AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025116

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025116.t1 MTR_2g039570 91.698 265 22 0 6 270 1 265 0.0 504
MS.gene025116.t1 MTR_2g039510 82.510 263 44 1 10 270 11 273 8.50e-164 455
MS.gene025116.t1 MTR_2g039480 76.046 263 61 1 10 270 7 269 1.18e-146 411
MS.gene025116.t1 MTR_2g039450 71.206 257 74 0 10 266 11 267 2.58e-133 379
MS.gene025116.t1 MTR_4g126050 69.349 261 78 2 8 268 64 322 1.35e-132 378
MS.gene025116.t1 MTR_4g126120 61.450 262 100 1 1 262 1 261 2.28e-124 355
MS.gene025116.t1 MTR_4g126120 63.492 252 91 1 11 262 20 270 1.29e-123 353
MS.gene025116.t1 MTR_1g016050 58.871 248 101 1 12 259 7 253 5.48e-106 309
MS.gene025116.t1 MTR_4g126050 70.455 176 50 2 8 183 64 237 5.92e-86 256
MS.gene025116.t1 MTR_1g016060 42.308 182 84 2 88 268 467 628 4.39e-33 127
MS.gene025116.t1 MTR_2g039550 57.143 77 9 1 1 77 18 70 3.83e-19 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025116.t1 AT3G47590 63.320 259 92 2 7 265 53 308 6.41e-120 345
MS.gene025116.t1 AT1G29840 60.377 265 102 2 1 265 50 311 4.15e-117 338
MS.gene025116.t1 AT3G47560 61.390 259 94 3 1 259 1 253 1.94e-114 330
MS.gene025116.t1 AT3G47560 61.390 259 94 3 1 259 37 289 5.62e-114 330
MS.gene025116.t1 AT5G11910 58.140 258 107 1 2 259 11 267 6.30e-111 322
MS.gene025116.t1 AT5G11910 58.140 258 107 1 2 259 29 285 1.12e-110 322
MS.gene025116.t1 AT3G47560 56.184 283 96 3 1 259 1 279 1.66e-110 321
MS.gene025116.t1 AT3G47560 55.594 286 94 4 1 259 1 280 5.36e-108 314
MS.gene025116.t1 AT3G47560 51.290 310 96 4 1 259 1 306 4.38e-104 305
MS.gene025116.t1 AT2G19550 54.475 257 107 6 11 262 2 253 3.97e-96 286
MS.gene025116.t1 AT3G47590 66.049 162 54 1 7 168 53 213 8.65e-76 230
MS.gene025116.t1 AT5G11910 59.603 151 60 1 2 152 11 160 2.86e-60 188
MS.gene025116.t1 AT5G11910 59.603 151 60 1 2 152 29 178 2.86e-60 189

Find 46 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCCTTGGTAAAGATTATT+TGG 0.161587 2.4:+50429417 MS.gene025116:CDS
TGTTACTAAGGAAAGTTTAA+TGG 0.281740 2.4:+50429754 MS.gene025116:CDS
ATCTACTGTTGCAGGAAGTT+TGG 0.288735 2.4:+50429724 MS.gene025116:intron
CGTTCCAGTATGGTAACTAT+TGG 0.315195 2.4:+50427914 MS.gene025116:CDS
GTTCCAGTATGGTAACTATT+GGG 0.346161 2.4:+50427915 MS.gene025116:CDS
CGTGTAATCCGTGCAATTGT+TGG 0.348094 2.4:+50427988 MS.gene025116:CDS
GGAAACCATTGACCACAATA+AGG 0.355010 2.4:+50430317 MS.gene025116:CDS
ATGATATGCAACTTGTGATT+TGG 0.362877 2.4:-50430225 None:intergenic
TTCAGATTCACAAAGATTGC+AGG 0.382227 2.4:+50429810 MS.gene025116:CDS
GTTGGCATATTGCATGAATC+TGG 0.383653 2.4:+50427163 MS.gene025116:CDS
AACTCGTGTGCACTTTGAAC+GGG 0.393008 2.4:-50430189 None:intergenic
GTTGAACCTTGTTGCTGCAT+TGG 0.422568 2.4:+50427338 MS.gene025116:CDS
AGTACCCTTATTGTGGTCAA+TGG 0.432514 2.4:-50430322 None:intergenic
ATTGATGTGAAGACGAAGTC+AGG 0.444727 2.4:+50429463 MS.gene025116:CDS
AGCGAAGGTTCGTTCCAGTA+TGG 0.446124 2.4:+50427904 MS.gene025116:CDS
TTTGGATTACCGTGTTACTA+AGG 0.456099 2.4:+50429742 MS.gene025116:CDS
AAAACTGTTGTCAACCTCTC+TGG 0.459812 2.4:+50429364 MS.gene025116:CDS
GGATTACACGATTTGATTCA+CGG 0.464996 2.4:-50427975 None:intergenic
GGCAGGGTCCTTACAGTTCA+CGG 0.470147 2.4:+50430147 MS.gene025116:intron
CCTTGTTGCTGCATTGGAAA+AGG 0.484553 2.4:+50427344 MS.gene025116:CDS
CTCCCCAATAGTTACCATAC+TGG 0.488293 2.4:-50427918 None:intergenic
