2.2:47928693-47928712 None:intergenic 30.0%
ATGATATGCAACTTGTGATT+TGG - chr2.2:47929057-47929076 None:intergenic 30.0%
ATTGTTTGGATGTATAGAGA+TGG + chr2.2:47926292-47926311 MS.gene026086:intron 30.0%
CAAATTACATCATATATGGC+AGG + chr2.2:47928959-47928978 MS.gene026086:intron 30.0%
CAATGAAAATAATCTGGCAA+AGG - chr2.2:47926110-47926129 None:intergenic 30.0%
CATTTCCAGAAAAGTCAAAA+CGG - chr2.2:47926215-47926234 None:intergenic 30.0%
CTCTTGTAATATTATAAGCC+TGG - chr2.2:47928330-47928349 None:intergenic 30.0%
CTGCCATATATGATGTAATT+TGG - chr2.2:47928961-47928980 None:intergenic 30.0%
GAAAGCAAACAATAAAGACA+AGG - chr2.2:47926677-47926696 None:intergenic 30.0%
GGATTTCAGAAAGACTTAAT+TGG - chr2.2:47927444-47927463 None:intergenic 30.0%
GGCTAAGCTTAAAAAAAGTA+TGG - chr2.2:47928837-47928856 None:intergenic 30.0%
GTTAAATGAAAGCTCATTTG+TGG - chr2.2:47927833-47927852 None:intergenic 30.0%
TAAGGATATGTTTCATGAAG+TGG - chr2.2:47927561-47927580 None:intergenic 30.0%
TAATCTTAACATCCAAAGCA+GGG - chr2.2:47927753-47927772 None:intergenic 30.0%
TACGTTGAAAATTTCAGTGA+TGG - chr2.2:47927300-47927319 None:intergenic 30.0%
TCAGAAAGACTTAATTGGAT+AGG - chr2.2:47927439-47927458 None:intergenic 30.0%
TGAAAATTTCAGTGATGGTT+TGG - chr2.2:47927295-47927314 None:intergenic 30.0%
! AACAGTTCCTGTCATAATTT+TGG - chr2.2:47927706-47927725 None:intergenic 30.0%
! ACTTATTGACTACTAAACTC+AGG - chr2.2:47927893-47927912 None:intergenic 30.0%
! TAAATTTTCATTATCCGCGA+TGG - chr2.2:47928457-47928476 None:intergenic 30.0%
! TTAATTGGATAGGTGGATTT+GGG - chr2.2:47927429-47927448 None:intergenic 30.0%
!! ACAAGTTTTGGTAGGAAATT+AGG - chr2.2:47927868-47927887 None:intergenic 30.0%
!! AGTTTTTCAGCGTTTTTGTT+CGG - chr2.2:47925974-47925993 None:intergenic 30.0%
!!! GTTTTGACTTTTCTGGAAAT+GGG + chr2.2:47926214-47926233 MS.gene026086:CDS 30.0%
AACAAGGTTCAACATGAGAT+TGG - chr2.2:47926160-47926179 None:intergenic 35.0%
ACGCCTTGGTAAAGATTATT+TGG + chr2.2:47928246-47928265 MS.gene026086:CDS 35.0%
ACTTTGAACTTGTGTTCGTT+TGG + chr2.2:47926512-47926531 MS.gene026086:intron 35.0%
AGCTAGGAACATGTATATTG+TGG - chr2.2:47926969-47926988 None:intergenic 35.0%
AGCTAGGGAGAGAAAAATTA+CGG - chr2.2:47927585-47927604 None:intergenic 35.0%
AGGATATGTTTCATGAAGTG+GGG - chr2.2:47927559-47927578 None:intergenic 35.0%
ATGGATTCTACAATCATGAC+AGG + chr2.2:47925838-47925857 MS.gene026086:CDS 35.0%
