AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83168

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83168.t1 MTR_2g039510 79.926 269 52 2 1 267 1 269 1.80e-157 439
MS.gene83168.t1 MTR_2g039480 78.244 262 55 2 8 267 4 265 2.70e-149 418
MS.gene83168.t1 MTR_2g039450 73.134 268 70 2 1 267 1 267 7.37e-140 395
MS.gene83168.t1 MTR_2g039570 74.031 258 65 2 11 267 5 261 6.63e-138 389
MS.gene83168.t1 MTR_4g126050 68.914 267 80 3 1 267 57 320 2.09e-131 375
MS.gene83168.t1 MTR_4g126120 64.015 264 91 3 1 263 1 261 1.72e-122 350
MS.gene83168.t1 MTR_4g126120 64.427 253 87 2 12 263 20 270 1.72e-118 340
MS.gene83168.t1 MTR_1g016050 57.661 248 102 2 14 260 8 253 3.65e-96 284
MS.gene83168.t1 MTR_4g126050 70.492 183 51 3 1 183 57 236 2.82e-88 262
MS.gene83168.t1 MTR_1g016060 44.509 173 76 2 89 260 466 619 2.60e-33 128
MS.gene83168.t1 MTR_4g124530 60.526 38 15 0 132 169 29 66 4.49e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83168.t1 AT3G47590 62.400 250 91 2 14 263 59 305 2.72e-108 316
MS.gene83168.t1 AT1G29840 58.077 260 106 2 4 263 52 308 1.67e-104 306
MS.gene83168.t1 AT3G47560 61.290 248 93 2 13 260 9 253 9.39e-103 300
MS.gene83168.t1 AT3G47560 61.290 248 93 2 13 260 45 289 1.64e-102 301
MS.gene83168.t1 AT5G11910 60.000 250 97 2 12 260 38 285 2.76e-102 301
MS.gene83168.t1 AT5G11910 60.000 250 97 2 12 260 20 267 3.03e-102 300
MS.gene83168.t1 AT3G47560 55.678 273 94 2 13 260 9 279 1.01e-98 291
MS.gene83168.t1 AT3G47560 55.273 275 93 3 13 260 9 280 2.45e-96 285
MS.gene83168.t1 AT3G47560 50.667 300 94 3 13 260 9 306 2.49e-92 275
MS.gene83168.t1 AT2G19550 52.756 254 110 6 15 263 5 253 8.23e-86 259
MS.gene83168.t1 AT3G47590 65.605 157 52 1 14 170 59 213 1.37e-68 212
MS.gene83168.t1 AT5G11910 60.839 143 54 1 12 154 38 178 8.85e-57 180
MS.gene83168.t1 AT5G11910 60.839 143 54 1 12 154 20 160 1.48e-56 179

Find 39 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATCTTGGTAAAGATTATT+TGG 0.156761 2.4:+50491235 MS.gene83168:CDS
AAGATTATTTGGAAAGAATT+AGG 0.246216 2.4:+50491246 MS.gene83168:CDS
GGATTACACGATTTGCTTCA+TGG 0.317005 2.4:-50490830 None:intergenic
GGCGTATTACATGAAACTTC+TGG 0.335418 2.4:+50489616 MS.gene83168:CDS
AGTAACCTTGTTGAGGTTCA+TGG 0.338262 2.4:-50491769 None:intergenic
CGTGTAATCCGTGCAATTGT+TGG 0.348094 2.4:+50490843 MS.gene83168:CDS
CGTTTCAGAACGGTAACTAT+TGG 0.372214 2.4:+50490769 MS.gene83168:CDS
CACAAGTTGCATGTCATTGA+AGG 0.457279 2.4:+50491678 MS.gene83168:CDS
AAAACTGTTGTCAACCTCTC+TGG 0.459812 2.4:+50491182 MS.gene83168:CDS
GGCAGGGTCCTTACAGTTCA+CGG 0.470147 2.4:+50491594 MS.gene83168:intron