AACAAGGTTCAACATGAGAT+TGG 0.492861 2.4:-50427328 None:intergenic
CAATAAGGGTACTGTTAGCT+AGG 0.496770 2.4:+50430332 MS.gene025116:CDS
GAAACCATTGACCACAATAA+GGG 0.497271 2.4:+50430318 MS.gene025116:CDS
TACTATGTCCAACAATTGCA+CGG 0.505039 2.4:-50427996 None:intergenic
GACATTGTTATCTTGTGTCA+TGG 0.505179 2.4:+50427196 MS.gene025116:CDS
CAGAAGAACCGTGAACTGTA+AGG 0.532860 2.4:-50430155 None:intergenic
GCAATTGTTGGACATAGTAA+AGG 0.535149 2.4:+50428000 MS.gene025116:CDS
AAACTCGTGTGCACTTTGAA+CGG 0.545147 2.4:-50430190 None:intergenic
TTGGAAAGAATTACGAGTGA+CGG 0.554529 2.4:+50429436 MS.gene025116:CDS
GGACGTTATGATCTGAAAGC+AGG 0.560803 2.4:+50429385 MS.gene025116:CDS
ATGGATTCTACAATCATGAC+AGG 0.573627 2.4:+50427006 MS.gene025116:CDS
TTTCCAAATAATCTTTACCA+AGG 0.575682 2.4:-50429420 None:intergenic
CATTTCCAGAAAAGTCAAAA+CGG 0.584654 2.4:-50427383 None:intergenic
GCAGGCATCGAAGAACGCCT+TGG 0.585105 2.4:+50429403 MS.gene025116:CDS
TTCCAGTATGGTAACTATTG+GGG 0.594109 2.4:+50427916 MS.gene025116:CDS
TGCAATCTTTGTGAATCTGA+AGG 0.602030 2.4:-50429808 None:intergenic
TGGTAACTATTGGGGAGAGG+TGG 0.607132 2.4:+50427924 MS.gene025116:CDS
GTATGGTAACTATTGGGGAG+AGG 0.614685 2.4:+50427921 MS.gene025116:CDS
AGCTAACAGTACCCTTATTG+TGG 0.617553 2.4:-50430329 None:intergenic
TTAAACTTTCCTTAGTAACA+CGG 0.641846 2.4:-50429751 None:intergenic
ACTCGTGTGCACTTTGAACG+GGG 0.654979 2.4:-50430188 None:intergenic
TTACCTTCCAGGGAAAGCGA+AGG 0.669336 2.4:+50427889 MS.gene025116:intron
TCAGATCATAACGTCCAGAG+AGG 0.688785 2.4:-50429378 None:intergenic
GATGAAGCTCATAACAACTG+AGG 0.696495 2.4:-50430292 None:intergenic
AGTTGTTATGAGCTTCATCG+AGG 0.706507 2.4:+50430296 MS.gene025116:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATAGAATTATTGAAT+TGG + chr2.4:50427778-50427797 MS.gene025116:intron 15.0%
!! ATAGATCAAAATTGATAAAA+TGG - chr2.4:50427514-50427533 None:intergenic 15.0%
!! ATTAATCAATGAAAATAATC+TGG - chr2.4:50427287-50427306 None:intergenic 15.0%
!! CAATGAATGATTATATAAAT+TGG - chr2.4:50428099-50428118 None:intergenic 15.0%
!! CTATAGAATAATAAATGAAA+AGG - chr2.4:50427252-50427271 None:intergenic 15.0%
!! CTATCTTAAAATGAAATATA+AGG - chr2.4:50429131-50429150 None:intergenic 15.0%
!! GAAAATAGAATTATTGAATT+GGG + chr2.4:50427779-50427798 MS.gene025116:intron 15.0%
!!! ATATTCAATTTTACATTGTT+TGG + chr2.4:50427449-50427468 MS.gene025116:intron 15.0%
!! AAAATTGAATATTAACTCTC+TGG - chr2.4:50427441-50427460 None:intergenic 20.0%
!! GATAATTCTTTGTCAATTTA+TGG + chr2.4:50428537-50428556 MS.gene025116:intron 20.0%
!! TCTTCATATTGCATTATTTA+AGG - chr2.4:50428950-50428969 None:intergenic 20.0%
!!! AAATTTTCAACGTAATCAAA+GGG + chr2.4:50428477-50428496 MS.gene025116:intron 20.0%
!!! AGAATTCAGATAGATTTTAA+CGG + chr2.4:50429593-50429612 MS.gene025116:intron 20.0%
!!! AGTTTTAATGTCATGATATT+TGG - chr2.4:50429350-50429369 None:intergenic 20.0%
!!! TGTATATCTAGATATGTTTT+TGG + chr2.4:50428056-50428075 MS.gene025116:intron 20.0%
!!! TTCTAGTCTTTTAAAAAATG+TGG - chr2.4:50428636-50428655 None:intergenic 20.0%
! AAATTACATCATATATGGCA+GGG + chr2.4:50430131-50430150 MS.gene025116:intron 25.0%
! AAGAATTTGAGAAGATTGAT+TGG - chr2.4:50429233-50429252 None:intergenic 25.0%
! AATAGAATTATTGAATTGGG+TGG + chr2.4:50427782-50427801 MS.gene025116:intron 25.0%
! ATAAGGATCAAATTCATATC+TGG - chr2.4:50429114-50429133 None:intergenic 25.0%
! ATGCCAAATTACATCATATA+TGG + chr2.4:50430126-50430145 MS.gene025116:intron 25.0%