ATTCACAAAGATTGCAGGTT+AGG + chr2.2:47928644-47928663 MS.gene026086:intron 35.0%
CAACCAAAGTCAATCATTCT+CGG - chr2.2:47927250-47927269 None:intergenic 35.0%
GAAACCATTGACCACAATAA+GGG + chr2.2:47929147-47929166 MS.gene026086:CDS 35.0%
GAAAGACTTAATTGGATAGG+TGG - chr2.2:47927436-47927455 None:intergenic 35.0%
GAAGAGAGTTGAGTAATTTC+TGG + chr2.2:47927794-47927813 MS.gene026086:intron 35.0%
GACATTGTTATCTTGTGTCA+TGG + chr2.2:47926028-47926047 MS.gene026086:CDS 35.0%
GATCAAATTCATATCTGGCA+CGG - chr2.2:47927938-47927957 None:intergenic 35.0%
GCAAATTTGTCTAGACTGAT+TGG + chr2.2:47928094-47928113 MS.gene026086:intron 35.0%
GCAATTGTTGGACATAGTAA+AGG + chr2.2:47926829-47926848 MS.gene026086:CDS 35.0%
GGAGAGAAAAATTACGGTTA+AGG - chr2.2:47927579-47927598 None:intergenic 35.0%
GGATTACACGATTTGATTCA+CGG - chr2.2:47926807-47926826 None:intergenic 35.0%
GTAATCTTAACATCCAAAGC+AGG - chr2.2:47927754-47927773 None:intergenic 35.0%
GTTCCAGTATGGTAACTATT+GGG + chr2.2:47926744-47926763 MS.gene026086:CDS 35.0%
TAAACCCACAAGCTAAAGTT+AGG - chr2.2:47926701-47926720 None:intergenic 35.0%
TACTATGTCCAACAATTGCA+CGG - chr2.2:47926828-47926847 None:intergenic 35.0%
TCTAGACAAATTTGCGTGAA+AGG - chr2.2:47928089-47928108 None:intergenic 35.0%
TGCAATCTTTGTGAATCTGA+AGG - chr2.2:47928640-47928659 None:intergenic 35.0%
TTCAGATTCACAAAGATTGC+AGG + chr2.2:47928639-47928658 MS.gene026086:CDS 35.0%
TTCCAGTATGGTAACTATTG+GGG + chr2.2:47926745-47926764 MS.gene026086:CDS 35.0%
TTGGAAAGAATTACGAGTGA+CGG + chr2.2:47928265-47928284 MS.gene026086:CDS 35.0%
! ATCTCTTCTCCTTTTACTTC+TGG + chr2.2:47928681-47928700 MS.gene026086:intron 35.0%
! CTTAATTGGATAGGTGGATT+TGG - chr2.2:47927430-47927449 None:intergenic 35.0%
! GAGCATTTAGAGCAATTTAC+AGG - chr2.2:47927067-47927086 None:intergenic 35.0%
! TAAACTCAGGCTACAAGTTT+TGG - chr2.2:47927880-47927899 None:intergenic 35.0%
! TTTGGATTACCGTGTTACTA+AGG + chr2.2:47928571-47928590 MS.gene026086:CDS 35.0%
!! ATTTGTTGTTCAAGCATGTG+TGG + chr2.2:47928711-47928730 MS.gene026086:intron 35.0%
!! TTCTCCTAACTTTAGCTTGT+GGG + chr2.2:47926694-47926713 MS.gene026086:intron 35.0%
!! TTTCTCCTAACTTTAGCTTG+TGG + chr2.2:47926693-47926712 MS.gene026086:intron 35.0%
!!! CGTTTTGACTTTTCTGGAAA+TGG + chr2.2:47926213-47926232 MS.gene026086:CDS 35.0%
!!! CTCTCATTTGTTTTAGTAGC+TGG + chr2.2:47925923-47925942 MS.gene026086:intron 35.0%