AGCAAGGTTCACTATGAGAT+TGG 0.470630 2.4:-50489787 None:intergenic
TTCATTGATGTGAAGAGGCC+AGG 0.473908 2.4:+50491278 MS.gene83168:CDS
TCAAAATTTCTTCAGTAAGA+CGG 0.490951 2.4:-50491425 None:intergenic
GACATTGTTATCTTGTGTCA+TGG 0.497964 2.4:+50489649 MS.gene83168:CDS
CTTCAATGACATGCAACTTG+TGG 0.505270 2.4:-50491677 None:intergenic
TACTGTGTCCAACAATTGCA+CGG 0.513231 2.4:-50490851 None:intergenic
TGCATTCTCTGTCAATATGA+AGG 0.516067 2.4:-50491482 None:intergenic
TTGGAAAGAATTAGGAAGGA+CGG 0.522675 2.4:+50491254 MS.gene83168:CDS
CGGAAGAACCGTGAACTGTA+AGG 0.528085 2.4:-50491602 None:intergenic
TTATTTGGAAAGAATTAGGA+AGG 0.529949 2.4:+50491250 MS.gene83168:CDS
AGTTGTTGTCAGCTTCATCA+AGG 0.532584 2.4:+50491743 MS.gene83168:CDS
CCTTGCTGATGCATTGGAAA+AGG 0.541626 2.4:+50489803 MS.gene83168:CDS
AGTGAACCTTGCTGATGCAT+TGG 0.541684 2.4:+50489797 MS.gene83168:CDS
GCAATTGTTGGACACAGTAA+AGG 0.550180 2.4:+50490855 MS.gene83168:CDS
CTCTGGACGTTATGATCTGA+AGG 0.550517 2.4:+50491199 MS.gene83168:CDS
GCAGGCATCGAAGAACATCT+TGG 0.554713 2.4:+50491221 MS.gene83168:CDS
TTGAACGGAGTTAATTTCAT+CGG 0.556866 2.4:-50491622 None:intergenic
CATTTCCTGAAAAGTCAAAA+CGG 0.561669 2.4:-50489842 None:intergenic
ACGGTTTCATTGATGTGAAG+AGG 0.562563 2.4:+50491273 MS.gene83168:CDS
GGACGTTATGATCTGAAGGC+AGG 0.563099 2.4:+50491203 MS.gene83168:CDS
TTCATATTGACAGAGAATGC+AGG 0.592777 2.4:+50491484 MS.gene83168:CDS
AGCTAGCAGTAACCTTGTTG+AGG 0.594917 2.4:-50491776 None:intergenic
CGGTAACTATTGGACAGAGG+TGG 0.646830 2.4:+50490779 MS.gene83168:CDS
AGTGAAGGTTCGTTTCAGAA+CGG 0.652849 2.4:+50490759 MS.gene83168:CDS
GGAAACCATGAACCTCAACA+AGG 0.682330 2.4:+50491764 MS.gene83168:CDS
AAACGCGTATGCATCTTGAA+CGG 0.689910 2.4:-50491637 None:intergenic
GATGAAGCTGACAACAACTG+AGG 0.711495 2.4:-50491739 None:intergenic
TCAGATCATAACGTCCAGAG+AGG 0.713623 2.4:-50491196 None:intergenic
GAACGGTAACTATTGGACAG+AGG 0.732928 2.4:+50490776 MS.gene83168:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAATTATATATAATGT+AGG - chr2.4:50489486-50489505 None:intergenic 10.0%
!! CATATTAAGTGAATATATAT+AGG + chr2.4:50490021-50490040 MS.gene83168:intron 15.0%
!! CTATATATATTCACTTAATA+TGG - chr2.4:50490023-50490042 None:intergenic 15.0%
!! GTTTGATTATATGAAAAATA+AGG + chr2.4:50490889-50490908 MS.gene83168:intron 15.0%
!! TTCAATCGAATTTAAATTAT+TGG + chr2.4:50490090-50490109 MS.gene83168:intron 15.0%
!! AACATGATATTATCTTTATC+AGG + chr2.4:50490452-50490471 MS.gene83168:intron 20.0%
!! AAGATTATTTGGAAAGAATT+AGG + chr2.4:50491246-50491265 MS.gene83168:CDS 20.0%
!! ACTCTTAATTATTTGATCTT+CGG - chr2.4:50490294-50490313 None:intergenic 20.0%
!! ATGTCAAATTACATCATATA+TGG + chr2.4:50491573-50491592 MS.gene83168:intron 20.0%