! ATGCTTGAACAACAAATAAA+TGG - chr2.4:50429879-50429898 None:intergenic 25.0%
! ATGTATATTGTGGTGTATAA+TGG - chr2.4:50428130-50428149 None:intergenic 25.0%
! GAACTATACTACAAATTGAT+CGG + chr2.4:50427562-50427581 MS.gene025116:intron 25.0%
! TCTTCAAAAATTGCACTTAA+TGG - chr2.4:50430029-50430048 None:intergenic 25.0%
! TCTTGTAATATTATAAGCCT+GGG - chr2.4:50429500-50429519 None:intergenic 25.0%
! TGTTACTAAGGAAAGTTTAA+TGG + chr2.4:50429754-50429773 MS.gene025116:CDS 25.0%
! TTAAACTTTCCTTAGTAACA+CGG - chr2.4:50429754-50429773 None:intergenic 25.0%
! TTTCCAAATAATCTTTACCA+AGG - chr2.4:50429423-50429442 None:intergenic 25.0%
!! GAAATTTTCAACGTAATCAA+AGG + chr2.4:50428476-50428495 MS.gene025116:intron 25.0%
!! GAATGTGTTTTACTTCAATT+TGG - chr2.4:50428189-50428208 None:intergenic 25.0%
!!! GAATTCAGATAGATTTTAAC+GGG + chr2.4:50429594-50429613 MS.gene025116:intron 25.0%
!!! GATAGTTGTTACTTTTTAAC+TGG + chr2.4:50429146-50429165 MS.gene025116:intron 25.0%
AAAAACGCTGAAAAACTTGT+TGG + chr2.4:50427145-50427164 MS.gene025116:CDS 30.0%
AAGGATATGTTTCATGAAGT+GGG - chr2.4:50428731-50428750 None:intergenic 30.0%
AATAAGTTCATCAACACTAC+AGG - chr2.4:50430079-50430098 None:intergenic 30.0%
ACAATTATGTTCAGTGCATT+TGG - chr2.4:50428505-50428524 None:intergenic 30.0%
AGGTTTATTGTATTGCAGAA+AGG - chr2.4:50428218-50428237 None:intergenic 30.0%
ATAAATGGACCAGAAGTAAA+AGG - chr2.4:50429864-50429883 None:intergenic 30.0%
ATGATATGCAACTTGTGATT+TGG - chr2.4:50430228-50430247 None:intergenic 30.0%
ATTGTTTGGATGTATAGAGA+TGG + chr2.4:50427463-50427482 MS.gene025116:intron 30.0%
CAAATTACATCATATATGGC+AGG + chr2.4:50430130-50430149 MS.gene025116:intron 30.0%
CAATGAAAATAATCTGGCAA+AGG - chr2.4:50427281-50427300 None:intergenic 30.0%
CATTTCCAGAAAAGTCAAAA+CGG - chr2.4:50427386-50427405 None:intergenic 30.0%
CTCTTGTAATATTATAAGCC+TGG - chr2.4:50429501-50429520 None:intergenic 30.0%
CTGCCATATATGATGTAATT+TGG - chr2.4:50430132-50430151 None:intergenic 30.0%
GAAAGCAAACAATAAAGACA+AGG - chr2.4:50427848-50427867 None:intergenic 30.0%
GGATTTCAGAAAGACTTAAT+TGG - chr2.4:50428615-50428634 None:intergenic 30.0%
GGCTAAGCTTAAAAAAAGTA+TGG - chr2.4:50430008-50430027 None:intergenic 30.0%
GTTAAATGAAAGCTCATTTG+TGG - chr2.4:50429004-50429023 None:intergenic 30.0%
TAAGGATATGTTTCATGAAG+TGG - chr2.4:50428732-50428751 None:intergenic 30.0%
TAATCTTAACATCCAAAGCA+GGG - chr2.4:50428924-50428943 None:intergenic 30.0%
TACGTTGAAAATTTCAGTGA+TGG - chr2.4:50428471-50428490 None:intergenic 30.0%
TCAGAAAGACTTAATTGGAT+AGG - chr2.4:50428610-50428629 None:intergenic 30.0%
TGAAAATTTCAGTGATGGTT+TGG - chr2.4:50428466-50428485 None:intergenic 30.0%
! AACAGTTCCTGTCATAATTT+TGG - chr2.4:50428877-50428896 None:intergenic 30.0%
! ACTTATTGACTACTAAACTC+AGG - chr2.4:50429064-50429083 None:intergenic 30.0%
! TAAATTTTCATTATCCGCGA+TGG - chr2.4:50429628-50429647 None:intergenic 30.0%
! TTAATTGGATAGGTGGATTT+GGG - chr2.4:50428600-50428619 None:intergenic 30.0%
!! ACAAGTTTTGGTAGGAAATT+AGG - chr2.4:50429039-50429058 None:intergenic 30.0%
!! AGTTTTTCAGCGTTTTTGTT+CGG - chr2.4:50427142-50427161 None:intergenic 30.0%
!!! GTTTTGACTTTTCTGGAAAT+GGG + chr2.4:50427385-50427404 MS.gene025116:CDS 30.0%
AACAAGGTTCAACATGAGAT+TGG - chr2.4:50427331-50427350 None:intergenic 35.0%
ACGCCTTGGTAAAGATTATT+TGG + chr2.4:50429417-50429436 MS.gene025116:CDS 35.0%