!!! GTTTGCAGCATCTCTTTTTT+TGG - chr2.2:47927169-47927188 None:intergenic 35.0%
!!! TCTTTCCGTTTTGACTTTTC+TGG + chr2.2:47926207-47926226 MS.gene026086:CDS 35.0%
AAAACTGTTGTCAACCTCTC+TGG + chr2.2:47928193-47928212 MS.gene026086:CDS 40.0%
AAACTCGTGTGCACTTTGAA+CGG - chr2.2:47929022-47929041 None:intergenic 40.0%
ACTACAGGTAGGTATGGAAA+TGG - chr2.2:47928893-47928912 None:intergenic 40.0%
AGCTAACAGTACCCTTATTG+TGG - chr2.2:47929161-47929180 None:intergenic 40.0%
AGTACCCTTATTGTGGTCAA+TGG - chr2.2:47929154-47929173 None:intergenic 40.0%
AGTTCATCAACACTACAGGT+AGG - chr2.2:47928904-47928923 None:intergenic 40.0%
AGTTGTTATGAGCTTCATCG+AGG + chr2.2:47929125-47929144 MS.gene026086:CDS 40.0%
ATCAACACTACAGGTAGGTA+TGG - chr2.2:47928899-47928918 None:intergenic 40.0%
ATCTACTGTTGCAGGAAGTT+TGG + chr2.2:47928553-47928572 MS.gene026086:intron 40.0%
ATTGATGTGAAGACGAAGTC+AGG + chr2.2:47928292-47928311 MS.gene026086:CDS 40.0%
ATTTCATGTCAACCCTGCTT+TGG + chr2.2:47927738-47927757 MS.gene026086:intron 40.0%
CCATGCACCAAAATTATGAC+AGG + chr2.2:47927696-47927715 MS.gene026086:intron 40.0%
CGTTCCAGTATGGTAACTAT+TGG + chr2.2:47926743-47926762 MS.gene026086:CDS 40.0%
CTACAGGTAGGTATGGAAAT+GGG - chr2.2:47928892-47928911 None:intergenic 40.0%
GATATGTTTCATGAAGTGGG+GGG - chr2.2:47927557-47927576 None:intergenic 40.0%
GATGAAGCTCATAACAACTG+AGG - chr2.2:47929124-47929143 None:intergenic 40.0%
GGAAACCATTGACCACAATA+AGG + chr2.2:47929146-47929165 MS.gene026086:CDS 40.0%
GGAGAAGAGAGTTATTCCAA+TGG - chr2.2:47927511-47927530 None:intergenic 40.0%
GGATATGTTTCATGAAGTGG+GGG - chr2.2:47927558-47927577 None:intergenic 40.0%
GTCAATGTGAATGTCAACAC+AGG - chr2.2:47926568-47926587 None:intergenic 40.0%
GTCAGGTAAATTGTTTACCC+AGG + chr2.2:47928309-47928328 MS.gene026086:intron 40.0%
GTTGGCATATTGCATGAATC+TGG + chr2.2:47925995-47926014 MS.gene026086:CDS 40.0%
TCAGAACTCAGAAGTTACAC+TGG - chr2.2:47928039-47928058 None:intergenic 40.0%
TCCACACAAATAACGTGACA+GGG + chr2.2:47927140-47927159 MS.gene026086:intron 40.0%
TGTGGTCTCTATGAAAAGCT+AGG - chr2.2:47926985-47927004 None:intergenic 40.0%
TTCATATCTGGCACGGTTTA+AGG - chr2.2:47927931-47927950 None:intergenic 40.0%
TTGAGAACATCTACTGTTGC+AGG + chr2.2:47928545-47928564 MS.gene026086:intron 40.0%
TTGTGTGGAGTTCAATCTTG+CGG - chr2.2:47927129-47927148 None:intergenic 40.0%
! CGAGAATGATTGACTTTGGT+TGG + chr2.2:47927248-47927267 MS.gene026086:intron 40.0%