!! ATTAAAGTTGAAACAAAGTA+AGG - chr2.4:50490717-50490736 None:intergenic 20.0%
!! TGCATACATTTAACTAAAAA+AGG - chr2.4:50489705-50489724 None:intergenic 20.0%
!! TTGTTTCAACTTTAATGAAT+GGG + chr2.4:50490721-50490740 MS.gene83168:intron 20.0%
!! TTTGTTTCAACTTTAATGAA+TGG + chr2.4:50490720-50490739 MS.gene83168:intron 20.0%
!!! AGTTTTAATGTCATGATATT+TGG - chr2.4:50491168-50491187 None:intergenic 20.0%
! AAATTACATCATATATGGCA+GGG + chr2.4:50491578-50491597 MS.gene83168:intron 25.0%
! ACATCTTGGTAAAGATTATT+TGG + chr2.4:50491235-50491254 MS.gene83168:CDS 25.0%
! ATAATTGATCTAGATGATCA+AGG + chr2.4:50489894-50489913 MS.gene83168:intron 25.0%
! ATATCTATGCATCAATGTTA+AGG - chr2.4:50491549-50491568 None:intergenic 25.0%
! ATCATAATACCGAACAAAAA+CGG + chr2.4:50489580-50489599 MS.gene83168:CDS 25.0%
! ATGTTGTATTGAAAACTGAA+AGG + chr2.4:50489931-50489950 MS.gene83168:intron 25.0%
! CAACGAATGATTGTATAAAT+CGG - chr2.4:50490953-50490972 None:intergenic 25.0%
! CCTTAATCTTTCTTCTTTAA+AGG - chr2.4:50490376-50490395 None:intergenic 25.0%
! TATAATGTAGGAAACTAACA+TGG - chr2.4:50489474-50489493 None:intergenic 25.0%
! TATCTATGCATCAATGTTAA+GGG - chr2.4:50491548-50491567 None:intergenic 25.0%
! TCAAAATTTCTTCAGTAAGA+CGG - chr2.4:50491428-50491447 None:intergenic 25.0%
! TGTTGTATTGAAAACTGAAA+GGG + chr2.4:50489932-50489951 MS.gene83168:intron 25.0%
! TTATTTGGAAAGAATTAGGA+AGG + chr2.4:50491250-50491269 MS.gene83168:CDS 25.0%
!! CCTTTAAAGAAGAAAGATTA+AGG + chr2.4:50490373-50490392 MS.gene83168:intron 25.0%
!! GACAATAGCTTAGATATTTT+GGG + chr2.4:50489970-50489989 MS.gene83168:intron 25.0%
!! TCTTACTGAAGAAATTTTGA+AGG + chr2.4:50491428-50491447 MS.gene83168:CDS 25.0%
!! TTAAATTCGATTGAAAGTGT+TGG - chr2.4:50490085-50490104 None:intergenic 25.0%
!!! AAACTTTAGAACGTATTACT+CGG - chr2.4:50490670-50490689 None:intergenic 25.0%
!!! TGTTTTGTTTTTTCCAATGT+AGG + chr2.4:50489761-50489780 MS.gene83168:intron 25.0%
AAAAACGGTGAAAAACTTGT+TGG + chr2.4:50489595-50489614 MS.gene83168:CDS 30.0%
AAGAAACAAACGACAAAATC+AGG - chr2.4:50490569-50490588 None:intergenic 30.0%
ACACAAGAAATTTGAGAAGA+AGG - chr2.4:50491051-50491070 None:intergenic 30.0%
AGAAATTTGAGAAGAAGGAT+TGG - chr2.4:50491046-50491065 None:intergenic 30.0%
ATGAACATGGGTATTCTAAT+TGG + chr2.4:50490999-50491018 MS.gene83168:intron 30.0%
CAAATTACATCATATATGGC+AGG + chr2.4:50491577-50491596 MS.gene83168:intron 30.0%
CATTTCCTGAAAAGTCAAAA+CGG - chr2.4:50489845-50489864 None:intergenic 30.0%
CGAAAGATTTATCTCTCATT+TGG + chr2.4:50489526-50489545 MS.gene83168:intron 30.0%
GCATGTTCAATGATTAAATC+AGG - chr2.4:50490482-50490501 None:intergenic 30.0%