ACTTTGAACTTGTGTTCGTT+TGG + chr2.4:50427683-50427702 MS.gene025116:intron 35.0%
AGCTAGGAACATGTATATTG+TGG - chr2.4:50428140-50428159 None:intergenic 35.0%
AGCTAGGGAGAGAAAAATTA+CGG - chr2.4:50428756-50428775 None:intergenic 35.0%
AGGATATGTTTCATGAAGTG+GGG - chr2.4:50428730-50428749 None:intergenic 35.0%
ATGGATTCTACAATCATGAC+AGG + chr2.4:50427006-50427025 MS.gene025116:CDS 35.0%
ATTCACAAAGATTGCAGGTT+AGG + chr2.4:50429815-50429834 MS.gene025116:intron 35.0%
CAACCAAAGTCAATCATTCT+CGG - chr2.4:50428421-50428440 None:intergenic 35.0%
GAAACCATTGACCACAATAA+GGG + chr2.4:50430318-50430337 MS.gene025116:CDS 35.0%
GAAAGACTTAATTGGATAGG+TGG - chr2.4:50428607-50428626 None:intergenic 35.0%
GAAGAGAGTTGAGTAATTTC+TGG + chr2.4:50428965-50428984 MS.gene025116:intron 35.0%
GACATTGTTATCTTGTGTCA+TGG + chr2.4:50427196-50427215 MS.gene025116:CDS 35.0%
GATCAAATTCATATCTGGCA+CGG - chr2.4:50429109-50429128 None:intergenic 35.0%
GCAAATTTGTCTAGACTGAT+TGG + chr2.4:50429265-50429284 MS.gene025116:intron 35.0%
GCAATTGTTGGACATAGTAA+AGG + chr2.4:50428000-50428019 MS.gene025116:CDS 35.0%
GGAGAGAAAAATTACGGTTA+AGG - chr2.4:50428750-50428769 None:intergenic 35.0%
GGATTACACGATTTGATTCA+CGG - chr2.4:50427978-50427997 None:intergenic 35.0%
GTAATCTTAACATCCAAAGC+AGG - chr2.4:50428925-50428944 None:intergenic 35.0%
GTTCCAGTATGGTAACTATT+GGG + chr2.4:50427915-50427934 MS.gene025116:CDS 35.0%
TAAACCCACAAGCTAAAGTT+AGG - chr2.4:50427872-50427891 None:intergenic 35.0%
TACTATGTCCAACAATTGCA+CGG - chr2.4:50427999-50428018 None:intergenic 35.0%
TCTAGACAAATTTGCGTGAA+AGG - chr2.4:50429260-50429279 None:intergenic 35.0%
TGCAATCTTTGTGAATCTGA+AGG - chr2.4:50429811-50429830 None:intergenic 35.0%
TTCAGATTCACAAAGATTGC+AGG + chr2.4:50429810-50429829 MS.gene025116:CDS 35.0%
TTCCAGTATGGTAACTATTG+GGG + chr2.4:50427916-50427935 MS.gene025116:CDS 35.0%
TTGGAAAGAATTACGAGTGA+CGG + chr2.4:50429436-50429455 MS.gene025116:CDS 35.0%
! ATCTCTTCTCCTTTTACTTC+TGG + chr2.4:50429852-50429871 MS.gene025116:intron 35.0%
! CTTAATTGGATAGGTGGATT+TGG - chr2.4:50428601-50428620 None:intergenic 35.0%
! GAGCATTTAGAGCAATTTAC+AGG - chr2.4:50428238-50428257 None:intergenic 35.0%
! TAAACTCAGGCTACAAGTTT+TGG - chr2.4:50429051-50429070 None:intergenic 35.0%
! TTTGGATTACCGTGTTACTA+AGG + chr2.4:50429742-50429761 MS.gene025116:CDS 35.0%
!! ATTTGTTGTTCAAGCATGTG+TGG + chr2.4:50429882-50429901 MS.gene025116:intron 35.0%
!! TTCTCCTAACTTTAGCTTGT+GGG + chr2.4:50427865-50427884 MS.gene025116:intron 35.0%
!! TTTCTCCTAACTTTAGCTTG+TGG + chr2.4:50427864-50427883 MS.gene025116:intron 35.0%
!!! CGTTTTGACTTTTCTGGAAA+TGG + chr2.4:50427384-50427403 MS.gene025116:CDS 35.0%
!!! CTCTCATTTGTTTTAGTAGC+TGG + chr2.4:50427091-50427110 MS.gene025116:intron 35.0%
!!! GTTTGCAGCATCTCTTTTTT+TGG - chr2.4:50428340-50428359 None:intergenic 35.0%
!!! TCTTTCCGTTTTGACTTTTC+TGG + chr2.4:50427378-50427397 MS.gene025116:CDS 35.0%
AAAACTGTTGTCAACCTCTC+TGG + chr2.4:50429364-50429383 MS.gene025116:CDS 40.0%
AAACTCGTGTGCACTTTGAA+CGG - chr2.4:50430193-50430212 None:intergenic 40.0%
ACTACAGGTAGGTATGGAAA+TGG - chr2.4:50430064-50430083 None:intergenic 40.0%
AGCTAACAGTACCCTTATTG+TGG - chr2.4:50430332-50430351 None:intergenic 40.0%
AGTACCCTTATTGTGGTCAA+TGG - chr2.4:50430325-50430344 None:intergenic 40.0%
AGTTCATCAACACTACAGGT+AGG - chr2.4:50430075-50430094 None:intergenic 40.0%
AGTTGTTATGAGCTTCATCG+AGG + chr2.4:50430296-50430315 MS.gene025116:CDS 40.0%