! CTGGTAGCAACACTCTTTTT+GGG - chr2.2:47927663-47927682 None:intergenic 40.0%
!! CAGATAGATTTTAACGGGCA+AGG + chr2.2:47928428-47928447 MS.gene026086:intron 40.0%
!! CCTGTCATAATTTTGGTGCA+TGG - chr2.2:47927699-47927718 None:intergenic 40.0%
!! TTTTGGTGCATGGAGAAAAG+CGG - chr2.2:47927689-47927708 None:intergenic 40.0%
AACTCGTGTGCACTTTGAAC+GGG - chr2.2:47929021-47929040 None:intergenic 45.0%
ACCCTGTCACGTTATTTGTG+TGG - chr2.2:47927144-47927163 None:intergenic 45.0%
CACAAAGATTGCAGGTTAGG+TGG + chr2.2:47928647-47928666 MS.gene026086:intron 45.0%
CAGAAGAACCGTGAACTGTA+AGG - chr2.2:47928987-47929006 None:intergenic 45.0%
CCAAAGTCAATCATTCTCGG+AGG - chr2.2:47927247-47927266 None:intergenic 45.0%
CCTTGTTGCTGCATTGGAAA+AGG + chr2.2:47926173-47926192 MS.gene026086:CDS 45.0%
CGTGTAATCCGTGCAATTGT+TGG + chr2.2:47926817-47926836 MS.gene026086:CDS 45.0%
CTCCACACAAATAACGTGAC+AGG + chr2.2:47927139-47927158 MS.gene026086:intron 45.0%
CTCCCCAATAGTTACCATAC+TGG - chr2.2:47926750-47926769 None:intergenic 45.0%
GGACGTTATGATCTGAAAGC+AGG + chr2.2:47928214-47928233 MS.gene026086:CDS 45.0%
GTATGGTAACTATTGGGGAG+AGG + chr2.2:47926750-47926769 MS.gene026086:CDS 45.0%
GTTGAACCTTGTTGCTGCAT+TGG + chr2.2:47926167-47926186 MS.gene026086:CDS 45.0%
TCAGATCATAACGTCCAGAG+AGG - chr2.2:47928210-47928229 None:intergenic 45.0%
TGAACCATGCCATCTATGTC+CGG + chr2.2:47926439-47926458 MS.gene026086:intron 45.0%
! ATAGGTGGATTTGGGTGGAT+GGG - chr2.2:47927421-47927440 None:intergenic 45.0%
! ATGTTTTCGTGTCGTGATGC+AGG + chr2.2:47928127-47928146 MS.gene026086:intron 45.0%
! ATTGGATAGGTGGATTTGGG+TGG - chr2.2:47927426-47927445 None:intergenic 45.0%
! CCTCCGAGAATGATTGACTT+TGG + chr2.2:47927244-47927263 MS.gene026086:intron 45.0%
! CCTTTTCCAATGCAGCAACA+AGG - chr2.2:47926176-47926195 None:intergenic 45.0%
! GCTGGTAGCAACACTCTTTT+TGG - chr2.2:47927664-47927683 None:intergenic 45.0%
! TCAACAGCTTTTGCAGCTGA+AGG - chr2.2:47927094-47927113 None:intergenic 45.0%
!! ACACTCAGTCGTTTTGCCAT+TGG + chr2.2:47927492-47927511 MS.gene026086:intron 45.0%
!! CAATTTGGTTTGCGAGAGTG+TGG - chr2.2:47927003-47927022 None:intergenic 45.0%
!! CTCAGGCTACAAGTTTTGGT+AGG - chr2.2:47927876-47927895 None:intergenic 45.0%
!! GTAGCAACACTCTTTTTGGG+AGG - chr2.2:47927660-47927679 None:intergenic 45.0%
!!! TTAATTTTTTTTTGTATATT+AGG + chr2.2:47926131-47926150 MS.gene026086:intron 5.0%