GGCAAAGAACAATTCAATAA+CGG - chr2.4:50490519-50490538 None:intergenic 30.0%
GGGTAAGTTTATTGTTACAT+TGG + chr2.4:50489864-50489883 MS.gene83168:intron 30.0%
GTTGTATTGAAAACTGAAAG+GGG + chr2.4:50489933-50489952 MS.gene83168:intron 30.0%
TATAAGGAAATCAAGTCACT+GGG - chr2.4:50490149-50490168 None:intergenic 30.0%
TCAATCATTATTGCAAACCT+TGG - chr2.4:50491085-50491104 None:intergenic 30.0%
TTGAACGGAGTTAATTTCAT+CGG - chr2.4:50491625-50491644 None:intergenic 30.0%
! GGACAATAGCTTAGATATTT+TGG + chr2.4:50489969-50489988 MS.gene83168:intron 30.0%
! GGTATATGAAGTTATGCTTT+TGG + chr2.4:50490910-50490929 MS.gene83168:intron 30.0%
!! AATGAATGGGTTTTCTTCAA+GGG + chr2.4:50490734-50490753 MS.gene83168:intron 30.0%
!! AGTTTTTCACCGTTTTTGTT+CGG - chr2.4:50489592-50489611 None:intergenic 30.0%
!! ATAGCTTAGATATTTTGGGA+CGG + chr2.4:50489974-50489993 MS.gene83168:intron 30.0%
!! GAAATTTTGAAGGATTGCTT+AGG + chr2.4:50491438-50491457 MS.gene83168:CDS 30.0%
!! TAATGAATGGGTTTTCTTCA+AGG + chr2.4:50490733-50490752 MS.gene83168:intron 30.0%
!!! GTTTTGACTTTTCAGGAAAT+GGG + chr2.4:50489844-50489863 MS.gene83168:CDS 30.0%
AAATTTCAACGTGAACGATG+CGG + chr2.4:50491302-50491321 MS.gene83168:intron 35.0%
AACACGGCGATAAAACTATA+AGG - chr2.4:50490165-50490184 None:intergenic 35.0%
ACCTAATTTACACAAGCGAA+AGG - chr2.4:50490629-50490648 None:intergenic 35.0%
ATAAGGAAATCAAGTCACTG+GGG - chr2.4:50490148-50490167 None:intergenic 35.0%
ATGAGATTGGTATCCTACAT+TGG - chr2.4:50489777-50489796 None:intergenic 35.0%
CAACTATATGACTCATATCC+AGG - chr2.4:50490352-50490371 None:intergenic 35.0%
CCTTACTAGACTTAGATATG+CGG + chr2.4:50490199-50490218 MS.gene83168:intron 35.0%
CTATAAGGAAATCAAGTCAC+TGG - chr2.4:50490150-50490169 None:intergenic 35.0%
CTGGATATGAGTCATATAGT+TGG + chr2.4:50490350-50490369 MS.gene83168:intron 35.0%
GAAGAAAGATTAAGGTTCCT+AGG + chr2.4:50490381-50490400 MS.gene83168:intron 35.0%
GACATTGTTATCTTGTGTCA+TGG + chr2.4:50489649-50489668 MS.gene83168:CDS 35.0%
GCTATTGTCCCAAAATGATT+TGG - chr2.4:50489959-50489978 None:intergenic 35.0%
TAGAATACCCATGTTCATAC+CGG - chr2.4:50490997-50491016 None:intergenic 35.0%
TCATTCGTTGAAGAAAAACC+AGG + chr2.4:50490963-50490982 MS.gene83168:intron 35.0%
TCCTTTCGCTTGTGTAAATT+AGG + chr2.4:50490625-50490644 MS.gene83168:intron 35.0%
TGCATTCTCTGTCAATATGA+AGG - chr2.4:50491485-50491504 None:intergenic 35.0%
TTCATATTGACAGAGAATGC+AGG + chr2.4:50491484-50491503 MS.gene83168:CDS 35.0%
TTGGAAAGAATTAGGAAGGA+CGG + chr2.4:50491254-50491273 MS.gene83168:CDS 35.0%
TTTCGCTTGTGTAAATTAGG+TGG + chr2.4:50490628-50490647 MS.gene83168:intron 35.0%
