AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031355

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031355.t1 MTR_2g081090 99.248 266 0 1 1 266 1 264 0.0 533
MS.gene031355.t1 MTR_2g081090 100.000 223 0 0 44 266 15 237 1.15e-165 458
MS.gene031355.t1 MTR_5g097280 69.288 267 69 6 10 266 2 265 8.06e-126 358
MS.gene031355.t1 MTR_4g094605 69.434 265 76 4 1 265 6 265 8.29e-122 348
MS.gene031355.t1 MTR_6g012080 78.899 218 44 2 48 265 49 264 1.00e-120 345
MS.gene031355.t1 MTR_6g012110 77.982 218 46 2 48 265 49 264 2.98e-119 342
MS.gene031355.t1 MTR_6g011890 77.523 218 47 2 48 265 49 264 4.82e-119 341
MS.gene031355.t1 MTR_6g011880 77.523 218 47 2 48 265 49 264 9.30e-119 340
MS.gene031355.t1 MTR_6g011870 77.523 218 47 2 48 265 49 264 9.61e-119 340
MS.gene031355.t1 MTR_6g043600 67.982 228 51 2 38 265 23 228 3.04e-102 297
MS.gene031355.t1 MTR_6g060175 48.361 244 110 6 16 254 42 274 2.41e-58 187
MS.gene031355.t1 MTR_1g026550 40.230 261 141 4 9 254 62 322 1.05e-57 187
MS.gene031355.t1 MTR_6g060175 46.721 244 104 7 16 254 42 264 1.12e-51 170
MS.gene031355.t1 MTR_5g098785 41.014 217 95 8 61 254 63 269 8.22e-37 132
MS.gene031355.t1 MTR_5g098780 41.014 217 95 9 61 254 61 267 5.76e-36 129
MS.gene031355.t1 MTR_8g023790 34.274 248 124 10 19 250 32 256 4.96e-33 121
MS.gene031355.t1 MTR_3g101670 37.624 202 94 5 65 250 68 253 6.30e-32 119
MS.gene031355.t1 MTR_6g033320 37.557 221 109 9 37 254 39 233 1.34e-30 114
MS.gene031355.t1 MTR_2g042720 35.909 220 97 7 51 250 60 255 2.01e-30 114
MS.gene031355.t1 MTR_4g015570 35.622 233 92 7 62 251 61 278 4.66e-29 111
MS.gene031355.t1 MTR_3g435460 37.255 204 92 7 64 253 62 243 2.14e-27 106
MS.gene031355.t1 MTR_1g026550 33.721 172 105 3 9 171 62 233 8.53e-27 104
MS.gene031355.t1 MTR_3g435460 36.224 196 89 7 72 253 9 182 1.82e-23 94.7
MS.gene031355.t1 MTR_3g070340 41.611 149 72 5 92 240 137 270 5.84e-22 92.4
MS.gene031355.t1 MTR_2g008610 34.615 208 86 7 65 243 73 259 6.14e-21 89.4
MS.gene031355.t1 MTR_5g098770 48.750 80 40 1 183 262 15 93 7.13e-20 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031355.t1 AT5G54270 87.925 265 30 2 4 266 1 265 1.92e-174 481
MS.gene031355.t1 AT2G05070 68.421 266 71 6 10 265 2 264 5.71e-124 353
MS.gene031355.t1 AT2G05100 68.914 267 70 6 10 266 2 265 8.01e-124 353
MS.gene031355.t1 AT3G27690 68.302 265 79 5 2 265 5 265 2.07e-122 350
MS.gene031355.t1 AT3G27690 68.302 265 79 5 2 265 37 297 2.47e-122 351
MS.gene031355.t1 AT1G29930 70.539 241 58 4 36 265 28 266 1.03e-117 338
MS.gene031355.t1 AT1G29920 70.539 241 58 4 36 265 28 266 1.20e-117 338
MS.gene031355.t1 AT1G29910 70.539 241 58 4 36 265 28 266 1.20e-117 338
MS.gene031355.t1 AT2G34420 66.543 269 73 6 8 265 2 264 1.30e-117 338
MS.gene031355.t1 AT2G34430 71.967 239 55 4 37 265 29 265 1.89e-117 337