ACTCGTGTGCACTTTGAACG+GGG - chr2.2:47929020-47929039 None:intergenic 50.0%
AGCGAAGGTTCGTTCCAGTA+TGG + chr2.2:47926733-47926752 MS.gene026086:CDS 50.0%
AGCTTGTGGGTTTACCTTCC+AGG + chr2.2:47926707-47926726 MS.gene026086:intron 50.0%
AGTTCAATCTTGCGGTGGCT+CGG - chr2.2:47927121-47927140 None:intergenic 50.0%
ATCCTTCCAAACGAGCAACC+CGG - chr2.2:47926461-47926480 None:intergenic 50.0%
CCCTAGCTACTATGTACAGC+CGG + chr2.2:47927597-47927616 MS.gene026086:intron 50.0%
CCGGCTGTACATAGTAGCTA+GGG - chr2.2:47927600-47927619 None:intergenic 50.0%
GAACCATGCCATCTATGTCC+GGG + chr2.2:47926440-47926459 MS.gene026086:intron 50.0%
GCTTGTGGGTTTACCTTCCA+GGG + chr2.2:47926708-47926727 MS.gene026086:intron 50.0%
TGCTACACGGGGATCACATT+CGG - chr2.2:47927220-47927239 None:intergenic 50.0%
TGGTAACTATTGGGGAGAGG+TGG + chr2.2:47926753-47926772 MS.gene026086:CDS 50.0%
TGTGGAGTTCAATCTTGCGG+TGG - chr2.2:47927126-47927145 None:intergenic 50.0%
TGTTTCATGAAGTGGGGGGT+CGG - chr2.2:47927553-47927572 None:intergenic 50.0%
TTACCTTCCAGGGAAAGCGA+AGG + chr2.2:47926718-47926737 MS.gene026086:intron 50.0%
TTCCTCTTCCCCACTGAAGA+AGG + chr2.2:47927190-47927209 MS.gene026086:intron 50.0%
! AGCTTTTGCAGCTGAAGGTC+GGG - chr2.2:47927089-47927108 None:intergenic 50.0%
! CAGCTTTTGCAGCTGAAGGT+CGG - chr2.2:47927090-47927109 None:intergenic 50.0%
! GATAGGTGGATTTGGGTGGA+TGG - chr2.2:47927422-47927441 None:intergenic 50.0%
! TTCGGTGTGCCTTCTTCAGT+GGG - chr2.2:47927202-47927221 None:intergenic 50.0%
! TTTTCGTGTCGTGATGCAGG+AGG + chr2.2:47928130-47928149 MS.gene026086:intron 50.0%
!! ACTCTTTTTGGGAGGACCCA+TGG - chr2.2:47927652-47927671 None:intergenic 50.0%
!! ATTCGGTGTGCCTTCTTCAG+TGG - chr2.2:47927203-47927222 None:intergenic 50.0%
!! CACTGAAAGACGGTTTTGGC+CGG - chr2.2:47927619-47927638 None:intergenic 50.0%
!! CTCTTTTTGGGAGGACCCAT+GGG - chr2.2:47927651-47927670 None:intergenic 50.0%
!! GATGGTGGAGCACTGAAAGA+CGG - chr2.2:47927629-47927648 None:intergenic 50.0%
!!! GGAGCACTGAAAGACGGTTT+TGG - chr2.2:47927623-47927642 None:intergenic 50.0%
AACGGGCAAGGTTTCCATCG+CGG + chr2.2:47928440-47928459 MS.gene026086:intron 55.0%
ACGAGCAACCCGGACATAGA+TGG - chr2.2:47926451-47926470 None:intergenic 55.0%
CAACCCGGACATAGATGGCA+TGG - chr2.2:47926446-47926465 None:intergenic 55.0%
CTATGTCCGGGTTGCTCGTT+TGG + chr2.2:47926452-47926471 MS.gene026086:intron 55.0%