! GAACAATTCAATAACGGAAC+TGG - chr2.4:50490513-50490532 None:intergenic 35.0%
! TTTTCTTCAAGGGAAAGTGA+AGG + chr2.4:50490744-50490763 MS.gene83168:intron 35.0%
!!! CGTTTTGACTTTTCAGGAAA+TGG + chr2.4:50489843-50489862 MS.gene83168:CDS 35.0%
!!! TAGCTTAGATATTTTGGGAC+GGG + chr2.4:50489975-50489994 MS.gene83168:intron 35.0%
!!! TCTTTCCGTTTTGACTTTTC+AGG + chr2.4:50489837-50489856 MS.gene83168:CDS 35.0%
!!! TTGTTTTTGTGTCCTGATGT+AGG + chr2.4:50491116-50491135 MS.gene83168:intron 35.0%
AAAACTGTTGTCAACCTCTC+TGG + chr2.4:50491182-50491201 MS.gene83168:CDS 40.0%
AAACGCGTATGCATCTTGAA+CGG - chr2.4:50491640-50491659 None:intergenic 40.0%
AATTAAGAGTCGTGAGTGTG+AGG + chr2.4:50490304-50490323 MS.gene83168:intron 40.0%
ACGGTTTCATTGATGTGAAG+AGG + chr2.4:50491273-50491292 MS.gene83168:CDS 40.0%
AGCAAGGTTCACTATGAGAT+TGG - chr2.4:50489790-50489809 None:intergenic 40.0%
AGTGAAGGTTCGTTTCAGAA+CGG + chr2.4:50490759-50490778 MS.gene83168:CDS 40.0%
AGTTGTTGTCAGCTTCATCA+AGG + chr2.4:50491743-50491762 MS.gene83168:CDS 40.0%
CACAAGTTGCATGTCATTGA+AGG + chr2.4:50491678-50491697 MS.gene83168:CDS 40.0%
CCAAAATAACTGCCCAGTTA+TGG - chr2.4:50490415-50490434 None:intergenic 40.0%
CCGCATATCTAAGTCTAGTA+AGG - chr2.4:50490202-50490221 None:intergenic 40.0%
CGTTCACGTTGAAATTTACC+TGG - chr2.4:50491299-50491318 None:intergenic 40.0%
CGTTTCAGAACGGTAACTAT+TGG + chr2.4:50490769-50490788 MS.gene83168:CDS 40.0%
CTGCGAACATATATTGTTGC+AGG + chr2.4:50491390-50491409 MS.gene83168:intron 40.0%
CTTCAATGACATGCAACTTG+TGG - chr2.4:50491680-50491699 None:intergenic 40.0%
GAATAGAAGTTGCTCCATAG+AGG + chr2.4:50490246-50490265 MS.gene83168:intron 40.0%
GCAATTGTTGGACACAGTAA+AGG + chr2.4:50490855-50490874 MS.gene83168:CDS 40.0%
GGATCAGTTAGCATCTAACA+CGG - chr2.4:50490181-50490200 None:intergenic 40.0%
GGCGTATTACATGAAACTTC+TGG + chr2.4:50489616-50489635 MS.gene83168:CDS 40.0%
TACTGTGTCCAACAATTGCA+CGG - chr2.4:50490854-50490873 None:intergenic 40.0%
TAGGACCCAAAACCATAACT+GGG + chr2.4:50490400-50490419 MS.gene83168:intron 40.0%
TAGGGTTAGAATCATGTACC+TGG + chr2.4:50490331-50490350 MS.gene83168:intron 40.0%
TGCAAACCTTGGCAGAATTA+AGG - chr2.4:50491074-50491093 None:intergenic 40.0%
TTGTGTCCTTAATTCTGCCA+AGG + chr2.4:50491065-50491084 MS.gene83168:intron 40.0%
! AACATGGATTTTGTGCAGCT+AGG - chr2.4:50489458-50489477 None:intergenic 40.0%
! AACTGCCCAGTTATGGTTTT+GGG - chr2.4:50490408-50490427 None:intergenic 40.0%
! ACATGGATTTTGTGCAGCTA+GGG - chr2.4:50489457-50489476 None:intergenic 40.0%
! CCATAACTGGGCAGTTATTT+TGG + chr2.4:50490412-50490431 MS.gene83168:intron 40.0%
! GAAAGGGGCCAAATCATTTT+GGG + chr2.4:50489948-50489967 MS.gene83168:intron 40.0%
