AlfalfaGEDB | MS.gene20444

M. sativa cultivar XinJiangDaYe / MS.gene20444

KEGG: K08912
GO: -
PFAM: PF00504.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20444.t1	RHN50045.1	98.5	265	4	0	1	265	268	532	2.50E-148	534.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20444.t1	P07369	92.1	267	19	2	1	265	1	267	1.8e-142	506.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20444.t1	G7KKG9	98.5	265	4	0	1	265	1	265	1.8e-148	534.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene20444	0.821847	3.25E-53	-1.69E-46
MS.gene049173	MS.gene20444	0.838387	2.96E-57	-1.69E-46
MS.gene049217	MS.gene20444	0.843193	1.63E-58	-1.69E-46
MS.gene049222	MS.gene20444	0.815085	1.11E-51	-1.69E-46
MS.gene049232	MS.gene20444	0.828496	8.71E-55	-1.69E-46
MS.gene049285	MS.gene20444	0.890497	9.34E-74	-1.69E-46
MS.gene049320	MS.gene20444	0.908355	1.87E-81	-1.69E-46
MS.gene049600	MS.gene20444	0.822371	2.46E-53	-1.69E-46
MS.gene049609	MS.gene20444	0.80716	5.79E-50	-1.69E-46
MS.gene049805	MS.gene20444	0.809414	1.91E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene023132	MS.gene20444	PPI
MS.gene031016	MS.gene20444	PPI
MS.gene031028	MS.gene20444	PPI
MS.gene20444	MS.gene023132	PPI
MS.gene20444	MS.gene031355	PPI
MS.gene20444	MS.gene020883	PPI
MS.gene20444	MS.gene031028	PPI
MS.gene20444	MS.gene20445	PPI
MS.gene20444	MS.gene20443	PPI
MS.gene20444	MS.gene028321	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20444.t1	MTR_6g012080	98.491	265	4	0	1	265	1	265	0.0	531
MS.gene20444.t1	MTR_6g012110	96.226	265	10	0	1	265	1	265	0.0	521
MS.gene20444.t1	MTR_6g011890	95.472	265	12	0	1	265	1	265	0.0	511
MS.gene20444.t1	MTR_6g011880	93.962	265	16	0	1	265	1	265	0.0	499
MS.gene20444.t1	MTR_6g011870	93.962	265	16	0	1	265	1	265	0.0	499
MS.gene20444.t1	MTR_4g094605	91.353	266	22	1	1	265	1	266	1.31e-179	494
MS.gene20444.t1	MTR_5g097280	76.119	268	58	3	1	265	1	265	2.42e-149	418
MS.gene20444.t1	MTR_6g043600	78.868	265	20	3	1	265	1	229	2.06e-140	394
MS.gene20444.t1	MTR_2g081090	78.899	218	44	2	49	264	19	236	4.36e-120	343
MS.gene20444.t1	MTR_2g081090	78.899	218	44	2	49	264	46	263	6.68e-120	343

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20444.t1	AT1G29930	88.015	267	30	2	1	265	1	267	3.79e-172	476
MS.gene20444.t1	AT1G29920	87.640	267	31	2	1	265	1	267	2.52e-171	473
MS.gene20444.t1	AT1G29910	87.640	267	31	2	1	265	1	267	2.52e-171	473
MS.gene20444.t1	AT2G34430	88.015	267	29	3	1	265	1	266	1.19e-169	469
MS.gene20444.t1	AT2G34420	88.015	267	28	3	1	265	1	265	1.77e-169	469
MS.gene20444.t1	AT3G27690	78.229	271	48	4	2	265	32	298	3.22e-152	426
MS.gene20444.t1	AT3G27690	78.676	272	45	5	1	265	1	266	9.18e-152	424
MS.gene20444.t1	AT2G05070	77.985	268	53	3	1	265	1	265	1.01e-150	421
MS.gene20444.t1	AT2G05100	77.491	271	49	5	1	265	1	265	1.60e-149	418
MS.gene20444.t1	AT2G05100	76.955	243	44	5	1	237	1	237	4.48e-128	366