ATCAACACTACAGGTAGGTA+TGG - chr2.4:50430070-50430089 None:intergenic 40.0%
ATCTACTGTTGCAGGAAGTT+TGG + chr2.4:50429724-50429743 MS.gene025116:intron 40.0%
ATTGATGTGAAGACGAAGTC+AGG + chr2.4:50429463-50429482 MS.gene025116:CDS 40.0%
ATTTCATGTCAACCCTGCTT+TGG + chr2.4:50428909-50428928 MS.gene025116:intron 40.0%
CCATGCACCAAAATTATGAC+AGG + chr2.4:50428867-50428886 MS.gene025116:intron 40.0%
CGTTCCAGTATGGTAACTAT+TGG + chr2.4:50427914-50427933 MS.gene025116:CDS 40.0%
CTACAGGTAGGTATGGAAAT+GGG - chr2.4:50430063-50430082 None:intergenic 40.0%
GATATGTTTCATGAAGTGGG+GGG - chr2.4:50428728-50428747 None:intergenic 40.0%
GATGAAGCTCATAACAACTG+AGG - chr2.4:50430295-50430314 None:intergenic 40.0%
GGAAACCATTGACCACAATA+AGG + chr2.4:50430317-50430336 MS.gene025116:CDS 40.0%
GGAGAAGAGAGTTATTCCAA+TGG - chr2.4:50428682-50428701 None:intergenic 40.0%
GGATATGTTTCATGAAGTGG+GGG - chr2.4:50428729-50428748 None:intergenic 40.0%
GTCAATGTGAATGTCAACAC+AGG - chr2.4:50427739-50427758 None:intergenic 40.0%
GTCAGGTAAATTGTTTACCC+AGG + chr2.4:50429480-50429499 MS.gene025116:intron 40.0%
GTTGGCATATTGCATGAATC+TGG + chr2.4:50427163-50427182 MS.gene025116:CDS 40.0%
TCAGAACTCAGAAGTTACAC+TGG - chr2.4:50429210-50429229 None:intergenic 40.0%
TCCACACAAATAACGTGACA+GGG + chr2.4:50428311-50428330 MS.gene025116:intron 40.0%
TGTGGTCTCTATGAAAAGCT+AGG - chr2.4:50428156-50428175 None:intergenic 40.0%
TTCATATCTGGCACGGTTTA+AGG - chr2.4:50429102-50429121 None:intergenic 40.0%
TTGAGAACATCTACTGTTGC+AGG + chr2.4:50429716-50429735 MS.gene025116:intron 40.0%
TTGTGTGGAGTTCAATCTTG+CGG - chr2.4:50428300-50428319 None:intergenic 40.0%
! CGAGAATGATTGACTTTGGT+TGG + chr2.4:50428419-50428438 MS.gene025116:intron 40.0%
! CTGGTAGCAACACTCTTTTT+GGG - chr2.4:50428834-50428853 None:intergenic 40.0%
!! CAGATAGATTTTAACGGGCA+AGG + chr2.4:50429599-50429618 MS.gene025116:intron 40.0%
!! CCTGTCATAATTTTGGTGCA+TGG - chr2.4:50428870-50428889 None:intergenic 40.0%
!! TTTTGGTGCATGGAGAAAAG+CGG - chr2.4:50428860-50428879 None:intergenic 40.0%
AACTCGTGTGCACTTTGAAC+GGG - chr2.4:50430192-50430211 None:intergenic 45.0%
ACCCTGTCACGTTATTTGTG+TGG - chr2.4:50428315-50428334 None:intergenic 45.0%
CACAAAGATTGCAGGTTAGG+TGG + chr2.4:50429818-50429837 MS.gene025116:intron 45.0%
CAGAAGAACCGTGAACTGTA+AGG - chr2.4:50430158-50430177 None:intergenic 45.0%
CCAAAGTCAATCATTCTCGG+AGG - chr2.4:50428418-50428437 None:intergenic 45.0%
CCTTGTTGCTGCATTGGAAA+AGG + chr2.4:50427344-50427363 MS.gene025116:CDS 45.0%
CGTGTAATCCGTGCAATTGT+TGG + chr2.4:50427988-50428007 MS.gene025116:CDS 45.0%
CTCCACACAAATAACGTGAC+AGG + chr2.4:50428310-50428329 MS.gene025116:intron 45.0%
CTCCCCAATAGTTACCATAC+TGG - chr2.4:50427921-50427940 None:intergenic 45.0%
GGACGTTATGATCTGAAAGC+AGG + chr2.4:50429385-50429404 MS.gene025116:CDS 45.0%
GTATGGTAACTATTGGGGAG+AGG + chr2.4:50427921-50427940 MS.gene025116:CDS 45.0%
GTTGAACCTTGTTGCTGCAT+TGG + chr2.4:50427338-50427357 MS.gene025116:CDS 45.0%
TCAGATCATAACGTCCAGAG+AGG - chr2.4:50429381-50429400 None:intergenic 45.0%
TGAACCATGCCATCTATGTC+CGG + chr2.4:50427610-50427629 MS.gene025116:intron 45.0%
! ATAGGTGGATTTGGGTGGAT+GGG - chr2.4:50428592-50428611 None:intergenic 45.0%
! ATGTTTTCGTGTCGTGATGC+AGG + chr2.4:50429298-50429317 MS.gene025116:intron 45.0%
! ATTGGATAGGTGGATTTGGG+TGG - chr2.4:50428597-50428616 None:intergenic 45.0%