! GGATTACACGATTTGCTTCA+TGG - chr2.4:50490833-50490852 None:intergenic 40.0%
! TAACTGCCCAGTTATGGTTT+TGG - chr2.4:50490409-50490428 None:intergenic 40.0%
! TCGTGAGTGTGAGGTTTTTA+GGG + chr2.4:50490313-50490332 MS.gene83168:intron 40.0%
! TGAAAGGGGCCAAATCATTT+TGG + chr2.4:50489947-50489966 MS.gene83168:intron 40.0%
! TTTTTGTGTCCTGATGTAGG+AGG + chr2.4:50491119-50491138 MS.gene83168:intron 40.0%
!! TGAAAGTGTTGGTGCTACAT+AGG - chr2.4:50490074-50490093 None:intergenic 40.0%
!!! AACTGGGCAGTTATTTTGGA+TGG + chr2.4:50490416-50490435 MS.gene83168:intron 40.0%
AATTGTTCTTTGCCCAGTCC+GGG + chr2.4:50490525-50490544 MS.gene83168:intron 45.0%
ACTTCGATACCTCCTACATC+AGG - chr2.4:50491131-50491150 None:intergenic 45.0%
AGTGAACCTTGCTGATGCAT+TGG + chr2.4:50489797-50489816 MS.gene83168:CDS 45.0%
CCTTGCTGATGCATTGGAAA+AGG + chr2.4:50489803-50489822 MS.gene83168:CDS 45.0%
CGTGTAATCCGTGCAATTGT+TGG + chr2.4:50490843-50490862 MS.gene83168:CDS 45.0%
CTAGGACCCAAAACCATAAC+TGG + chr2.4:50490399-50490418 MS.gene83168:intron 45.0%
CTCTGGACGTTATGATCTGA+AGG + chr2.4:50491199-50491218 MS.gene83168:CDS 45.0%
GAAAAACCAGGAATGTGCAC+CGG + chr2.4:50490975-50490994 MS.gene83168:intron 45.0%
GAACGGTAACTATTGGACAG+AGG + chr2.4:50490776-50490795 MS.gene83168:CDS 45.0%
GAATTGTTCTTTGCCCAGTC+CGG + chr2.4:50490524-50490543 MS.gene83168:intron 45.0%
GATGAAGCTGACAACAACTG+AGG - chr2.4:50491742-50491761 None:intergenic 45.0%
TAGAAGTTGCTCCATAGAGG+TGG + chr2.4:50490249-50490268 MS.gene83168:intron 45.0%
TAGACTTAGATATGCGGCCT+GGG + chr2.4:50490205-50490224 MS.gene83168:intron 45.0%
TCAGATCATAACGTCCAGAG+AGG - chr2.4:50491199-50491218 None:intergenic 45.0%
TTCATTGATGTGAAGAGGCC+AGG + chr2.4:50491278-50491297 MS.gene83168:CDS 45.0%
! AATGTGCACCGGTATGAACA+TGG + chr2.4:50490986-50491005 MS.gene83168:intron 45.0%
! ATGTGCACCGGTATGAACAT+GGG + chr2.4:50490987-50491006 MS.gene83168:intron 45.0%
! CCTTTTCCAATGCATCAGCA+AGG - chr2.4:50489806-50489825 None:intergenic 45.0%
! GTCGTGAGTGTGAGGTTTTT+AGG + chr2.4:50490312-50490331 MS.gene83168:intron 45.0%
!!! CAGTTATGGTTTTGGGTCCT+AGG - chr2.4:50490401-50490420 None:intergenic 45.0%
!!! CTTAGATATTTTGGGACGGG+TGG + chr2.4:50489978-50489997 MS.gene83168:intron 45.0%
!!! GTTATTTTGGATGGCAGCTG+TGG + chr2.4:50490425-50490444 MS.gene83168:intron 45.0%
!! TTTCAAAAAAATAAATAAAA+TGG + chr2.4:50489502-50489521 MS.gene83168:intron 5.0%
AAGTTGCTCCATAGAGGTGG+TGG + chr2.4:50490252-50490271 MS.gene83168:intron 50.0%
AGACTTAGATATGCGGCCTG+GGG + chr2.4:50490206-50490225 MS.gene83168:intron 50.0%
ATTGTTCTTTGCCCAGTCCG+GGG + chr2.4:50490526-50490545 MS.gene83168:intron 50.0%