MS.gene031355.t1 AT2G05100 62.030 266 84 7 10 261 2 264 4.20e-103 303
MS.gene031355.t1 AT1G76570 46.977 215 106 2 48 254 106 320 3.44e-59 191
MS.gene031355.t1 AT4G10340 49.524 210 97 4 48 254 62 265 4.24e-53 174
MS.gene031355.t1 AT1G61520 42.857 217 91 9 61 254 59 265 2.59e-39 138
MS.gene031355.t1 AT1G61520 42.857 217 91 9 61 254 59 265 2.59e-39 138
MS.gene031355.t1 AT1G61520 42.029 207 87 9 71 254 14 210 5.60e-35 125
MS.gene031355.t1 AT3G61470 38.140 215 99 10 51 250 50 245 4.17e-34 124
MS.gene031355.t1 AT1G45474 40.206 194 101 4 65 250 56 242 3.73e-33 121
MS.gene031355.t1 AT1G45474 40.206 194 101 4 65 250 56 242 3.73e-33 121
MS.gene031355.t1 AT3G08940 35.622 233 92 7 62 251 59 276 6.91e-31 116
MS.gene031355.t1 AT3G54890 40.206 194 96 8 65 254 55 232 1.55e-30 114
MS.gene031355.t1 AT1G19150 35.111 225 100 8 51 254 63 262 2.57e-28 109
MS.gene031355.t1 AT3G47470 37.255 204 92 6 64 253 63 244 1.99e-27 106
MS.gene031355.t1 AT5G01530 34.322 236 97 7 62 254 62 282 2.05e-27 107
MS.gene031355.t1 AT1G15820 35.096 208 85 7 65 243 70 256 3.59e-21 90.1
MS.gene031355.t1 AT2G40100 41.447 152 73 5 92 243 136 271 7.45e-21 89.7
MS.gene031355.t1 AT3G54890 37.975 158 79 7 65 219 55 196 1.54e-19 84.7
MS.gene031355.t1 AT5G28450 48.718 78 36 2 174 250 87 161 1.50e-14 70.1

Find 93 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGGTGGTCTTGACTATTT+AGG 0.130970 2.2:+15000577 MS.gene031355:CDS
CTGTTCTTGGCAAATGCTTC+TGG 0.168930 2.2:-15000250 None:intergenic
GGCATAAACCCAGGCATTAT+TGG 0.260488 2.2:-15000914 None:intergenic
AGGACCTTTGGAGAATCTTT+TGG 0.271440 2.2:+15000870 MS.gene031355:CDS
GGAGAATTCCCTGGTGATTA+TGG 0.284769 2.2:+15000199 MS.gene031355:CDS
ACCAGACAGAGTGAAATATT+TGG 0.293839 2.2:+15000141 MS.gene031355:CDS
AGGTTTCCAAGTTGTTCTAA+TGG 0.320920 2.2:+15000642 MS.gene031355:CDS
CCAGACAGAGTGAAATATTT+GGG 0.332386 2.2:+15000142 MS.gene031355:CDS
AACCTCAAGAGCCCTGTTCT+TGG 0.335763 2.2:-15000263 None:intergenic
AAGGTGGTCTTGACTATTTA+GGG 0.341687 2.2:+15000578 MS.gene031355:CDS
TTAAGCTCAGCGAAAGTAAC+TGG 0.345168 2.2:-15000769 None:intergenic
CTTTGTGCATGAACAAGGTT+TGG 0.349100 2.2:-15000604 None:intergenic
TTCCGCATCAACGGTCTTCC+TGG 0.355033 2.2:+15000679 MS.gene031355:CDS
GGTTTCCAAGTTGTTCTAAT+GGG 0.357946 2.2:+15000643 MS.gene031355:CDS
ACCCAGGCATTATTGGCAAC+AGG 0.364495 2.2:-15000907 None:intergenic
CCCAAATATTTCACTCTGTC+TGG 0.371437 2.2:-15000142 None:intergenic
CAGAAGCATTTGCCAAGAAC+AGG 0.372548 2.2:+15000251 MS.gene031355:CDS
TGTCACTGGTAAAGGACCTT+TGG 0.380892 2.2:+15000858 MS.gene031355:CDS
TTGATTTCAAAGAACCAGTT+TGG 0.381372 2.2:+15000527 MS.gene031355:CDS
CAGACTCCTTCATACTTAAC+TGG 0.384985 2.2:+15000178 MS.gene031355:CDS
GGCCAATACTTTGACCCTCT+TGG 0.405341 2.2:+15000733 MS.gene031355:CDS
GAAGGAGTCTGAGCTGAAAA+TGG 0.411828 2.2:-15000166 None:intergenic