! CCTCCGAGAATGATTGACTT+TGG + chr2.4:50428415-50428434 MS.gene025116:intron 45.0%
! CCTTTTCCAATGCAGCAACA+AGG - chr2.4:50427347-50427366 None:intergenic 45.0%
! GCTGGTAGCAACACTCTTTT+TGG - chr2.4:50428835-50428854 None:intergenic 45.0%
! TCAACAGCTTTTGCAGCTGA+AGG - chr2.4:50428265-50428284 None:intergenic 45.0%
!! ACACTCAGTCGTTTTGCCAT+TGG + chr2.4:50428663-50428682 MS.gene025116:intron 45.0%
!! CAATTTGGTTTGCGAGAGTG+TGG - chr2.4:50428174-50428193 None:intergenic 45.0%
!! CTCAGGCTACAAGTTTTGGT+AGG - chr2.4:50429047-50429066 None:intergenic 45.0%
!! GTAGCAACACTCTTTTTGGG+AGG - chr2.4:50428831-50428850 None:intergenic 45.0%
!!! TTAATTTTTTTTTGTATATT+AGG + chr2.4:50427302-50427321 MS.gene025116:intron 5.0%
ACTCGTGTGCACTTTGAACG+GGG - chr2.4:50430191-50430210 None:intergenic 50.0%
AGCGAAGGTTCGTTCCAGTA+TGG + chr2.4:50427904-50427923 MS.gene025116:CDS 50.0%
AGCTTGTGGGTTTACCTTCC+AGG + chr2.4:50427878-50427897 MS.gene025116:intron 50.0%
AGTTCAATCTTGCGGTGGCT+CGG - chr2.4:50428292-50428311 None:intergenic 50.0%
ATCCTTCCAAACGAGCAACC+CGG - chr2.4:50427632-50427651 None:intergenic 50.0%
CCCTAGCTACTATGTACAGC+CGG + chr2.4:50428768-50428787 MS.gene025116:intron 50.0%
CCGGCTGTACATAGTAGCTA+GGG - chr2.4:50428771-50428790 None:intergenic 50.0%
GAACCATGCCATCTATGTCC+GGG + chr2.4:50427611-50427630 MS.gene025116:intron 50.0%
GCTTGTGGGTTTACCTTCCA+GGG + chr2.4:50427879-50427898 MS.gene025116:intron 50.0%
TGCTACACGGGGATCACATT+CGG - chr2.4:50428391-50428410 None:intergenic 50.0%
TGGTAACTATTGGGGAGAGG+TGG + chr2.4:50427924-50427943 MS.gene025116:CDS 50.0%
TGTGGAGTTCAATCTTGCGG+TGG - chr2.4:50428297-50428316 None:intergenic 50.0%
TGTTTCATGAAGTGGGGGGT+CGG - chr2.4:50428724-50428743 None:intergenic 50.0%
TTACCTTCCAGGGAAAGCGA+AGG + chr2.4:50427889-50427908 MS.gene025116:intron 50.0%
TTCCTCTTCCCCACTGAAGA+AGG + chr2.4:50428361-50428380 MS.gene025116:intron 50.0%
! AGCTTTTGCAGCTGAAGGTC+GGG - chr2.4:50428260-50428279 None:intergenic 50.0%
! CAGCTTTTGCAGCTGAAGGT+CGG - chr2.4:50428261-50428280 None:intergenic 50.0%
! GATAGGTGGATTTGGGTGGA+TGG - chr2.4:50428593-50428612 None:intergenic 50.0%
! TTCGGTGTGCCTTCTTCAGT+GGG - chr2.4:50428373-50428392 None:intergenic 50.0%
! TTTTCGTGTCGTGATGCAGG+AGG + chr2.4:50429301-50429320 MS.gene025116:intron 50.0%
!! ACTCTTTTTGGGAGGACCCA+TGG - chr2.4:50428823-50428842 None:intergenic 50.0%
!! ATTCGGTGTGCCTTCTTCAG+TGG - chr2.4:50428374-50428393 None:intergenic 50.0%
!! CACTGAAAGACGGTTTTGGC+CGG - chr2.4:50428790-50428809 None:intergenic 50.0%
!! CTCTTTTTGGGAGGACCCAT+GGG - chr2.4:50428822-50428841 None:intergenic 50.0%
!! GATGGTGGAGCACTGAAAGA+CGG - chr2.4:50428800-50428819 None:intergenic 50.0%
!!! GGAGCACTGAAAGACGGTTT+TGG - chr2.4:50428794-50428813 None:intergenic 50.0%
AACGGGCAAGGTTTCCATCG+CGG + chr2.4:50429611-50429630 MS.gene025116:intron 55.0%
ACGAGCAACCCGGACATAGA+TGG - chr2.4:50427622-50427641 None:intergenic 55.0%
CAACCCGGACATAGATGGCA+TGG - chr2.4:50427617-50427636 None:intergenic 55.0%
CTATGTCCGGGTTGCTCGTT+TGG + chr2.4:50427623-50427642 MS.gene025116:intron 55.0%
GAACCTTCGCTTTCCCTGGA+AGG - chr2.4:50427895-50427914 None:intergenic 55.0%
GAACGAACCTTCGCTTTCCC+TGG - chr2.4:50427899-50427918 None:intergenic 55.0%
GCCGGCTGTACATAGTAGCT+AGG - chr2.4:50428772-50428791 None:intergenic 55.0%
GGCAGGGTCCTTACAGTTCA+CGG + chr2.4:50430147-50430166 MS.gene025116:intron 55.0%