CAAGTACACCACCACCTCTA+TGG - chr2.4:50490263-50490282 None:intergenic 50.0%
CGGAAGAACCGTGAACTGTA+AGG - chr2.4:50491605-50491624 None:intergenic 50.0%
CGGTAACTATTGGACAGAGG+TGG + chr2.4:50490779-50490798 MS.gene83168:CDS 50.0%
CTAGACTTAGATATGCGGCC+TGG + chr2.4:50490204-50490223 MS.gene83168:intron 50.0%
GGACGTTATGATCTGAAGGC+AGG + chr2.4:50491203-50491222 MS.gene83168:CDS 50.0%
TCACTCATAAGTCACTGCCC+CGG - chr2.4:50490546-50490565 None:intergenic 50.0%
TGACAGAGAATGCAGGTTCG+CGG + chr2.4:50491491-50491510 MS.gene83168:intron 50.0%
TTCATACCGGTGCACATTCC+TGG - chr2.4:50490984-50491003 None:intergenic 50.0%
!! GCAGGCATCGAAGAACATCT+TGG + chr2.4:50491221-50491240 MS.gene83168:CDS 50.0%
ATAAGTCACTGCCCCGGACT+GGG - chr2.4:50490540-50490559 None:intergenic 55.0%
GACTTAGATATGCGGCCTGG+GGG + chr2.4:50490207-50490226 MS.gene83168:intron 55.0%
GGCAGGGTCCTTACAGTTCA+CGG + chr2.4:50491594-50491613 MS.gene83168:intron 55.0%
CATAAGTCACTGCCCCGGAC+TGG - chr2.4:50490541-50490560 None:intergenic 60.0%
CAGCCACTCAACTGTCCCCC+AGG - chr2.4:50490225-50490244 None:intergenic 65.0%
CGGCCTGGGGGACAGTTGAG+TGG + chr2.4:50490219-50490238 MS.gene83168:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 50489450 50491785 50489450 ID=MS.gene83168
chr2.4 mRNA 50489450 50491785 50489450 ID=MS.gene83168.t1;Parent=MS.gene83168
chr2.4 exon 50489450 50489474 50489450 ID=MS.gene83168.t1.exon1;Parent=MS.gene83168.t1
chr2.4 CDS 50489450 50489474 50489450 ID=cds.MS.gene83168.t1;Parent=MS.gene83168.t1
chr2.4 exon 50489560 50489687 50489560 ID=MS.gene83168.t1.exon2;Parent=MS.gene83168.t1
chr2.4 CDS 50489560 50489687 50489560 ID=cds.MS.gene83168.t1;Parent=MS.gene83168.t1
chr2.4 exon 50489783 50489865 50489783 ID=MS.gene83168.t1.exon3;Parent=MS.gene83168.t1
chr2.4 CDS 50489783 50489865 50489783 ID=cds.MS.gene83168.t1;Parent=MS.gene83168.t1
chr2.4 exon 50490755 50490876 50490755 ID=MS.gene83168.t1.exon4;Parent=MS.gene83168.t1
chr2.4 CDS 50490755 50490876 50490755 ID=cds.MS.gene83168.t1;Parent=MS.gene83168.t1
chr2.4 exon 50491138 50491299 50491138 ID=MS.gene83168.t1.exon5;Parent=MS.gene83168.t1
chr2.4 CDS 50491138 50491299 50491138 ID=cds.MS.gene83168.t1;Parent=MS.gene83168.t1
chr2.4 exon 50491412 50491505 50491412 ID=MS.gene83168.t1.exon6;Parent=MS.gene83168.t1
chr2.4 CDS 50491412 50491505 50491412 ID=cds.MS.gene83168.t1;Parent=MS.gene83168.t1
chr2.4 exon 50491599 50491785 50491599 ID=MS.gene83168.t1.exon7;Parent=MS.gene83168.t1
chr2.4 CDS 50491599 50491785 50491599 ID=cds.MS.gene83168.t1;Parent=MS.gene83168.t1
Gene Sequence

>MS.gene83168