GAACCTTCGCTTTCCCTGGA+AGG - chr2.2:47926724-47926743 None:intergenic 55.0%
GAACGAACCTTCGCTTTCCC+TGG - chr2.2:47926728-47926747 None:intergenic 55.0%
GCCGGCTGTACATAGTAGCT+AGG - chr2.2:47927601-47927620 None:intergenic 55.0%
GGCAGGGTCCTTACAGTTCA+CGG + chr2.2:47928976-47928995 MS.gene026086:intron 55.0%
GTGGATTTGGGTGGATGGGA+GGG - chr2.2:47927417-47927436 None:intergenic 55.0%
TCAGTGCTCCACCATCCCAT+GGG + chr2.2:47927633-47927652 MS.gene026086:intron 55.0%
TCGGTGTGCCTTCTTCAGTG+GGG - chr2.2:47927201-47927220 None:intergenic 55.0%
TCTCGGAGGAACGTGCTACA+CGG - chr2.2:47927233-47927252 None:intergenic 55.0%
TGCCTTCTTCAGTGGGGAAG+AGG - chr2.2:47927195-47927214 None:intergenic 55.0%
TGGATTTGGGTGGATGGGAG+GGG - chr2.2:47927416-47927435 None:intergenic 55.0%
TTCAGTGCTCCACCATCCCA+TGG + chr2.2:47927632-47927651 MS.gene026086:intron 55.0%
!! TTTTGGGAGGACCCATGGGA+TGG - chr2.2:47927647-47927666 None:intergenic 55.0%
CTCGGAGGAACGTGCTACAC+GGG - chr2.2:47927232-47927251 None:intergenic 60.0%
GATTTGGGTGGATGGGAGGG+GGG - chr2.2:47927414-47927433 None:intergenic 60.0%
GCATGGAGAAAAGCGGCTGC+TGG - chr2.2:47927682-47927701 None:intergenic 60.0%
GGATTTGGGTGGATGGGAGG+GGG - chr2.2:47927415-47927434 None:intergenic 60.0%
GTCCGGGTTGCTCGTTTGGA+AGG + chr2.2:47926456-47926475 MS.gene026086:intron 60.0%
TCGGAGGAACGTGCTACACG+GGG - chr2.2:47927231-47927250 None:intergenic 60.0%
! GGAGAGTGTGGCTGTGGGAT+CGG - chr2.2:47927532-47927551 None:intergenic 60.0%
! GGTGGATTTGGGTGGATGGG+AGG - chr2.2:47927418-47927437 None:intergenic 60.0%
!! GCAGGCATCGAAGAACGCCT+TGG + chr2.2:47928232-47928251 MS.gene026086:CDS 60.0%
AAGTGGGGGGTCGGAGAGTG+TGG - chr2.2:47927544-47927563 None:intergenic 65.0%
TGGGAGGACCCATGGGATGG+TGG - chr2.2:47927644-47927663 None:intergenic 65.0%
! GGGTCGGAGAGTGTGGCTGT+GGG - chr2.2:47927537-47927556 None:intergenic 65.0%
! GGGGTCGGAGAGTGTGGCTG+TGG - chr2.2:47927538-47927557 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 47925838 47929182 47925838 ID=MS.gene026086
chr2.2 mRNA 47925838 47929182 47925838 ID=MS.gene026086.t1;Parent=MS.gene026086
chr2.2 exon 47925838 47925859 47925838 ID=MS.gene026086.t1.exon1;Parent=MS.gene026086.t1
chr2.2 CDS 47925838 47925859 47925838 ID=cds.MS.gene026086.t1;Parent=MS.gene026086.t1
chr2.2 exon 47925942 47926066 47925942 ID=MS.gene026086.t1.exon2;Parent=MS.gene026086.t1