GTGGATTTGGGTGGATGGGA+GGG - chr2.4:50428588-50428607 None:intergenic 55.0%
TCAGTGCTCCACCATCCCAT+GGG + chr2.4:50428804-50428823 MS.gene025116:intron 55.0%
TCGGTGTGCCTTCTTCAGTG+GGG - chr2.4:50428372-50428391 None:intergenic 55.0%
TCTCGGAGGAACGTGCTACA+CGG - chr2.4:50428404-50428423 None:intergenic 55.0%
TGCCTTCTTCAGTGGGGAAG+AGG - chr2.4:50428366-50428385 None:intergenic 55.0%
TGGATTTGGGTGGATGGGAG+GGG - chr2.4:50428587-50428606 None:intergenic 55.0%
TTCAGTGCTCCACCATCCCA+TGG + chr2.4:50428803-50428822 MS.gene025116:intron 55.0%
!! TTTTGGGAGGACCCATGGGA+TGG - chr2.4:50428818-50428837 None:intergenic 55.0%
CTCGGAGGAACGTGCTACAC+GGG - chr2.4:50428403-50428422 None:intergenic 60.0%
GATTTGGGTGGATGGGAGGG+GGG - chr2.4:50428585-50428604 None:intergenic 60.0%
GCATGGAGAAAAGCGGCTGC+TGG - chr2.4:50428853-50428872 None:intergenic 60.0%
GGATTTGGGTGGATGGGAGG+GGG - chr2.4:50428586-50428605 None:intergenic 60.0%
GTCCGGGTTGCTCGTTTGGA+AGG + chr2.4:50427627-50427646 MS.gene025116:intron 60.0%
TCGGAGGAACGTGCTACACG+GGG - chr2.4:50428402-50428421 None:intergenic 60.0%
! GGAGAGTGTGGCTGTGGGAT+CGG - chr2.4:50428703-50428722 None:intergenic 60.0%
! GGTGGATTTGGGTGGATGGG+AGG - chr2.4:50428589-50428608 None:intergenic 60.0%
!! GCAGGCATCGAAGAACGCCT+TGG + chr2.4:50429403-50429422 MS.gene025116:CDS 60.0%
AAGTGGGGGGTCGGAGAGTG+TGG - chr2.4:50428715-50428734 None:intergenic 65.0%
TGGGAGGACCCATGGGATGG+TGG - chr2.4:50428815-50428834 None:intergenic 65.0%
! GGGTCGGAGAGTGTGGCTGT+GGG - chr2.4:50428708-50428727 None:intergenic 65.0%
! GGGGTCGGAGAGTGTGGCTG+TGG - chr2.4:50428709-50428728 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 50427006 50430353 50427006 ID=MS.gene025116
chr2.4 mRNA 50427006 50430353 50427006 ID=MS.gene025116.t1;Parent=MS.gene025116
chr2.4 exon 50427006 50427027 50427006 ID=MS.gene025116.t1.exon1;Parent=MS.gene025116.t1
chr2.4 CDS 50427006 50427027 50427006 ID=cds.MS.gene025116.t1;Parent=MS.gene025116.t1
chr2.4 exon 50427110 50427234 50427110 ID=MS.gene025116.t1.exon2;Parent=MS.gene025116.t1
chr2.4 CDS 50427110 50427234 50427110 ID=cds.MS.gene025116.t1;Parent=MS.gene025116.t1
chr2.4 exon 50427324 50427406 50427324 ID=MS.gene025116.t1.exon3;Parent=MS.gene025116.t1
chr2.4 CDS 50427324 50427406 50427324 ID=cds.MS.gene025116.t1;Parent=MS.gene025116.t1
chr2.4 exon 50427900 50428021 50427900 ID=MS.gene025116.t1.exon4;Parent=MS.gene025116.t1
chr2.4 CDS 50427900 50428021 50427900 ID=cds.MS.gene025116.t1;Parent=MS.gene025116.t1
chr2.4 exon 50429320 50429484 50429320 ID=MS.gene025116.t1.exon5;Parent=MS.gene025116.t1
chr2.4 CDS 50429320 50429484 50429320 ID=cds.MS.gene025116.t1;Parent=MS.gene025116.t1
chr2.4 exon 50429738 50429831 50429738 ID=MS.gene025116.t1.exon6;Parent=MS.gene025116.t1
chr2.4 CDS 50429738 50429831 50429738 ID=cds.MS.gene025116.t1;Parent=MS.gene025116.t1
chr2.4 exon 50430152 50430353 50430152 ID=MS.gene025116.t1.exon7;Parent=MS.gene025116.t1
chr2.4 CDS 50430152 50430353 50430152 ID=cds.MS.gene025116.t1;Parent=MS.gene025116.t1
Gene Sequence

>MS.gene025116