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Find 110 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCCTCTGGATCATCAGCG+AGG	0.746493	6.3:-6556058	None:intergenic
AAGCTTCCACCCGGGTAGAG+TGG	0.534876	6.3:-6556023	None:intergenic
AAGGTGAAAGAACTCAAGAA+TGG	0.584740	6.3:+6556092	MS.gene20444:CDS
AAGTACTTGGGCCCATTCTC+TGG	0.361014	6.3:+6555630	MS.gene20444:CDS
AATGGGTCAAAGCTTCCACC+CGG	0.596844	6.3:-6556032	None:intergenic
AATTCACCAGTGAGGTAAGA+TGG	0.486152	6.3:-6555660	None:intergenic
AATTTCCTGGTGACTATGGT+TGG	0.506431	6.3:+6555679	MS.gene20444:CDS
ACAAAGCATCCTTGCAATTT+GGG	0.331163	6.3:+6555926	MS.gene20444:CDS
ACACGGTCTGGACCGTACCA+CGG	0.551715	6.3:-6555606	None:intergenic
ACCAAGAAGAACGTTCCTTC+TGG	0.405063	6.3:+6555579	MS.gene20444:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGCTATGTTCTCTATGTT+TGG	+	chr6.3:6556119-6556138	MS.gene20444:CDS	30.0%
AAGGTGAAAGAACTCAAGAA+TGG	+	chr6.3:6556092-6556111	MS.gene20444:CDS	35.0%
GCTTGCCAAGTTATCTTAAT+GGG	+	chr6.3:6555948-6555967	MS.gene20444:CDS	35.0%
GGATCTCAAATCTTTAGTGA+AGG	+	chr6.3:6555864-6555883	MS.gene20444:CDS	35.0%
TCTCAAATCTTTAGTGAAGG+AGG	+	chr6.3:6555867-6555886	MS.gene20444:CDS	35.0%
TCTCTTCTTCAACAATGGTT+GGG	+	chr6.3:6555487-6555506	MS.gene20444:CDS	35.0%
TGAAAGAACTCAAGAATGGT+AGG	+	chr6.3:6556096-6556115	MS.gene20444:CDS	35.0%
AATTCACCAGTGAGGTAAGA+TGG	-	chr6.3:6555663-6555682	None:intergenic	40.0%
AATTTCCTGGTGACTATGGT+TGG	+	chr6.3:6555679-6555698	MS.gene20444:CDS	40.0%
ATCTTAATGGGAGCTGTTGA+GGG	+	chr6.3:6555960-6555979	MS.gene20444:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	6555465	6556262	6555465	ID=MS.gene20444
chr6.3	mRNA	6555465	6556262	6555465	ID=MS.gene20444.t1;Parent=MS.gene20444
chr6.3	exon	6555465	6556262	6555465	ID=MS.gene20444.t1.exon1;Parent=MS.gene20444.t1
chr6.3	CDS	6555465	6556262	6555465	ID=cds.MS.gene20444.t1;Parent=MS.gene20444.t1

Gene Sequence

>MS.gene20444

ATGGCTACATCAACAATGGCTCTCTCTTCTTCAACAATGGTTGGGAAGGCAATCAAGCTTTCTCCCTCTACTCCAGACCTTGGCATGGGAAGAATCACCATGAGGAAAACAACCACCAAGAAGAACGTTCCTTCTGGAAGCCCGTGGTACGGTCCAGACCGTGTCAAGTACTTGGGCCCATTCTCTGGTGAAGCTCCATCTTACCTCACTGGTGAATTTCCTGGTGACTATGGTTGGGACACTGCTGGGCTTTCTGCTGACCCCGAGACATTTGCCAAAAACCGTGAACTCGAGGTCATCCACAGTAGATGGGCTATGTTGGGTGCCTTGGGATGTGTCTTCCCTGAGCTCTTATCTCGCAATGGTGTCAAATTCGGCGAGGCTGTGTGGTTCAAGGCAGGATCTCAAATCTTTAGTGAAGGAGGACTTGACTACTTGGGTAACCCAAGCCTAGTCCATGCACAAAGCATCCTTGCAATTTGGGCTTGCCAAGTTATCTTAATGGGAGCTGTTGAGGGTTACCGTATTGCTGGTGGACCTCTTGGTGAGGTAGTCGACCCACTCTACCCGGGTGGAAGCTTTGACCCATTGGGCCTCGCTGATGATCCAGAGGCTTTTGCAGAGTTGAAGGTGAAAGAACTCAAGAATGGTAGGTTAGCTATGTTCTCTATGTTTGGATTTTTCGTTCAGGCCATTGTAACCGGAAAGGGACCTCTAGAGAACCTTGCTGACCATATTGCTGACCCCGTCAACAACAACGCATGGGCCTTTGCCACAAACTTTGTCCCCGGAAAGTGA

Protein sequence

>MS.gene20444.t1

MATSTMALSSSTMVGKAIKLSPSTPDLGMGRITMRKTTTKKNVPSGSPWYGPDRVKYLGPFSGEAPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHSRWAMLGALGCVFPELLSRNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVILMGAVEGYRIAGGPLGEVVDPLYPGGSFDPLGLADDPEAFAELKVKELKNGRLAMFSMFGFFVQAIVTGKGPLENLADHIADPVNNNAWAFATNFVPGK