chr2.2 CDS 47925942 47926066 47925942 ID=cds.MS.gene026086.t1;Parent=MS.gene026086.t1
chr2.2 exon 47926153 47926235 47926153 ID=MS.gene026086.t1.exon3;Parent=MS.gene026086.t1
chr2.2 CDS 47926153 47926235 47926153 ID=cds.MS.gene026086.t1;Parent=MS.gene026086.t1
chr2.2 exon 47926729 47926850 47926729 ID=MS.gene026086.t1.exon4;Parent=MS.gene026086.t1
chr2.2 CDS 47926729 47926850 47926729 ID=cds.MS.gene026086.t1;Parent=MS.gene026086.t1
chr2.2 exon 47928149 47928313 47928149 ID=MS.gene026086.t1.exon5;Parent=MS.gene026086.t1
chr2.2 CDS 47928149 47928313 47928149 ID=cds.MS.gene026086.t1;Parent=MS.gene026086.t1
chr2.2 exon 47928567 47928660 47928567 ID=MS.gene026086.t1.exon6;Parent=MS.gene026086.t1
chr2.2 CDS 47928567 47928660 47928567 ID=cds.MS.gene026086.t1;Parent=MS.gene026086.t1
chr2.2 exon 47928981 47929182 47928981 ID=MS.gene026086.t1.exon7;Parent=MS.gene026086.t1
chr2.2 CDS 47928981 47929182 47928981 ID=cds.MS.gene026086.t1;Parent=MS.gene026086.t1
Gene Sequence

>MS.gene026086

ATGGATTCTACAATCATGACAGGTATATTTACATTCTTGTTTGATATTACTTGTGATATATTGTATCTGTCTATGTTTACGTAATCTCTCATTTGTTTTAGTAGCTGGTCAGCGTCATCAGAGAGTTATAATACCGAACAAAAACGCTGAAAAACTTGTTGGCATATTGCATGAATCTGGAACAACGAACGACATTGTTATCTTGTGTCATGGTTTTCGTTGCACTAAAGTTTGTTACTTTTTCCTTTTCATTTATTCTATAGTTTGTTCCTTTGCCAGATTATTTTCATTGATTAATTTTTTTTTGTATATTAGGATACCAATCTCATGTTGAACCTTGTTGCTGCATTGGAAAAGGCACGAATTAGTTCTTTCCGTTTTGACTTTTCTGGAAATGGGTATGTTTATTTATGATGATGCATAACACAACCAGAGAGTTAATATTCAATTTTACATTGTTTGGATGTATAGAGATGGATAATGCAATATGTGTTATTTGATTCCATTTTATCAATTTTAATCTATTCATATGCTGTCAAAATATTTGTAAATTGAACTATACTACAAATTGATCGGCTAAAAGCTGAAAAATATAAAAAAGTGAACCATGCCATCTATGTCCGGGTTGCTCGTTTGGAAGGATACATTTATTCTTAGCTTGTATGTAGTTTATGACTTTGAACTTGTGTTCGTTTGGTGTGCATGATTAGTATGATTAAAGCATATACCTGTGTTGACATTCACATTGACTCGTAGACACTAGTATTTGAGAAAATAGAATTATTGAATTGGGTGGTATTTTTCTTCATGTACACTGTAGTTTCTGAAGTCTGATACCTTGTCTTTATTGTTTGCTTTCTCCTAACTTTAGCTTGTGGGTTTACCTTCCAGGGAAAGCGAAGGTTCGTTCCAGTATGGTAACTATTGGGGAGAGGTGGATGAATTACACACTGTTGTTCAACATTTCCGTGAATCAAATCGTGTAATCCGTGCAATTGTTGGACATAGTAAAGGTAAGTTGATTTGTTTGCTTTTACGAAAAAAGAATGTATATCTAGATATGTTTTTGGATATGTGATTATATGTGCCAATTTATATAATCATTCATTGATCATTTTCCATTATACACCACAATATACATGTTCCTAGCTTTTCATAGAGACCACACTCTCGCAAACCAAATTGAAGTAAAACACATTCTGAATACCTTTCTGCAATACAATAAACCTGTAAATTGCTCTAAATGCTCCCGACCTTCAGCTGCAAAAGCTGTTGAAACACCGAGCCACCGCAAGATTGAACTCCACACAAATAACGTGACAGGGTAGCCAAAAAAAGAGATGCTGCAAACTTTCCTCTTCCCCACTGAAGAAGGCACACCGAATGTGATCCCCGTGTAGCACGTTCCTCCGAGAATGATTGACTTTGGTTGGAAGTCTATCTTGAAGTAATCTCCAAACCATCACTGAAATTTTCAACGTAATCAAAGGGTTCCAAATGCACTGAACATAATTGTTATTAATGATAAGATAATTCTTTGTCAATTTATGGCTCGCTTTCTTAACAGAATACGCCCCCCTCCCATCCACCCAAATCCACCTATCCAATTAAGTCTTTCTGAAATCCACATTTTTTAAAAGACTAGAACATTCTAACACTCAGTCGTTTTGCCATTGGAATAACTCTCTTCTCCGATCCCACAGCCACACTCTCCGACCCCCCACTTCATGAAACATATCCTTAACCGTAATTTTTCTCTCCCTAGCTACTATGTACAGCCGGCCAAAACCGTCTTTCAGTGCTCCACCATCCCATGGGTCCTCCCAAAAAGAGTGTTGCTACCAGCAGCCGCTTTTCTCCATGCACCAAAATTATGACAGGAACTGTTCTTGTCAATATAATTTCATGTCAACCCTGCTTTGGATGTTAAGATTACAACCTTAAATAATGCAATATGAAGAGAGTTGAGTAATTTCTGGAAGAAAAAAAAGACCACAAATGAGCTTTCATTTAACTGATGTGTAACACCTAATTTCCTACCAAAACTTGTAGCCTGAGTTTAGTAGTCAATAAGTTGAAATATAAAGAAACCTTAAACCGTGCCAGATATGAATTTGATCCTTATATTTCATTTTAAGATAGTTGTTACTTTTTAACTGGTACTATATTTTCATCTATAGTTCTTGAAAGTTGAATTGCCAGTGTAACTTCTGAGTTCTGACCAATCAATCTTCTCAAATTCTTATGTCCTTTCACGCAAATTTGTCTAGACTGATTGGACTAATAGTAATGTTTTCGTGTCGTGATGCAGGAGGAGACATAGTGCTTTTGTATGCTTCCAAATATCATGACATTAAAACTGTTGTCAACCTCTCTGGACGTTATGATCTGAAAGCAGGCATCGAAGAACGCCTTGGTAAAGATTATTTGGAAAGAATTACGAGTGACGGTTTTATTGATGTGAAGACGAAGTCAGGTAAATTGTTTACCCAGGCTTATAATATTACAAGAGCAAAGTTCGAGCTAGACATAATCGTGAACTATCTTGTTCAATAACTGCAAAATTACTTGAGTTTAAAACTTTAGAATTCAGATAGATTTTAACGGGCAAGGTTTCCATCGCGGATAATGAAAATTTACAAGTTCTTTGTTTCAATCTATGTAACACATATGCACTGAATTACTATGTTATTTATTAACTGATCATTTGAGAACATCTACTGTTGCAGGAAGTTTGGATTACCGTGTTACTAAGGAAAGTTTAATGGATCGCATGAATACAAACATGCATGAAGTGTGCCTTCAGATTCACAAAGATTGCAGGTTAGGTGGAAAGCTGTTTGATCTCTTCTCCTTTTACTTCTGGTCCATTTATTTGTTGTTCAAGCATGTGTGGCTGTTAAAATATCATGAATATTTCTACAATAGAAAAAATAAAAAACATGATCACTCTCAATTCTTATTATTATAGCAGTAAAATTAAATTTACTACATTTCCATACTTTTTTTAAGCTTAGCCATTAAGTGCAATTTTTGAAGAAAATTTAACTTCCCATTTCCATACCTACCTGTAGTGTTGATGAACTTATTAAGTTACATTCATGCATAGATATGCTGATGCCAAATTACATCATATATGGCAGGGTCCTTACAGTTCACGGTTCTTCTGACAAAATTATCCCCGTTCAAAGTGCACACGAGTTTGCTAAGATCATACCAAATCACAAGTTGCATATCATTAAACGAGCTAATCATGCATACAGTAATCATCAAGACGTGTTATCCTCAGTTGTTATGAGCTTCATCGAGGAAACCATTGACCACAATAAGGGTACTGTTAGCTAG
Protein sequence

>MS.gene026086.t1

MDSTIMTAGQRHQRVIIPNKNAEKLVGILHESGTTNDIVILCHGFRCTKDTNLMLNLVAALEKARISSFRFDFSGNGESEGSFQYGNYWGEVDELHTVVQHFRESNRVIRAIVGHSKGGDIVLLYASKYHDIKTVVNLSGRYDLKAGIEERLGKDYLERITSDGFIDVKTKSGSLDYRVTKESLMDRMNTNMHEVCLQIHKDCRVLTVHGSSDKIIPVQSAHEFAKIIPNHKLHIIKRANHAYSNHQDVLSSVVMSFIEETIDHNKGTVS