CAAAGCATCTTAGCAGTACT+AGG 0.430115 2.2:+15000622 MS.gene031355:CDS
CAAGCTATTGTCACTGGTAA+AGG 0.430252 2.2:+15000850 MS.gene031355:CDS
GATCCTGCTTTGAACCAAAC+TGG 0.431531 2.2:-15000541 None:intergenic
GGAAATCAAGAATGGAAGAT+TGG 0.436306 2.2:+15000798 MS.gene031355:CDS
TATGCCACCAAGTTTGTGCC+TGG 0.443893 2.2:+15000931 MS.gene031355:CDS
GTTAAACAAACTCCTTTCCT+TGG 0.446741 2.2:+14999457 MS.gene031355:CDS
TGAAGGTGATCCATGGAAGA+TGG 0.451873 2.2:+15000446 MS.gene031355:intron
ACCCTGTTGCCAATAATGCC+TGG 0.466859 2.2:+15000905 MS.gene031355:CDS
TTTGTTCAAGCTATTGTCAC+TGG 0.469594 2.2:+15000844 MS.gene031355:CDS
GTTCTAATGGGTCTTGTCGA+AGG 0.474424 2.2:+15000655 MS.gene031355:CDS
CCCTGTTGCCAATAATGCCT+GGG 0.476932 2.2:+15000906 MS.gene031355:CDS
GGGTCAAAGTATTGGCCACC+AGG 0.488607 2.2:-15000727 None:intergenic
GACCAAGAGGGTCAAAGTAT+TGG 0.490473 2.2:-15000735 None:intergenic
TCCTTTCCTTGGTCAAAGGA+AGG 0.490595 2.2:+14999468 MS.gene031355:CDS
AGTATTGGCCACCAGGGTAA+AGG 0.501836 2.2:-15000720 None:intergenic
TTCTAATGGGTCTTGTCGAA+GGG 0.505605 2.2:+15000656 MS.gene031355:CDS
TTTGTGCATGAACAAGGTTT+GGG 0.509110 2.2:-15000603 None:intergenic
CAAGACCCATTAGAACAACT+TGG 0.511234 2.2:-15000648 None:intergenic
AAACTCCTTTCCTTGGTCAA+AGG 0.511273 2.2:+14999464 MS.gene031355:CDS
CAGCACCCTTCCTTTGACCA+AGG 0.515079 2.2:-14999474 None:intergenic
TGCCAAGAACAGGGCTCTTG+AGG 0.515098 2.2:+15000261 MS.gene031355:CDS
TTTCGCTGAGCTTAAAGTGA+AGG 0.516818 2.2:+15000777 MS.gene031355:CDS
TTAAGAACCAGGCACAAACT+TGG 0.519988 2.2:-15000938 None:intergenic
CTAGCATAGCCCATCTTCCA+TGG 0.521302 2.2:-15000456 None:intergenic
GTCGAAGGGTTCCGCATCAA+CGG 0.526007 2.2:+15000670 MS.gene031355:CDS
AATTCCCTGGTGATTATGGA+TGG 0.526464 2.2:+15000203 MS.gene031355:CDS
GAACCAGTTTGGTTCAAAGC+AGG 0.531298 2.2:+15000538 MS.gene031355:CDS
CTTAGAGATGTTGTCGCCAT+GGG 0.532010 2.2:+14999505 MS.gene031355:CDS
TACTTAACTGGAGAATTCCC+TGG 0.533200 2.2:+15000190 MS.gene031355:CDS
GAAGGTGATCCATGGAAGAT+GGG 0.534211 2.2:+15000447 MS.gene031355:intron
ATGGCGACAACATCTCTAAG+AGG 0.535432 2.2:-14999502 None:intergenic
TTGTTGCCCTCTCCGACACC+AGG 0.535866 2.2:-15000697 None:intergenic
GATTATGGATGGGACACTGC+TGG 0.539551 2.2:+15000214 MS.gene031355:CDS
AAAGTGAAGGAAATCAAGAA+TGG 0.545234 2.2:+15000790 MS.gene031355:CDS
GGTCTTCCTGGTGTCGGAGA+GGG 0.548663 2.2:+15000691 MS.gene031355:CDS
AGATGCTTTGTGCATGAACA+AGG 0.550454 2.2:-15000609 None:intergenic
TCTTAGAGATGTTGTCGCCA+TGG 0.551003 2.2:+14999504 MS.gene031355:CDS
CATGGAAGATGGGCTATGCT+AGG 0.554415 2.2:+15000457 MS.gene031355:CDS
AACATTGATGTTTAAGAACC+AGG 0.556979 2.2:-15000949 None:intergenic
AATTCTCCAGTTAAGTATGA+AGG 0.558241 2.2:-15000184 None:intergenic
GTGTCCCATCCATAATCACC+AGG 0.560079 2.2:-15000208 None:intergenic
CCTTTCCTTGGTCAAAGGAA+GGG 0.568157 2.2:+14999469 MS.gene031355:CDS