ATGGATTCTACAATCATGACAGGTATATTTACATTCTTGTTTGATATTACTTGTGATATATTGTATCTGTCTATGTTTACGTAATCTCTCATTTGTTTTAGTAGCTGGTCAGCGTCATCAGAGAGTTATAATACCGAACAAAAACGCTGAAAAACTTGTTGGCATATTGCATGAATCTGGAACAACGAACGACATTGTTATCTTGTGTCATGGTTTTCGTTGCACTAAAGTTTGTTACTTTTTCCTTTTCATTTATTATTCTATAGTTTGTTCCTTTGCCAGATTATTTTCATTGATTAATTTTTTTTTGTATATTAGGATACCAATCTCATGTTGAACCTTGTTGCTGCATTGGAAAAGGCACGAATTAGTTCTTTCCGTTTTGACTTTTCTGGAAATGGGTATGTTTATTTATGATGATGCATAACACAACCAGAGAGTTAATATTCAATTTTACATTGTTTGGATGTATAGAGATGGATAATGCAATATGTGTTATTTGATTCCATTTTATCAATTTTGATCTATTCATATGCTGTCAAAATATTTGTAAATTGAACTATACTACAAATTGATCGGCTAAAAGCTGAAAAATATAAAAAAGTGAACCATGCCATCTATGTCCGGGTTGCTCGTTTGGAAGGATACATTTATTCTTAGCTTGTATGTAGTTTATGACTTTGAACTTGTGTTCGTTTGGTGTGCATGATTAGTATGATTAAAGCATATACCTGTGTTGACATTCACATTGACTCGTAGACACTAGTATTTGAGAAAATAGAATTATTGAATTGGGTGGTATTTTTCTTCATGTACACTGTAGTTTCTGAAGTCTGATACCTTGTCTTTATTGTTTGCTTTCTCCTAACTTTAGCTTGTGGGTTTACCTTCCAGGGAAAGCGAAGGTTCGTTCCAGTATGGTAACTATTGGGGAGAGGTGGATGAATTACACACTGTTGTTCAACATTTCCGTGAATCAAATCGTGTAATCCGTGCAATTGTTGGACATAGTAAAGGTAAGTTGATTTGTTTGCTTTTACGAAAAAAGAATGTATATCTAGATATGTTTTTGGATATGTGATTATATGTGCCAATTTATATAATCATTCATTGATCATTTTCCATTATACACCACAATATACATGTTCCTAGCTTTTCATAGAGACCACACTCTCGCAAACCAAATTGAAGTAAAACACATTCTGAATACCTTTCTGCAATACAATAAACCTGTAAATTGCTCTAAATGCTCCCGACCTTCAGCTGCAAAAGCTGTTGAAACACCGAGCCACCGCAAGATTGAACTCCACACAAATAACGTGACAGGGTAGCCAAAAAAAGAGATGCTGCAAACTTTCCTCTTCCCCACTGAAGAAGGCACACCGAATGTGATCCCCGTGTAGCACGTTCCTCCGAGAATGATTGACTTTGGTTGGAAGTCTATCTTGAAGTAATCTCCAAACCATCACTGAAATTTTCAACGTAATCAAAGGGTTCCAAATGCACTGAACATAATTGTTATTAATGATAAGATAATTCTTTGTCAATTTATGGCTCGCTTTCTTAACAGAATACGCCCCCCTCCCATCCACCCAAATCCACCTATCCAATTAAGTCTTTCTGAAATCCACATTTTTTAAAAGACTAGAACATTCTAACACTCAGTCGTTTTGCCATTGGAATAACTCTCTTCTCCGATCCCACAGCCACACTCTCCGACCCCCCACTTCATGAAACATATCCTTAACCGTAATTTTTCTCTCCCTAGCTACTATGTACAGCCGGCCAAAACCGTCTTTCAGTGCTCCACCATCCCATGGGTCCTCCCAAAAAGAGTGTTGCTACCAGCAGCCGCTTTTCTCCATGCACCAAAATTATGACAGGAACTGTTCTTGTCAATATAATTTCATGTCAACCCTGCTTTGGATGTTAAGATTACAACCTTAAATAATGCAATATGAAGAGAGTTGAGTAATTTCTGGAAGAAAAAAAAGACCACAAATGAGCTTTCATTTAACTGATGTATAACACCTAATTTCCTACCAAAACTTGTAGCCTGAGTTTAGTAGTCAATAAGTTGAAATATAAAGAAACCTTAAACCGTGCCAGATATGAATTTGATCCTTATATTTCATTTTAAGATAGTTGTTACTTTTTAACTGGTACTATATTTTCATCTATAGTTCTTGAAAGTTGAATTGCCAGTGTAACTTCTGAGTTCTGACCAATCAATCTTCTCAAATTCTTATGTCCTTTCACGCAAATTTGTCTAGACTGATTGGACTAATAGTAATGTTTTCGTGTCGTGATGCAGGAGGAGACATAGTGCTTTTGTATGCTTCCAAATATCATGACATTAAAACTGTTGTCAACCTCTCTGGACGTTATGATCTGAAAGCAGGCATCGAAGAACGCCTTGGTAAAGATTATTTGGAAAGAATTACGAGTGACGGTTTTATTGATGTGAAGACGAAGTCAGGTAAATTGTTTACCCAGGCTTATAATATTACAAGAGCAAAGTTCGAGCTAGACATAATCGTGAACTATCTTGTTCAATAACTGCAAAATTACTTGAGTTTAAAACTTTAGAATTCAGATAGATTTTAACGGGCAAGGTTTCCATCGCGGATAATGAAAATTTACAAGTTCTTTGTTTCAATCTATGTAACACATATGCACTGAATTACTATGTTATTTATTAACTGATCATTTGAGAACATCTACTGTTGCAGGAAGTTTGGATTACCGTGTTACTAAGGAAAGTTTAATGGATCGCATGAATACAAACATGCATGAAGTGTGCCTTCAGATTCACAAAGATTGCAGGTTAGGTGGAAAGCTGTTTGATCTCTTCTCCTTTTACTTCTGGTCCATTTATTTGTTGTTCAAGCATGTGTGGCTGTTAAAATATCATGAATATTTCTACAATAGAAAAAATAAAAAACATGATCACTCTCAATTCTTATTATTATAGCAGTAAAATTAAATTTACTACATTTCCATACTTTTTTTAAGCTTAGCCATTAAGTGCAATTTTTGAAGAAAATTTAACTTCCCATTTCCATACCTACCTGTAGTGTTGATGAACTTATTAAGTTACATTCATGCATAGATATGCTGATGCCAAATTACATCATATATGGCAGGGTCCTTACAGTTCACGGTTCTTCTGACAAAATTATCCCCGTTCAAAGTGCACACGAGTTTGCTAAGATCATACCAAATCACAAGTTGCATATCATTAAACGAGCTAATCATGCATACAGTAATCATCAAGACGTGTTATCCTCAGTTGTTATGAGCTTCATCGAGGAAACCATTGACCACAATAAGGGTACTGTTAGCTAG
Protein sequence

>MS.gene025116.t1

MDSTIMTAGQRHQRVIIPNKNAEKLVGILHESGTTNDIVILCHGFRCTKDTNLMLNLVAALEKARISSFRFDFSGNGESEGSFQYGNYWGEVDELHTVVQHFRESNRVIRAIVGHSKGGDIVLLYASKYHDIKTVVNLSGRYDLKAGIEERLGKDYLERITSDGFIDVKTKSGSLDYRVTKESLMDRMNTNMHEVCLQIHKDCRVLTVHGSSDKIIPVQSAHEFAKIIPNHKLHIIKRANHAYSNHQDVLSSVVMSFIEETIDHNKGTVS