ATGTCCCTAGCTGCACAAAATCCATGTTAGTTTCCTACATTATATATAATTTTTTCAAAAAAATAAATAAAATGGACGAAAGATTTATCTCTCATTTGGTGTTTTTCTAGCTGTTGAGCATCATCAAAGAATCATAATACCGAACAAAAACGGTGAAAAACTTGTTGGCGTATTACATGAAACTTCTGGAACAAGCAACGACATTGTTATCTTGTGTCATGGCTTTCGTTGCTCTAAAGTTAGTTACTTTTTCCTTTTTTAGTTAAATGTATGCATACATTCTTTTACTAGATTATTTTAATTAATTTGTATGTTTTGTTTTTTCCAATGTAGGATACCAATCTCATAGTGAACCTTGCTGATGCATTGGAAAAGGCACAAATTAGTTCTTTCCGTTTTGACTTTTCAGGAAATGGGTAAGTTTATTGTTACATTGGTCGCAAAATAATTGATCTAGATGATCAAGGCTCAAAAGTAATTAATGTTGTATTGAAAACTGAAAGGGGCCAAATCATTTTGGGACAATAGCTTAGATATTTTGGGACGGGTGGAATGTAACATTGAACACAACCATATTAAGTGAATATATATAGGATTGCTGTGATTTCATGTAGTTTATGACCTATGTAGCACCAACACTTTCAATCGAATTTAAATTATTGGTTTATTTGTAATACTAAAGACTATGATACTGTCCCCAGTGACTTGATTTCCTTATAGTTTTATCGCCGTGTTAGATGCTAACTGATCCTTACTAGACTTAGATATGCGGCCTGGGGGACAGTTGAGTGGCTGAGAATAGAAGTTGCTCCATAGAGGTGGTGGTGTACTTGCAAACACTCCGAAGATCAAATAATTAAGAGTCGTGAGTGTGAGGTTTTTAGGGTTAGAATCATGTACCTGGATATGAGTCATATAGTTGGCCTTTAAAGAAGAAAGATTAAGGTTCCTAGGACCCAAAACCATAACTGGGCAGTTATTTTGGATGGCAGCTGTGGACAGAACATGATATTATCTTTATCAGGCTGTCCTGATTTAATCATTGAACATGCTCGAGTGACCAGTTCCGTTATTGAATTGTTCTTTGCCCAGTCCGGGGCAGTGACTTATGAGTGACCTGATTTTGTCGTTTGTTTCTTCTGTGATTCAACAATATACAAATAATATTAATGATCTCCTTTCGCTTGTGTAAATTAGGTGGTATTTCTTCATTTACACCGAGTAATACGTTCTAAAGTTTATGATATATTGTCTTCATCATTAGCCTTACTTTGTTTCAACTTTAATGAATGGGTTTTCTTCAAGGGAAAGTGAAGGTTCGTTTCAGAACGGTAACTATTGGACAGAGGTGGATGATTTACAATCTGTTGCTCAACATTTCCATGAAGCAAATCGTGTAATCCGTGCAATTGTTGGACACAGTAAAGGTATCTTGATTTGTTTGATTATATGAAAAATAAGGTATATGAAGTTATGCTTTTGGATATACGATTATATGTGCCGATTTATACAATCATTCGTTGAAGAAAAACCAGGAATGTGCACCGGTATGAACATGGGTATTCTAATTGGTTGTCAGTGCATTTCTAATGACCAATCCTTCTTCTCAAATTTCTTGTGTCCTTAATTCTGCCAAGGTTTGCAATAATGATTGAACTATTAGCAATTGTTTTTGTGTCCTGATGTAGGAGGTATCGAAGTTCTTCTCTATGCATCCAAATATCATGACATTAAAACTGTTGTCAACCTCTCTGGACGTTATGATCTGAAGGCAGGCATCGAAGAACATCTTGGTAAAGATTATTTGGAAAGAATTAGGAAGGACGGTTTCATTGATGTGAAGAGGCCAGGTAAATTTCAACGTGAACGATGCGGATAATGAAAATTTATAAGTTATATTTTCAATCTTTCAATTACTATATAATTTATTAAATGATCGTCTGCGAACATATATTGTTGCAGGAAGTTCTGATTACCGTCTTACTGAAGAAATTTTGAAGGATTGCTTAGGTATAAATATACATGAAGCATGCCTTCATATTGACAGAGAATGCAGGTTCGCGGAAAAGATGCTTGATCTCTTCTGCTTCATAGTCCCTTAACATTGATGCATAGATATGCTAATGTCAAATTACATCATATATGGCAGGGTCCTTACAGTTCACGGTTCTTCCGATGAAATTAACTCCGTTCAAGATGCATACGCGTTTGATAAGATCATATCAAACCACAAGTTGCATGTCATTGAAGGAGCTGATCATGCATACAATAATCATCAAAATGAGTTATCCTCAGTTGTTGTCAGCTTCATCAAGGAAACCATGAACCTCAACAAG
Protein sequence

>MS.gene83168.t1

MSLAAQNPSVEHHQRIIIPNKNGEKLVGVLHETSGTSNDIVILCHGFRCSKDTNLIVNLADALEKAQISSFRFDFSGNGESEGSFQNGNYWTEVDDLQSVAQHFHEANRVIRAIVGHSKGGIEVLLYASKYHDIKTVVNLSGRYDLKAGIEEHLGKDYLERIRKDGFIDVKRPGSSDYRLTEEILKDCLGINIHEACLHIDRECRVLTVHGSSDEINSVQDAYAFDKIISNHKLHVIEGADHAYNNHQNELSSVVVSFIKETMNLNK