TGGGCTATGCTAGGAGCACT+CGG 0.571623 2.2:+15000466 MS.gene031355:CDS
GACAACATCTCTAAGAGGGT+TGG 0.572370 2.2:-14999497 None:intergenic
CGGTCTTCCTGGTGTCGGAG+AGG 0.575168 2.2:+15000690 MS.gene031355:CDS
ATCAACGGTCTTCCTGGTGT+CGG 0.580505 2.2:+15000685 MS.gene031355:CDS
AGCGAAAGTAACTGGATCGT+CGG 0.584697 2.2:-15000761 None:intergenic
CCCAGGCATTATTGGCAACA+GGG 0.587322 2.2:-15000906 None:intergenic
GGTGAATCTTGAGGTTCCCA+TGG 0.597497 2.2:-14999521 None:intergenic
AAATACCATGGTGAATCTTG+AGG 0.601532 2.2:-14999530 None:intergenic
AAGGGAAATGAATTGTGGTA+TGG 0.602138 2.2:+15000118 MS.gene031355:intron
TGTAGCAGCCATCAATGCCA+TGG 0.603074 2.2:-14999416 None:intergenic
AGAACCAGGCACAAACTTGG+TGG 0.606113 2.2:-15000935 None:intergenic
CCCTTCCTTTGACCAAGGAA+AGG 0.609595 2.2:-14999469 None:intergenic
GGATCGCAAATCTTCTCAGA+AGG 0.611974 2.2:+15000559 MS.gene031355:CDS
CACCAGGAAGACCGTTGATG+CGG 0.612040 2.2:-15000681 None:intergenic
TCGCAAATCTTCTCAGAAGG+TGG 0.619983 2.2:+15000562 MS.gene031355:CDS
ATTCCCTGGTGATTATGGAT+GGG 0.621810 2.2:+15000204 MS.gene031355:CDS
AGAAGCATTTGCCAAGAACA+GGG 0.625251 2.2:+15000252 MS.gene031355:CDS
TGGCGACAACATCTCTAAGA+GGG 0.635101 2.2:-14999501 None:intergenic
TTGTGAAGGGAAATGAATTG+TGG 0.635151 2.2:+15000113 MS.gene031355:intron
GGATCGTCGGCTAGACCAAG+AGG 0.652317 2.2:-15000748 None:intergenic
GGTCAAAGTATTGGCCACCA+GGG 0.653321 2.2:-15000726 None:intergenic
TGATCCAAAAGATTCTCCAA+AGG 0.654984 2.2:-15000874 None:intergenic
AAACTTGGTGGCATAAACCC+AGG 0.664430 2.2:-15000923 None:intergenic
GAGGGCAACAACCTTTACCC+TGG 0.674842 2.2:+15000709 MS.gene031355:CDS
GATCGTCGGCTAGACCAAGA+GGG 0.686043 2.2:-15000747 None:intergenic
GGCAACAACCTTTACCCTGG+TGG 0.691666 2.2:+15000712 MS.gene031355:CDS
GGGAACCTCAAGATTCACCA+TGG 0.716738 2.2:+14999525 MS.gene031355:CDS
CAGACAGAGTGAAATATTTG+GGG 0.720681 2.2:+15000143 MS.gene031355:CDS
TGTCCCATCCATAATCACCA+GGG 0.728458 2.2:-15000207 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTATACTAAAAGAAAA+AGG - chr2.2:14999565-14999584 None:intergenic 10.0%
!! AATTTATACTAAAAGAAAAA+GGG - chr2.2:14999564-14999583 None:intergenic 10.0%
!! ACATAACTTAATATCTTTAT+CGG - chr2.2:15000419-15000438 None:intergenic 15.0%
!! TTTAGAAAAAAACATAAGAA+TGG - chr2.2:15000335-15000354 None:intergenic 15.0%
!!! ACGTTTAGATATGTTTTTAT+TGG + chr2.2:14999990-15000009 MS.gene031355:intron 20.0%
!!! CAAGTATGTTTTTTTATCAT+TGG + chr2.2:14999745-14999764 MS.gene031355:intron 20.0%
!!! TAAGTTATGTTGAATTTTGA+AGG + chr2.2:15000429-15000448 MS.gene031355:intron 20.0%
!!! TAGAACGTTAACAAAATTTT+TGG - chr2.2:15000369-15000388 None:intergenic 20.0%
! CACTATGCTAACATATTTAT+AGG + chr2.2:14999585-14999604 MS.gene031355:intron 25.0%
! CTAACAAAGTATATGCTTTA+TGG - chr2.2:14999876-14999895 None:intergenic 25.0%
! TAAAAAAACATACTTGTGCA+TGG - chr2.2:14999741-14999760 None:intergenic 25.0%
!!! TTTATATAGTGTTGCTTATG+TGG + chr2.2:15000066-15000085 MS.gene031355:intron 25.0%
AAAGTGAAGGAAATCAAGAA+TGG + chr2.2:15000790-15000809 MS.gene031355:CDS 30.0%
AATGGAATGAGAAAAAACCA+TGG - chr2.2:15000317-15000336 None:intergenic 30.0%
AATTCTCCAGTTAAGTATGA+AGG - chr2.2:15000187-15000206 None:intergenic 30.0%
ATGCTTTATGGCCAATTATA+TGG - chr2.2:14999864-14999883 None:intergenic 30.0%
ATTGTGTTTGTGATTGTGAA+GGG + chr2.2:15000100-15000119 MS.gene031355:intron 30.0%
CTAAAAGAAAAAGGGAAAAG+AGG - chr2.2:14999556-14999575 None:intergenic 30.0%
TAACTAATGATAGACACGTT+CGG + chr2.2:14999946-14999965 MS.gene031355:intron 30.0%
TTAAAAATGCTCCAAAACCA+TGG + chr2.2:15000297-15000316 MS.gene031355:intron 30.0%
TTGATTTCAAAGAACCAGTT+TGG + chr2.2:15000527-15000546 MS.gene031355:CDS 30.0%
TTTGTTTCGCACCATATAAT+TGG + chr2.2:14999850-14999869 MS.gene031355:intron 30.0%
! ACCTGAAGTACTTGAAAAAT+GGG + chr2.2:15000498-15000517 MS.gene031355:CDS 30.0%
! ATGAGAAAAAACCATGGTTT+TGG - chr2.2:15000311-15000330 None:intergenic 30.0%
AAACGTGTGATGCTACTAAT+TGG + chr2.2:14999631-14999650 MS.gene031355:intron 35.0%
AAATACCATGGTGAATCTTG+AGG - chr2.2:14999533-14999552 None:intergenic 35.0%
AACTAATGATAGACACGTTC+GGG + chr2.2:14999947-14999966 MS.gene031355:intron 35.0%
AAGGGAAATGAATTGTGGTA+TGG + chr2.2:15000118-15000137 MS.gene031355:intron 35.0%
ACCAGACAGAGTGAAATATT+TGG + chr2.2:15000141-15000160 MS.gene031355:CDS 35.0%
AGGTTTCCAAGTTGTTCTAA+TGG + chr2.2:15000642-15000661 MS.gene031355:CDS 35.0%
CAGACAGAGTGAAATATTTG+GGG + chr2.2:15000143-15000162 MS.gene031355:CDS 35.0%
CATTGTGTTTGTGATTGTGA+AGG + chr2.2:15000099-15000118 MS.gene031355:intron 35.0%
CCAGACAGAGTGAAATATTT+GGG + chr2.2:15000142-15000161 MS.gene031355:CDS 35.0%
GGAAATCAAGAATGGAAGAT+TGG + chr2.2:15000798-15000817 MS.gene031355:CDS 35.0%
GGTTTCCAAGTTGTTCTAAT+GGG + chr2.2:15000643-15000662 MS.gene031355:CDS 35.0%
GTTAAACAAACTCCTTTCCT+TGG + chr2.2:14999457-14999476 MS.gene031355:CDS 35.0%
TGATCCAAAAGATTCTCCAA+AGG - chr2.2:15000877-15000896 None:intergenic 35.0%
TTAGTAGCATCACACGTTTA+AGG - chr2.2:14999630-14999649 None:intergenic 35.0%
TTGTGAAGGGAAATGAATTG+TGG + chr2.2:15000113-15000132 MS.gene031355:intron 35.0%
TTTGTGCATGAACAAGGTTT+GGG - chr2.2:15000606-15000625 None:intergenic 35.0%
TTTGTTCAAGCTATTGTCAC+TGG + chr2.2:15000844-15000863 MS.gene031355:CDS 35.0%
! AAGGTGGTCTTGACTATTTA+GGG + chr2.2:15000578-15000597 MS.gene031355:CDS 35.0%
! CACCTGAAGTACTTGAAAAA+TGG + chr2.2:15000497-15000516 MS.gene031355:CDS 35.0%
! TTGGCTATGTTTTCGATGTT+TGG + chr2.2:15000817-15000836 MS.gene031355:CDS 35.0%
!! TGAATTTTGAAGGTGATCCA+TGG + chr2.2:15000439-15000458 MS.gene031355:intron 35.0%
!!! ACCCATTTTTCAAGTACTTC+AGG - chr2.2:15000502-15000521 None:intergenic 35.0%
AAACTCCTTTCCTTGGTCAA+AGG + chr2.2:14999464-14999483 MS.gene031355:CDS 40.0%
AATTCCCTGGTGATTATGGA+TGG + chr2.2:15000203-15000222 MS.gene031355:CDS 40.0%
AGATGCTTTGTGCATGAACA+AGG - chr2.2:15000612-15000631 None:intergenic 40.0%
AGGGAAAAGAGGAAATACCA+TGG - chr2.2:14999545-14999564 None:intergenic 40.0%
ATTCCCTGGTGATTATGGAT+GGG + chr2.2:15000204-15000223 MS.gene031355:CDS 40.0%
CAAGACCCATTAGAACAACT+TGG - chr2.2:15000651-15000670 None:intergenic 40.0%
CAAGCTATTGTCACTGGTAA+AGG + chr2.2:15000850-15000869 MS.gene031355:CDS 40.0%
CAGACTCCTTCATACTTAAC+TGG + chr2.2:15000178-15000197 MS.gene031355:CDS 40.0%
CCCAAATATTTCACTCTGTC+TGG - chr2.2:15000145-15000164 None:intergenic 40.0%
CTTTGTGCATGAACAAGGTT+TGG - chr2.2:15000607-15000626 None:intergenic 40.0%
TACTTAACTGGAGAATTCCC+TGG + chr2.2:15000190-15000209 MS.gene031355:CDS 40.0%
TTAAGAACCAGGCACAAACT+TGG - chr2.2:15000941-15000960 None:intergenic 40.0%
TTAAGCTCAGCGAAAGTAAC+TGG - chr2.2:15000772-15000791 None:intergenic 40.0%
TTTCGCTGAGCTTAAAGTGA+AGG + chr2.2:15000777-15000796 MS.gene031355:CDS 40.0%
! CAAAGCATCTTAGCAGTACT+AGG + chr2.2:15000622-15000641 MS.gene031355:CDS 40.0%
! GAAGGTGGTCTTGACTATTT+AGG + chr2.2:15000577-15000596 MS.gene031355:CDS 40.0%
! TTCTAATGGGTCTTGTCGAA+GGG + chr2.2:15000656-15000675 MS.gene031355:CDS 40.0%
!! AGAAGCATTTGCCAAGAACA+GGG + chr2.2:15000252-15000271 MS.gene031355:CDS 40.0%
!! AGGACCTTTGGAGAATCTTT+TGG + chr2.2:15000870-15000889 MS.gene031355:CDS 40.0%
AGCGAAAGTAACTGGATCGT+CGG - chr2.2:15000764-15000783 None:intergenic 45.0%
ATGGCGACAACATCTCTAAG+AGG - chr2.2:14999505-14999524 None:intergenic 45.0%
CCTTTCCTTGGTCAAAGGAA+GGG + chr2.2:14999469-14999488 MS.gene031355:CDS 45.0%
CTGTTCTTGGCAAATGCTTC+TGG - chr2.2:15000253-15000272 None:intergenic 45.0%
CTTAGAGATGTTGTCGCCAT+GGG + chr2.2:14999505-14999524 MS.gene031355:CDS 45.0%
GAAGGAGTCTGAGCTGAAAA+TGG - chr2.2:15000169-15000188 None:intergenic 45.0%
GAAGGTGATCCATGGAAGAT+GGG + chr2.2:15000447-15000466 MS.gene031355:intron 45.0%
GACCAAGAGGGTCAAAGTAT+TGG - chr2.2:15000738-15000757 None:intergenic 45.0%
GGAGAATTCCCTGGTGATTA+TGG + chr2.2:15000199-15000218 MS.gene031355:CDS 45.0%
GGATCGCAAATCTTCTCAGA+AGG + chr2.2:15000559-15000578 MS.gene031355:CDS 45.0%
GGCATAAACCCAGGCATTAT+TGG - chr2.2:15000917-15000936 None:intergenic 45.0%
TCCTTTCCTTGGTCAAAGGA+AGG + chr2.2:14999468-14999487 MS.gene031355:CDS 45.0%
TCGCAAATCTTCTCAGAAGG+TGG + chr2.2:15000562-15000581 MS.gene031355:CDS 45.0%
TCTTAGAGATGTTGTCGCCA+TGG + chr2.2:14999504-14999523 MS.gene031355:CDS 45.0%
TGAAGGTGATCCATGGAAGA+TGG + chr2.2:15000446-15000465 MS.gene031355:intron 45.0%
TGTCACTGGTAAAGGACCTT+TGG + chr2.2:15000858-15000877 MS.gene031355:CDS 45.0%
TGTCCCATCCATAATCACCA+GGG - chr2.2:15000210-15000229 None:intergenic 45.0%
! AAACTTGGTGGCATAAACCC+AGG - chr2.2:15000926-15000945 None:intergenic 45.0%
! GAACCAGTTTGGTTCAAAGC+AGG + chr2.2:15000538-15000557 MS.gene031355:CDS 45.0%
! GACAACATCTCTAAGAGGGT+TGG - chr2.2:14999500-14999519 None:intergenic 45.0%
! GATCCTGCTTTGAACCAAAC+TGG - chr2.2:15000544-15000563 None:intergenic 45.0%
! GTTCTAATGGGTCTTGTCGA+AGG + chr2.2:15000655-15000674 MS.gene031355:CDS 45.0%
! TGGCGACAACATCTCTAAGA+GGG - chr2.2:14999504-14999523 None:intergenic 45.0%
!! CAGAAGCATTTGCCAAGAAC+AGG + chr2.2:15000251-15000270 MS.gene031355:CDS 45.0%
AACCTCAAGAGCCCTGTTCT+TGG - chr2.2:15000266-15000285 None:intergenic 50.0%
ACCCAGGCATTATTGGCAAC+AGG - chr2.2:15000910-15000929 None:intergenic 50.0%
ACCCTGTTGCCAATAATGCC+TGG + chr2.2:15000905-15000924 MS.gene031355:CDS 50.0%
AGAACCAGGCACAAACTTGG+TGG - chr2.2:15000938-15000957 None:intergenic 50.0%
ATCAACGGTCTTCCTGGTGT+CGG + chr2.2:15000685-15000704 MS.gene031355:CDS 50.0%
CCCAGGCATTATTGGCAACA+GGG - chr2.2:15000909-15000928 None:intergenic 50.0%
CCCTGTTGCCAATAATGCCT+GGG + chr2.2:15000906-15000925 MS.gene031355:CDS 50.0%
CCCTTCCTTTGACCAAGGAA+AGG - chr2.2:14999472-14999491 None:intergenic 50.0%
CTAGCATAGCCCATCTTCCA+TGG - chr2.2:15000459-15000478 None:intergenic 50.0%
GATTATGGATGGGACACTGC+TGG + chr2.2:15000214-15000233 MS.gene031355:CDS 50.0%
GGCCAATACTTTGACCCTCT+TGG + chr2.2:15000733-15000752 MS.gene031355:CDS 50.0%
GGGAACCTCAAGATTCACCA+TGG + chr2.2:14999525-14999544 MS.gene031355:CDS 50.0%
GGTGAATCTTGAGGTTCCCA+TGG - chr2.2:14999524-14999543 None:intergenic 50.0%
GTGTCCCATCCATAATCACC+AGG - chr2.2:15000211-15000230 None:intergenic 50.0%
TATGCCACCAAGTTTGTGCC+TGG + chr2.2:15000931-15000950 MS.gene031355:CDS 50.0%
!! AGTATTGGCCACCAGGGTAA+AGG - chr2.2:15000723-15000742 None:intergenic 50.0%
!! CATGGAAGATGGGCTATGCT+AGG + chr2.2:15000457-15000476 MS.gene031355:CDS 50.0%
!! GGTCAAAGTATTGGCCACCA+GGG - chr2.2:15000729-15000748 None:intergenic 50.0%
CACCAGGAAGACCGTTGATG+CGG - chr2.2:15000684-15000703 None:intergenic 55.0%
CAGCACCCTTCCTTTGACCA+AGG - chr2.2:14999477-14999496 None:intergenic 55.0%
GAGGGCAACAACCTTTACCC+TGG + chr2.2:15000709-15000728 MS.gene031355:CDS 55.0%
GGCAACAACCTTTACCCTGG+TGG + chr2.2:15000712-15000731 MS.gene031355:CDS 55.0%
GTCGAAGGGTTCCGCATCAA+CGG + chr2.2:15000670-15000689 MS.gene031355:CDS 55.0%
TGCCAAGAACAGGGCTCTTG+AGG + chr2.2:15000261-15000280 MS.gene031355:CDS 55.0%
TTCCGCATCAACGGTCTTCC+TGG + chr2.2:15000679-15000698 MS.gene031355:CDS 55.0%
! GATCGTCGGCTAGACCAAGA+GGG - chr2.2:15000750-15000769 None:intergenic 55.0%
!! GGGTCAAAGTATTGGCCACC+AGG - chr2.2:15000730-15000749 None:intergenic 55.0%
!! TGGGCTATGCTAGGAGCACT+CGG + chr2.2:15000466-15000485 MS.gene031355:CDS 55.0%
TTGTTGCCCTCTCCGACACC+AGG - chr2.2:15000700-15000719 None:intergenic 60.0%
! GGATCGTCGGCTAGACCAAG+AGG - chr2.2:15000751-15000770 None:intergenic 60.0%
! GGTCTTCCTGGTGTCGGAGA+GGG + chr2.2:15000691-15000710 MS.gene031355:CDS 60.0%
! CGGTCTTCCTGGTGTCGGAG+AGG + chr2.2:15000690-15000709 MS.gene031355:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 14999418 15000960 14999418 ID=MS.gene031355
chr2.2 mRNA 14999418 15000960 14999418 ID=MS.gene031355.t1;Parent=MS.gene031355
chr2.2 exon 14999418 14999546 14999418 ID=MS.gene031355.t1.exon1;Parent=MS.gene031355.t1
chr2.2 CDS 14999418 14999546 14999418 ID=cds.MS.gene031355.t1;Parent=MS.gene031355.t1
chr2.2 exon 15000121 15000282 15000121 ID=MS.gene031355.t1.exon2;Parent=MS.gene031355.t1
chr2.2 CDS 15000121 15000282 15000121 ID=cds.MS.gene031355.t1;Parent=MS.gene031355.t1
chr2.2 exon 15000451 15000960 15000451 ID=MS.gene031355.t1.exon3;Parent=MS.gene031355.t1
chr2.2 CDS 15000451 15000960 15000451 ID=cds.MS.gene031355.t1;Parent=MS.gene031355.t1
Gene Sequence

>MS.gene031355

ATGGCATTGATGGCTGCTACAGCAAGTTCAGCAGCTGTTGTTAAACAAACTCCTTTCCTTGGTCAAAGGAAGGGTGCTGCCAACCCTCTTAGAGATGTTGTCGCCATGGGAACCTCAAGATTCACCATGGTATTTCCTCTTTTCCCTTTTTCTTTTAGTATAAATTTCACTATGCTAACATATTTATAGGAATAAGCATTCATTTTAGTCCTTAAACGTGTGATGCTACTAATTGGTCTAACTAACTAGTGTGACAATATTGTATATAATAAAGTATGATTTTGTCTAATGTGCACACAAGTAAAATTCACTTGCTTGCACCATGCACAAGTATGTTTTTTTATCATTGGATCAATAAGTAGCATCATATTTCATTCAATTTAATAGTGAGATTTATTGATCAAATTTGTAAAACTTATGCATGATGCAAGATTTGTTTCGCACCATATAATTGGCCATAAAGCATATACTTTGTTAGTAATGTTATAGACTAAATTGACTCAAGTTAGACACATTCAAAAACTAAACTAACTAATGATAGACACGTTCGGGAGTAAAATGACTATAAAAACACGTTTAGATATGTTTTTATTGGTTATACATGCAATGACTAGCACATTTAAGTAGTGAAATAATTGTCTACTTGTATTTATATAGTGTTGCTTATGTGGCTGATACATGCATTGTGTTTGTGATTGTGAAGGGAAATGAATTGTGGTATGGACCAGACAGAGTGAAATATTTGGGGCCATTTTCAGCTCAGACTCCTTCATACTTAACTGGAGAATTCCCTGGTGATTATGGATGGGACACTGCTGGTTTATCTGCTGATCCAGAAGCATTTGCCAAGAACAGGGCTCTTGAGGTTAGTTTTCATGTTTAAAAATGCTCCAAAACCATGGTTTTTTCTCATTCCATTCTTATGTTTTTTTCTAAATATTTCAATTACCAAAAATTTTGTTAACGTTCTAAAGAGTGAATAATTTATATTAGTGACACCGATAAAGATATTAAGTTATGTTGAATTTTGAAGGTGATCCATGGAAGATGGGCTATGCTAGGAGCACTCGGATGCATCACACCTGAAGTACTTGAAAAATGGGTGAGAGTTGATTTCAAAGAACCAGTTTGGTTCAAAGCAGGATCGCAAATCTTCTCAGAAGGTGGTCTTGACTATTTAGGGAACCCAAACCTTGTTCATGCACAAAGCATCTTAGCAGTACTAGGTTTCCAAGTTGTTCTAATGGGTCTTGTCGAAGGGTTCCGCATCAACGGTCTTCCTGGTGTCGGAGAGGGCAACAACCTTTACCCTGGTGGCCAATACTTTGACCCTCTTGGTCTAGCCGACGATCCAGTTACTTTCGCTGAGCTTAAAGTGAAGGAAATCAAGAATGGAAGATTGGCTATGTTTTCGATGTTTGGTTTCTTTGTTCAAGCTATTGTCACTGGTAAAGGACCTTTGGAGAATCTTTTGGATCACATTGACAACCCTGTTGCCAATAATGCCTGGGTTTATGCCACCAAGTTTGTGCCTGGTTCTTAA
Protein sequence

>MS.gene031355.t1

MALMAATASSAAVVKQTPFLGQRKGAANPLRDVVAMGTSRFTMGNELWYGPDRVKYLGPFSAQTPSYLTGEFPGDYGWDTAGLSADPEAFAKNRALEVIHGRWAMLGALGCITPEVLEKWVRVDFKEPVWFKAGSQIFSEGGLDYLGNPNLVHAQSILAVLGFQVVLMGLVEGFRINGLPGVGEGNNLYPGGQYFDPLGLADDPVTFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLENLLDHIDNPVANNAWVYATKFVPGS